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Spectral Analysis

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https://www.readbyqxmd.com/read/28335521/gene-transformation-induced-changes-in-chemical-functional-group-features-and-molecular-structure-conformation-in-alfalfa-plants-co-expressing-lc-bhlh-and-c1-myb-transcriptive-flavanoid-regulatory-genes-effects-of-single-gene-and-two-gene-insertion
#1
Ravindra G Heendeniya, Peiqiang Yu
Alfalfa (Medicago sativa L.) genotypes transformed with Lc-bHLH and Lc transcription genes were developed with the intention of stimulating proanthocyanidin synthesis in the aerial parts of the plant. To our knowledge, there are no studies on the effect of single-gene and two-gene transformation on chemical functional groups and molecular structure changes in these plants. The objective of this study was to use advanced molecular spectroscopy with multivariate chemometrics to determine chemical functional group intensity and molecular structure changes in alfalfa plants when co-expressing Lc-bHLH and C1-MYB transcriptive flavanoid regulatory genes in comparison with non-transgenic (NT) and AC Grazeland (ACGL) genotypes...
March 20, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28335487/electromagnetic-vortex-based-radar-imaging-using-a-single-receiving-antenna-theory-and-experimental-results
#2
Tiezhu Yuan, Hongqiang Wang, Yongqiang Cheng, Yuliang Qin
Radar imaging based on electromagnetic vortex can achieve azimuth resolution without relative motion. The present paper investigates this imaging technique with the use of a single receiving antenna through theoretical analysis and experimental results. Compared with the use of multiple receiving antennas, the echoes from a single receiver cannot be used directly for image reconstruction using Fourier method. The reason is revealed by using the point spread function. An additional phase is compensated for each mode before imaging process based on the array parameters and the elevation of the targets...
March 19, 2017: Sensors
https://www.readbyqxmd.com/read/28335464/a-geographic-information-assisted-temporal-mixture-analysis-for-addressing-the-issue-of-endmember-class-and-endmember-spectra-variability
#3
Wenliang Li, Changshan Wu
Spectral mixture analysis (SMA) is a common approach for parameterizing biophysical fractions of urban environment and widely applied in many fields. For successful SMA, the selection of endmember class and corresponding spectra has been assumed as the most important step. Thanks to the spatial heterogeneity of natural and urban landscapes, the variability of endmember class and corresponding spectra has been widely considered as the profound error source in SMA. To address the challenging problems, we proposed a geographic information-assisted temporal mixture analysis (GATMA)...
March 18, 2017: Sensors
https://www.readbyqxmd.com/read/28335453/a-spatially-offset-raman-spectroscopy-method-for-non-destructive-detection-of-gelatin-encapsulated-powders
#4
Kuanglin Chao, Sagar Dhakal, Jianwei Qin, Yankun Peng, Walter F Schmidt, Moon S Kim, Diane E Chan
Non-destructive subsurface detection of encapsulated, coated, or seal-packaged foods and pharmaceuticals can help prevent distribution and consumption of counterfeit or hazardous products. This study used a Spatially Offset Raman Spectroscopy (SORS) method to detect and identify urea, ibuprofen, and acetaminophen powders contained within one or more (up to eight) layers of gelatin capsules to demonstrate subsurface chemical detection and identification. A 785-nm point-scan Raman spectroscopy system was used to acquire spatially offset Raman spectra for an offset range of 0 to 10 mm from the surfaces of 24 encapsulated samples, using a step size of 0...
March 18, 2017: Sensors
https://www.readbyqxmd.com/read/28335224/structural-changes-induced-in-grapevine-vitis-vinifera-l-dna-by-femtosecond-ir-laser-pulses-a-surface-enhanced-raman-spectroscopic-study
#5
Nicoleta E Dina, Cristina M Muntean, Nicolae Leopold, Alexandra Fălămaș, Adela Halmagyi, Ana Coste
In this work, surface-enhanced Raman spectra of ten genomic DNAs extracted from leaf tissues of different grapevine (Vitis vinifera L.) varieties, respectively, are analyzed in the wavenumber range 300-1800 cm(-1). Furthermore, structural changes induced in grapevine genomic nucleic acids upon femtosecond (170 fs) infrared (IR) laser pulse irradiation (λ = 1100 nm) are discussed in detail for seven genomic DNAs, respectively. Surface-enhanced Raman spectroscopy (SERS) signatures, vibrational band assignments and structural characterization of genomic DNAs are reported for each case...
May 25, 2016: Nanomaterials
https://www.readbyqxmd.com/read/28335194/automatic-echographic-detection-of-halloysite-clay-nanotubes-in-a-low-concentration-range
#6
Francesco Conversano, Paola Pisani, Ernesto Casciaro, Marco Di Paola, Stefano Leporatti, Roberto Franchini, Alessandra Quarta, Giuseppe Gigli, Sergio Casciaro
Aim of this work was to investigate the automatic echographic detection of an experimental drug delivery agent, halloysite clay nanotubes (HNTs), by employing an innovative method based on advanced spectral analysis of the corresponding "raw" radiofrequency backscatter signals. Different HNT concentrations in a low range (5.5-66 × 10(10) part/mL, equivalent to 0.25-3.00 mg/mL) were dispersed in custom-designed tissue-mimicking phantoms and imaged through a clinically-available echographic device at a conventional ultrasound diagnostic frequency (10 MHz)...
April 11, 2016: Nanomaterials
https://www.readbyqxmd.com/read/28334006/assessing-various-infrared-ir-microscopic-imaging-techniques-for-post-mortem-interval-evaluation-of-human-skeletal-remains
#7
Claudia Woess, Seraphin Hubert Unterberger, Clemens Roider, Monika Ritsch-Marte, Nadin Pemberger, Jan Cemper-Kiesslich, Petra Hatzer-Grubwieser, Walther Parson, Johannes Dominikus Pallua
Due to the influence of many environmental processes, a precise determination of the post-mortem interval (PMI) of skeletal remains is known to be very complicated. Although methods for the investigation of the PMI exist, there still remains much room for improvement. In this study the applicability of infrared (IR) microscopic imaging techniques such as reflection-, ATR- and Raman- microscopic imaging for the estimation of the PMI of human skeletal remains was tested. PMI specific features were identified and visualized by overlaying IR imaging data with morphological tissue structures obtained using light microscopy to differentiate between forensic and archaeological bone samples...
2017: PloS One
https://www.readbyqxmd.com/read/28333967/reconstructing-spectral-cues-for-sound-localization-from-responses-to-rippled-noise-stimuli
#8
A John Van Opstal, Joyce Vliegen, Thamar Van Esch
Human sound localization in the mid-saggital plane (elevation) relies on an analysis of the idiosyncratic spectral shape cues provided by the head and pinnae. However, because the actual free-field stimulus spectrum is a-priori unknown to the auditory system, the problem of extracting the elevation angle from the sensory spectrum is ill-posed. Here we test different spectral localization models by eliciting head movements toward broad-band noise stimuli with randomly shaped, rippled amplitude spectra emanating from a speaker at a fixed location, while varying the ripple bandwidth between 1...
2017: PloS One
https://www.readbyqxmd.com/read/28333081/hyperspectral-monitoring-of-green-roof-vegetation-health-state-in-sub-mediterranean-climate-preliminary-results
#9
Patrizia Piro, Michele Porti, Simone Veltri, Emanuela Lupo, Monica Moroni
In urban and industrial environments, the constant increase of impermeable surfaces has produced drastic changes in the natural hydrological cycle. Decreasing green areas not only produce negative effects from a hydrological-hydraulic perspective, but also from an energy point of view, modifying the urban microclimate and generating, as shown in the literature, heat islands in our cities. In this context, green infrastructures may represent an environmental compensation action that can be used to re-equilibrate the hydrological and energy balance and reduce the impact of pollutant load on receiving water bodies...
March 23, 2017: Sensors
https://www.readbyqxmd.com/read/28332199/investigating-the-feasibility-of-classifying-breast-microcalcifications-using-photon-counting-spectral-mammography-a-simulation-study
#10
Bahaa Ghammraoui, Stephen J Glick
PURPOSE: A dual-energy material decomposition method using photon-counting spectral mammography was investigated as a non-invasive diagnostic approach to differentiate between Type I calcifications, consisting of calcium oxalate dihydrate or weddellite compounds that are more often associated with benign lesions, and Type II calcifications containing hydroxyapatite that are predominantly associated with malignant tumors. METHODS: The study was carried out by numerical simulation to assess the feasibility of the proposed approach...
March 22, 2017: Medical Physics
https://www.readbyqxmd.com/read/28332082/detection-of-terrain-indices-related-to-soil-salinity-and-mapping-salt-affected-soils-using-remote-sensing-and-geostatistical-techniques
#11
Hela Triki Fourati, Moncef Bouaziz, Mourad Benzina, Samir Bouaziz
Traditional surveying methods of soil properties over landscapes are dramatically cost and time-consuming. Thus, remote sensing is a proper choice for monitoring environmental problem. This research aims to study the effect of environmental factors on soil salinity and to map the spatial distribution of this salinity over the southern east part of Tunisia by means of remote sensing and geostatistical techniques. For this purpose, we used Advanced Spaceborne Thermal Emission and Reflection Radiometer data to depict geomorphological parameters: elevation, slope, plan curvature (PLC), profile curvature (PRC), and aspect...
April 2017: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/28331884/accuracy-assessment-and-characterization-of-x-ray-coded-aperture-coherent-scatter-spectral-imaging-for-breast-cancer-classification
#12
Manu N Lakshmanan, Joel A Greenberg, Ehsan Samei, Anuj J Kapadia
Although transmission-based x-ray imaging is the most commonly used imaging approach for breast cancer detection, it exhibits false negative rates higher than 15%. To improve cancer detection accuracy, x-ray coherent scatter computed tomography (CSCT) has been explored to potentially detect cancer with greater consistency. However, the 10-min scan duration of CSCT limits its possible clinical applications. The coded aperture coherent scatter spectral imaging (CACSSI) technique has been shown to reduce scan time through enabling single-angle imaging while providing high detection accuracy...
January 2017: Journal of Medical Imaging
https://www.readbyqxmd.com/read/28330736/hyperspectral-imaging-using-near-infrared-spectroscopy-to-monitor-coat-thickness-uniformity-in-the-manufacture-of-a-transdermal-drug-delivery-system
#13
Naresh Pavurala, Xiaoming Xu, Yellela S R Krishnaiah
Hyperspectral imaging using near infrared spectroscopy (NIRS) integrates spectroscopy and conventional imaging to obtain both spectral and spatial information of materials. The non-invasive and rapid nature of hyperspectral imaging using NIRS makes it a valuable process analytical technology (PAT) tool for in-process monitoring and control of the manufacturing process for transdermal drug delivery systems (TDS). The focus of this investigation was to develop and validate the use of Near Infra-red (NIR) hyperspectral imaging to monitor coat thickness uniformity, a critical quality attribute (CQA) for TDS...
March 19, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28330342/experimental-verification-of-the-cluster-model-of-ch3f-ortho-h2-n-in-solid-para-h2-by-using-mid-infrared-pump-probe-laser-spectroscopy
#14
Yuki Miyamoto, Asao Mizoguchi, Hideto Kanamori
The bleaching process in the C-F stretching mode (ν3 band) of CH3F-(ortho-H2)n [n = 0 and 1] clusters in solid para-H2 was monitored using pump and probe laser spectroscopy on the C-H stretching mode (ν1 and 2ν5 bands). From an analysis of the depleted spectral profiles, the transition frequency and linewidth of each cluster were directly determined. The results agree with the values previously derived from a deconvolution analysis of the broadened ν1/2ν5 spectrum observed by FTIR spectroscopy. The complementary increase and decrease between the n = 0 and 1 components were also verified through monitoring the ν1 and 2ν5 bands, which suggests a closed system among the CH3F-(ortho-H2)n clusters...
March 21, 2017: Journal of Chemical Physics
https://www.readbyqxmd.com/read/28330288/biofabrication-and-spectral-characterization-of-silver-nanoparticles-and-their-cytotoxic-studies-on-human-cd34-ve-stem-cells
#15
Venkata S Kotakadi, Susmila Aparna Gaddam, Sucharitha K Venkata, P V G K Sarma, D V R Sai Gopal
Biosynthesis of plant-mediated silver nanoparticles is gaining significant importance due to environmentally safe 'green method' and it is an efficient alternative method. In the present study, silver nanoparticles were synthesized by using root extract of Glycyrrhiza glabra an important medicinal plant. The AgNPs are characterized by spectral analysis; the surface plasmon resonance (SPR) peak of AgNPs showed maximum absorption at 445 nm. Fourier-transform infrared spectroscopy (FT-IR) data show that the O-H hydroxyl groups, carboxylic acids, ester and ether groups and C-O stretching of alcohols have been utilized in the formation of AgNPs...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330087/decolorization-and-biodegradation-of-remazol-reactive-dyes-by-clostridium-species
#16
Sanmuga Priya Ekambaram, Senthamil Selvan Perumal, Umayal Annamalai
Decolorisation and biodegradation efficacy of potential strains isolated from dyeing effluent collected from Tirupur region, Tamil Nadu, India were studied in remazol reactive dyes. Two potential strains Clostridium butyricum (EI05) and Clostridium acetobutylicum (EI25) identified by biochemical tests in our previous study were studied for their decolorising efficiency on various remazol reactive dyes (Remazol Blue RGB, Remazol Blue RR, Remazol Navy RGB and Remazol Orange RR). The synthetic dyes showed complete decolorization after 24-72 h by two potential strains EI05 and EI25...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330071/unexpected-formation-of-n-phenyl-thiophosphorohydrazidic-acid-o-s-dimethyl-ester-from-acephate-chemical-biotechnical-and-computational-study
#17
Vijay Kumar, Sukhmanpreet Kaur, Simranjeet Singh, Niraj Upadhyay
By the nucleophilic attack of phenylhydrazine on acephate in aqueous medium, a stable product N'-phenyl-thiophosphorohydrazidic acid O,S-dimethyl ester (1) was obtained and characterized by elemental, spectral and thermal analysis. Thermodynamic parameters, Ea, ΔH°, ΔS° and ΔG°, have found to be 62.15, -67.95, -0.068 and -20.05 kJ mol(-1) according to the Coats-Redfern equation. Analysis of interaction of 1 with BSA protein was done by using the UV-Vis and FTIR spectroscopic methods. The observed binding constants was 1...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28329723/analysis-of-synthetic-phenethylamine-street-drugs-using-direct-sample-analysis-coupled-to-accurate-mass-time-of-flight-mass-spectrometry
#18
Maura K McGonigal, Joshua A Wilhide, Philip B Smith, Noelle M Elliott, Frank L Dorman
Serious health complications and fatal overdoses have brought phenethylamine, otherwise known as 2C, drug abuse to the public's attention. These compounds are 2C-X series analogs of mescaline. The name "2C" results from the two carbons in the ethyl chain. The substituents on these emerging drugs are constantly changed in order to avoid listing as controlled substances. These synthetic drugs are marketed as having affects similar to LSD and MDMA and are typically consumed sublingually via blotter paper. Twenty-six commercially available standards were analyzed using a direct sample analysis technique coupled to accurate mass time of flight mass spectrometry...
March 8, 2017: Forensic Science International
https://www.readbyqxmd.com/read/28328993/agreement-between-functional-connectivity-and-cortical-thickness-driven-correlation-maps-of-the-medial-frontal-cortex
#19
Hyunjin Park, Yeong-Hun Park, Jungho Cha, Sang Won Seo, Duk L Na, Jong-Min Lee
Parcellation of the human cortex has important implications in neuroscience. Parcellation is often a crucial requirement before meaningful regional analysis can occur. The human cortex can be parcellated into distinct regions based on structural features, such as gyri and sulci. Brain network patterns in a given region with respect to its neighbors, known as connectional fingerprints, can be used to parcellate the cortex. Distinct imaging modalities might provide complementary information for brain parcellation...
2017: PloS One
https://www.readbyqxmd.com/read/28328227/ultrafast-relaxation-dynamics-of-photoexcited-heme-model-compounds-observation-of-multiple-electronic-spin-states-and-vibrational-cooling
#20
Chinju Govind, Venugopal Karunakaran
Hemin is a unique model compound of heme proteins carrying out variable biological functions. Here the excited state relaxation dynamics of heme model compounds in the ferric form are systematically investigated by changing the axial ligand (Cl/Br), the peripheral substituent (Vinyl/Ethylmeso) and the solvent (Methanol/DMSO) using femtosecond pump-probe spectroscopy upon excitation at 380 nm. The relaxation time constants of these model compounds are obtained by global analysis. Excited state deactivation pathway of the model compounds comprising the decay of the porphyrin excited state (S*) to Ligand to Metal Charge Transfer state (LMCT, t1), back electron transfer from Metal to Ligand (MLCT, t2) and relaxation to the ground state through different electronic spin states of iron (t3 and t4), is proposed along with the vibrational cooling processes...
March 22, 2017: Journal of Physical Chemistry. B
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