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https://www.readbyqxmd.com/read/27911413/fabricating-a-uv-vis-and-raman-spectroscopy-immunoassay-platform
#1
Cynthia Hanson, Nathan D Israelsen, Michael Sieverts, Elizabeth Vargis
Immunoassays are used to detect proteins based on the presence of associated antibodies. Because of their extensive use in research and clinical settings, a large infrastructure of immunoassay instruments and materials can be found. For example, 96- and 384-well polystyrene plates are available commercially and have a standard design to accommodate ultraviolet-visible (UV-Vis) spectroscopy machines from various manufacturers. In addition, a wide variety of immunoglobulins, detection tags, and blocking agents for customized immunoassay designs such as enzyme-linked immunosorbent assays (ELISA) are available...
November 10, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27911114/changes-of-tangential-traction-after-macular-peeling-correlation-between-en-face-analysis-and-macular-sensitivity
#2
Mario R Romano, Gilda Cennamo, Ida Cesarano, Domenico Cardone, Giuseppe Nicoletti, Rodolfo Mastropasqua, Giovanni Cennamo
PURPOSE: To assess the epi- and intra-retinal morphology changes of the tangential tractions following vitreoretinal surgery for epiretinal membranes and to investigate the correlation between the depth of retinal changes and functional macular sensitivity (MS). METHODS: This is a prospective cross-sectional interventional study, in which 73 eyes of 73 patients with diagnosis of idiopathic epiretinal membrane (iERM) were evaluated. All patients received complete ophthalmic evaluation, including axial and en-face scanning spectral domain-optical coherence tomography (SD-OCT) analysis and microperimetry...
December 2, 2016: Current Eye Research
https://www.readbyqxmd.com/read/27910790/exploring-geographical-differentiation-of-the-hoelen-medicinal-mushroom-wolfiporia-extensa-agaricomycetes-using-fourier-transform-infrared-spectroscopy-combined-with-multivariate-analysis
#3
Yan Li, Ji Zhang, Yanli Zhao, Honggao Liu, Yuanzhong Wang, Hang Jin
In this study the geographical differentiation of dried sclerotia of the medicinal mushroom Wolfiporia extensa, obtained from different regions in Yunnan Province, China, was explored using Fourier-transform infrared (FT-IR) spectroscopy coupled with multivariate data analysis. The FT-IR spectra of 97 samples were obtained for wave numbers ranging from 4000 to 400 cm-1. Then, the fingerprint region of 1800-600 cm-1 of the FT-IR spectrum, rather than the full spectrum, was analyzed. Different pretreatments were applied on the spectra, and a discriminant analysis model based on the Mahalanobis distance was developed to select an optimal pretreatment combination...
2016: International Journal of Medicinal Mushrooms
https://www.readbyqxmd.com/read/27910681/tokamak-independent-software-analysis-suite-for-multi-spectral-line-polarization-mse-diagnostics
#4
S D Scott, R T Mumgaard
A tokamak-independent analysis suite has been developed to process data from Motional Stark Effect (mse) diagnostics. The software supports multi-spectral line-polarization mse diagnostics which simultaneously measure emission at the mse σ and π lines as well as at two "background" wavelengths that are displaced from the mse spectrum by a few nanometers. This analysis accurately estimates the amplitude of partially polarized background light at the σ and π wavelengths even in situations where the background light changes rapidly in time and space, a distinct improvement over traditional "time-interpolation" background estimation...
November 2016: Review of Scientific Instruments
https://www.readbyqxmd.com/read/27910657/time-dependent-analysis-of-visible-helium-line-ratios-for-electron-temperature-and-density-diagnostic-using-synthetic-simulations-on-nstx-u
#5
J M Muñoz Burgos, T Barbui, O Schmitz, D Stutman, K Tritz
Helium line-ratios for electron temperature (Te) and density (ne) plasma diagnostic in the Scrape-Off-Layer (SOL) and edge regions of tokamaks are widely used. Due to their intensities and proximity of wavelengths, the singlet, 667.8 and 728.1 nm, and triplet, 706.5 nm, visible lines have been typically preferred. Time-dependency of the triplet line (706.5 nm) has been previously analyzed in detail by including transient effects on line-ratios during gas-puff diagnostic applications. In this work, several line-ratio combinations within each of the two spin systems are analyzed with the purpose of eliminating transient effects to extend the application of this powerful diagnostic to high temporal resolution characterization of plasmas...
November 2016: Review of Scientific Instruments
https://www.readbyqxmd.com/read/27910653/new-type-of-wavelet-based-spectral-analysis-by-which-modes-with-different-toroidal-mode-number-are-separated
#6
S Ohdachi
A new type of wavelet-based analysis for the magnetic fluctuations by which toroidal mode number can be resolved is proposed. By using a wavelet, having a different phase toroidally, a spectrogram with a specific toroidal mode number can be obtained. When this analysis is applied to the measurement of the fluctuations observed in the large helical device, MHD activities having similar frequency in the laboratory frame can be separated from the difference of the toroidal mode number. It is useful for the non-stationary MHD activity...
November 2016: Review of Scientific Instruments
https://www.readbyqxmd.com/read/27910634/the-upgrade-of-the-thomson-scattering-system-for-measurement-on-the-c-2-c-2u-devices
#7
K Zhai, T Schindler, J Kinley, B Deng, M C Thompson
The C-2/C-2U Thomson scattering system has been substantially upgraded during the latter phase of C-2/C-2U program. A Rayleigh channel has been added to each of the three polychromators of the C-2/C-2U Thomson scattering system. Onsite spectral calibration has been applied to avoid the issue of different channel responses at different spots on the photomultiplier tube surface. With the added Rayleigh channel, the absolute intensity response of the system is calibrated with Rayleigh scattering in argon gas from 0...
November 2016: Review of Scientific Instruments
https://www.readbyqxmd.com/read/27910554/a-libs-method-for-simultaneous-monitoring-of-the-impurities-and-the-hydrogenic-composition-present-in-the-wall-of-the-tj-ii-stellarator
#8
B López-Miranda, B Zurro, A Baciero, M Martínez
The study of plasma-wall interactions and impurity transport in the plasma fusion devices is critical for the development of future fusion reactors. An experiment to perform laser induced breakdown spectroscopy, using minor modifications of our existing laser blow-off impurity injection system, has been set up thus making both experiments compatible. The radiation produced by the laser pulse focused at the TJ-II wall evaporates a surface layer of deposited impurities and the subsequent radiation produced by the laser-produced plasma is collected by two separate lens and fiber combinations into two spectrometers...
November 2016: Review of Scientific Instruments
https://www.readbyqxmd.com/read/27910500/development-of-visible-spectroscopy-diagnostics-for-w-sources-assessment-in-west
#9
O Meyer, O M Jones, J C Giacalone, J Y Pascal, D Raulin, H Xu, M H Aumeunier, R Baude, A Escarguel, C Gil, J H Harris, J-C Hatchressian, C C Klepper, S Larroque, Ph Lotte, Ph Moreau, B Pégourié, S Vartanian
The present work concerns the development of a W sources assessment system in the framework of the tungsten-W environment in steady state tokamak project that aims at equipping the existing Tore Supra device with a tungsten divertor in order to test actively cooled tungsten Plasma Facing Components (PFCs) in view of preparing ITER operation. The goal is to assess W sources and D recycling with spectral, spatial, and temporal resolution adapted to the PFCs observed. The originality of the system is that all optical elements are installed in the vacuum vessel and compatible with steady state operation...
November 2016: Review of Scientific Instruments
https://www.readbyqxmd.com/read/27910482/upgraded-divertor-thomson-scattering-system-on-diii-d
#10
F Glass, T N Carlstrom, D Du, A G McLean, D A Taussig, R L Boivin
A design to extend the unique divertor Thomson scattering system on DIII-D to allow measurements of electron temperature and density in high triangularity plasmas is presented. Access to this region is selectable on a shot-by-shot basis by redirecting the laser beam of the existing divertor Thomson system inboard - beneath the lower floor using a moveable, high-damage threshold, in-vacuum mirror - and then redirecting again vertically. The currently measured divertor region remains available with this mirror retracted...
November 2016: Review of Scientific Instruments
https://www.readbyqxmd.com/read/27910402/towards-10-mev-resolution-the-design-of-an-ultrahigh-resolution-soft-x-ray-rixs-spectrometer
#11
Joseph Dvorak, Ignace Jarrige, Valentina Bisogni, Scott Coburn, William Leonhardt
We present the optical design of the Centurion soft X-ray resonant inelastic X-ray scattering (RIXS) spectrometer to be located on the SIX beamline at NSLS-II. The spectrometer is designed to reach a resolving power of 100 000 at 1000 eV at its best resolution. It is also designed to have continuously variable 2θ motion over a range of 112° using a custom triple rotating flange. We have analyzed several possible spectrometer designs capable of reaching the target resolution. After careful analysis, we have adopted a Hettrick-Underwood spectrometer design, with an additional plane mirror to maintain a fixed direction for the outgoing beam...
November 2016: Review of Scientific Instruments
https://www.readbyqxmd.com/read/27910348/demonstration-of-imaging-x-ray-thomson-scattering-on-omega-ep
#12
Patrick X Belancourt, Wolfgang Theobald, Paul A Keiter, Tim J B Collins, Mark J Bonino, Pawel M Kozlowski, Sean P Regan, R Paul Drake
Foams are a common material for high-energy-density physics experiments because of low, tunable densities, and being machinable. Simulating these experiments can be difficult because the equation of state is largely unknown for shocked foams. The focus of this experiment was to develop an x-ray scattering platform for measuring the equation of state of shocked foams on OMEGA EP. The foam used in this experiment is resorcinol formaldehyde with an initial density of 0.34 g/cm(3). One long-pulse (10 ns) beam drives a shock into the foam, while the remaining three UV beams with a 2 ns square pulse irradiate a nickel foil to create the x-ray backlighter...
November 2016: Review of Scientific Instruments
https://www.readbyqxmd.com/read/27910174/combined-application-of-uhplc-qtof-ms-hplc-elsd-and-1-h-nmr-spectroscopy-for-quality-assessment-of-da-9801-a-standardised-dioscorea-extract
#13
Kyo Bin Kang, Jayoung Ryu, Youngwoong Cho, Sang-Zin Choi, Miwon Son, Sang Hyun Sung
INTRODUCTION: DA-9801, a standardised 50% aqueous ethanolic extract of a mixture of Dioscorea japonica and D. nipponica, is a botanical drug candidate for the treatment of diabetic neuropathy, which finished its US phase II clinical trials recently. An advanced quality control method is needed for further development of DA-9801, considering its high contents of both primary and secondary metabolites. OBJECTIVE: Development of a quality assessment strategy for DA-9801, based on the combination of UHPLC-QTOF/MS, HPLC-ELSD, and (1) H-NMR spectroscopy...
December 1, 2016: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/27910005/doppler-images-of-intra-pulmonary-shunt-within-atelectasis-in-anesthetized-children
#14
Cecilia M Acosta, Gerardo Tusman, Mauro Costantini, Camila Echevarría, Sergio Pollioto, Diego Abrego, Fernando Suarez-Sipmann, Stephan H Böhm
BACKGROUND: Doppler images of pulmonary vessels in pulmonary diseases associated with subpleural consolidations have been described. Color Doppler easily identifies such vessels within consolidations while spectral Doppler analysis allows the differentiation between pulmonary and bronchial arteries. Thus, Doppler helps in diagnosing the nature of consolidations. To our knowledge, Doppler analysis of pulmonary vessels within anesthesia-induced atelectasis has never been described before...
December 2016: Critical Ultrasound Journal
https://www.readbyqxmd.com/read/27909886/automatic-snoring-sounds-detection-from-sleep-sounds-via-multi-features-analysis
#15
Can Wang, Jianxin Peng, Lijuan Song, Xiaowen Zhang
Obstructive sleep apnea hypopnea syndrome (OSAHS) is a serious respiratory disorder. Snoring is the most intuitively characteristic symptom of OSAHS. Recently, many studies have attempted to develop snore analysis technology for diagnosing OSAHS. The preliminary and essential step in such diagnosis is to automatically segment snoring sounds from original sleep sounds. This study presents an automatic snoring detection algorithm that detects potential snoring episodes using an adaptive effective-value threshold method, linear and nonlinear feature extraction using maximum power ratio, sum of positive/negative amplitudes, 500 Hz power ratio, spectral entropy (SE) and sample entropy (SampEn), and automatic snore/nonsnore classification using a support vector machine...
December 1, 2016: Australasian Physical & Engineering Sciences in Medicine
https://www.readbyqxmd.com/read/27908089/vibration-analysis-of-coupled-conical-cylindrical-spherical-shells-using-a-fourier-spectral-element-method
#16
Zhu Su, Guoyong Jin
This paper presents a Fourier spectral element method (FSEM) to analyze the free vibration of conical-cylindrical-spherical shells with arbitrary boundary conditions. Cylindrical-conical and cylindrical-spherical shells as special cases are also considered. In this method, each fundamental shell component (i.e., cylindrical, conical, and spherical shells) is divided into appropriate elements. The variational principle in conjunction with first-order shear deformation shell theory is employed to model the shell elements...
November 2016: Journal of the Acoustical Society of America
https://www.readbyqxmd.com/read/27908053/detection-of-fricatives-using-s-transform
#17
Hari Krishna Vydana, Anil Kumar Vuppala
Two prime acoustic characteristics of fricatives are the concentration of spectral energy above 3 kHz and having noisy nature. Spectral domain approaches for detecting fricatives rely on capturing the information from spectral energy distribution. In this work, S-transform based time-frequency representation is explored for detecting fricatives from continuous speech. S-transform based time-frequency representation exhibits a progressive resolution which is tailored for localizing the high frequency events (i...
November 2016: Journal of the Acoustical Society of America
https://www.readbyqxmd.com/read/27907255/a-hyperspectral-image-can-predict-tropical-tree-growth-rates-in-single-species-stands
#18
T Trevor Caughlin, Sarah J Graves, Gregory P Asner, Michiel van Breugel, Jefferson S Hall, Roberta E Martin, Mark S Ashton, Stephanie A Bohlman
Remote sensing is increasingly needed to meet the critical demand for estimates of forest structure and composition at landscape to continental scales. Hyperspectral images can detect tree canopy properties, including species identity, leaf chemistry and disease. Tree growth rates are related to these measurable canopy properties but whether growth can be directly predicted from hyperspectral data remains unknown. We used a single hyperspectral image and light detection and ranging-derived elevation to predict growth rates for 20 tropical tree species planted in experimental plots...
December 2016: Ecological Applications: a Publication of the Ecological Society of America
https://www.readbyqxmd.com/read/27907139/contribution-towards-a-metabolite-profile-of-the-detoxification-of-benzoic-acid-through-glycine-conjugation-an-intervention-study
#19
Cindy Irwin, Mari van Reenen, Shayne Mason, Lodewyk J Mienie, Johan A Westerhuis, Carolus J Reinecke
Benzoic acid is widely used as a preservative in food products and is detoxified in humans through glycine conjugation. Different viewpoints prevail on the physiological significance of the glycine conjugation reaction and concerns have been raised on potential public health consequences following uncontrolled benzoic acid ingestion. We performed a metabolomics study which used commercial benzoic acid containing flavored water as vehicle for designed interventions, and report here on the controlled consumption of the benzoic acid by 21 cases across 6 time points for a total of 126 time points...
2016: PloS One
https://www.readbyqxmd.com/read/27906298/high-resolution-brillouin-optical-correlation-domain-analysis-with-no-spectral-scanning
#20
Eyal Preter, Dexin Ba, Yosef London, Orel Shlomi, Yair Antman, Avi Zadok
Distributed Brillouin fiber sensors typically rely on the reconstruction of the steady-state Brillouin gain spectrum (BGS), through spectral scanning of the frequency offset between the pump and signal waves. In this work, we propose and demonstrate an alternative approach, in which the local Brillouin frequency shift (BFS) is extracted from temporal transient analysis of the step response of the amplified signal wave. Measurements are taken at only two arbitrary frequency offsets between pump and signal. No spectral scanning and no prior knowledge of a reference BGS are necessary...
November 28, 2016: Optics Express
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