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https://www.readbyqxmd.com/read/29792802/the-anti-biofouling-properties-of-superhydrophobic-surfaces-are-short-lived
#1
Gi Byoung Hwang, Kristopher Page, Adnan Patir, Sean P Nair, Elaine Allan, Ivan P Parkin
Superhydrophobic surfaces are present in nature on the leaves of many plant species. Water rolls on these surfaces and the rolling motion picks up particles including bacteria and viruses. Man-made superhydrophobic surfaces have been made in an effort to reduce biofouling. We show here that the anti-biofouling property of a superhydrophobic surface is due to an entrapped air-bubble layer that reduces contact between the bacteria and the surface. Further, we show that prolonged immersion of superhydrophobic surfaces in water led to loss of the bubble-layer and subsequent bacterial adhesion that unexpectedly, exceeded that of the control materials...
May 24, 2018: ACS Nano
https://www.readbyqxmd.com/read/29789104/functionalized-caprolactone-polyethylene-glycol-based-thermo-responsive-hydrogels-of-silibinin-for-the-treatment-of-malignant-melanoma
#2
Behzad Sharif Makhmalzadeh, Ommoleila Molavi, Mohammad Reza Vakili, Hai-Feng Zhang, Amir Solimani, Hoda Soleymani Abyaneh, Raimar Loebenberg, Raymond Lai, Afsaneh Lavasanifar
PURPOSE: Silibinin, is a natural compound, which has shown anticancer activity in various malignancies. In this study, we evaluated the anticancer effects of silibinin in B16-F10 melanoma cells and developed a novel thermoresponsive hydrogel for local delivery of this compound. METHOD: A thermoresponsive hydrogel loaded with silibinin was prepared using triblock copolymers of poly[(α-benzyl carboxylate-e-caprolactone)-co-(α-carboxyl-e-caprolactone)]ran-b-PEG-b-[(α-benzyl carboxylate-e-caprolactone) -co-(α-carboxyl-e-caprolactone)]ran (PCBCL-b-PEG-b-PCBCL), namely PolyGelTM, and compared with a Pluronic F-127 formulation of silibinin...
2018: Journal of Pharmacy & Pharmaceutical Sciences: a Publication of the Canadian Society for Pharmaceutical Sciences
https://www.readbyqxmd.com/read/29789102/an-update-on-drug-induced-oral-reactions
#3
Hila Yousefi, Mohammas Abdollahi
Adverse drug reactions (ADRs) are one of the major culprits in the development of oral lesions, which can be misdiagnosed with underlying diseases. The goal of this study is to summarize and update the current knowledge about drug-induced oral reactions. Electronic searches were performed in Scopus, Google Scholar, Cochrane and PubMed databases, for articles published between January 2008 and August 2017. Two authors screened the titles and abstracts for eligibility. Finally, 56 studies included in this review...
2018: Journal of Pharmacy & Pharmaceutical Sciences: a Publication of the Canadian Society for Pharmaceutical Sciences
https://www.readbyqxmd.com/read/29789101/licofelone-attenuates-lps-induced-depressive-like-behavior-in-mice-a-possible-role-for-nitric-oxide
#4
Seyyedeh Elaheh Mousavi, Pegah Saberi, Naeemeh Ghasemkhani, Nahid Fakhraei, Rezvan Mokhtari, Ahmad Reza Dehpour
PURPOSE: Licofelone, a dual cyclooxygenase/5-lipoxygenase inhibitor, possesses antioxidant, antiapoptotic, neuroprotective, and anti-inflammatory properties. The aim of the present study was to investigate the effect of licofelone on lipopolysaccharide (LPS)-induced depression in a mouse model and also a possible role for nitric oxide (NO). METHODS: To elucidate the role of NO on this effect of licofelone (5 and 20 mg/kg, i.p.), L-NAME, a non-specific NO synthase (NOS) inhibitor; aminoguanidine (AG), a specific inducible NOS (iNOS) inhibitor; 7-nitroindazole (7-NI) a preferential neuronal NOS inhibitor (nNOS) and; L-arginine (L-Arg), as a NO donor, were used...
2018: Journal of Pharmacy & Pharmaceutical Sciences: a Publication of the Canadian Society for Pharmaceutical Sciences
https://www.readbyqxmd.com/read/29783967/characterization-of-a-novel-thermostable-and-xylose-tolerant-gh-39-%C3%AE-xylosidase-from-dictyoglomus-thermophilum
#5
Qi Li, Tao Wu, Zhipeng Qi, Linguo Zhao, Jianjun Pei, Feng Tang
BACKGROUND: β-D-xylosidase is a vital exoglycosidase with the ability to hydrolyze xylooligosaccharides to xylose and to biotransform some saponins by cleaving outer β-xylose. β-D-xylosidase is widely used as one of the xylanolytic enzymes in a diverse range of applications, such as fuel, food and the pharmaceutical industry; therefore, more and more studies have focused on the thermostable and xylose-tolerant β-D-xylosidases. RESULTS: A thermostable β-xylosidase gene (xln-DT) of 1509 bp was cloned from Dictyoglomus thermophilum and expressed in E...
May 21, 2018: BMC Biotechnology
https://www.readbyqxmd.com/read/29780325/proteomics-of-stored-red-blood-cell-membrane-and-storage-induced-microvesicles-reveals-the-association-of-flotillin-2-with-band-3-complexes
#6
Michel Prudent, Julien Delobel, Aurélie Hübner, Corinne Benay, Niels Lion, Jean-Daniel Tissot
The storage of erythrocyte concentrates (ECs) induces lesions that notably affect metabolism, protein activity, deformability of red blood cells (RBCs), as well as the release of oxygen. Band 3 is one of the proteins affected during the ex vivo aging of RBCs. This membrane protein is an anion transporter, an anchor site for the cytoskeleton and other membrane proteins as well as a binding site for glycolytic enzymes and bears blood group antigens. In the present study, band 3 complexes were isolated from RBCs stored for 7 and 42 days in average ( n = 3), as well as from microvesicles ( n = 3)...
2018: Frontiers in Physiology
https://www.readbyqxmd.com/read/29773322/polarization-of-the-vaccination-debate-on-facebook
#7
Ana Lucía Schmidt, Fabiana Zollo, Antonio Scala, Cornelia Betsch, Walter Quattrociocchi
BACKGROUND: Vaccine hesitancy has been recognized as a major global health threat. Having access to any type of information in social media has been suggested as a potential influence on the growth of anti-vaccination groups. Recent studies w.r.t. other topics than vaccination show that access to a wide amount of content through the Internet without intermediaries resolved into major segregation of the users in polarized groups. Users select information adhering to theirs system of beliefs and tend to ignore dissenting information...
May 14, 2018: Vaccine
https://www.readbyqxmd.com/read/29765994/heterologous-expression-purification-and-characterization-of-the-hspx-ppe44-and-esxv-proteins-of-mycobacterium-tuberculosis
#8
Yousef Amini, Mohsen Tafaghodi, Saeid Amel Jamehdar, Zahra Meshkat, Bagher Moradi, Mojtaba Sankian
Background: Subunit vaccines are appropriate vaccine candidates for the prevention of some infections. In this study, three immunogenic proteins of Mycobacterium tuberculosis , including HspX, Ppe44, and EsxV as a new construction, were expressed alone and as a fusion protein to develop a new vaccine candidate against tuberculosis infection. Methods: To make the fusion protein, the three genes were linked together by AEAAAKEAAAKA linkers and inserted into pET21b and pET32b vectors...
April 2018: Reports of Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29757586/production-of-recombinant-protein-of-salsola-kali-sal-k1-pollen-allergen-in-lactococcus-lactis
#9
Ziba Ghasemi, Abdol-Reza Varasteh, Malihe Moghadam, Farnaz Sedghi, Farahzad Jabbariazad, Mojtaba Sankian
The Salsola kali pollen is considered the main cause of allergic sensitization in desert and semi-desert regions. We have constructed recombinant Lactococcus lactis producing Sal k1 protein with the aim of using it as a mucosal vaccine for specific immunotherapy. The Sal k1 gene was amplified, and transferred into a PNZ 8148 plasmid. The PNZ8148-Sal k1 recombinant plasmid was transformed into competent E.coli strain MC1061 for replication, and then was isolated and cloned into competent L. lactis by electroporation...
April 2018: Iranian Journal of Allergy, Asthma, and Immunology
https://www.readbyqxmd.com/read/29744278/proteomic-identification-of-marker-proteins-and-its-application-to-authenticate-ophiocordyceps-sinensis
#10
Xinxin Tong, Yixuan Wang, Zhengyao Xue, Lu Chen, Yi Qiu, Jing Cao, Cheng Peng, Jinlin Guo
Ophi o cordyceps sinensis ( O. sinensis ) is a highly valuable fungus because of its nutritious and medicinal properties. The objective of this study was to identify protein markers using a proteomics approach followed by the development of an immunoassay to authenticate O. sinensis . Four authentic O. sinensis samples collected from four production regions and four counterfeit samples were examined individually. Overall 22 characteristic proteins of O. sinensis were identified by two-dimensional electrophoresis (2-DE) coupled with the matrix-assisted laser desorption/ionization-time-of-light mass spectrometry (MALDI-TOF/MS)...
May 2018: 3 Biotech
https://www.readbyqxmd.com/read/29731402/-how-can-we-explain-the-crimes-of-nazis-doctors-in-auschwitz
#11
Bruno Halioua
The Nazi doctors committed crimes in the camp of Auschwitz which constitute the darkest page in the history of medicine of the twentieth century. They were largely directly involved in the murder of the 1.1 million Jewish majority and about 21,000 gypsies who were transported from all over Europe. How can we explain the intellectual journey of those men who had embraced the medical career to relieve their neighbors? How could these cultivated and refined men, from the most prestigious faculties of medicine, have been able to achieve activities ethically unacceptable? Eight factors help to explain the crimes committed by these educated and cultivated SS doctors: indoctrination fervent to Nazism, adherence to racial hygiene, a false sense of participation in a "public health enterprise", virulent anti-Semitism, unreserved obedience to orders, the opportunity to avoid the danger of fighting areas, the ambition to carry out medical research and the desire to improve surgical knowledge...
May 3, 2018: La Presse Médicale
https://www.readbyqxmd.com/read/29729069/ectoine-a-compatible-solute-in-radio-halophilic-stenotrophomonas-sp-wma-lm19-strain-to-prevent-ultraviolet-induced-protein-damage
#12
Wasim Sajjad, Sundas Qadir, Manzoor Ahmad, Muhammad Rafiq, Fariha Hasan, Richard Tehan, Kerry L McPhail, Aamer Ali Shah
AIM: The current study was conducted to investigate the possible role of a compatible solute from radio-halophilic bacterium against desiccation and ultra-violet radiation induced oxidative stress. METHODS AND RESULTS: Nine different radio-resistant bacteria were isolated from desert soil, where strain WMA-LM19 was chosen for detailed studies on the basis of its high tolerance to ultraviolet radiation among all these isolates. 16S rRNA gene sequencing indicated the bacterium was closely related to Stenotrophomonas sp...
May 4, 2018: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29720833/evidence-for-the-involvement-of-cox-2-vegf-and-pten-pl3k-akt-pathway-the-mechanism-of-oroxin-b-treated-liver-cancer
#13
Nan-Nan Li, Xian-Sheng Meng, Yong-Rui Bao, Shuai Wang, Tian-Jiao Li
Background: Oroxin B (OB) is one of flavonoids isolated from traditional Chinese herbal medicine Oroxylum indicum (L.) Vent. Recent studies suggest that flavonoids have obvious anti-liver tumors effect, but the precise molecular mechanism is still unclear. Objective: The current study was performed to investigate the antitumor effects of OB on human hepatoma cell line SMMC-772 and explore the part of molecular mechanisms in this process. Materials and Methods: MTT method, terminal deoxynucleotidyl transferase dUTP nick end labeling assay and flow cytometry were utilized to detect the inhibition of proliferation and the apoptosis after treating OB in of SMMC-7721 cells...
April 2018: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/29701030/-preparation-of-anti-hcg-single-domain-antibody-by-antibody-grafting-technique-using-an-antigen-binding-peptide
#14
Jing Peng, Qiong Wang, Xiaoling Cheng, Mengwen Liu, Mei Wang, Huawei Xin
We used the antibody grafting technology to prepare anti-hCG single-domain antibodies on the basis of antigen-binding peptide to simplify the single-domain antibody preparation process and improving the biochemical stability of peptide. By using a universal single-domain antibody backbone (cAbBCII10), CDR1 or CDR3 was replaced by the hCG-binding peptide, and the grafted antibody gene sequences were synthesized and cloned into the prokaryotic expression vector pET30a(+) in fusion with a C-terminal sfGFP gene, i...
April 25, 2018: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
https://www.readbyqxmd.com/read/29698702/a-dimeric-form-of-a-small-sized-protein-binder-exhibits-enhanced-anti-tumor-activity-through-prolonged-blood-circulation
#15
Tae Yoon Kim, Hyo-Deok Seo, Joong-Jae Lee, Jung Ae Kang, Woo Sik Kim, Hye-Min Kim, Ha-Yeon Song, Ji Min Park, Dong-Eun Lee, Hak-Sung Kim
Small-sized non-antibody scaffolds have attracted considerable interest as alternatives to immunoglobulin antibodies. However, their short half-life is considered a drawback in the development of therapeutic agents. Here we demonstrate that a homo-dimeric form of a repebody enhances the anti-tumor activity than a monomeric form through prolonged blood circulation. Spytag and spycatcher were genetically fused to the C-terminus of a respective human IL-6-specific repebody, and the resulting two repebody constructs were mixed at an equimolar ratio to produce a homo-dimeric form through interaction between spytag and spycatcher...
April 23, 2018: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/29696080/therapeutic-effects-of-serum-extracellular-vesicles-in-liver-fibrosis
#16
Li Chen, Ruju Chen, Sherri Kemper, Min Cong, Hong You, David R Brigstock
The lack of approved therapies for hepatic fibrosis seriously limits medical management of patients with chronic liver disease. Since extracellular vesicles (EVs) function as conduits for intercellular molecular transfer, we investigated if EVs from healthy individuals have anti-fibrotic properties. Hepatic fibrogenesis or fibrosis in carbon tetrachloride (CCl4 )- or thioacetic acid-induced liver injury models in male or female mice were suppressed by serum EVs from normal mice (EVN) but not from fibrotic mice (EVF)...
2018: Journal of Extracellular Vesicles
https://www.readbyqxmd.com/read/29689714/production-and-characterization-of-anti-human-igg-f-ab-2-antibody-fragment
#17
Zahra Valedkarimi, Hadi Nasiri, Leili Aghebati-Maleki, Jalal Abdolalizadeh, Mojghan Esparvarinha, Jafar Majidi
In present study an optimized protocol for the separation of antibodies into antigen-binding fragments F(ab')2 using pepsin digestion was investigated. The production of these fragments is a consequential step in the development of medical research, treatment and diagnosis. For production of polyclonal antibody rabbit received antigen in four steps. The rabbit serum at 1/128000 dilution showed high absorbance in reaction with human IgG at the designed ELISA method. Rabbit IgG was purified by Ion-Exchange Chromatography (IEC) method...
April 10, 2018: Human Antibodies
https://www.readbyqxmd.com/read/29688063/evaluation-of-a-novel-biocompatible-magnetic-nanomedicine-based-on-beta-cyclodextrin-loaded-doxorubicin-curcumin-for-overcoming-chemoresistance-in-breast-cancer
#18
Roghayeh Rastegar, Hamid Akbari Javar, Mehdi Khoobi, Poua Dehghan Kelishadi, Gholam Hossein Yousefi, Mahmoud Doosti, Mohammad Hossien Ghahremani, Ahmad Sharif Tabrizi, Fatemeh Imanparast, Elham Gholibeglu, Mahdi Gholami
Codelivery of chemo-sensitizers with chemotherapeutics using combo nanomedicine is a promising platform for overcoming chemoresistance in breast cancer. However, tumor accumulation of nano-carriers based on enhanced permeability and retention (EPR) effect is confounded by heterogeneity in tumor microenvironment. Adsorption of protein corona on surface of nanoparticle boost up clearance by reticulo-endothelial system. In this study, a surface functionalized magnetic nanocomposite (NC) for codelivery of doxorubicin (DOX) and curcumin (CUR) is developed...
April 24, 2018: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/29665935/-prokaryotic-expression-and-purification-of-the-notch-ligand-and-its-effect-on-hematopoiesis-after-carbon-tetrachloride-damage
#19
Juan-Juan Chen, Si-Yong Huang, Peng-Fei Ma, Bi-Jia Wu, Si-Lang Zhou, Yong-Xing Zhao, Jun-Mei Gong, Ying-Min Liang
OBJECTIVE: To express and purify the mouse endothelial cell-targeted recombinant Notch ligand protein mD1R, and to investigate its effect on hematopoiesis after carbon tetrachloride damage. METHODS: PCR was performed to clone and construct the expression vector pET22b(+)-mD1R. The mD1R successfully transformed into E. coli was induced by IPTG, and purified with Ni2+ -beads affinity chromatography. The target protein was detected by SDS-PAGE. The fluorescence-activated cell sorting analysis (FACS), cell adhesion test, immunofluorescence staining and quantitative real-time PCR were employed to detect the endothelial cell-targeted and Notch signaling-activated biological characteristics of mD1R...
April 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://www.readbyqxmd.com/read/29626520/efficient-protease-based-purification-of-recombinant-matrix-metalloprotease-1-in-e-coli
#20
Lokender Kumar, Warren Colomb, John Czerski, Christopher R Cox, Susanta K Sarkar
MMP1 is an essential enzyme for tissue remodeling both in normal and pathological states. We report a method of purifying activated human MMP1 in E. coli without using urea or 4-Aminophenylmercuric acetate (APMA). Instead, a non-ionic detergent, Triton X-100, was used in the lysis buffer to solubilize MMP1 followed by the protease activities of both trypsin and MMP1 to digest E. coli proteins and activate pro-MMP1. Identity of activated MMP1 was confirmed by Western blot using anti-human MMP1 antibodies, whereas the mass was determined to be 43 kD using matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI TOF-MS)...
April 4, 2018: Protein Expression and Purification
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