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https://www.readbyqxmd.com/read/29162063/elevated-aspergillus-specific-antibody-levels-among-hiv-infected-ugandans-with-pulmonary-tuberculosis
#1
Richard Kwizera, Rosalind Parkes-Ratanshi, Iain D Page, Christine Sekaggya-Wiltshire, Joseph Musaazi, Jan Fehr, Barbara Castelnuovo, Andrew Kambugu, David W Denning
BACKGROUND: The incidence of tuberculosis (TB) is high among human immunodeficiency virus (HIV) infected Ugandans. Recent evidence suggests that Chronic Pulmonary Aspergillosis and Aspergillus sensitisation might be responsible for significant mortality in patients treated for tuberculosis in Uganda. METHODS: We retrieved and tested paired serum aliquots for 101 HIV-TB co-infected patients at the beginning and week 24 of TB treatment. We tested samples for Aspergillus-specific immunoglobulin G (IgG) and immunoglobulin E (IgE) using ImmunoCAP®; and Aspergillus-specific IgG and total serum IgE using Immulite® immunoassays...
November 21, 2017: BMC Pulmonary Medicine
https://www.readbyqxmd.com/read/29158139/expression-and-evaluation-of-recombinant-p32-protein-based-elisa-for-sero-diagnostic-potential-of-capripox-in-sheep-and-goats
#2
Gnanavel Venkatesan, Mahesh Kumar Teli, Muthu Sankar, Amit Kumar, M Dashprakash, Sargam Arya, Aparna Madhavan, Muthannan Andavar Ramakrisnan, Awadh Bihari Pandey
The study is aimed to develop and evaluate a recombinant P32 protein based ELISA for sero-monitoring and sero-surveillance using known and suspected serum samples for capripox from sheep and goats. Truncated P32 gene of goatpox virus (with an ORF of 750 bp) was expressed in E. coli BL-21 CodonPlus (DE3) cells using pET32a vector and characterized by SDS-PAGE analysis and confirmed by western blotting as 48 KDa Poly-His tagged fusion protein. The protein was purified under denaturing conditions using 8M urea and characterized by SDS-PAGE and immunoblotting...
November 17, 2017: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/29144770/equine-intestinal-mucosal-pathobiology
#3
Anthony Blikslager, Liara Gonzalez
The equine intestinal mucosa is intimately involved in maintaining homeostasis both on a systemic level by controlling extracellular fluid movement and at the local level to maintain barrier function. Horses are particularly susceptible to the clinical syndrome of colic, with the most severe cases involving strangulating obstruction that induces ischemia. Because of the mucosal vascular architecture, the mucosal epithelium is particularly susceptible to ischemic injury. The potential for reperfusion injury has been investigated and found to play a minimal role...
November 16, 2017: Annual Review of Animal Biosciences
https://www.readbyqxmd.com/read/29143311/interleukin-12-and-its-procoagulant-effect-on-erythrocytes-platelets-and-fibrin-ogen-the-lesser-known-side-of-inflammation
#4
Martin J Page, Janette Bester, Etheresia Pretorius
Inflammation, with its associated inflammatory molecules, is integral to most chronic diseases, including the various cardiovascular diseases. Interleukin 12 (IL12) is one of the inflammatory cytokines that is upregulated during inflammation; however, we know very little about its exact effect on red blood cells (RBCs), platelets and fibrin(ogen). IL12 is an important pleiotropic cytokine in early inflammatory responses and has potent immunomodulatory, antitumour and anti-infection activity. Here we investigate how low levels of circulating IL12, comparable to levels found during chronic inflammation, affect coagulation parameters, platelets and RBCs...
November 16, 2017: British Journal of Haematology
https://www.readbyqxmd.com/read/29141539/comparative-selectively-targeted-anti-neoplastic-cytotoxicity-of-three-immunopharmaceuticals-with-covalently-bound-fludarabine-gemcitabine-and-dexamethasone-moieties-synthesized-utilizing-organic-chemistry-reactions-in-a-multi-phase-regimen
#5
Cody P Coyne, Lakshmi Narayanan
Background - Unintentional passive diffusion of conventional small molecular weight pharmaceuticals across intact membranes of normal healthy cells in tissues and organ systems induces sequelae that limit therapeutic dosage and duration of administration. Selective "targeted" delivery of pharmaceuticals is a molecular strategy that can potentially provide heightened margins-of-safety with greater potency and improved efficacy. Materials-and-Methods - Monophosphate analogs of fludarabine, gemcitabine, and dexamethasone were combined with a carbodiimide reagent in the presence of imidazole to produce reactive intermediates that were subsequently covalently bound to monoclonal anti-IGF-1R or anti-EGFR IgG-immunoglobulin...
November 14, 2017: Current Pharmaceutical Design
https://www.readbyqxmd.com/read/29132905/purification-and-biochemical-characterization-of-a-22-kda-stable-cysteine-like-protease-from-the-excretory-secretory-product-of-the-liver-fluke-fasciola-hepatica-by-using-conventional-techniques
#6
Ahmed Hemici, Roumaila Sabrina Benerbaiha, Dalila Bendjeddou
This study describes the purification and characterization of a stable protease activity isolated from Fasciola hepatica adult worms maintained in vitro by employing acetone precipitation (40-60%) followed by a gel filtration through Sephadex G-100 and DEAE- cellulose ion exchange column. Through this three-step purification, the enzyme was purified 11-fold with a specific activity of 1893.9U/mg and 31.5% recovery. After the final ultrafiltration step, the purification fold was increased up to 13.1 and the overall activity yield reached a rate of 18...
October 28, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29128668/inhibition-of-the-complement-system-by-saliva-of-anopheles-nyssorhynchus-aquasalis
#7
Antonio Ferreira Mendes-Sousa, Vladimir Fazito Vale, Daniel Costa Queiroz, Adalberto Alves Pereira-Filho, Naylene Carvalho Sales da Silva, Leonardo Barbosa Koerich, Luciano Andrade Moreira, Marcos Horácio Pereira, Maurício Roberto Sant'Anna, Ricardo Nascimento Araújo, John Andersen, Jesus Gilberto Valenzuela, Nelder Figueiredo Gontijo
Anopheline mosquitoes are vectors of malaria parasites. Their saliva contains anti-hemostatic and immune-modulator molecules that favor blood feeding and parasite transmission. In this study, we describe the inhibition of the alternative pathway of the complement system (AP) by Anopheles aquasalis salivary gland extracts (SGE). According to our results, the inhibitor present in SGE acts on the initial step of the AP blocking deposition of C3b on the activation surfaces. Properdin, which is a positive regulatory molecule of the AP, binds to SGE...
November 8, 2017: Insect Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/29121987/sequence-analysis-and-characterization-of-pyruvate-kinase-from-clonorchis-sinensis-a-53-1-kda-homopentamer-implicated-immune-protective-efficacy-against-clonorchiasis
#8
Tingjin Chen, Hongye Jiang, Hengchang Sun, Zhizhi Xie, Pengli Ren, Lu Zhao, Huimin Dong, Mengchen Shi, Zhiyue Lv, Zhongdao Wu, Xuerong Li, Xinbing Yu, Yan Huang, Jin Xu
BACKGROUND: Clonorchis sinensis, the causative agent of clonorchiasis, is classified as one of the most neglected tropical diseases and affects more than 15 million people globally. This hepatobiliary disease is highly associated with cholangiocarcinoma. As key molecules in the infectivity and subsistence of trematodes, glycolytic enzymes have been targets for drug and vaccine development. Clonorchis sinensis pyruvate kinase (CsPK), a crucial glycolytic enzyme, was characterized in this research...
November 9, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/29121973/in-vitro-investigations-on-extracellular-proteins-secreted-by-aphanomyces-invadans-the-causative-agent-of-epizootic-ulcerative-syndrome
#9
Muhammad Majeed, Gokhlesh Kumar, Sarah Schlosser, Mansour El-Matbouli, Mona Saleh
BACKGROUND: Proteases produced by many microorganisms, including oomycetes, are crucial for their growth and development. They may also play a critical role in disease manifestation. Epizootic ulcerative syndrome is one of the most destructive fish diseases known. It is caused by the oomycete Aphanomyces invadans and leads to mass mortalities of cultured and wild fish in many countries. The areas of concern are Australia, China, Japan, South and Southeast Asian countries and the USA. Extracellular proteases produced by this oomycete are believed to trigger EUS pathogenesis in fish...
November 9, 2017: Acta Veterinaria Scandinavica
https://www.readbyqxmd.com/read/29111348/nrpa-308-a-new-neuropilin-1-antagonist-exerts-in%C3%A2-vitro-anti-angiogenic-and-anti-proliferative-effects-and-in%C3%A2-vivo-anti-cancer%C3%A2-effects-in-a-mouse-xenograft-model
#10
Wang-Qing Liu, Yves Lepelletier, Matthieu Montès, Lucia Borriello, Rafika Jarray, Renaud Grépin, Bertrand Leforban, Ali Loukaci, Rachid Benhida, Olivier Hermine, Sylvie Dufour, Gilles Pagès, Christiane Garbay, Françoise Raynaud, Reda Hadj-Slimane, Luc Demange
Neuropilin-1 (NRP-1) is an extra-cellular receptor for the main Vascular Endothelial Growth Factor over-expressed in tumour tissues, VEGF-A165. Consequently, NRP-1 is involved in angiogenesis and in tumour growth, and its over-expression is related to a clinical poor prognosis. NRP-1 appears as a major target in oncology, which remains poorly exploited. Herein, we report a new series of 18 small-sized fully organic VEGF-A165/NRP-1 antagonists (NRPas). These compounds share an original scaffold, including two linkers (sulphonamide and amide) and three aromatic cores...
October 28, 2017: Cancer Letters
https://www.readbyqxmd.com/read/29107082/purification-and-characterization-of-an-anti-hemorrhagic-protein-from-naja-naja-indian-cobra-venom
#11
K N Suvilesh, M Yariswamy, M N Savitha, Vikram Joshi, A N Nanjaraj Urs, Amog P Urs, M Choudhury, D Velmurugan, B S Vishwanath
Snake venom Kunitz-type proteins are well known to inhibit serine proteases but a few studies have also shown matrix metalloproteases (MMPs) inhibition. In view of the fact that MMPs and snake venom metalloproteases (SVMPs) have similar catalytic site, inhibition of SVMP activity by Kunitz-type proteins remains to be studied. Recent proteomic studies of Naja naja (N. naja) venom revealed the abundance of Kunitz-type proteins. In this regard, present study aimed at purification of a protease inhibitor from N...
October 26, 2017: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/29098723/establishing-a-novel-pig-a-gene-mutation-assay-in-l5178ytk-mouse-lymphoma-cells
#12
Yiying Wang, Javier Revollo, Page McKinzie, Mason G Pearce, Azra Dad, Berran Yucesoy, Hans Rosenfeldt, Robert H Heflich, Vasily N Dobrovolsky
The X-linked Pig-a gene encodes an enzyme required for the biosynthesis of glycosyl phosphatidylinositol (GPI) anchors. Pig-a mutant cells fail to synthesize GPI and to express GPI-anchored protein markers (e.g., CD90) on their surface. Marker deficiency serves as a phenotypic indicator of Pig-a mutation in various in vivo assays. Here, we describe an in vitro Pig-a mutation assay in L5178YTk(+/-) mouse lymphoma cells, in which mutant-phenotype cells are measured by flow cytometry using a fluorescent anti-CD90 antibody...
November 2, 2017: Environmental and Molecular Mutagenesis
https://www.readbyqxmd.com/read/29090225/mycobacterium-tuberculosis-hspx-esxs-fusion-protein-gene-cloning-protein-expression-and-purification-in-escherichia-coli
#13
Farzad Khademi, Arshid Yousefi-Avarvand, Mohammad Derakhshan, Zahra Meshkat, Mohsen Tafaghodi, Kiarash Ghazvini, Ehsan Aryan, Mojtaba Sankian
BACKGROUND: The purpose of this study was to clone, express, and purify a novel multidomain fusion protein of Micobacterium tuberculosis (Mtb) in a prokaryotic system. METHODS: An hspX/esxS gene construct was synthesized and ligated into a pGH plasmid, E. coli TOP10 cells were transformed, and the vector was purified. The vector containing the construct and pET-21b (+) plasmid were digested with the same enzymes and the construct was ligated into pET-21b (+). The accuracy of cloning was confirmed by colony PCR and sequencing...
October 2017: Reports of Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29046506/analysis-of-differentially-expressed-proteins-in-muscovy-duck-embryo-fibroblasts-infected-with-virulent-and-attenuated-muscovy-duck-reovirus-by-two-dimensional-polyacrylamide-gel-electrophoresis
#14
Mei-Qing Huang, Xiao-Xia Cheng, Shi-Long Chen, Min Zheng, Shao-Ying Chen
Muscovy duck reovirus (MDRV) belongs to the Orthoreovirus genus of the Reoviridae family, which is a significant poultry pathogen leading to high morbidity and mortality in ducklings. However, the pathogenesis of the virus is not well understood. In the present study, two-dimensional (2D) polyacrylamide gel electrophoresis (PAGE) combined with LC-MS-MS was used to identify differentially expressed proteins between Muscovy duck embryo fibroblasts (MDEF) infected with virulent (MV9710 strain) and attenuated (CA strain) MDRV and non-infected MDEFs...
October 18, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29036807/obtaining-and-characterization-of-monoclonal-antibodies-against-recombinant-extracellular-domain-of-human-epidermal-growth-factor-receptor-2
#15
Sarina Nurgul, Abeldenov Sailau, Turgimbayeva Aigerim, Zhylkibayev Assylbek, Ramankulov Erlan, Khassenov Bekbolat, Eskendirova Saule
Human epidermal growth factor receptor 2 (HER2) is an important biomarker for detection and treatment of different types of cancers such as breast, ovarian, stomach cancer. In this study, we developed a monoclonal antibody against the extracellular domain (ECD) of HER2 biomarker of breast cancer. For this purpose, the ECD-HER2 gene was amplified and cloned into an expression vector. Gene was generated in Escherichia coli BL21 (DE3) strain for expression of recombinant protein. The expressed protein was separated by SDS-PAGE and detected by anti-his monoclonal antibody in immunoblotting...
September 8, 2017: Human Antibodies
https://www.readbyqxmd.com/read/29031651/vaccine-criticism-presence-and-arguments-on-french-speaking-websites
#16
A Nugier, F Limousi, N Lydié
OBJECTIVE: To evaluate the presence of vaccine criticism on the French Web and to analyze strategies and arguments used by opponents of vaccination. METHODS: The most frequently used keywords associated with the terms "vaccination" and "vaccine" on Google.fr in September 2013 were identified and searched for individually on Google.fr. The links presented in the first three pages of results were reviewed to identify the most frequent providers of information. The proportion of critical content was determined by website type and a content analysis was performed...
October 11, 2017: Médecine et Maladies Infectieuses
https://www.readbyqxmd.com/read/29031617/steroid-sparing-effects-of-doxofylline
#17
Yanira Riffo-Vasquez, Radhakrishnan Venkatasamy, Clive P Page
Glucocorticosteroids are widely used in the treatment of asthma and chronic obstructive pulmonary disease (COPD). However, there are growing concerns about the side effect profile of this class of drug, particularly an increased risk of pneumonia. Over the last two decades there have been many attempts to find drugs to allow a reduction of glucocorticosteroids, including xanthines such as theophylline. Use of xanthines has been shown to lead to a reduction in the requirement for glucocorticosteroids, although xanthines also have a narrow therapeutic window limiting their wider use...
October 12, 2017: Pulmonary Pharmacology & Therapeutics
https://www.readbyqxmd.com/read/29029583/new-generation-high-potency-and-designer-antibodies-role-in-hiv-1-treatment
#18
Lucio Gama, Richard A Koup
Broadly neutralizing antibodies (bNAbs) have been evaluated as promising agents in the fight against infectious diseases. HIV-1-specific bNAbs, in particular, have been tested in both preventive and therapeutic modalities. Multiple bNAbs have been isolated, characterized, and assessed in vitro and in vivo, but no single antibody appears to possess the breadth and potency that may be needed if it is to be used in the treatment of HIV-1 infection. With the technological advances of the past decades, novel and more effective bNAbs have been identified or engineered for higher neutralizing potency, greater breadth, and increased serum half-life...
October 13, 2017: Annual Review of Medicine
https://www.readbyqxmd.com/read/29029582/new-concepts-in-the-treatment-of-malignant-pleural-mesothelioma
#19
Ori Wald, David J Sugarbaker
Malignant pleural mesothelioma (MPM) is a highly aggressive and generally incurable cancer. Current anti-MPM chemotherapy-based treatments are only marginally effective, and long-term survival remains an unmet goal. Nonetheless, in selected cases, personalized surgery-based multimodality treatments (MMT) have been shown to significantly extend survival. The design of MMT and selection of patients are challenging, and optimal results require accurate presurgical diagnosis, staging, and risk stratification. Further, meticulous surgical techniques and advanced radiation protocols must be applied...
October 13, 2017: Annual Review of Medicine
https://www.readbyqxmd.com/read/29026848/drug-induced-sialorrhea-and-microfluidic-chip-electrophoretic-analysis-of-engorged-adult-female-tick-saliva-of-haemaphysalis-longicornis-acari-ixodidae
#20
Mohammad Saiful Islam, Myung Jo You
BACKGROUND: The aim of the present study was to induce salivation in Haemaphysalis longicornis to increase saliva production and to characterize the collection of proteins present in the collected saliva using on-chip-electrophoresis. METHODS: Saliva of adult female engorged H. longicornis was collected by treatment with 0.2% dopamine hydrochloride. All protein samples were characterized by SDS-PAGE electrophoresis using a microfluidic High Sensitivity Protein Assay 250 kit by 2100 Bioanalyzer (Agilent Technologies, USA) under non-reducing conditions...
March 2017: Journal of Arthropod-borne Diseases
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