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https://www.readbyqxmd.com/read/28325600/synthesis-and-anti-cancer-activities-of-new-sulfonamides-4-substituted-triazolyl-nucleosides
#1
Soukaina Alaoui, Maeva Dufies, Mohsine Driowya, Luc Demange, Khalid Bougrin, Guillaume Robert, Patrick Auberger, Gilles Pagès, Rachid Benhida
Nucleoside analogues are among the most known drugs commonly used in antiviral and anticancer chemotherapies. Among them, those featuring a five-membered ring nucleobase are of utmost interest such as the anti-cancer agent AICAR or the anti-viral drug ribavirin. Despite its low activity in vitro in different cell lines, AICAR is under clinical development for several pathologies, thanks to its original mode of action. Indeed, AICAR induced autophagy cell death and is able, following this mechanism, to circumvent resistance to apoptotic drugs including kinase inhibitors currently on the market...
March 9, 2017: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/28323242/investigation-of-the-indigenous-fungal-community-populating-barley-grains-secretomes-and-xylanolytic-potential
#2
Abida Sultan, Jens C Frisvad, Birgit Andersen, Birte Svensson, Christine Finnie
The indigenous fungal species populating cereal grains produce numerous plant cell wall-degrading enzymes including xylanases, which could play important role in plant-pathogen interactions and in adaptation of the fungi to varying carbon sources. To gain more insight into the grain surface-associated enzyme activity, members of the populating fungal community were isolated, and their secretomes and xylanolytic activities assessed. Twenty-seven different fungal species were isolated from grains of six barley cultivars over different harvest years and growing sites...
March 17, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28317175/occupational-respiratory-allergy-in-peach-crop-workers
#3
Remedios Pérez-Calderón, M Ángeles Gonzalo-Garijo, Francisco José Rodríguez-Velasco, Silvia Sánchez-Vega, Borja Bartolomé-Zavala
BACKGROUND: Occupational respiratory diseases in workers of peach tree crops have been reported punctually and have been associated with sensitization to proteins present in both pollen and leaf tree. We report the study of 37 workers with respiratory symptoms related to occupational exposure to peach trees. METHODS: Prick tests and specific IgE determinations were performed with extracts from leaves and branches of peach tree. Immunodetection in leaf extract was realized by SDS-PAGE-immunoblotting with patient sera and rabbit serum anti-Pru p 3...
March 20, 2017: Allergy
https://www.readbyqxmd.com/read/28286754/prokaryotic-expression-of-%C3%AE-13-giardin-gene-and-its-intracellular-localization-in-giardia-lamblia
#4
Xingang Yu, Auwalu Yusuf Abdullahi, Sheng Wu, Weida Pan, Xianli Shi, Wei Hu, Liping Tan, Kangxin Li, Zhen Wang, Guoqing Li
To study prokaryotic expression and subcellular localization of α-13 giardin in Giardia lamblia trophozoites, α-13 giardin gene was amplified and cloned into prokaryotic expression vector pET-28a(+). The positive recombinant plasmid was transformed into E. coli BL21(DE3) for expression by using IPTG and autoinduction expression system (ZYM-5052). The target protein was validated by SDS-PAGE and Western blotting and purified by Ni-NTA Resin. Rabbits were immunized with purified fusion proteins for preparation of polyclonal antibody; then the intracellular location of α-13 giardin was determined by fluorescence immunoassay...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28278244/structure-of-salo-a-leishmaniasis-vaccine-candidate-from-the-sand-fly-lutzomyia-longipalpis
#5
Oluwatoyin A Asojo, Alan Kelleher, Zhuyun Liu, Jeroen Pollet, Elissa M Hudspeth, Wanderson C Rezende, Mallory Jo Groen, Christopher A Seid, Maha Abdeladhim, Shannon Townsend, Waldione de Castro, Antonio Mendes-Sousa, Daniella Castanheira Bartholomeu, Ricardo Toshio Fujiwara, Maria Elena Bottazzi, Peter J Hotez, Bin Zhan, Fabiano Oliveira, Shaden Kamhawi, Jesus G Valenzuela
BACKGROUND: Immunity to the sand fly salivary protein SALO (Salivary Anticomplement of Lutzomyia longipalpis) protected hamsters against Leishmania infantum and L. braziliensis infection and, more recently, a vaccine combination of a genetically modified Leishmania with SALO conferred strong protection against L. donovani infection. Because of the importance of SALO as a potential component of a leishmaniasis vaccine, a plan to produce this recombinant protein for future scale manufacturing as well as knowledge of its structural characteristics are needed to move SALO forward for the clinical path...
March 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28260979/a-measurement-of-the-calorimeter-response-to-single-hadrons-and-determination-of-the-jet-energy-scale-uncertainty-using-lhc-run-1-pp-collision-data-with-the-atlas-detector
#6
M Aaboud, G Aad, B Abbott, J Abdallah, O Abdinov, B Abeloos, R Aben, O S AbouZeid, N L Abraham, H Abramowicz, H Abreu, R Abreu, Y Abulaiti, B S Acharya, L Adamczyk, D L Adams, J Adelman, S Adomeit, T Adye, A A Affolder, T Agatonovic-Jovin, J Agricola, J A Aguilar-Saavedra, S P Ahlen, F Ahmadov, G Aielli, H Akerstedt, T P A Åkesson, A V Akimov, G L Alberghi, J Albert, S Albrand, M J Alconada Verzini, M Aleksa, I N Aleksandrov, C Alexa, G Alexander, T Alexopoulos, M Alhroob, B Ali, M Aliev, G Alimonti, J Alison, S P Alkire, B M M Allbrooke, B W Allen, P P Allport, A Aloisio, A Alonso, F Alonso, C Alpigiani, M Alstaty, B Alvarez Gonzalez, D Álvarez Piqueras, M G Alviggi, B T Amadio, K Amako, Y Amaral Coutinho, C Amelung, D Amidei, S P Amor Dos Santos, A Amorim, S Amoroso, G Amundsen, C Anastopoulos, L S Ancu, N Andari, T Andeen, C F Anders, G Anders, J K Anders, K J Anderson, A Andreazza, V Andrei, S Angelidakis, I Angelozzi, P Anger, A Angerami, F Anghinolfi, A V Anisenkov, N Anjos, A Annovi, C Antel, M Antonelli, A Antonov, F Anulli, M Aoki, L Aperio Bella, G Arabidze, Y Arai, J P Araque, A T H Arce, F A Arduh, J-F Arguin, S Argyropoulos, M Arik, A J Armbruster, L J Armitage, O Arnaez, H Arnold, M Arratia, O Arslan, A Artamonov, G Artoni, S Artz, S Asai, N Asbah, A Ashkenazi, B Åsman, L Asquith, K Assamagan, R Astalos, M Atkinson, N B Atlay, K Augsten, G Avolio, B Axen, M K Ayoub, G Azuelos, M A Baak, A E Baas, M J Baca, H Bachacou, K Bachas, M Backes, M Backhaus, P Bagiacchi, P Bagnaia, Y Bai, J T Baines, O K Baker, E M Baldin, P Balek, T Balestri, F Balli, W K Balunas, E Banas, Sw Banerjee, A A E Bannoura, L Barak, E L Barberio, D Barberis, M Barbero, T Barillari, M-S Barisits, T Barklow, N Barlow, S L Barnes, B M Barnett, R M Barnett, Z Barnovska-Blenessy, A Baroncelli, G Barone, A J Barr, L Barranco Navarro, F Barreiro, J Barreiro Guimarães da Costa, R Bartoldus, A E Barton, P Bartos, A Basalaev, A Bassalat, R L Bates, S J Batista, J R Batley, M Battaglia, M Bauce, F Bauer, H S Bawa, J B Beacham, M D Beattie, T Beau, P H Beauchemin, P Bechtle, H P Beck, K Becker, M Becker, M Beckingham, C Becot, A J Beddall, A Beddall, V A Bednyakov, M Bedognetti, C P Bee, L J Beemster, T A Beermann, M Begel, J K Behr, C Belanger-Champagne, A S Bell, G Bella, L Bellagamba, A Bellerive, M Bellomo, K Belotskiy, O Beltramello, N L Belyaev, O Benary, D Benchekroun, M Bender, K Bendtz, N Benekos, Y Benhammou, E Benhar Noccioli, J Benitez, D P Benjamin, J R Bensinger, S Bentvelsen, L Beresford, M Beretta, D Berge, E Bergeaas Kuutmann, N Berger, J Beringer, S Berlendis, N R Bernard, C Bernius, F U Bernlochner, T Berry, P Berta, C Bertella, G Bertoli, F Bertolucci, I A Bertram, C Bertsche, D Bertsche, G J Besjes, O Bessidskaia Bylund, M Bessner, N Besson, C Betancourt, S Bethke, A J Bevan, R M Bianchi, L Bianchini, M Bianco, O Biebel, D Biedermann, R Bielski, N V Biesuz, M Biglietti, J Bilbao De Mendizabal, T R V Billoud, H Bilokon, M Bindi, S Binet, A Bingul, C Bini, S Biondi, D M Bjergaard, C W Black, J E Black, K M Black, D Blackburn, R E Blair, J-B Blanchard, T Blazek, I Bloch, C Blocker, W Blum, U Blumenschein, S Blunier, G J Bobbink, V S Bobrovnikov, S S Bocchetta, A Bocci, C Bock, M Boehler, D Boerner, J A Bogaerts, D Bogavac, A G Bogdanchikov, C Bohm, V Boisvert, P Bokan, T Bold, A S Boldyrev, M Bomben, M Bona, M Boonekamp, A Borisov, G Borissov, J Bortfeldt, D Bortoletto, V Bortolotto, K Bos, D Boscherini, M Bosman, J D Bossio Sola, J Boudreau, J Bouffard, E V Bouhova-Thacker, D Boumediene, C Bourdarios, S K Boutle, A Boveia, J Boyd, I R Boyko, J Bracinik, A Brandt, G Brandt, O Brandt, U Bratzler, B Brau, J E Brau, H M Braun, W D Breaden Madden, K Brendlinger, A J Brennan, L Brenner, R Brenner, S Bressler, T M Bristow, D Britton, D Britzger, F M Brochu, I Brock, R Brock, G Brooijmans, T Brooks, W K Brooks, J Brosamer, E Brost, J H Broughton, P A Bruckman de Renstrom, D Bruncko, R Bruneliere, A Bruni, G Bruni, L S Bruni, B H Brunt, M Bruschi, N Bruscino, P Bryant, L Bryngemark, T Buanes, Q Buat, P Buchholz, A G Buckley, I A Budagov, F Buehrer, M K Bugge, O Bulekov, D Bullock, H Burckhart, S Burdin, C D Burgard, B Burghgrave, K Burka, S Burke, I Burmeister, J T P Burr, E Busato, D Büscher, V Büscher, P Bussey, J M Butler, C M Buttar, J M Butterworth, P Butti, W Buttinger, A Buzatu, A R Buzykaev, S Cabrera Urbán, D Caforio, V M Cairo, O Cakir, N Calace, P Calafiura, A Calandri, G Calderini, P Calfayan, G Callea, L P Caloba, S Calvente Lopez, D Calvet, S Calvet, T P Calvet, R Camacho Toro, S Camarda, P Camarri, D Cameron, R Caminal Armadans, C Camincher, S Campana, M Campanelli, A Camplani, A Campoverde, V Canale, A Canepa, M Cano Bret, J Cantero, R Cantrill, T Cao, M D M Capeans Garrido, I Caprini, M Caprini, M Capua, R Caputo, R M Carbone, R Cardarelli, F Cardillo, I Carli, T Carli, G Carlino, L Carminati, S Caron, E Carquin, G D Carrillo-Montoya, J R Carter, J Carvalho, D Casadei, M P Casado, M Casolino, D W Casper, E Castaneda-Miranda, R Castelijn, A Castelli, V Castillo Gimenez, N F Castro, A Catinaccio, J R Catmore, A Cattai, J Caudron, V Cavaliere, E Cavallaro, D Cavalli, M Cavalli-Sforza, V Cavasinni, F Ceradini, L Cerda Alberich, B C Cerio, A S Cerqueira, A Cerri, L Cerrito, F Cerutti, M Cerv, A Cervelli, S A Cetin, A Chafaq, D Chakraborty, S K Chan, Y L Chan, P Chang, J D Chapman, D G Charlton, A Chatterjee, C C Chau, C A Chavez Barajas, S Che, S Cheatham, A Chegwidden, S Chekanov, S V Chekulaev, G A Chelkov, M A Chelstowska, C Chen, H Chen, K Chen, S Chen, S Chen, X Chen, Y Chen, H C Cheng, H J Cheng, Y Cheng, A Cheplakov, E Cheremushkina, R Cherkaoui El Moursli, V Chernyatin, E Cheu, L Chevalier, V Chiarella, G Chiarelli, G Chiodini, A S Chisholm, A Chitan, M V Chizhov, K Choi, A R Chomont, S Chouridou, B K B Chow, V Christodoulou, D Chromek-Burckhart, J Chudoba, A J Chuinard, J J Chwastowski, L Chytka, G Ciapetti, A K Ciftci, D Cinca, V Cindro, I A Cioara, C Ciocca, A Ciocio, F Cirotto, Z H Citron, M Citterio, M Ciubancan, A Clark, B L Clark, M R Clark, P J Clark, R N Clarke, C Clement, Y Coadou, M Cobal, A Coccaro, J Cochran, L Colasurdo, B Cole, A P Colijn, J Collot, T Colombo, G Compostella, P Conde Muiño, E Coniavitis, S H Connell, I A Connelly, V Consorti, S Constantinescu, G Conti, F Conventi, M Cooke, B D Cooper, A M Cooper-Sarkar, K J R Cormier, T Cornelissen, M Corradi, F Corriveau, A Corso-Radu, A Cortes-Gonzalez, G Cortiana, G Costa, M J Costa, D Costanzo, G Cottin, G Cowan, B E Cox, K Cranmer, S J Crawley, G Cree, S Crépé-Renaudin, F Crescioli, W A Cribbs, M Crispin Ortuzar, M Cristinziani, V Croft, G Crosetti, A Cueto, T Cuhadar Donszelmann, J Cummings, M Curatolo, J Cúth, H Czirr, P Czodrowski, G D'amen, S D'Auria, M D'Onofrio, M J Da Cunha Sargedas De Sousa, C Da Via, W Dabrowski, T Dado, T Dai, O Dale, F Dallaire, C Dallapiccola, M Dam, J R Dandoy, N P Dang, A C Daniells, N S Dann, M Danninger, M Dano Hoffmann, V Dao, G Darbo, S Darmora, J Dassoulas, A Dattagupta, W Davey, C David, T Davidek, M Davies, P Davison, E Dawe, I Dawson, R K Daya-Ishmukhametova, K De, R de Asmundis, A De Benedetti, S De Castro, S De Cecco, N De Groot, P de Jong, H De la Torre, F De Lorenzi, A De Maria, D De Pedis, A De Salvo, U De Sanctis, A De Santo, J B De Vivie De Regie, W J Dearnaley, R Debbe, C Debenedetti, D V Dedovich, N Dehghanian, I Deigaard, M Del Gaudio, J Del Peso, T Del Prete, D Delgove, F Deliot, C M Delitzsch, M Deliyergiyev, A Dell'Acqua, L Dell'Asta, M Dell'Orso, M Della Pietra, D Della Volpe, M Delmastro, P A Delsart, D A DeMarco, S Demers, M Demichev, A Demilly, S P Denisov, D Denysiuk, D Derendarz, J E Derkaoui, F Derue, P Dervan, K Desch, C Deterre, K Dette, P O Deviveiros, A Dewhurst, S Dhaliwal, A Di Ciaccio, L Di Ciaccio, W K Di Clemente, C Di Donato, A Di Girolamo, B Di Girolamo, B Di Micco, R Di Nardo, A Di Simone, R Di Sipio, D Di Valentino, C Diaconu, M Diamond, F A Dias, M A Diaz, E B Diehl, J Dietrich, S Diglio, A Dimitrievska, J Dingfelder, P Dita, S Dita, F Dittus, F Djama, T Djobava, J I Djuvsland, M A B do Vale, D Dobos, M Dobre, C Doglioni, J Dolejsi, Z Dolezal, B A Dolgoshein, M Donadelli, S Donati, P Dondero, J Donini, J Dopke, A Doria, M T Dova, A T Doyle, E Drechsler, M Dris, Y Du, J Duarte-Campderros, E Duchovni, G Duckeck, O A Ducu, D Duda, A Dudarev, E M Duffield, L Duflot, M Dührssen, M Dumancic, M Dunford, H Duran Yildiz, M Düren, A Durglishvili, D Duschinger, B Dutta, M Dyndal, C Eckardt, K M Ecker, R C Edgar, N C Edwards, T Eifert, G Eigen, K Einsweiler, T Ekelof, M El Kacimi, V Ellajosyula, M Ellert, S Elles, F Ellinghaus, A A Elliot, N Ellis, J Elmsheuser, M Elsing, D Emeliyanov, Y Enari, O C Endner, J S Ennis, J Erdmann, A Ereditato, G Ernis, J Ernst, M Ernst, S Errede, E Ertel, M Escalier, H Esch, C Escobar, B Esposito, A I Etienvre, E Etzion, H Evans, A Ezhilov, F Fabbri, L Fabbri, G Facini, R M Fakhrutdinov, S Falciano, R J Falla, J Faltova, Y Fang, M Fanti, A Farbin, A Farilla, C Farina, E M Farina, T Farooque, S Farrell, S M Farrington, P Farthouat, F Fassi, P Fassnacht, D Fassouliotis, M Faucci Giannelli, A Favareto, W J Fawcett, L Fayard, O L Fedin, W Fedorko, S Feigl, L Feligioni, C Feng, E J Feng, H Feng, A B Fenyuk, L Feremenga, P Fernandez Martinez, S Fernandez Perez, J Ferrando, A Ferrari, P Ferrari, R Ferrari, D E Ferreira de Lima, A Ferrer, D Ferrere, C Ferretti, A Ferretto Parodi, F Fiedler, A Filipčič, M Filipuzzi, F Filthaut, M Fincke-Keeler, K D Finelli, M C N Fiolhais, L Fiorini, A Firan, A Fischer, C Fischer, J Fischer, W C Fisher, N Flaschel, I Fleck, P Fleischmann, G T Fletcher, R R M Fletcher, T Flick, A Floderus, L R Flores Castillo, M J Flowerdew, G T Forcolin, A Formica, A Forti, A G Foster, D Fournier, H Fox, S Fracchia, P Francavilla, M Franchini, D Francis, L Franconi, M Franklin, M Frate, M Fraternali, D Freeborn, S M Fressard-Batraneanu, F Friedrich, D Froidevaux, J A Frost, C Fukunaga, E Fullana Torregrosa, T Fusayasu, J Fuster, C Gabaldon, O Gabizon, A Gabrielli, A Gabrielli, G P Gach, S Gadatsch, S Gadomski, G Gagliardi, L G Gagnon, P Gagnon, C Galea, B Galhardo, E J Gallas, B J Gallop, P Gallus, G Galster, K K Gan, J Gao, Y Gao, Y S Gao, F M Garay Walls, C García, J E García Navarro, M Garcia-Sciveres, R W Gardner, N Garelli, V Garonne, A Gascon Bravo, K Gasnikova, C Gatti, A Gaudiello, G Gaudio, L Gauthier, I L Gavrilenko, C Gay, G Gaycken, E N Gazis, Z Gecse, C N P Gee, Ch Geich-Gimbel, M Geisen, M P Geisler, C Gemme, M H Genest, C Geng, S Gentile, C Gentsos, S George, D Gerbaudo, A Gershon, S Ghasemi, H Ghazlane, M Ghneimat, B Giacobbe, S Giagu, P Giannetti, B Gibbard, S M Gibson, M Gignac, M Gilchriese, T P S Gillam, D Gillberg, G Gilles, D M Gingrich, N Giokaris, M P Giordani, F M Giorgi, F M Giorgi, P F Giraud, P Giromini, D Giugni, F Giuli, C Giuliani, M Giulini, B K Gjelsten, S Gkaitatzis, I Gkialas, E L Gkougkousis, L K Gladilin, C Glasman, J Glatzer, P C F Glaysher, A Glazov, M Goblirsch-Kolb, J Godlewski, S Goldfarb, T Golling, D Golubkov, A Gomes, R Gonçalo, J Goncalves Pinto Firmino Da Costa, G Gonella, L Gonella, A Gongadze, S González de la Hoz, G Gonzalez Parra, S Gonzalez-Sevilla, L Goossens, P A Gorbounov, H A Gordon, I Gorelov, B Gorini, E Gorini, A Gorišek, E Gornicki, A T Goshaw, C Gössling, M I Gostkin, C R Goudet, D Goujdami, A G Goussiou, N Govender, E Gozani, L Graber, I Grabowska-Bold, P O J Gradin, P Grafström, J Gramling, E Gramstad, S Grancagnolo, V Gratchev, P M Gravila, H M Gray, E Graziani, Z D Greenwood, C Grefe, K Gregersen, I M Gregor, P Grenier, K Grevtsov, J Griffiths, A A Grillo, K Grimm, S Grinstein, Ph Gris, J-F Grivaz, S Groh, J P Grohs, E Gross, J Grosse-Knetter, G C Grossi, Z J Grout, L Guan, W Guan, J Guenther, F Guescini, D Guest, O Gueta, E Guido, T Guillemin, S Guindon, U Gul, C Gumpert, J Guo, Y Guo, R Gupta, S Gupta, G Gustavino, P Gutierrez, N G Gutierrez Ortiz, C Gutschow, C Guyot, C Gwenlan, C B Gwilliam, A Haas, C Haber, H K Hadavand, A Hadef, P Haefner, S Hageböck, Z Hajduk, H Hakobyan, M Haleem, J Haley, G Halladjian, G D Hallewell, K Hamacher, P Hamal, K Hamano, A Hamilton, G N Hamity, P G Hamnett, L Han, K Hanagaki, K Hanawa, M Hance, B Haney, P Hanke, R Hanna, J B Hansen, J D Hansen, M C Hansen, P H Hansen, K Hara, A S Hard, T Harenberg, F Hariri, S Harkusha, R D Harrington, P F Harrison, F Hartjes, N M Hartmann, M Hasegawa, Y Hasegawa, A Hasib, S Hassani, S Haug, R Hauser, L Hauswald, M Havranek, C M Hawkes, R J Hawkings, D Hayakawa, D Hayden, C P Hays, J M Hays, H S Hayward, S J Haywood, S J Head, T Heck, V Hedberg, L Heelan, S Heim, T Heim, B Heinemann, J J Heinrich, L Heinrich, C Heinz, J Hejbal, L Helary, S Hellman, C Helsens, J Henderson, R C W Henderson, Y Heng, S Henkelmann, A M Henriques Correia, S Henrot-Versille, G H Herbert, Y Hernández Jiménez, G Herten, R Hertenberger, L Hervas, G G Hesketh, N P Hessey, J W Hetherly, R Hickling, E Higón-Rodriguez, E Hill, J C Hill, K H Hiller, S J Hillier, I Hinchliffe, E Hines, R R Hinman, M Hirose, D Hirschbuehl, J Hobbs, N Hod, M C Hodgkinson, P Hodgson, A Hoecker, M R Hoeferkamp, F Hoenig, D Hohn, T R Holmes, M Homann, T M Hong, B H Hooberman, W H Hopkins, Y Horii, A J Horton, J-Y Hostachy, S Hou, A Hoummada, J Howarth, M Hrabovsky, I Hristova, J Hrivnac, T Hryn'ova, A Hrynevich, C Hsu, P J Hsu, S-C Hsu, D Hu, Q Hu, S Hu, Y Huang, Z Hubacek, F Hubaut, F Huegging, T B Huffman, E W Hughes, G Hughes, M Huhtinen, P Huo, N Huseynov, J Huston, J Huth, G Iacobucci, G Iakovidis, I Ibragimov, L Iconomidou-Fayard, E Ideal, P Iengo, O Igonkina, T Iizawa, Y Ikegami, M Ikeno, Y Ilchenko, D Iliadis, N Ilic, T Ince, G Introzzi, P Ioannou, M Iodice, K Iordanidou, V Ippolito, N Ishijima, M Ishino, M Ishitsuka, R Ishmukhametov, C Issever, S Istin, F Ito, J M Iturbe Ponce, R Iuppa, W Iwanski, H Iwasaki, J M Izen, V Izzo, S Jabbar, B Jackson, P Jackson, V Jain, K B Jakobi, K Jakobs, S Jakobsen, T Jakoubek, D O Jamin, D K Jana, E Jansen, R Jansky, J Janssen, M Janus, G Jarlskog, N Javadov, T Javůrek, M Javurkova, F Jeanneau, L Jeanty, G-Y Jeng, D Jennens, P Jenni, C Jeske, S Jézéquel, H Ji, J Jia, H Jiang, Y Jiang, S Jiggins, J Jimenez Pena, S Jin, A Jinaru, O Jinnouchi, P Johansson, K A Johns, W J Johnson, K Jon-And, G Jones, R W L Jones, S Jones, T J Jones, J Jongmanns, P M Jorge, J Jovicevic, X Ju, A Juste Rozas, M K Köhler, A Kaczmarska, M Kado, H Kagan, M Kagan, S J Kahn, T Kaji, E Kajomovitz, C W Kalderon, A Kaluza, S Kama, A Kamenshchikov, N Kanaya, S Kaneti, L Kanjir, V A Kantserov, J Kanzaki, B Kaplan, L S Kaplan, A Kapliy, D Kar, K Karakostas, A Karamaoun, N Karastathis, M J Kareem, E Karentzos, M Karnevskiy, S N Karpov, Z M Karpova, K Karthik, V Kartvelishvili, A N Karyukhin, K Kasahara, L Kashif, R D Kass, A Kastanas, Y Kataoka, C Kato, A Katre, J Katzy, K Kawade, K Kawagoe, T Kawamoto, G Kawamura, V F Kazanin, R Keeler, R Kehoe, J S Keller, J J Kempster, H Keoshkerian, O Kepka, B P Kerševan, S Kersten, R A Keyes, M Khader, F Khalil-Zada, A Khanov, A G Kharlamov, T J Khoo, V Khovanskiy, E Khramov, J Khubua, S Kido, C R Kilby, H Y Kim, S H Kim, Y K Kim, N Kimura, O M Kind, B T King, M King, S B King, J Kirk, A E Kiryunin, T Kishimoto, D Kisielewska, F Kiss, K Kiuchi, O Kivernyk, E Kladiva, M H Klein, M Klein, U Klein, K Kleinknecht, P Klimek, A Klimentov, R Klingenberg, J A Klinger, T Klioutchnikova, E-E Kluge, P Kluit, S Kluth, J Knapik, E Kneringer, E B F G Knoops, A Knue, A Kobayashi, D Kobayashi, T Kobayashi, M Kobel, M Kocian, P Kodys, T Koffas, E Koffeman, N M Köhler, T Koi, H Kolanoski, M Kolb, I Koletsou, A A Komar, Y Komori, T Kondo, N Kondrashova, K Köneke, A C König, T Kono, R Konoplich, N Konstantinidis, R Kopeliansky, S Koperny, L Köpke, A K Kopp, K Korcyl, K Kordas, A Korn, A A Korol, I Korolkov, E V Korolkova, O Kortner, S Kortner, T Kosek, V V Kostyukhin, A Kotwal, A Kourkoumeli-Charalampidi, C Kourkoumelis, V Kouskoura, A B Kowalewska, R Kowalewski, T Z Kowalski, C 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Vazquez Schroeder, J Veatch, V Veeraraghavan, L M Veloce, F Veloso, S Veneziano, A Ventura, M Venturi, N Venturi, A Venturini, V Vercesi, M Verducci, W Verkerke, J C Vermeulen, A Vest, M C Vetterli, O Viazlo, I Vichou, T Vickey, O E Vickey Boeriu, G H A Viehhauser, S Viel, L Vigani, M Villa, M Villaplana Perez, E Vilucchi, M G Vincter, V B Vinogradov, C Vittori, I Vivarelli, S Vlachos, M Vlasak, M Vogel, P Vokac, G Volpi, M Volpi, H von der Schmitt, E von Toerne, V Vorobel, K Vorobev, M Vos, R Voss, J H Vossebeld, N Vranjes, M Vranjes Milosavljevic, V Vrba, M Vreeswijk, R Vuillermet, I Vukotic, Z Vykydal, P Wagner, W Wagner, H Wahlberg, S Wahrmund, J Wakabayashi, J Walder, R Walker, W Walkowiak, V Wallangen, C Wang, C Wang, F Wang, H Wang, H Wang, J Wang, J Wang, K Wang, R Wang, S M Wang, T Wang, T Wang, W Wang, X Wang, C Wanotayaroj, A Warburton, C P Ward, D R Wardrope, A Washbrook, P M Watkins, A T Watson, M F Watson, G Watts, S Watts, B M Waugh, S Webb, M S Weber, S W Weber, J S Webster, A R Weidberg, B Weinert, J Weingarten, C Weiser, H Weits, P S Wells, T Wenaus, T Wengler, S Wenig, N Wermes, M Werner, M D Werner, P Werner, M Wessels, J Wetter, K Whalen, N L Whallon, A M Wharton, A White, M J White, R White, D Whiteson, F J Wickens, W Wiedenmann, M Wielers, P Wienemann, C Wiglesworth, L A M Wiik-Fuchs, A Wildauer, F Wilk, H G Wilkens, H H Williams, S Williams, C Willis, S Willocq, J A Wilson, I Wingerter-Seez, F Winklmeier, O J Winston, B T Winter, M Wittgen, J Wittkowski, T M H Wolf, M W Wolter, H Wolters, S D Worm, B K Wosiek, J Wotschack, M J Woudstra, K W Wozniak, M Wu, M Wu, S L Wu, X Wu, Y Wu, T R Wyatt, B M Wynne, S Xella, D Xu, L Xu, B Yabsley, S Yacoob, D Yamaguchi, Y Yamaguchi, A Yamamoto, S Yamamoto, T Yamanaka, K Yamauchi, Y Yamazaki, Z Yan, H Yang, H Yang, Y Yang, Z Yang, W-M Yao, Y C Yap, Y Yasu, E Yatsenko, K H Yau Wong, J Ye, S Ye, I Yeletskikh, A L Yen, E Yildirim, K Yorita, R Yoshida, K Yoshihara, C Young, C J S Young, S Youssef, D R Yu, J Yu, J M Yu, J Yu, L Yuan, S P Y Yuen, I Yusuff, B Zabinski, R Zaidan, A M Zaitsev, N Zakharchuk, J Zalieckas, A Zaman, S Zambito, L Zanello, D Zanzi, C Zeitnitz, M Zeman, A Zemla, J C Zeng, Q Zeng, K Zengel, O Zenin, T Ženiš, D Zerwas, D Zhang, F Zhang, G Zhang, H Zhang, J Zhang, L Zhang, R Zhang, R Zhang, X Zhang, Z Zhang, X Zhao, Y Zhao, Z Zhao, A Zhemchugov, J Zhong, B Zhou, C Zhou, L Zhou, L Zhou, M Zhou, N Zhou, C G Zhu, H Zhu, J Zhu, Y Zhu, X Zhuang, K Zhukov, A Zibell, D Zieminska, N I Zimine, C Zimmermann, S Zimmermann, Z Zinonos, M Zinser, M Ziolkowski, L Živković, G Zobernig, A Zoccoli, M Zur Nedden, L Zwalinski
A measurement of the calorimeter response to isolated charged hadrons in the ATLAS detector at the LHC is presented. This measurement is performed with 3.2 nb[Formula: see text] of proton-proton collision data at [Formula: see text] [Formula: see text] from 2010 and 0.1 nb[Formula: see text] of data at [Formula: see text] [Formula: see text] from 2012. A number of aspects of the calorimeter response to isolated hadrons are explored. After accounting for energy deposited by neutral particles, there is a 5% discrepancy in the modelling, using various sets of Geant4 hadronic physics models, of the calorimeter response to isolated charged hadrons in the central calorimeter region...
2017: European Physical Journal. C, Particles and Fields
https://www.readbyqxmd.com/read/28258523/analysis-of-pelagia-noctiluca-proteome-reveals-a-red-fluorescent-protein-a-zinc-metalloproteinase-and-a-peroxiredoxin
#7
Bárbara Frazão, Alexandre Campos, Hugo Osório, Benjamin Thomas, Sérgio Leandro, Alexandre Teixeira, Vitor Vasconcelos, Agostinho Antunes
Pelagia noctiluca is the most venomous jellyfish in the Mediterranean Sea where it forms dense blooms. Although there is several published research on this species, until now none of the works has been focused on a complete protein profile of the all body constituents of this organism. Here, we have performed a detailed proteomics characterization of the major protein components expressed by P. noctiluca. With that aim, we have considered the study of jellyfish proteins involved in defense, body constituents and metabolism, and furthered explore the significance and potential application of such bioactive molecules...
March 3, 2017: Protein Journal
https://www.readbyqxmd.com/read/28256723/immunoblotting-and-immunodetection
#8
Duojiao Ni, Peng Xu, Sean Gallagher
Immunoblotting (western blotting) is used to identify specific antigens recognized by polyclonal or monoclonal antibodies. This unit provides protocols for all steps, starting with solubilization of the protein samples, usually by means of SDS and reducing agents. Following solubilization, the material is separated by SDS-PAGE and the antigens are electrophoretically transferred to a membrane, a process that can be monitored by reversible staining with Ponceau S. The transferred proteins are bound to the surface of the membrane, providing access to immunodetection reagents...
March 3, 2017: Current Protocols in Cell Biology
https://www.readbyqxmd.com/read/28255019/a-risk-factor-analysis-of-outcomes-after-unrelated-cord-blood-transplantation-for-children-with-wiskott-aldrich-syndrome
#9
Zhanna Shekhovtsova, Carmem Bonfim, Annalisa Ruggeri, Samantha Nichele, Kristin Page, Amal AlSeraihy, Francisco Barriga, José Sánchez de Toledo Codina, Paul Veys, Jaap Jan Boelens, Karin Mellgren, Henrique Bittencourt, Tracey O' Brien, Peter J Shaw, Alicja Chybicka, Fernanda Volt, Federica Giannotti, Eliane Gluckman, Joanne Kurtzberg, Andrew R Gennery, Vanderson Rocha
Wiskott-Aldrich syndrome is a severe X-linked recessive immune deficiency disorder. A scoring system of Wiskott-Aldrich syndrome severity (0.5-5) distinguishes 2 phenotypes: X-linked thrombocytopenia and classic Wiskott-Aldrich syndrome. Hematopoietic cell transplantation is curative for Wiskott-Aldrich syndrome, however the use of unrelated umbilical cord blood transplantation has seldom been described. We analyzed umbilical cord blood transplantation outcomes for 90 patients. Median age at umbilical cord blood transplantation was 1...
March 2, 2017: Haematologica
https://www.readbyqxmd.com/read/28254415/functional-reconstitution-of-human-equilibrative-nucleoside-transporter-1-into-styrene-maleic-acid-co-polymer-lipid-particles
#10
Shahid Rehan, Ville O Paavilainen, Veli-Pekka Jaakola
The human equilibrative nucleoside transporter-1 (hENT1) is important for the entry of anti-cancer and anti-viral nucleoside analog therapeutics into the cell, and thus for their efficacy. Understanding of hENT1 structure-function relationship could assist with development of nucleoside analogs with better cellular uptake properties. However, structural and biophysical studies of hENT1 remain challenging as the hydrophobic nature of the protein leads to complete aggregation upon detergent-based membrane isolation...
February 28, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28235606/anti-tumor-effect-of-polysaccharide-from-hirsutella-sinensis-on-human-non-small-cell-lung-cancer-and-nude-mice-through-intrinsic-mitochondrial-pathway
#11
Wen-Bin Liu, Fei Xie, Hui-Qing Sun, Meng Meng, Zhen-Yuan Zhu
Our previous works had proved the structural properties of Hirsutella sinensis polysaccharide-III(HSP-III). Herein, its anti-tumor effect on lung cancer correlated with mitochondrial apoptosis pathway was investigated. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay showed that HSP-III induces the apoptosis of H1299 cells; however the proliferation viability of normal lung epithelial cells is not affected. HSP-III treatment collapses the H1299 cell mitochondrial membrane potential, and western blot analysis of cytochrome C, Bax, caspase-3 and caspase-9 further indicates that apoptotic effects induced by HSP-III is through the mitochondrial pathway...
February 22, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28182792/somatostatin-receptor-staining-in-ffpe-sections-using-a-ligand-derivative-dye-as-an-alternative-to-immunostaining
#12
Koki Hasegawa, Shinji Kudoh, Takaaki Ito
The confirmation of target expression in tissues is a prerequisite for molecular-targeted therapy. However, difficulties are sometimes associated with the production of appropriate antibodies against receptors. We herein developed a ligand derivative dye for the staining of receptors. The somatostatin receptor (sstr) was selected as the target and FITC-octreotate as the detective agent. We performed a blot analysis to detect sstr in the transfer membrane. The sstr2 recombinant protein or cell lysate from a small cell lung carcinoma cell line (H69) was boiled and loaded onto SDS-PAGE, and the proteins were transferred to a membrane...
2017: PloS One
https://www.readbyqxmd.com/read/28173785/hcv-screening-in-a-cohort-of-hiv-infected-and-uninfected-homeless-and-marginally-housed-women-in-san-francisco-california
#13
Kimberly Page, Michelle Yu, Jennifer Cohen, Jennifer Evans, Martha Shumway, Elise D Riley
BACKGROUND: Hepatitis C virus (HCV) screening has taken on new importance as a result of updated guidelines and new curative therapies. Relatively few studies have assessed HCV infection in homeless populations, and a minority include women. We assessed prevalence and correlates of HCV exposure in a cohort of homeless and unstably housed women in San Francisco, and estimated the proportion undiagnosed. METHODS: A probability sample of 246 women were recruited at free meal programs, homeless shelters, and low-cost single room occupancy hotels in San Francisco; women with HIV were oversampled...
February 7, 2017: BMC Public Health
https://www.readbyqxmd.com/read/28164808/emerging-food-allergens-identification-of-polyphenol-oxidase-as-an-important-allergen-in-eggplant-solanum-melongena-l
#14
Bheemanapalli N Harish Babu, Anthony Wilfred, Yeldur P Venkatesh
Although many allergens have been detected in eggplant (Solanum melongena L.), their identity have not been elucidated. The aim of this study was to investigate whether polyphenol oxidase (PPO), an important eggplant enzyme, acts as an allergen. The proteins of eggplant peel extract were separated on phenyl-Sepharose (PS), and analyzed by skin prick test (SPT), ELISA and IgE-immunoblotting; the components were analyzed for PPO activity, presence of protein-bound copper, and recognition by rabbit polyclonal anti-sweet potato PPO antiserum...
February 2017: Immunobiology
https://www.readbyqxmd.com/read/28164534/expression-of-hcv-alternative-reading-frame-protein-core-1-f-in-baculovirus-expression-system-and-its-evaluation-for-assessment-of-specific-anti-core-1-antibody-in-iranian-hcv-infected-patients
#15
Pooneh Rahimi, Rouhollah Vahabpour, Farideh Sadat Sajadian Fard, Fatemeh Motevalli, Fatemeh Fotouhi Chahouki, Mohammad Reza Aghasadeghi, Azam Bolhassani, Seyed Mehdi Sadat, Ehsan Mostafavi, Nasir Mohajel, Ali Jahanian-Najafabadi, Mohammad Reza Amiran
BACKGROUND: Hepatitis C virus (HCV) genome contains an overlapping reading frame which results in alternative core protein (ARFP). Baculovirus expression system was used as a powerful eukaryotic vector system to express core+1/F protein for the first time. This recombinant core+1/F protein was used to assess the anti-core+1 antibody in anti-HCV drug resistant and sustained virologic response (SVR) patients. METHODS: The core+1 coding sequence from HCV genotype 1 was designed and synthesized in pUC57 vector...
October 1, 2016: Clinical Laboratory
https://www.readbyqxmd.com/read/28163123/isolation-and-characterization-of-a-novel-metalloprotease-inhibitor-from-bothrops-alternatus-snake-serum
#16
Tatiana Z Palacio, Norival A Santos-Filho, José Cesar Rosa, Rui S Ferreira, Benedito Barraviera, Suely V Sampaio
Resistance of snakes and some other animals to snake envenomation has been attributed to soluble factors present in their tissues. Here we report the isolation of a novel metalloprotease inhibitor from Bothrops alternatus snake serum (named BaltMPI) with high purity, using a four-step chromatographic method. BaltMPI has molecular weights of 60.5 and 42.4kDa, as determined by SDS-PAGE and mass spectrometry, respectively, and pI=5.27. The first 60 amino acids from the N-terminal region of BaltMPI, determined by Edman's degradation, showed high homology (97%) with the snake venom metalloprotease inhibitor (SVMPI) BJ46a and other SVMPIs (78-82%)...
February 3, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28129116/a-proof-of-concept-for-epigenetic-therapy-of-tissue-fibrosis-inhibition-of-liver-fibrosis-progression-by-3-deazaneplanocin-a
#17
Müjdat Zeybel, Saimir Luli, Laura Sabater, Timothy Hardy, Fiona Oakley, Jack Leslie, Agata Page, Eva Moran Salvador, Victoria Sharkey, Hidekazu Tsukamoto, David C K Chu, Uma Sharan Singh, Mirco Ponzoni, Patrizia Perri, Daniela Di Paolo, Edgar J Mendivil, Jelena Mann, Derek A Mann
The progression of fibrosis in chronic liver disease is dependent upon hepatic stellate cells (HSCs) transdifferentiating to a myofibroblast-like phenotype. This pivotal process is controlled by enzymes that regulate histone methylation and chromatin structure, which may be targets for developing anti-fibrotics. There is limited pre-clinical experimental support for the potential to therapeutically manipulate epigenetic regulators in fibrosis. In order to learn if epigenetic treatment can halt the progression of pre-established liver fibrosis, we treated mice with the histone methyltransferase inhibitor 3-deazaneplanocin A (DZNep) in a naked form or by selectively targeting HSC-derived myofibroblasts via an antibody-liposome-DZNep targeting vehicle...
January 4, 2017: Molecular Therapy: the Journal of the American Society of Gene Therapy
https://www.readbyqxmd.com/read/28101473/purification-and-characterization-of-bovine-serum-albumin-using-chromatographic-method
#18
Sanaz Balkani, Sara Shamekhi, Ramin Raoufinia, Reza Parvan, Jalal Abdolalizadeh
Purpose: Albumin is an abundant protein of blood and has many biopharmaceutical applications. The aim of this study was to purify bovine serum albumin (BSA) using produced rabbit anti-BSA antibody. Methods: The polyclonal antibody was produced against the BSA in rabbits. Then, the pure BSA was injected to three white New Zealand rabbits. ELISA test was done to evaluate antibody production. After antibody purification,the purified antibody was attached to CNBr-activated sepharose and finally it was used for purification of albumin from bovine serum...
December 2016: Advanced Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/28101463/development-of-a-novel-human-single-chain-antibody-against-egfrviii-antigen-by-phage-display-technology
#19
Leila Rahbarnia, Safar Farajnia, Hossein Babaei, Jafar Majidi, Bahman Akbari, Shiva Ahdi Khosroshahi
Purpose: EGFRvIII as the most common mutant variant of the epidermal growth factor receptor is resulting from deletion of exons 2-7 in the coding sequence and junction of exons 1 and 8 through a novel glycine residue. EGFRvIII is highly expressed in glioblastoma, carcinoma of the breast, ovary, and lung but not in normal cells. The aim of the present study was identification of a novel single chain antibody against EGFRvIII as a promising target for cancer therapy. Methods: In this study, a synthetic peptide corresponding to EGFRvIII protein was used for screening a naive human scFv phage library...
December 2016: Advanced Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/28090276/overexpression-of-recombinant-human-teriparatide-rhpth-1-34-in-escherichia-coli-an-innovative-gene-fusion-approach
#20
Nahid Bakhtiari, Zahra Amini Bayat, Sepideh Sagharidouz, Mohsen Vaez
BACKGROUND: Parathyroid hormone is an 84-amino acid peptide secreted by the parathyroid glands. Its physiological role is maintenance of normal serum calcium level and bone remodeling. Biological activity of this hormone is related to N-terminal 1-34 amino acids. The recombinant form of hormone (1-34) has been approved for treatment of osteoporosis from 2002. In this study, a novel fusion partner has been developed for preparation of high yield recombinant 1-34 amino acids of hPTH. METHODS: Novel nucleotide cassette designed encoding a chimeric fusion protein comprising of a fusion partner consisting of a His-tag in N-terminal, 53 amino acids belong to Escherichia coli (E...
January 2017: Avicenna Journal of Medical Biotechnology
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