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https://www.readbyqxmd.com/read/28240008/role-of-plasticity-region-genes-and-cage-gene-of-cagpai-of-helicobacter-pylori-in-development-of-gastrointestinal-gi-diseases
#1
Shokufe GholizadeTobnagh, Seyedeh Zahra Bakhti, Saeid Latifi Navid, Saber Zahri, Fatemeh Sadat Bakhti
Background: Helicobacter pylori is a Gram-negative, micro aerophilic bacterium in the human stomach that is associated with the development of gastrointestinal ailments such as peptic ulcer (PU) and gastric cancer (GC). In the present study, plasticity region genes (jhp0940, jhp0945 and jhp0947) and and cagE gene of cagPAI were assessed independently and in combination for their ability to predict clinical consequences. Materials and Methods: A total of 211 strains which were isolated from patients with different gastrointestinal diseases (114 with non-atrophic gastritis, 59 with PU, and 38 with GC) were genotyped by PCR and sequencing...
1, 2017: Asian Pacific Journal of Cancer Prevention: APJCP
https://www.readbyqxmd.com/read/28240002/cholesterol-homeostasis-in-isolated-lymphocytes-a-differential-correlation-between-male-control-and-chronic-lymphocytic-leukemia-subjects
#2
Shrimanjunath Sankanagoudar, Govind Singh, Manaranjan Mahapatra, Lalit Kumar, Nimai Chand Chandra
Background: This study was performed to investigate any association between cellular cholesterol homeostasis and chronic lymphocytic leukemia (CLL). CLL is characterized primarily by an abnormal accumulation of neoplastic B cells in the blood, bone marrow, lymph nodes and spleen. Methods: Men aged >50 years participated in this study. Enzyme-based plasma lipid profile estimations, peripheral blood lymphocyte isolation, lysate preparations, SDS-PAGE, western blotting, dil-LDL uptake and ultracentrifugation were employed...
1, 2017: Asian Pacific Journal of Cancer Prevention: APJCP
https://www.readbyqxmd.com/read/28239988/novel-serotype-of-bluetongue-virus-in-south-america-and-first-report-of-epizootic-haemorrhagic-disease-virus-in-ecuador
#3
J Verdezoto, E Breard, C Viarouge, H Quenault, P Lucas, C Sailleau, S Zientara, D Augot, S Zapata
Bluetongue virus (BTV) and Epizootic haemorrhagic disease virus (EHDV) are closely related Orbiviruses that affect domestic and wild ruminants. In Ecuador previous serological studies reported the presence of BTV; however, no data are available about the presence of EHDV. In this study, 295 cattle without symptoms of infection were sampled from two farms located in Andean and Amazonian regions and from a slaughterhouse in the coastal region. ELISA analyses showed high prevalence of BTV (98.9%) and EHDV (81...
February 26, 2017: Transboundary and Emerging Diseases
https://www.readbyqxmd.com/read/28239979/beyond-sodium-phosphate-and-potassium-potential-dietary-interventions-in-kidney-disease
#4
EDITORIAL
Jaimon T Kelly, Megan Rossi, David W Johnson, Katrina L Campbell
People with kidney disease are advised to restrict individual nutrients, such as sodium, potassium, and phosphate, in line with current best practice guidelines. However, there is limited evidence to support the efficacy of single nutrient strategies, and compliance remains a challenge for clinicians to overcome. Many factors contribute to poor compliance with dietary prescriptions, including conflicting priorities for single nutrient restriction, the arduous self-monitoring required, and the health-related knock-on effects resulting from targeting these nutrients in isolation...
February 26, 2017: Seminars in Dialysis
https://www.readbyqxmd.com/read/28239936/the-cancer-care-experiences-of-gay-lesbian-and-bisexual-patients-a-secondary-analysis-of-data-from-the-uk-cancer-patient-experience-survey
#5
N J Hulbert-Williams, C O Plumpton, P Flowers, R McHugh, R D Neal, J Semlyen, L Storey
Understanding the effects of population diversity on cancer-related experiences is a priority in oncology care. Previous research demonstrates inequalities arising from variation in age, gender and ethnicity. Inequalities and sexual orientation remain underexplored. Here, we report, for the first time in the UK, a quantitative secondary analysis of the 2013 UK National Cancer Patient Experience Survey which contains 70 questions on specific aspects of care, and six on overall care experiences. 68,737 individuals responded, of whom 0...
February 27, 2017: European Journal of Cancer Care
https://www.readbyqxmd.com/read/28239935/isolation-and-characterization-of-an-atypical-siberian-sturgeon-herpesvirus-strain-in-russia-novel-north-american-acipenserid-herpesvirus-2-strain-in-europe
#6
A Doszpoly, I M Kalabekov, R Breyta, I S Shchelkunov
Siberian sturgeon herpesvirus (SbSHV) was isolated in Russia for the first time in 2006. Nine SbSHV isolates were recovered from different fish hatcheries producing the same cytopathic effect in cell cultures, the same clinical signs and mortality kinetics in virus-infected fish and the same virus neutralization pattern and shared identical nucleotide sequences. In 2011, a new isolate was recovered from juvenile sturgeon, which caused completely different cytopathic effect. That isolate was not readily neutralized by Siberian sturgeon hyperimmune antisera, and its DNA was not recognized by the routine PCR developed for SbSHV detection...
February 27, 2017: Journal of Fish Diseases
https://www.readbyqxmd.com/read/28239908/pathogenicity-of-conidiobolus-coronatus-and-fusarium-solani-in-mouse-models
#7
Yadi Li, Xiangang Fang, Xiaoqian Zhou, Suying Geng, Yuxin Wang, Xiumin Yang
To study the pathogenicity of Conidiobolus coronatus (C. coronatus) and Fusarium solani (F. solani) in animal models. Immunocompromised mice were treated with cyclophosphamide and prednisolone via intraperitoneal injection before and after inoculation. According to pathogenic characteristics of different fungi, C. coronatus was used to infect mice via intravenous inoculation, intraperitoneal inoculation, gastrointestinal infusion and intradermal inoculation methods. And F. solani was used to infect mice by inoculation via the abraded or normal skin...
February 27, 2017: Mycoses
https://www.readbyqxmd.com/read/28239874/habitat-segregation-and-cryptic-adaptation-of-species-of-periophthalmus-gobioidei-gobiidae
#8
G Polgar, S Zaccara, M Babbucci, F Fonzi, C M Antognazza, N Ishak, Z Sulaiman, G Crosa
A study was conducted on the habitat distribution of four sympatric species of Periophthalmus (the silver-lined mudskipper Periophthalmus argentilineatus, the slender mudskipper Periophthalmus gracilis, the kalolo mudskipper Periophthalmus kalolo and the Malacca mudskipper Periophthalmus malaccensis) from northern Sulawesi. Molecular phylogenetic reconstructions based on one mtDNA marker (16S) were used to validate the morphological taxa, identifying five molecular clades. Periophthalmus argentilineatus includes two molecular species, which are named Periophthalmus argentilineatus clades F and K...
February 27, 2017: Journal of Fish Biology
https://www.readbyqxmd.com/read/28239807/adiponectin-partially-rescues-high-glucose-high-fat-induced-impairment-of-mitochondrial-biogenesis-and-function-in-a-pgc-1%C3%AE-dependent-manner
#9
H Wang, W-J Yan, J-L Zhang, F-Y Zhang, C Gao, Y-J Wang, W Bond Law, L Tao
OBJECTIVE: Plasma adiponectin (APN) levels are decreased in diabetic patients. Dysfunctional mitochondrial biogenesis is involved in type 2 diabetes (T2DM) pathogenesis, by unclear mechanisms. The present study determined (1) whether myocardial mitochondrial biogenesis was impaired in cardiomyocytes exposed to a high glucose/high fat (HGHF) medium (a T2DM in vitro model), (2) the effects of APN administration upon mitochondrial biogenesis in cardiomyocytes affected by HGHF incubation, and 3) the involved underlying mechanisms...
January 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28239798/effect-of-fibroblast-like-cells-of-mesenchymal-origin-of-cytotoxic-activity-of-lymphocytes-against-nk-sensitive-target-cells
#10
A Yu Lupatov, Ya S Kim, O A Bystrykh, I V Vakhrushev, S V Pavlovich, K N Yarygin, G T Sukhikh
We studied immunosuppressive properties of skin fibroblasts and mesenchymal stromal cells against NK cells. In vitro experiments showed that mesenchymal stromal cells isolated from human umbilical cord and human skin fibroblasts can considerably attenuate cytotoxic activity of NK cells against Jurkat cells sensitive to NK-mediated lysis. NK cells cultured in lymphocyte population exhibited higher cytotoxic activity than isolated NK cells. Mesenchymal stromal cells or fibroblasts added 1:1 to lymphocyte culture almost completely suppressed NK cell cytotoxicity...
February 27, 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28239797/characteristics-of-multipotent-mesenchymal-stromal-cells-isolated-from-the-endometrium-and-endometriosis-lesions-of-women-with-malformations-of-the-internal-reproductive-organs
#11
A M Savilova, K N Farkhat, M N Yushina, Yu V Rudimova, Z N Makiyan, L V Adamyan
We isolated and characterized cell cultures from eutopic endometrium and endometriotic lesions of women with malformations of the internal reproductive organs. The cells had fibroblast-like shape and intensively expressed CD90, CD73, CD105, CD44, CD146, and CD117 and were capable of induced adipogenic and osteogenic differentiation in vitro. The obtained cultures exhibited properties of multipotent mesenchymal stromal cells; at the same time, they demonstrated in vitro immunophenotypic differences from cell cultures of eutopic and ectopic endometrium of women without developmental abnormalities, which suggests their functional difference...
February 27, 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28239758/acellular-mouse-kidney-ecm-can-be-used-as-a-three-dimensional-substrate-to-test-the-differentiation-potential-of-embryonic-stem-cell-derived-renal-progenitors
#12
Manpreet Sambi, Theresa Chow, Jennifer Whiteley, Mira Li, Shawn Chua, Vanessa Raileanu, Ian M Rogers
The development of strategies for tissue regeneration and bio-artificial organ development is based on our understanding of embryogenesis. Differentiation protocols attempt to recapitulate the signaling modalities of gastrulation and organogenesis, coupled with cell selection regimens to isolate the cells of choice. This strategy is impeded by the lack of optimal in vitro culture systems since traditional culture systems do not allow for the three-dimensional interaction between cells and the extracellular matrix...
February 27, 2017: Stem Cell Reviews
https://www.readbyqxmd.com/read/28239741/-impingement-syndromes-of-the-shoulder
#13
M Beirer, A B Imhoff, S Braun
In addition to tears of the rotator cuff, isolated impingement syndrome of the shoulder is the most common diagnosis in shoulder disorders. This is of high relevance in orthopedic sports medicine. In fact, impingement of the shoulder is not the diagnosis but rather a symptom of a functional or even a structural pathology. Detailed knowledge about the different types of impingement and the underlying causes is essential to provide adequate treatment. Primarily, impingement of the shoulder should be treated nonoperatively...
February 26, 2017: Der Orthopäde
https://www.readbyqxmd.com/read/28239733/evidence-of-robust-participation-by-an-equatorial-4-o-group-in-glycosylation-on-a-2-azido-2-deoxy-glucopyranosyl-donor
#14
Dongming Yao, Yichu Liu, Shiqiang Yan, Yingxia Li, Chun Hu, Ning Ding
Although there are numerous claims of remote group participation leading to the synthesis of the expected glycosides with improved anomeric geometry outcome in glycosylation, there is still a lack of enough strong evidence and this has led to a long-term debate, particularly for equatorial 4-O group participation. In this work, we were able to synthesize and isolate a stable seven-membered trichlorooxazepine ring bridging intermediate with a high yield by employing a 2-azido-2-deoxy-glucopyranosyl donor, which provides strong evidence to support the putative participation of the equatorial 4-O group in glycosylation...
February 27, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28239717/simulations-and-spectra-of-water-in-co-matrices
#15
Rafael Escribano, Emilio Artacho, Akira Kouchi, Tetusya Hama, Yuki Kimura, Hiroshi Hidaka, Naoki Watanabe
Models for the inclusion of water molecules in carbon monoxide matrices are developed using density functional theory applied to amorphous solid systems. The models cover a large range of systems for smaller or larger CO matrices with different water content, consisting of either individual H2O molecules or small clusters linked by H-bonds. The vibrational spectra of the samples are predicted at the minimum of their potential energy surface. The spectra allow instances where the water molecules remain isolated or form aggregates to be discerned, and they also provide an indication of the strength of the H-bonding, when present...
February 27, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28239676/intact-ventral-striatal-prediction-error-signaling-in-medicated-schizophrenia-patients
#16
Adam J Culbreth, Andrew Westbrook, Ziye Xu, Deanna M Barch, James A Waltz
BACKGROUND: Midbrain dopaminergic neurons code a computational quantity, reward prediction error (RPE), which has been causally related to learning. Recently, this insight has been leveraged to link phenomenological and biological levels of understanding in psychiatric disorders, such as schizophrenia. However, results have been mixed, possibly due to small sample sizes. Here we present results from two studies with relatively large Ns to assess VS RPE in schizophrenia. METHODS: In the current study we analyzed data from two independent studies, involving a total of 87 chronic medicated schizophrenia patients and 61 controls...
September 2016: Biological Psychiatry: Cognitive Neuroscience and Neuroimaging
https://www.readbyqxmd.com/read/28239669/the-heterocellular-emergence-of-colorectal-cancer
#17
REVIEW
Christopher J Tape
Tissues contain multiple different cell types and can be considered to be heterocellular systems. Signaling between different cells allows tissues to achieve phenotypes that no cell type can achieve in isolation. Such emergent tissue-level phenotypes can be said to 'supervene upon' heterocellular signaling. It is proposed here that cancer is also an emergent phenotype that supervenes upon heterocellular signaling. Using colorectal cancer (CRC) as an example, I review how heterotypic cells differentially communicate to support emergent malignancy...
February 2017: Trends in Cancer
https://www.readbyqxmd.com/read/28239659/hsp90-regulation-of-fibroblast-activation-in-pulmonary-fibrosis
#18
Vishwaraj Sontake, Yunguan Wang, Rajesh K Kasam, Debora Sinner, Geereddy B Reddy, Anjaparavanda P Naren, Francis X McCormack, Eric S White, Anil G Jegga, Satish K Madala
Idiopathic pulmonary fibrosis (IPF) is a severe fibrotic lung disease associated with fibroblast activation that includes excessive proliferation, tissue invasiveness, myofibroblast transformation, and extracellular matrix (ECM) production. To identify inhibitors that can attenuate fibroblast activation, we queried IPF gene signatures against a library of small-molecule-induced gene-expression profiles and identified Hsp90 inhibitors as potential therapeutic agents that can suppress fibroblast activation in IPF...
February 23, 2017: JCI Insight
https://www.readbyqxmd.com/read/28239623/isolation-of-latex-bead-phagosomes-from-dictyostelium-for-in-vitro-functional-assays
#19
Ashwin D'Souza, Paulomi Sanghavi, Ashim Rai, Divya Pathak, Roop Mallik
We describe a protocol to purify latex bead phagosomes (LBPs) from Dictyostelium cells. These can be later used for various in vitro functional assays. For instance, we use these LBPs to understand the microtubule motor-driven transport on in vitro polymerized microtubules. Phagosomes are allowed to mature for defined periods inside cells before extraction for in vitro motility. These assays allow us to probe how lipids on the phagosome membrane recruit and organize motors, and also measure the motion and force generation resulting from underlying lipid-motor interactions...
December 5, 2016: Bio-protocol
https://www.readbyqxmd.com/read/28239566/biological-and-historical-overview-of-zika-virus
#20
REVIEW
Najealicka Armstrong, Wangheng Hou, Qiyi Tang
The recent outbreak of the Zika virus attracts worldwide attention probably because the most recently affected country (Brazil) will host the 2016 Olympic Game. Zika virus infected cases are now spreading to many other countries and its infection might be linked to some severe medical sequelae. Since its first isolation from the infected monkey in 1947 in Uganda, only a few studies had been taken until recent outbreak. According to the history of referenced publications, there is a 19-year gap from 1989 to 2007...
February 12, 2017: World Journal of Virology
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