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https://www.readbyqxmd.com/read/28109209/nostoc-thermotolerans-sp-nov-a-soil-dwelling-species-of-nostoc-cyanobacteria-isolated-from-madhya-pradesh-india
#1
Archana Suradkar, Chelsea Villanueva, Lira A Gaysina, Dale A Casamatta, Aniket Saraf, Gandhali Dighe, Ratnaprabha Mergu, Prashant Singh
A filamentous, soil dwelling cyanobacterial strain (9C-PS) was isolated from Mandsaur, Madhya Pradesh, India and is being described as new species of Nostoc. Extensive morphological and molecular characterization along with thorough assessment of ecology was performed. The style of filament orientation, type and nature of the sheath (e.g., distribution and visibility across the trichome), and vegetative and heterocyte cell dimensions and shape were assessed for more than a year employing both culture and naturally occurring samples...
January 19, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28109208/psychromonas-aquatilis-sp-nov-isolated-from-water-samples-obtained-in-the-chilean-antarctica
#2
Peter Kämpfer, Rute Irgang, Mathías Poblete-Morales, Stefanie P Glaeser, Marcelo Cortez-San Martín, Ruben Avendaño-Herrera
One slightly beige-white pigmented, Gram-staining negative, rod-shaped bacterium, strain M1A1T, was isolated from seawater samples obtained in Fildes Bay, Antarctica (62º12´S, 58º57´W). Phylogenetic analysis based on nearly full-length 16S rRNA gene sequences showed that the isolate shared 98.4 % 16S rRNA gene sequence identity to the type strain of the species Psychromonas arctica, but less than 97 % 16S rRNA gene sequence similarities to all other Psychromonas species. DNA-DNA hybridisation (DDH) with Psychromonas arctica DSM 14288T showed low values (21 %, reciprocal 27 %)...
January 19, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28109207/rhodohalobacter-halophilus-gen-nov-sp-nov-a-moderately-halophilic-member-of-the-family-balneolaceae
#3
Jun Xia, Zhi-Hong Xie, Christopher A Dunlap, Alejandro P Rooney, Zong-Jun Du
A novel Gram-stain-negative, rod-shaped, facultatively anaerobic, oxidase-negative and catalase-positive bacterium, designated JZ3C29T, was isolated from a saltern located in Feicheng, China. Strain JZ3C29T was tolerant to moderate salt conditions. Optimal growth occurred at 40 °C (range 20-50 °C) and pH 7.5-8.5 (range pH 7.0-9.0) with 8-10 % (w/v) NaCl (range 2-16 %). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain JZ3C29T shared highest similarity with Gracilimonas tropica CL-CB462T (90...
January 19, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28109206/proposal-of-mucilaginibacter-galii-sp-nov-isolated-from-leaves-of-galium-album
#4
Ebru L Aydogan, Hans-Jürgen Busse, Gerald Moser, Christoph Müller, Peter Kämpfer, Stefanie P Glaeser
A pale pink pigmented, Gram-stain-negative, rod-shaped, non-spore forming bacterial strain, PP-F2F-G47T, was isolated from the phyllosphere of the herbaceous plant Galium album. Phylogenetic analysis based on nearly full-length 16S rRNA gene sequence showed highest sequence similarity to type strains of the species Mucilaginibacter daejeonensis (96.2 %), Mucilaginibacter dorajii (95.7 %), and Mucilaginibacter phyllosphaerae (95.5 %). 16S rRNA gene-sequence similarities to all other type strains were below 95...
January 19, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28109205/haloterrigena-mahii-sp-nov-an-extreme-halophilic-archaeon-from-the-solar-saltern
#5
Jiun-Yan Ding, Sheng-Chung Chen, Mei-Chin Lai, Tsai-Ling Liao
A pleomorphic, gas vesicle containing, halophilic archaeon, designated strain H13T, was isolated from the solar saltern of the Western Salt Co., Chula Vista, California, USA. Cells of strain H13T were non-motile, rod-shaped and 3-10 μm in length. The optimum growth conditions were 3.5-5.0 M NaCl, 45-55 oC, and pH range of 6.5-8.2. The major polar lipids were C20C20 and C20C25 derivatives of phosphatidylglycerol (PG), phosphatidylglycerol phosphate methyl ester (PGP-Me) and disulfated diglycosyl diether-1 (S2-DGD-1)...
January 21, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28109204/aquaspirillum-soli-sp-nov-isolated-from-a-soil-sample-in-the-people-s-republic-of-china
#6
Gabriela Moya, Huan Trinh, Zheng-Fei Yan, KyungHwa Won, Jung-Eun Yang, Qi-Jun Wang, MooChang Kook, Tae-Hoo Yi
A Gram-stain-negative, smooth, opaque, white pigmented, helical-shaped and motile bacterium by means of bipolar tufts of flagella, catalase- and oxidase-positive, grew under microaerophilic conditions, was isolated from soil sample of a reed pond in Shangqiu, Henan province, P. R. China. The strain named THG-SQE6T grows well at 25-42 oC, pH 6.5-7.5 and in the presence of 0-1 % (w/v) NaCl. The phylogenetic analysis based on 16S rRNA gene sequence showed that the strain THG-SQE6T was most closely related to Aquaspirillum serpens IAM 13944T (97...
January 19, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28109203/pedobacter-lusitanus-sp-nov-a-new-bacterial-species-isolated-from-sludge-of-a-deactivated-uranium-mine-in-portugal
#7
Cláudia Covas, Tânia Caetano, Andreia Cruz, Tiago Santos, Liliana Dias, Guenter Klein, Amir Abdulmawjood, Luiz Rodríguez-Alcalá, Lígia Pimentel, Ana Gomes, Ana Cristina Freitas, Alba Garcia-Serrano, Javier Fontecha, Sónia Mendo
Strain NL19T is a Gram negative aerobic bacterium that was isolated from sludge of a deactivated uranium mine in Portugal. 16S rRNA gene sequence analysis revealed that strain NL19T is a member of the genus Pedobacter and closely related to the strains Pedobacter himalayensis MTCC 6384T, Pedobacter cryoconitis DSM 14825T, Pedobacter westerhofensis DSM 19036T and P. hartonius DSM 19033T. It has a DNA G+C content of 40.8 mol%, which agreed with the genus description. The main fatty acids include C16:1 9c, C14:1 9c, C4:0, Iso-C17:0, Iso-C17:0 3-OH, C16:0, Anteiso-C15:0 and Iso-C15:0 3-OH...
January 21, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28109202/algoriphagus-resistens-sp-nov-isolated-from-marine-sediment
#8
Ji-Ru Han, Jin-Xin Zhao, Zong-Jie Wang, Guan-Jun Chen, Zong-Jun Du
Strain NH1T, a pink-pigmented, facultatively anaerobic, heterotrophic, catalase-positive and oxidase-negative, Gram-stain-negative marine bacterium, was isolated from marine sediment in the coast of Weihai, China. Cells of strain NH1T was rod-shaped, 0.8-2.0 µm in length and 0.5 µm in width. The strain able to grow at 13-37 °C, pH 5.5-8.5, in the presence of 0.0-8.0% (w/v) NaCl. Optimal growth was observed at 28 °C, with 3.0% (w/v) NaCl and pH 6.5-7.0. Nitrate was reduced. The G+C content of the DNA was 41...
January 19, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28109200/arcobacter-lekithochrous-sp-nov-a-new-species-isolated-from-a-molluscan-hatchery-in-norway
#9
Ana L Diéguez, Sabela Balboa, Thorolf Magnesen, Jesús L Romalde
Four bacterial strains, LFT 1.7T, LT2C 2.5, LT4C 2.8 and TM 4.6, were isolated from Great scallop (Pecten maximus) larvae and tank seawater in a Norwegian hatchery and characterized by a polyphasic approach including phenotypic, chemotaxonomic and genomic traits. All of them were Gram-negative and motile rods, oxidase and catalase positive and required sea salts for growth. Major fatty acids present were Summed Feature 3 (C16:1ω7c/C16:1 ω6c), Summed Feature 8 (C18:1ω7c or C18:1 ω6c), C16:0, C14:0, Summed Feature 2 (C14:0 3OH/C16:1 iso I), C12:0 3OH and C12:0...
January 19, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28109187/antimicrobial-and-antioxidant-activities-of-saccharomyces-cerevisiae-ifst062013-a-potential-probiotic
#10
Md Fakruddin, Md Nur Hossain, Monzur Morshed Ahmed
BACKGROUND: Probiotic yeast has become a field of interest to scientists in recent years. METHODS: Conventional cultural method was employed to isolate and identify yeast and standard methods were used to determine different probiotic attributes, antimicrobial and antioxidant properties. RESULTS: This study reports potential probiotic properties of a strain of S. cerevisiae IFST 062013 isolated from fruit. The isolate is tolerant to a wide range of temperature and pH, high concentration of bile salt and NaCl, gastric juice, intestinal environment, α-amylase, trypsin and lysozyme...
January 21, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28109162/odontoblastic-differentiation-of-dental-pulp-stem-cells-from-healthy-and-carious-teeth-on-an-original-pcl-based-3d-scaffold
#11
A Louvrier, E Euvrard, L Nicod, G Rolin, F Gindraux, L Pazart, C Houdayer, P Y Risold, F Meyer, C Meyer
AIMS: To isolate and characterize Dental Pulp Stem Cells (DPSCs) obtained from carious and healthy mature teeth extracted when conservative treatment was not possible or for orthodontic reasons; to evaluate the ability of DPSCs to colonize, proliferate, and differentiate into functional odontoblast-like cells when cultured onto a polycaprolactone cone made by jet-spraying and prototyped into a design similar to a gutta-percha cone. METHODOLOGY: DPSCs were obtained from 9 carious and 12 healthy mature teeth...
January 21, 2017: International Endodontic Journal
https://www.readbyqxmd.com/read/28109150/-isolation-culture-and-identification-of-adipose-derived-stem-cells-from-sd-rat-adipose-tissues-subjected-to-long-term-cryopreservation
#12
Qin Liu, Liping Wang, Fang Chen, Yi Zhang
Objective To study the feasibility of isolation and culture of adipose-derived stem cells (ADSCs) from SD rat adipose tissues subjected to long-term cryopreservation. Methods We took inguinal fat pads from healthy SD rats. Adipose tissues were stored with 100 mL/L dimethyl sulfoxide (DMSO) combined with 900 mL/L fetal bovine serum (FBS) in liquid nitrogen. Three months later, the adipose tissues were resuscitated for the isolation and culture of ADSCs. The growth status and morphology were observed. The growth curve and cell surface markers CD29, CD45, CD90 of the 3rd passage cells were analyzed respectively by CCK-8 assay and immunocytochemistry...
February 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/28109149/-indirect-coculture-of-human-infrapatellar-fat-pad-derived-stem-cells-with-osteoarthritic-chondrocytes-induces-their-chondrogenesis
#13
Junjun Yang, Cheng Chen, Liu Yang, Xiongbo Song, Wenbin Xie, Shu Huang, Baorong Liu
Objective To promote phenotype recovery of osteoarthritic articular chondrocytes (OACs) and induce chondrogenic differentiation of infrapatellar fat pad-derived stem cells (IPFPSCs) by indirectly coculturing these two types of cells. Methods The OACs and IPFPSCs were isolated and cultured in vitro. This experiment included single IPFPSCs group, single OACs group, and coculture group. After cells were cultured in vitro with chondrogenic medium for 21 days, the chondrocyte phenotypes were determined by HE staining (cell morphology), Alcian blue staining (glycosaminoglycan content) and immunofluorescence cytochemistry (collagen 1, collagen 2, collagen 3, aggrecan, SOX9)...
February 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/28109148/-rat-bone-marrow-mesenchymal-stem-cells-promote-apoptosis-and-inhibit-proliferation-and-migration-of-rsc96-cells
#14
Yongbin Ma, Suohua Ge, Dan Zhou, Huanyan Zhang, Luyang Sun, Xuefeng Wang, Jianhua Su
Objective To investigate the effect of rat bone marrow mesenchymal stem cells (BMSCs) on the proliferation and migration of rat RSC96 Schwann cells in vitro. Methods BMSCs of Sprague Dawley (SD) rats were isolated by bone marrow cell adherent method. The morphology of the third generation of BMSCs was observed by inverted microscopy. The cell surface antigen makers CD19, CD34, CD73, CD105 were detected by flow cytometry. The third-passage BMSCs differentiated into osteoblasts and adipocytes after exposed to bone-inducing and lipid-inducing media for 3 and 2 weeks, respectively, and then were identified by oil red O and alkaline phosphatase staining...
February 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/28109139/-imiquimod-combined-with-dendritic-cell-vaccine-decreases-treg-proportion-and-enhances-anti-tumor-responses-in-mice-bearing-melanoma
#15
Shurong Ren, Qiubo Wang, Yanli Zhang, Cuixiu Lu, Ping Li, Yumei Li
Objective To investigate the therapeutic effect of Toll-like receptor 7 (TLR7) agonist imiquimod combined with dendritic cell (DC)-based tumor vaccine on melanoma in mice and the potential mechanism. Methods Melanoma-bearing mouse models were established by subcutanous injection of B16-OVA cells into C57BL/6 mice. DCs were isolated from mouse bone marrow and propagated in culture medium with recombinant mouse granulocyte-macrophage colony-stimulating factor (rmGM-CSF) and recombinant mouse interleukin-4 (rmIL-4)...
February 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/28109023/intraportal-islet-transplantation-the-impact-of-the-liver-micro-environment
#16
REVIEW
Vaihere Delaune, Thierry Berney, Stéphanie Lacotte, Christian Toso
The portal vein remains the preferred site for pancreatic islet transplantation due to its easy access and low morbidity. However, despite great progress in isolation and transplantation protocols over the past few years, it is still associated with the early loss of some 50-70% of transplanted islets. The complex liver micro-environment itself presumably plays an important role in this loss. The present review focuses on the specifics of the liver micro-environment, notably the localized hepatic ischemia/reperfusion injury following transplantation, the low oxygenation of the portal vein, the instant blood mediated inflammatory reaction (IBMIR), the endogenous liver immune system, and the gut-liver axis, and how they can each have an impact on the transplanted islets...
January 21, 2017: Transplant International: Official Journal of the European Society for Organ Transplantation
https://www.readbyqxmd.com/read/28109008/inhibition-of-group-1-p21-activated-kinases-suppresses-pancreatic-stellate-cell-activation-and-increases-survival-of-mice-with-pancreatic-cancer
#17
Dannel Yeo, Phoebe Phillips, Graham S Baldwin, Hong He, Mehrdad Nikfarjam
Pancreatic cancer remains one of the most lethal of all solid tumours. Pancreatic stellate cells (PSCs) are primarily responsible for the fibrosis that constitutes the stroma and p21-activated kinase 1 (PAK1) may have a role in signalling pathways involving PSCs. This study aimed to examine the role of PAK1 in PSCs and in the interaction of PSCs with pancreatic cancer cells. Human PSCs were isolated using a modified outgrowth method. The effect of inhibiting PAK1 with a group 1 PAK inhibitor, FRAX597, on cell proliferation and apoptosis in vitro was measured by thymidine incorporation and annexin V assays, respectively...
January 21, 2017: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/28108964/heme-oxygenase-1-defects-lead-to-reduced-chlorophyll-in-brassica-napus
#18
Lixia Zhu, Zonghui Yang, Xinhua Zeng, Jie Gao, Jie Liu, Bin Yi, Chaozhi Ma, Jinxiong Shen, Jinxing Tu, Tingdong Fu, Jing Wen
We previously described a Brassica napus chlorophyll-deficient mutant (ygl) with yellow-green seedling leaves and mapped the related gene, BnaC.YGL, to a 0.35 cM region. However, the molecular mechanisms involved in this chlorophyll defect are still unknown. In this study, the BnaC07.HO1 gene (equivalent to BnaC.YGL) was isolated by the candidate gene approach, and its function was confirmed by genetic complementation. Comparative sequencing analysis suggested that BnaC07.HO1 was lost in the mutant, while a long noncoding-RNA was inserted into the promoter of the homologous gene BnaA07...
January 20, 2017: Plant Molecular Biology
https://www.readbyqxmd.com/read/28108920/biochemical-and-physiological-responses-of-halophilic-nanophytoplankton-dunaliella-salina-from-exposure-to-xeno-estrogen-17%C3%AE-ethinylestradiol
#19
Dalel Belhaj, Khaled Athmouni, Doniez Frikha, Monem Kallel, Abdelfattah El Feki, Sami Maalej, John L Zhou, Habib Ayadi
The environmental impacts of various pollutants on the entire levels of organisms are under investigation. Among these pollutants, endocrine-disrupting compounds (EDCs) present a serious hazard, even though the environmental significance of these compounds remains basically unknown. To drop some light on this field, we assessed the effects of a 11-day exposure of 17α-ethinylestradiol (EE2) on the growth, metabolic content, antioxidant response, oxidative stress, and genetic damage of Dunaliella salina, isolated from Tunisian biotopes...
January 20, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28108874/surviving-in-isolation-genetic-variation-bottlenecks-and-reproductive-strategies-in-the-canarian-endemic-limonium-macrophyllum-plumbaginaceae
#20
Ares Jiménez, Barbara Weigelt, Arnoldo Santos-Guerra, Juli Caujapé-Castells, José María Fernández-Palacios, Elena Conti
Oceanic archipelagos are typically rich in endemic taxa, because they offer ideal conditions for diversification and speciation in isolation. One of the most remarkable evolutionary radiations on the Canary Islands comprises the 16 species included in Limonium subsection Nobiles, all of which are subject to diverse threats, and legally protected. Since many of them are single-island endemics limited to one or a few populations, there exists a risk that a loss of genetic variation might limit their long-term survival...
January 20, 2017: Genetica
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