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https://www.readbyqxmd.com/read/29332347/genetically-engineered-bone-marrow-derived-mesenchymal-stem-cells-co-expressing-ifn-%C3%AE-and-il-10-inhibit-hepatocellular-carcinoma-by-modulating-mapk-pathway
#1
Hao Wang, Jun Wang, Xiaolei Shi, Yitao Ding
PURPOSE: One of the major challenges in delivering cytokines for the treatment of hepatocellular carcinoma (HCC) is the mode of delivery. This study hypothesized that genetically engineered bone marrow derived mesenchymal stem cells (BMSCs) co-expressing IFN-γ and IL-10 can serve as a potential therapeutic strategy in the treatment of HCC by inhibiting cell proliferation. METHODS: Male Sprague-Dawley rats (n=5, 200-250 g) for BMSCs isolation and Nude/SCID mice (n=35,12-20g) to develop liver cancer xenograft model were used...
November 2017: Journal of B.U.ON.: Official Journal of the Balkan Union of Oncology
https://www.readbyqxmd.com/read/29332290/dna-diffusion-assay-applied-to-plant-cells
#2
Anca Macovei, Mattia Donà, Daniela Carbonera, Alma Balestrazzi
DNA diffusion assay is a simple, sensitive and reliable technique which allows the assessment of programmed cell death (PCD) or necrosis events based on nuclear morphology. It consists in isolating nuclei from plant material, which are then embedded in agarose and subjected to lysis in alkaline buffers. Under these conditions, and due to the presence of abundant alkali-labile sites in the DNA, small pieces of DNA diffuse in the agarose gel giving a specific halo appearance when stained with fluorescent dyes like DAPI (4',6-diamidino-2-phenylindole)...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29332286/analysis-of-mitochondrial-markers-of-programmed-cell-death
#3
Dong Xiao, Huyi He, Wenjing Huang, Thet Lwin Oo, Aiqin Wang, Long-Fei He
Mitochondria play a crucial role in programmed cell death (PCD) in plants. In most cases of mitochondria-dependent PCD, cytochrome c (Cyt c) released from mitochondria due to the opening of mitochondrial permeability transition pore (MPTP) and the activation of caspase-like proteases. Here we describe the analytic methods of mitochondrial markers of PCD including mitochondria isolation, mitochondrial membrane permeability, mitochondrial inner membrane potential, Cytc release, ATP, and mitochondrial reactive oxygen species (ROS)...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29332277/efficient-degradation-of-triclosan-by-an-endophytic-fungus-penicillium-oxalicum-b4
#4
Hao Tian, Yan Jun Ma, Wan Yi Li, Jian Wen Wang
Triclosan (TCS), a widely used antimicrobial and preservative agent, is an emerging contaminant in aqueous and soil environment. Microbial degradation of TCS has not been reported frequently because of its inhibition of microbe growth. To explore the new microbial resources for TCS biodegradation, fungal endophytes were isolated and screened for the degradation potential. The endophytic strain B4 isolated from Artemisia annua L. showed higher degradation efficiency and was identified as Penicillium oxalicum based on its morphology and ITS sequences of ribosomal DNA...
January 13, 2018: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29332194/the-active-glycosides-from-urtica-fissa-rhizome-decoction
#5
Mengyue Wang, Ying Zhang, Hao Zhang, Xiaoru Feng, Xiaobo Li
Using bioassay guided fractionation, 16 glycosides, including two new compounds (1 and 2), were isolated from the anticomplement and anti-inflammatory portion of an Urtica fissa rhizome decoction used for arthritis. Several compounds were found to possess significant anticomplement and anti-inflammatory activities. This study revealed that glycosides played an important role in the therapeutic effects of Urtica fissa rhizome.
January 13, 2018: Journal of Natural Medicines
https://www.readbyqxmd.com/read/29332193/melanogenesis-inhibitory-activity-of-components-from-salam-leaf-syzygium-polyanthum-extract
#6
Andriyana Setyawati, Kae Hirabayashi, Kosei Yamauchi, Hiroyuki Hattori, Tohru Mitsunaga, Irmanida Batubara, Rudi Heryanto, Hiroshi Hashimoto, Mitsuyuki Hotta
In order to identify a novel whitening agent, the methanol extract of S. polyanthum leaf was focused on by the screening test using nine Indonesian medicinal plants for the inhibition of melanogenesis and tyrosinase activity in B16 melanoma cells. Three novel compounds [(1) 1-(2,3,5-trihydroxy-4-methylphenyl)hexane-1-one, (2) 1-(2,3,5-trihydroxy methylphenyl)octane-1-one, and (3) (4E)-1-(2,3,5-trihydroxy-4-methylphenyl)decan-1-one and one known compound [(4) 1-(2,3,5-trihydroxy-4-methylphenyl)decan-1-one were isolated from the methanol extract...
January 13, 2018: Journal of Natural Medicines
https://www.readbyqxmd.com/read/29332191/genome-wide-characterization-of-differentially-expressed-genes-provides-insights-into-regulatory-network-of-heat-stress-response-in-radish-raphanus-sativus-l
#7
Ronghua Wang, Yi Mei, Liang Xu, Xianwen Zhu, Yan Wang, Jun Guo, Liwang Liu
Heat stress (HS) causes detrimental effects on plant morphology, physiology, and biochemistry that lead to drastic reduction in plant biomass production and economic yield worldwide. To date, little is known about HS-responsive genes involved in thermotolerance mechanism in radish. In this study, a total of 6600 differentially expressed genes (DEGs) from the control and Heat24 cDNA libraries of radish were isolated by high-throughput sequencing. With Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, some genes including MAPK, DREB, ERF, AP2, GST, Hsf, and Hsp were predominantly assigned in signal transductions, metabolic pathways, and biosynthesis and abiotic stress-responsive pathways...
January 13, 2018: Functional & Integrative Genomics
https://www.readbyqxmd.com/read/29332155/prevalence-of-the-pfdhfr-and-pfdhps-mutations-among-asymptomatic-pregnant-women-in-southeast-nigeria
#8
Ekpereonne Esu, Costanza Tacoli, Prabhanjan Gai, Nicole Berens-Riha, Michael Pritsch, Thomas Loescher, Martin Meremikwu
Sulfadoxine-pyrimethamine (SP) is the recommended drug for intermittent preventive treatment of malaria in pregnancy in most of sub-Saharan Africa. Resistance to SP is related to mutations in the dhfr and dhps gene of Plasmodium falciparum. This study determined the prevalence of Pfdhfr and Pfdhps polymorphisms found in asymptomatic pregnant women attending antenatal care in Calabar, Nigeria. From October 2013 to November 2014, asymptomatic pregnant women attending antenatal care clinics were enrolled after obtaining informed consent...
January 13, 2018: Parasitology Research
https://www.readbyqxmd.com/read/29332154/the-influence-of-systemically-or-locally-administered-mesenchymal-stem-cells-on-tissue-repair-in-a-rat-oral-implantation-model
#9
Miya Kanazawa, Ikiru Atsuta, Yasunori Ayukawa, Takayoshi Yamaza, Ryosuke Kondo, Yuri Matsuura, Kiyoshi Koyano
BACKGROUND: Multipotent mesenchymal stem cells (MSCs) are used clinically in regenerative medicine. Our previous report showed systemically injected MSCs improved peri-implant sealing and accelerated tissue healing. However, the risks of systemic MSC administration, including lung embolism, must be considered; therefore, their local application must be assessed for clinical safety and efficacy. We investigated differences in treatment effect between local and systemic MSC application using a rat oral implantation model...
January 13, 2018: International Journal of Implant Dentistry
https://www.readbyqxmd.com/read/29332142/purification-and-characterization-of-a-novel-organic-solvent-tolerant-and-cold-adapted-lipase-from-psychrobacter-sp-zy124
#10
Yue Zhang, Fangling Ji, Jingyun Wang, Zhongji Pu, Bo Jiang, Yongming Bao
By screening 25 different psychrophilic strains isolated from the Arctic habitat, we isolated a strain capable of producing lipase. We identified this strain as Psychrobacter sp. ZY124 based on the amplified 16S rDNA sequence. The lipase, named as Lipase ZC12, produced from the supernatant of Psychrobacter sp. ZY124 cultured at 15 °C was purified to homogeneity by ammonium sulfate precipitation followed by Phenyl Sepharose FF gel hydrophobic chromatography. Based on the obtained amino acid sequence, Lipase ZC12 is classified as a member of the Proteus/psychrophilic subfamily of lipase family I...
January 13, 2018: Extremophiles: Life Under Extreme Conditions
https://www.readbyqxmd.com/read/29332141/cultivable-fungi-present-in-antarctic-soils-taxonomy-phylogeny-diversity-and-bioprospecting-of-antiparasitic-and-herbicidal-metabolites
#11
Eldon C Q Gomes, Valéria M Godinho, Débora A S Silva, Maria T R de Paula, Gislaine A Vitoreli, Carlos L Zani, Tânia M A Alves, Policarpo A S Junior, Silvane M F Murta, Emerson C Barbosa, Jaquelline G Oliveira, Fabio S Oliveira, Camila R Carvalho, Mariana C Ferreira, Carlos A Rosa, Luiz H Rosa
Molecular biology techniques were used to identify 218 fungi from soil samples collected from four islands of Antarctica. These consisted of 22 taxa of 15 different genera belonging to the Zygomycota, Ascomycota, and Basidiomycota. Mortierella, Antarctomyces, Pseudogymnoascus, and Penicillium were the most frequently isolated genera and Penicillium tardochrysogenum, Penicillium verrucosus, Goffeauzyma gilvescens, and Mortierella sp. 2 the most abundant taxa. All fungal isolates were cultivated using solid-state fermentation to obtain their crude extracts...
January 13, 2018: Extremophiles: Life Under Extreme Conditions
https://www.readbyqxmd.com/read/29332105/the-emergence-of-carbapenem-resistant-klebsiella-pneumoniae-isolates-producing-oxa-48-and-ndm-in-the-southern-asir-province-saudi-arabia
#12
Ibrahim A Al-Zahrani, Bander A Alsiri
To identify the prevalence of carbapenem-resistant Klebsiella pneumoniae (CRKP) and the most common types of cabapenemases among CRKP in the Southern (Asir) province hospitals, Saudi Arabia. Methods: The cross-sectional study was conducted between late April and September in 2015. A total of 54 Klebsiella pneumoniae (K. pneumoniae) isolates with reduced sensitivity to carbapenems were obtained from various clinical specimens of the 2 largest hospitals in the Southern province. Minimum inhibitory concentrations (MICs) of carbapenems were confirmed using E-test...
January 2018: Saudi Medical Journal
https://www.readbyqxmd.com/read/29332080/radiosurgical-pallidotomy-for-parkinson-s-disease
#13
Leslie D Cahan, Ronald F Young, Francisco Li
Deep brain stimulation (DBS) has been widely accepted as a tool for treating many symptoms of Parkinson's disease (PD); pallidotomy has been nearly abandoned. Concerns about both the safety and efficacy of pallidotomy are based on small series, isolated case reports, and techniques that would now be considered obsolete. The senior author recently reviewed long-term follow-up of a series of patients who had gamma knife pallidotomy (GKP) for advanced PD. GKP leads to durable, clinically significant benefit. Bilateral GKP adds incremental improvement...
2018: Progress in Neurological Surgery
https://www.readbyqxmd.com/read/29331959/probenecid-improves-cardiac-function-in-patients-with-heart-failure-with-reduced-ejection-fraction-in-vivo-and-cardiomyocyte-calcium-sensitivity-in-vitro
#14
Nathan Robbins, Mark Gilbert, Mohit Kumar, James W McNamara, Patrick Daly, Sheryl E Koch, Ginger Conway, Mohamed Effat, Jessica G Woo, Sakthivel Sadayappan, Jack Rubinstein
BACKGROUND: Transient receptor potential vanilloid 2 is a calcium channel activated by probenecid. Probenecid is a Food and Drug Administration-approved uricosuric drug that has recently been shown to induce positive lusitropic and inotropic effects in animal models through cardiomyocyte transient receptor potential vanilloid 2 activation. The aim of this study was to test the hypothesis that oral probenecid can improve cardiac function and symptomatology in patients with heart failure with reduced ejection fraction and to further elucidate its calcium-dependent effects on myocyte contractility...
January 13, 2018: Journal of the American Heart Association
https://www.readbyqxmd.com/read/29331937/quasi-static-tensile-properties-of-the-cranial-cruciate-ligament-crcl-in-adult-cattle-towards-the-design-of-a-prosthetic-crcl
#15
Lucien Diotalevi, Yvan Petit, Vladimir Brailovski, Sylvain Nichols, Emma Marchionatti, Éric Wagnac
Mechanical properties of the Cranial Cruciate Ligament (CrCL) in adult cattle are not well documented and protocols used in the literature focus on testing a full femur-CrCL-tibia complex rather than an isolated CrCL. The aim of this study was to assess a wider range of tensile properties of the CrCL along its anatomic axis with experimental measurements of the global elongation, displacement and strain fields, in order to provide guidelines for the design of CrCL prosthetic surrogates. Fourteen bovine CrCL were harvested from seven mature cows (5...
December 26, 2017: Journal of the Mechanical Behavior of Biomedical Materials
https://www.readbyqxmd.com/read/29331925/overexpression-of-chrysanthemum-lavandulifolium-clcbf1-in-chrysanthemum-morifolium-white-snow-improves-the-level-of-salinity-and-drought-tolerance
#16
Wenjie Gao, Miao He, Jie Liu, Xin Ma, Yu Zhang, Silan Dai, Yunwei Zhou
This paper reports the first study on plant CBF transcription factors (TF) in salt and drought stress responses in Chrysanthemum lavandulifolium. A CBF homolog gene, named as ClCBF1, from C. lavandulifolium was isolated using rapid amplification of cDNA ends (RACE). The deduced peptide is comprised of 210 amino acids (AA) containing an AP2 structural domain characteristic of the AP2 gene family. Quantitative real-time PCR revealed that ClCBF1 gene exhibit differential expression patterns across root, leaf and stem tissues, and it was strongly induced under salt and drought treatments of C...
January 8, 2018: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/29331921/cyclotron-production-of-99mtc-comparison-of-known-separation-technologies-for-isolation-of-99mtc-from-molybdenum-targets
#17
REVIEW
Magdalena Gumiela
Intensive efforts were undertaken during the last few decades for the separation of cyclotron-produced 99mTc from 99Mo and new papers have been published on this topic since the last review [1]. In the future the cyclotron-based methods can replace reactor-based technology in producing this medical radioisotope and the nuclear reaction 100Mo(p,2n)99mTc appears to be the most worthwhile approach. New ways of producing of 99mTc require efficient separation methods. Several strategies for separation of 99mTc from 99Mo have been already developed...
November 10, 2017: Nuclear Medicine and Biology
https://www.readbyqxmd.com/read/29331903/rapid-structure-prediction-by-hplc-esi-msn-of-twenty-five-polyoxypregnane-tetraglycosides-from-dregea-sinensis-with-nmr-confirmation-of-eight-structures
#18
Juan Song, Rongji Dai, Yulin Deng, Fang Lv
Dregea sinensis Hemsl is an important herbal medicine in the Dai nationality of China. Its prominent clinical application has generated interest in the polyoxypregnane glycosides of the plant. This paper describes an extension of previous research on the polyoxypregnane di- and triglycosides of D. sinensis, aiming at identifying related tetraglycosides. On the basis of HPLC-ESI-MSn analysis in positive mode, twenty-five previously undescribed polyoxypregnane tetraglycosides were characterized (regarding molecular masses and fragmentation in MSn) from an ethyl acetate fraction that was not previously investigated...
January 11, 2018: Phytochemistry
https://www.readbyqxmd.com/read/29331877/cerebral-changes-and-disrupted-gray-matter-cortical-networks-in-asymptomatic-older-adults-at-risk-for-alzheimer-s-disease
#19
Jose L Cantero, Mercedes Atienza, Pascual Sanchez-Juan, Eloy Rodriguez-Rodriguez, Jose Luis Vazquez-Higuera, Ana Pozueta, Andrea Gonzalez-Suarez, Eduard Vilaplana, Jordi Pegueroles, Victor Montal, Rafael Blesa, Daniel Alcolea, Alberto Lleo, Juan Fortea
The diagnostic value of cerebrospinal fluid (CSF) biomarkers is well established in Alzheimer's disease, but our current knowledge about how abnormal CSF levels affect cerebral integrity, at local and network levels, is incomplete in asymptomatic older adults. Here, we have collected CSF samples and performed structural magnetic resonance imaging scans in cognitively normal elderly as part of a cross-sectional multicenter study (SIGNAL project). To identify group differences in cortical thickness, white matter volume, and properties of structural networks, participants were split into controls (N = 20), positive amyloid-β (Aβ1-42+) (N = 19), and positive phosphorylated tau (N = 18)...
December 20, 2017: Neurobiology of Aging
https://www.readbyqxmd.com/read/29331867/classification-and-evolution-of-human-papillomavirus-genome-variants-alpha-5-hpv26-51-69-82-alpha-6-hpv30-53-56-66-alpha-11-hpv34-73-alpha-13-hpv54-and-alpha-3-hpv61
#20
Zigui Chen, Mark Schiffman, Rolando Herrero, Rob DeSalle, Kathryn Anastos, Michel Segondy, Vikrant V Sahasrabuddhe, Patti E Gravitt, Ann W Hsing, Paul K S Chan, Robert D Burk
HPV variants from the same type can be classified into lineages and sublineages based on the complete genome differences and the phylogenetic topologies. We examined nucleotide variations of twelve HPV types within the species Alpha-5 (HPV26, 51, 69, 82), Alpha-6 (HPV30, 53, 56, 66), Alpha-11 (HPV34, 73), Alpha-13 (HPV54) and Alpha-3 (HPV61) by analyzing 1432 partial sequences and 181 complete genomes from multiple geographic populations. The inter-lineage and inter-sublineage mean differences of HPV variants ranged between 0...
January 11, 2018: Virology
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