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https://www.readbyqxmd.com/read/28641406/novel-antimicrobial-activities-of-a-peptide-derived-from-a-japanese-soybean-fermented-food-natto-against-streptococcus-pneumoniae-and-bacillus-subtilis-group-strains
#1
Manabu Kitagawa, Tsukasa Shiraishi, Soh Yamamoto, Ryosuke Kutomi, Yasuo Ohkoshi, Toyotaka Sato, Hideki Wakui, Hideaki Itoh, Atsushi Miyamoto, Shin-Ichi Yokota
We recently isolated a tumoricidal peptide from Natto, a Japanese traditional fermented food. In the present study, antimicrobial activity of the Natto peptide was examined. The peptide consisted of 45 amino acid residues, and its structure was predicted to be rich in α-helix. It excreted antimicrobial activity only against Streptococcus pneumoniae and Bacillus subtilis group (B. subtilis, Bacillus pumilus, and Bacillus licheniformis). Lesser antimicrobial activity was observed for Streptococcus species other than S...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28641403/a-molasses-habitat-derived-fungus-aspergillus-tubingensis-xg21-with-high-%C3%AE-fructofuranosidase-activity-and-its-potential-use-for-fructooligosaccharides-production
#2
Yijia Xie, Huanxia Zhou, Caixia Liu, Jing Zhang, Ning Li, Zhanli Zhao, Guoyong Sun, Yaohua Zhong
The industrial microorganisms used for fructooligosaccharides (FOS) synthesis are generally fermented with sucrose as carbon source to induce the production of β-fructofuranosidase (FFase) having transfructosylation activity. Consequently, isolation of novel FFase producers from a sucrose-enriched biotope would help improve FOS productivity and reduce the process cost. Here, three fungi isolated from a unique sugarcane molasses habitat were found to possess FFase activity and one of them, XG21, exhibited a high capacity to synthesize FOS...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28641392/alcohol-is-not-protective-against-mortality-following-traumatic-brain-injury
#3
Jennifer S Albrecht, Majid Afshar, Deborah M Stein, Gordon S Smith
Alcohol exposure results in reduced mortality following traumatic brain injury (TBI) in animal models, yet clinical studies reported conflicting results and clinical trials based on proposed mechanisms have been disappointing. Methodological issues common to many of the clinical studies may have contributed to spurious results. Our objective was to evaluate the association between blood alcohol concentration (BAC) and in-hospital mortality following TBI, overcoming methodological problems of prior studies. We conducted a retrospective cohort study on individuals treated for isolated TBI at the R Adams Cowley Shock Trauma Center between 1997-2012 (n = 1,084)...
June 21, 2017: American Journal of Epidemiology
https://www.readbyqxmd.com/read/28641286/effect-of-bexarotene-on-platelet-activation-and-apoptosis
#4
Hang Cao, Rosi Bissinger, Anja T Umbach, Meinrad Gawaz, Florian Lang
BACKGROUND/AIMS: The retinoid X receptor (RXRs) stimulator Bexarotene ((4-[1-(3,5,5,8,8-pentamethyl-5,6,7,8-tetrahydro-2-naphthyl)ethynyl] benzoic acid) is used for the treatment of several malignancies. Bexarotene is at least in part effective by stimulation of apoptosis of tumor cells. Moreover, Bexarotene triggers eryptosis, the suicidal death of erythrocytes. Similar to erythrocytes, blood platelets lack nuclei but are nevertheless able to enter an apoptosis-like phenotype, characterized by caspase activation, cell shrinkage and cell membrane scrambling with phospha-tidylserine translocation to the cell surface...
June 23, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28641162/triterpenoids-and-sterols-from-the-grains-of-echinochloa-utilis-ohwi-yabuno-and-their-cytotoxic-activity
#5
Thi Trang Nguyen, Duc Hung Nguyen, Bing Tian Zhao, Duc Dat Le, Byung Sun Min, Young Ho Kim, Mi Hee Woo
Two new tetracyclic triterpenoids, echinochlorins C (1) and D (2), and sawamilletin (3) with new spectroscopic data were isolated from Echinochloa utilis Ohwi & Yabuno grains, along with one known triterpenoid (4) and eight sterols (5-12). Their structures were elucidated by spectroscopic data analyses (IR, UV, MS, and NMR). These compounds were tested in vitro cytotoxic activities against the human tumor-cell lines (HeLa, HL-60, and MCF-7). Compounds 6 and 8 displayed potential cytotoxic activity against HeLa, with IC50 values of 3...
June 19, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28641152/urea-induced-ros-accelerate-senescence-in-endothelial-progenitor-cells
#6
Maria D'Apolito, Anna Laura Colia, Maria Lasalvia, Vito Capozzi, Maria Pia Falcone, Massimo Pettoello-Mantovani, Michael Brownlee, Angela Bruna Maffione, Ida Giardino
BACKGROUND AND AIMS: The pathogenic events responsible for the reduction of endothelial progenitor cell (EPC) number and function seen in patients with chronic renal failure (CRF) are poorly understood. Here we investigate the hypothesis that increased concentrations of urea associated with CRF increase ROS production directly in EPCs, causing abnormalities associated with coronary artery disease risk. METHODS: Human EPCs were isolated from peripheral blood mononuclear cells of healthy donors and cultured in the presence or absence of 20 mmol/L urea...
June 15, 2017: Atherosclerosis
https://www.readbyqxmd.com/read/28641144/impact-of-saccharomyces-cerevisiae-and-torulaspora-delbrueckii-starter-cultures-on-cocoa-beans-fermentation
#7
Simonetta Visintin, Lacerda Ramos, Nara Batista, Paola Dolci, Freitas Schwan, Luca Cocolin
Aim of this work was to study the impact of mixed cultures of Saccharomyces cerevisiae and Torulaspora delbrueckii and T. delbrueckii monoculture on the fermentation process conducted on two different cocoa hybrids, PS1319 and SJ02, in Bahia, Brazil. This was performed throughout studying physico-chemical changes during the fermentation process and analyzing volatile compounds and sensory analysis of chocolates. (GTG)5-PCR fingerprinting was used to type isolates at strain level allowing to assess the implantation of the starter cultures added...
June 7, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28641051/beneficial-role-of-citrus-fruit-polyphenols-against-hepatic-dysfunctions-a-review
#8
Md Mohabbulla Mohib, Kazi Afnan, Tasfiq Zaman Paran, Salma Khan, Juthika Sarker, Nahid Hasan, Istiaque Hasan, Md Abu Taher Sagor
Alcoholic liver diseases and virus-induced hepatic dysfunctions are prevalent in western countries. Evidence also suggests that hyperglycemia and insulin resistance are key players in the development of hepatic diseases and their complications. Since the comorbid diseases like obesity, diabetes and vascular dysfunctions primarily affect liver, the modern therapies against other hepatic dysfunctions are becoming a major challenge to treat. In addition to these, polypharmacy and adverse drug reactions (ADRs) are further aggravating the phenomenon...
June 22, 2017: Journal of Dietary Supplements
https://www.readbyqxmd.com/read/28641033/evaluation-of-planned-bicanalicular-nasolacrimal-tube-retention-in-treating-punctal-stenosis-or-occlusion-secondary-to-trachoma
#9
Rania A Ahmed, Ahmed M Kamal, Mohamed A Zayed
This article evaluates retaining bicanalicular silicone nasolacrimal tube in patients susceptible to restenosis after punctoplasty. Sixty-nine eyes (38 patients) suffering from epiphora due to stenosis or occlusion of both punctae were included in this prospective study. All had 3 snip punctoplasty and bicanalicular silicone nasolacrimal duct insertion. Cases associated with proximal canalicular stenosis or obstructions were excluded. Tolerance to tube presence until the time of loss or removal was evaluated using a score (0 to 2)...
June 22, 2017: Orbit
https://www.readbyqxmd.com/read/28641017/nanpdb-a-resource-for-natural-products-from-northern-african-sources
#10
Fidele Ntie-Kang, Kiran K Telukunta, Kersten Döring, Conrad V Simoben, Aurélien F A Moumbock, Yvette I Malange, Leonel E Njume, Joseph N Yong, Wolfgang Sippl, Stefan Günther
Natural products (NPs) are often regarded as sources of drugs or drug leads or simply as a "source of inspiration" for the discovery of novel drugs. We have built the Northern African Natural Products Database (NANPDB) by collecting information on ∼4500 NPs, covering literature data for the period from 1962 to 2016. The data cover compounds isolated mainly from plants, with contributions from some endophyte, animal (e.g., coral), fungal, and bacterial sources. The compounds were identified from 617 source species, belonging to 146 families...
June 22, 2017: Journal of Natural Products
https://www.readbyqxmd.com/read/28641008/hypoxia-induced-changes-in-the-fibroblast-secretome-exosome-and-whole-cell-proteome-using-cultured-cardiac-derived-cells-isolated-from-neonatal-mice
#11
Jake Cosme, Hongbo Guo, Sina Hadipour-Lakmehsari, Andrew Emili, Anthony O Gramolini
Cardiac fibroblasts (CF) represent a major subpopulation of cells in the developing and adult heart. Cardiomyocyte (CM) and CF intercellular communication occurs through paracrine interactions and modulate myocyte development and stress response. Detailed proteomic analysis of the CF secretome in normal and stressed conditions may offer insights into the role of CF in heart development and disease. Primary neonatal mouse CF were isolated and cultured for 24h in 21% (normoxic) or 2% (hypoxic) O2. Conditioned media was separated to obtain EXO and EXO-depleted secretome fractions...
June 22, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28640968/the-impact-of-andean-patagonian-mycoflora-in-the-search-for-new-lead-molecules
#12
REVIEW
Pedro M Aqueveque, Carlos L Cespedes, Isao Kubo, David S Seigler, Olov Sterner
Secondary metabolites from fungi have become a major source of chemical innovation in programs searching for lead molecules with bioactivities, especially over the last 50 years. In this review, we discuss the fundamental considerations in the discovery of molecules for agricultural and medicinal uses. This group of organisms possesses a strong potential for scientific and industrial communities. Recently, the incorporation of new technologies for the artificial cultivation of fungi and the use of better equipment to isolate and identify active metabolites has allowed the discovery of leading molecules for the design of new and safer drugs and pesticides...
June 22, 2017: Annals of the New York Academy of Sciences
https://www.readbyqxmd.com/read/28640957/restoration-of-cutaneous-pigmentation-by-transplantation-to-mice-of-isogeneic-human-melanocytes-in-dermal-epidermal-engineered-skin-substitutes
#13
Steven T Boyce, Christopher M Lloyd, Mark C Kleiner, Viki B Swope, Zalfa Abdel-Malek, Dorothy M Supp
Autologous engineered skin substitutes (ESS) containing melanocytes (hM) may restore pigmentation, and photoprotection after grafting to full-thickness skin wounds. In the current study, normal hM were isolated from discard skin, propagated with or without tyrosinase inhibitors, cryopreserved, recovered into culture, and added to ESS (ESS-P) before transplantation. ESS-P were incubated in either UCMC160/161 or UCDM1 medium, scored for hM densities, and grafted to mice. The results showed that: sufficient hM can be propagated to expand donor tissue by 100-fold; incubation of hM in tyrosinase inhibitors reduced pigment levels but did not change hM recovery after cryopreservation; hM densities in ESS-P were greater after incubation in UCDM1 than UCMC160 medium; hM were localized to the dermal-epidermal junction of ESS-P; and UCDM1 medium promoted earlier pigment distribution and density...
June 22, 2017: Pigment Cell & Melanoma Research
https://www.readbyqxmd.com/read/28640942/polyfunctional-response-by-immtac-imcgp100-redirected-cd8-and-cd4-t-cells
#14
Caroline Boudousquie, Giovanna Bossi, Jacob M Hurst, Karolina A Rygiel, Bent K Jakobsen, Namir J Hassan
The success of immune system based cancer therapies depends on a broad immune response engaging a range of effector cells and mechanisms. Immune mobilising monoclonal TCRs against cancer (ImmTAC(™) molecules, fusion proteins consisting of a soluble, affinity enhanced TCR and an anti-CD3 scFv Ab) were previously shown to redirect CD8(+) and CD4(+) T cells against tumours. Here we present evidence that IMCgp100 (ImmTAC recognising a peptide derived from the melanoma-specific protein, gp100, presented by HLA-A*0201) efficiently redirects and activates effector and memory cells from both CD8(+) and CD4(+) repertoires...
June 22, 2017: Immunology
https://www.readbyqxmd.com/read/28640922/changes-in-hemp-secondary-fiber-production-related-to-technical-fiber-variability-revealed-by-light-microscopy-and-attenuated-total-reflectance-fourier-transform-infrared-spectroscopy
#15
Eva Fernandez-Tendero, Arnaud Day, Sandrine Legros, Anouck Habrant, Simon Hawkins, Brigitte Chabbert
Interest in hemp (Cannabis sativa L.) is increasing due to the development of a new range of industrial applications based on bast fibers. However the variability of bast fiber yield and quality represents an important barrier to further exploitation. Primary and secondary fiber content was examined in two commercial hemp varieties (Fedora 17, Santhica 27) grown under contrasted sowing density and irrigation conditions. Both growing conditions and hemp varieties impact stem tissue architecture with a large effect on the proportion of secondary fibers but not primary fibers...
2017: PloS One
https://www.readbyqxmd.com/read/28640915/physiological-and-genomic-features-of-a-novel-violacein-producing-bacterium-isolated-from-surface-seawater
#16
Yue-Hong Wu, Hong Cheng, Lin Xu, Xiong-Bin Jin, Chun-Sheng Wang, Xue-Wei Xu
Strains JW1T and JW3, isolated from surface seawater of the Arabian Sea, were subjected to polyphasic taxonomic analysis. Cells of both strains were Gram-stain-negative, aerobic, and rod-shaped. They formed violet pigment and produced violacein. On the basis of 16S rRNA gene sequence analysis, strains JW1T and JW3 showed high 16S rRNA gene sequence similarity with Pseudoalteromonas byunsanensis JCM12483T (98.2%), P. shioyasakiensis SE3T (97.8%), P. arabiensis JCM 17292T (97.3%), and P. gelatinilytica NH153T (97...
2017: PloS One
https://www.readbyqxmd.com/read/28640892/novel-resistance-to-cydia-pomonella-granulovirus-cpgv-in-codling-moth-shows-autosomal-and-dominant-inheritance-and-confers-cross-resistance-to-different-cpgv-genome-groups
#17
Annette J Sauer, Eva Fritsch, Karin Undorf-Spahn, Petr Nguyen, Frantisek Marec, David G Heckel, Johannes A Jehle
Commercial Cydia pomonella granulovirus (CpGV) products have been successfully applied to control codling moth (CM) in organic and integrated fruit production for more than 30 years. Since 2005, resistance against the widely used isolate CpGV-M has been reported from different countries in Europe. The inheritance of this so-called type I resistance is dominant and linked to the Z chromosome. Recently, a second form (type II) of CpGV resistance in CM was reported from a field population (NRW-WE) in Germany. Type II resistance confers reduced susceptibility not only to CpGV-M but to most known CpGV isolates and it does not follow the previously described Z-linked inheritance of type I resistance...
2017: PloS One
https://www.readbyqxmd.com/read/28640885/phylogeography-of-a-tough-rock-survivor-in-european-dry-grasslands
#18
Daniela Listl, Peter Poschlod, Christoph Reisch
Phylogeographic analyses of plants in Europe have revealed common glacial refugia and migration routes for several trees and herbs with arctic-alpine distributions. The postglacial histories of dry grassland species in central Europe have rarely been analyzed, even though the extremely species-rich habitat is threatened. Sedum album (Crassulaceae) is a common inhabitant of rocky sites in central European dry grasslands. We inferred the phylogeographic history of S. album over its distribution range in Europe...
2017: PloS One
https://www.readbyqxmd.com/read/28640870/dominance-of-community-associated-methicillin-resistant-staphylococcus-aureus-clones-in-a-maternity-hospital
#19
Edet E Udo, Noura Al-Sweih
BACKGROUND: Methicillin- resistant Staphylococcus aureus (MRSA) is a major pathogen causing healthcare- and community- acquired infections. The purpose of this study was to characterize MRSA isolated at the Maternity Hospital between 2006 and 2011 for their genetic relatedness. MATERIALS AND METHODS: The MRSA isolates were investigated using a combination of antibiogram, Staphylococcal chromosome cassette mec (SCCmec) and spa typing to determine their relatedness to MRSA isolated in other Kuwait hospitals...
2017: PloS One
https://www.readbyqxmd.com/read/28640869/development-of-an-automated-size-based-filtration-system-for-isolation-of-circulating-tumor-cells-in-lung-cancer-patients
#20
Satomi Yagi, Yasuhiro Koh, Hiroaki Akamatsu, Kuninobu Kanai, Atsushi Hayata, Nahomi Tokudome, Keiichiro Akamatsu, Katsuya Endo, Seita Nakamura, Masayuki Higuchi, Hisashige Kanbara, Masanori Nakanishi, Hiroki Ueda, Nobuyuki Yamamoto
Circulating tumor cells (CTCs), defined as tumor cells circulating in the peripheral blood of patients with solid tumors, are relatively rare. Diagnosis using CTCs is expected to help in the decision-making for precision cancer medicine. We have developed an automated microcavity array (MCA) system to detect CTCs based on the differences in size and deformability between tumor cells and normal blood cells. Herein, we evaluated the system using blood samples from non-small-cell lung cancer (NSCLC) and small-cell lung cancer (SCLC) patients...
2017: PloS One
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