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https://www.readbyqxmd.com/read/27922236/microorganisms-isolated-from-cultures-and-infection-focus-and-antibiotic-treatments-in-febrile-neutropenic-children-from-%C3%A5-anl%C3%A4-urfa-turkey
#1
Z Canan Özdemir, Ahmet Koç, Ali Ayçiçek
Chemotherapy induced febrile neutropenia predisposes patients to life threatening infections. We aimed to determine the causative microorganisms, infection focus and antibiotic treatment success in febrile neutropenic children with leukemia. A total of 136 febrile neutropenic episodes in 48 leukemic children were reviewed retrospectively from records. Among 136 febrile neutropenic episodes, 68 (50%) episodes were microbiologically documented. Methicillin sensitive coagulase (-) Staphylococcus aureus were the most common isolates from hemoculture (20...
2016: Turkish Journal of Pediatrics
https://www.readbyqxmd.com/read/27922191/full-length-genomic-sequence-analysis-of-new-subtype-3k-hepatitis-e-virus-isolates-with-99-97-nucleotide-identity-obtained-from-two-consecutive-acute-hepatitis-patients-in-a-city-in-northeast-japan
#2
Masahito Miura, Jun Inoue, Mio Tsuruoka, Tsutomu Nishizawa, Shigeo Nagashima, Masaharu Takahashi, Tooru Shimosegawa, Hiroaki Okamoto
Full-length genomic sequences of hepatitis E virus (HEV) obtained from two consecutive cases of acute self-limiting hepatitis E in a city in northeast Japan were determined. Interestingly, two HEV isolates from each patient shared nucleotide identity of 99.97% in 7,225 nucleotides, and a phylogenetic analysis showed that they formed a cluster of Japanese isolates that is considered as a new HEV subtype 3k. The high similarity of HEV sequences of two isolates from these patients in this study suggested that a subtype 3k HEV strain had spread via a commonly distributed food in the city, possibly pig liver...
December 6, 2016: Journal of Medical Virology
https://www.readbyqxmd.com/read/27922116/risk-assessment-of-recent-egyptian-h5n1-influenza-viruses
#3
A-S Arafa, S Yamada, M Imai, T Watanabe, S Yamayoshi, K Iwatsuki-Horimoto, M Kiso, Y Sakai-Tagawa, M Ito, T Imamura, N Nakajima, K Takahashi, D Zhao, K Oishi, A Yasuhara, C A Macken, G Zhong, A P Hanson, S Fan, J Ping, M Hatta, T J S Lopes, Y Suzuki, M El-Husseiny, A Selim, N Hagag, M Soliman, G Neumann, H Hasegawa, Y Kawaoka
Highly pathogenic avian influenza (HPAI) viruses of the H5N1 subtype are enzootic in poultry populations in different parts of the world, and have caused numerous human infections in recent years, particularly in Egypt. However, no sustained human-to-human transmission of these viruses has yet been reported. We tested nine naturally occurring Egyptian H5N1 viruses (isolated in 2014-2015) in ferrets and found that three of them transmitted via respiratory droplets, causing a fatal infection in one of the exposed animals...
December 6, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27922073/a-single-step-method-for-rna-isolation-from-tropical-crops-in-the-field
#4
J-C Breitler, C Campa, F Georget, B Bertrand, H Etienne
The RNAzol RT reagent was used to provide pure RNA from human cells. We develop a protocol using RNAzol RT reagent to extract pure RNA from plants tissues and demonstrate that this RNA extraction method works not only at room temperature but also at elevated temperatures and provides the simplest and most effective single-step method to extract pure and undegraded RNA directly from tropical plants in the field. RNA extraction directly in a complex field environment opens up the way for studying gene-environment interactions at transcriptome level to decipher the complex regulatory network involved in multiple-stress responses...
December 6, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27922055/limited-hiv-1-reactivation-in-resting-cd4-t-cells-from-aviremic-patients-under-protease-inhibitors
#5
Amit Kumar, Wasim Abbas, Sophie Bouchat, Jean-Stéphane Gatot, Sébastien Pasquereau, Kabamba Kabeya, Nathan Clumeck, Stéphane De Wit, Carine Van Lint, Georges Herbein
A latent viral reservoir that resides in resting CD4(+) T cells represents a major barrier for eradication of HIV infection. We test here the impact of HIV protease inhibitor (PI) based combination anti-retroviral therapy (cART) over nonnucleoside reverse transcriptase inhibitor (NNRTI)-based cART on HIV-1 reactivation and integration in resting CD4(+) T cells. This is a prospective cohort study of patients with chronic HIV-1 infection treated with conventional cART with an undetectable viremia. We performed a seven-year study of 47 patients with chronic HIV-infection treated with cART regimens and with undetectable plasma HIV-1 RNA levels for at least 1 year...
December 6, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27921993/can-sergentomyia-diptera-psychodidae-play-a-role-in-the-transmission-of-mammal-infecting-leishmania
#6
Carla Maia, Jérôme Depaquit
Leishmaniases are parasitic diseases caused by protozoa of the genus Leishmania. The parasites, which infect various wild and domestic mammals, including humans, are transmitted by the bite of phlebotomine sand flies belonging to the Phlebotomus genus in the Old World and to several genera (including Lutzomyia, Psychodopygus and Nyssomyia) in the New World. In this paper, we consider the genus Sergentomyia as divided into seven subgenera, mainly based on spermathecal morphology: Sergentomyia, Sintonius, Parrotomyia, Rondanomyia, Capensomyia, Vattieromyia and Trouilletomyia...
2016: Parasite: Journal de la Société Française de Parasitologie
https://www.readbyqxmd.com/read/27921410/downregulation-of-immunosuppressive-molecules-pd-1-and-pd-l1-but-not-pd-l2-in-the-patients-with-multiple-sclerosis
#7
Mohammad Reza Javan, Saeed Aslani, Mohammad Reza Zamani, Javad Rostamnejad, Milad Asadi, Mahdi Farhoodi, Mohammad Hossein Nicknam
Programmed cell death-1 (PD-1) and its ligands, PD-L1 and PD-L2, have been regarded as important immune system regulatory molecules. The aberrant expression of the molecules has been related to several autoimmune disorders. This study is aimed to assess the mRNA expression level of PD-1, PD-L1, and PD-L2 molecules in the peripheral blood mononuclear mells (PBMCs) from multiple sclerosis (MS) patients. PBMCs were isolated from the whole blood of 50 MS and 50 healthy individuals. Total RNA content of the leukocytes was extracted...
August 2016: Iranian Journal of Allergy, Asthma, and Immunology
https://www.readbyqxmd.com/read/27921398/effects-of-rhizobacteria-paenibacillus-polymyxa-apec136-and-bacillus-subtilis-apec170-on-biocontrol-of-postharvest-pathogens-of-apple-fruits
#8
Young Soo Kim, Kotnala Balaraju, Yongho Jeon
In this study, plant growth-promoting rhizobacteria (PGPR) were evaluated as potential biocontrol agents against postharvest pathogens of apple fruits. In vitro bioassays revealed that, out of 30 isolates screened, isolates APEC136 and APEC170 had the most significant inhibitory effects against the mycelial growth of several fungal pathogens. Analysis of 16S ribosomal RNA (rRNA) sequences identified the two effective isolates as Paenibacillus polymyxa and Bacillus subtilis, respectively. The two strains showed greater growth in brain-heart infusion broth than in other growth media...
2016: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/27921393/the-role-of-the-microvascular-network-structure-on-diffusion-and-consumption-of-anti-cancer-drugs
#9
P Mascheroni, R Penta
We investigate the impact of microvascular geometry on the transport of drugs in solid tumors, focusing on the diffusion and consumption phenomena. We embrace recent advances in the asymptotic homogenization literature starting from a double Darcy, double advection-diffusion-reaction system of partial differential equations that is obtained exploiting the sharp length separation between the intercapillary distance and the average tumor size. The geometric information on the microvascular network is encoded into effective hydraulic conductivities and diffusivities, which are numerically computed by solving periodic cell problems on appropriate microscale representative cells...
December 6, 2016: International Journal for Numerical Methods in Biomedical Engineering
https://www.readbyqxmd.com/read/27921358/low-methoxyl-lemon-pectin-attenuates-inflammatory-responses-and-improves-intestinal-barrier-integrity-in-caerulein-induced-experimental-acute-pancreatitis
#10
Yajun Sun, Yue He, Fei Wang, Hao Zhang, Paul de Vos, Jia Sun
SCOPE: Acute pancreatitis (AP) is a common clinical acute abdominal disease. The intestinal injury associated with AP will aggravate the condition retroactively. This study investigates whether the low-methoxyl pectin (LMP) isolated from lemon could attenuate AP and associated intestinal injury. METHODS AND RESULTS: Experimental AP was induced in BALB/c mice by caerulien (CAE) hyperstimulation. Nutritional prophylactic group was pre-fed with 5% LMP supplemented forage three days before AP induction...
December 6, 2016: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/27921344/uhplc-ms-based-hdac-assay-applied-to-bio-guided-microfractionation-of-fungal-extracts
#11
Vincent Zwick, Pierre-Marie Allard, Lucie Ory, Claudia A Simões-Pires, Laurence Marcourt, Katia Gindro, Jean-Luc Wolfender, Muriel Cuendet
INTRODUCTION: Histone deacetylases (HDAC) are considered as promising targets for cancer treatment. Today, four HDAC inhibitors, vorinostat, romidepsin, belinostat, and panobinostat, have been approved by the Food and Drug Administration (FDA) for cancer treatment, while others are in clinical trials. Among them, several are naturally occurring fungal metabolites. OBJECTIVE: To develop and optimise an enzyme assay for bio-guided identification of HDAC inhibitors in fungal strains...
December 5, 2016: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/27921343/complete-genome-sequence-of-streptococcus-troglodytae-tku31-isolated-from-an-oral-cavity-of-chimpanzee-pan-troglodytes
#12
Masaaki Okamoto, Mariko Naito, Mayu Miyanohara, Susumu Imai, Yoshiaki Nomura, Wataru Saito, Yasuko Momoi, Kazuko Takada, Takako Miyabe-Nishiwaki, Masaki Tomonaga, Nobuhiro Hanada
Streptococcus troglodytae TKU31 was isolated from an oral cavity of chimpanzee (Pan troglodytes), and it is the most closely related species to Streptococcus mutans among the mutans group streptococci. The complete sequence of TKU31 genome consisted of a single circular chromosome that was 2,097,874 base pairs in length, with a G + C content of 37.18%. It possessed a total of 2,082 coding sequences (CDSs), 65 tRNAs and five rRNA operons (15 rRNAs). Two clustered regularly interspaced short palindromic repeats (CRISPRs), six insertion sequences (IS) and two predicted prophage elements were identified...
December 6, 2016: Microbiology and Immunology
https://www.readbyqxmd.com/read/27921341/unexpected-effect-of-the-vehicle-grain-ethanol-of-homeopathic-fsh-on-the-in-vitro-survival-and-development-of-isolated-ovine-preantral-follicles
#13
Lartiza F Lima, Rebeca M P Rocha, Ana Beatriz G Duarte, Ivina R Brito, Gerlane M Silva, Giovanna Q Rodrigues, Diana C S Nunes-Pinheiro, Antônia D Sales, Arlindo A Moura, Matthew B Wheeler, Ana Paula R Rodrigues, Cláudio C Campello, José Ricardo Figueiredo
The aims of this study were to investigate the effects of medium replacement system (experiment I) and of FSH presentations (homeopathic - FSH 6cH and allopathic FSH - rFSH; experiment II) on the in vitro development, hormone production and gene expression of isolated ovine preantral follicles cultured for 6 days. In experiment I, secondary follicles were cultured in the α-MEM(+) supplemented with FSH 6cH (0.05 fg/ml) or recombinant bovine FSH (100 ng/ml) without/with daily medium addition. The homeopathic FSH treatments with/without medium addition improved (p < ...
December 5, 2016: Microscopy Research and Technique
https://www.readbyqxmd.com/read/27921337/extended-spectrum-cephalosporin-resistance-among-clinical-isolates-of-enterobacteriaceae-in-west-norway-during-2006-2013-a-prospective-surveillance-study
#14
Haima Mylvaganam, Helge Kolstad, Rebecca Irene Breistein, Grete Lind, Dag Harald Skutlaberg
Routine surveillance of resistance to broad-spectrum cephalosporins in Enterobacteriaceae and phenotypic identification of underlying mechanisms using a simple strategy was commenced in 2006 at our laboratory, serving West Norway. This report focuses on the results until 2013. The classical plasmid-mediated extended spectrum beta-lactamase (ESBLA ) among clinically relevant Escherichia coli isolates showed an increase from 0.6% to 4.3% during the surveillance period, while prevalence for other mechanisms remained stable, below 0...
December 6, 2016: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/27921331/living-in-limbo-latinas-assessment-of-lower-rio-grande-valley-colonias-communities
#15
DeAnne K Hilfinger Messias, Patricia A Sharpe, Lourdes Del Castillo-González, Laura Treviño, Deborah Parra-Medina
Community asset mapping (CAM) is the collective process of identifying local assets and strategizing processes to address public health issues and concerns and improve quality of life. Prior to implementing a community-based physical activity intervention with Latinas in the Texas Lower Rio Grande Valley, promotoras [community health workers] conducted 16 interactive sessions in 8 colonias. The analysis of the transcribed CAM recordings and on-site observational data resulted in the construction of Living in Limbo as the thematic representation of these Latinas' social isolation and marginalization associated with pervasive poverty, undocumented immigration status or lack of citizenship, their fears emanating from threats to physical and emotional safety, and the barriers created by lack of availability and access to resources...
December 5, 2016: Public Health Nursing
https://www.readbyqxmd.com/read/27921303/eosinophils-from-eosinophilic-oesophagitis-patients-have-t-cell-suppressive-capacity-and-express-foxp3
#16
C Lingblom, J Wallander, M Ingelsten, H Bergquist, M Bove, R Saalman, A Welin, C Wennerås
Eosinophilic esophagitis (EoE) is an antigen-driven T cell-mediated chronic inflammatory disease where food and environmental antigens are thought to have a role. Human eosinophils express the immunoregulatory protein galectin-10 and have T cell suppressive capacity similar to regulatory T cells (Tregs ). We hypothesized that one function of eosinophils in EoE might be to regulate the T cell-driven inflammation in the oesophagus. This was tested by evaluating the suppressive capacity of eosinophils isolated from the blood of adult EoE patients in a mixed lymphocyte reaction...
November 5, 2016: Clinical and Experimental Immunology
https://www.readbyqxmd.com/read/27921295/digest-sticklebacks-don-t-just-go-with-the-flow-genetic-differentiation-between-lake-and-stream-populations-due-to-more-than-just-geographic-distance1
#17
Emilie Richards, Aaron Comeault, Jelmer Poelstra
Speciation is thought to occur in large part through the gradual accumulation of genetic differences between partly or completely isolated populations. However, many species differ greatly in their ecology, prompting research into ecology's role in driving genetic differentiation and speciation. Ultimately, understanding diversification requires understanding the factors that allow populations to diverge enough to no longer be considered the same species. This article is protected by copyright. All rights reserved...
December 6, 2016: Evolution; International Journal of Organic Evolution
https://www.readbyqxmd.com/read/27921141/kidney-stones-diseases-and-glycaemic-statuses-focus-on-the-latest-clinical-evidences
#18
REVIEW
Leonardo Spatola, Claudio Angelini, Salvatore Badalamenti, Silvio Maringhini, Giovanni Gambaro
Diabetes and obesity are already recognized as potential risk factors for nephrolithiasis, especially for uric acid stones. Insulin resistance and hyperinsulinemia actively contribute to impaired ability to excrete an acid load and altered ammonium production, leading to a lower urinary pH compared to non-diabetic controls. All these electrolytic disorders play an important role in stone formation and aggregation, especially in uric acid stones. There are still missing points in scientific evidence if the increased risk in stone formation is already existing even in the prediabetic statuses (isolated impaired glucose tolerance, isolated impaired fasting glucose, and associated impaired glucose tolerance/impaired fasting glucose) as well as it is worth to consider the same level of risk...
December 5, 2016: Urolithiasis
https://www.readbyqxmd.com/read/27921108/a-novel-antioxidant-peptide-derived-from-wheat-germ-prevents-high-glucose-induced-oxidative-stress-in-vascular-smooth-muscle-cells-in-vitro
#19
Siyuan Chen, Dingbo Lin, Yulong Gao, Xiaozhou Cao, Xinchun Shen
The ingestion of whole wheat products is responsible for the prevention of a number of chronic diseases. The aim of this study is to characterize the molecular properties of a novel wheat germ-derived antioxidant peptide (AOP) in protection against high glucose-induced oxidative stress in vascular smooth muscle cells (VSMCs). AOP (amino acid sequence AREGETVVPG) was isolated from wheat germ albumin using dual-enzymatic hydrolysis/separation, followed by MS/MS. The in vitro IC50 value for Fe(2+) chelation was 0...
December 6, 2016: Food & Function
https://www.readbyqxmd.com/read/27921034/crowdsourcing-precision-cerebrovascular-health-imaging-and-cloud-seeding-a-million-brains-initiative%C3%A2
#20
David S Liebeskind
Crowdsourcing, an unorthodox approach in medicine, creates an unusual paradigm to study precision cerebrovascular health, eliminating the relative isolation and non-standardized nature of current imaging data infrastructure, while shifting emphasis to the astounding capacity of big data in the cloud. This perspective envisions the use of imaging data of the brain and vessels to orient and seed A Million Brains Initiative™ that may leapfrog incremental advances in stroke and rapidly provide useful data to the sizable population around the globe prone to the devastating effects of stroke and vascular substrates of dementia...
2016: Frontiers in Medicine
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