Read by QxMD icon Read


Y-X Cao, Z-Q Wang, J-X Kang, K Liu, C-F Zhao, Y-X Guo, B-H Jiao
OBJECTIVE: It's of great significance to investigate the novel targets of drugs for the treatment of stroke. In this study, we explored the neuroprotective role of miR-424 in oxygen glucose deprivation (OGD)-induced injuries in PC-12 cells. MATERIALS AND METHODS: PC-12 cells were subjected to OGD stimulation to mimic ischemic injury. The expressions of miR-424 and mitogen-activated protein kinase phosphatase-1 (MKP-1) were altered by transient transfection with miR-424 mimic, miR-424 inhibitor, pEX-MKP-1, or sh-MKP-1...
March 2018: European Review for Medical and Pharmacological Sciences
Z-S Zhang, J Wang, B-Q Zhu, L Ge
OBJECTIVE: To investigate the role of long noncoding RNA (lncRNA) UCA1 in the multiple myeloma (MM) development. PATIENTS AND METHODS: In samples of MM, the expression of UCA1 and TGF-β was investigated using real-time PCR. UCA1 lentiviral small hairpin RNA (shRNA) was transfected in MM cell lines. CCK-8 and colony formation assay were used to detect cell lines proliferation. The cell apoptosis assay was conducted to detect cell apoptosis. Western blot was utilized to detect the protein level of TGF-β...
March 2018: European Review for Medical and Pharmacological Sciences
F-D Sun, P-C Wang, R-L Luan, S-H Zou, X Du
OBJECTIVE: Drug resistance has become an important factor that threatens the survival and prognosis of patients with breast cancer, especially in patients with advanced breast cancer. Several microRNAs have been proved to participate in the resistant process; however, the role of miR-574 in doxorubicin (Dox) resistant breast cancer is still unclear. PATIENTS AND METHODS: Quantitative Real-time poly chain reaction (qRT-PCR) was employed to detect the expression level of miR-574 in breast cancer Dox-resistant MCF-7/Adr cell line and parental MCF-7 cell line...
March 2018: European Review for Medical and Pharmacological Sciences
Z Xing, H-Y Wang, W-Y Su, Y-F Liu, X-X Wang, P Zhan, T-F Lv, Y Song
OBJECTIVE: Aberrant activation of (Wingless and mouse homolog Int-1) Wnt/β-catenin signaling pathways closely involved in the occurrence and progression of several types of human malignancies. This research was undertaken to elucidate the important role of (Wingless and mouse homolog Int-1) in lung cancer. PATIENTS AND METHODS: Wnt3 expression in lung cancers and their respective normal tissues were examined by immunoblotting and immunohistochemistry. Then, Wnt3 was regulated with RNA interference (RNAi) technology in human lung cancer A549 cells, and the cell proliferation, cell cycle, cell invasion/metastasis, and apoptosis were evaluated...
March 2018: European Review for Medical and Pharmacological Sciences
X Yang, W Zhang, S-Q Cheng, R-L Yang
OBJECTIVE: To clarify the role of long non-coding RNA (lncRNA) GACAT3 in invasion and metastasis of non-small cell lung cancer (NSCLC) and its effect on radiotherapy. PATIENTS AND METHODS: The expression of GACAT3 and TIMP2 in cells and tissues of NSCLC were detected by quantitative Real-time PCR (qRT-PCR). The influence of GACAT3 on cell proliferation and the capacity of colony formation were estimated by MTT test and colony forming experiment respectively. Luciferase reporting assay was used to confirm the correlation between GACAT3 and TIMP2...
March 2018: European Review for Medical and Pharmacological Sciences
B-F Yang, W Cai, B Chen
OBJECTIVE: We aimed at detecting the expression of lncRNA SNHG12 in gastric carcinoma and at exploring its effect on the proliferative ability and metastasis of gastric carcinoma. Also, its mechanism was analyzed. PATIENTS AND METHODS: A total of 54 pairs of gastric carcinoma and paracancerous tissues were harvested from patients in our hospital. Clinical data were used for analyzing the relationship between lncRNA SNHG12 and general information of patients with gastric carcinoma...
March 2018: European Review for Medical and Pharmacological Sciences
B Song, H-X Lin, L-L Dong, J-J Ma, Z-G Jiang
OBJECTIVE: Emerging evidence suggests aberrant microRNAs (miRNAs) expression is involved in cancer development through multiple. Although miR338 has shown to have tumor suppression ability and anti-migration effects in some cancers, its regulatory role and molecular mechanism in the development of gastric cancer cells yet remains little known. This work aims to investigate miR-338 in regulating Wnt/β-catenin pathway in epithelial-mesenchymal transition (EMT) in gastric cancers. MATERIALS AND METHODS: Human gastric cancer cells were transfected with either miR-338 mimic or erythropoietin-producing hepatocellular (Eph)A2-targeting siRNA...
March 2018: European Review for Medical and Pharmacological Sciences
W-W Liao, C Zhang, F-R Liu, W-J Wang
OBJECTIVE: MiR-155 has been shown to be up-regulated in hepatocellular carcinoma (HCC) patients. B-cell lymphoma-2 (Bcl-2) interacting mediator of cell death (BIM) regulates cell proliferation and apoptosis, as its down-regulation is involved in HCC onset. Transcriptional factor FoxO3a mediates BIM expression and is related to HCC pathogenesis. Bioinformatics analysis showed targeted regulation of FoxO3a by miR-155. This study aims to investigate whether miR-155 plays a role in mediating FoxO3a/BIM signal pathway and HCC occurrence...
March 2018: European Review for Medical and Pharmacological Sciences
J-X Li, X-M Ding, S Han, K Wang, C-Y Jiao, X-C Li
OBJECTIVE: To investigate the role of mir-637 on the proliferation, migration and apoptosis in human cholangiocarcinoma (CCA) cell line QBC939, and the impact of mir-637 on Cathepsin B (CTSB) expression. MATERIALS AND METHODS: Expression of mir-637 and CTSB in CCA tissues and adjacent non-tumor tissues were measured by Real-time PCR. CTSB expression in human CCA cell lines was detected with or without mir-637 exogenous overexpression through fluorescent quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) method...
March 2018: European Review for Medical and Pharmacological Sciences
S-R Zhang, D-B Li, J-W Xue
OBJECTIVE: Given the important functions of TP53 pathway in various biological processes, this study aimed to investigate the expression of TP53 pathway-related proteins in ovarian carcinoma transplanted subcutaneously in nude mice with and without the presence of p53 inhibitor and to explore possible roles of p53 in the development of ovarian cancer. MATERIALS AND METHODS: Thirty BALB/c-nu female nude mice were randomly divided into model group, control group and p53 inhibitor group (Pftα group)...
March 2018: European Review for Medical and Pharmacological Sciences
X-G Liu, J Xu, F Li, M-J Li, T Hu
OBJECTIVE: Chemoresistance is one of the obstacles for effective treatment of cancers, and recent evidence has shown that microRNAs play critical roles in drug resistance. In this study, we investigated the effect of miR-377 on cisplatin resistance in osteosarcoma (OS). PATIENTS AND METHODS: Tumor tissues from chemoresistant and control OS patients were subjected to Real-time polymerase chain reaction (PCR) to assess miR-377 expression. The effect and mechanism of miR-377 on cisplatin resistance were assessed using Cell Counting Kit (CCK) 8, flow cytometry, Western blot, and luciferase assays in cisplatin-resistant OS cells lines...
March 2018: European Review for Medical and Pharmacological Sciences
D-W Wang, Y-Q Wang, H-S Shu
OBJECTIVE: Extracellular signal-regulated kinase (ERK)/mitogen activated protein kinase (MAPK) signal pathway participates in cell proliferation, cycle, and apoptosis. MiR-16 is down-regulated in the pituitary tumor. This study investigated the role and related mechanism of miR-16 on pituitary tumor proliferation, cycle, and apoptosis. PATIENTS AND METHODS: Dual-luciferase reporter assay was conducted to demonstrate the targeted regulation between miR-16 and MEK1...
March 2018: European Review for Medical and Pharmacological Sciences
Y-M Weng, C-R Ke, J-Z Kong, H Chen, J-J Hong, D-S Zhou
OBJECTIVE: To observe the effects of autophagy-related gene 5 (ATG5) on the proliferation, differentiation, and apoptosis of Mc3T3-E1 osteoblast as well as the effects of ATG5 on apoptosis of osteoblasts under the conditions of non-oxidative stress and oxidative stress. MATERIALS AND METHODS: ATG5 overexpressing and silencing cell lines were established in this experiment with lentiviral vector and transcription activator-like effect or nuclease (Talen) technique, respectively, using Mc3T3-E1 cells...
March 2018: European Review for Medical and Pharmacological Sciences
J-L Li, R Li, Y Gao, W-C Guo, P-X Shi, M Li
OBJECTIVE: Preeclampsia is one of the leading causes of maternal and perinatal deaths. This study mainly explored the mechanism of long non-coding RNA (lncRNA) CCAT1 expression in the placenta of preeclampsia patients and its effect on the progression of preeclampsia. MATERIALS AND METHODS: We used quantitative reverse transcription PCR (qRT‑PCR) to detect the lncRNA CCAT1 expression in 40 preeclampsia and 40 normal pregnancy placenta samples. CCAT1 expression and its relationship with the clinicopathological parameters of preeclampsia was statistically analyzed...
March 2018: European Review for Medical and Pharmacological Sciences
X-H Zhang, H-Y Zhang, S Lu, L-L Jiang, J Wu, Y-L Yang, S-A Zhang
OBJECTIVE: Gestational hypertension is a pregnancy complication that serious damages the maternal and child health. Early onset severe preeclampsia accounts for about 0.9% of the gestational hypertension disease. Conservative treatment is proposed in recent years to early onset severe preeclampsia through delay delivery. Therefore, it is particularly important to explore the pathogenesis of severe preeclampsia. Soluble endoglin (sEng) has been identified as a central factor to induce endothelium dysfunction of preeclampsia, while its specific mechanism is unclear...
March 2018: European Review for Medical and Pharmacological Sciences
C D'Aniello, S Pisconti, S Facchini, C Imbimbo, C Cavaliere
No abstract text is available yet for this article.
March 2018: European Review for Medical and Pharmacological Sciences
Xiaoling Hu, Yuanfang Zhai, Ruyi Shi, Yu Qian, Heyang Cui, Jie Yang, Yanghui Bi, Ting Yan, Jian Yang, Yanchun Ma, Ling Zhang, Yiqian Liu, Guodong Li, Mingsheng Zhang, Yongping Cui, Pengzhou Kong, Xiaolong Cheng
FAT atypical cadherin 1 (FAT1) belongs to the cadherin superfamily and has been reported to regulate cell‑cell adhesion and other cell behaviors, suggesting its pivotal roles in human cancers. We previously identified FAT1 as one of the significant mutant genes in esophageal squamous cell carcinoma (ESCC). In the present study, the knockdown of FAT1 expression in YSE2 and Colo680N cell lines was carried out by lentivirus, and we found that knockdown of FAT1 led to acceleration of cell migration and invasion...
May 2018: Oncology Reports
Yunhu Pan, Hongmei Wang, Debin Ma, Zhiyu Ji, Limin Luo, Fangyu Cao, Fangfang Huang, Yangyang Liu, Yushu Dong, Yitan Chen
The incidence of lung cancer in China increases annually, and effective targets for the diagnosis and treatment of lung cancer are urgently needed. miRNAs are currently considered to be involved in the regulation of tumor development and growth. miR‑24 has been found to contribute to the development of several tumors. Menin is a key tumor suppressor gene, and its expression is generally low in lung cancer. The effects of miR‑24 on the biological behavior of lung cancer cells were detected by MTT and Transwell assays...
May 2018: Oncology Reports
Liankang Sun, Ke Chen, Zhengdong Jiang, Xin Chen, Jiguang Ma, Qingyong Ma, Wanxing Duan
Increasing evidence indicates that pancreatic stellate cells (PSCs) are responsible for the stromal reaction in pancreatic ductal adenocarcinoma (PDAC). The interaction between activated PSCs and PDAC cells and the resultant stromal reaction facilitate cancer progression. Previous findings suggested that cyclooxygenase‑2 (COX‑2) may have a profound role in regulating the proliferation and activation of PSCs in response to pancreatic cancer. Indometacin, a well‑known anti‑inflammatory drug and a non‑selective inhibitor of COX‑2, has been shown to exert anticancer effects in various types of cancer, including PDAC...
May 2018: Oncology Reports
Jing Lin, Jianling Zuo, Yong Cui, Chaoli Song, Xiaojun Wu, Huaizhi Feng, Jian Li, Shuo Li, Qinian Xu, Wenxin Wei, Guanzhong Qiu, Hua He
Acquired chemoresistance refers to tumor cells gradually losing their sensitivity to anticancer drugs during the course of treatment, resulting in tumor progression or recurrence. This phenomenon, which has deleterious outcomes for the patient, has long been observed in patients with glioblastoma receiving temozolomide (TMZ)-based radiochemotherapy. Currently, the mechanisms for acquired TMZ chemoresistance are not fully understood. In the present study, a TMZ-resistant cell line U251R with a 4-fold 50% inhibition concentration compared with its TMZ-sensitive parent cell line was isolated by incremental long-time TMZ treatment in the human glioblastoma cell line U251...
May 2018: Oncology Reports
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"