Read by QxMD icon Read

TPC patch

Armando Carpaneto, Antonella Gradogna
Plant two-pore channels (TPCs) are non-selective cation channels permeable both to monovalent potassium and divalent calcium. We previously developed a technique that allowed the simultaneous determination of the fluxes of these two ions across the channel by a combined use of patch-clamp and fluorescence. In this paper we studied how potassium and calcium fluxes were influenced by modification of cytosolic concentrations of K+ and Ca2+ . A decrease in cytosolic calcium from 2 to 0.5 mM led to a shift of the activation curve of about +60 mV; although at positive potentials currents were very similar, calcium ion permeation was significantly reduced and the ratio between the total and calcium-mediated current increased about two-fold...
May 2018: Biophysical Chemistry
Chien-Chin Yang, Shu-Min Tseng, Carton W Chen
Typical telomeres of linear chromosomes and plasmids of soil bacteria Streptomyces consist of tightly packed palindromic sequences with a terminal protein ('TP') covalently attached to the 5' end of the DNA. Replication of these linear replicons is initiated internally and proceeds bidirectionally toward the telomeres, which leaves single-strand overhangs at the 3' ends. These overhangs are filled by DNA synthesis using the TPs as the primers ('end patching'). The gene encoding for typical TP, tpg, forms an operon with tap, encoding an essential telomere-associated protein, which binds TP and the secondary structures formed by the 3' overhangs...
July 27, 2015: Nucleic Acids Research
Margarida Ruas, Lianne C Davis, Cheng-Chang Chen, Anthony J Morgan, Kai-Ting Chuang, Timothy F Walseth, Christian Grimm, Clive Garnham, Trevor Powell, Nick Platt, Frances M Platt, Martin Biel, Christian Wahl-Schott, John Parrington, Antony Galione
The second messenger NAADP triggers Ca(2+) release from endo-lysosomes. Although two-pore channels (TPCs) have been proposed to be regulated by NAADP, recent studies have challenged this. By generating the first mouse line with demonstrable absence of both Tpcn1 and Tpcn2 expression (Tpcn1/2(-/-)), we show that the loss of endogenous TPCs abolished NAADP-dependent Ca(2+) responses as assessed by single-cell Ca(2+) imaging or patch-clamp of single endo-lysosomes. In contrast, currents stimulated by PI(3,5)P2 were only partially dependent on TPCs...
July 2, 2015: EMBO Journal
Manuel Ruiz-Aravena, Avia Gonzalez-Mendez, Sergio A Estay, Juan D Gaitán-Espitia, Ismael Barria-Oyarzo, José L Bartheld, Leonardo D Bacigalupe
When dispersal is not an option to evade warming temperatures, compensation through behavior, plasticity, or evolutionary adaptation is essential to prevent extinction. In this work, we evaluated whether there is physiological plasticity in the thermal performance curve (TPC) of maximum jumping speed in individuals acclimated to current and projected temperatures and whether there is an opportunity for behavioral thermoregulation in the desert landscape where inhabits the northernmost population of the endemic frog Pleurodema thaul...
December 2014: Ecology and Evolution
Po-Chih Kuo, Yong-Sheng Chen, Li-Fen Chen, Jen-Chuen Hsieh
Visual decoding and encoding are crucial aspects in investigating the representation of visual information in the human brain. This paper proposes a bidirectional model for decoding and encoding of visual stimulus based on manifold representation of the temporal and spatial information extracted from magnetoencephalographic data. In the proposed decoding process, principal component analysis is applied to extract temporal principal components (TPCs) from the visual cortical activity estimated by a beamforming method...
November 15, 2014: NeuroImage
Anna Boccaccio, Joachim Scholz-Starke, Shin Hamamoto, Nina Larisch, Margherita Festa, Paul Vijay Kanth Gutla, Alex Costa, Petra Dietrich, Nobuyuki Uozumi, Armando Carpaneto
Two-pore channel proteins (TPC) encode intracellular ion channels in both animals and plants. In mammalian cells, the two isoforms (TPC1 and TPC2) localize to the endo-lysosomal compartment, whereas the plant TPC1 protein is targeted to the membrane surrounding the large lytic vacuole. Although it is well established that plant TPC1 channels activate in a voltage- and calcium-dependent manner in vitro, there is still debate on their activation under physiological conditions. Likewise, the mode of animal TPC activation is heavily disputed between two camps favoring as activator either nicotinic acid adenine dinucleotide phosphate (NAADP) or the phosphoinositide PI(3,5)P₂...
November 2014: Cellular and Molecular Life Sciences: CMLS
Shirley L Markant, Lourdes Adriana Esparza, Jesse Sun, Kelly L Barton, Lisa M McCoig, Gerald A Grant, John R Crawford, Michael L Levy, Paul A Northcott, David Shih, Marc Remke, Michael D Taylor, Robert J Wechsler-Reya
Medulloblastoma is the most common malignant brain tumor in children. Although aggressive surgery, radiation, and chemotherapy have improved outcomes, survivors suffer severe long-term side effects, and many patients still succumb to their disease. For patients whose tumors are driven by mutations in the sonic hedgehog (SHH) pathway, SHH antagonists offer some hope. However, many SHH-associated medulloblastomas do not respond to these drugs, and those that do may develop resistance. Therefore, more effective treatment strategies are needed for both SHH and non-SHH-associated medulloblastoma...
October 15, 2013: Cancer Research
Paul Vijay Kanth Gutla, Anna Boccaccio, Alexis De Angeli, Franco Gambale, Armando Carpaneto
Polyunsaturated fatty acids (PUFAs) are powerful modulators of several animal ion channels. It is shown here that PUFAs strongly affect the activity of the Slow Vacuolar (SV) channel encoded by the plant TPC1 gene. The patch-clamp technique was applied to isolated vacuoles from carrot taproots and Arabidopsis thaliana mesophyll cells and arachidonic acid (AA) was chosen as a model molecule for PUFAs. Our study was extended to different PUFAs including the endogenous alpha-linolenic acid (ALA). The addition of micromolar concentrations of AA reversibly inhibited the SV channel decreasing the maximum open probability and shifting the half activation voltage to positive values...
October 2012: Journal of Experimental Botany
Hsiu-Hui Tsai, Chih-Hung Huang, Ingrid Tessmer, Dorothy A Erie, Carton W Chen
Linear chromosomes and linear plasmids of Streptomyces possess covalently bound terminal proteins (TPs) at the 5' ends of their telomeres. These TPs are proposed to act as primers for DNA synthesis that patches the single-stranded gaps at the 3' ends during replication. Most ('archetypal') Streptomyces TPs (designated Tpg) are highly conserved in size and sequence. In addition, there are a number of atypical TPs with heterologous sequences and sizes, one of which is Tpc that caps SCP1 plasmid of Streptomyces coelicolor...
March 2011: Nucleic Acids Research
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"