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S H Kim, J G Kang, C S Kim, S-H Ihm, M G Choi, H J Yoo, S J Lee
PURPOSE: The influence of the dipeptidyl peptidase-IV inhibitor gemigliptin alone or in combination with the histone deacetylase inhibitor PXD101 on survival of thyroid carcinoma cells was investigated. METHODS: SW1736, TPC-1, 8505C and BCPAP human thyroid carcinoma cells were used. To assess cell survival, cell viability, the percentage of viable cells and dead cells, cytotoxic activity, ATP levels and FACS analysis were measured. To validate the impact of gemigliptin combined with PXD101, the interactions were estimated by obtaining combination index in cells treated with two agents...
November 16, 2017: Journal of Endocrinological Investigation
Rajalekshmi Maheshwari, Chandrashekara Shastry Shreedhara, Picheswara Rao Polu, Renuka Suresh Managuli, Seena Kanniparambil Xavier, Richard Lobo, Manjunath Setty, Srinivas Mutalik
Background: Sansevieria roxburghiana Schult. and Schult. f. (Asparagaceae) grows in India, Indonesia, Sri Lanka, and tropical Africa. Even though the plant has been traditionally used for the treatment of many ailments, the antioxidant and antiproliferative activities of S. roxburghiana methanol extract and its fractions have not yet been explored. Materials and Methods: Quantitative estimation of phenols and different antioxidant assays were performed using standard methods...
October 2017: Pharmacognosy Magazine
Amit Kaushik, Athar Husain, Harshika Awasthi, Dewasya Pratap Singh, Raziuddin Khan, Dayanandan Mani
Background: In Ayurveda, five basic extraction procedures are mentioned in order of their decreasing potency. Swaras is considered as the most potent followed by, kalka, kwatha, fanta and hima. Objective: Present study was carried out to investigate the antioxidant and hepatoprotective potential of swaras and hima extracts of T.cordifolia and B. diffusa. Materials and Methods: Swaras and hima extracts of T. cordifolia and B. diffusa were prepared...
October 2017: Pharmacognosy Magazine
Sivapragasam Gothai, Katyakyini Muniandy, Mazni Abu Zarin, Tan Woan Sean, S Suresh Kumar, Murugan A Munusamy, Sharida Fakurazi, Palanisamy Arulselvan
Background: Moringa oleifera (MO), commonly known as the drumstick tree, is used in folklore medicine for the treatment of skin disease. Objective: The objective of this study is to evaluate the ethyl acetate (EtOAc) fraction of MO leaves for in vitro antibacterial, antioxidant, and wound healing activities and conduct gas chromatography-mass spectrometry (GC-MS) analysis. Materials and Methods: Antibacterial activity was evaluated against six Gram-positive bacteria and 10 Gram-negative bacteria by disc diffusion method...
October 2017: Pharmacognosy Magazine
Jing Zheng, Huiling Li, Dongting Xu, Huijuan Zhu
Tyrosine kinase FYN-a member of the SRC family of kinases-is associated with mediating mitogenic signals and regulating cell cycle entry, growth, and proliferation. It was hypothesized that FYN is upregulated in thyroid carcinoma, which plays a critical role in tumorigenesis. FYN expression level in thyroid carcinoma tissue and tumor cell lines was determined. Also, the effects of FYN on thyroid cancer (TC) growth, signaling, cell cycle, and apoptosis were evaluated in vitro. FYN was knocked down in thyroid cancer cells (TPC-1) by siRNA to investigate the effects on cell proliferation, invasion, and migration...
November 2017: Cancer Biotherapy & Radiopharmaceuticals
Gaoyang Deng, Zhonggang Wang
Triptycene-based cyanate monomers 2,6,14-tricyanatotriptycene (TPC) and 2,6,14-tris(4-cyanatophenyl)triptycene (TPPC) that contain different numbers of benzene rings per molecule were synthesized, from which two microporous cyanate resins PCN-TPC and PCN-TPPC were prepared. Of interest is the observation that the two polymers have very similar porosity parameters, but PCN-TPPC uptakes considerably higher benzene (77.8 wt %) than PCN-TPC (17.6 wt %) at room temperature since the higher concentration of phenyl groups in PCN-TPPC enhances the π-π interaction with benzene molecules...
November 15, 2017: ACS Applied Materials & Interfaces
Asumi Iesato, Teruo Nakamura, Hiroto Izumi, Takeshi Uehara, Ken-Ichi Ito
This study was designed to examine the involvement of PATZ1 in carcinogenesis and dedifferentiation of thyroid cancer. Immunohistochemistry on clinical specimens indicated nuclear PATZ1 expression in all normal thyroid glands and adenomatous goiter, while nuclear PATZ1 expression decreased along with the dedifferentiation of thyroid cancer. Knockdown of nuclear PATZ1 by siRNA in an immortalized normal follicular epithelial cell line (Nthy-ori 3-1) altered cellular morphology and significantly increased cell proliferation, migration, and invasion...
October 10, 2017: Oncotarget
Sandip Patel, Dev Churamani, Eugen Brailoiu
Two-pore channels (TPCs) are two-domain members of the voltage-gated ion channel superfamily that localize to acidic organelles. Their mechanism of activation (ligands such as NAADP/PI(3,5)P2 versus voltage) and ion selectivity (Ca(2+) versus Na(+)) is debated. Here we report that a cluster of arginine residues in the first domain required for selective voltage-gating of TPC1 map not to the voltage-sensing fourth transmembrane region (S4) but to a cytosolic downstream region (S4-S5 linker). These residues are conserved between TPC isoforms suggesting a generic role in TPC activation...
December 2017: Cell Calcium
Siti Zawanah Halim, Zainul Amiruddin Zakaria, Maizatul Hasyima Omar, Norhafizah Mohtarrudin, Ikarastika Rahayu Abdul Wahab, Muhammad Nazrul Hakim Abdullah
BACKGROUND: Melastoma malabathricum L. (family Melastomaceae; MM) and Muntingia calabura L. (family Elaeocarpaceae; MC) have been separately reported to possess gastroprotective activity. In an attempt to develop a pharmaceutical product with antiulcer potential, the synergistic gastroprotective activity of methanolic extract of a mixture of MM and MC (MMMC) at various ratios was evaluated in rat models. METHODS: Rats were pre-treated orally with 2% Tween 80 (vehicle), 100 mg/kg ranitidine (reference drug) or MMMC (ratios of 1:1, 1:3 and 3:1 (v/v); doses of 15, 150 or 300 mg/kg) and then subjected to the ethanol-induced gastric ulcer or pyloric ligation assays...
November 9, 2017: BMC Complementary and Alternative Medicine
Federica Giusti, Giovanni Caprioli, Massimo Ricciutelli, Elisabetta Torregiani, Sauro Vittori, Gianni Sagratini
Consumption of legumes has become popular among health-conscious consumers due to the high levels of nutrients such as proteins, dietary fibres, minerals, vitamins and micronutrients like polyphenols. Ten legumes cultivars organically and conventionally grown were compared regarding their chemical profiles in terms of total phenolic content (TPC), total anthocyanin content (TAC), total flavonoid content (TFC) and their antioxidant capacity. The chemical profiles (i.e. free phenolics and anthocyanins) were determined with aid of new high-performance liquid chromatography-diode array detection (HPLC-DAD) method able to identify two individual anthocyanins and 15 polyphenols...
November 8, 2017: International Journal of Food Sciences and Nutrition
Dehua Zhou, Zhou Li, Xuefeng Bai
BACKGROUND Papillary thyroid carcinoma (PTC) is associated with mutations of BRAFV600E and RET/PTC and high levels of expression of nuclear factor-κB (NF-κB). However, few studies have focused on the association between NF-κB expression and mutations in BRAFV600E and RET/PTC, especially regarding PTC cell proliferation and migration. The aim of this in vitro study was to investigate the effect of BRAFV600E or RET/PTC on NF-κB expression, cell proliferation and cell migration in four established PTC cell lines...
November 8, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
Ginés B Martínez-Hernández, Noelia Castillejo, María Del M Carrión-Monteagudo, Francisco Artés, Francisco Artés-Hernández
The main nutritional/bioactive compounds (protein; aminoacids, AA; fucose; minerals; vitamins B12 and C; and total phenolic content, TPC) of nine commercial algae powders, used as food supplements, were studied. Undaria pinnatifida showed the highest protein/aminoacid contents (51.6/54.4 g 100 g(-1)). Among brown macroalgae, Himanthalia elongata showed the highest fucose content (26.3 g kg(-1)) followed by Laminaria ochroleuca (22.5 g kg(-1)). Mineral contents of 15-24% were observed in the algae, being particularly excellent sources of iodine (69...
January 1, 2017: Food Science and Technology International, Ciencia y Tecnología de Los Alimentos Internacional
Diana Dzidziguri, Irina Modebadze, Ekaterine Bakuradze, Giorgi Mosidze, Manana Berulava
OBJECTIVES: Cell proliferation is known to be controlled by many networks of regulatory proteins. These multiple and complicated mechanisms of control are still being investigated. The aim of the present study is to determine the different properties of adult rat brain thermostable protein complex (TPC) which affect cell proliferation. MATERIALS AND METHODS: This experimental study used brain, kidney and liver tissue from adult (150-170 g) and adolescent (7, 10, 21, 28 days) white rats, adult pigeons and mice...
January 2018: Cell Journal
Weimin Xu, Hairong Ye, Yilian Zhu, Wenjun Ding, Jihong Fu, Long Cui, Peng Du
BACKGROUND: Ileal pouch-anal anastomosis (IPAA) is recommended for patients with ulcerative colitis (UC) in terms of surgical treatment. Measuring surgical complications of IPAA and long-term quality of life (QOL) are important to achieve an acceptable risk/benefit ratio for patients with UC. MATERIALS AND METHODS: Patients with UC who underwent total proctocolectomy (TPC) with IPAA from February 2008 to July 2016 at our institute were included. Early surgical complications were defined as mechanical/infectious events within one month after IPAA...
November 7, 2017: International Journal of Surgery
Chu-Sheng Huang, Si-Yuan Ma, Hong-Xing Liu, Qian Lu, Ling-Gao Shi, Na Liao, Liu-Bin Wei
To investigate the chemical compounds from the roots of Actinidia rufa, nine compounds were isolated by various column chromatography on silica gel and Sephadex LH-20, and high performance liquid chromatography (HPLC). Their structures were elucidated as 2α, 3β, 19α, 23, 24-pentahydroxyurs-12-en-28-oic acid-28-O-β-D-glucopyranoside (1), 2α, 3α, 19α, 24-tetrahydroxyurs-12-en-28-oic acid-28-O-β-D-glucopyranoside (2), 2α, 3α, 24-trihydroxyurs-12-en-28-oic acid (3), 2α, 3α, 24-trihydroxyolean-12-en-28-oic acid (4), 2α, 3α, 23, 24-tetrahydroxyurs -12-en-28-oic acid (5), 2α, 3β, 23, 24-tetrah-ydroxyurs-12-en-28-oic acid (6), 2α, 3β, 23-trihydroxy-12-en-28-oic acid (7), 2α, 3β, 23-trihydroxyurs-12, 20(30)-dien-28-oic acid (8), and 2α, 3α, 23-trihydroxyurs-12, 20(30)-dien-28-oic acid (9)...
July 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
Claudia M Asensio, Alejandro J Paredes, Maria P Martin, Daniel A Allemandi, Valeria Nepote, Nelson R Grosso
Release kinetics of the volatile compounds of oregano EO microcapsules and the relation with the antioxidant activity were studied. Different wall material (WM) to core (C) ratios (1:1 and 2:1; WM:C), addition of colloidal silicon dioxide (CSD); and different storage conditions: 23 °C (room temperature; R) and 4 °C (fridge temperature; F) were evaluated for 90 d. Volatile compounds, total phenolic content (TPC), free radical scavenging activity (FRSA), and Trolox equivalent antioxidant capacity (TEAC) were measured...
November 2, 2017: Journal of Food Science
Nurul Syahirah Shamsol Anuar, Aeyshah Abang Kassim, Motoo Utsumi, Koji Iwamoto, Masafumi Goto, Kazuya Shimizu, Nor'azizi Othman, Zuriati Zakaria, Norio Sugiura, Hirofumi Hara
Geosmin and 2-methylisoborneol (MIB) outbreaks in tropical water bodies, such as Southeast Asia, by actinomycetes have not yet been elucidated in detail. Six Streptomyces isolates from lowland environments in Malaysia were selected and evaluated for their odor production under different temperatures. The gene responsible for the production of geosmin, geoA, was detected in all isolates, while only two isolates harbored tpc, which is responsible for 2-MIB production. This result suggested that geosmin and 2-MIB synthesis pathway genes already existed in the environment in the Tropics of Southeast Asia...
October 31, 2017: Microbes and Environments
Chikkavadaragudi Ramachandra Shobha, Vishwanath Prashant, Prashant Akila, Rangaswamy Chandini, Maduvanahalli Nataraj Suma, Hattur Basavanagowdappa
BACKGROUND: Natural products have gained importance recently for the treatment of obesity and its complications, partly because of the side effects of modern drugs.Hence, we aimed to study and compare the effect of varying concentrations of Momordicacharantiaon adipogenesis and adipolysis using 3T3-L1 pre-adipocyte cell lines. METHODS: 3T3-L1 pre-adipocytes were procured from the National Center for Cell Sciences, Pune, and cultured in Dulbecco's Modified Eagle's Media (DMEM) supplemented with 20% fetal bovine serum (FBS) and 1 mM L-glutamine...
October 2017: Reports of Biochemistry & Molecular Biology
Kuan-Hung Lin, Ming-Chih Shih, Po Wang, Yi-Ping Yu, Chun-Ping Lu
The objective of this study was to evaluate the antioxidant activities of Mesona procumbens ethanolic extracts (MPEEs) which displayed variable antioxidant levels with different ethanolic concentrations. Compared to MPEEs with 20, 40 and 80% ethanol, the 60% MPEE exhibited a higher total phenol content (TPC), total flavonoid content (TFC), 1,1-diphenyl-2-picryl hydrazyl (DPPH)- and 2,2-azino-bis-(3-ethyl benzothiazoline-6-sulphonic acid (ABTS)-scavenging activities, reducing power, protection of Raw 264.7 mouse macrophages against H2O2-induced damage, and inhibition of reactive oxygen species (ROS) induced by 2,2-azobis(2-amidino propane) dihidrochloride (AAPH)...
October 25, 2017: Natural Product Research
Donia Anvari, Rashid Jamei
BACKGROUND: Medicinal plants contain large amount of natural antioxidant compounds, which enable them to neutralize free radicals. Asteraceae family is important source of phytochemical compounds. OBJECTIVE: The objective of this research is to find out the antioxidant potential and radical scavenging from leaf and flowers of five species (Artemisia absinthium L., Arctium lappa L., Silybum marianum L., Centaurea cyanus L. and Echinops ritro L.) of the Asteraceae family...
October 23, 2017: Recent Patents on Food, Nutrition & Agriculture
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