Satish Kumar, Tusar K Acharya, Shamit Kumar, Tejas P Rokade, Nilesh K Das, Saurabh Chawla, Luna Goswami, Chandan Goswami
Treating different types of bone defects is difficult, complicated, time-consuming, and expensive. Here, we demonstrate that transient receptor potential cation channel subfamily V member 4 (TRPV4), a mechanosensitive, thermogated, and nonselective cation channel, is endogenously present in the mesenchymal stem cells (MSCs). TRPV4 regulates both cytosolic Ca2+ levels and mitochondrial health. Accordingly, the hydrogel made from a natural modified biopolymer carboxymethyl tamarind CMT-Hy and encapsulated with TRPV4-modulatory agents affects different parameters of MSCs, such as cell morphology, focal adhesion points, intracellular Ca2+ , and reactive oxygen species- and NO-levels...
March 12, 2024: ACS Biomaterials Science & Engineering