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Stem cell,hydrogel,scaffold

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https://www.readbyqxmd.com/read/28088670/fibrin-hydrogels-induce-mixed-dorsal-ventral-spinal-neuron-identities-during-differentiation-of-human-induced-pluripotent-stem-cells
#1
John M Edgar, Meghan Robinson, Stephanie M Willerth
: We hypothesized that generating spinal motor neurons (sMNs) from human induced pluripotent stem cell (hiPSC)-derived neural aggregates (NAs) using a chemically-defined differentiation protocol would be more effective inside of 3D fibrin hydrogels compared to 2D poly-L-ornithine(PLO)/laminin-coated tissue culture plastic surfaces. We performed targeted RNA-Seq using next generation sequencing to determine the substrate-specific differences in gene expression that regulate cell phenotype...
January 11, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28079980/induction-of-mesenchymal-stem-cell-differentiation-in-the-absence-of-soluble-inducer-for-cutaneous-wound-regeneration-by-a-chitin-nanofibers-based-hydrogel
#2
Kangquan Shou, Yao Huang, Baiwen Qi, Xiang Hu, Zhanjun Ma, Ang Lu, Chao Jian, Lina Zhang, Aixi Yu
Transplantation of bone marrow mesenchymal stem cells (BMSCs) has been considered as a promising strategy for wound healing. However, poor viability of engrafted BMSCs and limited capabilities of differentiation into the desired cell types in wounds often hinder their application. Few studies report the induction of BMSCs differentiation into the skin regeneration related cell types using natural biopolymer, e.g., chitin and its derivative. Here we utilized a chitin nanofibers(CNFs) hydrogel as a directive cue to induce BMSCs differentiation for enhancing cutaneous wound regeneration in the absence of cell-differentiating factors...
January 12, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28070774/restoring-fertility-with-cryopreserved-prepubertal-testicular-tissue-perspectives-with-hydrogel-encapsulation-nanotechnology-and-bioengineered-scaffolds
#3
Maxime Vermeulen, Jonathan Poels, Francesca de Michele, Anne des Rieux, Christine Wyns
New and improved oncological therapies are now able to cure more than 80% of cancer-affected children in Europe. However, such treatments are gonadotoxic and result in fertility issues, especially in boys who are not able to provide a sperm sample before starting chemo/radiotherapy because of their prepubertal state. For these boys, cryopreservation of immature testicular tissue (ITT) is the only available option, aiming to preserve spermatogonial stem cells (SSCs). Both slow-freezing and vitrification have been investigated to this end and are now applied in a clinical setting for SSC cryopreservation...
January 9, 2017: Annals of Biomedical Engineering
https://www.readbyqxmd.com/read/28058831/peptide-tethered-hydrogel-scaffold-promotes-recovery-from-spinal-cord-transection-via-synergism-with-mesenchymal-stem-cells
#4
Li-Ming Li, Min Han, Xinchi Jiang, Xianzhen Yin, Fu Chen, Tian-Yuan Zhang, Hao Ren, Jiwen Zhang, Zhong Chen, Tingjun Hou, HongWei Ouyang, Yasuhiko Tabata, Youqing Shen, Jian-Qing Gao
Spinal cord injury (SCI) is one of the most devastating injuries. Treatment strategies for SCI are required to overcome comprehensive issues. Implantation of biomaterial scaffolds and stem cells has been demonstrated to be promising strategies. However, a comprehensive recovery effect is difficult to achieve. In the comprehensive treatment process, the specific roles of the implanted scaffolds and of stem cells in combined strategy are usually neglected. In this study, a peptide-modified scaffold is developed based on hyaluronic acid and an adhesive peptide PPFLMLLKGSTR...
January 6, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28057917/an-integrated-miniature-bioprocessing-for-personalized-human-induced-pluripotent-stem-cell-expansion-and-differentiation-into-neural-stem-cells
#5
Haishuang Lin, Qiang Li, Yuguo Lei
Human induced pluripotent stem cells (iPSCs) are ideal cell sources for personalized cell therapies since they can be expanded to generate large numbers of cells and differentiated into presumably all the cell types of the human body in vitro. In addition, patient specific iPSC-derived cells induce minimal or no immune response in vivo. However, with current cell culture technologies and bioprocessing, the cost for biomanufacturing clinical-grade patient specific iPSCs and their derivatives are very high and not affordable for majority of patients...
January 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28041686/evaluation-of-a-commercially-available-hyaluronic-acid-hydrogel-restylane-as-injectable-scaffold-for-dental-pulp-regeneration-an-in%C3%A2-vitro-evaluation
#6
Vanessa Chrepa, Obadah Austah, Anibal Diogenes
INTRODUCTION: Regenerative endodontic procedures (REPs) are viable alternatives for treating immature teeth, yet these procedures do not predictably lead to pulp-dentin regeneration. A true bioengineering approach for dental pulp regeneration requires the incorporation of a scaffold conducive with the regeneration of the pulp-dentin complex. Several materials have been proposed as scaffolds for REPs; nonetheless, the majority are not eligible for immediate clinical chairside use. Thus, the aim of this study was to evaluate Restylane, a Food and Drug Administration-approved hyaluronic acid-based gel, as possible scaffold for REPs...
December 29, 2016: Journal of Endodontics
https://www.readbyqxmd.com/read/28038681/nanostructured-scaffold-as-a-determinant-of-stem-cell-fate
#7
REVIEW
Lekshmi Krishna, Kamesh Dhamodaran, Chaitra Jayadev, Kaushik Chatterjee, Rohit Shetty, S S Khora, Debashish Das
The functionality of stem cells is tightly regulated by cues from the niche, comprising both intrinsic and extrinsic cell signals. Besides chemical and growth factors, biophysical signals are important components of extrinsic signals that dictate the stem cell properties. The materials used in the fabrication of scaffolds provide the chemical cues whereas the shape of the scaffolds provides the biophysical cues. The effect of the chemical composition of the scaffolds on stem cell fate is well researched. Biophysical signals such as nanotopography, mechanical forces, stiffness of the matrix, and roughness of the biomaterial influence the fate of stem cells...
December 30, 2016: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28024612/fabrication-of-a-three-dimensional-%C3%AE-tricalcium-phosphate-gelatin-containing-chitosan-based-nanoparticles-for-sustained-release-of-bone-morphogenetic-protein-2-implication-for-bone-tissue-engineering
#8
Farshid Bastami, Zahrasadat Paknejad, Maissa Jafari, Majid Salehi, Maryam Rezai Rad, Arash Khojasteh
Fabrication of an ideal scaffold having proper composition, physical structure and able to have sustained release of growth factors still is challenging for bone tissue engineering. Current study aimed to design an appropriate three-dimensional (3-D) scaffold with suitable physical characteristics, including proper compressive strength, degradation rate, porosity, and able to sustained release of bone morphogenetic protein-2 (BMP2), for bone tissue engineering. A highly porous 3-D β-tricalcium phosphate (β-TCP) scaffolds, inside of which two perpendicular canals were created, was fabricated using foam-casting technique...
March 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/27998214/fabrication-of-a-highly-aligned-neural-scaffold-via-a-table-top-stereolithography-3d-printing-and-electrospinning
#9
Se-Jun Lee, Margaret Nowicki, Brent Harris, Lijie Grace Zhang
Three-dimensional (3D) bioprinting is a rapidly emerging technique in the field of tissue engineering to fabricate extremely intricate and complex biomimetic scaffolds in the range of micrometers. Such customized 3D printed constructs can be used for the regeneration of complex tissues such as cartilage, vessels, and nerves. However, the 3D printing techniques often offer limited control over the resolution and compromised mechanical properties due to short selection of printable inks. To address these limitations, we combined stereolithography and electrospinning techniques to fabricate a novel 3D biomimetic neural scaffold with a tunable porous structure and embedded aligned fibers...
January 11, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/27995768/substrate-stress-relaxation-regulates-scaffold-remodeling-and-bone-formation-in-vivo
#10
Max Darnell, Simon Young, Luo Gu, Nisarg Shah, Evi Lippens, James Weaver, Georg Duda, David Mooney
The rate of stress relaxation of adhesion substrates potently regulates cell fate and function in vitro, and in this study the authors test whether it can regulate bone formation in vivo by implanting alginate gels with differing rates of stress-relaxation carrying human mesenchymal stem cells into rat calvarial defects. After three months, the rats that received fast-relaxing hydrogels (t1/2 ≈ 50 s) show significantly more new bone growth than those that received slow-relaxing, stiffness-matched hydrogels...
December 20, 2016: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/27984148/evaluation-of-alginate-hydrogel-cytotoxicity-on-three-dimensional-culture-of-type-a-spermatogonial-stem-cells
#11
Maryam Jalayeri, Afshin Pirnia, Elaheh Poorazizi Najafabad, Ali Mohammad Varzi, Mohammadreza Gholami
The culture of spermatogonial cells for future transplantation, based on the specific biology of these cells is important and necessary. Recently, the use of scaffolds especially alginate for culturing stem cells has been the focus of many researchers. The aim of this study was to evaluate the cytotoxicity of alginate hydrogels to cultures of type A spermatogonial stem cells. Spermatogonial stem cells of 6day-old immature mice were isolated by surgery; thereafter, the cells were purified by MACS using antibodies against thy-1 and C-kit and cultured on a layer of laminin...
February 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27975097/an-injectable-collagen-poly-%C3%AE-glutamic-acid-hydrogel-as-a-scaffold-of-stem-cells-and-%C3%AE-lipoic-acid-for-enhanced-protection-against-renal-dysfunction
#12
Sun-Hee Cho, Jung-Ran Noh, Mi Young Cho, Min-Jeong Go, Yong-Hoon Kim, Eun Sung Kang, Yong Ho Kim, Chul-Ho Lee, Yong Taik Lim
Mesenchymal stem cells (MSCs) can ameliorate renal injury and accelerate repair of acute kidney injury. Herein, we developed a collagen/poly(γ-glutamic acid) (γ-PGA) hydrogel as an injectable scaffold for the delivery of mouse MSCs (mMSCs) and anti-oxidant drugs into injured sites. By the introduction of γ-PGA into conventional collagen, the viscosity of collagen was reduced at ambient temperature for easy handling, while the elastic and viscous moduli of collagen were increased and a new porous structure was generated near body temperature...
December 15, 2016: Biomaterials Science
https://www.readbyqxmd.com/read/27966916/injectable-and-3d-bioprinted-polysaccharide-hydrogels-from-cartilage-to-osteochondral-tissue-engineering
#13
Janani Radhakrishnan, Anuradha Subramanian, Uma Maheswari Krishnan, Swaminathan Sethuraman
Biomechanical performance of functional cartilage is executed by the exclusive anisotropic composition and spatially varying intricate architecture in articulating ends of diarthrodial joint. Osteochondral tissue constituting the articulating ends comprise superfical soft cartilage over hard subchondral bone sandwiching interfacial soft-hard tissue. The shock-absorbent, lubricating property of cartilage and mechanical stability of subchondral bone regions are rendered by extended chemical structure of glycosaminoglycans and mineral deposition, respectively...
December 14, 2016: Biomacromolecules
https://www.readbyqxmd.com/read/27956366/biohybrid-cardiac-ecm-based-hydrogels-improve-long-term-cardiac-function-post-myocardial-infarction
#14
Yael Efraim, Hadar Sarig, Noa Cohen Anavy, Udi Sarig, Elio de Berardinis, Su-Yin Chaw, Muthukumar Krishnamoorthi, Jérôme Kalifa, Hanumakumar Bogireddi, Thang Vu Duc, Theodoros Kofidis, Limor Baruch, Freddy Y C Boey, Subbu S Venkatraman, Marcelle Machluf
: Injectable scaffolds for cardiac tissue regeneration are a promising therapeutic approach for progressive heart failure following myocardial infarction (MI). Their major advantage lies in their delivery modality that is considered minimally invasive due to their direct injection into the myocardium. Biomaterials comprising such scaffolds should mimic the cardiac tissue in terms of composition, structure, mechanical support, and most importantly, bioactivity. Nonetheless, natural biomaterial-based gels may suffer from limited mechanical strength, which often fail to provide the long-term support required by the heart for contraction and relaxation...
December 9, 2016: Acta Biomaterialia
https://www.readbyqxmd.com/read/27922061/thermo-sensitive-hydrogels-combined-with-decellularised-matrix-deliver-bfgf-for-the-functional-recovery-of-rats-after-a-spinal-cord-injury
#15
He-Lin Xu, Fu-Rong Tian, Cui-Tao Lu, Jie Xu, Zi-Liang Fan, Jing-Jing Yang, Pian-Pian Chen, Ya-Dong Huang, Jian Xiao, Ying-Zheng Zhao
Because of the short half-life, either systemic or local administration of bFGF shows significant drawbacks to spinal injury. In this study, an acellular spinal cord scaffold (ASC) was encapsulated in a thermo-sensitive hydrogel to overcome these limitations. The ASC was firstly prepared from the spinal cord of healthy rats and characterized by scanning electronic microscopy and immunohistochemical staining. bFGF could specifically complex with the ASC scaffold via electrostatic or receptor-mediated interactions...
December 6, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27883051/lipid-coated-microbubbles-and-low-intensity-pulsed-ultrasound-enhance-chondrogenesis-of-human-mesenchymal-stem-cells-in-3d-printed-scaffolds
#16
Mitra Aliabouzar, Lijie Grace Zhang, Kausik Sarkar
Lipid-coated microbubbles are used to enhance ultrasound imaging and drug delivery. Here we apply these microbubbles along with low intensity pulsed ultrasound (LIPUS) for the first time to enhance proliferation and chondrogenic differentiation of human mesenchymal stem cells (hMSCs) in a 3D printed poly-(ethylene glycol)-diacrylate (PEG-DA) hydrogel scaffold. The hMSC proliferation increased up to 40% after 5 days of culture in the presence of 0.5% (v/v) microbubbles and LIPUS in contrast to 18% with LIPUS alone...
November 24, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27865962/pharmacologically-active-microcarriers-delivering-bdnf-within-a-hydrogel-novel-strategy-for-human-bone-marrow-derived-stem-cells-neural-neuronal-differentiation-guidance-and-therapeutic-secretome-enhancement
#17
Saikrishna Kandalam, Laurence Sindji, Gaëtan J-R Delcroix, Fabien Violet, Xavier Garric, Emilie M André, Paul C Schiller, Marie-Claire Venier-Julienne, Anne des Rieux, Jérôme Guicheux, Claudia N Montero-Menei
: Stem cells combined with biodegradable injectable scaffolds releasing growth factors hold great promises in regenerative medicine, particularly in the treatment of neurological disorders. We here integrated human marrow-isolated adult multilineage-inducible (MIAMI) stem cells and pharmacologically active microcarriers (PAMs) into an injectable non-toxic silanized-hydroxypropyl methylcellulose (Si-HPMC) hydrogel. The goal is to obtain an injectable non-toxic cell and growth factor delivery device...
November 16, 2016: Acta Biomaterialia
https://www.readbyqxmd.com/read/27848099/dental-pulp-tissue-engineering-of-pulpotomized-rat-molars-with-bone-marrow-mesenchymal-stem-cells
#18
Takafumi Ito, Tomoatsu Kaneko, Yukiko Sueyama, Reika Kaneko, Takashi Okiji
The major goal of dental pulp tissue engineering is to enable the healing of inflamed tissue or to replace necrotic pulp tissue with newly formed dental pulp tissue. Here, we report a protocol for pulp tissue engineering in vivo in pulpotomized rat teeth using constructs of rat bone marrow mesenchymal stem cells, preformed biodegradable scaffolds, and hydrogel. The constructs were implanted into pulpotomized pulp chambers for 3, 7, or 14 days. At 3 days, cells were located mainly along the preformed scaffolds...
November 15, 2016: Odontology
https://www.readbyqxmd.com/read/27829016/delivery-of-human-adipose-stem-cells-spheroids-into-lockyballs
#19
Karina R Silva, Rodrigo A Rezende, Frederico D A S Pereira, Peter Gruber, Mellannie P Stuart, Aleksandr Ovsianikov, Ken Brakke, Vladimir Kasyanov, Jorge V L da Silva, José M Granjeiro, Leandra S Baptista, Vladimir Mironov
Adipose stem cells (ASCs) spheroids show enhanced regenerative effects compared to single cells. Also, spheroids have been recently introduced as building blocks in directed self-assembly strategy. Recent efforts aim to improve long-term cell retention and integration by the use of microencapsulation delivery systems that can rapidly integrate in the implantation site. Interlockable solid synthetic microscaffolds, so called lockyballs, were recently designed with hooks and loops to enhance cell retention and integration at the implantation site as well as to support spheroids aggregation after transplantation...
2016: PloS One
https://www.readbyqxmd.com/read/27826002/3d-culture-of-human-pluripotent-stem-cells-in-rgd-alginate-hydrogel-improves-retinal-tissue-development
#20
Nicola C Hunt, Dean Hallam, Ayesha Karimi, Carla B Mellough, Jinju Chen, David H W Steel, Majlinda Lako
: No treatments exist to effectively treat many retinal diseases. Retinal pigmented epithelium (RPE) and neural retina can be generated from human embryonic stem cells/induced pluripotent stem cells (hESCs/hiPSCs). The efficacy of current protocols is, however, limited. It was hypothesised that generation of laminated neural retina and/or RPE from hiPSCs/hESCs could be enhanced by three dimensional (3D) culture in hydrogels. hiPSC- and hESC-derived embryoid bodies (EBs) were encapsulated in 0...
November 5, 2016: Acta Biomaterialia
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