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Stem cell,hydrogel,scaffold

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https://www.readbyqxmd.com/read/28711686/hydrogel-elasticity-and-microarchitecture-regulate-dental-derived-mesenchymal-stem-cells-host-immune-system-cross-talk
#1
Sahar Ansari, Chider Chen, Mohammad Mahdi Hasani-Sarabadi, Bo Yu, Homayoun H Zadeh, Benjamin M Wu, Alireza Moshaverinia
The host immune system (T-lymphocytes and their pro-inflammatory cytokines) has been shown to compromise bone regeneration ability of mesenchymal stem cells (MSCs). We have recently shown that hydrogel, used as an encapsulating biomaterial affects the cross-talk among host immune cells and MSCs. However, the role of hydrogel elasticity and porosity in regulation of cross-talk between dental-derived MSCs and immune cells is unclear. In this study, we demonstrate that the modulus of elasticity and porosity of the scaffold influence T-lymphocyte-dental MSC interplay by regulating the penetration of inflammatory T cells and their cytokines...
July 12, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28699619/highly-defined-3d-printed-chitosan-scaffolds-featuring-improved-cell-growth
#2
Lisa Elviri, Ruben Foresti, Carlo Bergonzi, Francesca Zimetti, Cinzia Marchi, Annalisa Bianchera, Franco Bernini, Marco Silvestri, Ruggero Bettini
The augmented demand for medical devices devoted to tissue regeneration and possessing a controlled micro-architecture means there is a need for industrial scale-up in the production of hydrogels. A new 3D printing technique was applied to the automation of a freeze-gelation method for the preparation of chitosan scaffolds with controlled porosity. For this aim, a dedicated 3D printer was built in-house: a preliminary effort has been necessary to explore the printing parameter space to optimize the printing results in terms of geometry, tolerances and mechanical properties of the product...
July 12, 2017: Biomedical Materials
https://www.readbyqxmd.com/read/28699526/hydrogel-based-cell-therapies-for-kidney-regeneration-current-trends-in-biofabrication-and-in-vivo-repair
#3
Katja Jansen, Carl C L Schuurmans, Jitske Jansen, Rosalinde Masereeuw, Tina Vermonden
Facing the problems of limited renal regeneration capacity and the persistent shortage of donor kidneys, dialysis remains the only treatment option for many end-stage renal disease patients. Unfortunately, dialysis is only a medium-term solution because large and protein-bound uremic solutes are not efficiently cleared from the body and lead to disease progression over time. Current strategies for improved renal replacement therapies (RRTs) range from whole organ engineering to biofabrication of renal assist devices and biological injectables for in vivo regeneration...
July 10, 2017: Current Pharmaceutical Design
https://www.readbyqxmd.com/read/28698551/laminin-511-and-521-based-matrices-for-efficient-ex-vivo-expansion-of-human-limbal-epithelial-progenitor-cells
#4
Naresh Polisetti, Lydia Sorokin, Naoki Okumura, Noriko Koizumi, Shigeru Kinoshita, Friedrich E Kruse, Ursula Schlötzer-Schrehardt
Optimization of culture conditions for human limbal epithelial stem/progenitor cells (LEPC) that incorporate the in vivo cell-matrix interactions are essential to enhance LEPC ex vivo-expansion and transplantation efficiency. Here, we investigate the efficacy of laminin (LN) isoforms preferentially expressed in the limbal niche as culture matrices for epithelial tissue engineering. Analyses of expression patterns of LN chains in the human limbal niche provided evidence for enrichment of LN-α2, -α3, -α5, -β1, -β2, -β3, -γ1, -γ2 and -γ3 chains in the limbal basement membrane, with LN-α5 representing a signature component specifically produced by epithelial progenitor cells...
July 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28694033/drug-depot-anchoring-hydrogel-a-self-assembling-scaffold-for-localized-drug-release-and-enhanced-stem-cell-differentiation
#5
Ruixiang Li, Zhiqing Pang, Huining He, Seungjin Lee, Jing Qin, Jian Wu, Liang Pang, Jianxin Wang, Victor C Yang
Localized and long-term delivery of growth factors has been a long-standing challenge for stem cell-based tissue engineering. In the current study, a polymeric drug depot-anchoring hydrogel scaffold was developed for the sustained release of macromolecules to enhance the differentiation of stem cells. Self-assembling peptide (RADA16)-modified drug depots (RDDs) were prepared and anchored to a RADA16 hydrogel. The anchoring effect of RADA16 modification on the RDDs was tested both in vitro and in vivo. It was shown that the in vitro leakage of RDDs from the RADA16 hydrogel was significantly less than that of the unmodified drug depots (DDs)...
July 8, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28691691/development-of-a-clay-based-bioink-for-3d-cell-printing-for-skeletal-application
#6
Tilman Ahlfeld, Gianluca Cidonio, David Kilian, Sarah Duin, Ashwini Rahul Akkineni, Jon I Dawson, Shoufeng Yang, Anja Lode, Richard Oreffo, Michael Gelinsky
Three-dimensional printing of cell-laden hydrogels has evolved as a promising approach on the route to patient-specific or complex tissue engineered constructs. However, it is still challenging to print structures with both, high shape fidelity and cell vitality. Herein, we used a synthetic nanosilicate clay, called Laponite, to build up scaffolds utilizing the extrusion-based method 3D plotting. By blending with alginate and methylcellulose, a bioink was developed which allowed easy extrusion, achieving scaffolds with high printing fidelity...
July 10, 2017: Biofabrication
https://www.readbyqxmd.com/read/28691584/fibrin-gel-as-a-scaffold-for-photoreceptor-cells-differentiation-from-conjunctiva-mesenchymal-stem-cells-in-retina-tissue-engineering
#7
Mostafa Soleimannejad, Somayeh Ebrahimi-Barough, Masoud Soleimani, Samad Nadri, Seyed Mohammad Tavangar, Ramak Roohipoor, Meysam Yazdankhah, Neda Bayat, Mohammad Riazi-Esfahani, Jafar Ai
Stem cell-based therapies are attraction approaches for regenerative medicine for treating retinal diseases. One of the limitations in cell therapy is cell death following post-injection whit preventing functional integration with retinal tissue. Fibrin gel, a bio-polymeric material with excellent biocompatibility, provides numerous advantages as a tissue engineering scaffold and a stem cell carrier. Therefore, current research is focusing on developing fibrin hydrogel scaffolds to protect stem cells during delivery and to stimulate endogenous regeneration through interactions of transplanted stem cells and retinal tissue...
July 10, 2017: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/28684336/coculture-of-mesenchymal-stem-cells-and-endothelial-cells-enhances-host-tissue-integration-and-epidermis-maturation-through-akt-activation-in-gelatin-methacryloyl-hydrogel-based-skin-model
#8
Xiaofei Zhang, Jun Li, Pengxiang Ye, Guifang Gao, Karen Hubbell, Xiaofeng Cui
A major challenge for clinical use of skin substitutes is insufficient host tissue integration leading to loosening and partial necrosis of the implant. In this present study, a three-dimensional (3D) coculture system constructed using human umbilical cord mesenchymal stem cells (uc-MSCs) and umbilical vein endothelial cells (HUVECs) encapsulated in gelatin methacryloyl (GelMA) hydrogels was evaluated to determine the outcomes of cell-cell interactions in vitro and in vivo. The results revealed that GelMA hydrogels displayed minor cytotoxicity on both cell types...
July 3, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28677805/designer-d-form-self-assembling-peptide-scaffolds-promote-the-proliferation-and-migration-of-rat-bone-marrow-derived-mesenchymal-stem-cells
#9
Shuo Chen, Ao Zhou, Bin He, Weikang Zhao, Xiaojun Chen, Dianming Jiang
Self-assembling peptide (SAP) nanofiber hydrogel scaffolds have become increasingly important in tissue engineering due to their outstanding bioactivity and biodegradability. However, there is an initial concern on their long-term clinical use, since SAPs made of L-form amino acid sequences are sensitive to enzymatic degradation. In this study, we present a designer SAP, D-RADA16, made of all D-amino acid. We investigated the nanofiber morphology of D-RADA16, its potential for the culture of bone marrow-derived mesenchymal stem cells (BMSCs), and the proteolytic resistance of the biomaterial...
July 5, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28652926/silk-fibroin-hydroxyapatite-composite-hydrogel-induced-by-gamma-ray-irradiation-for-bone-tissue-engineering
#10
Min Hee Kim, Beom Su Kim, Jun Lee, Donghwan Cho, Oh Hyeong Kwon, Won Ho Park
BACKGROUND: In this study, silk fibroin (SF) composite hydrogels containing hydroxyapatite (HAP) nanoparticles (NPs) for bone tissue engineering were fabricated using gamma-ray (γ-ray) irradiation treatment. During the irradiation, the HAP dispersed SF solution was changed to the chemically crosslinked SF hydrogel. METHODS: Distribution of HAP NPs in the SF hydrogel was examined by SEM imagery and energy dispersive X-ray spectrophotometry, and the crystalline structure of SF composite hydrogels was also confirmed by X-ray diffractometry...
2017: Biomaterials Research
https://www.readbyqxmd.com/read/28639378/regulation-of-the-fate-of-dental-derived-mesenchymal-stem-cells-using-engineered-alginate-gelma-hydrogels
#11
Sahar Ansari, Patricia Sarrion, Mohammad Mahdi Hasani-Sadrabdi, Tara Aghaloo, Benjamin M Wu, Alireza Moshaverinia
Mesenchymal stem cells (MSCs) derived from dental and orofacial tissues provide an alternative therapeutic option for craniofacial bone tissue regeneration. However, there is still a need to improve stem cell delivery vehicles to regulate the fate of the encapsulated MSCs for high quality tissue regeneration. Matrix elasticity plays a vital role in MSC fate determination. Here we have prepared various hydrogel formulations based on alginate and gelatin methacryloyl (GelMA) and have encapsulated gingival mesenchymal stem cells (GMSCs) and human bone marrow MSCs (hBMMSCs) within these fabricated hydrogels...
June 22, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28634958/bioprinting-cartilage-tissue-from-mesenchymal-stem-cells-and-peg-hydrogel
#12
Guifang Gao, Karen Hubbell, Arndt F Schilling, Guohao Dai, Xiaofeng Cui
Bioprinting based on thermal inkjet printing is one of the most attractive enabling technologies for tissue engineering and regeneration. During the printing process, cells, scaffolds , and growth factors are rapidly deposited to the desired two-dimensional (2D) and three-dimensional (3D) locations. Ideally, the bioprinted tissues are able to mimic the native anatomic structures in order to restore the biological functions. In this study, a bioprinting platform for 3D cartilage tissue engineering was developed using a commercially available thermal inkjet printer with simultaneous photopolymerization ...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28629100/biomaterials-and-cells-for-cardiac-tissue-engineering-current-choices
#13
REVIEW
Rusha Chaudhuri, Madhumitha Ramachandran, Pearl Moharil, Megha Harumalani, Amit K Jaiswal
The major purpose of cardiac tissue engineering is to engineer cells on scaffolds and use it as a substitute to infarcted cardiac cells. With an ever-increasing risk of cardiac diseases there is an increasing need to have a stable and sustainable approach to cure such ailments. This review provides a comprehensive update on the cell sources and biomaterials essential for cardiac tissue engineering, ensuring their biocompatibility under a variety of conditions. Cells can be obtained from allogenic or autologous sources...
October 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28629085/design-of-a-hybrid-biomaterial-for-tissue-engineering-biopolymer-scaffold-integrated-with-an-autologous-hydrogel-carrying-mesenchymal-stem-cells
#14
Caroline R Weinstein-Oppenheimer, Donald I Brown, Rodrigo Coloma, Patricio Morales, Mauricio Reyna-Jeldes, María J Díaz, Elizabeth Sánchez, Cristian A Acevedo
Biologically active biomaterials as biopolymers and hydrogels have been used in medical applications providing favorable results in tissue engineering. In this research, a wound dressing device was designed by integration of an autologous clot hydrogel carrying mesenchymal stem-cells onto a biopolymeric scaffold. This hybrid biomaterial was tested in-vitro and in-vivo, and used in a human clinical case. The biopolymeric scaffold was made with gelatin, chitosan and hyaluronic acid, using a freeze-drying method...
October 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28629066/glucosamine-modified-polyethylene-glycol-hydrogel-mediated-chondrogenic-differentiation-of-human-mesenchymal-stem-cells
#15
Hang Yao, Jingchen Xue, Qunfang Wang, Renjian Xie, Weichang Li, Sa Liu, Jinglei Cai, Dajiang Qin, Dong-An Wang, Li Ren
Glucosamine (GA) is an important cartilage matrix precursor for the glycosaminoglycan biochemical synthesis, and has positive effects on cartilage regeneration, particularly in osteoarthritis therapy. However, it has not been used as a bioactive group in scaffolds for cartilage repair widely. In this study, we synthesized modified polyethylene glycol (PEG) hydrogel with glucosamine and then encapsulated human bone mesenchymal stem cells (hBMSCs) in the hydrogel to induce the differentiation of hBMSCs into chondrocytes in three-dimensional culture...
October 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28627740/biofabrication-of-soft-tissue-templates-for-engineering-the-bone-ligament-interface
#16
Ella Harris, Yurong Liu, Grainne Cuniffe, David Morrissey, Simon Carroll, Kevin Mulhall, Daniel J Kelly
Regenerating damaged tissue interfaces remains a significant clinical challenge, requiring recapitulation of the structure, composition and function of the native enthesis. In the ligament-to-bone interface this region transitions from ligament to fibrocartilage, to calcified cartilage and then to bone. This gradation in tissue types facilitates the transfer of load between soft and hard structures while minimizing stress concentrations at the interface. Previous attempts to engineer the ligament-bone interface have utilized various scaffold materials with an array of various cell types and/or biological cues...
June 19, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28611002/chondrogenesis-of-human-bone-marrow-mesenchymal-stem-cells-in-3-dimensional-photocrosslinked-hydrogel-constructs-effect-of-cell-seeding-density-and-material-stiffness
#17
Aaron X Sun, Hang Lin, Madalyn R Fritch, He Shen, Pete G Alexander, Michael DeHart, Rocky S Tuan
Three-dimensional hydrogel constructs incorporated with live stem cells that support chondrogenic differentiation and maintenance offer a promising regenerative route towards addressing the limited self-repair capabilities of articular cartilage. In particular, hydrogel scaffolds that augment chondrogenesis and recapitulate the native physical properties of cartilage, such as compressive strength, can potentially be applied in point-of-care procedures. We report here the synthesis of two new materials, [poly-l-lactic acid/polyethylene glycol/poly-l-lactic acid] (PLLA-PEG 1000) and [poly-d,l-lactic acid/polyethylene glycol/poly-d,l-lactic acid] (PDLLA-PEG 1000), that are biodegradable, biocompatible (>80% viability post fabrication), and possess high, physiologically relevant mechanical strength (∼1500 to 1800kPa)...
June 10, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28605908/chondroitin-sulfate-based-biomineralizing-surface-hydrogels-for-bone-tissue-engineering
#18
Hwan D Kim, Eunjee A Lee, Young-Hyeon An, Seunghyun L Kim, Seunghun S Lee, Seung Jung Yu, Hae Lin Jang, Ki Tae Nam, Sung Gap Im, Nathaniel S Hwang
Chondroitin sulfate (CS) is the major component of glycosaminoglycan in connective tissue. In this study, we fabricated methacrylated PEGDA/CS-based hydrogels with varying CS concentration (0, 1, 5, and 10%) and investigated them as biomineralizing three-dimensional scaffolds for charged ion binding and depositions. Due to its negative charge from the sulfate group, CS exhibited an osteogenically favorable microenvironment by binding charged ions such as calcium and phosphate. Particularly, ion binding and distribution within negatively charged hydrogel was dependent on CS concentration...
June 22, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28593744/thermosensitive-heparin-poloxamer-hydrogels-enhance-the-effects-of-gdnf-on-neuronal-circuit-remodeling-and-neuroprotection-after-spinal-cord-injury
#19
Ying-Zheng Zhao, Xi Jiang, Qian Lin, He-Lin Xu, Ya-Dong Huang, Cui-Tao Lu, Jun Cai
Traumatic spinal cord injury (SCI) results in paraplegia or quadriplegia, and currently, therapeutic interventions for axonal regeneration after SCI are not clinically available. Animal studies have revealed that glial cell-derived neurotrophic factor (GDNF) plays multiple beneficial roles in neuroprotection, glial scarring remodeling, axon regeneration and remyelination in SCI. However, the poor physicochemical stability of GDNF, as well as its limited ability to cross the blood-spinal cord barrier, hampers the development of GDNF as an effective therapeutic intervention in clinical practice...
June 7, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28585970/mesoporous-silica-nanoparticles-in-injectable-hydrogels-factors-influencing-cellular-uptake-and-viability
#20
Bernhard Baumann, Rainer Wittig, Mika Lindén
The incorporation of nanoparticles as drug vectors into 3D scaffolds has attracted a lot of recent interest. In particular, tissue engineering applications would benefit from a spatially and temporally regulated release of biological cues, which act on precursor/stem cells in a three-dimensional growth environment. Injectable cell- and nanoparticle-containing scaffolds are especially interesting in this respect, but require matrix self-assembly and coordinated interactions between cells, matrices, and nanoparticles, which are largely uncharacterized yet...
June 6, 2017: Nanoscale
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