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https://www.readbyqxmd.com/read/29691026/strengthening-injectable-thermo-sensitive-nipaam-g-chitosan-hydrogels-using-chemical-cross-linking-of-disulfide-bonds-as-scaffolds-for-tissue-engineering
#1
Shu-Wei Wu, Xifeng Liu, A Lee Miller, Yu-Shiuan Cheng, Ming-Long Yeh, Lichun Lu
In the present study, we fabricated non-toxic, injectable, and thermo-sensitive NIPAAm-g-chitosan (NC) hydrogels with thiol modification for introduction of disulfide cross-linking strategy. Previously, NIPAAm and chitosan copolymer has been proven to have excellent biocompatibility, biodegradability and rapid phase transition after injection, suitable to serve as cell carriers or implanted scaffolds. However, weak mechanical properties significantly limit their potential for biomedical fields. In order to overcome this issue, we incorporated thiol side chains into chitosan by covalently conjugating N-acetyl-cysteine (NAC) with carbodiimide chemistry to strengthen mechanical properties...
July 15, 2018: Carbohydrate Polymers
https://www.readbyqxmd.com/read/29687742/amniotic-membrane-and-its-epithelial-and-mesenchymal-stem-cells-as-an-appropriate-source-for-skin-tissue-engineering-and-regenerative-medicine
#2
Behrouz Farhadihosseinabadi, Mehrdad Farahani, Tahereh Tayebi, Ameneh Jafari, Felor Biniazan, Khashayar Modaresifar, Hamideh Moravvej, Soheyl Bahrami, Heinz Redl, Lobat Tayebi, Hassan Niknejad
One of the main goals of tissue engineering and regenerative medicine is to develop skin substitutes for treating deep dermal and full thickness wounds. In this regard, both scaffold and cell source have a fundamental role to achieve exactly the same histological and physiological analog of skin. Amnion epithelial and mesenchymal cells possess the characteristics of pluripotent stem cells which have the capability to differentiate into all three germ layers and can be obtained without any ethical concern. Amniotic cells also produce different growth factors, angio-modulatory cytokines, anti-bacterial peptides and a wide range of anti-inflammatory agents which eventually cause acceleration in wound healing...
April 24, 2018: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/29684624/component-effect-of-stem-cell-loaded-thermosensitive-polypeptide-hydrogels-on-cartilage-repair
#3
He Liu, Yilong Cheng, Jinjin Chen, Fei Chang, Jincheng Wang, Jianxun Ding, Xuesi Chen
Biophysical properties of the desired biomimetic scaffolds, such as porosity and elasticity, have been proven associated with the efficacy of cartilage regeneration. In this work, the copolymers of poly(L-alanine)-block-poly(ethylene glycol)-block-poly(L-alanine) (PA-PEG-PA) and poly(L-alanine-co-L-phenylalanine)-block-poly(ethylene glycol)-block-poly(L-alanine-co-L-phenylalanine) (PAF-PEG-PAF) with different ratios of alanine to phenylalanine were synthesized. The introduction of a hydrophobic amino acid, phenylalanine, into polyalanine-based thermosensitive hydrogel leaded to the enhanced gelation behaviors and upregulated mechanical properties...
April 20, 2018: Acta Biomaterialia
https://www.readbyqxmd.com/read/29668079/co-culture-of-adipose-derived-stem-cells-and-chondrocytes-on-three-dimensionally-printed-bioscaffolds-for-craniofacial-cartilage-engineering
#4
Robert J Morrison, Hassan B Nasser, Khaled N Kashlan, David A Zopf, Derek J Milner, Colleen L Flanangan, Matthew B Wheeler, Glenn E Green, Scott J Hollister
OBJECTIVES/HYPOTHESIS: Reconstruction of craniofacial cartilagenous defects are among the most challenging surgical procedures in facial plastic surgery. Bioengineered craniofacial cartilage holds immense potential to surpass current reconstructive options, but limitations to clinical translation exist. We endeavored to determine the viability of utilizing adipose-derived stem cell-chondrocyte co-culture and three-dimensional (3D) printing to produce 3D bioscaffolds for cartilage tissue engineering...
April 18, 2018: Laryngoscope
https://www.readbyqxmd.com/read/29667686/rheological-characterization-of-dynamic-remodeling-of-the-pericellular-region-by-human-mesenchymal-stem-cell-secreted-enzymes-in-well-defined-synthetic-hydrogel-scaffolds
#5
Maryam Daviran, Sarah M Longwill, Jonah F Casella, Kelly M Schultz
Human mesenchymal stem cells (hMSCs) dynamically remodel their microenvironment during basic processes, such as migration and differentiation. Migration requires extracellular matrix invasion, necessitating dynamic cell-material interactions. Understanding these interactions is critical to advancing materials designs that harness and manipulate these processes for applications including wound healing and tissue regeneration. In this work, we encapsulate hMSCs in a cell-degradable poly(ethylene glycol)-peptide hydrogel to determine how cell-secreted enzymes, specifically matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs), create unique pericellular microenvironments...
April 18, 2018: Soft Matter
https://www.readbyqxmd.com/read/29665386/enzymatically-crosslinked-and-mechanically-tunable-silk-fibroin-pullulan-hydrogels-for-mesenchymal-stem-cells-delivery
#6
Tao Li, Xiongbo Song, Changmei Weng, Xin Wang, Jiangyi Wu, Li Sun, Xiaoyuan Gong, Wei-Nan Zeng, Liu Yang, Cheng Chen
Hydrogels with good biocompatibility, proper degradation rates, and tissue-matched elasticity are widely used in tissue engineering, regenerative medicine, and drug delivery. In this study, enzymatically crosslinked biocompatible hydrogels were successfully developed using silk fibroin (SF) and pullulan (PL) under physiological conditions in the presence of both horseradish peroxidase (HRP) and hydrogen peroxide (H2 O2 ). A series of properties of the hydrogels including gelation time, equilibrium swelling, enzyme degradation, morphology, rheological property, and compression modulus of SF/PL hydrogels were studied by varying the concentration of PL...
April 14, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29663707/engineering-pro-regenerative-hydrogels-for-scarless-wound-healing
#7
REVIEW
Guoming Sun, Yu-I Shen, John W Harmon
Skin and skin appendages protect the body from harmful environment and prevent internal organs from dehydration. Superficial epidermal wounds usually heal without scarring, however, deep dermal wound healing commonly ends up with nonfunctioning scar formation with substantial loss of skin appendage. Wound healing is one of the most complex dynamic biological processes, during which a cascade of biomolecules combine with stem cell influx and matrix synthesis and synergistically contribute to wound healing at all levels...
April 16, 2018: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/29663706/patient-specific-bioinks-for-3d-bioprinting-of-tissue-engineering-scaffolds
#8
Negar Faramarzi, Iman K Yazdi, Mahboubeh Nabavinia, Andrea Gemma, Adele Fanelli, Andrea Caizzone, Leon M Ptaszek, Indranil Sinha, Ali Khademhosseini, Jeremy N Ruskin, Ali Tamayol
Bioprinting has emerged as a promising tool in tissue engineering and regenerative medicine. Various 3D printing strategies have been developed to enable bioprinting of various biopolymers and hydrogels. However, the incorporation of biological factors has not been well explored. As the importance of personalized medicine is becoming more clear, the need for the development of bioinks containing autologous/patient-specific biological factors for tissue engineering applications becomes more evident. Platelet-rich plasma (PRP) is used as a patient-specific source of autologous growth factors that can be easily incorporated to hydrogels and printed into 3D constructs...
April 16, 2018: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/29663702/assembly-of-peg-microgels-into-porous-cell-instructive-3d-scaffolds-via-thiol-ene-click-chemistry
#9
Shangjing Xin, Omar M Wyman, Daniel L Alge
The assembly of microgel building blocks into 3D scaffolds is an emerging strategy for tissue engineering. A key advantage is that the inherent microporosity of these scaffolds provides cells with a more permissive environment than conventional nanoporous hydrogels. Here, norbornene-bearing poly(ethylene glycol) (PEG) based microgels are assembled into 3D cell-instructive scaffolds using a PEG-dithiol linker and thiol-ene click photopolymerization. The bulk modulus of these materials depends primarily on the crosslink density of the microgel building blocks...
April 16, 2018: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/29661055/messenger-rna-delivery-for-tissue-engineering-and-regenerative-medicine-applications
#10
Siddharth Patel, Avathamsa Athirasala, Paula Dos Passos Menezes, Narayanan Ashwanikumar, Ting Zou, Gaurav Sahay, Luiz Bertassoni
The ability to control cellular processes and precisely direct cellular reprogramming has revolutionized regenerative medicine. Recent advances in in vitro transcribed (IVT) mRNA technology with chemical modifications have led to development of methods that control spatio-temporal gene expression. Additionally, there is a current thrust towards the development of safe, integration-free approaches to gene therapy for translational purposes. In this review, we describe strategies of synthetic IVT mRNA modifications and non-viral technologies for intracellular delivery...
April 17, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29659011/immune-system-augmentation-via-humanization-using-stem-progenitor-cells-and-bioengineering-in-a-breast-cancer-model-study
#11
Abbas Shafiee, Jacqui A McGovern, Christoph A Lahr, Christoph Meinert, Davide Moi, Ferdinand Wagner, Marietta Landgraf, Elena De-Juan-Pardo, Roberta Mazzieri, Dietmar W Hutmacher
Despite significant advances, most current in vivo models fail to fully recapitulate the biological processes that occur in humans. Here we aimed to develop an advanced humanized model with features of an organ bone by providing different bone tissue cellular compartments including pre-osteoblasts, mesenchymal stem/stromal (MSCs), endothelial and hematopoietic cells in an engineered microenvironment. The bone compartment was generated by culturing the human MSCs, umbilical vein endothelial cells with gelatin methacryloyl hydrogels in the centre of a melt-electrospun polycaprolactone tubular scaffolds which were seeded with human pre-osteoblasts...
April 16, 2018: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/29656479/fiber-length-and-concentration-synergistic-effect-on-mechanical-and-cellular-response-in-wet-laid-poly-lactic-acid-fibrous-scaffolds
#12
Andrew T Wood, Dominique Everett, Sanjay Kumar, Manoj K Mishra, Vinoy Thomas
In the area of biomaterials, fibers not only offer increased mechanical response, but also serve as an extracellular matrix mimicking morphology to direct cellular attachment and proliferation. While biologically similar in morphology, soft, and flexible hydrogel materials have low mechanical properties. For applications in tissue engineering, the lack of directional cues and attachment regions within the biogels is undesired as cells require a guide for adequate attachment and organized proliferation. In this work, we have investigated the role of poly(lactic acid) (PLA) fiber length and concentration as a reinforcement phase in a gelatin hydrogel matrix and the resultant mechanical and cellular responses...
April 15, 2018: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/29651947/transplantation-of-bdnf-gene-recombinant-mesenchymal-stem-cells-and-adhesive-peptide-modified-hydrogel-scaffold-for-spinal-cord-repair
#13
Li-Ming Li, Ling-Ling Huang, Xin-Chi Jiang, Jia-Chen Chen, Hong-Wei OuYang, Jian-Qing Gao
Mesenchymal stem cells (MSCs) are promising candidates for nerve tissue engineering. Brain derived neurotrophic factor (BDNF) secreted by MSCs can function to increase neural differentiation and relieve inflammation response. Gene transfection technology is an efficient strategy to increase the secretion levels of cytokines and enhance cellular functions. However, transfection and in vivo gene expression of environmentally sensitive stem cells have been one of the most challenging subjects due to the requirement in both safety and transfection efficiency...
April 13, 2018: Current Gene Therapy
https://www.readbyqxmd.com/read/29651746/human-neural-stem-progenitor-cells-derived-from-epileptic-human-brain-in-a-self-assembling-peptide-nanoscaffold-improve-traumatic-brain-injury-in-rats
#14
Ali Jahanbazi Jahan-Abad, Sajad Sahab Negah, Hassan Hosseini Ravandi, Sedigheh Ghasemi, Maryam Borhani-Haghighi, Walter Stummer, Ali Gorji, Maryam Khaleghi Ghadiri
Traumatic brain injury (TBI) is a disruption in the brain functions following a head trauma. Cell therapy may provide a promising treatment for TBI. Among different cell types, human neural stem cells cultured in self-assembling peptide scaffolds have been suggested as a potential novel method for cell replacement treatment after TBI. In the present study, we accessed the effects of human neural stem/progenitor cells (hNS/PCs) derived from epileptic human brain and human adipose-derived stromal/stem cells (hADSCs) seeded in PuraMatrix hydrogel (PM) on brain function after TBI in an animal model of brain injury...
April 12, 2018: Molecular Neurobiology
https://www.readbyqxmd.com/read/29644098/hydrogel-based-scaffolds-to-support-intrathecal-stem-cell-transplantation-as-a-gateway-to-the-spinal-cord-clinical-needs-biomaterials-and-imaging-technologies
#15
REVIEW
J Miguel Oliveira, Luisa Carvalho, Joana Silva-Correia, Sílvia Vieira, Malgorzata Majchrzak, Barbara Lukomska, Luiza Stanaszek, Paulina Strymecka, Izabela Malysz-Cymborska, Dominika Golubczyk, Lukasz Kalkowski, Rui L Reis, Miroslaw Janowski, Piotr Walczak
The prospects for cell replacement in spinal cord diseases are impeded by inefficient stem cell delivery. The deep location of the spinal cord and complex surgical access, as well as densely packed vital structures, question the feasibility of the widespread use of multiple spinal cord punctures to inject stem cells. Disorders characterized by disseminated pathology are particularly appealing for the distribution of cells globally throughout the spinal cord in a minimally invasive fashion. The intrathecal space, with access to a relatively large surface area along the spinal cord, is an attractive route for global stem cell delivery, and, indeed, is highly promising, but the success of this approach relies on the ability of cells (1) to survive in the cerebrospinal fluid (CSF), (2) to adhere to the spinal cord surface, and (3) to migrate, ultimately, into the parenchyma...
2018: NPJ Regenerative Medicine
https://www.readbyqxmd.com/read/29627677/preparation-of-high-bioactivity-multilayered-bone-marrow-mesenchymal-stem-cell-sheet-for-myocardial-infarction-using-a-3d-dynamic-system
#16
Yingwei Wang, Jianhua Zhang, Zixi Qin, Zepei Fan, Cheng Lu, Baoxin Chen, Jupeng Zhao, Xiaojuan Li, Fei Xiao, Xi Lin, Zheng Wu
Cell sheet techniques offer a promising future for myocardial infarction (MI) therapy; however, insufficient nutrition supply remains the major limitation in maintaining stem cell bioactivities in vitro. In order to enhance cell sheet mechanical strength and bioactivities, a decellularized porcine pericardium (DPP) scaffold was prepared by the phospholipase A2 method, and aspartic acid was used as a spacer arm to improve the vascular endothelial growth factor crosslink efficiency on the DPP scaffold. Based on this scaffold, multilayered bone marrow mesenchymal stem cell sheets were rapidly constructed, using RAD16-I peptide hydrogel as a temporary 3D scaffold, and cell sheets were cultured in either the 3D-dynamic system (DCcs) or the traditional static condition (SCcs)...
April 5, 2018: Acta Biomaterialia
https://www.readbyqxmd.com/read/29626700/combinatory-approach-for-developing-silk-fibroin-scaffolds-for-cartilage-regeneration
#17
Viviana P Ribeiro, Alain da Silva Morais, F Raquel Maia, Raphael F Canadas, João B Costa, Ana L Oliveira, Joaquim M Oliveira, Rui L Reis
Several processing technologies and engineering strategies have been combined to create scaffolds with superior performance for efficient tissue regeneration. Cartilage tissue is a good example of that, presenting limited self-healing capacity together with a high elasticity and load-bearing properties. In this work, novel porous silk fibroin (SF) scaffolds derived from horseradish peroxidase (HRP)-mediated crosslinking of highly concentrated aqueous SF solution (16 wt.%) in combination with salt-leaching and freeze-drying methodologies were developed for articular cartilage tissue engineering (TE) applications...
April 4, 2018: Acta Biomaterialia
https://www.readbyqxmd.com/read/29621597/mesenchymal-stem-cells-encapsulated-into-biomimetic-hydrogel-scaffold-gradually-release-ccl2-chemokine-in-situ-preserving-cytoarchitecture-and-promoting-functional-recovery-in-spinal-cord-injury
#18
S Papa, I Vismara, A Mariani, M Barilani, S Rimondo, M De Paola, N Panini, E Erba, E Mauri, F Rossi, G Forloni, L Lazzari, P Veglianese
Spinal cord injury (SCI) is an acute neurodegenerative disorder caused by traumatic damage of the spinal cord. The neuropathological evolution of the primary trauma involves multifactorial processes that exacerbate the pathology, worsening the neurodegeneration and limiting neuroregeneration. This complexity suggests that multi-therapeutic approaches, rather than any single treatment, might be more effective. Encouraging preclinical results indicate that stem cell-based treatments may improve the disease outcome due to their multi-therapeutic ability...
April 2, 2018: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/29609508/dynamical-release-nanospheres-containing-cell-growth-factor-from-biopolymer-hydrogel-via-reversible-covalent-conjugation
#19
Bowen Ren, Xueyun Chen, Ye Ma, Shoukang Du, Saibo Qian, Yongjie Xu, Zhilin Yan, Jianliang Li, Yang Jia, Huaping Tan, Zhonghua Ling, Yong Chen, Xiaohong Hu
For practical adipose regeneration, the challenge is to dynamically deliver the key adipogenic insulin-like growth factors in hydrogels to induce adipogenesis. In order to achieve dynamic release, smart hydrogels to sense the change in the blood glucose concentration is required when glucose concentration increases. In this study, a heparin-based hydrogel has been developed for use in dynamic delivery of heparin nanospheres containing insulin-like growth factor. The gel scaffold was facilely prepared in physiological conditions by the formation of boronate-maltose ester cross-links between boronate and maltose groups of heparin derivatives...
April 3, 2018: Journal of Biomaterials Science. Polymer Edition
https://www.readbyqxmd.com/read/29576458/encapsulation-of-mesenchymal-stem-cells-in-chitosan-%C3%AE-glycerophosphate-hydrogel-for-seeding-on-a-novel-calcium-phosphate-cement-scaffold
#20
Tao Liu, Jian Li, Zengwu Shao, Kaige Ma, Zhicai Zhang, Baichuan Wang, Yannan Zhang
Due to its moldability, biocompatibility, osteoconductivity and resorbability, calcium phosphate cement (CPC) is a highly promising scaffold material for orthopedic applications. However, pH changes and ionic activity during the CPC setting reaction may adversely affect cells seeded directly on CPC. Moreover, a lack of macropores in CPC limits ingrowth of new bone. The objectives of this study were to prepare macroporous CPC scaffolds via porogen leaching, using mannitol crystals as the porogen and to evaluate the in vitro proliferation and osteogenic differentiation of mesenchymal stem cells (MSCs) encapsulated in chitosan/β-glycerophosphate (C/GP) hydrogel prior to exposure to the novel CPC scaffold...
March 22, 2018: Medical Engineering & Physics
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