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Stem cell,hydrogel,scaffold

Wei Zhu, Haitao Cui, Benchaa Boualam, Fahed Masood, Erin Flynn, Raj Rao, Zhiyong Zhang, Lijie Grace Zhang
Cartilage tissue is prone to degradation and has little capacity for self-healing due to its avascularity. Tissue engineering, which provides artificial scaffolds to repair injured tissues, is a novel and promising strategy for cartilage repair. 3D bioprinting offers even greater potential for repairing degenerative tissue by simultaneously integrating living cells, biomaterials, and biological cues to provide a customized scaffold. With regard to cell selection, mesenchymal stem cells (MSCs) hold great capacity for differentiating into a variety of cell types, including chondrocytes, and could therefore be utilized as a cartilage cell source in 3D bioprinting...
February 15, 2018: Nanotechnology
Binata Joddar, Nishat Tasnim, Vikram Thakur, Alok Kumar, Richard W McCallum, Munmun Chattopadhyay
Gastroparesis (GP) is associated with depletion of interstitial cells of Cajal (ICCs) and enteric neurons, which leads to pyloric dysfunction followed by severe nausea, vomiting and delayed gastric emptying. Regenerating these fundamental structures with mesenchymal stem cell (MSC) therapy would be helpful to restore gastric function in GP. MSCs have been successfully used in animal models of other gastrointestinal (GI) diseases, including colitis. However, no study has been performed with these cells on GP animals...
February 7, 2018: Bioengineering
Khairy M Tohamy, Mostafa Mabrouk, Islam E Soliman, Hanan H Beherei, Mohamed A Aboelnasr
Sodium alginate (SA)/hydroxyethylcellulose (HEC)/hydroxyapatite (HA) composite scaffolds were explored for enhanced in vitro bone regeneration. The SA/HEC/HA composites were synthesized using the lyophilization technique and further cross-linked in the presence of calcium ions to form composite hydrogel networks. The physicochemical, thermal behavior and morphology properties of the prepared scaffolds were characterized through XRD, DSC/TGA, FTIR and SEM. Furthermore, the mechanical behavior of the under investigated scaffolds was determined using texture analyzer...
January 30, 2018: International Journal of Biological Macromolecules
Jun Yin, Mengling Yan, Yancheng Wang, Jianzhong Fu, Hairui Suo
Methacrylated gelatin (GelMA) has been widely used as the tissue engineered scaffold material, but only low concentration GelMA hydrogels were found to be promising cell-laden bioinks with excellent cell viability. In this work, we reported a strategy for precisely deposition of 5% (w/v) cell-laden GelMA bioinks into controlled micro-architectures with high cell viability using extrusion-based three-dimensional (3D) bioprinting. By adding gelatin into GelMA bioinks, a two-step crosslinking combining the rapid and reversible thermo-crosslinking of gelatin with irreversible photo-crosslinking of GelMA was achieved...
February 6, 2018: ACS Applied Materials & Interfaces
Lili Zhang, Haowei Fang, Kunxi Zhang, Jingbo Yin
Stem cell shape appears to be involved in the regulation of osteogenesis, which has been confirmed in two dimensional surfaces and three dimensional hydrogels. The present study was to evaluate the effect of matrix-controlled cellular shape on osteogenesis in three dimensional porous scaffolds based on the sodium alginate (ALG) and chitosan (CS). Three ALG/CS scaffolds, especially including a stiff one, were fabricated from different precursor matrices. Soft scaffold A was fabricated from ALG/CS polyelectrolyte, and further cross-linked by Ca2+ and glutaraldehyde to achieve soft scaffold B with alterative hydrophilicity...
February 2, 2018: ACS Applied Materials & Interfaces
Emanuele Mauri, Alessandro Sacchetti, Nunzio Vicario, Luca Peruzzotti-Jametti, Filippo Rossi, Stefano Pluchino
The use of neural stem cells (NSCs) in cell therapy has become a powerful tool used for the treatment of central nervous system diseases, including traumatic brain and spinal cord injuries. However, a significant drawback is related to the limited viability after transplantation in situ. The design of three-dimensional (3D) scaffolds that are capable of resembling the architecture and physico-chemical features of an extracellular environment could be a suitable approach to improve cell survival and preserve their cellular active phase over time...
January 25, 2018: Biomaterials Science
Hao Cheng, Rosa Chabok, Xiaofei Guan, Aditya Chawla, Yuxiao Li, Ali Khademhosseini, Hae Lin Jang
The inorganic part of human bone is mainly composed of hydroxyapatite (HAP: Ca10(PO4)6(OH)2) and whitlockite (WH: Ca18Mg2(HPO4)2(PO4)12) minerals, where the WH phase occupies up to 20-35% of total weight. These two bone minerals have different crystal structures and physicochemical properties, implying their distinguished role in bone physiology. However, until now, the biological significance of the presence of a certain ratio between HAP and WH in bone is unclear. To address this fundamental question, bone mimetic scaffolds are designed to encapsulate human mesenchymal stem cells (MSCs) for assessing their osteogenic activity depending on different ratios of HAP and WH...
January 20, 2018: Acta Biomaterialia
Ivan Hernandez, Alok Kumar, Binata Joddar
In this study, a hybrid system consisting of 3D printed polycaprolactone (PCL) filled with hydrogel was developed as an application for reconstruction of long bone defects, which are innately difficult to repair due to large missing segments of bone. A 3D printed gyroid scaffold of PCL allowed a larger amount of hydrogel to be loaded within the scaffolds as compared to 3D printed mesh and honeycomb scaffolds of similar volumes and strut thicknesses. The hydrogel was a mixture of alginate, gelatin, and nano-hydroxyapatite, infiltrated with human mesenchymal stem cells (hMSC) to enhance the osteoconductivity and biocompatibility of the system...
September 2017: Gels
Lisa Rödling, Esther Magano Volz, Annamarija Raic, Katharina Brändle, Matthias Franzreb, Cornelia Lee-Thedieck
There is an urgent need for 3D cell culture systems that avoid the oversimplifications and artifacts of conventional culture in 2D. However, 3D culture within the cavities of porous biomaterials or large 3D structures harboring high cell numbers is limited by the needs to nurture cells and to remove growth-limiting metabolites. To overcome the diffusion-limited transport of such soluble factors in 3D culture, mixing can be improved by pumping, stirring or shaking, but this in turn can lead to other problems...
January 19, 2018: Advanced Healthcare Materials
Richard A Que, Janahan Arulmoli, Nancy A Da Silva, Lisa A Flanagan, Szu-Wen Wang
Adhesion to the microenvironment profoundly affects stem cell functions, including proliferation and differentiation, and understanding the interaction of stem cells with the microenvironment is important for controlling their behavior. In this study, we investigated the effects of the integrin binding epitopes GFOGER and IKVAV (natively present in collagen I and laminin, respectively) on human neural stem/progenitor cells (hNSPCs). To test the specificity of these epitopes, GFOGER or IKVAV were placed within the context of recombinant triple-helical collagen III engineered to be devoid of native integrin binding sites...
January 17, 2018: Journal of Biomedical Materials Research. Part A
Zexing Yan, Heyong Yin, Michael Nerlich, Christian G Pfeifer, Denitsa Docheva
BACKGROUND: Tendons are dense connective tissues and critical components for the integrity and function of the musculoskeletal system. Tendons connect bone to muscle and transmit forces on which locomotion entirely depends. Due to trauma, overuse and age-related degeneration, many people suffer from acute or chronic tendon injuries. Owing to their hypovascularity and hypocellularity, tendinopathies remain a substantial challenge for both clinicians and researchers. Surgical treatment includes suture or transplantation of autograft, allograft or xenograft, and these serve as the most common technique for rescuing tendon injuries...
January 5, 2018: Journal of Experimental Orthopaedics
Xiaomin Wang, Jimin Zhang, Weilong Cui, Yuan Fang, Li Li, Shenglu Ji, Duo Mao, Tingyu Ke, Xin Yao, Dan Ding, Guowei Feng, Deling Kong
Stem cell treatment for critical limb ischemia yields a limited therapeutic effect due to cell loss and dysfunction caused by local ischemic environment. Biomimetic scaffolds emerge as ideal cell delivery vehicles for regulating cell fate via mimicking the components of stem cell niche. Herein, we prepared a bioactive hydrogel by mixing chitosan and hyaluronic acid that is immobilized with C domain peptide of IGF-1 (IGF-1C), and examined whether this hydrogel could augment stem cell survival and therapeutic potential...
January 12, 2018: ACS Applied Materials & Interfaces
Stuart A Young, Stephen E Sherman, Tyler T Cooper, Cody Brown, Fraz Anjum, David A Hess, Lauren E Flynn, Brian G Amsden
A promising strategy for treating peripheral ischemia involves the delivery of stem cells to promote angiogenesis through paracrine signaling. Treatment success depends on cell localization, retention, and survival within the mechanically dynamic intramuscular (IM) environment. Herein we describe an injectable, in situ-gelling hydrogel for the IM delivery of adipose-derived stem/stromal cells (ASCs), specifically designed to withstand the dynamic loading conditions of the lower limb and facilitate cytokine release from encapsulated cells...
January 4, 2018: Biomaterials
Avathamsa Athirasala, Anthony Tahayeri, Greeshma Thrivikraman, Cristiane M França, Nelson Monteiro, Victor Tran, Jack Ferracane, Luiz E Bertassoni
Recent studies in tissue engineering have adopted extracellular matrix (ECM) derived scaffolds as natural and cytocompatible microenvironments for tissue regeneration. The dentin matrix, specifically, has been shown to be associated with a host of soluble and insoluble signaling molecules that can promote odontogenesis. Here, we have developed a novel bioink, blending printable alginate (3% w/v) hydrogels with the soluble and insoluble fractions of the dentin matrix. We have optimized the printing parameters and the concentrations of the individual components of the bioink for print accuracy, cell viability and odontogenic potential...
January 10, 2018: Biofabrication
James Devon Tang, Kyle J Lampe
An active area of research in the field of regenerative medicine involves the development of bioactive matrices that can promote cellular interactions and elicit desirable regenerative behavior in vivo. This is particularly important in the context of ischemic stroke where a focal lesion forms preventing the regrowth of brain tissue. Protein-based molecules have been used as building blocks to create supramolecular structures that emulate the properties of the native healthy extracellular matrix (ECM) within the central nervous system (CNS)...
January 3, 2018: Biomedical Materials
Byeong-Ju Kwon, Xintong Wang, Mi-Lan Kang, Jin You, Shin-Jeong Lee, Won Shik Kim, Young-Sup Yoon, Jong-Chul Park, Hak-Joon Sung
Stem cells are a promising cell source for regenerative medicine due to their differentiation and self-renewal capacities. In the field of regenerative medicine and tissue engineering, a variety of biomedical technologies have been tested to improve proangiogenic activities of stem cells. However, their therapeutic effect is found to be limited in the clinic because of cell loss, senescence, and insufficient therapeutic activities. To address this type of issue, advanced techniques for biomaterial synthesis and fabrication have been approached to mimic proangiogenic microenvironment and to direct proangiogenic activities...
December 29, 2017: Macromolecular Bioscience
Tianyi Wang, Fan Yang
BACKGROUND: Mesenchymal stem cells (MSCs) hold great promise for cartilage repair given their relative abundance, ease of isolation, and chondrogenic potential. To enhance MSC chondrogenesis, extracellular matrix components can be incorporated into three-dimensional (3D) scaffolds as an artificial cell niche. Chondroitin sulfate (CS)-containing hydrogels have been shown to support 3D chondrogenesis, but the effects of varying CS concentration and hydrogel stiffness on 3D MSC chondrogenesis remains elusive...
December 19, 2017: Stem Cell Research & Therapy
Peter Apelgren, Matteo Amoroso, Anders Lindahl, Camilla Brantsing, Nicole Rotter, Paul Gatenholm, Lars Kölby
Cartilage repair and replacement is a major challenge in plastic reconstructive surgery. The development of a process capable of creating a patient-specific cartilage framework would be a major breakthrough. Here, we described methods for creating human cartilage in vivo and quantitatively assessing the proliferative capacity and cartilage-formation ability in mono- and co-cultures of human chondrocytes and human mesenchymal stem cells in a three-dimensional (3D)-bioprinted hydrogel scaffold. The 3D-bioprinted constructs (5 × 5 × 1...
2017: PloS One
Ajay Tijore, Scott Alexander Irvine, Udi Sarig, Priyadarshini Mhaisalkar, Vrushali Baisane, Subbu S Venkatraman
Here, we have developed a 3D bioprinted microchanneled gelatin hydrogel that promotes human mesenchymal stem cell (hMSC) myocardial commitment and supports native cardiomyocytes (CMs) contractile functionality. Firstly, we studied the effect of bioprinted microchanneled hydrogel on the alignment, elongation, and differentiation of hMSC. Notably, the cells displayed well defined F-actin anisotropy and elongated morphology on the microchanneled hydrogel, hence showing the effects of topographical control over cell behavior...
December 13, 2017: Biofabrication
Micaela Pannella, Luciana Giardino, Laura Calzà, Mercedes Fernández
In this chapter we illustrate protocols to investigate growth and neurotrophic factors in human and rodent (rat and mouse)-derived embryonic stem cells. The conventional two-dimensional cell monolayer system to grow embryonic stem cells is presented, focusing on the coating strategies also using extracellular matrix components. Then, different approaches for three-dimensional stem cell culture are presented, using hydrogels and scaffolds. Quantitative polymerase chain reaction, immunocytochemistry, immunoenzymatic ELISA assay, and multiparametric assays to quantify growth and neurotrophic factor production are presented...
2018: Methods in Molecular Biology
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