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Stem cell,hydrogel,scaffold

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https://www.readbyqxmd.com/read/29046917/effects-of-an-injectable-functionalized-self-assembling-nanopeptide-hydrogel-on-angiogenesis-and-neurogenesis-for-regeneration-of-the-central-nervous-system
#1
Tzu-Wei Wang, Kai-Chieh Chang, Liang-Hsin Chen, Shih-Yung Liao, Chia-Wei Yeh, Yung-Jen Chuang
Brain injury is a devastating medical condition and represents a major health problem. Tissue and organ reconstruction have been regarded as promising therapeutic strategies. Here, we propose a regenerative methodology focusing on the provision of functionalized nanopeptide scaffolds to facilitate angiogenesis and neurogenesis at the brain injury site. The peptide RADA16-SVVYGLR undergoes self-assembly to construct an interconnected network with intertwining nanofibers, and can be controlled to display various physicochemical properties by the adjustment of microenvironmental factors such as pH and ion concentration...
October 19, 2017: Nanoscale
https://www.readbyqxmd.com/read/29042614/3d-printed-poly-%C3%AE%C2%B5-caprolactone-scaffold-integrated-with-cell-laden-chitosan-hydrogels-for-bone-tissue-engineering
#2
Liang Dong, Shao-Jie Wang, Xin-Rong Zhao, Yu-Fang Zhu, Jia-Kuo Yu
Synthetic polymeric scaffolds are commonly used in bone tissue engineering (BTE) due to their biocompatibility and adequate mechanical properties. However, their hydrophobicity and the lack of specific cell recognition sites confined their practical application. In this study, to improve the cell seeding efficiency and osteoinductivity, an injectable thermo-sensitive chitosan hydrogel (CSG) was incorporated into a 3D-printed poly(ε-caprolactone) (PCL) scaffold to form a hybrid scaffold. To demonstrate the feasibility of this hybrid system for BTE application, rabbit bone marrow mesenchymal stem cells (BMMSCs) and bone morphogenetic protein-2 (BMP-2) were encapsulated in CSG...
October 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29027711/expansion-of-mesenchymal-stem-cells-on-electrospun-scaffolds-maintains-stemness-mechano-responsivity-and-differentiation-potential
#3
Su-Jin Heo, Spencer E Szczesny, Dong Hwa Kim, Kamiel S Saleh, Robert L Mauck
Mesenchymal stem cells (MSCs) hold great promise for regenerative therapies and tissue engineering applications given their multipotential differentiation capacity. However, MSC isolation and expansion are typically performed on super-physiologically stiff tissue culture plastic (TCP), which may alter their behavior and lead to unintended consequences upon implantation. In contrast, electrospun nanofibrous scaffolds possess physical and mechanical properties that are similar to that of native tissue. In this study, we investigated whether isolation and expansion of juvenile bovine MSCs directly onto electrospun nanofibrous scaffolds better preserves MSC phenotype and stemness compared to TCP...
October 13, 2017: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://www.readbyqxmd.com/read/29022693/a-tri-layer-three-dimensional-hydrogel-composite-scaffold-containing-encapsulated-adipose-derived-stem-cells-promotes-bladder-reconstruction-via-sdf-1%C3%AE-cxcr4-pathway
#4
Dongdong Xiao, Hao Yan, Qiong Wang, Xiangguo Lv, Ming Zhang, Yang Zhao, Zhe Zhou, Jiping Xu, Qian Sun, Kang Sun, Wei Li, Mujun Lu
Bladder acellular matrix graft-alginate di-aldehyde-gelatin hydrogel-silk mesh (BAMG-HS) encapsulated with adipose-derived stem cells (ASCs) was evaluated in a rat model of augmentation cystoplasty, including BAMG-HS-ASCs (n = 18, subgroup n = 6 for 2, 4 and 12 weeks), acellular BAMG-HS (n = 6 for 12 weeks) and cystotomy control (n = 6 for 12 weeks) groups. Equipped with good cytocompatibility and superior mechanical properties (elastic modulus: 5.33 ± 0.96 MPa, maximum load: 28.90 ± 0.69 N), BAMG-HS acted a tri-layer "sandwich" scaffold with minimal interference in systemic homeostasis...
October 12, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29017117/characterisation-of-hyaluronic-acid-methylcellulose-hydrogels-for-3d-bioprinting
#5
Nicholas Law, Brandon Doney, Hayley Glover, Yahua Qin, Zachary M Aman, Timothy B Sercombe, Lawrence J Liew, Rodney J Dilley, Barry J Doyle
Hydrogels containing hyaluronic acid (HA) and methylcellulose (MC) have shown promising results for three dimensional (3D) bioprinting applications. However, several parameters influence the applicability bioprinting and there is scarce data in the literature characterising HAMC. We assessed eight concentrations of HAMC for printability, swelling and stability over time, rheological and structural behaviour, and viability of mesenchymal stem cells. We show that HAMC blends behave as viscous solutions at 4°C and have faster gelation times at higher temperatures, typically gelling upon reaching 37°C...
September 28, 2017: Journal of the Mechanical Behavior of Biomedical Materials
https://www.readbyqxmd.com/read/28975732/a-novel-calcium-accumulating-peptide-gelatin-in-situ-forming-hydrogel-for-enhanced-bone-regeneration
#6
Beom Soo Jo, Yunki Lee, Jin Sook Suh, Yoon Shin Park, Hyun Jung Lee, Jue-Yeon Lee, Jaejin Cho, Gene Lee, Chong Pyoung Chung, Ki Dong Park, Yoon Jeong Park
Bioactive agents, including proteins and peptides, can be loaded into hydrogels to improve bone regenerative capacity with their controlled release. However, the current loading method has focused on physical mixing, which has limited release control. Therefore, alternative conjugation of bioactive agents with hydrogels is highly recommended. Direct chemical conjugation of synthetic peptides containing a functional moiety with a hydrogel would be ideal. Here, we synthesized a bioactive calcium accumulating peptide (CAP) containing a collagen binding motif (CBM), which can induce osteogenic differentiation...
October 4, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28974254/synergistic-effects-on-mesenchymal-stem-cell-based-cartilage-regeneration-by-chondrogenic-preconditioning-and-mechanical-stimulation
#7
Sien Lin, Wayne Yuk Wai Lee, Qian Feng, Liangliang Xu, Bin Wang, Gene Chi Wai Man, Yuanfeng Chen, Xiaohua Jiang, Liming Bian, Liao Cui, Bo Wei, Gang Li
BACKGROUND: Mesenchymal stem cells (MSCs) hold promising translational potential in cartilage regeneration. However, the efficacy of MSC-based tissue engineering is not satisfactory in the treatment of cartilage defect because of the inevitable cellular functional changes during ex vivo cell expansion. How to maintain the chondrogenic capacity of MSCs to improve their therapeutic outcomes remains an outstanding question. METHODS: Bone marrow-derived MSCs were firstly primed in chondrogenic induction medium which was then replaced with normal growth medium to attain the manipulated cells (M-MSCs)...
October 3, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28972602/thermo-rheological-responsive-microcapsules-for-time-dependent-controlled-release-of-human-mesenchymal-stromal-cells
#8
W C Mak, B Magne, K Y Cheung, D Atanasova, M Griffith
Human mesenchymal stromal cells (hMSCs) are adult-source cells that have been extensively evaluated for cell-based therapies. hMSCs delivered by intravascular injection have been reported to accumulate at the sites of injury to promote tissue repair and can also be employed as vectors for the delivery of therapeutic genes. However, the full potential of hMSCs remains limited as the cells are lost after injection due to anoikis and the adverse pathologic environment. Encapsulation of cells has been proposed as a means of increasing cell viability...
October 3, 2017: Biomaterials Science
https://www.readbyqxmd.com/read/28969895/three-dimensional-hydrogel-culture-conditions-promote-the-differentiation-of-human-induced-pluripotent-stem-cells-into-hepatocytes
#9
Ying Luo, Cheng Lou, Sui Zhang, Zhengyan Zhu, Qianzhe Xing, Peng Wang, Tong Liu, Hui Liu, Chenglong Li, Wenxia Shi, Zhi Du, Yingtang Gao
BACKGROUND AIMS: Human induced pluripotent stem cells (hiPSCs) are becoming increasingly popular in research endeavors due to their potential for clinical application; however, such application is challenging due to limitations such as inferior function and low induction efficiency. In this study, we aimed to establish a three-dimensional (3D) culture condition to mimic the environment in which hepatogenesis occurs in vivo to enhance the differentiation of hiPSCs for large-scale culture and high throughput BAL application...
September 29, 2017: Cytotherapy
https://www.readbyqxmd.com/read/28959819/hydration-of-hydrogels-regulates-vascularization-in-vivo
#10
Jie Wu, Kunxi Zhang, Xi Yu, Jianxun Ding, Lei Cui, Jingbo Yin
The key barrier to the clinical application of tissue engineering scaffolds is the limitation of rapid and sufficient vascularization. Adipose-derived stem cells (ASCs), especially multicellular aggregates, exhibited a promising angiogenic activity. Herein, we designed a series of poly(l-glutamic acid) (PLGA)-based hydrogels with tunable hydration to control the in situ formation of multicellular spheroids. Oligo(ethylene glycol)s (OEGs) were employed to regulate the hydration of hydrogels. The hydrogel cross-linked with ethylene glycol (OEG1) supported the most excellent adhesion and proliferation of human ASCs in vitro...
September 29, 2017: Biomaterials Science
https://www.readbyqxmd.com/read/28951745/in-vivo-articular-cartilage-regeneration-using-human-dental-pulp-stem-cells-cultured-in-an-alginate-scaffold-a-preliminary-study
#11
Manuel Mata, Lara Milian, Maria Oliver, Javier Zurriaga, Maria Sancho-Tello, Jose Javier Martin de Llano, Carmen Carda
Osteoarthritis is an inflammatory disease in which all joint-related elements, articular cartilage in particular, are affected. The poor regeneration capacity of this tissue together with the lack of pharmacological treatment has led to the development of regenerative medicine methodologies including microfracture and autologous chondrocyte implantation (ACI). The effectiveness of ACI has been shown in vitro and in vivo, but the use of other cell types, including bone marrow and adipose-derived mesenchymal stem cells, is necessary because of the poor proliferation rate of isolated articular chondrocytes...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28934875/natural-type-ii-collagen-hydrogel-fibrin-sealant-and-adipose-derived-stem-cells-as-a-promising-combination-for-articular-cartilage-repair
#12
Mariana Lazarini, Pedro Bordeaux-Rego, Renata Giardini-Rosa, Adriana S S Duarte, Mariana Ozello Baratti, Alessandro Rozim Zorzi, João Batista de Miranda, Carlos Lenz Cesar, Ângela Luzo, Sara Teresinha Olalla Saad
Objective Articular cartilage is an avascular tissue with limited ability of self-regeneration and the current clinical treatments have restricted capacity to restore damages induced by trauma or diseases. Therefore, new techniques are being tested for cartilage repair, using scaffolds and/or stem cells. Although type II collagen hydrogel, fibrin sealant, and adipose-derived stem cells (ASCs) represent suitable alternatives for cartilage formation, their combination has not yet been investigated in vivo for focal articular cartilage defects...
October 2017: Cartilage
https://www.readbyqxmd.com/read/28901689/prevascularization-of-3d-printed-bone-scaffolds-by-bioactive-hydrogels-and-cell-co-culture
#13
Mitchell A Kuss, Shaohua Wu, Ying Wang, Jason B Untrauer, Wenlong Li, Jung Yul Lim, Bin Duan
Vascularization is a fundamental prerequisite for large bone construct development and remains one of the main challenges of bone tissue engineering. Our current study presents the combination of 3D printing technique with a hydrogel-based prevascularization strategy to generate prevascularized bone constructs. Human adipose derived mesenchymal stem cells (ADMSC) and human umbilical vein endothelial cells (HUVEC) were encapsulated within our bioactive hydrogels, and the effects of culture conditions on in vitro vascularization were determined...
September 13, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28885199/adipogenic-differentiation-of-human-adipose-derived-mesenchymal-stem-cells-in-3d-architectured-gelatin-based-hydrogels-arcgel
#14
Jie Zou, Weiwei Wang, Axel T Neffe, Xun Xu, Zhengdong Li, Zijun Deng, Xianlei Sun, Nan Ma, Andreas Lendlein
Polymeric matrixes mimicking multiple function of the ECM are expected to enable a material induced regeneration of tissues. Here, we investigated the adipogenic differentiation of human adipose derived mesenchymal stem cells (hADSCs) in a 3D architectured gelatin based hydrogel (ArcGel) prepared from gelatin and L-lysine diisocyanate ethyl ester (LDI) in an one-step process, in which the formation of an open porous morphology and the chemical network formation were integrated. The ArcGel was designed to support adipose tissue regeneration with its 3D porous structure, high cell biocompatibility and mechanical properties compatible with human subcutaneous adipose tissue...
September 4, 2017: Clinical Hemorheology and Microcirculation
https://www.readbyqxmd.com/read/28884519/gelatin-methacrylate-scaffold-for-bone-tissue-engineering-the-influence-of-polymer-concentration
#15
Nehar Celikkin, Simone Mastrogiacomo, Jakub Jaroszewicz, X Frank Walboomers, Wojciech Swieszkowski
Gelatin Methacrylate (GelMA) is an inexpensive, photocrosslinkable, cell-responsive hydrogel which has drawn attention for a wide range of tissue engineering applications. The potential of GelMA scaffolds was demonstrated to be tunable for different TE applications through modifying the polymer concentration, methacrylation degree or UV light intensity. Despite the promising results of GelMA hydrogels in tissue engineering, the influence of polymer concentration for bone tissue engineering scaffolds was not established yet...
September 7, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28879659/extracellular-matrix-particle-glycosaminoglycan-composite-hydrogels-for-regenerative-medicine-applications
#16
Vince Beachley, Garret Ma, Chris Papadimitriou, Matt Gibson, Michael Corvelli, Jennifer Elisseeff
Tissue extracellular matrix (ECM) is a complex material made up of fibrous proteins and ground substance (glycosaminoglycans, GAGs) that are secreted by cells. ECM contains important biological cues that modulate cell behaviors, and it also serves as a structural scaffold to which cells can adhere. For clinical applications, where immune rejection is a constraint, ECM can be processed using decellularization methods intended to remove cells and donor antigens from tissue or organs, while preserving native biological cues essential for cell growth and differentiation...
September 6, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28876908/three-dimensional-electroconductive-hyaluronic-acid-hydrogels-incorporated-with-carbon-nanotubes-and-polypyrrole-by-catechol-mediated-dispersion-enhance-neurogenesis-of-human-neural-stem-cells
#17
Jisoo Shin, Eun Jung Choi, Jung Ho Cho, Ann-Na Cho, Yoonhee Jin, Kisuk Yang, Changsik Song, Seung-Woo Cho
Electrically conductive hyaluronic acid (HA) hydrogels incorporated with single-walled carbon nanotubes (CNTs) and/or polypyrrole (PPy) were developed to promote differentiation of human neural stem/progenitor cells (hNSPCs). The CNT and PPy nanocomposites, which do not easily disperse in aqueous phases, dispersed well and were efficiently incorporated into catechol-functionalized HA (HA-CA) hydrogels by the oxidative catechol chemistry used for hydrogel cross-linking. The prepared electroconductive HA hydrogels provided dynamic, electrically conductive three-dimensional (3D) extracellular matrix environments that were biocompatible with hNSPCs...
September 26, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28875574/rgd-functionalized-polyethylene-glycol-hydrogels-support-proliferation-and-in-vitro-chondrogenesis-of-human-periosteum-derived-cells
#18
Abhijith K Kudva, Frank P Luyten, Jennifer Patterson
The combination of progenitor cells with appropriate scaffolds and in vitro culture regimes is a promising area of research in bone and cartilage tissue engineering. Mesenchymal stem cells (MSCs), when encapsulated within hydrogels composed of the necessary cues and/or preconditioned using suitable culture conditions, have been shown to differentiate into bone or cartilage. Here, we utilized human periosteum-derived cells (hPDCs), a progenitor cell population with MSC characteristics, paired with protease-degradable, functionalized polyethylene glycol (PEG) hydrogels to create tissue-engineered constructs...
September 5, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28859902/nitric-oxide-releasing-hydrogel-promotes-endothelial-differentiation-of-mouse-embryonic-stem-cells
#19
Yan Nie, Kaiyue Zhang, Shuaiqiang Zhang, Dan Wang, Zhibo Han, Yongzhe Che, Deling Kong, Qiang Zhao, Zhongchao Han, Zuo-Xiang He, Na Liu, Fengxia Ma, Zongjin Li
Transplantation of endothelial cells (ECs) holds great promise for treating various kinds of ischemic diseases. However, the major challenge in ECs-based therapy in clinical applications is to provide high quality and enough amounts of cells. In this study, we developed a simple and efficient system to direct endothelial differentiation of mouse embryonic stem cells (ESCs) using a controllable chitosan nitric oxide (NO)-releasing hydrogel (CS-NO). ESCs were plated onto the hydrogel culture system, and the expressions of differentiation markers were measured...
August 28, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28844179/noninvasive-absolute-electron-paramagnetic-resonance-oxygen-imaging-for-the-assessment-of-tissue-graft-oxygenation
#20
Mrignayani Kotecha, Boris Epel, Sriram Ravindran, Deborah Dorcemus, Syam Nukavarapu, Howard Halpern
Oxygen is the single most important molecule for sustaining life and, therefore, an important variable in tissue engineering and regenerative medicine. It has been shown that the change in oxygen concentration in an artificial or tissue-engineered graft affects cell survival, differentiation, and tissue growth in profound ways. However, at present, there are no reliable methods to map partial oxygen pressure (pO2) in growing artificial tissues. Here, we adapt and test the suitability of electron paramagnetic resonance oxygen imaging (EPROI) in assessing tissue graft oxygenation in vitro...
October 12, 2017: Tissue Engineering. Part C, Methods
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