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Lipid Classification

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https://www.readbyqxmd.com/read/27902290/halodesulfovibrio-spirochaetisodalis-gen-nov-sp-nov-and-reclassification-of-four-desulfovibrio-spp
#1
Shivani Y, Subhash Y, Sasikala Ch, Ramana Ch V
An antibiotic producing, obligate anaerobic, Gram-stain-negative, catalase and oxidase negative strain (JC271T) isolated from a marine habitat was identified based on 16S rRNA gene sequences analysis as a new member in the family Desulfovibrionaceae. The closest phylogenetic members of strain JC271T were found to be Desulfovibrio marinisediminis C/L2T (99.2%), Desulfovibrio acrylicus W218T (98.7%), Desulfovibrio desulfuricans subsp. aestuarii (98.6%), Desulfovibrio oceani subsp. oceani (98.0%), Desulfovibrio oceani subsp...
October 16, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27902269/description-of-candidatus-marispirochaeta-associata-and-reclassification-of-spirochaeta-bajacaliforniensis-spirochaeta-smaragdinae-and-spirochaeta-sinaica-to-a-new-genus-sediminispirochaeta-gen-nov-as-sediminispirochaeta-bajacaliforniensis-comb-nov-sediminispirochaeta
#2
Shivani Y, Subhash Y, Sasikala Ch, Ramana Ch V
Strain JC231 was isolated from a coastal saline habitat of Gujarat and was identified based on 16S rRNA gene sequence analysis as a member belonging to the genus Spirochaeta and showed highest sequence similarity (<91%) with Spirochaeta bajacaliforniensis DSM 16054T and other members of the family Spirochaetaceae. Intensive attempts to culture strain JC231 in pure culture has failed and was associated with only one species of a Desulfovibrio. However, presence of fosmidomycin inhibited the growth of Desulfovibrio sp...
October 5, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27902262/saccharedens-versatilis-gen-nov-sp-nov-a-sugar-degrading-member-of-the-burkholderiales-isolated-from-cephalotes-rohweri-ant-guts
#3
Jonathan Y Lin, William J Hobson, John T Wertz
Cephalotes "turtle" ants host a core group of gut-associated symbionts, but their potential contributions to ant nutrition and disease resistance remain uncharacterized in vitro. To gain a better understanding of the metabolic capability of core symbionts belonging to the Burkholderiales, we cultivated and characterized strain CAG32T, isolated from the guts of Cephalotes rohweri ants. Strain CAG32T was rod-shaped, Gram-negative, motile, and formed pale-white colonies on trypticase soy agar. Optimum growth occurred under an atmosphere of 20% O2 supplemented with 1% CO2...
November 9, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27902249/microbacterium-sorbitolivorans-sp-nov-a-novel-member-of-microbacteriaceae-isolated-from-fermentingbed-in-pigpen
#4
Man Cai, Yu-Chen Meng, Hong-Can Liu, Lei-Lei Yang, Ying-Qian Kang, Yu-Guang Zhou
Two bacterial strains SZDIS-1-1T and GZDIS-1-1 were isolated from fermentingbed of a pigpen in Fujian Province, China. Cells were Gram-stain-positive, facultatively anaerobic, short-rods without flagellum. Their nearest phylogenetic neighbors were Microbacterium amylolyticum N5T (16S rRNA gene sequences similarity 98.2 %), Microbacterium indicum BBH6T (97.9 %) and Microbacterium gubbeenense DPC 5286T (97.8 %) with DNA-DNA hybridization values to strain SZDIS-1-1T as 20.0±1.2 %, 14.3±5.8 % and 19.1±1.6 %, respectively...
October 9, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27885557/isolation-of-paenibacillus-tumbae-sp-nov-from-the-tomb-of-the-emperor-yang-of-the-sui-dynasty-and-emended-description-of-the-genus-paenibacillus
#5
Zhi Huang, Fei Zhao, Yong-Hui Li
A novel strain, designated strain CSA42(T), was isolated from the tomb of emperor Yang of Sui in Yangzhou, Jiangsu province, China. Strain CSA42(T) was observed to be Gram-stain positive, strictly aerobic, rod-shaped, spore-forming and motile. The optimum conditions for growth were found to be 30 °C, pH 8.0 and without NaCl. Phylogenetic analysis, based on 16S rRNA gene sequences, revealed strain CSA42(T) to be closely related to Paenibacillus larvae DSM 7030(T) (94.7%), Paenibacillus doosanensis CAU 1055(T) (94...
November 24, 2016: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/27883040/effects-of-meja-on-arabidopsis-metabolome-under-endogenous-ja-deficiency
#6
Jingjing Cao, Mengya Li, Jian Chen, Pei Liu, Zhen Li
Jasmonates (JAs) play important roles in plant growth, development and defense. Comprehensive metabolomics profiling of plants under JA treatment provides insights into the interaction and regulation network of plant hormones. Here we applied high resolution mass spectrometry based metabolomics approach on Arabidopsis wild type and JA synthesis deficiency mutant opr3. The effects of exogenous MeJA treatment on the metabolites of opr3 were investigated. More than 10000 ion signals were detected and more than 2000 signals showed significant variation in different genotypes and treatment groups...
November 24, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27881864/mass-spectrometry-imaging-a-novel-technology-in-rheumatology
#7
REVIEW
Beatriz Rocha, Cristina Ruiz-Romero, Francisco J Blanco
Mass spectrometry imaging (MSI) is used to determine the relative abundance and spatial distribution of biomolecules such as peptides, proteins, lipids and other organic compounds in tissue sections by their molecular masses. This technique provides a sensitive and label-free approach for high-resolution imaging, and is currently used in an increasing number of biomedical applications such as biomarker discovery, tissue classification and drug monitoring. Owing to technological advances in the past 5 years in diverse MSI strategies, this technology is expected to become a standard tool in clinical practice and provides information complementary to that obtained using existing methods...
November 24, 2016: Nature Reviews. Rheumatology
https://www.readbyqxmd.com/read/27876264/intravascular-ultrasound-and-near-infrared-spectroscopic-characterization-of-thin-cap-fibroatheroma
#8
Shinji Inaba, Gary S Mintz, Allen P Burke, Gregg W Stone, Renu Virmani, Mitsuaki Matsumura, Rupa Parvataneni, Rishi Puri, Stephen J Nicholls, Akiko Maehara
Thin-cap fibroatheromas (TCFAs) are considered precursors for plaque rupture and subsequent acute coronary events. We investigated intravascular ultrasound (IVUS) and near-infrared spectroscopy (NIRS) characteristics of lesions that were histopathologic TCFAs. IVUS, NIRS, and histopathology were performed in 271 atherosclerotic lesions from 107 fresh coronary arteries from 54 patients at necropsy. The plaque burden and remodeling index calculated by IVUS and maximum lipid core burden index within any 4-mm segment (maxLCBI4mm) calculated by NIRS were compared among each plaque type based on histopathologic classifications but focusing on TCFA...
October 31, 2016: American Journal of Cardiology
https://www.readbyqxmd.com/read/27874199/glucose-and-lipids-levels-with-lanreotide-autogel-120-mg-in-treatment-na%C3%A3-ve-patients-with-acromegaly-data-from-the-primarys-study
#9
Philippe J Caron, Stephan Petersenn, Aude Houchard, Caroline Sert, John S Bevan
OBJECTIVE: Impaired glycaemic control, characteristic of acromegaly, can be exacerbated by treatment with somatostatin analogues (SSAs), particularly those with multireceptor activity. We present data from the PRIMARYS study on the impact of the SSA lanreotide, associated with tumour volume and hormonal improvements, on glucose and other metabolic parameters in acromegaly. DESIGN: PRIMARYS was a 48-week open-label single-arm phase-3b study of lanreotide autogel 120mg/4 weeks...
November 22, 2016: Clinical Endocrinology
https://www.readbyqxmd.com/read/27867716/automatic-classification-of-atherosclerotic-plaques-imaged-with-intravascular-oct
#10
Jose J Rico-Jimenez, Daniel U Campos-Delgado, Martin Villiger, Kenichiro Otsuka, Brett E Bouma, Javier A Jo
Intravascular optical coherence tomography (IV-OCT) allows evaluation of atherosclerotic plaques; however, plaque characterization is performed by visual assessment and requires a trained expert for interpretation of the large data sets. Here, we present a novel computational method for automated IV-OCT plaque characterization. This method is based on the modeling of each A-line of an IV-OCT data set as a linear combination of a number of depth profiles. After estimating these depth profiles by means of an alternating least square optimization strategy, they are automatically classified to predefined tissue types based on their morphological characteristics...
October 1, 2016: Biomedical Optics Express
https://www.readbyqxmd.com/read/27862147/signal-preprocessing-multivariate-analysis-and-software-tools-for-ma-ldi-tof-mass-spectrometry-imaging-for-biological-applications
#11
REVIEW
Pere Ràfols, Dídac Vilalta, Jesús Brezmes, Nicolau Cañellas, Esteban Del Castillo, Oscar Yanes, Noelia Ramírez, Xavier Correig
Mass spectrometry imaging (MSI) is a label-free analytical technique capable of molecularly characterizing biological samples, including tissues and cell lines. The constant development of analytical instrumentation and strategies over the previous decade makes MSI a key tool in clinical research. Nevertheless, most MSI studies are limited to targeted analysis or the mere visualization of a few molecular species (proteins, peptides, metabolites, or lipids) in a region of interest without fully exploiting the possibilities inherent in the MSI technique, such as tissue classification and segmentation or the identification of relevant biomarkers from an untargeted approach...
November 9, 2016: Mass Spectrometry Reviews
https://www.readbyqxmd.com/read/27849101/-emergency-management-of-intoxications-in-the-dog-and-cat
#12
Esther Hassdenteufel, Hendrik Lehmann, Matthias Schneider, Andreas Moritz
Intoxications may lead to life-threatening emergencies. While stabilising the patient, therapy should focus on a rapid and effective elimination of the toxin. General measures for decontamination (gastrointestinal, dermal, ocular) aim to effectively decrease the absorption of the poisonous substance. Further classification of the substance and its properties can assist with the choice of specific treatment options, including dialysis and specific antidotal therapy. In recent years, treatment with lipid emulsions for enhanced elimination of lipophilic substances has become an important therapeutic option...
November 16, 2016: Tierärztliche Praxis. Ausgabe K, Kleintiere/Heimtiere
https://www.readbyqxmd.com/read/27831900/aggregate-penetrance-of-genomic-variants-for-actionable-disorders-in-european-and-african-americans
#13
Pradeep Natarajan, Nina B Gold, Alexander G Bick, Heather McLaughlin, Peter Kraft, Heidi L Rehm, Gina M Peloso, James G Wilson, Adolfo Correa, Jonathan G Seidman, Christine E Seidman, Sekar Kathiresan, Robert C Green
In populations that have not been selected for family history of disease, it is unclear how commonly pathogenic variants (PVs) in disease-associated genes for rare Mendelian conditions are found and how often they are associated with clinical features of these conditions. We conducted independent, prospective analyses of participants in two community-based epidemiological studies to test the hypothesis that persons carrying PVs in any of 56 genes that lead to 24 dominantly inherited, actionable conditions are more likely to exhibit the clinical features of the corresponding diseases than those without PVs...
November 9, 2016: Science Translational Medicine
https://www.readbyqxmd.com/read/27810772/fourier-transform-infra-red-spectroscopic-signatures-for-lung-cells-epithelial-mesenchymal-transition-a-preliminary-report
#14
Atasi Sarkar, Sanghamitra Sengupta, Anirban Mukherjee, Jyotirmoy Chatterjee
Infra red (IR) spectral characterization can provide label-free cellular metabolic signatures of normal and diseased circumstances in a rapid and non-invasive manner. Present study endeavoured to enlist Fourier transform infra red (FTIR) spectroscopic signatures for lung normal and cancer cells during chemically induced epithelial mesenchymal transition (EMT) for which global metabolic dimension is not well reported yet. Occurrence of EMT was validated with morphological and immunocytochemical confirmation...
October 17, 2016: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/27806246/crystal-liquid-fugacity-ratio-as-a-surrogate-parameter-for-intestinal-permeability
#15
Parvin Zakeri-Milani, Zohreh Fasihi, Jafar Akbari, Ensieh Jannatabadi, Mohammad Barzegar-Jalali, Raimar Loebenberg, Hadi Valizadeh
BACKGROUND: We assessed the feasibility of using crystal-liquid fugacity ratio (CLFR) as an alternative parameter for intestinal permeability in the biopharmaceutical classification (BCS) of passively absorbed drugs. METHODS: Dose number, fraction of dose absorbed, intestinal permeability, and intrinsic dissolution rate were used as the input parameters. CLFR was determined using thermodynamic parameters i.e., melting point, molar fusion enthalpy, and entropy of drug molecules obtained using differential scanning calorimetry...
July 2016: Journal of Pharmacy & Pharmaceutical Sciences: a Publication of the Canadian Society for Pharmaceutical Sciences
https://www.readbyqxmd.com/read/27806083/integrated-multiple-omics-data-reveal-subtypes-of-hepatocellular-carcinoma
#16
Gang Liu, Chuanpeng Dong, Lei Liu
Hepatocellular carcinoma is one of the most heterogeneous cancers, as reflected by its multiple grades and difficulty to subtype. In this study, we integrated copy number variation, DNA methylation, mRNA, and miRNA data with the developed "cluster of cluster" method and classified 256 HCC samples from TCGA (The Cancer Genome Atlas) into five major subgroups (S1-S5). We observed that this classification was associated with specific mutations and protein expression, and we detected that each subgroup had distinct molecular signatures...
2016: PloS One
https://www.readbyqxmd.com/read/27801954/the-who-2016-classification-of-testicular-non-germ-cell-tumours-a-review-and-update-from-the-international-society-of-urological-pathology-testis-consultation-panel
#17
REVIEW
Muhammad T Idrees, Thomas M Ulbright, Esther Oliva, Robert H Young, Rodolfo Montironi, Lars Egevad, Daniel Berney, John R Srigley, Jonathan I Epstein, Satish K Tickoo
The World Health Organization (WHO) released a new tumour classification for the genitourinary system in early 2016 after consensus by pathologists with expertise in these organs. It utilized the framework of the 2004 classification and incorporated the most up-to-date information concerning these tumours. In testicular tumours, the majority of the changes occurred in the nomenclature and classification of the germ cell tumours; however, several modifications were also initiated for the non-germ cell tumours...
November 1, 2016: Histopathology
https://www.readbyqxmd.com/read/27797726/effects-of-high-intensity-interval-training-on-cardiometabolic-health-a-systematic-review-and-meta-analysis-of-intervention-studies
#18
Romeo B Batacan, Mitch J Duncan, Vincent J Dalbo, Patrick S Tucker, Andrew S Fenning
The current review clarifies the cardiometabolic health effects of high-intensity interval training (HIIT) in adults. A systematic search (PubMed) examining HIIT and cardiometabolic health markers was completed on 15 October 2015. Sixty-five intervention studies were included for review and the methodological quality of included studies was assessed using the Downs and Black score. Studies were classified by intervention duration and body mass index classification. Outcomes with at least 5 effect sizes were synthesised using a random-effects meta-analysis of the standardised mean difference (SMD) in cardiometabolic health markers (baseline to postintervention) using Review Manager 5...
October 20, 2016: British Journal of Sports Medicine
https://www.readbyqxmd.com/read/27791073/nanomechanical-mechanism-for-lipid-bilayer-damage-induced-by-carbon-nanotubes-confined-in-intracellular-vesicles
#19
Wenpeng Zhu, Annette von dem Bussche, Xin Yi, Yang Qiu, Zhongying Wang, Paula Weston, Robert H Hurt, Agnes B Kane, Huajian Gao
Understanding the behavior of low-dimensional nanomaterials confined in intracellular vesicles has been limited by the resolution of bioimaging techniques and the complex nature of the problem. Recent studies report that long, stiff carbon nanotubes are more cytotoxic than flexible varieties, but the mechanistic link between stiffness and cytotoxicity is not understood. Here we combine analytical modeling, molecular dynamics simulations, and in vitro intracellular imaging methods to reveal 1D carbon nanotube behavior within intracellular vesicles...
October 17, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/27790428/glycaemia-status-lipid-profile-and-renal-parameters-in-progressive-diabetic-neuropathy
#20
Debahuti Mohapatra, Komaladevi Sampath Damodar
INTRODUCTION: Diabetic Peripheral Neuropathy (DPN) is a common complication of diabetes. Existence of systemic co-morbidities in DPN patients has not been studied much, especially in Indian population. AIM: To evaluate glycaemic status, lipid profile, renal parameters and blood count to assess occurrence of co-morbidities as severity of DPN progresses. MATERIALS AND METHODS: A case control study involving 104 DPN patients and 43 controls of age 31-70years were selected...
September 2016: Journal of Clinical and Diagnostic Research: JCDR
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