Read by QxMD icon Read

Lipid Classification

Ji Ye Lee, Na Hyun Park, Wonwoong Lee, Eun Hye Kim, Young Ho Jin, Eun Kyung Seo, Jongki Hong
A comprehensive profiling method was established for the determination of various chemicals in Pinellia (P.) ternata and pedatisecta species. The profiling method comprises a fast ultrasonic extraction with various solvents, followed by GC-MS and LC-APCI-MS analysis. A total of 73 polar components as trimethylsilyl (TMS) derivatives were detected in methanol extract by GC-MS. The main components of the P. species were profiled as several kinds of fatty acids, amino acids, nucleic acids, carbohydrates, and phenolic compounds...
October 13, 2016: Journal of Chromatography. A
Hyo Myung Kim, Youngsub Eom, Jong Suk Song
Meibomian gland dysfunction (MGD) is a leading cause of dry eye disease (DED). Meibomian gland dysfunction is divided into two major categories based on meibomian gland secretion: low delivery and high delivery. The low-delivery states are further classified as either hyposecretory or obstructive subtype, and the high-delivery state is termed "hypersecretory MGD." Two parameters are commonly used to evaluate meibomian gland function: lipid layer thickness and meibum quality and expressibility. To evaluate the morphology of meibomian glands, meibography is used to detect meibomian gland dropout...
October 13, 2016: Eye & Contact Lens
Beatriz Rocha, Francisco J Blanco, Cristina Ruiz-Romero
Mass spectrometry imaging (MSI) is a technique used to visualize the spatial distribution of biomolecules such as peptides, proteins, lipids or other organic compounds by their molecular masses. Among the different MSI strategies, MALDI-MSI provides a sensitive and label-free approach for imaging of a wide variety of protein or peptide biomarkers from the surface of tissue sections, being currently used in an increasing number of biomedical applications such as biomarker discovery and tissue classification...
October 11, 2016: Biochimica et Biophysica Acta
P Benjamin Stranges, Mirkó Palla, Sergey Kalachikov, Jeff Nivala, Michael Dorwart, Andrew Trans, Shiv Kumar, Mintu Porel, Minchen Chien, Chuanjuan Tao, Irina Morozova, Zengmin Li, Shundi Shi, Aman Aberra, Cleoma Arnold, Alexander Yang, Anne Aguirre, Eric T Harada, Daniel Korenblum, James Pollard, Ashwini Bhat, Dmitriy Gremyachinskiy, Arek Bibillo, Roger Chen, Randy Davis, James J Russo, Carl W Fuller, Stefan Roever, Jingyue Ju, George M Church
Scalable, high-throughput DNA sequencing is a prerequisite for precision medicine and biomedical research. Recently, we presented a nanopore-based sequencing-by-synthesis (Nanopore-SBS) approach, which used a set of nucleotides with polymer tags that allow discrimination of the nucleotides in a biological nanopore. Here, we designed and covalently coupled a DNA polymerase to an α-hemolysin (αHL) heptamer using the SpyCatcher/SpyTag conjugation approach. These porin-polymerase conjugates were inserted into lipid bilayers on a complementary metal oxide semiconductor (CMOS)-based electrode array for high-throughput electrical recording of DNA synthesis...
October 11, 2016: Proceedings of the National Academy of Sciences of the United States of America
Yaira Barranco-Ruiz, Jeronimo Aragón-Vela, Cristina Casals, Antonio Martínez-Amat, Rafael A Casuso, Jesus R Huertas
OBJECTIVE: Controlling antioxidant supplementation in athletes involved in studies related to oxidative stress and muscle damage is the key to ensure results. The aim of this study was to confirm through high-performance liquid chromatography (HPLC) analysis whether well-trained individuals lied during a personal interview when asked if they were taking supplements with antioxidants, and how this could affect oxidative stress, muscle damage, and antioxidant response. METHODS: A total of 94 men, well trained in endurance sports, volunteered in this study...
July 28, 2016: Nutrition
Laura Mourino-Alvarez, Montserrat Baldan-Martin, Laura Gonzalez-Calero, Carlos Martinez-Laborde, Tamara Sastre-Oliva, Rafael Moreno-Luna, Luis F Lopez-Almodovar, Pedro L Sanchez, Francisco Fernandez-Aviles, Fernando Vivanco, Luis R Padial, Finn Akerstrom, Gloria Alvarez-Llamas, Fernando de la Cuesta, María G Barderas
BACKGROUND: The most common valve diseases are calcific aortic stenosis (AS) and aortic regurgitation (AR). The former is characterized by thickening of valve leaflets followed by progressive calcification, which produces progressive aortic valve (AV) narrowing, increased pressure afterload on the left ventricle (LV) and subsequent LV hypertrophy. On the other hand, AR is due to malcoaptation of the valve leaflets with resultant diastolic reflux of blood from aorta back to the LV producing volume and pressure overload and progressive LV dilatation...
September 26, 2016: International Journal of Cardiology
Sha Li, Xi Zhao, Yan Zhang, Cheng-Gang Zhu, Yuan-Lin Guo, Na-Qiong Wu, Rui-Xia Xu, Ping Qing, Ying Gao, Jing Sun, Geng Liu, Qian Dong, Jian-Jun Li
Plasma levels of proprotein convertase subtilisin/kexin type 9 (PCSK9), apolipoprotein C-III (apoC3) and small dense low density lipoprotein cholesterol (sdLDL-C), have been recently recognized as circulating atherosclerosis-related lipid measurements. We aimed to elucidate their associations with current dyslipidemias, and identify their levels at increased risk to dyslipidemia. A total of 1,605 consecutive, non-treated patients undergoing diagnostic/interventional coronary angiography were examined. Plasma PCSK9 and apoC3 levels were determined using a validated ELISA assay, and sdLDL-C was measured by the Lipoprint LDL System...
October 4, 2016: Oncotarget
Hansaem Choe, Ji-Yun Hwang, Jin A Yun, Ji-Myung Kim, Tae-Jin Song, Namsoo Chang, Yong-Jae Kim, Yuri Kim
BACKGROUND/OBJECTIVES: This study was conducted to examine relationships between dietary habits and intakes of antioxidants and B vitamins and the risk of ischemic stroke, and to compare dietary factors according to the presence of cerebral artery atherosclerosis and stroke subtypes. SUBJECTS/METHODS: A total of 147 patients and 144 control subjects were recruited consecutively in the metropolitan area of Seoul, Korea. Sixty participants each in the case and control groups were included in analyses after 1:1 frequency matching...
October 2016: Nutrition Research and Practice
Paola Donato, Veronica Inferrera, Danilo Sciarrone, Luigi Mondello
The task of lipid analysis has always challenged separation scientists, and new techniques in chromatography were often developed for the separation of lipids; however, no single technique or methodology is yet capable of affording a comprehensive screening of all lipid species and classes. This review acquaints the role of supercritical fluid chromatography within the field of lipid analysis, from the early developed capillary separations based on pure CO2 , to the most recent techniques employing packed columns under subcritical conditions, including the niche multidimensional techniques using supercritical fluids in at least one of the separation dimensions...
September 30, 2016: Journal of Separation Science
Lijun Xi, Nenghu Qiao, Zhenchong Zhang, Laihong Yan, Fengbo Li, Jinpeng Hu, Jing Li
A novel bacterium, strain L3(T), was isolated from an activated sludge sample retrieved from a municipal wastewater treatment plant in Huangdao, China. On the basis of 16S rRNA gene sequence similarity studies, strain L3(T) was affiliated to the genus Sinorhodobacter, being most closely related to Sinorhodobacter ferrireducens (98.0 %). The 16S rRNA gene sequence similarity of strain L3(T) to other related species, Thioclava atlantica DLFJ1-1(T) (96.5 %), Rhodobacter capsulatus ATCC 11166(T) (96.3 %), Paenirhodobacter enshiensis DW2-9(T) (96...
September 30, 2016: Antonie Van Leeuwenhoek
Jörn Lötsch, Alfred Ultsch
OBJECTIVE: The public accessibility of "big data" about the molecular targets of drugs and the biological functions of genes allows novel data science-based approaches to pharmacology that link drugs directly with their effects on pathophysiologic processes. This provides a phenotypic path to drug discovery and repurposing. This paper compares the performance of a functional genomics-based criterion to the traditional drug target-based classification. METHODS: Knowledge discovery in the DrugBank and Gene Ontology databases allowed the construction of a "drug target versus biological process" matrix as a combination of "drug versus genes" and "genes versus biological processes" matrices...
October 1, 2016: European Journal of Clinical Pharmacology
Richard J Albertini, Debra A Kaden
Formaldehyde (FA) is a mutagenic chemical - a property mitigated in vivo by rapid detoxification and limited tissue distribution following inhalation of the free agent. Endogenously produced FA is necessary for life and required for one-carbon transfer reactions; however, FA derived from external sources (exogenous FA), which may be in the form of methanol, may increase in vivo concentrations above naturally occurring physiological levels. Both endogenous and exogenous FA produce DNA monoadducts, DNA-DNA and DNA-protein cross-links (DDX and DPX) but, when exposed to exogenously-derived free FA, DNA monoadducts, DDX, and DPX are only produced at initial sites of contact...
August 11, 2016: Critical Reviews in Toxicology
Sophie Béliard, Aurélie Millier, Valérie Carreau, Alain Carrié, Philippe Moulin, Alexandre Fredenrich, Michel Farnier, Gérald Luc, David Rosenbaum, Mondher Toumi, Eric Bruckert
BACKGROUND: Heterozygous familial hypercholesterolemia (heFH) is a genetic disease causing high levels of low-density lipoprotein cholesterol (LDL-C). Although this population is at high cardiovascular (CV) risk, the risk is variable within patients depending on additional risk factors. CV disease risk groups have been defined by the Nouvelle Société Francophone d'Athérosclérose (NSFA) and by the National Lipid Association recommendations. OBJECTIVES: The study aimed to describe a sample of French heFH patients, comparing patients at very high risk (VHR) and patients at high risk in terms of demographic and clinical characteristics as well as biological measurements and disease management...
September 2016: Journal of Clinical Lipidology
Y Subhash, Min-Jeong Park, Sang-Seob Lee
A novel Gram-stain-negative, small curve-rod shaped, motile strain, designated L6T, was isolated from hydrocarbon contaminated soils collected from Kuwait. Strain L6T was able to grow at 10-40ºC (optimum, 27-32 ºC), pH 6.1-8.8 (optimum, 6.5-7.5) and 0-4.5% (w/v) NaCl (optimum, 0-0.5). C18:16c/C18:17c, C16:0, C16:16c/C16:17c, C12:0, C12:03-OH aldehyde were predominant fatty acids with minor amounts of C14:0 and C17:0 cyclo. Phosphatidylglycerol (PG) and phosphatidylethanolamine (PE) were major polar lipids...
September 21, 2016: International Journal of Systematic and Evolutionary Microbiology
Q D Plumlee, A M Hernandez, S D Clark, A Bascuñán, J Davidson, J Mansell
A 10-year-old, neutered male, Basset hound had a 26 × 21 × 21 cm infiltrative mass on the left abdominal wall that did not extend into the peritoneal cavity based on radiographs and abdominal computed tomography. Cytological examination revealed moderate numbers of neoplastic round cells, which frequently contained numerous round, clear, cytoplasmic vacuoles. Histologically, the tumour was composed of two morphologically distinct cell populations forming a continuum of heterogeneously differentiated cells...
September 21, 2016: Journal of Comparative Pathology
Mohammed N A Khalil, Mostafa I Fekry, Mohamed A Farag
Dates (Phoenix dactylifera L.) are distributed worldwide as major food complement providing a source of sugars and dietary fiber as well as macro- and micronutrients. Although phytochemical analyses of date fruit non-volatile metabolites have been reported, much less is known about the aroma given off by the fruit, which is critical for dissecting sensory properties and quality traits. Volatile constituents from 13 date varieties grown in Egypt were profiled using SPME-GCMS coupled to multivariate data analysis to explore date fruit aroma composition and investigate potential future uses by food industry...
February 15, 2017: Food Chemistry
Muneo Yamaguchi, Shintaro Nakao, Yoshihiro Kaizu, Yoshiyuki Kobayashi, Takahito Nakama, Mitsuru Arima, Shigeo Yoshida, Yuji Oshima, Atsunobu Takeda, Yasuhiro Ikeda, Shizuo Mukai, Tatsuro Ishibashi, Koh-Hei Sonoda
Histological studies from autopsy specimens have characterized hard exudates as a composition of lipid-laden macrophages or noncellular materials including lipid and proteinaceous substances (hyaline substances). However, the characteristics of hard exudates in living patients have not been examined due to insufficient resolution of existing equipment. In this study, we used adaptive optics scanning laser ophthalmoscopy (AO-SLO) to examine the characteristics of hard exudates in patients with retinal vascular diseases...
2016: Scientific Reports
Vanessa Giannetti, Maurizio Boccacci Mariani, Paola Mannino
An authentication study based on headspace solid-phase microextraction/GC-MS was performed with a set of 60 samples representative of traditional "Pasta di Gragnano protected geographical indication (PGI)" and the most common Italian pasta brands. Multivariate chemometric tools were used to classify the samples based on the chemical information provided from 20 target flavor compounds, including Maillard reaction and lipid oxidation products. Pattern recognition by principal component analysis and linear discriminant analysis showed a natural grouping of samples according to the drying process adopted for their production (i...
September 2016: Journal of AOAC International
Raşit Kιlιç, Aslhan Cavunt Bayraktar, Serdar Bayraktar, Ali Kurt, Mustafa Kavutçu
PURPOSE: The aim of this study was to evaluate the relationship between antioxidant superoxide dismutase (SOD) enzyme activity, malondialdehyde (MDA) as a lipid peroxidation marker, and some trace elements such as zinc (Zn) and copper (Cu) levels in patients with keratoconus. METHODS: A total of 58 patients with keratoconus and 53 control subjects with similar age and sex were evaluated in this study. The modified Krumeich keratoconus classification was used to divide the patients into 4 stages...
September 8, 2016: Cornea
Najla AlMasoud, Yun Xu, Drupad K Trivedi, Simona Salivo, Tom Abban, Nicholas J W Rattray, Ewa Szula, Haitham AlRabiah, Ali Sayqal, Royston Goodacre
Bacillus are aerobic spore-forming bacteria that are known to lead to specific diseases, such as anthrax and food poisoning. This study focuses on the characterization of these bacteria by the detection of lipids extracted from 33 well-characterized strains from the Bacillus and Brevibacillus genera, with the aim to discriminate between the different species. For the purpose of analysing the lipids extracted from these bacterial samples, two rapid physicochemical techniques were used: matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF-MS) and liquid chromatography in conjunction with mass spectrometry (LC-MS)...
November 2016: Analytical and Bioanalytical Chemistry
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"