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https://www.readbyqxmd.com/read/28214842/inhibition-of-methylglyoxal-induced-ages-rage-expression-contributes-to-dermal-protection-by-n-acetyl-l-cysteine
#1
Chun-Tao Yang, Fu-Hui Meng, Li Chen, Xiang Li, Lai-Jian Cen, Yu-Hua Wen, Cai-Chen Li, Hui Zhang
BACKGROUND/AIM: Accumulation of advanced glycation end products (AGEs) is a major cause of diabetes mellitus (DM) skin complications. Methylglyoxal (MGO), a reactive dicarbonyl compound, is a crucial intermediate of AGEs generation. N-acetyl-L-cysteine (NAC), an active ingredient of some medicines, can induce endogenous GSH and hydrogen sulfide generation, and set off a condensation reaction with MGO. However, there is rare evidence to show NAC can alleviate DM-induced skin injury through inhibition of AGEs generation or toxicity...
13, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28214706/influence-of-oxygen-partial-pressure-on-the-characteristics-of-human-hepatocarcinoma-cells
#2
Jenifer Trepiana, Susana Meijide, Rosaura Navarro, M Luisa Hernández, José Ignacio Ruiz-Sanz, M Begoña Ruiz-Larrea
Most of the in vitro studies using liver cell lines have been performed under atmospheric oxygen partial pressure (21% O2). However, the oxygen concentrations in the liver and cancer cells are far from this value. In the present study, we have evaluated the influence of oxygen on 1) the tumor cell lines features (growth, steady-state ROS levels, GSH content, activities of antioxidant enzymes, p66 Shc and SOD expressions, metalloproteinases secretion, migration, invasion, and adhesion) of human hepatocellular carcinoma cell lines, and b) the response of the cells to an oxidant stimulus (aqueous leaf extract of the V...
February 13, 2017: Redox Biology
https://www.readbyqxmd.com/read/28211947/insulin-like-growth-factor-1-promotes-osteogenic-differentiation-and-collagen-i-alpha-2-synthesis-via-induction-of-mrna-binding-protein-larp6-expression
#3
Yue Guo, Chen-Yi Tang, Xiao-Fei Man, Hao-Neng Tang, Jun Tang, Ci-La Zhou, Shu-Wen Tan, Min Wang, Yun-Zhi Feng, Hou-De Zhou
This study explored the mechanism underlying the stimulation of collagen synthesis and osteoblastic differentiation by insulin-like growth factor 1 (IGF1) in primary mouse osteoblasts. Primary mouse calvarial osteoblasts were cultured and treated with various doses of IGF1 before transfection with siRNA targeting the collagen type I alpha 2 (Col1a2) or La ribonucleoprotein domain family member 6 (Larp6) genes. Alkaline phosphatase (ALP) activity, osteocalcin staining, alizarin red quantification and the expression level of runt-related transcription factor 2 (RUNX2) were performed to assess the differentiation of pre-osteoblasts...
February 17, 2017: Development, Growth & Differentiation
https://www.readbyqxmd.com/read/28211531/proteomic-analysis-of-urinary-extracellular-vesicles-from-high-gleason-score-prostate-cancer
#4
Kazutoshi Fujita, Hideaki Kume, Kyosuke Matsuzaki, Atsunari Kawashima, Takeshi Ujike, Akira Nagahara, Motohide Uemura, Yasushi Miyagawa, Takeshi Tomonaga, Norio Nonomura
Extracellular vesicles (EVs) are microvesicles secreted from various cell types. We aimed to discover a new biomarker for high Gleason score (GS) prostate cancer (PCa) in urinary EVs via quantitative proteomics. EVs were isolated from urine after massage from 18 men (negative biopsy [n = 6], GS 6 PCa [n = 6], or GS 8-9 PCa [n = 6]). EV proteins were labeled with iTRAQ and analyzed by LC-MS/MS. We identified 4710 proteins and quantified 3528 proteins in the urinary EVs. Eleven proteins increased in patients with PCa compared to those with negative biopsy (ratio >1...
February 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28205039/a-pooled-analysis-of-clinical-pharmacology-trials-investigating-the-pharmacokinetic-and-pharmacodynamic-characteristics-of-fast-acting-insulin-aspart-in-adults-with-type-1-diabetes
#5
Tim Heise, Thomas R Pieber, Thomas Danne, Lars Erichsen, Hanne Haahr
BACKGROUND: Fast-acting insulin aspart (faster aspart) is insulin aspart (IAsp) in a new formulation aiming to mimic the fast endogenous prandial insulin release more closely than currently available insulin products. In a post hoc analysis of pooled data from six clinical pharmacology trials, the pharmacological characteristics of faster aspart and IAsp were compared. METHODS: The analysis included 218 adult subjects with type 1 diabetes from six randomised, double-blind, crossover trials in the faster aspart clinical development programme...
February 15, 2017: Clinical Pharmacokinetics
https://www.readbyqxmd.com/read/28204830/monoclonal-antibody-against-toll-like-receptor%C3%A2-4-attenuates-ventilator-induced-lung-injury-in-rats-by-inhibiting-myd88-and-nf-%C3%AE%C2%BAb-dependent-signaling
#6
Cuiyuan Huang, Linghui Pan, Fei Lin, Huijun Dai, Ruili Fu
The mechanisms through which mechanical ventilation causes non-infectious inflammatory diseases and lung injury are poorly understood. Animals models of this type of injury suggest that it involves signaling mediated by Toll‑like receptor (TLR)4 and 9. In this study, in order to gain further insight into the involvement of TLR4 in this type of injury, we performed in vivo and in vitro experiments to determine the mechanisms through which TLR4 triggers inflammation. We also examined whether the use of TLR4 monoclonal antibody (mAb) can alleviate this type of injury...
March 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28197374/tumor-derived-osteopontin-isoforms-cooperate-with-trp53-and-ccl2-to-promote-lung-metastasis
#7
Ioanna Giopanou, Ioannis Lilis, Vassilios Papaleonidopoulos, Theodora Agalioti, Nikolaos I Kanellakis, Nikolitsa Spiropoulou, Magda Spella, Georgios T Stathopoulos
The lungs are ubiquitous receptacles of metastases originating from various bodily tumors. Although osteopontin (SPP1) has been associated with tumor dissemination, the role of its isoforms in lung-directed metastasis is incompletely understood. We employed syngeneic mouse models of spontaneous and induced lung-targeted metastasis in C57BL/6 mice competent and deficient in both Spp1 alleles. Tumor-derived osteopontin expression was modulated using either stable anti-Spp1 RNA interference, or forced overexpression of intracellular and secreted Spp1 isoforms...
2017: Oncoimmunology
https://www.readbyqxmd.com/read/28196860/the-nclx-type-na-ca-2-exchanger-ncx-9-is-required-for-patterning-of-neural-circuits-in-caenorhabditis-elegans
#8
Vishal Sharma, Soumitra Roy, Israel Sekler, Damien M O'Halloran
NCLX is a Na(+)/Ca(2+) exchanger that uses energy stored in the transmembrane sodium gradient to facilitate the exchange of sodium ions for ionic calcium. Mammals have a single NCLX, which has been shown to function primarily at the mitochondrion and is an important regulator of neuronal physiology by contributing to neurotransmission and synaptic plasticity. The role of NCLX in developmental cell patterning, for example, in neural circuits, is largely unknown. Here we describe a novel role for the Caenorhabditis elegans NCLX-type protein, NCX-9, in neural circuit formation...
February 14, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28194325/serum-yb-1-y-box-binding-protein-1-as-a-biomarker-of-bone-disease-progression-in-patients-with-breast-cancer-and-bone-metastases
#9
A R Ferreira, M Bettencourt, I Alho, A L Costa, A R Sousa, A Mansinho, C Abreu, C Pulido, D Macedo, I Vendrell, T R Pacheco, L Costa, S Casimiro
YB-1 (Y-box binding protein 1) is a multifunctional cold-shock protein that has been implicated in all hallmarks of cancer. Elevated YB-1 protein level was associated with poor prognosis in several types of cancers, including breast cancer (BC), where it is a marker of decreased overall survival (OS) and distant metastasis-free survival across all subtypes. YB-1 is also secreted by different cell types and may act as an extracellular mitogen; however the pathological implications of the secreted form of YB-1 (sYB-1) are unknown...
March 2017: Journal of Bone Oncology
https://www.readbyqxmd.com/read/28185773/association-of-gene-polymorphisms-in-interleukin-6-in-infantile-bronchial-asthma
#10
Eva Babusikova, Jana Jurecekova, Milos Jesenak, Andrea Evinova
INTRODUCTION: The genetic background of bronchial asthma is complex, and it is likely that multiple genes contribute to its development both directly and through gene-gene interactions. Cytokines contribute to different aspects of asthma, as they determine the type, severity and outcomes of asthma pathogenesis. Allergic asthmatics undergoing an asthmatic attack exhibit significantly higher levels of pro-inflammatory cytokines, such as interleukins and chemokines. In recent years, cytokines and their receptors have been shown to be highly polymorphic, and this prompted us to investigate interleukin 6 promoter polymorphisms at position -174G/C (rs1800795) and at -572G/C (rs1800796) in relation to asthma in children...
February 6, 2017: Archivos de Bronconeumología
https://www.readbyqxmd.com/read/28177762/chronic-intermittent-hypoxia-disturbs-insulin-secretion-and-causes-pancreatic-injury-via-mapk-signaling-pathway
#11
Yeying Wang, Bing Hai, Xiaoqun Niu, Li Ai, Yu Cao, Ran Li, Yongxia Li
Obstructive sleep apnea (OSA) is a breath disorder during sleep with a most prominent character of chronic intermittent hypoxia (CIH), inducing the generation of reactive oxygen species (ROS) to damage multiple tissues and cause metabolism disorders. In this study, we established rat varying OSA model by graded concentration of CIH (12.5% O2, 10% O2, 7.5%O2 and 5% O2) for 12 weeks, and found that CIH stimulated insulin secretion, reduced the insulin/proinsulin ratio in the pancreatic tissue, and caused pancreatic tissue lesions and cells apoptosis in a dose-dependent manner...
November 28, 2016: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28174720/netrin-1-protects-hepatocytes-against-cell-death-through-sustained-translation-during-the-unfolded-protein-response
#12
Thomas Lahlali, Marie-Laure Plissonnier, Cristina Romero-López, Maud Michelet, Benjamin Ducarouge, Alfredo Berzal-Herranz, Fabien Zoulim, Patrick Mehlen, Romain Parent
BACKGROUND & AIMS: Netrin-1, a multifunctional secreted protein, is up-regulated in cancer and inflammation. Netrin-1 blocks apoptosis induced by the prototypical dependence receptors deleted in colorectal carcinoma and uncoordinated phenotype-5. Although the unfolded protein response (UPR) triggers apoptosis on exposure to stress, it first attempts to restore endoplasmic reticulum homeostasis to foster cell survival. Importantly, UPR is implicated in chronic liver conditions including hepatic oncogenesis...
May 2016: Cellular and Molecular Gastroenterology and Hepatology
https://www.readbyqxmd.com/read/28174562/deletion-of-the-chemokine-binding-protein-gene-from-the-parapoxvirus-orf-virus-reduces-virulence-and-pathogenesis-in-sheep
#13
Stephen B Fleming, Catherine McCaughan, Zabeen Lateef, Amy Dunn, Lyn M Wise, Nicola C Real, Andrew A Mercer
Orf virus (ORFV) is the type species of the Parapoxvirus genus of the family Poxviridae and infects sheep and goats, often around the mouth, resulting in acute pustular skin lesions. ORFV encodes several secreted immunomodulators including a broad-spectrum chemokine binding protein (CBP). Chemokines are a large family of secreted chemotactic proteins that activate and regulate inflammation induced leukocyte recruitment to sites of infection. In this study we investigated the role of CBP in vivo in the context of ORFV infection of sheep...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28167945/tlr7-and-tlr3-sense-brucella-abortus-rna-to-induce-proinflammatory-cytokine-production-but-they-are-dispensable-for-host-control-of-infection
#14
Priscila C Campos, Marco Túlio R Gomes, Erika S Guimarães, Gabriela Guimarães, Sergio C Oliveira
Brucella abortus is a Gram-negative, facultative intracellular bacterium that causes brucellosis, a worldwide zoonotic disease leading to undulant fever in humans and abortion in cattle. The immune response against this bacterium relies on the recognition of microbial pathogen-associated molecular patterns, such as lipoproteins, lipopolysaccharides, and DNA; however, the immunostimulatory potential of B. abortus RNA remains to be elucidated. Here, we show that dendritic cells (DCs) produce significant amounts of IL-12, IL-6, and IP-10/CXCL10, when stimulated with purified B...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28161850/potential-anti-proliferative-and-immunomodulatory-effects-of-marine-microalgal-exopolysaccharide-on-various-human-cancer-cells-and-lymphocytes-in-vitro
#15
Geon-Tae Park, Ryeo-Eun Go, Hae-Miru Lee, Geum-A Lee, Cho-Won Kim, Jeong-Woo Seo, Won-Kyung Hong, Kyung-Chul Choi, Kyung-A Hwang
Marine microalgal exopolysaccharides (EPSs) have drawn great attention due to their biotechnological potentials such as anti-viral, anti-oxidant, anti-lipidemic, anti-proliferative, and immunomodulatory activities, etc. In the present study, the EPS derived from microalgae Thraustochytriidae sp.-derived mutant GA was investigated for its anti-proliferation and immunomodulation. Anti-cancer efficacy of the microalgal EPS was examined for the alterations in cell proliferation and cell cycle-related gene expression that occur in three types of human cancer cell lines, BG-1 ovarian, MCF-7 breast, and SW-620 colon cancer cell lines, by its treatment...
February 4, 2017: Marine Biotechnology
https://www.readbyqxmd.com/read/28154457/evaluation-of-the-new-commercial-recombinant-chimeric-subunit-vaccine-prrsfree-in-challenge-with-heterologous-types-1-and-2-porcine-reproductive-and-respiratory-syndrome-virus
#16
Jiwoon Jeong, Changhoon Park, Kyuhyung Choi, Chanhee Chae
The objective of this study was to evaluate a new recombinant chimeric vaccine against porcine reproductive and respiratory syndrome virus (PRRSV). The subunit vaccine, PRRSFREE, from Reber Genetics, Taiwan, Republic of China, is based on a plasmid containing a detoxified Pseudomonas exotoxin carrying open reading frame (ORF) 7, 1b, and 5 and 6 chimeric subunits of types 1 and 2 PRRSV. Pigs were injected intramuscularly with 2.0 mL of the vaccine at 21 and 42 d of age, according to the manufacturer's recommendation...
January 2017: Canadian Journal of Veterinary Research, Revue Canadienne de Recherche Vétérinaire
https://www.readbyqxmd.com/read/28154372/therapeutic-effect-of-the-immunomodulatory-drug-lenalidomide-but-not-pomalidomide-in-experimental-models-of-rheumatoid-arthritis-and-inflammatory-bowel-disease
#17
Belen Lopez-Millan, Rafael Diaz de la Guardia, Heleia Roca-Ho, Carmen M García-Herrero, Jessie R Lavoie, Michael Rosu-Myles, Elena Gonzalez-Rey, Francisco O'Valle, Gabriel Criado, Mario Delgado, Pablo Menendez
Thalidomide is an immunomodulatory drug (IMiD) with proven therapeutic action in several autoimmune/inflammatory diseases; however, its inherent high toxicity has led to the development of more powerful and safer thalidomide analogs, including lenalidomide and pomalidomide. These are new generation IMiDs that exhibit direct antitumor activity as well as anti-inflammatory/immunomodulatory properties, and are FDA-approved for the treatment of several hematological malignances. Here we investigated the potential therapeutic effects of lenalidomide and pomalidomide in several experimental murine models of autoimmune/inflammatory diseases: 2,4,6-trinitrobenzene sulfonic acid- and dextran sulfate sodium-induced inflammatory bowel disease and type II collagen-induced arthritis...
February 3, 2017: Experimental & Molecular Medicine
https://www.readbyqxmd.com/read/28152394/dnaj-hsp40-of-streptococcus-pneumoniae-is-involved-in-bacterial-virulence-and-elicits-a-strong-natural-immune-reaction-via-pi3k-jnk
#18
Jin Cui, Chenyu Ma, Guo Ye, Yong Shi, Wenchun Xu, Liang Zhong, Jian Wang, Yibing Yin, Xuemei Zhang, Hong Wang
As a heat shock protein, DnaJ plays an important role in the pathogenesis of pneumococcal infection. However, how the virulence factor-DnaJ elicits host natural immunity still remains unclear. In this study, we investigated the effects of dnaJ deficiency in Streptococcus pneumoniae (S. pneumoniae) on bacterial virulence, and further explored the related molecular mechanisms in vivo and in vitro. By generating dnaJ deficient mutant (ΔdnaJ), the virulence and colonization were detected in murine pneumonia and sepsis models in vivo...
March 2017: Molecular Immunology
https://www.readbyqxmd.com/read/28149418/the-role-of-adrenomedullin-in-cardiovascular-response-to-exercise-a-review
#19
Krzysztof Krzeminski
Adrenomedullin (ADM), the product of the vascular endothelial and smooth muscle cells, and cardiomyocytes, is considered to be a local factor controlling vascular tone, cardiac contractility and renal sodium excretion. The aim of this article was to review the existing data on the effect of different types of exercise on plasma ADM concentration in healthy men. The results of studies on the effect of dynamic exercise on the plasma ADM are contradictory. Some authors reported an increase in plasma ADM, while others showed a slight decrease or did not observe any changes...
December 1, 2016: Journal of Human Kinetics
https://www.readbyqxmd.com/read/28148490/characterisation-of-osteophytes-as-an-autologous-bone-graft-source-an-experimental-study-in-vivo-and-in-vitro
#20
K Ishihara, K Okazaki, T Akiyama, Y Akasaki, Y Nakashima
OBJECTIVES: Osteophytes are products of active endochondral and intramembranous ossification, and therefore could theoretically provide significant efficacy as bone grafts. In this study, we compared the bone mineralisation effectiveness of osteophytes and cancellous bone, including their effects on secretion of growth factors and anabolic effects on osteoblasts. METHODS: Osteophytes and cancellous bone obtained from human patients were transplanted onto the calvaria of severe combined immunodeficient mice, with Calcein administered intra-peritoneally for fluorescent labelling of bone mineralisation...
February 2017: Bone & Joint Research
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