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https://www.readbyqxmd.com/read/29165597/gsta1-expression-is-correlated-with-aldosterone-level-in-kcnj5-mutated-adrenal-aldosterone-producing-adenoma
#1
Xintao Li, Baojun Wang, Lu Tang, Yu Zhang, Luyao Chen, Liangyou Gu, Fan Zhang, Jinzhi Ouyang, Xu Zhang
Context: KCNJ5 mutation is a major cause of aldosterone-producing adenoma (APA). The development of APA apart from KCNJ5 mutation is less investigated. Objective: To investigate other mechanisms affecting aldosterone secretion apart from KCNJ5. Patients and Methods: Six pairs of KCNJ5-mutated, high and low aldosterone-secreting APAs, five non-KCNJ5-mutated APAs and four normal adrenal glands were assayed by Affymetrix Genechip Human Transcriptome Array 2...
November 20, 2017: Journal of Clinical Endocrinology and Metabolism
https://www.readbyqxmd.com/read/29164422/efficient-production-and-secretion-of-oxaloacetate-from-halomonas-sp-km-1-under-aerobic-conditions
#2
Asuka Hannya, Taku Nishimura, Isao Matsushita, Jun Tsubota, Yoshikazu Kawata
The alkaliphilic, halophilic bacterium Halomonas sp. KM-1 can utilize glucose for the intracellular storage of the bioplastic poly-(R)-3-hydroxybutyric acid (PHB) and extracellular secretion of pyruvate under aerobic conditions. In this study, we investigated the effects of sodium chloride concentration on PHB accumulation and pyruvate secretion in the KM-1 strain and, unexpectedly, observed that oxaloacetate, an important intermediate chemical in the TCA cycle, glycogenesis, and aspartic acid biosynthesis, was secreted...
November 21, 2017: AMB Express
https://www.readbyqxmd.com/read/29164068/comparative-pan-genome-analysis-of-piscirickettsia-salmonis-reveals-genomic-divergences-within-genogroups
#3
Guillermo Nourdin-Galindo, Patricio Sánchez, Cristian F Molina, Daniela A Espinoza-Rojas, Cristian Oliver, Pamela Ruiz, Luis Vargas-Chacoff, Juan G Cárcamo, Jaime E Figueroa, Marcos Mancilla, Vinicius Maracaja-Coutinho, Alejandro J Yañez
Piscirickettsia salmonis is the etiological agent of salmonid rickettsial septicemia, a disease that seriously affects the salmonid industry. Despite efforts to genomically characterize P. salmonis, functional information on the life cycle, pathogenesis mechanisms, diagnosis, treatment, and control of this fish pathogen remain lacking. To address this knowledge gap, the present study conducted an in silico pan-genome analysis of 19 P. salmonis strains from distinct geographic locations and genogroups. Results revealed an expected open pan-genome of 3,463 genes and a core-genome of 1,732 genes...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/29163515/nanobody-based-delivery-systems-for-diagnosis-and-targeted-tumor-therapy
#4
REVIEW
Yaozhong Hu, Changxiao Liu, Serge Muyldermans
The development of innovative targeted therapeutic approaches are expected to surpass the efficacy of current forms of treatments and cause less damage to healthy cells surrounding the tumor site. Since the first development of targeting agents from hybridoma's, monoclonal antibodies (mAbs) have been employed to inhibit tumor growth and proliferation directly or to deliver effector molecules to tumor cells. However, the full potential of such a delivery strategy is hampered by the size of mAbs, which will obstruct the targeted delivery system to access the tumor tissue...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/29161089/protein-folding-and-the-challenges-of-maintaining-endoplasmic-reticulum-proteostasis-in-idiopathic-pulmonary-fibrosis
#5
Freddy Romero, Ross Summer
Alveolar epithelial type II (AEII) cells are "professional" secretory cells that synthesize and secrete massive quantities of proteins to produce pulmonary surfactant and maintain airway immune defenses. To facilitate this high level of protein synthesis, AEII cells are equipped with an elaborate endoplasmic reticulum (ER) structure and possess an abundance of the machinery needed to fold, assemble, and secrete proteins. However, conditions that suddenly increase the quantity of new proteins entering the ER or that impede the capacity of the ER to fold proteins can cause misfolded or unfolded proteins to accumulate in the ER lumen, also called ER stress...
November 2017: Annals of the American Thoracic Society
https://www.readbyqxmd.com/read/29160172/neural-differentiation-of-spheroids-derived-from-hipsc-msc-co-culture
#6
Liqing Song, Ang-Chen Tsai, Xuegang Yuan, Julie Bejoy, Sébastien Sart, Teng Ma, Yan Li
Organoids, the condensed 3-D tissues emerged at the early stage of organogenesis, are a promising approach to regenerate functional and vascularized organ mimics. While incorporation of heterotypic cell types such as human mesenchymal stem cells (hMSCs) and human induced pluripotent stem cells (hiPSCs) derived neural progenitors aid neural organ development, the interactions of secreted factors during neurogenesis have not been well understood. The objective of this study is to investigate the impact of the composition and structure of 3-D hybrid spheroids of hiPSCs and hMSCs on dorsal cortical differentiation and the secretion of extracellular matrices and trophic factors in vitro...
November 21, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29159853/the-use-of-cell-conditioned-medium-for-musculoskeletal-tissue-regeneration
#7
REVIEW
Francesca Veronesi, Veronica Borsari, Maria Sartori, Monia Orciani, Monica Mattioli-Belmonte Cima, Milena Fini
Tissue regenerative medicine combines the use of cells, scaffolds and molecules to repair damaged tissues. Different cell types are employed for musculoskeletal diseases, both differentiated and mesenchymal stromal cells (MSCs). In recent years, the hypothesis that cell-based therapy is guided principally by cell-secreted factors has become increasingly popular. The aim of the present literature review was to evaluate preclinical and clinical studies that used conditioned medium (CM), rich in cell-factors, for musculoskeletal regeneration with a cell-free approach...
November 20, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29157116/perinatal-exposure-to-high-dietary-advanced-glycation-end-products-in-transgenic-nod8-3-mice-leads-to-pancreatic-beta-cell-dysfunction
#8
Danielle J Borg, Felicia Y T Yap, Sahar Keshvari, David G Simmons, Linda A Gallo, Amelia K Fotheringham, Aowen Zhuang, Robyn M Slattery, Sumaira Z Hasnain, Melinda T Coughlan, Phillip Kantharidis, Josephine M Forbes
The contribution of environmental factors to pancreatic islet damage in type 1 diabetes remains poorly understood. In this study, we crossed mice susceptible to type 1 diabetes, where parental male (CD8(+) T cells specific for IGRP206-214; NOD8.3) and female (NOD/ShiLt) mice were randomized to a diet either low or high in AGE content and maintained on this diet throughout pregnancy and lactation. After weaning, NOD8.3(+) female offspring were identified and maintained on the same parental feeding regimen for until day 28 of life...
November 20, 2017: Islets
https://www.readbyqxmd.com/read/29156616/a-novel-mutation-in-the-fibrinogen-b%C3%AE-chain-c-490g-a-end-of-exon-3-causes-a-splicing-abnormality-and-ultimately-leads-to-congenital-hypofibrinogenemia
#9
Chiaki Taira, Kazuyuki Matsuda, Shinpei Arai, Mitsutoshi Sugano, Takeshi Uehara, Nobuo Okumura
We found a novel heterozygous mutation in the fibrinogen Bβ chain (c.490G>A) of a 3-year-old girl with congenital hypofibrinogenemia. To clarify the complex genetic mechanism, we made a mini-gene including a FGB c.490G>A mutation region, transfected it into a Chinese Hamster Ovary (CHO) cell line, and analyzed reverse transcription (RT) products. The assembly process and secretion were examined using recombinant mutant fibrinogen. Direct sequencing demonstrated that the mutant RT product was 99 bp longer than the wild-type product, and an extra 99 bases were derived from intron 3...
November 20, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29155326/genome-wide-identification-of-the-sweet-gene-family-in-wheat
#10
Yue Gao, Zi Yuan Wang, Vikranth Kumar, Xiao Feng Xu, De Peng Yuan, Xiao Feng Zhu, Tian Ya Li, Bao Lei Jia, Yuan Hu Xuan
The SWEET (sugars will eventually be exported transporter) family is a newly characterized group of sugar transporters. In plants, the key roles of SWEETs in phloem transport, nectar secretion, pollen nutrition, stress tolerance, and plant-pathogen interactions have been identified. SWEET family genes have been characterized in many plant species, but a comprehensive analysis of SWEET members has not yet been performed in wheat. Here, 59 wheat SWEETs (hereafter TaSWEETs) were identified through homology searches...
November 16, 2017: Gene
https://www.readbyqxmd.com/read/29155085/ir-spectroscopy-analysis-of-pancreatic-lipase-related-protein-2-interaction-with-phospholipids-2-discriminative-recognition-of-various-micellar-systems-and-characterization-of-plrp2-dppc-bile-salt-complexes
#11
Eduardo Mateos-Diaz, Priscila Sutto-Ortiz, Moulay Sahaka, Deborah Byrne, Hélène Gaussier, Frédéric Carrière
The interaction of pancreatic lipase-related protein 2 (PLRP2) with various micelles containing phospholipids was investigated using pHstat enzyme activity measurements, differential light scattering, size exclusion chromatography (SEC) and transmission IR spectroscopy. Various micelles of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and lysophosphatidylcholine were prepared with either bile salts (sodium taurodeoxycholate or glycodeoxycholate) or Triton X-100, which are substrate-dispersing agents commonly used for measuring phospholipase activities...
November 16, 2017: Chemistry and Physics of Lipids
https://www.readbyqxmd.com/read/29153777/vaccination-with-a-human-parainfluenza-virus-type-3-chimeric-fhn-glycoprotein-formulated-with-a-combination-adjuvant-induces-protective-immunity
#12
R Garg, R Brownlie, L Latimer, V Gerdts, A Potter, S van Drunen Littel-van den Hurk
Human parainfluenza virus type 3 (PIV3) is a major cause of lower respiratory disease i.e. bronchitis, bronchiolitis or pneumonia, in infants and young children. Presently there is no licensed vaccine against PIV3. To produce an effective subunit vaccine, a chimeric FHN glycoprotein consisting of the N-terminal ectodomain of the fusion (F) protein linked to the haemagglutinin-neuraminidase (HN) protein without transmembrane domain, and secreted forms of the individual F and HN glycoproteins, were expressed in mammalian cells and purified...
November 16, 2017: Vaccine
https://www.readbyqxmd.com/read/29151021/regulatory-t-cells-induced-by-b-cells-a-novel-subpopulation-of-regulatory-t-cells
#13
REVIEW
Chien-Hui Chien, Bor-Luen Chiang
Regulatory T cells play a crucial role in the homeostasis of the immune response. In addition to CD4(+)Foxp3(+) regulatory T cells, several subsets of Foxp3(-) regulatory T cells, such as T helper 3 (Th3) cells and type 1 regulatory T (Tr1) cells, have been described in mice and human. Accumulating evidence shows that naïve B cells contribute to tolerance and are able to promote regulatory T cell differentiation. Naïve B cells can convert CD4(+)CD25(-) T cells into CD25(+)Foxp3(-) regulatory T cells, named Treg-of-B cells by our group...
November 18, 2017: Journal of Biomedical Science
https://www.readbyqxmd.com/read/29147434/acute-radiation-induced-changes-in-sprague-dawley-rat-submandibular-glands-a-histomorphometric-analysis
#14
Manu Krishnan, Aatish Tennavan, Seema Saraswathy, Tarun Sekhri, Ajay Kumar Singh, Velu Nair
Background: Radiation-induced xerostomia is a distressing clinical condition that starts appearing from the initial stages of radiotherapy in head and neck cancer patients. Though submandibular glands contribute to maximum of the "resting salivary" secretions, most of the acute xerostomia experiments so far reported have been on animal parotid glands. Therefore, we assessed and quantified the histologic changes in submandibular glands of Sprague-Dawley (SD) rats using histomorphometry, 24 hours after radiation...
April 2017: World Journal of Oncology
https://www.readbyqxmd.com/read/29144809/preserved-proinsulin-secretion-in-long-standing-type-1-diabetes
#15
Devin W Steenkamp, Jose M Cacicedo, Ayse Sahin-Efe, Catherine Sullivan, Elliot Sternthal
OBJECTIVE: Recent literature, has reported preserved residual beta cell function, (c-peptide "microsecretion") in many individuals with long standing type 1 diabetes. However, the concentrations of detectable insulin/c-peptide in the serum are usually very low, and beta cell mass is typically negligible. Proinsulin is measurable in the early years after diagnosis, consistent with the presence of residual functioning beta cells. However, individuals are not expected to secrete significant amounts of proinsulin beyond the early years after diagnosis...
November 16, 2017: Endocrine Practice
https://www.readbyqxmd.com/read/29144750/investigation-of-the-physical-and-bioactive-properties-of-bromo-and-iodo-containing-sponge-derived-compounds-possessing-an-oxyphenylethanamine-core
#16
Erin P McCauley, Hanh Lam, Nicholas Lorig-Roach, Justin Luu, Cameron Lloyd, Karen Tenney, Halina Pietraszkiewicz, Cristina Diaz, Frederick A Valeriote, Victoria Auerbuch, Phillip Crews
This research set out to identify compounds from marine sponges that can act as bacterial virulence blockers. Extracts from a total of 80 sponges collected from throughout Indonesia were screened in a high-throughput NF-κB-based screen that identifies compounds capable of inhibiting the bacterial type III secretion system (T3SS) in Yersinia pseudotuberculosis. An extract that was shown to inhibit T3SS-driven NF-κB expression was obtained from an Iotrochota cf. iota sponge and was the source of seven new bromo- and iodo-containing compounds, all of which contain a 2-(4-oxyphenyl)ethan-1-amine core...
November 16, 2017: Journal of Natural Products
https://www.readbyqxmd.com/read/29144452/ubiquitination-and-degradation-of-gbps-by-a-shigella-effector-to-suppress-host-defence
#17
Peng Li, Wei Jiang, Qin Yu, Wang Liu, Ping Zhou, Jun Li, Junjie Xu, Bo Xu, Fengchao Wang, Feng Shao
Interferon-inducible guanylate-binding proteins (GBPs) mediate cell-autonomous antimicrobial defences. Shigella flexneri, a Gram-negative cytoplasmic free-living bacterium that causes bacillary dysentery, encodes a repertoire of highly similar type III secretion system effectors called invasion plasmid antigen Hs (IpaHs). IpaHs represent a large family of bacterial ubiquitin-ligases, but their function is poorly understood. Here we show that S. flexneri infection induces rapid proteasomal degradation of human guanylate binding protein-1 (hGBP1)...
November 16, 2017: Nature
https://www.readbyqxmd.com/read/29142230/unique-transcriptome-signatures-and-gm-csf-expression-in-lymphocytes-from-patients-with-spondyloarthritis
#18
M H Al-Mossawi, L Chen, H Fang, A Ridley, J de Wit, N Yager, A Hammitzsch, I Pulyakhina, B P Fairfax, D Simone, Yao Yi, S Bandyopadhyay, K Doig, R Gundle, B Kendrick, F Powrie, J C Knight, P Bowness
Spondyloarthritis encompasses a group of common inflammatory diseases thought to be driven by IL-17A-secreting type-17 lymphocytes. Here we show increased numbers of GM-CSF-producing CD4 and CD8 lymphocytes in the blood and joints of patients with spondyloarthritis, and increased numbers of IL-17A(+)GM-CSF(+) double-producing CD4, CD8, γδ and NK cells. GM-CSF production in CD4 T cells occurs both independently and in combination with classical Th1 and Th17 cytokines. Type 3 innate lymphoid cells producing predominantly GM-CSF are expanded in synovial tissues from patients with spondyloarthritis...
November 15, 2017: Nature Communications
https://www.readbyqxmd.com/read/29138867/effect-of-xanthohumol-on-th1-th2-balance-in-a-breast-cancer-mouse-model
#19
Wenchao Zhang, Yanlong Pan, Panhong Gou, Cheng Zhou, Lianqing Ma, Qiming Liu, Yuping Du, Jinbo Yang, Qin Wang
Xanthohumol (XN), a prenylflavonoid found in the hop plant, Humulus lupulus, exhibits a variety of biological activities. Numerous studies have reported that XN inhibits the growth of many types of cancer cells, but the effects of XN on tumor immunity have not yet been studied. We explored the effect of XN on Th1/Th2 balance and the underlying mechanism based on a BALB/c-4T1 breast cancer mouse model. The results showed that XN significantly slowed down tumor growth and inhibited expression of antitumor proliferation protein Ki-67 as well as breast cancer-specific marker cancer antigen 15-3 (CA15-3)...
November 13, 2017: Oncology Reports
https://www.readbyqxmd.com/read/29138800/broadleaf-mahonia-attenuates-granulomatous-lobular-mastitis%C3%A2-associated-inflammation-by-inhibiting-ccl%C3%A2-5-expression-in-macrophages
#20
Zhiyu Wang, Neng Wang, Xiaoyan Liu, Qi Wang, Biao Xu, Pengxi Liu, Huayu Zhu, Jianping Chen, Honglin Situ, Yi Lin
Granulomatous lobular mastitis (GLM) is a type of chronic mammary inflammation with unclear etiology. Currently systematic corticosteroids and methitrexate are considered as the main drugs for GLM treatment, but a high toxicity and risk of recurrence greatly limit their application. It is therefore an urgent requirement that safe and efficient natural drugs are found to improve the GLM prognosis. Broadleaf Mahonia (BM) is a traditional Chinese herb that is believed to have anti‑inflammatory properties according to ancient records of traditional Chinese medicine...
November 9, 2017: International Journal of Molecular Medicine
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