Stephan Wimmi, Moritz Fleck, Carlos Helbig, Corentin Brianceau, Katja Langenfeld, Witold G Szymanski, Georgia Angelidou, Timo Glatter, Andreas Diepold
In animal pathogens, assembly of the type III secretion system injectisome requires the presence of so-called pilotins, small lipoproteins that assist the formation of the secretin ring in the outer membrane. Using a combination of functional assays, interaction studies, proteomics, and live-cell microscopy, we determined the contribution of the pilotin to the assembly, function, and substrate selectivity of the T3SS and identified potential new downstream roles of pilotin proteins. In absence of its pilotin SctG, Yersinia enterocolitica forms few, largely polar injectisome sorting platforms and needles...
January 4, 2024: Molecular Microbiology