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https://www.readbyqxmd.com/read/29786938/inhibition-and-kinetic-studies-of-lignin-degrading-enzymes-of-ganoderma-boninense-by-naturally-occurring-phenolic-compounds
#1
Arthy Surendran, Yasmeen Siddiqui, Halimi Mohd Saud, Nusaibah Syd Ali, Sivakumar Manickam
AIM: Lignolytic (Lignin degrading) enzyme, from oil palm pathogen Ganoderma boninense Pat. (Syn G. orbiforme (Ryvarden), is involved in the detoxification and the degradation of lignin in the oil palm and is the rate-limiting step in the infection process of this fungus. Active inhibition of lignin degrading enzymes secreted by G. boninense by various naturally occurring phenolic compounds and estimation of efficiency on pathogen suppression was aimed at. METHODS AND RESULTS: In our work, ten naturally occurring phenolic compounds were evaluated for their inhibitory potential towards the lignolytic enzymes of G...
May 22, 2018: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29786795/protocols-for-studies-on-stromal-cells-in-prostate-cancer
#2
Damien A Leach, Grant Buchanan
Interactions between tumor cells and fibroblasts play a pivotal role in cancer development and progression. Indeed, the paracrine communication between these two cell types is known to have physiological effects that alter carcinogenic and metastatic potential. An often overlooked player in these interactions is the involvement of the extracellular matrix (ECM). The network of ECM proteins secreted from fibroblasts is reportedly altered with cancer initiation and progression, and in several cases has been associated with patient outcome...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29786687/genome-wide-analysis-of-circular-rnas-in-bovine-cumulus-cells-treated-with-bmp15-and-gdf9
#3
Yao Fu, Hao Jiang, Jian-Bo Liu, Xu-Lei Sun, Zhe Zhang, Sheng Li, Yan Gao, Bao Yuan, Jia-Bao Zhang
Circular RNAs (circRNAs) are important members of the non-coding RNA family, and those relating to animal physiologies have been widely studied in recent years. This study aimed to explore the roles of circRNAs in the regulation of follicular development. We constructed four bovine cumulus cell cDNA libraries, including a negative control group (NC) and groups treated with BMP15, GDF9 and BMP15 + GDF9, and we sequenced the libraries on the Illumina HiSeq Xten platform. We identified 1706 circRNAs and screened for differential circRNA expression...
May 21, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29786602/microfluidic-system-for-modelling-3d-tumour-invasion-into-surrounding-stroma-and-drug-screening
#4
Zhichang Du, Shengli Mi, Xiaoman Yi, Yuanyuan Xu, Wei Sun
Tumour invasion into the surrounding stroma is a critical step in metastasis, and it is necessary to clarify the role of microenvironmental factors in tumour invasion. We present a microfluidic system that simulated and controlled multi-factors of the tumour microenvironment for three-dimensional (3D) assessment of tumour invasion into the stroma. The simultaneous, precise and continuous arrangement of two 3D matrices was visualised to observe the migration of cancer cell populations or single cells by transfecting cells with a fluorescent protein...
May 22, 2018: Biofabrication
https://www.readbyqxmd.com/read/29786517/estrogen-receptor-%C3%AE-potential-target-for-therapy-in-adult-granulosa-cell-tumors
#5
Alessandra Ciucci, Gabriella Ferrandina, Floriana Mascilini, Flavia Filippetti, Giovanni Scambia, Gian Franco Zannoni, Daniela Gallo
OBJECTIVE: Adult granulosa cell tumor (AGCT) is a rare form of sex-cord stromal ovarian tumors. Due to their origin, AGCTs secrete estrogens, and thus, estrogen receptor (ER)-mediated signaling has been considered as a possible target for therapy. The aim of the present study was to get insights into estrogen receptor status and activity in AGCTs, as a strategy to provide molecular support for personalized hormonal treatments. METHODS: We evaluated by immunohistochemistry the expression of ERα, ERβ isoforms (i...
May 18, 2018: Gynecologic Oncology
https://www.readbyqxmd.com/read/29786111/high-glucose-suppresses-the-viability-and-proliferation-of-htr%C3%A2-8-svneo-cells-through-regulation-of-the-mir%C3%A2-137-prkaa1-il%C3%A2-6-axis
#6
Hai-Yan Peng, Ming-Qing Li, Hua-Ping Li
The aim of the present study was to investigate the mechanism underlying the high glucose (HG)‑associated regulation of HTR‑8/SVneo cell viability and proliferation during gestational diabetes mellitus (GDM), and to verify the association of microRNA (miR)‑137, protein kinase AMP‑activated catalytic subunit α1 (PRKAA1) and interlukin‑6 (IL‑6). miR‑137‑overexpressing and negative control HTR‑8/SVneo cells were established by lentiviral vector infection. Cell Counting Kit‑8 and colony formation assays were used to analyze the viability and proliferation of HTR‑8/SVneo cells...
May 17, 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29785766/schizophyllum-commune-an-unexploited-source-for-lignocellulose-degrading-enzymes
#7
REVIEW
Omar Eduardo Tovar-Herrera, Adriana Mayrel Martha-Paz, Yordanis Pérez-LLano, Elisabet Aranda, Juan Enrique Tacoronte-Morales, María Teresa Pedroso-Cabrera, Katiushka Arévalo-Niño, Jorge Luis Folch-Mallol, Ramón Alberto Batista-García
Lignocellulose represents the most abundant source of carbon in the Earth. Thus, fraction technology of the biomass turns up as an emerging technology for the development of biorefineries. Saccharification and fermentation processes require the formulation of enzymatic cocktails or the development of microorganisms (naturally or genetically modified) with the appropriate toolbox to produce a cost-effective fermentation technology. Therefore, the search for microorganisms capable of developing effective cellulose hydrolysis represents one of the main challenges in this era...
May 21, 2018: MicrobiologyOpen
https://www.readbyqxmd.com/read/29785668/hac1p-homologues-from-higher-eukaryotes-can-improve-the-secretion-of-heterologous-proteins-in-the-yeast-pichia-pastoris
#8
Olufemi Emmanuel Bankefa, Meiyu Wang, Taicheng Zhu, Yin Li
OBJECTIVES: To systematically explore the effects of overexpressing Hac1p homologues from different sources on protein secretion in Pichia pastoris system. RESULTS: Effects of Hac1p homologues encompassing P. pastoris (PpHac1p), S. cerevisiae (ScHac1p), Trichoderma reesei (TrHac1p) and Homo sapiens (HsXbp1), on secretion of three reporter proteins-β-galactosidase, β-mannanase and glucose oxidase were investigated. No individual Hac1p was optimal for all the enzymes...
May 21, 2018: Biotechnology Letters
https://www.readbyqxmd.com/read/29785587/a-study-on-the-use-of-strain-specific-and-homologous-promoters-for-heterologous-expression-in-industrial-saccharomyces-cerevisiae-strains
#9
Daniel Pereira de Paiva, Tiago Benoliel Rocha, Marciano Regis Rubini, André Moraes Nicola, Viviane Castelo Branco Reis, Fernando Araripe Gonçalves Torres, Lidia Maria Pepe de Moraes
Polymorphism is well known in Saccharomyces cerevisiae strains used for different industrial applications, however little is known about its effects on promoter efficiency. In order to test this, five different promoters derived from an industrial and a laboratory (S288c) strain were used to drive the expression of eGFP reporter gene in both cells. The ADH1 promoter (P ADH1 ) in particular, which showed more polymorphism among the promoters analyzed, also exhibited the highest differences in intracellular fluorescence production...
May 21, 2018: AMB Express
https://www.readbyqxmd.com/read/29785473/changes-in-melatonin-cortisol-and-body-temperature-and-the-relationship-between-endogenous-melatonin-levels-and-analgesia-consumption-in-patients-undergoing-bariatric-surgery
#10
Neslihan Altunkaya, Mehmet Ali Erdogan, Ulku Ozgul, Mukadder Sanli, Muharrem Ucar, Onural Ozhan, Fatih Sumer, Selim Erdogan, Cemil Colak, Mahmut Durmus
BACKGROUND: Melatonin has analgesic, anti-inflammatory, sedative, and anxiolytic properties. However, the relationship between endogenous melatonin levels and postoperative analgesic requirements has not been well elucidated in patients undergoing bariatric surgery. We studied endogenous melatonin levels, cortisol levels, body temperatures, and the relationship between the level of endogenous melatonin and postoperative morphine consumption. METHODS: The trial was conducted among 30 patients who were scheduled for laparoscopic bariatric surgery...
May 21, 2018: Obesity Surgery
https://www.readbyqxmd.com/read/29785472/the-impacts-of-gastroileostomy-rat-model-on-glucagon-like-peptide-1-a-promising-model-to-control-type-2-diabetes-mellitus
#11
Behrouz Keleidari, Rastin Mohammadi Mofrad, Shahab Shahabi Shahmiri, Mohammad Hossein Sanei, Mohsen Kolahdouzan, Erfan Sheikhbahaei
BACKGROUND: One of the new current treatment options for Diabetes Mellitus is about increasing glucagon-like peptide-1 (GLP-1) activity. GLP-1 with its incretin effect showed major role in glucose homeostasis. Gastroileostomy can increase GLP-1 secretion by rapid delivery of undigested food to the terminal ileum. We studied the early effects of a gastroileostomy on serum levels of GLP-1, glucose, and insulin in rats. METHODS: Gastroileostomies with side-to-side anastomosis were performed on 15 male New Zealand rats...
May 21, 2018: Obesity Surgery
https://www.readbyqxmd.com/read/29785210/adipose-tissue-derived-cytokines-and-their-correlations-with-clinical-characteristics-in-vietnamese-patients-with-type-2-diabetes-mellitus
#12
Nguyen Linh Toan, Nguyen Van Hoan, Doan Viet Cuong, Nguyen Viet Dung, Phan The Dung, Ngo Thu Hang, Do Thi Huyen Dieu, Dang Thanh Chung, Ho Anh Son, Pham Xuan Phong, George Binh Lenon, Doan Van De, Hoang Van Tong
Background: Adipokines are involved in the pathogenesis of metabolic disorders including obesity and type 2 diabetes mellitus (T2DM). This study investigates the levels of leptin, resistin, visfatin, secreted frizzled-related protein 5 (SFRP5), monocyte chemoattractant protein-1 (MCP-1) and retinol-binding protein 4 (RBP4) and their correlations with clinical parameters of overweight and T2DM. Methods: We recruited overweight 50 patients with T2DM, 88 non-overweight patients with T2DM, 29 overweight and 100 non-overweight individuals devoid of T2DM for this study...
2018: Diabetology & Metabolic Syndrome
https://www.readbyqxmd.com/read/29785114/convenient-food-made-of-extruded-adzuki-bean-attenuates-inflammation-and-improves-glycemic-control-in-patients-with-type-2-diabetes-a-randomized-controlled-trial
#13
Yanping Liu, Qiyan Wang, Shanshan Li, Yanfen Yue, Yuling Ma, Guixing Ren
Objective: Extrusion is a widely used food processing technology. The aim of this study was to investigate the effects of extruded adzuki bean convenient food (EABCF) on glycemic and inflammation control in type 2 diabetes mellitus (T2DM) patients. Patients and methods: In a randomized controlled trial, 120 T2DM patients were randomly assigned to a control diet group (the low glycemic index [LGI] group, assigned the traditional diabetic low glycemic index diet) or an intervention group (the EABCF group, assigned daily consumption of EABCF)...
2018: Therapeutics and Clinical Risk Management
https://www.readbyqxmd.com/read/29785015/frequent-transmission-of-the-mycobacterium-tuberculosis-beijing-lineage-and-positive-selection-for-the-esxw-beijing-variant-in-vietnam
#14
Kathryn E Holt, Paul McAdam, Phan Vuong Khac Thai, Nguyen Thuy Thuong Thuong, Dang Thi Minh Ha, Nguyen Ngoc Lan, Nguyen Huu Lan, Nguyen Thi Quynh Nhu, Hoang Thanh Hai, Vu Thi Ngoc Ha, Guy Thwaites, David J Edwards, Artika P Nath, Kym Pham, David B Ascher, Jeremy Farrar, Chiea Chuen Khor, Yik Ying Teo, Michael Inouye, Maxine Caws, Sarah J Dunstan
To examine the transmission dynamics of Mycobacterium tuberculosis (Mtb) isolated from tuberculosis patients in Ho Chi Minh City, Vietnam, we sequenced the whole genomes of 1,635 isolates and compared these with 3,144 isolates from elsewhere. The data identify an underlying burden of disease caused by the endemic Mtb lineage 1 associated with the activation of long-term latent infection, and a threefold higher burden associated with the more recently introduced Beijing lineage and lineage 4 Mtb strains. We find that Beijing lineage Mtb is frequently transferred between Vietnam and other countries, and detect higher levels of transmission of Beijing lineage strains within this host population than the endemic lineage 1 Mtb...
May 21, 2018: Nature Genetics
https://www.readbyqxmd.com/read/29784975/a-unique-cytoplasmic-atpase-complex-defines-the-legionella-pneumophila-type-iv-secretion-channel
#15
David Chetrit, Bo Hu, Peter J Christie, Craig R Roy, Jun Liu
Type IV secretion systems (T4SSs) are complex machines used by bacteria to deliver protein and DNA complexes into target host cells1-5 . Conserved ATPases are essential for T4SS function, but how they coordinate their activities to promote substrate transfer remains poorly understood. Here, we show that the DotB ATPase associates with the Dot-Icm T4SS at the Legionella cell pole through interactions with the DotO ATPase. The structure of the Dot-Icm apparatus was solved in situ by cryo-electron tomography at 3...
May 21, 2018: Nature Microbiology
https://www.readbyqxmd.com/read/29784969/north-american-domestic-pigs-are-susceptible-to-experimental-infection-with-japanese-encephalitis-virus
#16
So Lee Park, Yan-Jang S Huang, Amy C Lyons, Victoria B Ayers, Susan M Hettenbach, D Scott McVey, Kenneth R Burton, Stephen Higgs, Dana L Vanlandingham
Japanese encephalitis virus (JEV) is a mosquito-borne flavivirus that is capable of causing encephalitic diseases in children. While humans can succumb to severe disease, the transmission cycle is maintained by viremic birds and pigs in endemic regions. Although JEV is regarded as a significant threat to the United States (U.S.), the susceptibility of domestic swine to JEV infection has not been evaluated. In this study, domestic pigs from North America were intravenously challenged with JEV to characterize the pathological outcomes...
May 21, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29784887/identification-of-the-thioredoxin-partner-of-vkor-in-mycobacterial-disulfide-bond-formation
#17
Na Ke, Cristina Landeta, Xiaoyun Wang, Dana Boyd, Markus Eser, Jon Beckwith
Disulfide bonds influence stability and activity of many proteins. In Escherichia coli the DsbA and DsbB enzymes promote disulfide bond formation. Other bacteria, including the Actinobacteria, use instead of DsbB the enzyme VKOR (Vitamin K Epoxide Reductase), which is found either fused to or encoded in the same operon as a dsbA -like protein. Mycobacterium tuberculosis and other gram-positive Actinobacteria secrete to the cell envelope many proteins with even numbers of cysteines. These organisms have predicted oxidoreductases and VKOR orthologs...
May 21, 2018: Journal of Bacteriology
https://www.readbyqxmd.com/read/29784884/the-effector-gene-xopae-of-xanthomonas-euvesicatoria-85-10-is-part-of-an-operon-and-encodes-an-e3-ubiquitin-ligase
#18
Georgy Popov, Bharat Bhusan Majhi, Guido Sessa
The type III effector XopAE from the Xanthomonas euvesicatoria strain 85-10 ( Xe 85-10) was previously shown to inhibit plant immunity and enhance pathogen-induced disease symptoms. Evolutionary analysis of 60 xopAE alleles ( AEal ) revealed that the xopAE locus is conserved in multiple Xanthomonas species. The majority of xopAE alleles (55 out of 60) encodes a single ORF ( xopAE ), while in 5 alleles, including AEal 37 of the Xe 85-10 strain, a frame-shift splits the locus into two ORFs ( hpaF and a truncated xopAE )...
May 21, 2018: Journal of Bacteriology
https://www.readbyqxmd.com/read/29784872/sirt1-inhibits-rheumatoid-arthritis-fibroblast-like-synoviocyte-aggressiveness-and-inflammatory-response-via-suppressing-nf-%C3%AE%C2%BAb-pathway
#19
Guoqing Li, Zhongbing Xia, Ying Liu, Fanru Meng, Xia Wu, Yuxuan Fang, Chunwang Zhang, Dan Liu
Rheumatoid arthritis (RA) is an autoimmune disease of the joints characterized by synovial hyperplasia and chronic inflammation. Fibroblast-like synoviocytes (FLS) play a central role in RA initiation, progression, and perpetuation. Prior studies showed that sirtuin 1(SIRT1), a deacetylase participating in a broad range of transcriptional and metabolic regulations, may impact cell proliferation and inflammatory responses. However, the role of SIRT1 in RA-FLS was unclear. Here, we explored the effects of SIRT1 on the aggressiveness and inflammatory responses of cultured RA-FLS...
May 21, 2018: Bioscience Reports
https://www.readbyqxmd.com/read/29784864/group-iia-secreted-phospholipase-a-2-in-human-serum-kills-commensal-but-not-clinical-enterococcus-faecium-isolates
#20
Fernanda L Paganelli, Helen L Leavis, Samantha He, Nina M van Sorge, Christine Payré, Gérard Lambeau, Rob J L Willems, Suzan H M Rooijakkers
Human innate immunity employs cellular and humoral mechanisms to facilitate rapid killing of invading bacteria. The direct killing of bacteria by human serum is mainly attributed to the activity of the complement system that forms pores in Gram-negative bacteria. Although Gram-positive bacteria are considered resistant to serum killing, we here uncover that normal human serum effectively kills Enterococcus faecium. Comparison of a well-characterized collection of commensal and clinical E. faecium isolates revealed that human serum specifically kills commensal E...
May 21, 2018: Infection and Immunity
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