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https://www.readbyqxmd.com/read/28231157/breads-fortified-with-freeze-dried-vegetables-quality-and-nutritional-attributes-part-ii-breads-not-containing-oil-as-an-ingredient
#1
Viren Ranawana, Fiona Campbell, Charles Bestwick, Phyllis Nicol, Lesley Milne, Garry Duthie, Vassilios Raikos
The present article describes the second part of a study investigating the effect of adding vegetables on the nutritional, physico-chemical, and oxidative properties of wheat bread, and specifically focuses on bread that does not contain oil as an added ingredient. Wheat flour breads fortified with freeze-dried carrot, tomato, beetroot or broccoli were developed and assessed for their nutritional composition, antioxidant potential, oxidative stability, and storage properties. Using a simulated in vitro model, the study also examined the impact of vegetable addition on the oxidative stability of macronutrients during gastro-intestinal digestion...
September 8, 2016: Foods (Basel, Switzerland)
https://www.readbyqxmd.com/read/28231114/breads-fortified-with-freeze-dried-vegetables-quality-and-nutritional-attributes-part-1-breads-containing-oil-as-an-ingredient
#2
Viren Ranawana, Vassilios Raikos, Fiona Campbell, Charles Bestwick, Phyllis Nicol, Lesley Milne, Garry Duthie
There is increasing emphasis on reformulating processed foods to make them healthier. This study for the first time comprehensively investigated the effects of fortifying bread (containing oil as an ingredient) with freeze-dried vegetables on its nutritional and physico-chemical attributes. Breads fortified with carrot, tomato, beetroot or broccoli were assessed for nutrition, antioxidant potential, storage life, shelf stability, textural changes and macronutrient oxidation. Furthermore, using an in vitro model the study for the first time examined the impact of vegetable addition on the oxidative stability of macronutrients during human gastro-intestinal digestion...
March 14, 2016: Foods (Basel, Switzerland)
https://www.readbyqxmd.com/read/28230766/extraction-and-identification-of-phlorotannins-from-the-brown-alga-sargassum-fusiforme-harvey-setchell
#3
Yajing Li, Xiaoting Fu, Delin Duan, Xiaoyong Liu, Jiachao Xu, Xin Gao
Phlorotannins are a group of complex polymers of phloroglucinol (1,3,5-trihydroxybenzene), which are unique compounds from marine brown algae. In our present study, a procedure for extraction and enrichment of phlorotannins from S. fusiforme with highly antioxidant potentials was established. After comparison of different extraction methods, the optimal extraction conditions were established as follows. The freeze-dried seaweed powder was extracted with 30% ethanol-water solvent with a solid/liquid ratio of 1:5 at temperature of 25 °C for 30 min...
February 21, 2017: Marine Drugs
https://www.readbyqxmd.com/read/28230349/exceptional-anti-icing-performance-of-self-impregnating-slippery-surfaces
#4
Christos Stamatopoulos, Jaroslav Hemrle, Danhong Wang, Dimos Poulikakos
A heat exchange interface at subzero temperature in a water vapor environment, exhibits high probability of frost formation due to freezing condensation, a factor that markedly decreases the heat transfer efficacy due to the considerable thermal resistance of ice. Here we report a novel strategy to delay ice nucleation on these types of solid-water vapor interfaces. With a process-driven mechanism, a self-generated liquid intervening layer immiscible to water, is deposited on a textured superhydrophobic surface and acts as a barrier between the water vapor and the solid substrate...
February 23, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28229421/methods-for-long-term-storage-of-murine-bone-marrow-derived-mesenchymal-stem-cells
#5
Niketa Sareen, Ejlal Abu-El-Rub, Glen Lester Sequiera, Meenal Moudgil, Sanjiv Dhingra
This chapter is based on a simplified method to validate the current preservation procedure of mesenchymal stem cells (MSCs). Currently, there are various media available for freezing and thus preserving the MSCs, making it hard to decide which agent will be apt for cellular requirements. The study describes the effect of two different compositions of freezing media used in regular cell culture experiments, on the morphology, proliferation, and doubling rate of MSCs. Commonly used agents for the cryopreservation of MSCs include DMSO (Dimethyl Sulfoxide) and FBS (Fetal Bovine Serum) and DMEM (Dulbecco's Modified Eagle Medium)...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28228962/conforming-with-current-regulation-in-turkey-regarding-the-freezing-of-oocytes-a-case-report-of-the-first-pregnancy-in-turkey-achieved-through-oocyte-vitrification
#6
Semra Kahraman, Kahraman Ulker, Caroline Pirkevi Çetinkaya, Yesim Kumtepe Colakoglu, Hakan K Yelke, Cihangir Yilanlioglu
OBJECTIVES: To present the first pregnancy achieved in Turkey with frozen-warmed oocytes in a case with previous nine unsuccessful assisted reproductive technology (ART) attempts. METHODS: The clinical follow-up of a 33-year-old female applying to our ART centre after a long and complicated history of infertility is described. RESULTS: In April 2013, the woman attempted our centre for her 10th ART trial. She informed us on oocyte pick-up (OPU) day that her husband had been hospitalized following a car crush in Albania and was unable to travel to our clinic to give a sperm sample...
2017: SAGE open medical case reports
https://www.readbyqxmd.com/read/28228859/dynamics-and-interactions-of-ibuprofen-in-cyclodextrin-nanosponges-by-solid-state-nmr-spectroscopy
#7
Monica Ferro, Franca Castiglione, Nadia Pastori, Carlo Punta, Lucio Melone, Walter Panzeri, Barbara Rossi, Francesco Trotta, Andrea Mele
Two different formulations of cyclodextrin nanosponges (CDNS), obtained by polycondensation of β-cyclodextrin with ethylenediaminetetraacetic acid dianhydride (EDTAn), were treated with aqueous solutions of ibuprofen sodium salt (IbuNa) affording hydrogels that, after lyophilisation, gave two solid CDNS-drug formulations. (1)H fast MAS NMR and (13)C CP-MAS NMR spectra showed that IbuNa was converted in situ into its acidic and dimeric form (IbuH) after freeze-drying. (13)C CP-MAS NMR spectra also indicated that the structure of the nanosponge did not undergo changes upon drug loading compared to the unloaded system...
2017: Beilstein Journal of Organic Chemistry
https://www.readbyqxmd.com/read/28228570/urinary-p75-ecd-a-prognostic-disease-progression-and-pharmacodynamic-biomarker-in-als
#8
Stephanie R Shepheard, Joanne Wuu, Michell Cardoso, Luke Wiklendt, Phil G Dinning, Tim Chataway, David Schultz, Michael Benatar, Mary-Louise Rogers
OBJECTIVE: To evaluate urinary neurotrophin receptor p75 extracellular domain (p75(ECD)) levels as disease progression and prognostic biomarkers in amyotrophic lateral sclerosis (ALS). METHODS: The population in this study comprised 45 healthy controls and 54 people with ALS, 31 of whom were sampled longitudinally. Urinary p75(ECD) was measured using an enzyme-linked immunoassay and validation included intra-assay and inter-assay coefficients of variation, effect of circadian rhythm, and stability over time at room temperature, 4°C, and repeated freeze-thaw cycles...
February 22, 2017: Neurology
https://www.readbyqxmd.com/read/28228353/isochoric-and-isobaric-freezing-of-fish-muscle
#9
Gabriel Năstase, Chenang Lyu, Gideon Ukpai, Alexandru Șerban, Boris Rubinsky
We have recently shown that, a living organism, which succumbs to freezing to -4 °C in an isobaric thermodynamic system (constant atmospheric pressure), can survive freezing to -4 °C in an isochoric thermodynamic system (constant volume). It is known that the mechanism of cell damage in an isobaric system is the freezing caused increase in extracellular osmolality, and, the consequent cell dehydration. An explanation for the observed survival during isochoric freezing is the thermodynamic modeling supported hypothesis that, in the isochoric frozen solution the extracellular osmolality is comparable to the cell intracellular osmolality...
February 19, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28227901/identifying-montages-that-best-detect-the-electroencephalogram-power-spectrum-alteration-during-freezing-of-gait-in-parkinson-s-disease-patients
#10
Quynh Tran Ly, A M Ardi Handojoseno, Moran Gilat, Nghia Nguyen, Rifai Chai, Yvonne Tran, Simon J G Lewis, Hung T Nguyen, Quynh Tran Ly, A M Ardi Handojoseno, Moran Gilat, Nghia Nguyen, Rifai Chai, Yvonne Tran, Simon J G Lewis, Hung T Nguyen, Nghia Nguyen, A M Ardi Handojoseno, Moran Gilat, Hung T Nguyen, Yvonne Tran, Simon J G Lewis, Quynh Tran Ly, Rifai Chai
Our research team has previously used four Electroencephalography (EEG) leads to successfully detect and predict Freezing of Gait (FOG) in Parkinson's disease (PD). However, it remained to be determined whether these four sensor locations that were arbitrarily chosen based on their role in motor control are indeed the most optimal for FOG detection. The aim of this study was therefore to determine the most optimal location and combination of sensors to detect FOG amongst a 32-channel EEG montage using our EEG classification system...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28227838/alleviating-freezing-of-gait-using-phase-dependent-tactile-biofeedback
#11
Will Harrington, Andrew Greenberg, Edward King, James McNames, Lars Holmstrom, Fay B Horak, Martina Mancini, Will Harrington, Andrew Greenberg, Edward King, James McNames, Lars Holmstrom, Fay B Horak, Martina Mancini, Edward King, Lars Holmstrom, Martina Mancini, James McNames, Fay B Horak, Will Harrington, Andrew Greenberg
In this feasibility study, we present a novel, wearable prototype of tactile biofeedback to alleviate gait disturbances, such as freezing of gait in Parkinson's disease. We designed and tested a phase-dependent tactile biofeedback system that can be easily worn on the feet, with a simple switch to turn it on or off. Preliminary validation was performed in 8 subjects with Parkinson's disease who show freezing during a turning in place test. A metronome, control condition was used to compare effectiveness in alleviating freezing...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28226636/non-invasive-detection-of-the-freezing-of-gait-in-parkinson-s-disease-using-spectral-and-wavelet-features
#12
Kimia Nazarzadeh, Sridhar P Arjunan, Dinesh K Kumar, Debi Prasad Das, Kimia Nazarzadeh, Sridhar P Arjunan, Dinesh K Kumar, Debi Prasad Das, Debi Prasad Das, Dinesh K Kumar, Kimia Nazarzadeh, Sridhar P Arjunan
In this study, we have analyzed the accelerometer data recorded during gait analysis of Parkinson disease patients for detecting freezing of gait (FOG) episodes. The proposed method filters the recordings for noise reduction of the leg movement changes and computes the wavelet coefficients to detect FOG events. Publicly available FOG database was used and the technique was evaluated using receiver operating characteristic (ROC) analysis. Results show a higher performance of the wavelet feature in discrimination of the FOG events from the background activity when compared with the existing technique...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28225592/controlling-agglomeration-of-protein-aggregates-for-structure-formation-in-liquid-oil-a-sticky-business
#13
Auke de Vries, Yuly Lopez Gomez, Bas Jansen, Erik van der Linden, Elke Scholten
Proteins are known to be effective building blocks when it comes to structure formation in aqueous environments. Recently, we have shown that submicron colloidal protein particles can also be used to provide structure to liquid oil and form so-called oleogels.1 Preventing extensive agglomeration of these submicron building blocks is a prerequisite to allow for efficient structure formation. This was achieved by applying a solvent exchange procedure to transfer the building blocks from water to oil. The aim of the current paper was to elucidate on the enhanced stability against agglomeration of heat-set whey protein isolate (WPI) aggregates in oil after applying such a solvent exchange procedure...
February 22, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28224887/facile-and-green-fabrication-of-cellulosed-based-aerogels-for-lampblack-filtration-from-waste-newspaper
#14
Peidong Fan, Yali Yuan, Junkai Ren, Bin Yuan, Qian He, Guangmei Xia, Fengxia Chen, Rui Song
In this study, the lightweight, hydrophobic and porous cellulose-based aerogels (CAGs) were synthesized through a freeze-drying process using waste newspaper as the only raw material. After crosslinking with glutaraldehyde and treatment with trimethylchlorosilane (TMCS) using a simple thermal chemical vapor deposition process, the resulting CAGs became hydrophobic and oleophilic. Furthermore, the as-prepared CAGs exhibited a low density (17.4-28.7mgcm(-3)) and mesoporous inner-structure. All these properties attributed the novel aerogel not only with a good adsorption capability of oils and organic solvents, including kerosene, nitrobenzene, and chloroform, but also an excellent filtration capacity of lampblack...
April 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28224610/structural-changes-in-cartilage-and-collagen-studied-by-high-temperature-raman-spectroscopy
#15
Mark Fields, Nicholas Spencer, Jayesh Dudhia, Paul F McMillan
Understanding the high temperature behavior of collagen and collagenous tissue is important for surgical procedures and biomaterials processing for the food, pharmaceutical and cosmetics industries. One primary event for proteins is thermal denaturation that involves unfolding the polypeptide chains while maintaining the primary structure intact. Collagen in the extracellular matrix of cartilage and other connective tissue is a hierarchical material containing bundles of triple-helical fibers associated with water and proteoglycan components...
February 22, 2017: Biopolymers
https://www.readbyqxmd.com/read/28224123/model-based-pat-for-quality-management-in-pharmaceuticals-freeze-drying-state-of-the-art
#16
REVIEW
Davide Fissore
Model-based process analytical technologies can be used for the in-line control and optimization of a pharmaceuticals freeze-drying process, as well as for the off-line design of the process, i.e., the identification of the optimal operating conditions. This paper aims at presenting the state of the art in this field, focusing, particularly, on three groups of systems, namely, those based on the temperature measurement (i.e., the soft sensor), on the chamber pressure measurement (i.e., the systems based on the test of pressure rise and of pressure decrease), and on the sublimation flux estimate (i...
2017: Frontiers in Bioengineering and Biotechnology
https://www.readbyqxmd.com/read/28224001/overexpression-of-prunus-mume-dehydrin-genes-in-tobacco-enhances-tolerance-to-cold-and-drought
#17
Fei Bao, Dongliang Du, Yang An, Weiru Yang, Jia Wang, Tangren Cheng, Qixiang Zhang
Dehydrins, known as group 2 or D-11 family late-embryogenesis-abundant (LEA) proteins, play important roles in plant growth and stress tolerance. Six dehydrin genes were previously identified from the genome of Prunus mume. In this study, five of them (PmLEA8, PmLEA10, PmLEA19, PmLEA20, and PmLEA29) were cloned from cold-resistant P. mume 'Beijingyudie'. Real-time RT-PCR analysis indicated that all these genes could be up-regulated by one or several treatments (ABA, SA, low temperature, high temperature, PEG, and NaCl treatments)...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28223797/fabrication-and-in-vitro-in-vivo-evaluation-of-amorphous-andrographolide-nanosuspensions-stabilized-by-d-%C3%AE-tocopheryl-polyethylene-glycol-1000-succinate-sodium-lauryl-sulfate
#18
Hongzhi Qiao, Lihua Chen, Tianqi Rui, Jingxian Wang, Ting Chen, Tingming Fu, Junsong Li, Liuqing Di
Andrographolide (ADG) is a diterpenoid isolated from Andrographis paniculata with a wide spectrum of biological activities, including anti-inflammatory, anticancer and hepatoprotective effects. However, its poor water solubility and efflux by P-glycoprotein have resulted in lower bioavailability. In this study, ADG nanosuspensions (ADG-NS) were prepared using a wet media milling technique followed by freeze drying. d-α-Tocopheryl polyethylene glycol 1000 succinate (TPGS), a surfactant that inhibits P-glycoprotein function, and sodium lauryl sulfate were used as surface stabilizers...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28223021/trehalose-improves-rabbit-sperm-quality-during-cryopreservation
#19
Zhendong Zhu, Xiaoteng Fan, Yang Pan, Yinghua Lu, Wenxian Zeng
High levels of reactive oxygen species are associated with spermatozoa cryopreservation, which bring damage to functional spermatozoa. The aim of the present study was to investigate whether and how the freezing extenders supplemented with trehalose was beneficial for the survival of rabbit spermatozoa. semen was diluted with Tris-citrate-glucose extender addition of different concentrations of trehalose. Addition of 100 mM trehaose significantly improved post-thaw rabbit sperm parameters, such as motility, intact acrosome, membrane integrity and mitochondrial membrane potential...
February 18, 2017: Cryobiology
https://www.readbyqxmd.com/read/28222256/dynamic-melting-of-freezing-droplets-on-ultra-slippery-superhydrophobic-surfaces
#20
Fuqiang Chu, Xiaomin Wu, Lingli Wang
Condensed droplet freezing and freezing droplet melting phenomena on the prepared ultra-slippery superhydrophobic surface were observed and discussed in this study. Although the freezing delay performance of the surface is common, the melting of the freezing droplets on the surface is quite interesting. Three self-propelled movements of the melting droplets (ice water mixture) were found including the droplet rotating, the droplet jumping and the droplet sliding. The melting droplet rotating, which means that the melting droplet rotates spontaneously on the superhydrophobic surface like a spinning-top, is first reported in this study, and may have some potential applications in various engineering fields...
February 21, 2017: ACS Applied Materials & Interfaces
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