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Luis Anel-López, Olga Garcia-Álvarez, Tatiana Tarantini, David Del Olmo, Jose Antonio Ortiz, Sergio Ledda, Emilio Arsenio Martinez, Ana J Soler, Jordi Roca, María R Fernández Santos, Juan Maria Vázquez, Inmaculada Parrilla, Jose Julián Garde
The aim of this study was to assess the effect of insemination timing on pregnancy rates in red deer (Cervus elaphus) when using sex-sorted sperm samples. Semen was collected by electroejaculation from 8 mature stags and processed to obtain: Conventional samples, following standard freezing procedures for commercial purposes; Control sorted samples, diluted and handled as per sorted samples but without being submitted to the sorter passage; and Y Sex Sorted (YSS) samples. Hinds were synchronized via intravaginal CIDR (Controlled Internal Drug Release) placement and given eCG (Folligon® PMSG Serum Gonadotrophin) on day 12, upon CIDR removal...
March 9, 2018: Theriogenology
Matthieu Fumagalli, Julien Berriot, Benoit de Gaudemaris, Anne Veyland, Jean-Luc Putaux, Sonia Molina-Boisseau, Laurent Heux
Rubber materials with well-dispersed fillers and large mechanical reinforcement have been obtained by melt-processing a diene elastomer matrix and tailored nanocellulose powders having both a high specific surface area and a modified interface. Such filler powders with a specific surface area of 180 m2 g-1 and 100 m2 g-1 have been obtained by freeze-drying suspensions of short needle-like cellulose nanocrystals (CNCs) and entangled networks of microfibrillated cellulose (MFC) in tert-butanol/water, respectively...
March 16, 2018: Soft Matter
Christine D Plant, Giles W Plant
Schwann cells are the primary inducers of regeneration of the peripheral nervous system. Schwann cells can be isolated from adult peripheral nerves, expanded in large numbers, and genetically transduced by viral vectors in vitro prior to their use in vivo. Here we describe how to use lentiviral vectors to transduce primary Schwann cells in vitro. We also describe how cultured Schwann cells can be used in conjunction with decellularized peripheral nerve sheaths prepared by multiple freeze thawing of peripheral nerve tissue...
2018: Methods in Molecular Biology
Evangelos G Papanikolaou, Hakan Yarali, Evi Timotheou, Michael Grynberg, Odysseas Zafeiratis, Herman Tournaye, Robert Najdecki
Introduction: A drawback of gonadotropin-releasing hormone (GnRH) antagonist protocols in in vitro fertilization (IVF) is that they have limited flexibility in cycle programming. This proof of concept study explored the efficacy of a single-dose, long-acting GnRH antagonist IVF protocol. Trial registration number is NCT03240159, retrospectively registered on March 08, 2017. Materials and methods: The efficacy of a single-dose long-acting antagonist, degarelix, was explored initially in healthy donors and subsequently in infertile patients...
2018: Frontiers in Endocrinology
Tao Liu, Karin D Rodland, Richard D Smith
Aberrant degradation of proteins is associated with many pathological states, including cancers. Mass spectrometric analysis of the tumor peptidome has the potential to provide biological insights on proteolytic processing in cancer. However, attempts to use the tumors peptidome information in cancer research have been fairly limited to date, largely due to the lack of effective approaches for robust peptidomics identification and quantification, and the prevalence of confounding factors and biases associated with sample handling and processing...
2018: Vitamins and Hormones
R Petrilli, J O Eloy, J A R Paschoal, R F V Lopez
5-Fluorouracil (5-FU) is used for the topical treatment of pre-cancerous skin lesions. However, previous reports focus on 5-FU quantification in plasma samples, failing to provide information about drug quantification in the skin. Therefore, the aim of this work was to develop a simple and reliable analytical method employing HPLC-UV for 5-FU quantification in skin samples. Chromatographic separation was obtained using the commonly available Lichrospher RP-C18 endcapped column. Porcine ear skin matrix-based analytical curves with thymine, as internal standard, were used...
March 5, 2018: Die Pharmazie
E Lázaro-Lázaro, J A Moreno-Razo, M Medina-Noyola
Upon compression, the equilibrium hard-sphere liquid [pair potential uHS (r)] freezes at a packing fraction ϕf = 0.494 or, if crystallization is prevented, becomes metastable up to its glass transition at ϕg ≈ 0.58. Throughout the fluid regime (ϕ < ϕg ), we are, thus, certain that this model liquid does not exhibit any form of kinetic arrest. If, however, a small portion of these spheres (packing fraction ϕ2 ≪ ϕ) happen to ignore each other [u22 (r) = 0] but do not ignore the remaining "normal" hard spheres [u12 (r) = u21 (r) = u11 (r) = uHS (r)], whose packing fraction is thus ϕ1 = ϕ - ϕ2 , they run the risk of becoming dynamically arrested before they demix from the "normal" particles...
March 14, 2018: Journal of Chemical Physics
Louise Wogelred, Fredrik Höök, Björn Agnarsson, Peter Sjövall
Accurate detection and quantification of individual molecules is important for the development of improved diagnostic methods as well as biochemical characterization of disease progression and treatments. Time-of-flight secondary ion mass spectrometry (ToF-SIMS) is a surface analysis technique capable of imaging the distribution of specific molecules on surfaces with a high spatial resolution (<1 μm) and high sensitivity. ToF-SIMS is particularly suitable for detection of molecules up to ∼2 kDa, including lipids, whereas larger molecules, such as peptides and proteins, are fragmented during analysis, which makes them difficult to identify...
March 15, 2018: Biointerphases
Pavithra Madhavi Naullage, Yuqing Qiu, Valeria Molinero
Cold-adapted organisms produce antifreeze proteins and glycoproteins to control the growth, melting and recrystallization of ice. It has been proposed that these molecules pin the crystal surface, creating a surface curvature that arrests its growth and melting. Here we use thermodynamic modeling and molecular simulations to elucidate how does the curvature of the metastable ice front depend on the temperature and distance between pinned molecules, and what determines the thermal hysteresis on melting and freezing, i...
March 15, 2018: Journal of Physical Chemistry Letters
Thanyaporn Kangwannaronkul, Keskanya Subbalekha, Philaiporn Vivatbutsiri, Jaijam Suwanwela
PURPOSE: Bio-Oss and demineralized freeze-dried bone allograft (DFDBA) are two commercial bone grafts that have been associated with clinical success for many years. However, there are few in vivo studies regarding their healing mechanism. The purpose of this study was to investigate the level of bone formation using microcomputed tomography (micro-CT) and gene expression in mouse calvaria at 1 and 3 months after bone grafting with deproteinized bovine bone and freeze-dried human bone, and compare them to natural bone healing...
March 15, 2018: International Journal of Oral & Maxillofacial Implants
Clarisse Ribeiro, Carlos M Costa, Daniela M Correia, João Nunes-Pereira, Juliana Oliveira, Pedro Martins, Renato Gonçalves, Vanessa F Cardoso, Senentxu Lanceros-Méndez
Poly(vinylidene fluoride) (PVDF) and its copolymers are the polymers with the highest dielectric constants and electroactive responses, including piezoelectric, pyroelectric and ferroelectric effects. This semicrystalline polymer can crystallize in five different forms, each related to a different chain conformation. Of these different phases, the β phase is the one with the highest dipolar moment and the highest piezoelectric response; therefore, it is the most interesting for a diverse range of applications...
April 2018: Nature Protocols
Zhen Ma, Jinghui Wang, Zhao-Yang Dong, Jun Zhang, Shichao Li, Shu-Han Zheng, Yunjie Yu, Wei Wang, Liqiang Che, Kejing Ran, Song Bao, Zhengwei Cai, P Čermák, A Schneidewind, S Yano, J S Gardner, Xin Lu, Shun-Li Yu, Jun-Ming Liu, Shiyan Li, Jian-Xin Li, Jinsheng Wen
We report on comprehensive results identifying the ground state of a triangular-lattice structured YbZnGaO_{4} as a spin glass, including no long-range magnetic order, prominent broad excitation continua, and the absence of magnetic thermal conductivity. More crucially, from the ultralow-temperature ac susceptibility measurements, we unambiguously observe frequency-dependent peaks around 0.1 K, indicating the spin-glass ground state. We suggest this conclusion holds also for its sister compound YbMgGaO_{4}, which is confirmed by the observation of spin freezing at low temperatures...
February 23, 2018: Physical Review Letters
Youmin Hou, Miao Yu, Yuhe Shang, Peng Zhou, Ruyuan Song, Xiaonan Xu, Xuemei Chen, Zuankai Wang, Shuhuai Yao
Preventing or minimizing ice formation in supercooled water is of prominent importance in many infrastructures, transportation, and cooling systems. The overall phase change heat transfer on icephobic surfaces, in general, is intentionally sacrificed to suppress the nucleation of water and ice. However, in a condensation frosting process, inhibiting freezing without compromising the water condensation has been an unsolved challenge. Here we show that this conflict between anti-icing and efficient condensation cooling can be resolved by utilizing biphilic topography with patterned high-contrast wettability...
February 16, 2018: Physical Review Letters
Kathryn E Drzewiecki, Juilee N Malavade, Ijaz Ahmed, Christopher J Lowe, David I Shreiber
As a biomaterial, collagen has been used throughout tissue engineering and regenerative medicine. Collagen is native to the body, is highly biocompatible, and naturally promotes cell adhesion and regeneration. However, collagen fibers and the inherent weak mechanical properties of collagen hydrogels interfere with further development of collagen as a bio-ink. Herein, we demonstrate the use of a modified type-I collagen, collagen methacrylamide (CMA), as a fibril-forming bio-ink for free-form fabrication of scaffolds...
December 2017: Technology
Helen V Wilmot, Jason Hockley, Peter Rigsby, Elaine Gray
Until recently, the role of factor XII (FXII) in hemostasis was not considered to be important since patients with FXII deficiency do not present with bleeding. The activation of FXII by agents including mast cells and platelet polyphosphates suggests that it may have a role in thrombogenesis. The inhibition of FXII therefore presents an option for antithrombotic therapy, and antibodies and inhibitors are already in development. Assays for FXII will be required to support these technologies, and an international standard (IS) for FXII would be useful for the development of these methods and for the clinical monitoring of patients...
2018: Frontiers in Medicine
Ruwei Ou, Qianqian Wei, Bei Cao, Wei Song, Yanbing Hou, Hui Liu, Xiaoqin Yuan, Bi Zhao, Ying Wu, Huifang Shang
Objective: To explore the clinical predictors of freezing of gait (FOG) in Chinese patients with Parkinson's disease (PD). Methods: This study included 225 patients with PD who completed a three-year follow-up visit. The end-point was the presence of FOG (freezers), which was assessed during the follow-up visit. Group comparisons were conducted, followed by a further forward binary logistic regression analysis. Results: Eighty-five patients with PD (38%) had developed FOG at the end of study...
March 2018: Brain and Behavior
Jiayue Cai, Soojin Lee, Fang Ba, Saurabh Garg, Laura J Kim, Aiping Liu, Diana Kim, Z Jane Wang, Martin J McKeown
Falls and balance difficulties remain a major source of morbidity in Parkinson's Disease (PD) and are stubbornly resistant to therapeutic interventions. The mechanisms of gait impairment in PD are incompletely understood but may involve changes in the Pedunculopontine Nucleus (PPN) and its associated connections. We utilized fMRI to explore the modulation of PPN connectivity by Galvanic Vestibular Stimulation (GVS) in healthy controls ( n = 12) and PD subjects even without overt evidence of Freezing of Gait (FOG) while on medication ( n = 23)...
2018: Frontiers in Neuroscience
E Damm, D Bauch, T Krumpen, B Rabe, M Korhonen, E Vinogradova, C Uhlig
Methane sources and sinks in the Arctic are poorly quantified. In particular, methane emissions from the Arctic Ocean and the potential sink capacity are still under debate. In this context sea ice impact on and the intense cycling of methane between sea ice and Polar surface water (PSW) becomes pivotal. We report on methane super- and under-saturation in PSW in the Eurasian Basin (EB), strongly linked to sea ice-ocean interactions. In the southern EB under-saturation in PSW is caused by both inflow of warm Atlantic water and short-time contact with sea ice...
March 14, 2018: Scientific Reports
Tao-Mian Mi, Shan-Shan Mei, Pei-Peng Liang, Lin-Lin Gao, Kun-Cheng Li, Tao Wu, Piu Chan
A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has not been fixed in the paper.
March 14, 2018: Scientific Reports
Kai Zhao, Yuzhen Zhou, Sagheer Ahmad, Xue Yong, Xuehua Xie, Yu Han, Yushu Li, Lidan Sun, Qixiang Zhang
The survival in freezing temperature for woody plants is exclusively dependent on the perception of coldness and induction of dormancy. CBF/DREB1 transcriptional factors join cold-response conduits and the DAM genes, especially PmDAM6, are well-known regulators of dormancy. Despite the immense importance, little is documented on the association between CBF proteins and the complexity of the promoter region in PmDAM6 with the function of bud dormancy in P. mume. Therefore, this study was based on the cloning of PmDAM6 and six PmCBFs to evaluate their integral roles in the process of bud development...
March 14, 2018: Scientific Reports
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