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https://www.readbyqxmd.com/read/27920525/fabrication-and-characterization-of-drug-loaded-nano-hydroxyapatite-polyamide-66-scaffolds-modified-with-carbon-nanotubes-and-silk-fibroin
#1
Meng-Zhu Yao, Ming-Yi Huang-Fu, Hui-Na Liu, Xia-Rong Wang, Xiaoxia Sheng, Jian-Qing Gao
Nano-hydroxyapatite/polyamide 66 (nHA/PA66) porous scaffolds were fabricated by a phase inversion method. Carbon nanotubes (CNTs) and silk fibroin (SF) were used to modify the surface of the nHA/PA66 scaffolds by freeze-drying and cross-linking. Dexamethasone was absorbed to the CNTs to promote the osteogenic differentiation of bone mesenchymal stem cells (BMSCs). The cell viability of BMSCs was investigated by changing the concentration of the CNT dispersion, and the most biocompatible scaffold was selected...
2016: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/27919994/clinical-outcome-of-reconstruction-using-frozen-autograft-for-a-humeral-bone-tumor
#2
Hiroaki Kimura, Norio Yamamoto, Toshiharu Shirai, Hideji Nishida, Katsuhiro Hayashi, Yoshikazu Tanzawa, Akihiko Takeuchi, Shinji Miwa, Hiroyuki Tsuchiya
BACKGROUND: We developed a frozen autograft technique in which a tumor-bearing bone was treated with liquid nitrogen for reconstruction. The purpose of this study was to evaluate the functional outcomes of this technique after resection of humeral bone tumors. PATIENTS AND METHODS: Eight patients were included in the study. The freezing range was classified into 2 groups, depending on whether the joint surface of the humeral head was frozen. RESULTS: In the joint freezing group, the mean active shoulder abduction and flexion were 43...
December 2016: Anticancer Research
https://www.readbyqxmd.com/read/27918929/long-term-red-meat-preservation-using-chilled-and-frozen-storage-combinations-a-review
#3
REVIEW
Cassius E O Coombs, Benjamin W B Holman, Michael A Friend, David L Hopkins
This paper reviews current literature relating to the effects of chilled and frozen storage on the quality characteristics of red meat (lamb and beef). These characteristics include tenderness (shear force), juiciness (fluid losses), flavour (lipid oxidation), colour and microbial loading. Research suggests that chilled storage of red meat can improve certain properties, such as shear force and species-specific flavour, to threshold levels before the effects on flavour and colour become deleterious, and key microbial species proliferate to spoilage levels...
December 1, 2016: Meat Science
https://www.readbyqxmd.com/read/27917410/time-lapse-in-situ-imaging-of-ice-crystal-growth-using-confocal-microscopy
#4
Moreno Marcellini, Cecile Noirjean, Dmytro Dedovets, Juliette Maria, Sylvain Deville
Ice crystals nucleate and grow when a water solution is cooled below its freezing point. The growth velocities and morphologies of the ice crystals depend on many parameters, such as the temperature of ice growth, the melting temperature, and the interactions of solutes with the growing crystals. Three types of morphologies may appear: dendritic, cellular (or fingerlike), or the faceted equilibrium form. Understanding and controlling which type of morphology is formed is essential in several domains, from biology to geophysics and materials science...
November 30, 2016: ACS Omega
https://www.readbyqxmd.com/read/27916810/is-the-geographic-range-of-mangrove-forests-in-the-conterminous-united-states-really-expanding
#5
Chandra Giri, Jordan Long
Changes in the distribution and abundance of mangrove species within and outside of their historic geographic range can have profound consequences in the provision of ecosystem goods and services they provide. Mangroves in the conterminous United States (CONUS) are believed to be expanding poleward (north) due to decreases in the frequency and severity of extreme cold events, while sea level rise is a factor often implicated in the landward expansion of mangroves locally. We used ~35 years of satellite imagery and in situ observations for CONUS and report that: (i) poleward expansion of mangrove forest is inconclusive, and may have stalled for now, and (ii) landward expansion is actively occurring within the historical northernmost limit...
November 28, 2016: Sensors
https://www.readbyqxmd.com/read/27916403/stability-of-synthetic-cathinones-in-oral-fluid-samples
#6
Briana Miller, Jiyoung Kim, Marta Concheiro
Synthetic cathinones are new stimulant drugs derived from cathinone that have been sold as "legal highs" worldwide. These compounds can elicit powerful effects such as delusions, hallucinations as well as other potentially dangerous behavior. New analogs with varying effects and potencies are constantly introduced in the market to evade legislation, and they are not detected by routine screening and confirmation methods. Oral fluid is an alternative matrix of increasing interest in forensic toxicology. Its collection is non-invasive and easily supervised, and positive drug findings typically reflect recent drug exposure...
December 1, 2016: Forensic Science International
https://www.readbyqxmd.com/read/27915492/preparation-and-in-vitro-evaluation-of-melatonin-loaded-ha-pva-gelformulations
#7
Sakine Tuncay Tanrıverdi, Catalina Natalia Cheaburu-Yilmaz, Sonia Carbone, Özgen Özer
Melatonin loaded hyaluronic acid and poly(vinyl alcohol) gels were prepared by using freeze-thaw technique and an emulsion method followed by freeze-thaw technique to produce a new synergistic system for topical application. Freeze-thaw hydrogels and emulgels were characterized by means of Fourier Transform Infrared Spectroscopy, rheology and swelling tests. The porous structure of the hydrogels was shown by scanning electron microscopy observations and thermal properties were tested by Differential Scanning Calorimetry measurements...
December 5, 2016: Pharmaceutical Development and Technology
https://www.readbyqxmd.com/read/27915412/formation-of-indigoidine-derived-pigments-contributes-to-the-adaptation-of-vogesella-sp-strain-eb-to-cold-aquatic-iron-oxidizing-environments
#8
Paula Arrúa Day, María S Villalba, O Marisa Herrero, Luz Alejandra Arancibia, Héctor M Alvarez
We investigated previously under explored cold aquatic environments of Andean Patagonia, Argentina. Oily sheens similar to an oil spill are frequently observed at the surface of water in creeks and small ponds in these places. Chemical analysis of a water sample revealed the occurrence of high concentrations of iron and the presence of a free insoluble indigoidine-derived pigment. A blue pigment-producing bacterium (strain EB) was isolated from the water sample and identified as Vogesella sp. by molecular analysis...
December 3, 2016: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/27915226/subthalamic-nucleus-stimulation-and-gait-in-parkinson-s-disease-a-not-always-fruitful-relationship
#9
REVIEW
Giovanni Cossu, Massimiliano Pau
Deep brain stimulation (DBS) of the subthalamic nucleus (STN) provides efficient treatment for the alleviation of motor signs in patients with advanced Parkinson's disease (PD), but its specific effects on gait is sometimes less successful as it may even lead to an aggravation of freezing of gait. To better understand when axial symptoms can be expected to improve and when they may worsen or be resistant to STN-DBS, we propose here a narrative review that considers the recent literature evidences based on instrumental gait analysis data...
November 27, 2016: Gait & Posture
https://www.readbyqxmd.com/read/27914794/modeling-the-secondary-drying-stage-of-freeze-drying-development-and-validation-of-an-excel-based-model
#10
Ekneet K Sahni, Michael J Pikal
Although several mathematical models of primary drying have been developed over the years, with significant impact on the efficiency of process design, models of secondary drying have been confined to highly complex models. The simple-to-use Excel-based model developed here is, in essence, a series of steady state calculations of heat and mass transfer in the 2 halves of the dry layer where drying time is divided into a large number of time steps, where in each time step steady state conditions prevail. Water desorption isotherm and mass transfer coefficient data are required...
November 30, 2016: Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/27913993/antifreeze-glycopeptides-from-structure-and-activity-studies-to-current-approaches-in-chemical-synthesis
#11
REVIEW
Małgorzata Urbańczyk, Jerzy Góra, Rafał Latajka, Norbert Sewald
Antifreeze glycopeptides (AFGPs) are a class of biological antifreeze agents found predominantly in Arctic and Antarctic species of fish. They possess the ability to regulate ice nucleation and ice crystal growth, thus creating viable life conditions at temperatures below the freezing point of body fluids. AFGPs usually consist of 4-55 repetitions of the tripeptide unit Ala-Ala-Thr that is O-glycosylated at the threonine side chains with β-D-galactosyl-(1 → 3)-α-N-acetyl-D-galactosamine. Due to their interesting properties and high antifreeze activity, they have many potential applications, e...
December 2, 2016: Amino Acids
https://www.readbyqxmd.com/read/27913953/neuroinflammation-induced-downregulation-of-hippocampacal-neuregulin-1-erbb4-signaling-in-the-parvalbumin-interneurons-might-contribute-to-cognitive-impairment-in-a-mouse-model-of-sepsis-associated-encephalopathy
#12
Rong Gao, Mu-Huo Ji, Da-Peng Gao, Run-Hua Yang, Shao-Gang Zhang, Jian-Jun Yang, Jin-Chun Shen
Sepsis-associated encephalopathy (SAE) is a common complication associated with poor prognosis in septic patients, but the underlying mechanism remains unclear. We hypothesized that disturbed neuregulin 1 (NRG1)-ErbB4 signaling in the parvalbumin interneurons was involved in sepsis-induced cognitive impairment in a mouse model of SAE. The SAE model was induced by cecal ligation/perforation (CLP). Animals were randomly divided into the following six groups: sham + vehicle group, sham + NRG1 group, CLP + vehicle group, CLP + NRG1 group, CLP + NRG1 + AG1478 (ErbB4 inhibitor) group, and CLP + minocycline group...
December 2, 2016: Inflammation
https://www.readbyqxmd.com/read/27912011/reduced-snow-cover-alters-root-microbe-interactions-and-decreases-nitrification-rates-in-a-northern-hardwood-forest
#13
Patrick O Sorensen, Pamela H Templer, Lynn Christenson, Jorge Duran, Timothy Fahey, Melany C Fisk, Peter M Groffman, Jennifer L Morse, Adrien C Finzi
Snow cover is projected to decline during the next century in many ecosystems that currently experience a seasonal snowpack. Because snow insulates soils from frigid winter air temperatures, soils are expected to become colder and experience more winter soil freeze-thaw cycles as snow cover continues to decline. Tree roots are adversely affected by snowpack reduction, but whether loss of snow will affect root-microbe interactions remains largely unknown. The objective of this study was to distinguish and attribute direct (e...
December 2016: Ecology
https://www.readbyqxmd.com/read/27911563/codecaying-dark-matter
#14
Jeff Asaf Dror, Eric Kuflik, Wee Hao Ng
We propose a new mechanism for thermal dark matter freeze-out, called codecaying dark matter. Multicomponent dark sectors with degenerate particles and out-of-equilibrium decays can codecay to obtain the observed relic density. The dark matter density is exponentially depleted through the decay of nearly degenerate particles rather than from Boltzmann suppression. The relic abundance is set by the dark matter annihilation cross section, which is predicted to be boosted, and the decay rate of the dark sector particles...
November 18, 2016: Physical Review Letters
https://www.readbyqxmd.com/read/27911340/dementia-predictors-in-parkinson-disease-a-validation-study
#15
Julius Bm Anang, Takashi Nomura, Silvia Rios Romenets, Kenji Nakashima, Jean-Francois Gagnon, Ronald B Postuma
In a prospective study, we recently discovered 8 clinical predictors of dementia in Parkinson's disease. Here, we validate these dementia predictors using two additional prospective cohorts (n = 134). After a 3.6-year follow-up, 35/134 developed dementia (7.2% per year). When confirming individual variables, 5/8 were significantly associated with dementia in the validation cohort. These included age, male sex, baseline RBD, orthostatic hypotension, and MCI. Bilateral onset, hallucinations and falls/freezing did not significantly predict dementia; however, point estimates of OR were all >1...
November 30, 2016: Journal of Parkinson's Disease
https://www.readbyqxmd.com/read/27909862/combined-characterization-of-bovine-polyhemoglobin-microcapsules-by-uv-vis-absorption-spectroscopy-and-cyclic-voltammetry
#16
Marcos Camargo Knirsch, Filippo Dell'Anno, Marco Salerno, Claudio Larosa, Bronislaw Polakiewicz, Roberto Eggenhöffner, Attilio Converti
Polyhemoglobin produced from pure bovine hemoglobin by reaction with PEG bis(N-succynimidil succinate) as a cross-linking agent was encapsulated in gelatin and dehydrated by freeze-drying. Free carboxyhemoglobin and polyhemoglobin microcapsules were characterized by UV-Vis spectroscopy in the absorption range 450-650 nm and cyclic voltammetry in the voltage range from -0.8 to 0.6 mV to evaluate the ability to break the bond with carbon monoxide and to study the carrier's affinity for oxygen, respectively...
December 1, 2016: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/27909755/leachate-properties-and-cadmium-migration-through-freeze-thaw-treated-soil-columns
#17
Meng Xu, Yue Zheng, Weiwei Chen, Na Mao, Ping Guo
Soil column leaching experiments were conducted to study the effects of multiple freeze-thaw cycles on the vertical migration of cadmium (Cd). Three Cd-spiked leaching solutions of different properties were derived from snowmelt, sludge, and straw, designated as B, W and J, respectively. The leaching solutions varied in dissolved organic matter (DOM) concentrations in the order of J > W > B. Changes in leachate properties and Cd concentration were observed. The results showed that pH values of all the leachate solutions through freeze-thaw treated soil columns were higher than those of leachates through unfrozen soils...
December 1, 2016: Bulletin of Environmental Contamination and Toxicology
https://www.readbyqxmd.com/read/27908713/optimization-of-resistant-starch-formation-from-high-amylose-corn-starch-by-microwave-irradiation-treatments-and-characterization-of-starch-preparations
#18
Selime Mutlu, Kevser Kahraman, Serpil Öztürk
The effects of microwave irradiation on resistant starch (RS) formation and functional properties in high-amylose corn starch, Hylon VII, by applying microwave-storing cycles and drying processes were investigated. The Response Surface Methodology (RSM) was used to optimize the reaction conditions, microwave time (2-4min) and power (20-100%), for RS formation. The starch:water (1:10) mixtures were cooked and autoclaved and then different microwave-storing cycles and drying (oven or freeze drying) processes were applied...
November 28, 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27908687/fertility-response-evaluation-of-artificial-insemination-methods-in-sheep-with-fresh-and-frozen-thawed-semen
#19
Reza Masoudi, Ahmad Zare Shahneh, Armin Towhidi, Hamid Kohram, Abbas Akbarisharif, Mohsen Sharafi
The aim of this study was to evaluate the fertility response of artificial insemination (AI) methods with fresh and frozen sperm in sheep. In experiment 1, one hundred and fifty fat tailed Zandi ewes were assigned into 3 equal groups and inseminated with three AI methods consisting of vaginal, laparoscopic and trans-cervical AI with fresh semen. In experiment 2, a factorial study (3 AI methods × 2 extenders) was used to analyze the effects of three AI methods and two freezing extenders containing soybean lecithin (SL) or Egg yolk (EY) on reproductive performance of 300 fat tailed Zandi ewes...
November 28, 2016: Cryobiology
https://www.readbyqxmd.com/read/27908685/royal-jelly-supplemented-soybean-lecithin-based-extenders-improve-post-thaw-quality-and-incubation-resilience-of-goat-spermatozoa
#20
Selim Alcay, M Berk Toker, N Tekin Onder, Elif Gokce
The aim of the present study was to evaluate different concentrations of royal jelly (RJ) supplemented extenders for post-thaw quality and incubation resilience of goat spermatozoa. Semen samples were collected from five goats. Pooled semen were diluted with soybean lecithin-based extender without RJ (control) or supplemented with different concentrations (0.25, 0.5 and 0.75%) of RJ (RJ0.25, RJ0.5, RJ0.75 respectively), at a final concentration of 150 × 10(6) spermatozoon/mL. Semen samples were assessed for sperm motility, plasma membrane integrity using hypoosmotic swelling test (HOST) damaged acrosome using FITC-Pisum sativum agglutinin (PSA-FITC) and DNA integrity using terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL)...
November 28, 2016: Cryobiology
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