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Eun-Sun Hwang, Nhuan Do Thi
The objective of this study was to determine the effects of different growing regions on quality characteristics, total bioactive compound contents, and in vitro antioxidant activity in aronia. Aronia grown in 3 different regions (Sangjoo, Ulju, and Youngcheon) in Korea was obtained and used fresh or as a freeze-dried powder. No statistically significant differences were observed for moisture, ash, crude lipid, and crude protein contents in aronia sampled from the 3 different regions. Aronia grown in Sangjoo had the highest total acid content and the lowest sugar content and pH value...
September 2016: Preventive Nutrition and Food Science
Petra Kraus, Thomas Lufkin
The adult bovine (Bos taurus) intervertebral disc is primarily comprised of two major tissue types: The outer annulus fibrosus (AF) and the central nucleus pulposus (NP). We isolated several primary cell lineages of passage (P) 0 cells from the AF tissue omitting typically used enzymatic tissue digestion protocols. The cells grow past p10 without signs of senescence in DMEM + 10% FCS on 0.1% gelatin coated/uncoated surfaces of standard cell culture plates and survive freeze-thawing. Preliminary analysis of the AF derived cells for expression of the two structural genes Col1a1 and Col2a1 was performed by PISH recapitulating the expression observed in vivo...
December 2016: Genomics Data
Katharina Kaufmann, Peter Dohmen
BACKGROUND: Literature data indicate that terrestrial life stages of amphibians may be more sensitive to xenobiotics than birds or mammals. It is hypothesized that dermal exposure could potentially be a significant route of exposure for amphibians, as there is evidence that their skin is more permeable than the skin of other vertebrate species. Thus, higher amounts of xenobiotics might enter systemic circulation by dermal uptake resulting in adverse effects. Heretofore, no guidelines exist to investigate dermal toxicity of chemicals to amphibians...
2016: Environmental Sciences Europe
J E LeDoux, J Moscarello, R Sears, V Campese
Research on avoidance conditioning began in the late 1930s as a way to use laboratory experiments to better understand uncontrollable fear and anxiety. Avoidance was initially conceived of as a two-factor learning process in which fear is first acquired through Pavlovian aversive conditioning (so-called fear conditioning), and then behaviors that reduce the fear aroused by the Pavlovian conditioned stimulus are reinforced through instrumental conditioning. Over the years, criticisms of both the avoidance paradigm and the two-factor fear theory arose...
October 18, 2016: Molecular Psychiatry
C Tonus, D Connan, O Waroux, B Vandenhove, J Wayet, L Gillet, D Desmecht, N Antoine, F J Ectors, L Grobet
In the present study, we compare a classical slow freezing (SLF) method and an aseptic vitrification (Vitrif) technique to cryopreserve a stable primordial germ cell (PGCs) line issued from the Ardennaise chicken breed. Viability immediately after warming was close to 80% and did not differ between the two cryopreservation methods. Proliferation tended to be slower for both cryopreservation methods compared with controls, but the difference was significant only for Vitrif. No difference was found between the two methods after flow cytometry analysis of stage-specific embryonic antigen-1 expression and reverse transcription-polymerase chain reaction on several factors related to PGC phenotype...
September 21, 2016: Theriogenology
Rafeeque R Alyethodi, Rajib Deb, Rani Alex, Sushil Kumar, Umesh Singh, Shrikant Tyagi, D K Mandal, T V Raja, A K Das, Sheetal Sharma, Gyanendra S Sengar, Rani Singh, B Prakash
Cryopreservation is one of the most important aspects of frozen semen technology and livestock breeding. Uses of candidate molecular markers in selection strategies for male fertility are well recognized. The present investigation targeted two microsatellite markers (BM1500 and UMN 2008) for association with semen quality variables and freezing capacity in Frieswal (HF×Sahiwal) crossbred bulls of Indian origin. Of the different alleles at the polymorphic locus BM1500, the 136bp allele was associated with greater (P<0...
October 11, 2016: Animal Reproduction Science
Xiang Liu, Quan Wang, Zhiming Qi, Jiangang Han, Lanhai Li
In the last decade, an increasing number of studies have reported that soil nitrous oxide (N2O) emissions can be reduced by adding biochar. However, the effect of biochar amendment on soil N2O emissions during freeze-thaw cycle (FTC) is still unknown. In this laboratory study, biochar (0%, 2% and 4%, w/w) was added into a cultivated sandy loam soil and then treated with 15 times of FTC (each FTC consisted of freeze at -5/-10 °C for 24 h and thaw at 5/10 °C for 24 h), to test whether biochar can mitigate soil N2O emissions during FTC, and estimate the relationships between N2O emissions and soil inorganic nitrogen contents/microbial biomass content/enzyme activities...
October 17, 2016: Scientific Reports
Alexandros Koutsioubas
Supported lipid bilayers on planar surfaces constitute an archetypical experimental system for the study of biological membranes. The popularity of these ordered molecular layers in the literature, is on one hand related to the simplicity of their preparation using the method of vesicle fusion and on the other hand to their compatibility with a multitude of surface sensitive experimental probes. Neutron reflectivity has proven as an important experimental method for the investigation of such systems with the ability to provide sub-nanometer structural information perpendicular to the supporting plane...
October 17, 2016: Journal of Physical Chemistry. B
Y Lee, D K Lee, J M Lee, S J Chung, J J Lee, Y H Sohn, P H Lee
BACKGROUND AND PURPOSE: Although early cerebellar symptoms are one of the exclusive criteria in the diagnosis of progressive supranuclear palsy (PSP), cerebellar involvement in PSP is evident both clinically and pathologically. However, structural analysis focusing on the cerebellum has not been previously studied in patients with PSP. We aimed to evaluate cerebellar involvement in PSP using a magnetic resonance imaging-based segmental volumetric analysis. METHODS: We retrospectively enrolled 48 patients with PSP composed of 25 patients with PSP-Richardson's syndrome (RS) and 23 patients with pure akinesia with gait freezing, 39 patients with Parkinson's disease (PD) and 34 healthy controls...
October 16, 2016: European Journal of Neurology: the Official Journal of the European Federation of Neurological Societies
Islam I Sabike, Ryoko Uemura, Yumi Kirino, Hirohisa Mekata, Satoshi Sekiguchi, Tamaki Okabayashi, Yoshitaka Goto, Wataru Yamazaki
Rapid identification of Campylobacter-positive flocks before slaughter, following freezing and heat treatment for the Campylobacter-positive carcasses at the slaughterhouses is an effective control strategy against foodborne campylobacteriosis. We evaluated a loop-mediated isothermal amplification (LAMP) assay for the direct screening of naturally contaminated chicken cloacal swabs for C. jejuni/C. coli to compare this assay with conventional quantitative culture methods. In a comparison study of 165 broilers, the LAMP assay showed 82...
2016: Frontiers in Microbiology
Paramasivam Murugan, Lizhu Han, Chee-Yuen Gan, Frans H J Maurer, Kumar Sudesh
Bacterial polyhydroxyalkanoates (PHA) are expensive partly due to the recovery and purification processes. Thus, many studies have been carried out in order to minimize the cost. Here we report on the use of mealworm, which is the larva of mealworm beetle (Tenebrio molitor) to recover PHA granules from Cupriavidus necator. Mealworms were shown to readily consume the freeze-dried C. necator cells and excrete the PHA granules in the form of whitish feces. Further purification using water, detergent and heat resulted in almost 100% pure PHA granules...
October 13, 2016: Journal of Biotechnology
Amanda C Sharko, Jim R Fadel, Kris F Kaigler, Marlene A Wilson
Identifying the neurobiological mechanisms that underlie differential sensitivity to stress is critical for understanding the development and expression of stress-induced disorders, such as post-traumatic stress disorder (PTSD). Preclinical studies have suggested that rodents display different phenotypes associated with extinction of Pavlovian conditioned fear responses, with some rodent populations being resistant to extinction. An emerging literature also suggests a role for orexins in the consolidation processes associated with fear learning and extinction...
October 13, 2016: Physiology & Behavior
Enrico Papaleo, Luca Pagliardini, Valeria Stella Vanni, Diana Delprato, Patrizia Rubino, Massimo Candiani, Paola Viganò
A cost analysis covering direct healthcare costs relating to IVF freeze-all policy was conducted. Normal- and high- responder patients treated with a freeze-all policy (n = 63) compared with fresh transfer IVF (n = 189) matched by age, body mass index, duration and cause of infertility, predictive factors for IVF (number of oocytes used for fertilization) and study period, according to a 1:3 ratio were included. Total costs per patient (€6952 versus €6863) and mean costs per live birth were similar between the freeze-all strategy (€13,101, 95% CI 10,686 to 17,041) and fresh transfer IVF (€15,279, 95% CI 13,212 to 18,030)...
October 3, 2016: Reproductive Biomedicine Online
Paul S Shamble, Gil Menda, James R Golden, Eyal I Nitzany, Katherine Walden, Tsevi Beatus, Damian O Elias, Itai Cohen, Ronald N Miles, Ronald R Hoy
Jumping spiders (Salticidae) are famous for their visually driven behaviors [1]. Here, however, we present behavioral and neurophysiological evidence that these animals also perceive and respond to airborne acoustic stimuli, even when the distance between the animal and the sound source is relatively large (∼3 m) and with stimulus amplitudes at the position of the spider of ∼65 dB sound pressure level (SPL). Behavioral experiments with the jumping spider Phidippus audax reveal that these animals respond to low-frequency sounds (80 Hz; 65 dB SPL) by freezing-a common anti-predatory behavior characteristic of an acoustic startle response...
October 6, 2016: Current Biology: CB
Adam Balkham, Sarah Alderson
BACKGROUND: The introduction of the Health and Social Care Bill (2011) changed the role of GPs to include commissioning of health services. Aim This study aimed to identify any differences in the media portrayal of GPs before and after the introduction of the Bill. METHODS: We retrospectively searched four British newspapers over the period 2009-2013 using the media database Nexis. In order to directly compare the findings of the study with the work of Tanner et al...
October 17, 2016: Primary Health Care Research & Development
Donatella Canistro, Fabio Vivarelli, Silvia Cirillo, Guglielmo Costa, Carlo Andreotti, Moreno Paolini
Over the past years, there has been a growing interest in the natural constituents of foods as a potential means of cancer control. To date, epidemiology studies seem to indicate an inverse association between regular consumption of fruit and vegetables and cancer risk. Here, the potential chemopreventive activity of the polyphenolic extract (PPE) of peach (Prunus persica L. Batsch) and of the freeze-dried fruit in toto (LFT), focusing on the modulation of xenobiotic metabolizing enzymes (XMEs) in vivo, was investigated...
October 11, 2016: Food and Chemical Toxicology
Elise Verger, Pierre Drion, Geneviève Meffre, Claire Bernard, Luc Duwez, Nicolas Lepareur, Olivier Couturier, François Hindré, Roland Hustinx, Franck Lacoeuille
PURPOSE: This work aims to develop, validate and optimize the radiolabeling of Starch-Based Microparticles (SBMP) by 188Re and 68Ga in the form of ready-to-use radiolabeling kits, the ultimate goal being to obtain a unique theranostic vector for the treatment of Hepatocellular Carcinoma. METHODS: Optimal labeling conditions and composition of freeze-dried kits were defined by monitoring the radiochemical purity while varying several parameters. In vitro stability studies were carried out, as well as an in vivo biodistribution as a preliminary approach with the intra-arterial injection of 68Ga radiolabeled SBMP into the hepatic artery of DENA-induced rats followed by PET/CT imaging...
2016: PloS One
Touseef Habib, Dinesh Sundaravadivelu Devarajan, Fardin Khabaz, Dorsa Parviz, Thomas C Achee, Rajesh Khare, Micah J Green
Despite a range of promising applications, liquid-phase exfoliation of boron nitride nanosheets (BNNSs) is limited, both by low yield in common solvents as well as the disadvantages of using dissolved surfactants. One recently reported approach is the use of co-solvent systems to increase the as-obtained concentration of BNNS; the role of these solvents in aiding exfoliation and/or aiding colloidal stability of BNNSs is difficult to distinguish. In this paper, we have investigated the use of a t-butanol/water co-solvent to disperse BNNSs...
October 14, 2016: Langmuir: the ACS Journal of Surfaces and Colloids
Carsten Svaneborg, Hossein Ali Karimi-Varzaneh, Nils Hojdis, Frank Fleck, Ralf Everaers
We present an effective and simple multiscale method for equilibrating Kremer Grest model polymer melts of varying stiffness. In our approach, we progressively equilibrate the melt structure above the tube scale, inside the tube and finally at the monomeric scale. We make use of models designed to be computationally effective at each scale. Density fluctuations in the melt structure above the tube scale are minimized through a Monte Carlo simulated annealing of a lattice polymer model. Subsequently the melt structure below the tube scale is equilibrated via the Rouse dynamics of a force-capped Kremer-Grest model that allows chains to partially interpenetrate...
September 2016: Physical Review. E
Steven Atkinson, Frank H Stillinger, Salvatore Torquato
The nonequilibrium process by which hard-particle systems may be compressed into disordered, jammed states has received much attention because of its wide utility in describing a broad class of amorphous materials. While dynamical signatures are known to precede jamming, the task of identifying static structural signatures indicating the onset of jamming have proven more elusive. The observation that compressing hard-particle packings towards jamming is accompanied by an anomalous suppression of density fluctuations (termed "hyperuniformity") has paved the way for the analysis of jamming as an "inverted critical point" in which the direct correlation function c(r), rather than the total correlation function h(r), diverges...
September 2016: Physical Review. E
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