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https://www.readbyqxmd.com/read/29144754/immunogenic-cell-death-amplified-by-co-localized-adjuvant-delivery-for-cancer-immunotherapy
#1
Yuchen Fan, Rui Kuai, Yao Xu, Lukasz J Ochyl, Darrell J Irvine, James Moon
Despite their potential, conventional whole-cell cancer vaccines prepared by freeze-thawing or irradiation have shown limited therapeutic efficacy in clinical trials. Recent studies have shown that cancer cells treated with certain chemotherapeutics, such as mitoxantrone, can undergo immunogenic cell death (ICD) and initiate anti-tumor immune responses. However, it remains unclear how ICD can be exploited for cancer immunotherapy. Here, we present a new biomaterial-based strategy for converting immunogenically dying tumor cells into a powerful platform for cancer vaccination and demonstrate their immunogenicity in murine models of melanoma and colon carcinoma...
November 16, 2017: Nano Letters
https://www.readbyqxmd.com/read/29143965/display-stability-of-fresh-and-thawed-lamb-supplemented-with-vitamin-e-or-sprayed-with-an-antioxidant-borage-seed-extract
#2
M Bellés, V Alonso, P Roncalés, J A Beltrán
BACKGROUND: The commercialization of thawed lamb packaged in modified atmosphere and maintained on display could serve as an alternative capable of satisfying the requirements of customers as well as distributors. However, previous studies have suggested that lipid oxidation may accelerate post-thawing, as peroxidation occurs during frozen storage, thereby leading to rapid and severe secondary lipid oxidation. The addition of an antioxidant compound either in the lamb diet or in the packaged meat could resolve this problem...
November 16, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/29143694/cryo-scanning-electron-microscopy-sem-and-scanning-transmission-electron-microscopy-stem-in-sem-for-bio-and-organo-mineral-interface-characterization-in-the-environment
#3
Guillaume Wille, Jennifer Hellal, Patrick Ollivier, Annie Richard, Agnes Burel, Louis Jolly, Marc Crampon, Caroline Michel
Understanding biofilm interactions with surrounding substratum and pollutants/particles can benefit from the application of existing microscopy tools. Using the example of biofilm interactions with zero-valent iron nanoparticles (nZVI), this study aims to apply various approaches in biofilm preparation and labeling for fluorescent or electron microscopy and energy dispersive X-ray spectrometry (EDS) microanalysis for accurate observations. According to the targeted microscopy method, biofilms were sampled as flocs or attached biofilm, submitted to labeling using 4',6-diamidino-2-phenylindol, lectins PNA and ConA coupled to fluorescent dye or gold nanoparticles, and prepared for observation (fixation, cross-section, freezing, ultramicrotomy)...
November 16, 2017: Microscopy and Microanalysis
https://www.readbyqxmd.com/read/29143690/standardisation-of-the-japanese-diet-for-use-in-animal-experiments
#4
Saeko Sugawara, Yui Mizowaki, Yui Iwagaki, Yu Sakamoto, Kazushi Yamamoto, Tsuyoshi Tsuduki
The aim of this study was to develop a purified diet that mimics the characteristics of the Japanese diet using readily available materials with a simpler composition and a focus on quality, with the goal of facilitating performance of studies on the Japanese diet worldwide. The utility of the new diet was examined as a mimic of the standard Japanese diet for use in animal experiments. We examined whether a key characteristic of the Japanese diet of being less likely to cause obesity could be reproduced. The mimic diet had a balance of protein, fat and carbohydrate based on the 1975 Japanese diet, which is the least likely to cause obesity, and materials chosen with reference to the National Health and Nutrition Survey (NHNS)...
November 16, 2017: British Journal of Nutrition
https://www.readbyqxmd.com/read/29140679/a-green-approach-to-improving-the-strength-and-flame-retardancy-of-poly-vinyl-alcohol-clay-aerogels-incorporating-bio-based-gelatin
#5
Yu-Tao Wang, Hai-Bo Zhao, Kimberly C Degracia, Lin-Xuan Han, Hua Sun, Mingze Sun, Yu-Zhong Wang, David A Schiraldi
Bio-based gelatins were used to improve the compressive and flammability properties poly(vinyl alcohol)/montmorillonite (PVA/MMT) aerogels, fabricated using a simple and environmentally-friendly freeze-drying method. Due to the excellent compatibility and strong interfacial adhesion between PVA and gelatin, the compressive moduli of aerogels were enhanced dramatically with the incorporation of gelatin. PVA/MMT/porcine gelatin aerogels exhibit compressive modulus values as much as 12.4 MPa, nearly 300% that of the control PVA/MMT aerogel...
November 15, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29139545/the-effect-of-ice-crystals-formations-on-the-flesh-quality-of-frozen-horse-mackerel-trachurus-japonicus
#6
Yao Wang, Riho Miyazaki, Shinobu Saitou, Katsuya Hirasaka, Satoshi Takeshita, Katsuyasu Tachibana, Shigeto Taniyama
It is known that the formation of ice crystals has a negative impact on the flesh quality of frozen meat. This study focuses on how the formation of ice crystals in the dorsal ordinary muscle affects the flesh quality of frozen horse mackerel (Trachurus japonicus). Freeze-thawed muscle specimens (F-TMS) of horse mackerel were first stored at -24°C and then thawed. The K-value, expressible water, breaking strength of unfrozen muscle specimens (UMS) and F-TMS, and histological structure (light microscope: LM, scanning electron microscope: SEM) of UMS, frozen muscle specimens (FMS) and F-TMS were investigated...
November 15, 2017: Journal of Texture Studies
https://www.readbyqxmd.com/read/29139363/cryosurgery-for-facial-and-scalp-lesions-of-basal-cell-carcinoma-a-study-in-29-elderly-patients
#7
Satish Savant, Sandeep Savant, Virendra N Sehgal
Basal cell carcinoma (BCC) is one of the pre-eminent tumors of the epidermis, recognized by explicit clinical-pathologic characteristics. It is relatively uncommon in South Asia including India and is amenable to a variety of modalities, including cryosurgery. The outcome of cryosurgery was evaluated in 18 men and 11 women, aged 58 to 83 years, who were treated with two freeze-thaw cycles of liquid nitrogen under local anesthesia, after requisite preparations. Of the 29 patients, 27 had complete clearance of the lesions in the course of 3 years of follow-up, while only 2 had a recurrence, one at 17 months and one at 23 months...
2017: Skinmed
https://www.readbyqxmd.com/read/29139338/freeze-drying-of-silica-nanoparticles-redispersibility-toward-nanomedicine-applications
#8
Agustin S Picco, Larissa F Ferreira, Michelle S Liberato, Gabriela B Mondo, Mateus B Cardoso
AIM: To study freeze-drying of silica nanoparticles (SiO2NPs) in order to find suitable conditions to produce lyophilized powders with no aggregation after resuspension and storage. METHODS: SiO2NPs were synthesized using a Stöber-based procedure, and characterized by scanning electron microscopy, dynamic light scattering and nitrogen adsorption/desorption isotherms. SiO2NPs hydrodynamic diameters were compared prior and after freeze-drying in the presence/absence of carbohydrate protectants...
November 15, 2017: Nanomedicine
https://www.readbyqxmd.com/read/29137009/brain-metabolism-in-patients-with-freezing-of-gait-after-hypoxic-ischemic-brain-injury-a-pilot-study
#9
Seo Yeon Yoon, Sang Chul Lee, Na Young Kim, Young-Sil An, Yong Wook Kim
Movement disorders are 1 of the long-term neurological complications that can occur after hypoxic-ischemic brain injury (HIBI). However, freezing of gait (FOG) after HIBI is rare. The aim of this study was to examine the brain metabolism of patients with FOG after HIBI using F-18 fluoro-2-deoxy-D-glucose positron emission tomography (F-18 FDG PET).We consecutively enrolled 11 patients with FOG after HIBI. The patients' overall brain metabolism was measured by F-18 FDG PET, and we compared their regional brain metabolic activity with that from 15 healthy controls using a voxel-by-voxel-based statistical mapping analysis...
November 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/29135807/effects-of-stressor-controllability-on-transcriptional-levels-of-c-fos-arc-and-brain-derived-neurotrophic-factor-in-mouse-amygdala-and-medial-prefrontal-cortex
#10
Mayumi Machida, György Lonart, Larry D Sanford
Controllability is an important factor in determining stress outcomes. Uncontrollable stress is associated with the development of psychopathology such as post-traumatic stress disorder, whereas controllable stress is associated with adaptive stress responses and positive outcomes. In this study, we investigated how controllability affects poststress neurobiology by assessing transcriptional levels of activity-dependent genes in medial prefrontal cortex (mPFC) and amygdala, regions important in mediating stress outcomes...
November 13, 2017: Neuroreport
https://www.readbyqxmd.com/read/29135024/reverse-stability-kinetics-of-meat-pigment-oxidation-in-aqueous-extract-from-fresh-beef
#11
John C Frelka, David M Phinney, Macdonald P Wick, Dennis R Heldman
The use of kinetic models is an evolving approach to describing quality changes in foods during processes, including storage. Previous studies indicate that the oxidation rate of myoglobin is accelerated under frozen storage conditions, a phenomenon termed reverse stability. The goal of this study was to develop a model for meat pigment oxidation to incorporate the phenomenon of reverse stability. In this investigation, the model system was an aqueous extract from beef which was stored under a range of temperatures, both unfrozen and frozen...
November 14, 2017: Journal of Food Science
https://www.readbyqxmd.com/read/29135023/effects-of-different-pretreatments-to-fresh-fruit-on-chemical-and-thermal-characteristics-of-crude-palm-oil
#12
Minmin Tang, Qiuyu Xia, Brendan J Holland, Hui Wang, Yufeng Zhang, Rui Li, Hongxing Cao
This study selected 5 methods, including boiling, hot air drying, high-pressurized steam, freezing, and microwave radiation to pretreat fresh oil palm fruit before solvent extraction of the oil. Using fresh fruit as a control, the pretreatment methods were compared for the effects on the activity of the 2 main enzymes in the fruit and some physicochemical properties of the crude palm oil. The results indicated, although all the 5 pretreatments could inactivate lipase and peroxidase in the treated flesh significantly (P < 0...
November 14, 2017: Journal of Food Science
https://www.readbyqxmd.com/read/29134555/ciprofloxacin-loaded-calcium-alginate-wafers-prepared-by-freeze-drying-technique-for-potential-healing-of-chronic-diabetic-foot-ulcers
#13
Asif Ahmed, Giulia Getti, Joshua Boateng
Calcium alginate (CA) wafer dressings were prepared by lyophilization of hydrogels to deliver ciprofloxacin (CIP) directly to the wound site of infected diabetic foot ulcers (DFUs). The dressings were physically characterized by scanning electron microscopy (SEM), texture analysis (for mechanical and in vitro adhesion properties), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR). Further, functional properties essential for wound healing, i.e., porosity, in vitro swelling index, water absorption (Aw), equilibrium water content (EWC), water vapor transmission rate (WVTR), evaporative water loss (EWL), moisture content, in vitro drug release and kinetics, antimicrobial activity, and cell viability (MTT assay) were investigated...
November 13, 2017: Drug Delivery and Translational Research
https://www.readbyqxmd.com/read/29134300/freeze-fracture-new-avenues-for-the-ultrastructural-analysis-of-cells-in-vitro
#14
REVIEW
Carola Meier, Anja Beckmann
The ultrastructural analysis of biological membranes by freeze fracture has a 60-year tradition. In this review, we summarize the benefits of the freeze-fracture technique and review special structures analyzed by freeze fracture and by combined freeze-fracture replica immunogold labeling (FRIL) of cell cultures. In principle, every cellular membrane whether of cell suspensions, mono- or bilayers of cell cultures can be analyzed in freeze fracture. The combination of freeze fracture and immunogold labeling of the replica allows the ultrastructural identification of protein assemblies in combination with the molecular identification of their constituent proteins using specific antibodies...
November 13, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/29134193/snowball-earth-climate-dynamics-and-cryogenian-geology-geobiology
#15
Paul F Hoffman, Dorian S Abbot, Yosef Ashkenazy, Douglas I Benn, Jochen J Brocks, Phoebe A Cohen, Grant M Cox, Jessica R Creveling, Yannick Donnadieu, Douglas H Erwin, Ian J Fairchild, David Ferreira, Jason C Goodman, Galen P Halverson, Malte F Jansen, Guillaume Le Hir, Gordon D Love, Francis A Macdonald, Adam C Maloof, Camille A Partin, Gilles Ramstein, Brian E J Rose, Catherine V Rose, Peter M Sadler, Eli Tziperman, Aiko Voigt, Stephen G Warren
Geological evidence indicates that grounded ice sheets reached sea level at all latitudes during two long-lived Cryogenian (58 and ≥5 My) glaciations. Combined uranium-lead and rhenium-osmium dating suggests that the older (Sturtian) glacial onset and both terminations were globally synchronous. Geochemical data imply that CO2 was 10(2) PAL (present atmospheric level) at the younger termination, consistent with a global ice cover. Sturtian glaciation followed breakup of a tropical supercontinent, and its onset coincided with the equatorial emplacement of a large igneous province...
November 2017: Science Advances
https://www.readbyqxmd.com/read/29134152/the-role-of-mef2-transcription-factors-in-dehydration-and-anoxia-survival-in-rana-sylvatica-skeletal-muscle
#16
Myriam P Hoyeck, Hanane Hadj-Moussa, Kenneth B Storey
The wood frog (Rana sylvatica) can endure freezing of up to 65% of total body water during winter. When frozen, wood frogs enter a dormant state characterized by a cessation of vital functions (i.e., no heartbeat, blood circulation, breathing, brain activity, or movement). Wood frogs utilize various behavioural and biochemical adaptations to survive extreme freezing and component anoxia and dehydration stresses, including a global suppression of metabolic functions and gene expression. The stress-responsive myocyte enhancer factor-2 (MEF2) transcription factor family regulates the selective expression of genes involved in glucose transport, protein quality control, and phosphagen homeostasis...
2017: PeerJ
https://www.readbyqxmd.com/read/29133233/freeze-drying-from-organic-co-solvent-systems-part-1-thermal-analysis-of-co-solvent-based-placebo-formulations-in-the-frozen-state
#17
Claudia Kunz, Sonja Schuldt-Lieb, Henning Gieseler
The use of co-solvent systems has been demonstrated to shorten lengthy freeze drying processes and improve the solubility and stability of certain active pharmaceutical ingredients. The goal of the present study was to evaluate the suitability of two thermal characterization techniques, differential scanning calorimetry and freeze dry microscopy, to identify an optimal co-solvent system. Binary mixtures of a co-solvent (tert-butanol, dimethyl sulfoxide, 1,4-dioxane, acetone or ethanol) and water were investigated...
November 10, 2017: Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/29133102/development-of-bioactive-cellulose-nanocrystals-derived-from-dominant-cellulose-polymorphs-i-and-ii-from-capsosiphon-fulvescens-for-biomedical-applications
#18
Sung Won Ko, Juan Paolo E Soriano, Afeesh Rajan Unnithan, Ji Yeon Lee, Chan Hee Park, Cheol Sang Kim
Cellulose I and II polymorphs were isolated from Capsosiphon fulvescens (CF) using the conventional method of extraction and direct mercerization of raw sample, respectively. The morphological and structural differences between the isolated polymorphs were studied by FTIR, FESEM and XRD. Direct mercerization of raw CF yielded the transformation of highly crystalline cellulose I (81.3%) to II (63.7%) as observed in the shifting of XRD patterns. The derived cellulose I and II were hydrolyzed (60% w/w H2SO4, 55°C, 1hr, 10mL/g) to obtain the spindle-shaped cellulose nanocrystals...
November 10, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29132646/should-they-stay-or-should-they-go-now-exploring-the-impact-of-team-familiarity-on-interprofessional-team-training-outcomes
#19
Kavita Joshi, Jessica Hernandez, Joseph Martinez, Kareem AbdelFattah, Aimee K Gardner
INTRODUCTION: Although simulation is an effective method for enhancing team competencies, it is unclear how team familiarity impacts this process. We examined how team familiarity impacted team competencies. METHODS: Trainees were assigned to stable or dynamic teams to participate in three simulated cases. Situation awareness (SA) data was collected through in-scenario freezes. The recorded performances were assessed for clinical effectiveness (ClinEff) and teamwork...
November 4, 2017: American Journal of Surgery
https://www.readbyqxmd.com/read/29132339/sequential-e2-levels-not-ovarian-maximal-diameter-estimates-were-correlated-with-outcome-of-cetrotide-therapy-for-management-of-women-at-high-risk-of-ovarian-hyperstimulation-syndrome-a-randomized-controlled-study
#20
Khalid M Salama, Hesham M Abo Ragab, Mohammed F El Sherbiny, Ali A Morsi, Ibrahim I Souidan
BACKGROUND: Ovarian hyperstimulation syndrome (OHSS) is an important condition with considerable morbidity and a small risk of mortality and most commonly results as an iatrogenic condition following follicular stimulation of the ovaries. We aimed to evaluate safety and efficacy of 3-day cetrotide therapy started on day of oocyte retrieval (Day-0) in women at high-risk for development of ovarian hyperstimulation syndrome (OHSS) after GnRH agonist induction protocol. METHODS: Forty-eight women fulfilling inclusion criteria underwent ultrasound scanning for maximal ovarian diameter (MOD) estimation and ascites grading...
November 13, 2017: BMC Women's Health
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