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https://www.readbyqxmd.com/read/28551880/the-role-of-mkp-1-in-insulin-induced-cardioprotection
#1
Ingrid Webster, Angelique Smith, Amanda Lochner, Barbara Huisamen
PURPOSE: The mitogen-activated protein kinase phosphatases (MKPs) are a family of dual-specificity phosphatases that inactivate MAPKs by dephosphorylation. Impairment of MKP-1 expression in insulin resistance has been suggested to affect the cardioprotective properties of insulin. We hypothesized that manipulation of its activity during myocardial ischaemia/reperfusion of control as well as insulin-resistant rats may affect the outcome. METHODS: Hearts from 16 week dietary induced obese Wistar rats and their age matched controls were isolated, perfused in the working mode and subjected to 15 min global ischaemia / 30 min reperfusion or 35 min coronary artery ligation/ 60 min reperfusion...
May 27, 2017: Cardiovascular Drugs and Therapy
https://www.readbyqxmd.com/read/28551424/formulation-optimization-of-freeze-dried-long-circulating-liposomes-and-in-line-monitoring-of-the-freeze-drying-process-using-an-nir-spectroscopy-tool
#2
Bianca Sylvester, Alina Porfire, Pieter-Jan Van Bockstal, Sebastian Porav, Marcela Achim, Thomas DE Beer, Ioan Tomuță
The effect of lyoprotectant type and concentration on the stability of freeze-dried prednisolone sodium phosphate-loaded long-circulating liposomes (LCL-PLP) was investigated. Trehalose at a 5:1 carbohydrate to lipid molar ratio proved to be superior in maintaining the structural integrity and the permeability properties of the liposome bilayers, assuring the desired characteristics of the final product: a cake with a porous structure and easy to reconstitute, a similar size to the liposomes prior to freeze-drying, a high percent of encapsulated drug and a low residual moisture content...
May 24, 2017: Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28551237/impact-of-%C3%AE-irradiation-on-physicochemical-properties-of-freeze-dried-amorphophallus-paeoniifolius-flour
#3
M Suriya, C Rethina, Mudasir Bashir, Chagam Koteswara Reddy, N Harsha, Sundaramoorthy Haripriya
Freeze dried raw (FDR) and freeze dried blanched (FDB) Amorphophallus paeoniifolius flours were irradiated at doses of 3, 6 and 12kGy. The irradiated flours were investigated for physicochemical, pasting, textural, thermal and morphological properties. Reduction in amylose content was observed with increasing irradiation dose. Carboxyl content of the flours increased significantly at higher doses. Decline in lightness (L(∗)) and increase in redness (a(∗)) were found to increase with the elevation of dosage...
November 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28551225/prevention-of-denaturation-of-freshwater-crayfish-muscle-subjected-to-different-freeze-thaw-cycles-by-gelatin-hydrolysate
#4
Mehran Yasemi
The cryoprotective effect of gelatin hydrolysates from the skin of beluga sturgeon (Huso huso) on freshwater crayfish (Astacus leptodactylus) muscle subjected to different freeze-thaw cycles was investigated. Untreated muscle was particularly susceptible to quality loss as indicated by the formation of secondary lipid oxidation products and the loss in sulfhydryl groups and Ca(2+)-ATPase activity. Hydrolysate produced using flavourzyme which was mainly consisted of oligopeptides as the main fraction as well as small fraction of polypeptides could lower the denaturation of crayfish myosin heavy chain when compared to the control...
November 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28551215/effect-of-different-drying-techniques-on-bioactive-components-fatty-acid-composition-and-volatile-profile-of-robusta-coffee-beans
#5
Wenjiang Dong, Rongsuo Hu, Zhong Chu, Jianping Zhao, Lehe Tan
This study investigated the effect of different drying techniques, namely, room-temperature drying (RTD), solar drying (SD), heat-pump drying (HPD), hot-air drying (HAD), and freeze drying (FD), on bioactive components, fatty acid composition, and the volatile compound profile of robusta coffee beans. The data showed that FD was an effective method to preserve fat, organic acids, and monounsaturated fatty acids. In contrast, HAD was ideal for retaining polyunsaturated fatty acids and amino acids. Sixty-two volatile compounds were identified in the differently dried coffee beans, representing 90% of the volatile compounds...
November 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28551212/dual-crosslinked-poly-vinyl-alcohol-sodium-alginate-silver-nanocomposite-beads-a-promising-antimicrobial-material
#6
Kannan Badri Narayanan, Sung Soo Han
In this paper, we report the immobilization of borate-stabilized silver nanoparticles (AgNPs) as nanofillers in dual-crosslinked polymers comprised of poly(vinyl alcohol) (PVA) and sodium alginate (SA) at different ratios. Ionic-crosslinking using Ca(2+) ions and physical-crosslinking by freeze-thawing were used to entrap silver nanoparticles in the prepared PVA/SA/AgNPs nanocomposite beads. These polymeric nanocomposites were characterized by UV-Vis, XRD, FE-SEM, FT-IR, TGA, and using rheological and swelling properties...
November 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28551066/socially-induced-plasticity-in-sensorimotor-gating-in-the-african-cichlid-fish-astatotilapia-burtoni
#7
Heike Neumeister, Mila Adelman, William Gallagher, Jiangtao Gou, Karin Merrins, Melissa Perkowski, Stephanie Shih, Beth Terranova, Thomas Preuss
Deficits in sensorimotor gating (PPI), social defeat and social withdrawal are hallmark features of several neurological and neuropsychiatric disorders. However, the link between social environment and PPI i.e., the possible role of social defeat in driving PPI plasticity, is far from clear. Here we explored these questions in the African cichlid fish Astatotilapia burtoni, where males exist as two distinct yet reversible phenotypes. In fish communities, DOMs exhibit frequent aggressive and territorial behaviors, threatening and attacking SUBs, which respond either by engaging in fights and fleeing, or by avoiding interaction with DOMs altogether...
May 24, 2017: Behavioural Brain Research
https://www.readbyqxmd.com/read/28551040/development-of-a-candidate-stabilizing-formulation-for-bulk-storage-of-a-double-mutant-heat-labile-toxin-dmlt-protein-based-adjuvant
#8
Vishal M Toprani, Neha Sahni, John M Hickey, George A Robertson, C Russell Middaugh, Sangeeta B Joshi, David B Volkin
This work describes the formulation design and development of a novel protein based adjuvant, a double mutant of heat labile toxin (dmLT), based on knowledge of the protein's structural integrity and physicochemical degradation pathways. Various classes of pharmaceutical excipients were screened for their stabilizing effect on dmLT during exposure to thermal and agitation stresses as monitored by high throughput analytical assays for dmLT degradation. Sucrose, phosphate, sodium chloride, methionine and polysorbate-80 were identified as potential stabilizers that protected dmLT against either conformational destabilization, aggregation/particle formation or chemical degradation (e...
May 24, 2017: Vaccine
https://www.readbyqxmd.com/read/28550604/microencapsulation-of-%C3%AE-carotene-based-on-casein-guar-gum-blend-using-zeta-potential-yield-stress-phenomenon-an-approach-to-enhance-photo-stability-and-retention-of-functionality
#9
Deepika Thakur, Ashay Jain, Gargi Ghoshal, U S Shivhare, O P Katare
β-Carotene, abundant majorly in carrot, pink guava yams, spinach, kale, sweet potato, and palm oil, is an important nutrient for human health due to its scavenging action upon reactive free radicals wherever produced in the body. Inclusion of liposoluble β-carotene in foods and food ingredients is a challenging aspect due to its labile nature and low absorption from natural sources. This fact has led to the application of encapsulation of β-carotene to improve stability and bioavailability. The present work was aimed to fabricate microcapsules (MCs) of β-carotene oily dispersion using the complex coacervation technique with casein (CA) and guar gum (GG) blend...
May 26, 2017: AAPS PharmSciTech
https://www.readbyqxmd.com/read/28549919/microscopic-diffusion-in-hydrated-encysted-eggs-of-brine-shrimp
#10
E Mamontov
BACKGROUND: We have studied microscopic diffusion of water in fully hydrated encysted eggs of brine shrimp (Artemia). METHODS: We have utilized quasielastic neutron scattering. RESULTS: Dry eggs of brine shrimp were rehydrated using (1) water without additives, (2) eutectic mixture of water and dimethyl sulfoxide, and (3) a concentrated aqueous solution of lithium chloride. Despite the complexity of the hydrated multicellular organism, measurable microscopic diffusivity of water is rather well defined...
May 23, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28549549/freezing-preparation-for-macroscopic-forensic-investigation-in-putrefied-brain
#11
Kotaro Matoba, Hideki Hyodoh, Manabu Murakami, Tomoko Matoba, Atsuko Saito, Fei Feng, Shigeki Jin
PURPOSE: To evaluate the usefulness of the applied freezing technique in putrefied brain for macroscopic investigation. MATERIALS AND METHODS: From October 2015 to September 2016, first the brains of 10 cadavers (control group: male 6, female 4, age 20-80 (mean 61.5), postmortem intervals (PMI) 14-75 (mean 29.7)days) were inspected following the standard practice (without freezing preparation), and then with 10 cadavers (freezing group: male 7, female 3, age 41-88 (mean 60...
May 2017: Legal Medicine
https://www.readbyqxmd.com/read/28549168/assessing-the-thermal-dissipation-sap-flux-density-method-for-monitoring-cold-season-water-transport-in-seasonally-snow-covered-forests
#12
Allison M Chan, David R Bowling
Productivity of conifers in seasonally snow-covered forests is high before and during snowmelt when environmental conditions are optimal for photosynthesis. Climate change is altering the timing of spring in many locations, and changes in the date of transition from winter dormancy can have large impacts on annual productivity. Sap flow methods provide a promising approach to monitor tree activity during the cold season and the winter-spring and fall-winter transitions. Although sap flow techniques have been widely used, cold season results are generally not reported...
May 26, 2017: Tree Physiology
https://www.readbyqxmd.com/read/28548173/effects-of-freeze-thawing-cycles-on-desorption-behaviors-of-pah-contaminated-soil-in-the-presence-of-a-biosurfactant-a-case-study-in-western-canada
#13
Y Yao, G H Huang, C J An, G H Cheng, J Wei
Many regions in Canada are facing increasing environmental threats posed by oil and gas exploitation and transportation. These contaminated lands are inevitably subjected to seasonal and diurnal freeze-thawing cycles (FTCs). However, knowledge about the effect of FTCs on the behaviours of hydrophobic contaminants during the aging process of soil is limited. This study investigated the desorption characteristics of phenanthrene in aging soils in the presence of the biosurfactant rhamnolipid under diurnal and seasonal FTC treatments...
May 26, 2017: Environmental Science. Processes & Impacts
https://www.readbyqxmd.com/read/28547989/preservation-of-dna-nanostructure-carriers-effects-of-freeze-thawing-and-ionic-strength-during-lyophilization-and-storage
#14
Bing Zhu, Yan Zhao, Jiangbing Dai, Jianbang Wang, Shu Xing, Linjie Guo, Nan Chen, Xiangmeng Qu, Li Li, Juwen Shen, Jiye Shi, Jiang Li, Lihua Wang
DNA nanostructures have attracted wide interest in biomedical applications, especially as nanocarriers for drug delivery. Therefore, it is important to ensure the structural integrity of DNA nanostructures under ambient temperature storage. In this study, we examined lyophilization-based preservation of DNA nanostructures by investigating the structural integrity of different DNA nanostructures reconstituted from lyophilization. We demonstrated that lyophilization under appropriate ionic strength is amenable to the storage of DNA nanostructures...
May 26, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28547813/salix-arctica-changes-root-distribution-and-nutrient-uptake-in-response-to-subsurface-nutrients-in-high-arctic-deserts
#15
Amanda L Guy, Sarah P Hardy, Steven D Mamet, Mitsuaki Ota, Eric G Lamb, Steven D Siciliano
Moisture is critical for plant success in polar deserts but not by the obvious pathway of reduced water stress. We hypothesized that an indirect, nutrient-linked, pathway resulting from unique water/frozen soil interactions in polar deserts creates nutrient-rich patches critical for plant growth. These nutrient-rich patches (diapirs) form deep in high arctic polar deserts soils from water accumulating at the permafrost freezing front and ultimately rising into the upper soil horizons through cryoturbated convective landforms (frost boils)...
May 26, 2017: Ecology
https://www.readbyqxmd.com/read/28546823/study-of-guanxinning-injection-on-regulatory-mechanism-of-bcl-2-and-bax-by-liquid-nitrogen-freezing-mediated-femoral-head-necrosis
#16
Xiaofeng Zhang, Xilin Xu, Gerhard Litscher, Zemin Sheng, Lu Wang, Daniela Litscher, Hang Lv, Xiaodong Li, Jie Zhang, Hui Su, Peiran Liu, Hai Hu
The objective of this study was to investigate the impact of femoral head perfusion by traditional Chinese medicine Guanxinning injection promoting blood circulation for removing blood stasis on the expression of Bcl-2 and Bax induced by liquid nitrogen freezing-mediated femoral head necrosis. 90 rabbits were randomized into three groups. Normal control group was not subjected to any medication. Saline and Guanxinning group were perfused with 0.9% saline and Guanxinning injection once every three days through the hip joint, respectively...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/28545999/a-comparative-analysis-of-the-efficacy-of-three-cryopreservation-protocols-on-the-survival-of-in-vitro-derived-cattle-embryos-at-pronuclear-and-blastocyst-stages
#17
Van Huong Do, Simon Walton, Sally Catt, Andrew W Taylor-Robinson
The effectiveness of three cryopreservation protocols (slow freezing, short equilibration vitrification and long equilibration vitrification) on in vitro-derived cattle embryos at expanded blastocyst and pronuclear stages was compared. 199 expanded blastocysts of good quality were assigned randomly into four treatment groups [control, non-cryopreserved (fresh, unfrozen); and the three cryopreservation methods]. The re-expansion of the cryopreserved blastocysts after 24 h in vitro culture was similar to that of the fresh control group...
May 22, 2017: Cryobiology
https://www.readbyqxmd.com/read/28545986/a-simple-field-friendly-technique-for-cryopreserving-semen-from-asian-elephants-elephas-maximus
#18
Danielle M Arnold, Charlie Gray, Terri L Roth, Sebastian Mitchell, Laura H Graham
The specific objectives of the present study were to investigate the effects of manual seeding, differing freeze and thaw rates as well as storage for 24h at 4°C prior to cryopreservation on post-thaw sperm quality in Asian elephants. Extended semen was cooled in an equitainer to 4°C, frozen in liquid nitrogen vapour at various rates with and without manual seeding or in a dry shipper and thawed at 37, 50 and 75°C. There was a significant effect of freeze rate on post-thaw motility (P<0.0001) and acrosomal integrity (P<0...
May 18, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28545450/how-to-transport-veterinary-drugs-in-insulated-boxes-to-avoid-thermal-damage-by-heating-or-freezing
#19
Johannes Horak, Astrid Haberleitner, Günther Schauberger
BACKGROUND: The transport of veterinary drugs must comply with the general standards for drug storage. Although many vehicles are equipped with active heating and/or cooling devices assuring recommended storage conditions, simple insulated transport boxes are also often used. In this study, measurements for typical transport boxes were performed under laboratory conditions by the use of a climate chamber for a temperature of -20 °C and 45 °C to investigate the impact of box size, insulation material, liquid vs...
May 25, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28544951/clinical-balance-scales-indicate-worse-postural-control-in-people-with-parkinson-s-disease-who-exhibit-freezing-of-gait-compared-to-those-who-do-not-a-meta-analysis
#20
REVIEW
Esther M J Bekkers, Bauke W Dijkstra, Kim Dockx, Elke Heremans, Sabine M P Verschueren, Alice Nieuwboer
Postural instability and freezing of gait (FOG) are key features of Parkinson's disease (PD) that are closely related to falls. Uncovering the postural control differences between individuals with and without FOG contributes to our understanding of the relationship between these phenomena. The objective of this meta-analysis was to investigate whether postural control deficits, as detected by clinical balance scales, were more apparent in FOG+ compared to FOG-. Furthermore, we aimed to identify whether different scales were equally sensitive to detect postural control deficits and whether medication affected postural control differentially in each subgroup...
May 11, 2017: Gait & Posture
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