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https://www.readbyqxmd.com/read/28532053/functionalized-fe3o4-graphene-oxide-nanocomposites-with-hairpin-aptamers-for-the-separation-and-preconcentration-of-trace-pb-2-from-biological-samples-prior-to-determination-by-icp-ms
#1
Mojtaba Shamsipur, Leila Farzin, Mahmoud Amouzadeh Tabrizi, Shahab Sheibani
Lead (Pb) as a topically poisonous metal represents a serious threat to the ecological environment and especially to human beings. Therefore, it is urgent to develop a rapid and reliable monitoring technique for this heavy metal in the environmental samples. In the present study, we have designed a selective and sensitive method for the determination of ultratrace contents of Pb(2+) in biological samples, based on the guanine (G)-quadruplex formed by the aptamer with hairpin structure and Pb(2+). For this purpose, Pb(2+) specific aptamer serving as affinity probe to capture and separate trace amounts of the analyte, was covalently linked to Fe3O4/graphene oxide (GO) surface by using a suitable cross-linking agent...
August 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28532034/in-vivo-investigations-and-cytotoxicity-tests-on-ti-zr-based-metallic-glasses-with-various-cu-contents
#2
C H Lin, C H Chen, Y S Huang, C H Huang, J C Huang, J S C Jang, Y S Lin
The Ti/Zr-based metallic glasses (MGs) with various Cu contents are prepared, with nominal compositions of Ti45Zr40Si15 (Cu-free), Ti45Zr40Si10Cu5 (low-Cu), and Ti45Zr20Cu35 (high-Cu). The mechanical properties, corrosion resistance, and in-vitro biocompatibility of these MGs are investigated by means of nano-indentation, electrochemical analyses, MTS assays and inductively coupled plasma mass spectrometry, as well as in-vivo biocompatibility in terms of scanning electron microscopy, micro-CT scans and histological observations...
August 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28531844/development-of-an-uplc-ms-ms-method-for-simultaneous-quantitation-of-11-d-amino-acids-in-different-regions-of-rat-brain-application-to-a-study-on-the-associations-of-d-amino-acid-concentration-changes-and-alzheimer-s-disease
#3
Zhe Li, Yuping Xing, Xingjie Guo, Yan Cui
There are significant differences in d-amino acid concentrations between healthy people and Alzheimer's disease patients. In order to investigate the potential correlation between d-amino acids and Alzheimer's disease, a simple and sensitive ultra high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method has been developed. The method was applied to simultaneous determination of 11 d-amino acids in different regions of rat brain. Rat brain homogenates were firstly pretreated with protein precipitation procedure and then derivatized with (S)-N-(4-nitrophenoxycarbonyl) phenylalanine methoxyethyl ester [(S)-NIFE]...
May 11, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28531843/rapid-ic-ms-ms-determination-of-methylphosphonic-acid-in-urine-of-rats-exposed-to-organophosphorus-nerve-agents
#4
Timur Baygildiev, Alexandra Zatirakha, Igor Rodin, Arkady Braun, Andrey Stavrianidi, Nadezhda Koryagina, Igor Rybalchenko, Oleg Shpigun
A direct approach for the determination of a specific hydrolysis product of organophosphorus nerve agents such as methylphosphonic acid (MPA) in urine by ion chromatography and tandem mass spectrometry (IC-MS/MS) has been developed. The first advantage of the proposed approach is a rapid and simple sample preparation, which does not require a large sample volume, complicated and laborious preconcentration and derivatization steps, and takes less than 7min per sample. The second advantage is the fast and selective IC determination of MPA carried out on a noncommercial anion exchanger based on a poly(styrene-co-divinylbenzene) (PS-DVB) substrate with a high degree of crosslinking and a covalently-bonded branched functional layer, which enables complete resolution of MPA from major urine matrix components and allows one to overcome matrix effects...
May 8, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28531831/workflow-methodology-for-rat-brain-metabolome-exploration-using-nmr-lc-ms-and-gc-ms-analytical-platforms
#5
Binta Diémé, Antoine Lefèvre, Lydie Nadal-Desbarats, Laurent Galineau, Blandine Madji Hounoum, Frédéric Montigny, Hélène Blasco, Christian R Andres, Patrick Emond, Sylvie Mavel
We developed a multi-platform approach for the metabolome exploration of rat brain tissue, using liquid chromatography coupled with mass spectrometry (LC-MS), nuclear magnetic resonance spectroscopy (NMR) and gas-chromatography coupled with mass spectrometry (GC-MS). The critical steps for metabolite exploration of cerebral tissues are tissue lysis and metabolites extraction. We first evaluated the impact of freeze-drying compared to wet tissue metabolites extraction using NMR and LC-MS with a reversed phase liquid chromatography...
May 13, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28531811/synthesis-of-novel-indole-derivatives-as-promising-dna-binding-agents-and-evaluation-of-antitumor-and-antitopoisomerase-i-activities
#6
Elizabeth Almeida Lafayette, Sinara Mônica Vitalino de Almeida, Renata Virginia Cavalcanti Santos, Jamerson Ferreira de Oliveira, Cezar Augusto da Cruz Amorim, Rosali Maria Ferreira da Silva, Maira Galdino da Rocha Pitta, Ivan da Rocha Pitta, Ricardo Olimpio de Moura, Luiz Bezerra de Carvalho Júnior, Moacyr Jesus Barreto de Melo Rêgo, Maria do Carmo Alves de Lima
Molecules bearing indole nucleus present diverse biological properties such as antitumor and anti-inflammatory activities that can be associated both to DNA and protein interactions. This study focused on the synthesis of new indole derivatives with thiazolidines and imidazolidine rings condensed as side chains as well as the evaluation of their ability to interact with the DNA and antitumor and topoisomerase inhibition activities. All derivatives were successfully synthesized and their structures were elucidated by mass spectrometry (MS), infrared (IR), spectroscopy (1)H NMR, (13)C NMR, COSY (1)H-(1)H and HSQC (1)H-(13)C...
May 4, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28531138/phytochemical-composition-and-antioxidant-capacity-of-seven-saskatoon-berry-amelanchier-alnifolia-nutt-genotypes-grown-in-poland
#7
Sabina Lachowicz, Jan Oszmiański, Łukasz Seliga, Stanisław Pluta
The basic chemical composition, bioactive compounds, and antioxidant capacity of fruits of three new Polish breeding clones (No. 5/6, type S, and type N) and four Canadian cultivars (cvs.) ("Martin", "Smoky", "Pembina", and "Honeywood") grown in Poland in 2016 were investigated. Fruits were analyzed for their contents of triterpenoids, carotenoids, chlorophylls, and polyphenolics with the ultra-performance liquid chromatography photodiode detector-quadrupole/time-of-flight mass spectrometry (UPLC-PDA-Q/TOF-MS) method, sugar with the high-performance liquid chromatography-evaporative light scattering detector (HPLC-ELSD) method, and antioxidant capacity with the ability to reduce free radical (ABTS) and ferric reducing ability of plasma (FRAP) method...
May 21, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28530825/effect-of-wood-aging-on-wine-mineral-composition-and-87sr-86sr-isotopic-ratio
#8
Ayse Deniz Kaya, Raúl Bruno de Sousa, António Sérgio Curvelo-Garcia, Jorge Manuel Ricardo-da-Silva, Sofia Catarino
Evolution of mineral composition and wine strontium isotopic ratio 87Sr/86Sr (Sr IR) during wood aging were investigated. A red wine was aged in stainless steel tanks with French oak staves (Quercus sessiliflora Salisb.), with three industrial scale replicates. Sampling was carried out after 30, 60 and 90 days of aging, and the wines evaluated in terms of general analysis, phenolic composition, total polysaccharides, multi-element composition and Sr IR. Li, Be, Mg, Al, Sc, Ti, V, Mn, Co, Ni, Cu, Zn, Ga, Ge, As, Rb, Sr, Y, Zr, Mo, Sb, Cs, Ba, Pr, Nd, Sm, Eu, Dy, Ho, Er, Yb, Lu, Tl, Pb elements and 87Sr/86Sr were determined by quadrupole inductively coupled plasma mass spectrometry (Q-ICP-MS), Na, K, Ca and Fe by flame atomic absorption spectrometry (FAAS)...
May 22, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28530810/seleniranium-ions-undergo-%C3%AF-ligand-exchange-via-an-associative-mechanism-in-the-gas-phase
#9
Shea Fern Lim, Benjamin Leigh Harris, George N Khairallah, Evan John Bieske, Philippe Maître, Gabriel da Silva, Brian D Adamson, Michael S Scholz, Neville J A Coughlan, Richard A J O'Hair, Michael Rathjen, Daniel Stares, Jonathan M White
Collision-induced dissociation mass spectrometry of the ammonium ions 4a and 4b results in the formation of the seleniranium ion 5, the structure and purity of which was verified using gas phase infra-red spectroscopy coupled to mass spectrometry, and gas phase ion-mobility measurements. Ion-molecule reactions between the ion 5 (m/z = 261) and cyclopentene, cyclohexene, cycloheptene and cyclooctene, resulted in the formation of the seleniranium ions 7 (m/z = 225), 6 (m/z = 239), 8 (m/z = 253), and 9 (m/z = 267) respectively...
May 22, 2017: Journal of Organic Chemistry
https://www.readbyqxmd.com/read/28530703/trpv4-plays-a-role-in-breast-cancer-cell-migration-via-ca-2-dependent-activation-of-akt-and-downregulation-of-e-cadherin-cell-cortex-protein
#10
W H Lee, L Y Choong, T H Jin, N N Mon, S Chong, C S Liew, T Putti, S Y Lu, C Harteneck, Y P Lim
TRPV4 belongs to the 'Transient Receptor Potential' (TRP) superfamily. It has been identified to profoundly affect a variety of physiological processes, including nociception, heat sensation and inflammation. Unlike other TRP superfamily channels, its role in cancers are unknown until recently when we reported TRPV4 to be required for cancer cell softness that may promote breast cancer cell extravasation and metastasis. Here, we elucidated the molecular mechanisms mediated by TRPV4 in the metastatic breast cancer cells...
May 22, 2017: Oncogenesis
https://www.readbyqxmd.com/read/28530613/combination-of-solvent-extractants-for-dispersive-liquid-liquid-microextraction-of-fungicides-from-water-and-fruit-samples-by-liquid-chromatography-with-tandem-mass-spectrometry
#11
Marta Pastor-Belda, Isabel Garrido, Natalia Campillo, Pilar Viñas, Pilar Hellín, Pilar Flores, José Fenoll
A multiresidue method was developed to determine twenty-five fungicides belonging to three different chemical families, oxazoles, strobilurins and triazoles, in water and fruit samples, using dispersive liquid-liquid microextraction (DLLME) and liquid chromatography/tandem mass spectrometry (LC-MS(2)). Solid-liquid extraction with acetonitrile was used for the analysis in fruits, the extract being used as dispersant solvent in DLLME. Since some of the analytes showed high affinity for chloroform and the others were more efficiently extracted with undecanol, a mixture of both solvents was used as extractant in DLLME...
October 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28530588/capillary-electrophoresis-coupled-to-mass-spectrometry-employing-hexafluoro-2-propanol-for-the-determination-of-nucleosides-and-nucleotide-mono-di-and-tri-phosphates-in-baby-foods
#12
María Mateos-Vivas, Javier Domínguez-Álvarez, Encarnación Rodríguez-Gonzalo, Rita Carabias-Martínez
The present work describes a method for the simultaneous determination of unmodified nucleosides and nucleotide mono-, di- and tri-phosphates by capillary electrophoresis coupled to mass spectrometry (CE-MS). The use of hexafluoro-2-propanol (HFIP) in the separation medium, and as an additive to the sheath liquid of the electrospray interface (ESI), generated a highly efficient and sensitive method. Instrumental limits of detection in the range of 14-53ngmL(-1) for nucleosides and 7-23, 20-49 and 64-124ngmL(-1) for nucleotide mono-, di-, and tri-phosphates, respectively, were found...
October 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28530587/trace-elements-in-raw-milk-of-buffaloes-bubalus-bubalis-from-campania-italy
#13
Mauro Esposito, Oto Miedico, Stefania Cavallo, Roberta Pellicanò, Guido Rosato, Loredana Baldi, A Eugenio Chiaravalle
The profile of 18 trace elements was traced in 68 milk samples collected from buffalo farms in the territory known as the "Land of Fires" in the Campania region (Italy). This area has been polluted by the illegal dumping in fields of industrial or domestic waste, wich is sometimes then burned spreading toxic contaminants. Milk from buffaloes raised on rural farms might be a good indicator of environmental contamination risk in the human food chain. Trace element analysis in milk was performed using mass spectrometry...
October 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28530584/triacylglycerol-regioisomers-in-human-milk-resolved-with-an-algorithmic-novel-electrospray-ionization-tandem-mass-spectrometry-method
#14
Heikki Kallio, Matts Nylund, Pontus Boström, Baoru Yang
A highly sensitive mass spectrometric (MS) method was developed and validated to analyze ratios of regioisomeric triacylglycerols (TAGs) in fats and oils. UPLC resolution of lithiated TAGs followed by daughter scan MS/MS of positive ions revealed several indicative ions for quantitative analysis. Reference TAGs containing C14-C20 fatty acids (FAs) showed good linear response. Analysis of Finnish and Chinese pooled human milk samples revealed hundreds of regioisomeric TAGs. At least 64mol% of the TAGs were quantified with relative standard deviation <17%...
October 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28530582/proton-transfer-reaction-mass-spectrometry-ptr-ms-for-the-authentication-of-regionally-unique-south-african-lamb
#15
Sara W Erasmus, Magdalena Muller, Martin Alewijn, Alex H Koot, Saskia M van Ruth, Louwrens C Hoffman
The volatile fingerprints of South African lamb meat and fat were measured by proton-transfer mass spectrometry (PTR-MS) to evaluate it as an authentication tool. Meat and fat of the Longissimus lumborum (LL) of lambs from six different regions were assessed. Analysis showed that the volatile fingerprints were affected by the origin of the meat. The classification of the origin of the lamb was achieved by examining the calculated and recorded fingerprints in combination with chemometrics. Four different partial least squares discriminant analysis (PLS-DA) models were fitted to the data to classify lamb meat and fat samples into "region of origin" (six different regions) and "origin" (Karoo vs...
October 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28530580/offgel-electrophoresis-and-tandem-mass-spectrometry-approach-compared-with-dna-based-pcr-method-for-authentication-of-meat-species-from-raw-and-cooked-ground-meat-mixtures-containing-cattle-meat-water-buffalo-meat-and-sheep-meat
#16
Basappa M Naveena, Deepak S Jagadeesh, A Jagadeesh Babu, T Madhava Rao, Veeranna Kamuni, S Vaithiyanathan, Vinayak V Kulkarni, Srikanth Rapole
The present study compared the accuracy of an OFFGEL electrophoresis and tandem mass spectrometry-based proteomic approach with a DNA-based method for meat species identification from raw and cooked ground meat mixes containing cattle, water buffalo and sheep meat. The proteomic approach involved the separation of myofibrillar proteins using OFFGEL electrophoresis, SDS-PAGE and protein identification by MALDI-TOF MS. Species-specific peptides derived from myosin light chain-1 and 2 were identified for authenticating buffalo meat spiked at a minimum 0...
October 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28530578/determination-of-trace-patulin-in-apple-based-food-matrices
#17
REVIEW
Xianjiang Li, Hongmei Li, Xiaomin Li, Qinghe Zhang
Patulin is a toxic metabolite of a number of fungi; its toxicity is serious and its contamination in food is a worldwide problem, especially in apple-based food. Effective control of patulin contamination strongly depends on reliable analytical methods. In this review, various analytical methods, especially those that have appeared in the last ten years, are summarized, including the highly reproducible chromatography and mass-spectrometry-based methods, highly selective sensor-based methods and indirect quantitative PCR methods...
October 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28530554/simultaneous-determination-of-fumonisins-b1-and-b2-in-different-types-of-maize-by-matrix-solid-phase-dispersion-and-hplc-ms-ms
#18
Gabriel Barros de Oliveira, Carolyne Menezes de Castro Gomes Vieira, Ricardo Mathias Orlando, Adriana Ferreira Faria
This work involved the optimization and validation of a method, according to Directive 2002/657/EC and the Analytical Quality Assurance Manual of Ministério da Agricultura, Pecuária e Abastecimento, Brazil, for simultaneous extraction and determination of fumonisins B1 and B2 in maize. The extraction procedure was based on a matrix solid phase dispersion approach, the optimization of which employed a sequence of different factorial designs. A liquid chromatography-tandem mass spectrometry method was developed for determining these analytes using the selected reaction monitoring mode...
October 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28530406/comparative-evaluation-of-small-molecular-additives-and-their-effects-on-peptide-protein-identification
#19
Jing Gao, Shaoyun Zhong, Yanting Zhou, Han He, Shuying Peng, Zhenyun Zhu, Xing Liu, Jing Zheng, Bin Xu, Hu Zhou
Detergents and salts are widely used in lysis buffers to enhance protein extraction from biological samples, facilitating in-depth proteomic analysis. However, these detergents and salt additives must be efficiently removed from the digested samples prior to LC-MS/MS analysis to obtain high-quality mass spectra. Although filter-aided sample preparation (FASP), acetone precipitation (AP), followed by in-solution digestion, and strong cation exchange-based centrifugal proteomic reactors (CPRs) are commonly used for proteomic sample processing, little is known about their efficiencies at removing detergents and salt additives...
May 22, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28530388/microfluidic-capillary-electrophoresis-mass-spectrometry-for-analysis-of-monosaccharides-oligosaccharides-and-glycopeptides
#20
Kshitij Khatri, Joshua A Klein, John Haserick, Deborah R Leon, Catherine E Costello, Mark E McComb, Joseph Zaia
Glycomics and glycoproteomics analyses by mass spectrometry require efficient front-end separation methods to enable deep characterization of heterogeneous glycoform populations. Chromatography methods are generally limited in their ability to resolve glycoforms using mobile phases that are compatible with online liquid chromatography-mass spectrometry (LC-MS). The adoption of capillary electrophoresis-mass spectrometry methods (CE-MS) for glycomics and glycoproteomics is limited by the lack of convenient interfaces for coupling the CE devices to mass spectrometers...
May 22, 2017: Analytical Chemistry
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