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https://www.readbyqxmd.com/read/28520506/urinary-nanovesicles-captured-by-lectins-or-antibodies-demonstrate-variations-in-size-and-surface-glycosylation-profile
#1
Jared Q Gerlach, Ciaran M Maguire, Anja Krüger, Lokesh Joshi, Adriele Prina-Mello, Matthew D Griffin
AIM: The use of carbohydrate-binding proteins (lectins) to isolate urinary extracellular vesicles (uEVs) was investigated and the captured subpopulations were characterized. METHODS: Pooled uEVs from multiple healthy donors were exposed to lectin-conjugated or antibody-conjugated beads. Recovered uEVs were evaluated by protein estimation, transmission electron microscopy, nanoparticle tracking analysis and lectin microarray profiling. RESULTS: uEVs isolated by lectin- and antibody-based affinity capture exhibited distinct variations in size and surface content...
May 18, 2017: Nanomedicine
https://www.readbyqxmd.com/read/28490038/pediatric-sleep-disordered-breathing-an-update-on-diagnostic-testing
#2
Brian McGrath, Jerrold Lerman
PURPOSE OF REVIEW: Recent advances in diagnostic testing for obstructive sleep apnea in children have refined the standard tests while identifying several new tools that hold promise to radically change how we diagnose sleep apnea. RECENT FINDINGS: Studies have demonstrated that the polysomnogram may be modified to permit home assessment of sleep disturbed breathing in children to ensure more widespread access to the test. Alternately, questionnaires, nocturnal oximetry, and diagnostic urinary biomarkers have shown great promise as both sensitive and specific tools to diagnose sleep apnea in children as well as track the severity of the disease...
June 2017: Current Opinion in Anaesthesiology
https://www.readbyqxmd.com/read/28415542/bladder-smooth-muscle-cells-on-electrospun-poly-%C3%AE%C2%B5-caprolactone-poly-l-lactic-acid-scaffold-promote-bladder-regeneration-in-a-canine-model
#3
Nasser Shakhssalim, Masoud Soleimani, Mohammad M Dehghan, Javad Rasouli, Masoud Taghizadeh-Jahed, Peyman M Torbati, Mohammad Naji
Engineering of urinary bladder has been the focus of numerous studies in recent decade. Novel biomaterials, innovative fabrication methods and various modification processes of scaffolds are the critical issues to find supportive matrices. Supportive characteristics of electrospun PCL/PLLA nano-scaffold for bladder augmentation in canine model and the role of bladder cells in regeneration process were appraised. Electrospun PCL/PLLA was fabricated by co-electrospinning of PCL and PLLA. Bladder cells were isolated and transduced with lentiviral particles encoding eGFP and JRed proteins...
June 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28412047/patient-reported-quality-of-life-after-proton-beam-therapy-for-prostate-cancer-the-effect-of%C3%A2-prostate-size
#4
Anuj Goenka, Neil B Newman, Hiral Fontanilla, Oren Cahlon, Brian Chon, Henry Tsai, Eugen Hug, Carl Brown, Carlos Vargas, Rahul R Parikh
BACKGROUND: In the present study, we assessed the effect of prostate gland size on patient-assessed genitourinary and gastrointestinal (GI) quality of life (QOL) after definitive treatment of prostate adenocarcinoma with proton beam therapy. PATIENTS AND METHODS: As a part of a prospective outcome tracking protocol, 81 patients treated at a single center between with proton beam therapy completed the Expanded Prostate Cancer Index Composite (EPIC) questionnaire before treatment and at the follow-up examinations...
March 18, 2017: Clinical Genitourinary Cancer
https://www.readbyqxmd.com/read/28406764/the-efficacy-of-tocilizumab-for-the-treatment-of-chinese-takayasu-s-arteritis
#5
Jiaxin Zhou, Zhe Chen, Jing Li, Yunjiao Yang, Jiuliang Zhao, Hu Chen, Min Liu, Fei Sun, Mengtao Li, Xinping Tian, Xiaofeng Zeng
OBJECTIVES: The aim of this study was to evaluate the efficacy and safety of tocilizumab (TCZ) in Chinese Takayasu's arteritis (TAK) patients. METHODS: This was a single-centre prospective study. Sixteen consecutive TAK patients were included. Patients were treated with tocilizumab infusions with a dosage of 8 mg/kg. Serum inflammation markers including erythrocyte sedimentation rate (ESR) and hypersensitivity C-reactive protein (hsCRP) were recorded at baseline and before each TCZ infusion...
March 2017: Clinical and Experimental Rheumatology
https://www.readbyqxmd.com/read/28357510/tracking-unnecessary-negative-urinalyses-to-reduce-healthcare-costs-a-transversal-study
#6
A Malmartel, M Dutron, C Ghasarossian
About 7 million urinalyses are reimbursed yearly by the French public healthcare system, but the results of most of these tests are normal. The aim of this study was to estimate the prevalence of negative urinalyses in ambulatory care, identify the associated factors and assess the relevance of prescriptions by general practitioners (GPs) according to French guidelines. A cross-sectional study was conducted in patients over 18 coming for urinalyses in two French ambulatory laboratories. Patients received a questionnaire on their symptoms, the reason for performing urinalysis and the use of urinary dipsticks...
March 29, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28341427/community-onset-carbapenem-resistant-klebsiella-pneumoniae-urinary-tract-infections-in-infancy-following-nicu-hospitalisation
#7
Eleni Vergadi, Maria Bitsori, Sofia Maraki, Emmanouil Galanakis
INTRODUCTION: Urinary tract infection (UTI) is a common bacterial infection in childhood with favourable outcome. However, the recent emergence of UTI caused by multidrug-resistant pathogens, such as carbapenem-resistant Enterobacteriaceae (CRE), has become a great concern worldwide. CRE are mainly responsible for nosocomial infections and community-onset CRE infections in healthy individuals are rare. OBJECTIVES: In this study, we report a series of infants without substantial genitourinary abnormalities that were admitted with community-onset urinary tract infections (UTIs) caused by carbapenem-resistant Klebsiella pneumoniae (CRKP) and we discuss their aetiology...
March 11, 2017: Journal of Pediatric Urology
https://www.readbyqxmd.com/read/28322213/chronic-monitoring-of-lower-urinary-tract-activity-via-a-sacral-dorsal-root-ganglia-interface
#8
Abeer Khurram, Shani E Ross, Zachariah J Sperry, Aileen Ouyang, Christopher Stephan, Ahmad A Jiman, Tim M Bruns
OBJECTIVE: Our goal is to develop an interface that integrates chronic monitoring of lower urinary tract (LUT) activity with stimulation of peripheral pathways. APPROACH: Penetrating microelectrodes were implanted in sacral dorsal root ganglia (DRG) of adult male felines. Peripheral electrodes were placed on or in the pudendal nerve, bladder neck and near the external urethral sphincter. Supra-pubic bladder catheters were implanted for saline infusion and pressure monitoring...
March 21, 2017: Journal of Neural Engineering
https://www.readbyqxmd.com/read/28303635/incident-microalbuminuria-and-complement-factor-mannan-binding-lectin-associated-protein-19-in-people-with-newly-diagnosed-type-1-diabetes
#9
J A Østergaard, S Thiel, I T Hoffmann-Petersen, P Hovind, H-H Parving, L Tarnow, P Rossing, T K Hansen
BACKGROUND: Evidence links the lectin pathway of complement activation to diabetic kidney disease. Upon carbohydrate-recognition by pattern-recognition molecules, eg, mannan-binding lectin (MBL), the MBL-associated serine protease (MASP-2) is activated and initiates the complement cascade. The MASP2 gene encodes MASP-2 and the alternative splice product MBL-associated protein 19 (MAp19). Both MAp19 and MASP-2 circulate in complex with MBL. We tested the hypothesis that MAp19 and MASP-2 concentrations predict the risk of incident microalbuminuria...
March 16, 2017: Diabetes/metabolism Research and Reviews
https://www.readbyqxmd.com/read/28302674/urine-culture-follow-up-and-antimicrobial-stewardship-in-a-pediatric-urgent-care-network
#10
Dipanwita Saha, Jimisha Patel, Don Buckingham, David Thornton, Terry Barber, Joshua R Watson
BACKGROUND AND OBJECTIVES: Empiric antibiotic therapy for presumed urinary tract infection (UTI) leads to unnecessary antibiotic exposure in many children whose urine culture results fail to confirm the diagnosis. The objective of this quality improvement study was to improve follow-up management of negative urine culture results in the off-campus urgent care network of Nationwide Children's Hospital to reduce inappropriate antibiotic exposure in children. METHODS: A multidisciplinary task force developed and implemented a protocol for routine nurse and clinician follow-up of urine culture results, discontinuation of unnecessary antibiotics, and documentation in the electronic medical record...
March 16, 2017: Pediatrics
https://www.readbyqxmd.com/read/28300033/-current-status-of-extracorporeal-shock-wave-lithotripsy-in-urinary-lithiasis
#11
Jose Gregorio Pereira-Arias, Mikel Gamarra-Quintanilla, Luis Felipe Urdaneta-Salegui, Jorge Alberto Mora-Christian, Andrea Sánchez-Vazquez, Ander Astobieta-Odriozola, Gaspar Ibarluzea-González
Over the last decade, urinary lithiasis' prevalence has dramatically increased due to diet and lifestyle changes, growing 10.6% and 7.1% in men and women respectively. Extracorporeal shock wave lithotripsy has lost relevance in current practice due to endoscopic device development and unpredictability of results. Instrument miniaturization is leading to an increase of the percutaneous approach of increasingly smaller stones, while most flexible ureteroscopes durability and digitalization has allowed urologists to address larger stones...
March 2017: Archivos Españoles de Urología
https://www.readbyqxmd.com/read/28290186/phosphorus-and-other-aspects-of-ckd-mbd-in-the-conservative-management-of-chronic-kidney-disease
#12
Anuja Shah
As the prevalence of chronic kidney disease (CKD) increases and the population ages, there is an imperative to offer cost effective and patient specific therapeutic options for the management of advanced CKD. In cases where there is a desire to avoid or delay renal replacement therapy, conservative options need to be defined and strategies for delaying the need for renal replacement therapy should be offered. CKD-mineral bone disorders (MBD) refers to the constellation of disturbances in abnormal bone and soft tissue calcification along with abnormalities, in phosphorus, calcium, parathyroid hormone, vitamin D, and FGF-23...
June 2017: Panminerva Medica
https://www.readbyqxmd.com/read/28151401/left-ventricular-structure-and-function-in-relation-to-environmental-exposure-to-lead-and-cadmium
#13
Wen-Yi Yang, Zhen-Yu Zhang, Lutgarde Thijs, Nicholas Cauwenberghs, Fang-Fei Wei, Lotte Jacobs, Aernout Luttun, Peter Verhamme, Tatiana Kuznetsova, Tim S Nawrot, Jan A Staessen
BACKGROUND: Experimental studies have demonstrated that lead and cadmium have direct toxic effects on the myocardium, but the few human studies are limited by design, assessment of exposure, and use of heart failure as a late-stage endpoint. METHODS AND RESULTS: In a prospective population study, we studied the association of left ventricular (LV) function with blood lead (BPb) and 24-hour urinary cadmium (UCd). In 179 participants randomly recruited from a Flemish population (50...
February 1, 2017: Journal of the American Heart Association
https://www.readbyqxmd.com/read/28143592/tracking-platyhelminth-parasite-diversity-from-freshwater-turtles-in-french-guiana-first-report-of-neopolystoma-price-1939%C3%A2-monogenea-polystomatidae-with-the-description-of-three-new-species
#14
Louis H Du Preez, Mathieu Badets, Laurent Héritier, Olivier Verneau
BACKGROUND: Polystomatid flatworms in chelonians are divided into three genera, i.e. Polystomoides Ward, 1917, Polystomoidella Price, 1939 and Neopolystoma Price, 1939, according to the number of haptoral hooks. Among the about 55 polystome species that are known to date from the 327 modern living chelonians, only four species of Polystomoides are currently recognised within the 45 South American freshwater turtles. METHODS: During 2012, several sites in the vicinity of the cities Cayenne and Kaw in French Guiana were investigated for freshwater turtles...
January 31, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28138432/spontaneous-nephrocutaneous-fistula-rare-complication-of-xanthogranulomatous-pyelonephritis
#15
Rami Alazab, Hamzeh M Ghawanmeh, Faris Abushamma, Omar Ababneh, Anas I Al-Karasneh
Renocutaneous fistulae may occur as a result of chronic infection, especially in the setting of calculous disease.(1) Spontaneous renocutaneous is rare.(2) Usually nephrocutanous fistula developed in patient with previous renal surgery, trauma, tumors, and chronic urinary tract infection with abscess formation. We report a case of spontaneous nephrocutaneous fistula. A 37-year-old women previously well presented to ER department with purulent discharge and a palpable mass on the left lumbar area. CT scan with IV contrast fistulous tract seen within the subcutaneous plane in Left lumbar region tracking towards retroperitoneal space, and in continuity with of left kidney...
February 2017: Urology Case Reports
https://www.readbyqxmd.com/read/28114076/a-multi-technique-reconfigurable-electrochemical-biosensor-enabling-personal-health-monitoring-in-mobile-devices
#16
Alexander Sun, A G Venkatesh, Drew A Hall
This paper describes the design and characterization of a reconfigurable, multi-technique electrochemical biosensor designed for direct integration into smartphone and wearable technologies to enable remote and accurate personal health monitoring. By repurposing components from one mode to the next, the biosensor's potentiostat is able reconfigure itself into three different measurements modes to perform amperometric, potentiometric, and impedance spectroscopic tests all with minimal redundant devices. A [Formula: see text] PCB prototype of the module was developed with discrete components and tested using Google's Project Ara modular smartphone...
September 26, 2016: IEEE Transactions on Biomedical Circuits and Systems
https://www.readbyqxmd.com/read/28113176/a-multi-technique-reconfigurable-electrochemical-biosensor-enabling-personal-health-monitoring-in-mobile-devices
#17
Alexander Sun, A G Venkatesh, Drew A Hall
This paper describes the design and characterization of a reconfigurable, multi-technique electrochemical biosensor designed for direct integration into smartphone and wearable technologies to enable remote and accurate personal health monitoring. By repurposing components from one mode to the next, the biosensor's potentiostat is able reconfigure itself into three different measurements modes to perform amperometric, potentiometric, and impedance spectroscopic tests all with minimal redundant devices. A [Formula: see text] PCB prototype of the module was developed with discrete components and tested using Google's Project Ara modular smartphone...
October 2016: IEEE Transactions on Biomedical Circuits and Systems
https://www.readbyqxmd.com/read/28074019/point-organ-radiation-dose-in-abdominal-ct-effect-of-patient-off-centering-in-an-experimental-human-cadaver-study
#18
Ranish Deedar Ali Khawaja, Sarabjeet Singh, Atul Padole, Alexi Otrakji, Diego Lira, Da Zhang, Bob Liu, Andrew Primak, George Xu, Mannudeep K Kalra
To determine the effect of patient off-centering on point organ radiation dose measurements in a human cadaver scanned with routine abdominal CT protocol. A human cadaver (88 years, body-mass-index 20 kg/m(2)) was scanned with routine abdominal CT protocol on 128-slice dual source MDCT (Definition Flash, Siemens). A total of 18 scans were performed using two scan protocols (a) 120 kV-200 mAs fixed-mA (CTDIvol 14 mGy) (b) 120 kV-125 ref mAs (7 mGy) with automatic exposure control (AEC, CareDose 4D) at three different positions (a) gantry isocenter, (b) upward off-centering and (c) downward off-centering...
January 10, 2017: Radiation Protection Dosimetry
https://www.readbyqxmd.com/read/28068467/exodisc-for-rapid-size-selective-and-efficient-isolation-and-analysis-of-nanoscale-extracellular-vesicles-from-biological-samples
#19
Hyun-Kyung Woo, Vijaya Sunkara, Juhee Park, Tae-Hyeong Kim, Ja-Ryoung Han, Chi-Ju Kim, Hyun-Il Choi, Yoon-Keun Kim, Yoon-Kyoung Cho
Extracellular vesicles (EVs) are cell-derived, nanoscale vesicles that carry nucleic acids and proteins from their cells of origin and show great potential as biomarkers for many diseases, including cancer. Efficient isolation and detection methods are prerequisites for exploiting their use in clinical settings and understanding their physiological functions. Here, we presented a rapid, label-free, and highly sensitive method for EV isolation and quantification using a lab-on-a-disc integrated with two nanofilters (Exodisc)...
February 28, 2017: ACS Nano
https://www.readbyqxmd.com/read/28009729/impact-of-enhanced-recovery-after-surgery-and-fast-track-surgery-pathways-on-healthcare-associated-infections-results-from-a-systematic-review-and-meta-analysis
#20
Michael C Grant, Dongjie Yang, Christopher L Wu, Martin A Makary, Elizabeth C Wick
OBJECTIVE: The aim of this study was to establish if enhanced recovery after surgery (ERAS) and fast track surgery (FTS) protocols are associated with reduction in healthcare-associated infection (HAIs). BACKGROUND: Evidence suggests that prevention strategies for HAIs should be multifaceted and transdisciplinary. ERAS and FTS protocols are collaborative approaches to perioperative care which reduce length of stay but may also be an effective strategy for reducing HAIs...
January 2017: Annals of Surgery
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