Read by QxMD icon Read

"Directional migration"

Nisarat Ruangsawasdi, Matthias Zehnder, Raphael Patcas, Chafik Ghayor, Barbara Siegenthaler, Bebeka Gjoksi, Franz E Weber
Conventional root canal treatment in immature permanent teeth can lead to early tooth loss in children because root formation is discontinued. We investigated whether the stem cell factor (SCF) could facilitate cell homing in the pulpless immature root canal and promote regeneration of a functional pulp. In vitro, human mesenchymal stem cells (hMSCs) were exposed to SCF at various concentrations for assessing cell migration, proliferation, and differentiation towards odonto/osteoblasts by 3D-chemotaxis slides, WST-1 assay, and alkaline phosphatase activity, respectively...
October 20, 2016: Tissue Engineering. Part A
Kerstin Kick, Katharina Nekolla, Markus Rehberg, Angelika M Vollmar, Stefan Zahler
OBJECTIVE: Cell-matrix interactions are crucial for regulating cellular activities, such as migration. This is of special importance for morphogenic processes, such as angiogenesis (the development of new blood vessels). Most of our understanding of cell migration relies on 2-dimensional (2D) experiments. However, the awareness that 3D settings might elicit different results has increased. Knowledge about endothelial cell (EC) behavior in 3D environments and the influence of matrix composition on EC migration, in particular, is still limited...
October 13, 2016: Arteriosclerosis, Thrombosis, and Vascular Biology
Huabin Zhang, Jing Wei, Juncai Dong, Guigao Liu, Li Shi, Pengfei An, Guixia Zhao, Jintao Kong, Xiaojun Wang, Xianguang Meng, Jing Zhang, Jinhua Ye
Modular optimization of metal-organic frameworks (MOFs) was realized by incorporation of coordinatively unsaturated single atoms in a MOF matrix. The newly developed MOF can selectively capture and photoreduce CO2 with high efficiency under visible-light irradiation. Mechanistic investigation reveals that the presence of single Co atoms in the MOF can greatly boost the electron-hole separation efficiency in porphyrin units. Directional migration of photogenerated excitons from porphyrin to catalytic Co centers was witnessed, thereby achieving supply of long-lived electrons for the reduction of CO2 molecules adsorbed on Co centers...
October 13, 2016: Angewandte Chemie
Shan Yu, Ying Gao, Xu Mei, Tanchen Ren, Su Liang, Zhengwei Mao, Changyou Gao
The selective adhesion and migration of endothelial cells (ECs) over smooth muscle cells (SMCs) is very important in the rapid endothelialization of blood-contacting implants, to prevent vascular restenosis. In this study, a uniform cell-resistant layer of methacrylate-functionalized hyaluronic acid (HA) was first immobilized on a poly(ε-caprolactone) (PCL) film via polydopamine coupling. Then, a density gradient of thiol-functionalized Arg-Glu-Asp-Val (REDV) peptide was prepared on the HA layer via thiol-ene click chemistry and the continuous injection method...
October 10, 2016: ACS Applied Materials & Interfaces
Xiuling Li, Liang Ma, Dayong Wang, Xiao Cheng Zeng, Xiaojun Wu, Jinlong Yang
Extended line defects in two-dimensional (2D) materials can play an important role in modulating their electronic properties. During the experimental synthesis of 2D materials, line defects are commonly generated at grain boundaries between domains of different orientations. In this work, twelve types of line-defect structures in single crystalline phosphorene are examined by using first-principles calculations. These line defects are typically formed via migration and aggregation of intrinsic point defects, including the Stone-Wales (SW), single or double vacancy (SV or DV) defects...
October 6, 2016: Nanoscale
Jane K Rebman, Kathryn E Kirchoff, Gregory S Walsh
Collective migration depends on cell-cell interactions between neighbors that contribute to their overall directionality, yet the mechanisms that control the coordinated migration of neurons remains to be elucidated. During hindbrain development, facial branchiomotor neurons (FBMNs) undergo a stereotypic tangential caudal migration from their place of birth in rhombomere (r)4 to their final location in r6/7. FBMNs engage in collective cell migration that depends on neuron-to-neuron interactions to facilitate caudal directionality...
2016: PloS One
Tatjana J Autenrieth, Stephanie C Frank, Alexandra M Greiner, Dominik Klumpp, Benjamin Richter, Mario Hauser, Seong-Il Lee, Joel Levine, Martin Bastmeyer
Although much is known about chemotaxis- induced by gradients of soluble chemical cues - the molecular mechanisms involved in haptotaxis (migration induced by substrate-bound protein gradients) are largely unknown. We used micropatterning to produce discontinuous gradients consisting of μm-sized fibronectin-dots arranged at constant lateral but continuously decreasing axial spacing. Parameters like gradient slope, protein concentration and size or shape of the fibronectin dots were modified to determine optimal conditions for directional cell migration in gradient patterns...
October 10, 2016: Integrative Biology: Quantitative Biosciences From Nano to Macro
Weiyue Zheng, Masataka Umitsu, Ishaan Jagan, Charles W Tran, Noboru Ishiyama, Michael BeGora, Kiyomi Araki, Pamela S Ohashi, Mitsuhiko Ikura, Senthil K Muthuswamy
The polarity protein Scribble (SCRIB) regulates apical-basal polarity, directional migration and tumour suppression in Drosophila and mammals. Here we report that SCRIB is an important regulator of myeloid cell functions including bacterial infection and inflammation. SCRIB interacts directly with the NADPH oxidase (NOX) complex in a PSD95/Dlg/ZO-1 (PDZ)-domain-dependent manner and is required for NOX-induced reactive oxygen species (ROS) generation in culture and in vivo. On bacterial infection, SCRIB localized to phagosomes in a leucine-rich repeat-dependent manner and promoted ROS production within phagosomes to kill bacteria...
October 3, 2016: Nature Cell Biology
Samina Arshid, Muhammad Tahir, Belchor Fontes, Edna Frasson de Souza Montero, Mariana Souza Castro, Simone Sidoli, Peter Roepstorff, Wagner Fontes
In clinical conditions trauma is associated with high mortality and morbidity. Neutrophils play a key role in the development of multiple organ failure after trauma. To have a detailed understanding of the neutrophil activation at primary stages after trauma, neutrophils were isolated from control and surgical trauma rats in this study. Extracted proteins were analyzed using nano liquid chromatography coupled to tandem mass spectrometry. A total of 2924 rat neutrophil proteins were identified in our analysis, of which 393 were found differentially regulated between control and trauma groups...
September 27, 2016: Proteomics. Clinical Applications
Zhuo Gan, Liya Ding, Christoph J Burckhardt, Jason Lowery, Assaf Zaritsky, Karlyndsay Sitterley, Andressa Mota, Nancy Costigliola, Colby G Starker, Daniel F Voytas, Jessica Tytell, Robert D Goldman, Gaudenz Danuser
Increased expression of vimentin intermediate filaments (VIFs) enhances directed cell migration, but the mechanism behind VIFs' effect on motility is not understood. VIFs interact with microtubules, whose organization contributes to polarity maintenance in migrating cells. Here, we characterize the dynamic coordination of VIF and microtubule networks in wounded monolayers of retinal pigment epithelial cells. By genome editing, we fluorescently labeled endogenous vimentin and α-tubulin, and we developed computational image analysis to delineate architecture and interactions of the two networks...
September 28, 2016: Cell Systems
Christopher D Hartman, Brett C Isenberg, Samantha G Chua, Joyce Y Wong
Mechanical compliance has been demonstrated to be a key determinant of cell behavior, directing processes such as spreading, migration, and differentiation. Durotaxis, directional migration from softer to more stiff regions of a substrate, has been observed for a variety of cell types. Recent stiffness mapping experiments have shown that local changes in tissue stiffness in disease are often accompanied by an altered ECM composition in vivo. However, the importance of ECM composition in durotaxis has not yet been explored...
October 4, 2016: Proceedings of the National Academy of Sciences of the United States of America
Cortney L Armstrong, Irina Miralda, Adam C Neff, Shifu Tian, Aruna Vashishta, Lisandra Perez, Junyi Le, Richard J Lamont, Silvia M Uriarte
Filifactor alocis, is a recently recognized periodontal pathogen, however little is known regarding its interactions with the immune system. As the first responder phagocytic cells, neutrophils are recruited in large numbers to the periodontal pocket, where they play a crucial role in the innate defense of the periodontium. Thus, in order to colonize, successful periodontal pathogens must devise means to interfere with neutrophil chemotaxis and activation. In this study, we assessed major neutrophil functions including degranulation and cell migration associated with the p38 MAPK signaling pathway upon challenge with F...
September 19, 2016: Infection and Immunity
Fabien Binamé, Aurélien Bidaud-Meynard, Laure Magnan, Léo Piquet, Bertille Montibus, Anne Chabadel, Frédéric Saltel, Valérie Lagrée, Violaine Moreau
Spatiotemporal regulation of RhoGTPases such as RhoA is required at the cell leading edge to achieve cell migration. p190RhoGAP (p190A) is the main negative regulator of RhoA and localizes to membrane protrusions, where its GTPase-activating protein (GAP) activity is required for directional migration. In this study, we investigated the molecular processes responsible for p190A targeting to actin protrusions. By analyzing the subcellular localization of truncated versions of p190A in hepatocellular carcinoma cells, we identified a novel functional p190A domain: the protrusion localization sequence (PLS) necessary and sufficient for p190A targeting to leading edges...
September 26, 2016: Journal of Cell Biology
Juliana Perrone Bezerra de Menezes, Amrita Koushik, Satarupa Das, Can Guven, Ariel Siegel, Maria Fernanda Laranjeira-Silva, Wolfgang Losert, Norma W Andrews
Leishmania is an intracellular protozoan parasite that causes a broad spectrum of clinical manifestations, ranging from self-healing skin lesions to fatal visceralizing disease. As the host cells of choice for all species of Leishmania, macrophages are critical for the establishment of infections. How macrophages contribute to parasite homing to specific tissues and how parasites modulate macrophage function are still poorly understood. In this study, we show that Leishmania amazonensis infection inhibits macrophage roaming motility...
September 18, 2016: Cellular Microbiology
J van de Kamp, V Paefgen, M Wöltje, M Böbel, J Jaekel, B Rath, N Labude, R Knüchel, W Jahnen-Dechent, Sabine Neuss
Mesenchymal stem cells (MSC) are precursor cells of mesodermal tissue and, because of their trophic phenotype, they are known to play beneficial roles in wound healing. In addition, various tissue engineering strategies are based on MSC/biomaterial constructs. As the isolation and expansion of MSCs is a long-term process, a major goal is to develop an endogenous stem cell recruitment system that circumvents all ex vivo steps generally used for tissue engineering. Therefore collagen and silk fibroin were loaded with hepatocyte growth factor (HGF), a chemoattractant for MSCs...
September 19, 2016: Journal of Tissue Engineering and Regenerative Medicine
Yue Wu, Michael Hannigan, Lijun Zhan, Joseph A Madri, Chi-Kuang Huang
Neutrophils from NOD (Non-Obese Diabetic) mice exhibited reduced migration speed, decreased frequency of directional changes and loss of directionality during chemotaxis (compared to wild type (WT) C57BL/6 mice). Additionally, F-actin of chemotaxing NOD neutrophils failed to orient toward the chemoattractant gradient and NOD neutrophil adhesion was impaired. A point mutation near the autophosphorylation site of Lyn in NOD mice was identified. Point mutations of G to A (G1412 in LynA and G1199 in LynB) cause a change of amino acid E393 (glutamic acid) to K (lysine) in LynA (E393 →K) (E372 of LynB), affecting fMLP-induced tyrosine phosphorylation...
September 3, 2016: Journal of Cellular Physiology
Alexander Braun, Nicole M Caesar, Kyvan Dang, Kenneth A Myers
The physiological process by which new vasculature forms from existing vasculature requires specific signaling events that trigger morphological changes within individual endothelial cells (ECs). These processes are critical for homeostatic maintenance such as wound healing, and are also crucial in promoting tumor growth and metastasis. EC morphology is defined by the organization of the cytoskeleton, a tightly regulated system of actin and microtubule (MT) dynamics that is known to control EC branching, polarity and directional migration, essential components of angiogenesis...
2016: Journal of Visualized Experiments: JoVE
Vincent Stepanik, Leslie Dunipace, Young-Kyung Bae, Frank Macabenta, Jingjing Sun, Nathanie Trisnadi, Angelike Stathopoulos
Caudal visceral mesoderm (CVM) cells migrate from posterior to anterior of the Drosophila embryo as two bilateral streams of cells to support the specification of longitudinal muscles along the midgut. To accomplish this long-distance migration, CVM cells receive input from their environment, but little is known about how this collective cell migration is regulated. In a screen we found that wunen mutants exhibit CVM cell migration defects. Wunens are lipid phosphate phosphatases known to regulate the directional migration of primordial germ cells (PGCs)...
September 1, 2016: Development
Gang Wang, Luyang Cao, Xiaowen Liu, Nathan A Sieracki, Anke Di, Xi Wen, Yong Chen, Shalina Taylor, Xiaojia Huang, Chinnaswamy Tiruppathi, You-Yang Zhao, Yuanlin Song, Xiaopei Gao, Tian Jin, Chunxue Bai, Asrar B Malik, Jingsong Xu
Blood neutrophils perform an essential host-defense function by directly migrating to bacterial invasion sites to kill bacteria. The mechanisms mediating the transition from the migratory to bactericidal phenotype remain elusive. Here, we demonstrate that TRPM2, a trp superfamily member, senses neutrophil-generated reactive oxygen species and restrains neutrophil migration. The inhibitory function of oxidant sensing by TRPM2 requires the oxidation of Cys549, which then induces TRMP2 binding to formyl peptide receptor 1 (FPR1) and subsequent FPR1 internalization and signaling inhibition...
September 12, 2016: Developmental Cell
Vitali Alexeev, Adele Donahue, Jouni Uitto, Olga Igoucheva
BACKGROUND: Dystrophic epidermolysis bullosa (DEB), a rare genodermatosis, is characterized by the formation of intra-epidermal blistering and the development of chronic nonhealing skin wounds. Recently, attempts have been made to develop cell-based therapies for this currently intractable disorder. The molecular mechanisms that govern directional migration of the adult stem cells, allowing their efficient and controlled homing to the skin affected with DEB, are poorly understood. The key mechanism that regulates recruitment of leukocytes and progenitor stem cells to distal anatomical tissues affected with disease is chemotaxis, which depends on the signaling molecules, chemokines, and acts primarily as part of the host defense and repair mechanism...
2016: Stem Cell Research & Therapy
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"