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"Directional migration"

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https://www.readbyqxmd.com/read/28078410/effects-of-direct-current-electric-fields-on-lung-cancer-cell-electrotaxis-in-a-pmma-based-microfluidic-device
#1
Yaping Li, Tao Xu, Xiaomei Chen, Shin Lin, Michael Cho, Dong Sun, Mengsu Yang
Tumor metastasis is the primary cause of cancer death. Numerous studies have demonstrated the electrotactic responses of various cancer cell types, and suggested its potential implications in metastasis. In this study, we used a microfluidic device to emulate endogenous direct current electric field (dcEF) environment, and studied the electrotactic migration of non-small cell lung cancer cell lines (H460, HCC827, H1299, and H1975) and the underlying mechanisms. These cell lines exhibited greatly different response in applied dcEFs (2-6 V/cm)...
January 11, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28078186/soluble-receptor-for-advanced-glycation-end-products-improves-stromal-cell-derived-factor-1-activity-in-model-diabetic-environments
#2
Melissa Przyborowski Olekson, Renea A Faulknor, Henry C Hsia, Ann Marie Schmidt, François Berthiaume
Objective: In diabetes, hyperglycemia causes the accumulation of advanced glycation end products (AGEs) that trigger reactive oxygen species (ROS) generation through binding the receptor for AGEs (RAGE). Because exogenous growth factors have had little success in enhancing chronic wound healing, we investigated whether hyperglycemia-induced AGEs interfere with cellular responses to extracellular signals. We used stromal cell-derived factor-1 (SDF-1), an angiogenic chemokine also known to promote stem cell recruitment in skin wounds...
December 1, 2016: Advances in Wound Care
https://www.readbyqxmd.com/read/28063647/collective-dynamics-of-cancer-cells-confined-in-a-confluent-monolayer-of-normal-cells
#3
Shao-Zhen Lin, Bo Li, Guang-Kui Xu, Xi-Qiao Feng
Tumorigenesis often involves specific changes in cell motility and intercellular adhesion. Understanding the collective cancer cell behavior associated with these specific changes could facilitate the detection of malignant characteristics during tumor growth and invasion. In this study, a cellular vertex model is developed to investigate the collective dynamics of a disk-like aggregate of cancer cells confined in a confluent monolayer of normal cells. The effects of intercellular adhesion and cell motility on tumor progression are examined...
December 29, 2016: Journal of Biomechanics
https://www.readbyqxmd.com/read/28051852/artificial-slanted-nanocilia-array-as-a-mechanotransducer-for-controlling-cell-polarity
#4
Hong Nam Kim, Kyung-Jin Jang, Jung-Youn Shin, Daeshik Kang, Sang Moon Kim, Ilkyoo Koh, Yoonmi Hong, Segeun Jang, Min Sung Kim, Byung-Soo Kim, Hoon Eui Jeong, Noo Li Jeon, Pilnam Kim, Kahp-Yang Suh
We present a method to induce cell directional behavior using slanted nanocilia arrays. NIH-3T3 fibroblasts demonstrated bidirectional polarization in a rectangular arrangement on vertical nanocilia arrays and exhibited a transition from a bidirectional to a unidirectional polarization pattern when the angle of the nanocilia was decreased from 90° to 30°. The slanted nanocilia guided and facilitated spreading by allowing the cells to contact the sidewalls of the nanocilia, and the directional migration of the cells opposed the direction of the slant due to the anisotropic bending stiffness of the slanted nanocilia...
January 4, 2017: ACS Nano
https://www.readbyqxmd.com/read/28039755/myosin-phosphorylation-on-stress-fibers-predicts-contact-guidance-behavior-across-diverse-breast-cancer-cells
#5
Juan Wang, Ian C Schneider
During cancer progression the extracellular matrix is remodeled, forming aligned collagen fibers that proceed radially from the tumor, resulting in invasion. We have recently shown that different invasive breast cancer cells respond to epitaxially grown, aligned collagen fibrils differently. This article develops insight into why these cells differ in their contact guidance fidelity. Small changes in contractility or adhesion dramatically alter directional persistence on aligned collagen fibrils, while migration speed remains constant...
November 28, 2016: Biomaterials
https://www.readbyqxmd.com/read/28017473/huntingtin-mediated-multipolar-bipolar-transition-of-newborn-cortical-neurons-is-critical-for-their-postnatal-neuronal-morphology
#6
Monia Barnat, Julien Le Friec, Caroline Benstaali, Sandrine Humbert
In the developing cortex, projection neurons undergo multipolar-bipolar transition, radial-directed migration, and maturation. The contribution of these developmental steps to the structure of the adult cortex is not completely understood. Here, we report that huntingtin (HTT), the protein mutated in Huntington's disease, is enriched in polarizing projection neurons. The depletion of HTT in postmitotic projection neurons leads to the mislocalization of layer-specific neuronal populations in the mouse neocortex...
January 4, 2017: Neuron
https://www.readbyqxmd.com/read/28008721/therapy-of-primary-and-metastatic-liver-cancer-by-human-ips-cell-derived-myeloid-cells-producing-interferon-%C3%AE
#7
Masataka Sakisaka, Miwa Haruta, Yoshihiro Komohara, Satoshi Umemoto, Keiko Matsumura, Tokunori Ikeda, Motohiro Takeya, Yukihiro Inomata, Yasuharu Nishimura, Satoru Senju
BACKGROUND: iPS-ML are myeloid lineage cells with a proliferative capacity derived from induced pluripotent stem (iPS) cells. This study aimed to examine therapeutic effect of iPS-ML producing interferon-β (iPS-ML/IFN-β) towards primary and metastatic liver cancer and investigate the mechanism of that effect. METHODS: We established a xenograft model of liver metastasis by injecting the spleen of SCID mice with MKN-45 human gastric cancer cells and also a primary liver cancer model by injecting SK-HEP-1 human hepatocellular carcinoma cells into the liver...
December 23, 2016: Journal of Hepato-biliary-pancreatic Sciences
https://www.readbyqxmd.com/read/27998813/controlling-the-selective-and-directional-migration-of-hepatocytes-by-a-complementary-density-gradient-of-glycosylated-hyperbranched-polymers-and-poly-ethylene-glycol-molecules
#8
Su Liang, Shan Yu, Ning Zhou, Jun Deng, Changyou Gao
: Repair and regeneration of defected tissues and organs depends strongly on the directional migration of targeted cells, for example, the enhancement of directional migration of hepatocytes could be helpful in liver regeneration and transplantation. Herein a complementary gradient of galactose-modified hyperbranched polymers (LA-HPMA) and poly(ethylene glycol) (PEG) molecules was designed and prepared on a same substrate. Characterizations of X-ray photoelectron spectrometry and quartz crystal microbalance with dissipation (QCM-d) demonstrated the unidirectional change in grafting density of LA-HPMA and PEG molecules, respectively...
December 17, 2016: Acta Biomaterialia
https://www.readbyqxmd.com/read/27997826/repulsive-epithelial-cues-direct-glial-migration-along-the-nerve
#9
Sofia Sasse, Christian Klämbt
Most glial cells show pronounced migratory abilities and generally follow axonal trajectories to reach their final destination. However, the molecular cues controlling their directional migration are largely unknown. To address this, we established glial migration onto the developing Drosophila leg imaginal disc as a model. Here, CNS-derived glial cells move along nerves containing motoaxons and sensory axons. Along their path, glial cells encounter at least three choice points where directional decisions are needed...
December 19, 2016: Developmental Cell
https://www.readbyqxmd.com/read/27974574/sphingosine-1-phosphate-receptor-1-regulates-the-directional-migration-of-lymphatic-endothelial-cells-in-response-to-fluid-shear-stress
#10
Vinay N Surya, Eleftheria Michalaki, Eva Y Huang, Gerald G Fuller, Alexander R Dunn
The endothelial cells that line blood and lymphatic vessels undergo complex, collective migration and rearrangement processes during embryonic development, and are known to be exquisitely responsive to fluid flow. At present, the molecular mechanisms by which endothelial cells sense fluid flow remain incompletely understood. Here, we report that both the G-protein-coupled receptor sphingosine 1-phosphate receptor 1 (S1PR1) and its ligand sphingosine 1-phosphate (S1P) are required for collective upstream migration of human lymphatic microvascular endothelial cells in an in vitro setting...
December 2016: Journal of the Royal Society, Interface
https://www.readbyqxmd.com/read/27968877/health-status-and-migration-a-propensity-score-matching-with-difference-in-difference-regression-approach
#11
Liming Lu, Xiao Gong, Jingchun Zeng
BACKGROUND: By 2014, about 253 million Chinese migrants had left their rural hometowns and were working in cities. The effect of migration on health is unclear, since it is affected by changes in environments and behaviours over the course of migration. Furthermore, previous studies have not thoroughly considered the "healthy migrant hypothesis" (that is, healthy people are more conducive to migrate). This paper strives to explore the potential mechanisms of migration and health over two successive years to provide a useful reference point for health policy targeted at migrants...
October 2016: Lancet
https://www.readbyqxmd.com/read/27965956/diacylglycerol-kinases-shaping-diacylglycerol-and-phosphatidic-acid-gradients-to-control-cell-polarity
#12
REVIEW
Gianluca Baldanzi, Valentina Bettio, Valeria Malacarne, Andrea Graziani
Diacylglycerol kinases (DGKs) terminate diacylglycerol (DAG) signaling and promote phosphatidic acid (PA) production. Isoform specific regulation of DGKs activity and localization allows DGKs to shape the DAG and PA gradients. The capacity of DGKs to constrain the areas of DAG signaling is exemplified by their role in defining the contact interface between T cells and antigen presenting cells: the immune synapse. Upon T cell receptor engagement, both DGK α and ζ metabolize DAG at the immune synapse thus constraining DAG signaling...
2016: Frontiers in Cell and Developmental Biology
https://www.readbyqxmd.com/read/27965424/chemoattractant-concentration-dependent-tuning-of-erk-signaling-dynamics-in-migrating-neutrophils
#13
Elizabeth R Zhang, Shanshan Liu, Lani F Wu, Steven J Altschuler, Melanie H Cobb
The directed migration (chemotaxis) of neutrophils toward the bacterial peptide N-formyl-Met-Leu-Phe (fMLP) is a crucial process in immune defense against invading bacteria. While navigating through a gradient of increasing concentrations of fMLP, neutrophils and neutrophil-like HL-60 cells switch from exhibiting directional migration at low fMLP concentrations to exhibiting circuitous migration at high fMLP concentrations. The extracellular signal-regulated kinase (ERK) pathway is implicated in balancing this fMLP concentration-dependent switch in migration modes...
December 13, 2016: Science Signaling
https://www.readbyqxmd.com/read/27938938/overexpression-of-hif-1%C3%AE-in-mesenchymal-stem-cells-contributes-to-repairing-hypoxic-ischemic-brain-damage-in-rats
#14
Deju Lin, Liping Zhou, Biao Wang, Lizhen Liu, Li Cong, Chuanqin Hu, Tingting Ge, Qin Yu
Preclinical researches on mesenchymal stem cells (MSCs) transplantation, which is used to treat hypoxic-ischemic (HI) brain damage, have received inspiring achievements. However, the insufficient migration of active cells to damaged tissues has limited their potential therapeutic effects. There are some evidences that hypoxia inducible factor-1 alpha (HIF-1α) promotes the viability and migration of the cells. Here, we aim to investigate whether overexpression of HIF-1α in MSCs could improve the viability and migration capacity of cells, and its therapeutic efficiency on HI brain damage...
December 6, 2016: Comptes Rendus Biologies
https://www.readbyqxmd.com/read/27922761/%C3%AE-6%C3%AE-4-integrin-regulates-the-collective-migration-of-epithelial-cells
#15
Zachary T Colburn, Jonathan C R Jones
α6β4 integrin is localized in a unique punctate distribution at the cell-substratum interface along the leading front of single, front-rear polarized, A549 cells. These puncta are interspersed between focal adhesions and lack association with the actin cytoskeleton. Knockdown of β4 integrin in A549 cells inhibits their directed migration, with knockdown cells exhibiting large focal adhesions and reduced actin dynamics. Despite these changes, the speed of knockdown cells is equivalent to controls cells. Interestingly, in such cells, α6 integrin retains its punctate distribution...
December 6, 2016: American Journal of Respiratory Cell and Molecular Biology
https://www.readbyqxmd.com/read/27916905/drug-release-by-direct-jump-from-poly-ethylene-glycol-b-%C3%AE%C2%B5-caprolactone-nano-vector-to-cell-membrane
#16
Ugo Till, Laure Gibot, Anne-Françoise Mingotaud, Jérôme Ehrhart, Luc Wasungu, Christophe Mingotaud, Jean-Pierre Souchard, Alix Poinso, Marie-Pierre Rols, Frédéric Violleau, Patricia Vicendo
Drug delivery by nanovectors involves numerous processes, one of the most important being its release from the carrier. This point still remains unclear. The current work focuses on this point using poly(ethyleneglycol-b-ε-caprolactone) micelles containing either pheophorbide-a (Pheo-a) as a fluorescent probe and a phototoxic agent or fluorescent copolymers. This study showed that the cellular uptake and the phototoxicity of loaded Pheo-a are ten times higher than those of the free drug and revealed a very low cellular penetration of the fluorescence-labeled micelles...
November 30, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/27916465/hypoxia-inhibits-lymphatic-thoracic-duct-formation-in-zebrafish
#17
Isabelle Ernens, Andrew I Lumley, Lu Zhang, Yvan Devaux, Daniel R Wagner
Hypoxia promotes blood vessel growth through up-regulation of pro-angiogenic pathways but its role on the lymphatic system remains unclear. The homeobox transcription factor Prox1 is a master control gene for generating lymphatic endothelial cells (LECs) and is up-regulated by hypoxia-inducible factors in mammals. While vascular endothelial growth factor A (VEGFA) is critical for angiogenesis, VEGFC and its receptor VEGF receptor-3 (VEGFR-3) are essential for the initial sprouting and directed migration as well as for the subsequent survival of LECs...
January 22, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/27913681/the-borg-family-of-cdc42-effector-proteins-cdc42ep1-5
#18
REVIEW
Aaron J Farrugia, Fernando Calvo
Despite being discovered more than 15 years ago, the Borg (binder of Rho GTPases) family of Cdc42 effector proteins (Cdc42EP1-5) remains largely uncharacterised and relatively little is known about their structure, regulation and role in development and disease. Recent studies are starting to unravel some of the key functional and mechanistic aspects of the Borg proteins, including their role in cytoskeletal remodelling and signalling. In addition, the participation of Borg proteins in important cellular processes such as cell shape, directed migration and differentiation is slowly emerging, directly linking Borgs with important physiological and pathological processes such as angiogenesis, neurotransmission and cancer-associated desmoplasia...
December 15, 2016: Biochemical Society Transactions
https://www.readbyqxmd.com/read/27904998/alginate-chitosan-hydrogels-provide-a-sustained-gradient-of-sphingosine-1-phosphate-for-therapeutic-angiogenesis
#19
Priscilla A Williams, Kevin T Campbell, Hessam Gharaviram, Justin L Madrigal, Eduardo A Silva
Sphingosine-1-phosphate (S1P), a bioactive lipid, is a potent candidate for treatment of ischemic vascular disease. However, designing biomaterial systems for the controlled release of S1P to achieve therapeutic angiogenesis presents both biological and engineering challenges. Thus, the objective of this study was to design a hydrogel system that provides controlled and sustained release of S1P to establish local concentration gradients that promote neovascularization. Alginate hydrogels have been extensively studied and characterized for delivery of proangiogenic factors...
November 30, 2016: Annals of Biomedical Engineering
https://www.readbyqxmd.com/read/27902969/rac1-gtp-ase-signals-wnt-beta-catenin-pathway-mediated-integrin-directed-metastasis-associated-tumor-cell-phenotypes-in-triple-negative-breast-cancers
#20
Pradip De, Jennifer H Carlson, Tyler Jepperson, Scooter Willis, Brian Leyland-Jones, Nandini Dey
The acquisition of integrin-directed metastasis-associated (ID-MA) phenotypes by Triple-Negative Breast Cancer (TNBC) cells is caused by an upregulation of the Wnt-beta-catenin pathway (WP). We reported that WP is one of the salient genetic features of TNBC. RAC-GTPases, small G-proteins which transduce signals from cell surface proteins including integrins, have been implicated in tumorigenesis and metastasis by their role in essential cellular functions like motility. The collective percentage of alteration(s) in RAC1 in ER+ve BC was lower as compared to ER-ve BC (35% vs 57%) (brca/tcga/pub2015)...
November 25, 2016: Oncotarget
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