keyword
MENU ▼
Read by QxMD icon Read
search

"Directional migration"

keyword
https://www.readbyqxmd.com/read/28817611/the-directed-migration-of-gonadal-distal-tip-cells-in-caenorhabditis-elegans-requires-ngat-1-a-%C3%A3-1-4-n-acetylgalactosaminyltransferase-enzyme
#1
Joseph Veyhl, Robert J Dunn, Wendy L Johnston, Alexa Bennett, Lijia W Zhang, James W Dennis, Harry Schachter, Joseph G Culotti
Glycoproteins such as growth factor receptors and extracellular matrix have well-known functions in development and cancer progression, however, the glycans at sites of modification are often heterogeneous molecular populations which makes their functional characterization challenging. Here we provide evidence for a specific, discrete, well-defined glycan modification and regulation of a stage-specific cell migration in Caenorhabditis elegans. We show that a chain-terminating, putative null mutation in the gene encoding a predicted β1,4-N-acetylgalactosaminyltransferase, named ngat-1, causes a maternally rescued temperature sensitive (ts) defect in the second phase of the three phase migration pattern of the posterior, but not the anterior, hermaphrodite Distal Tip Cell (DTC)...
2017: PloS One
https://www.readbyqxmd.com/read/28805878/interfacial-entropy-driven-thermophoretic-tweezers
#2
Linhan Lin, Xiaolei Peng, Zhangming Mao, Xiaoling Wei, Chong Xie, Yuebing Zheng
Directed migration of particles and molecules in a temperature gradient field, which is known as thermophoresis or the Soret effect, is of fundamental importance for mass transfer in colloid science and life sciences. However, thermophoretic tweezers that enable versatile particle manipulation have remained elusive due to the complex underlying physical forces in thermophoresis and the lack of general thermophilic particles above room temperature. Herein, we exploit entropic response and permittivity gradient at the particle-solvent interface to optically generated thermal gradient to achieve the thermophoretic trapping and dynamic manipulation of charged particles over an optothermal-responsive substrate...
August 14, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28793794/excitable-signal-transduction-networks-in-directed-cell-migration
#3
Peter N Devreotes, Sayak Bhattacharya, Marc Edwards, Pablo A Iglesias, Thomas Lampert, Yuchuan Miao
Although directed migration of eukaryotic cells may have evolved to escape nutrient depletion, it has been adopted for an extensive range of physiological events during development and in the adult organism. The subversion of these movements results in disease, such as cancer. Mechanisms of propulsion and sensing are extremely diverse, but most eukaryotic cells move by extending actin-filled protrusions termed macropinosomes, pseudopodia, or lamellipodia or by extension of blebs. In addition to motility, directed migration involves polarity and directional sensing...
August 9, 2017: Annual Review of Cell and Developmental Biology
https://www.readbyqxmd.com/read/28771176/regulation-of-chemokine-function-the-roles-of-gag-binding-and-post-translational-nitration
#4
REVIEW
Sarah Thompson, Beatriz Martínez-Burgo, Krishna Mohan Sepuru, Krishna Rajarathnam, John A Kirby, Neil S Sheerin, Simi Ali
The primary function of chemokines is to direct the migration of leukocytes to the site of injury during inflammation. The effects of chemokines are modulated by several means, including binding to G-protein coupled receptors (GPCRs), binding to glycosaminoglycans (GAGs), and through post-translational modifications (PTMs). GAGs, present on cell surfaces, bind chemokines released in response to injury. Chemokines bind leukocytes via their GPCRs, which directs migration and contributes to local inflammation...
August 3, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28767395/prohibitin-relocated-to-the-front-ends-can-control-the-migration-directionality-of-colorectal-cancer-cells
#5
Li-Li Ma, Lan Shen, Gui-Hui Tong, Na Tang, Yang Luo, Li-Li Guo, Chun-Ting Hu, Ying-Xin Huang, Guan Huang, Fang-Yan Jing, Chao Liu, Zhuo-Yi Li, Na Zhou, Qian-Wen Yan, Yan Lei, Shi-Jie Zhu, Zhi-Qiang Cheng, Guang-Wen Cao, Yong-Jian Deng, Yan-Qing Ding
Directional migration is a cost-effective movement allowing invasion and metastatic spread of cancer cells. Although migration related to cytoskeletal assembly and microenvironmental chemotaxis has been elucidated, little is known about interaction between extracellular and intracellular molecules for controlling the migrational directionality. A polarized expression of prohibitin (PHB) in the front ends of CRC cells favors metastasis and is correlated with poor prognosis for 545 CRC patients. A high level of vascular endothelial growth factor (VEGF) in the interstitial tissue of CRC patients is associated with metastasis...
July 19, 2017: Oncotarget
https://www.readbyqxmd.com/read/28754687/cells-lay-their-own-tracks-optogenetic-cdc42-activation-stimulates-fibronectin-deposition-supporting-directed-migration
#6
Seth P Zimmerman, Sreeja B Asokan, Brian Kuhlman, James E Bear
Rho GTPase family members are known regulators of directed migration and therefore play key roles in processes including development, immune response and cancer metastasis. However, their individual contributions to these processes are complex. Here, we regulate the activity of two family members, Rac and Cdc42, by optogenetically recruiting specific GEF DH/PH domains to defined regions on the cell membrane. We find that the localized activation of both GTPases produce lamellipodia in cells plated on a fibronectin substrate...
July 28, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28754685/engineering-mammalian-cells-to-seek-senescence-associated-secretory-phenotypes
#7
Anam Qudrat, Janice Wong, Kevin Truong
Since the removal of senescent cells in model organisms has been linked to rejuvenation and increased lifespan, senotherapies have emerged to target senescent cells for death. In particular, interleukin-6 (IL6) is a prominent senescence associated secretory phenotype (SASP) and thus, seeking IL6 could potentially localize engineered cells to senescent cells for therapeutic intervention. Here, we engineered a chimeric IL6 receptor (IL6Rchi) that generates a Ca(2+) signal in response to IL6 stimulation. When IL6Rchi was co-expressed with an engineered Ca(2+)-activated RhoA (CaRQ), it enabled directed migration to IL6 in cells that have no such natural ability...
July 28, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28730381/pooled-human-serum-increases-regenerative-potential-of-in-vitro-expanded-stem-cells-from-human-extracted-deciduous-teeth
#8
Nazmul Haque, Noor Hayaty Abu Kasim
In regenerative therapy, in vitro expansion of stem cells is critical to obtain a significantly higher number of cells for successful engraftment after transplantation. However, stem cells lose its regenerative potential and enter senescence during in vitro expansion. In this study, the influence of foetal bovine serum (FBS) and pooled human serum (pHS) on the proliferation, morphology and migration of stem cells from human extracted deciduous teeth (SHED) was compared. SHED (n = 3) was expanded in KnockOut DMEM supplemented with either pHS (pHS-SM) or FBS (FBS-SM)...
July 21, 2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/28725484/logarithmic-sensing-in-bacillus-subtilis-aerotaxis
#9
Filippo Menolascina, Roberto Rusconi, Vicente I Fernandez, Steven Smriga, Zahra Aminzare, Eduardo D Sontag, Roman Stocker
Aerotaxis, the directed migration along oxygen gradients, allows many microorganisms to locate favorable oxygen concentrations. Despite oxygen's fundamental role for life, even key aspects of aerotaxis remain poorly understood. In Bacillus subtilis, for example, there is conflicting evidence of whether migration occurs to the maximal oxygen concentration available or to an optimal intermediate one, and how aerotaxis can be maintained over a broad range of conditions. Using precisely controlled oxygen gradients in a microfluidic device, spanning the full spectrum of conditions from quasi-anoxic to oxic (60 n mol/l-1 m mol/l), we resolved B...
2017: NPJ Systems Biology and Applications
https://www.readbyqxmd.com/read/28716951/a-calcium-diacylglycerol-stimulated-protein-kinase-c-c-elegans-pkc-2-links-thermal-signals-to-learned-behavior-by-acting-in-a-sensory-neuron-and-intestinal-cells
#10
Marianne Land, Charles S Rubin
Ca(2+) and diacylglycerol (DAG) activated protein kinase C (cPKC) promotes learning and behavioral plasticity. However, knowledge of in vivo regulation and exact functions of cPKCs that affect behavior is limited. We show that PKC-2, a C. elegans cPKC, is essential for a complex behavior, thermotaxis. C. elegans memorizes a nutrient-associated cultivation temperature (Tc) and migrates along Tc within a 17-25°C gradient. pkc-2 gene disruption abrogated thermotaxis; a PKC-2 transgene, driven by endogenous pkc-2 promoters, restored thermotaxis behavior in pkc-2(-/-) animals...
July 17, 2017: Molecular and Cellular Biology
https://www.readbyqxmd.com/read/28714585/mathematical-model-of-collective-cell-migrations-based-on-cell-polarity
#11
Masakazu Akiyama, Takamichi Sushida, Sumire Ishida, Hisashi Haga
Individual cells migrate toward the direction of the cell polarity generated by interior or exterior factors. Under situations without guides such as chemoattractants, they migrate randomly. On the other hand, it has been observed that cell groups lead to systematic collective cell migrations. For example, Dictyostelium discoideum and Madin-Darby canine kidney (epithelial) cells exhibit typical collective cell migration patterns such as uniformly directional migration and rotational migration. In particular, it has been suggested from experimental investigations that rotational migrations are intimately related to morphogenesis of organs and tissues in several species...
July 17, 2017: Development, Growth & Differentiation
https://www.readbyqxmd.com/read/28700916/a-reaction-diffusion-model-explains-amplification-of-the-plc-pkc-pathway-in-fibroblast-chemotaxis
#12
Krithika Mohan, Jamie L Nosbisch, Timothy C Elston, James E Bear, Jason M Haugh
During the proliferative phase of cutaneous wound healing, dermal fibroblasts are recruited into the clotted wound by a concentration gradient of platelet-derived growth factor (PDGF), together with other spatial cues. Despite the importance of this chemotactic process, the mechanisms controlling the directed migration of slow-moving mesenchymal cells such as fibroblasts are not well understood. Here, we develop and analyze a reaction-diffusion model of phospholipase C/protein kinase C (PKC) signaling, which was recently identified as a requisite PDGF-gradient-sensing pathway, with the goal of identifying mechanisms that can amplify its sensitivity in the shallow external gradients typical of chemotaxis experiments...
July 11, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/28687774/andean-mountain-building-did-not-preclude-dispersal-of-lowland-epiphytic-orchids-in-the-neotropics
#13
Oscar Alejandro Pérez-Escobar, Marc Gottschling, Guillaume Chomicki, Fabien L Condamine, Bente B Klitgård, Emerson Pansarin, Günter Gerlach
The Andean uplift is one of the major orographic events in the New World and has impacted considerably the diversification of numerous Neotropical lineages. Despite its importance for biogeography, the specific role of mountain ranges as a dispersal barrier between South and Central American lowland plant lineages is still poorly understood. The swan orchids (Cycnoches) comprise ca 34 epiphytic species distributed in lowland and pre-montane forests of Central and South America. Here, we study the historical biogeography of Cycnoches to better understand the impact of the Andean uplift on the diversification of Neotropical lowland plant lineages...
July 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28687663/a-module-for-rac-temporal-signal-integration-revealed-with-optogenetics
#14
Brian R Graziano, Delquin Gong, Karen E Anderson, Anne Pipathsouk, Anna R Goldberg, Orion D Weiner
Sensory systems use adaptation to measure changes in signaling inputs rather than absolute levels of signaling inputs. Adaptation enables eukaryotic cells to directionally migrate over a large dynamic range of chemoattractant. Because of complex feedback interactions and redundancy, it has been difficult to define the portion or portions of eukaryotic chemotactic signaling networks that generate adaptation and identify the regulators of this process. In this study, we use a combination of optogenetic intracellular inputs, CRISPR-based knockouts, and pharmacological perturbations to probe the basis of neutrophil adaptation...
August 7, 2017: Journal of Cell Biology
https://www.readbyqxmd.com/read/28685442/directional-migration-of-mda-mb-231-cells-under-o2-ph-gradients
#15
Y Enokida, Y Tsuruno, K Okubo, Y Yamaoka, E Takahashi
We hypothesized that cancer cells actively migrate toward intratumor microvessels, guided by tissue gradients of metabolic substrates (such as O2) and/or metabolites (such as CO2/H(+)). To test this hypothesis, we developed an in vitro model in which cellular energy metabolism establishes gradients of O2/nutrient/metabolite in monolayer cells cultured in a conventional culture dish. When gradients of O2 ranging from 3% to ~0% were produced, MDA-MB-231 cells located at 300, 500 and 1500 μm downstream in the gradient demonstrated significant directional migrations (Rayleigh z test)...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/28680155/polarized-distribution-of-active-myosin-ii-regulates-directional-migration-of-cultured-olfactory-ensheathing-cells
#16
Cheng-Gen Zheng, Fan Zhang, Xiao-Mei Bao, Shi-Yang Wu, Peng Wang, Jia-Nan Zhou, Yuan Gao, Hong-Lin Teng, Ying Wang, Zhi-Hui Huang
Migration of olfactory ensheathing cells (OECs) is critical for development of olfactory system and essential for neural regeneration after OEC transplantation into nerve injury site. However, the molecular mechanisms underlying the regulation of directional migration of OECs remain unclear. In this study, we found that in migrating OECs, phosphorylated myosin light chain (p-MLC, active myosin II) displayed a polarized distribution, with the leading front exhibiting higher than soma and trailing process. Over-expression of GFP-MLC significantly reduced OEC migration...
July 5, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28668623/electrotaxis-of-cardiac-progenitor-cells-cardiac-fibroblasts-and-induced-pluripotent-stem-cell-derived-cardiac-progenitor-cells-requires-serum-and-is-directed-via-pi3-k-pathways
#17
Bert J Frederich, Valeriy Timofeyev, Phung N Thai, Michael J Haddad, Adam Poe, Victor C Lau, Maryam Moshref, Anne A Knowlton, Padmini Sirish, Nipavan Chiamvimonvat
BACKGROUND: The limited regenerative capacity of cardiac tissue has long been an obstacle to treating damaged myocardium. Cell-based therapy offers an enormous potential to the current treatment paradigms. However, the efficacy of regenerative therapies remains limited by inefficient delivery and engraftment. Electrotaxis, electrically guided cell movement, has been clinically utilized to improve recovery in a number of tissues, but has not been investigated for treating myocardial damage...
June 28, 2017: Heart Rhythm: the Official Journal of the Heart Rhythm Society
https://www.readbyqxmd.com/read/28665039/anterior-gradient-2-agr2-is-induced-in-cutaneous-wound-and-promotes-wound-healing-through-its-adhesion-domain
#18
Qi Zhu, Hitesh Bhagavanbhai Mangukiya, Dhahiri Saidi Mashausi, Hao Guo, Hema Negi, Siva Bharath Merugu, Zhenghua Wu, Dawei Li
Anterior Gradient 2 (AGR2), a member of protein disulfide isomerase (PDI) family, is both located in cytoplasm and secreted into extracellular matrix. The orthologs of AGR2 have been linked to limb regeneration in newt and wound healing in zebrafish. In mammals, AGR2 influences multiple cell signaling pathways in tumor formation as well as in normal cell functions related to new tissue formation like angiogenesis. However, the function of AGR2 in mammalian wound healing remains unknown. This study aimed to investigate AGR2 expression and its function during skin wound healing and the possible application of external AGR2 in cutaneous wound to accelerate the healing process...
June 30, 2017: FEBS Journal
https://www.readbyqxmd.com/read/28662216/increased-dermal-collagen-bundle-alignment-in-systemic-sclerosis-is-associated-with-a-cell-migration-signature-and-role-of-arhgdib-in-directed-fibroblast-migration-on-aligned-ecms
#19
Lizhi Cao, Robert Lafyatis, Linda C Burkly
Systemic sclerosis (SSc) is a devastating disease affecting the skin and internal organs. Dermal fibrosis manifests early and Modified Rodnan Skin Scores (MRSS) correlate with disease progression. Transcriptomics of SSc skin biopsies suggest the role of the in vivo microenvironment in maintaining the pathological myofibroblasts. Therefore, defining the structural changes in dermal collagen in SSc patients could inform our understanding of fibrosis pathogenesis. Here, we report a method for quantitative whole-slide image analysis of dermal collagen from SSc patients, and our findings of more aligned dermal collagen bundles in diffuse cutaneous SSc (dcSSc) patients...
2017: PloS One
https://www.readbyqxmd.com/read/28649338/estimating-and-interpreting-migration-of-amazonian-forests-using-spatially-implicit-and-semi-explicit-neutral-models
#20
Edwin Pos, Juan Ernesto Guevara Andino, Daniel Sabatier, Jean-François Molino, Nigel Pitman, Hugo Mogollón, David Neill, Carlos Cerón, Gonzalo Rivas-Torres, Anthony Di Fiore, Raquel Thomas, Milton Tirado, Kenneth R Young, Ophelia Wang, Rodrigo Sierra, Roosevelt García-Villacorta, Roderick Zagt, Walter Palacios Cuenca, Milton Aulestia, Hans Ter Steege
With many sophisticated methods available for estimating migration, ecologists face the difficult decision of choosing for their specific line of work. Here we test and compare several methods, performing sanity and robustness tests, applying to large-scale data and discussing the results and interpretation. Five methods were selected to compare for their ability to estimate migration from spatially implicit and semi-explicit simulations based on three large-scale field datasets from South America (Guyana, Suriname, French Guiana and Ecuador)...
June 2017: Ecology and Evolution
keyword
keyword
14048
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"