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Directional migration

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https://www.readbyqxmd.com/read/29236328/mirnalet-7a-mediates-prostate-cancer-pc-3-cell-invasion-migration-by-inducing-epithelial-mesenchymal-transition-through-ccr7-mapk-pathway
#1
Tang Guanlin, Du Ruoyang, Tang Zhaobing, Kuang Youlin
Prostate cancer is one of the most common malignancies in older men. Recent evidence has demonstrated microRNA (miRNA) Let-7a expression decreased in prostate cancer, while the expression of C-C chemokine receptor type 7 (CCR7) increased. In this study, we investigated whether CCR7 overexpression was associated with a decrease in the expression of miRNA let-7a in invasion and metastasis of prostate cancer cell. Methods Synthetic let-7a mimics and let-7a inhibitors were transfected into prostate cancer PC-3 cells repectively...
December 13, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29235973/linear-array-of-multi-substrate-tracts-for-simultaneous-assessment-of-cell-adhesion-migration-and-differentiation
#2
Ricardo A Moreno-Rodriguez, Edward L Krug, Leticia Reyes, Roger R Markwald
Cell migration, which is central to a wide variety of life processes, involves integration of the extracellular matrix (ECM) with the internal cytoskeleton and motor proteins via receptors spanning the plasma membrane. Cell migration can be induced by a variety of signals, including gradients of external soluble molecules, differences in ECM composition, or electrical gradients. Current in vitro methods to study cell migration only test one substrate at a time. Here, we present a method for assessing cell adhesion, migration, and differentiation in up to 20 different test conditions simultaneously, using only minute amounts of target substrate...
December 1, 2017: BioTechniques
https://www.readbyqxmd.com/read/29235514/substrate-stiffness-influences-phenotype-and-function-of-human-antigen-presenting-dendritic-cells
#3
Svenja F B Mennens, Matteo Bolomini-Vittori, Jorieke Weiden, Ben Joosten, Alessandra Cambi, Koen van den Dries
Dendritic cells (DCs) are specialized immune cells that scan peripheral tissues for foreign material or aberrant cells and, upon recognition of such danger signals, travel to lymph nodes to activate T cells and evoke an immune response. For this, DCs travel large distances through the body, encountering a variety of microenvironments with different mechanical properties such as tissue stiffness. While immune-related pathological conditions such as fibrosis or cancer are associated with tissue stiffening, the role of tissue stiffness in regulating key functions of DCs has not been studied yet...
December 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29234238/mir-155-5p-modulates-malignant-behaviors-of-hepatocellular-carcinoma-by-directly-targeting-cthrc1-and-indirectly-regulating-gsk-3%C3%AE-involved-wnt-%C3%AE-catenin-signaling
#4
Gang Chen, Dongdong Wang, Xiongqi Zhao, Jun Cao, Yingpeng Zhao, Fan Wang, Jianhua Bai, Ding Luo, Li Li
Background: Hepatocellular carcinoma (HCC) remains one of the most lethal cancers. MicroRNA-155 (miR-155) and collagen triple helix repeat containing 1 (CTHRC1) were found to be involved in hepatocarcinogenesis, but their detailed functions in HCC are unclear. Here, we aimed to investigate the underlying role of miR-155-5p and CTHRC1 in HCC. Methods: miR-155-5p and CTHRC1 expression levels were detected by qRT-PCR, IHC and WB in HCC patients and cell lines. Dual-luciferase assay, qRT-PCR and WB were used to validate the target interaction between miR-155-5p and CTHRC1...
2017: Cancer Cell International
https://www.readbyqxmd.com/read/29234151/srgap1-a-crucial-target-of-mir-340-and-mir-124-functions-as-a-potential-oncogene-in-gastric-tumorigenesis
#5
Tingting Huang, Yuhang Zhou, Jinglin Zhang, Chi Chun Wong, Weilin Li, Johnny S H Kwan, Rui Yang, Aden K Y Chan, Yujuan Dong, Feng Wu, Bin Zhang, Alvin H K Cheung, William K K Wu, Alfred S L Cheng, Jun Yu, Nathalie Wong, Wei Kang, Ka Fai To
Slit-Robo GTPase-activating protein 1 (SRGAP1) functions as a GAP for Rho-family GTPases and downstream of Slit-Robo signaling. We aim to investigate the biological function of SRGAP1 and reveal its regulation by deregulated microRNAs (miRNAs) in gastric cancer (GC). mRNA and protein expression of SRGAP1 were examined by quantitative reverse transcription PCR (qRT-PCR) and western blot. The biological role of SRGAP1 was demonstrated through siRNA-mediated knockdown experiments. The regulation of SRGAP1 by miR-340 and miR-124 was confirmed by western blot, dual luciferase activity assays and rescue experiments...
December 13, 2017: Oncogene
https://www.readbyqxmd.com/read/29233905/neddylation-blockade-diminishes-hepatic-metastasis-by-dampening-cancer-stem-like-cells-and-angiogenesis-in-uveal-melanoma
#6
Yanli Jin, Ping Zhang, Yun Wang, Bei Jin, Jingfeng Zhou, Jing Zhang, Jingxuan Pan
PURPOSE: Liver metastasis is the major and direct cause of death in patients with uveal melanoma (UM). There is no effective therapy for patients with metastatic UM. Improved treatments of hepatic metastatic patients with UM were urgently needed. Inspired by readily detectable key components in neddylation pathway in UM cells, we aimed at exploring whether neddylation pathway was a therapeutic target for liver metastatic UM. EXPERIMENTAL DESIGN: Expression of key proteins in neddylation pathway in UM was detected by Western blotting, real-time quantitative RT-PCR (qRT-PCR), and immunohistochemical staining...
December 12, 2017: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/29233795/using-microsimulation-to-estimate-the-future-health-and-economic-costs-of-salmonellosis-under-climate-change-in-central-queensland-australia
#7
Dimity Maree Stephen, Adrian Gerard Barnett
BACKGROUND: The incidence of salmonellosis, a costly foodborne disease, is rising in Australia. Salmonellosis increases during high temperatures and rainfall, and future incidence is likely to rise under climate change. Allocating funding to preventative strategies would be best informed by accurate estimates of salmonellosis costs under climate change and by knowing which population subgroups will be most affected. OBJECTIVE: We used microsimulation models to estimate the health and economic costs of salmonellosis in Central Queensland under climate change between 2016 and 2036 to inform preventative strategies...
December 11, 2017: Environmental Health Perspectives
https://www.readbyqxmd.com/read/29232894/significant-down-regulation-of-biological-adhesion-genes-in-porcine-oocytes-after-ivm
#8
Joanna Budna, Piotr Celichowski, Artur Bryja, Marta Dyszkiewicz-Konwińska, Michal Jeseta, Dorota Bukowska, Paweł Antosik, Klaus Peter Brüssow, Małgorzata Bruska, Michał Nowicki, Maciej Zabel, Bartosz Kempisty
Proper maturation of the mammalian oocyte is a compound processes determining successful monospermic fertilization, however the number of fully mature porcine oocytes is still unsatisfactory. Since oocytes' maturation and fertilization involve cellular adhesion and membranous contact, the aim was to investigate cell adhesion ontology group in porcine oocytes. The oocytes were collected from ovaries of 45 pubertal crossbred Landrace gilts and subjected to two BCB tests. After the first test, only granulosa cell-free BCB⁺ oocytes were directly exposed to microarray assays and RT-qPCR ("before IVM" group), or first in vitro matured and then if classified as BCB⁺ passed to molecular analyses ("after IVM" group)...
December 11, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29231262/mir-24-3p-promotes-cell-migration-and-proliferation-in-lung-cancer-by-targeting-sox7
#9
Liang Yan, Jinzhu Ma, Yiping Zhu, Jiawei Zan, Zhen Wang, Liefeng Ling, Qiang Li, Jun Lv, Shimei Qi, Yingya Cao, Ying Liu, Long Cao, Yao Zhang, Zhilin Qi, Liuwang Nie
Lung cancer (LC) is one of the leading causes of cancer-related death in the world. miR-24-3p plays critical roles in many cancer types, including LC. In this study, we first investigated whether miR-24-3p promoted LC cell migration and proliferation in vitro. We used three bioinformatics algorithms to predict the miR-24-3p target gene to study the molecular mechanism by which miR-24-3p contributes to LC progression. Then, we used the luciferase reporter assay to identify whether SOX7 was a direct target of miR-24-3p...
December 12, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29230879/microrna-143-5p-regulates-alpaca-melanocyte-migration-proliferation-and-melanogenesis
#10
Kaiyuan Ji, Pengqian Zhang, Junzhen Zhang, Ruiwen Fan, Yu Liu, Shanshan Yang, Shuaipeng Hu, Xuexian Liu, Changsheng Dong
microRNAs (miRNAs) have been shown to be closely involved in the control of melanogenesis and hair colour in mammals. Previous data also indicate that miR-143 regulates cell growth in melanoma. Here, we aimed to investigate the role of miR-143-5p in alpaca melanocytes. We found that miR-143-5p was highly expressed in the cytoplasm of alpaca melanocytes as demonstrated by an in situ hybridization assay. Prediction analysis revealed that miR-143-5p could regulate TGF-β-activated kinase 1 (TAK1) expression, which we confirmed by luciferase reporter assay, indicating that miR-143-5p controls TAK1 expression by directly targeting its 3' untranslated region (UTR)...
December 11, 2017: Experimental Dermatology
https://www.readbyqxmd.com/read/29229906/direction-of-actin-flow-dictates-integrin-lfa-1-orientation-during-leukocyte-migration
#11
Pontus Nordenfelt, Travis I Moore, Shalin B Mehta, Joseph Mathew Kalappurakkal, Vinay Swaminathan, Nobuyasu Koga, Talley J Lambert, David Baker, Jennifer C Waters, Rudolf Oldenbourg, Tomomi Tani, Satyajit Mayor, Clare M Waterman, Timothy A Springer
Integrin αβ heterodimer cell surface receptors mediate adhesive interactions that provide traction for cell migration. Here, we test whether the integrin, when engaged to an extracellular ligand and the cytoskeleton, adopts a specific orientation dictated by the direction of actin flow on the surface of migrating cells. We insert GFP into the rigid, ligand-binding head of the integrin, model with Rosetta the orientation of GFP and its transition dipole relative to the integrin head, and measure orientation with fluorescence polarization microscopy...
December 11, 2017: Nature Communications
https://www.readbyqxmd.com/read/29229885/mir-876-5p-suppresses-epithelial-mesenchymal-transition-of-lung-cancer-by-directly-down-regulating-bone-morphogenetic-protein-4
#12
Liang Bao, Lei Lv, Jinping Feng, Yuyu Chen, Xinhua Wang, Shuguang Han, Hongqing Zhao
Lung cancer is the leading cause of cancer-related death throughout the world. We aimed to investigate the role of a novel microRNA-876-5p and its potential molecular target bone morphogenetic protein 4 (BMP-4), in the epithelial-mesenchymal transition (EMT) of lung cancer. Expressions of microRNA-876-5p and its potential target BMP-4 were analysed in lung cancer cells and patient tissues. Luciferase activity assay was conducted to verify direct targeting of microRNA- 876-5p to the 3'-UTR of BMP-4 mRNA. Migration, invasion capacities of lung cancer cells expressing microRNA-876-5p were analysed, and characteristics of lung cancer EMT protein markers were also evaluated...
December 2017: Journal of Biosciences
https://www.readbyqxmd.com/read/29229852/regulatory-networks-specifying-cortical-interneurons-from-human-embryonic-stem-cells-reveal-roles-for-chd2-in-interneuron-development
#13
Kesavan Meganathan, Emily M A Lewis, Paul Gontarz, Shaopeng Liu, Edouard G Stanley, Andrew G Elefanty, James E Huettner, Bo Zhang, Kristen L Kroll
Cortical interneurons (cINs) modulate excitatory neuronal activity by providing local inhibition. During fetal development, several cIN subtypes derive from the medial ganglionic eminence (MGE), a transient ventral telencephalic structure. While altered cIN development contributes to neurodevelopmental disorders, the inaccessibility of human fetal brain tissue during development has hampered efforts to define molecular networks controlling this process. Here, we modified protocols for directed differentiation of human embryonic stem cells, obtaining efficient, accelerated production of MGE-like progenitors and MGE-derived cIN subtypes with the expected electrophysiological properties...
December 11, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29229584/eliminating-hepatitis-c-within-low-income-countries-the-need-to-cure-genotypes-4-5-6
#14
REVIEW
Tarik Asselah, Tarek Hassanein, Imam Waked, Abdellah Mansouri, Geoffrey Dusheiko, Edward Gane
Around 70 to 100 million people are chronically infected with HCV worldwide. HCV anti-viral drug development has been remarkable and this revolution led to several direct-acting antiviral agents achieving an HCV cure after only 8-12 weeks of treatment duration. Drug development has focused first on HCV genotype 1 infection, since it was the most prevalent worldwide, although clinical trials included all genotypes prevalent in the US and Europe. Because the earliest in vitro assays utilised the GT 1b and 2 replicons, the initial classes of direct acting antivirals (protease inhibitors, non-nucleotide polymerase inhibitors) were genotype 1-specific, albiet had an effect on other less prevalent genotypes...
December 8, 2017: Journal of Hepatology
https://www.readbyqxmd.com/read/29228717/somatic-prdm2-c-4467dela-mutations-in-colorectal-cancers-control-histone-methylation-and-tumor-growth
#15
Tatjana Pandzic, Veronica Rendo, Jinyeong Lim, Chatarina Larsson, Jimmy Larsson, Ivaylo Stoimenov, Snehangshu Kundu, Muhammad Akhtar Ali, Mats Hellström, Liqun He, Anders M Lindroth, Tobias Sjöblom
The chromatin modifier PRDM2/RIZ1 is inactivated by mutation in several forms of cancer and is a putative tumor suppressor gene. Frameshift mutations in the C-terminal region of PRDM2, affecting (A)8 or (A)9 repeats within exon 8, are found in one third of colorectal cancers with microsatellite instability, but the contribution of these mutations to colorectal tumorigenesis is unknown. To model somatic mutations in microsatellite unstable tumors, we devised a general approach to perform genome editing while stabilizing the mutated nucleotide repeat...
November 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/29228608/expression-of-mir-195-is-associated-with-chemotherapy-sensitivity-of-cisplatin-and-clinical-prognosis-in-gastric-cancer
#16
Rui Ye, Bo Wei, Sheng Li, Wei Liu, Juntao Liu, Luan Qiu, Xuan Wu, Zhifei Zhao, Jianxiong Li
Gastric cancer has higher morbidity and mortality than other cancers for the low diagnosis rate and few therapies. MiR-195 has been reported to be involved in the occurrence, development and prognosis of various cancers. However, the function of miR-195 in gastric cancer remains largely unknown. Herein, the aims of this study were to probe the functional mechanism of miR-195 and its chemotherapy sensitivity as well as clinical prognosis in gastric cancer. We screened out low-expressed miR-195 through microarray analysis and further confirmed miR-195 was widely down-regulated in gastric cancer cells...
November 14, 2017: Oncotarget
https://www.readbyqxmd.com/read/29228421/mir-377-suppresses-cell-proliferation-and-metastasis-in-gastric-cancer-via-repressing-the-expression-of-vegfa
#17
C-Q Wang, L Chen, C-L Dong, Y Song, Z-P Shen, W-M Shen, X-D Wu
OBJECTIVE: In recent years, microRNAs have been identified to participate in tumor genesis and progression of different tumors including gastric cancer. However, the role of miR-377 played in gastric cancer (GC), and its mechanisms have not been demonstrated. PATIENTS AND METHODS: We detected miR-377 expression level in 86 GC and adjacent normal tissue samples by quantitative reverse transcription PCR (qRT-PCR) as well as in GC cell lines. The relationship between miR-377 and clinical pathological features was analyzed...
November 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29226950/syndecans-in-chronic-inflammatory-and-autoimmune-diseases-pathological-insights-and-therapeutic-opportunities
#18
REVIEW
Solomon A Agere, Eugene Y Kim, Nahid Akhtar, Salahuddin Ahmed
Syndecans (SDCs) are a family of heparan sulfate proteoglycans (HSPGs) glycoproteins ubiquitously expressed on the cell surfaces and extracellular matrix of all mammalian tissues. There are four mammalian syndecans, SDC-1 thorough 4, which play a critical role in cell adhesion, migration, proliferation, differentiation, and angiogenesis through independent and growth factor mediated signaling. An altered expression of SDCs is often observed in autoimmune disorders, cancer, HIV infection, and many other pathological conditions...
December 11, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29226860/a-hybrid-multiscale-model-for-investigating-tumor-angiogenesis-and-its-response-to%C3%A2-cell-based-therapy
#19
Melisa Hendrata, Janti Sudiono
Angiogenesis, a formation of blood vessels from an existing vasculature, plays a key role in tumor growth and its progression into cancer. The lining of blood vessels consists of endothelial cells (ECs) which proliferate and migrate, allowing the capillaries to sprout towards the tumor to deliver the needed oxygen. Various treatments aiming to suppress or even inhibit angiogenesis have been explored. Mesenchymal stem cells (MSCs) have recently been undergoing development in cell-based therapy for cancer due to their ability to migrate towards the capillaries and induce the apoptosis of the ECs, causing capillary degeneration...
December 5, 2017: In Silico Biology
https://www.readbyqxmd.com/read/29226323/cops5-and-lasp1-synergistically-interact-to-down-regulate-14-3-3%C3%AF-expression-and-promote-colorectal-cancer-progression-via-activating-pi3k-akt-pathway
#20
Rui Zhou, Ziyun Shao, Jian Liu, Wanqi Zhan, Qingzu Gao, Zhihua Pan, Ling Wu, Lijun Xu, Yanqing Ding, Liang Zhao
Overexpression of LIM and SH3 protein 1 (LASP1) is required for colorectal cancer (CRC) development and progression. Here, C-Jun activation domain-binding protein-1 (Jab1), also known as COPS5, was verified as a new LASP1-interacting protein through yeast two-hybrid assay. The role of COPS5 in LASP1-mediated CRC progression remains unknown. GST pull-down assay indicated that the SH3 domain of LASP1 could directly bind to MPN domain of COPS5. In vitro gain- and loss-of-function analyses revealed the stimulatory role of COPS5 on CRC cell proliferation, migration and invasion...
December 11, 2017: International Journal of Cancer. Journal International du Cancer
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