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https://www.readbyqxmd.com/read/28816507/arthroscopic-harvest-of-adipose-derived-mesenchymal-stem-cells-from-the-infrapatellar-fat-pad
#1
Jason L Dragoo, Wenteh Chang
BACKGROUND: The successful isolation of adipose-derived mesenchymal stem cells (ADSCs) from the arthroscopically harvested infrapatellar fat pad (IFP) would provide orthopaedic surgeons with an autologous solution for regenerative procedures. PURPOSE: To demonstrate the quantity and viability of the mesenchymal stem cell population arthroscopically harvested from the IFP as well as the surrounding synovium. STUDY DESIGN: Descriptive laboratory study...
August 1, 2017: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/28814514/ba71%C3%AE-cd2-a-new-recombinant-live-attenuated-african-swine-fever-virus-with-cross-protective-capabilities
#2
Paula L Monteagudo, Anna Lacasta, Elisabeth López, Laia Bosch, Javier Collado, Sonia Pina-Pedrero, Florencia Correa-Fiz, Francesc Accensi, María Jesús Navas, Enric Vidal, María J Bustos, Javier M Rodríguez, Andreas Gallei, Veljko Nikolin, María L Salas, Fernando Rodríguez
African swine fever is a highly contagious viral disease of mandatory declaration to the World Organization for Animal Health (OIE). Lack of available vaccines make its control difficult and thus ASFV represents a major threat to the swine industry. Inactivated vaccines do not confer solid protection against African swine fever virus (ASFV). Conversely, live attenuated viruses (LAV), either naturally isolated or obtained by genetic manipulation, have demonstrated reliable protection against homologous ASFV strains, albeit little or no protection has been demonstrated against heterologous viruses...
August 16, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28813122/morphological-alterations-in-the-jejunal-mucosa-of-aged-rats-and-the-possible-protective-role-of-green-tea
#3
Zeinab Abel-Rehim Hassan, Agata Zauszkiewicz-Pawlak, Shaimaa A Abdelrahman, Sami Algaidi, Maha Desouky, Sally M Shalaby
INTRODUCTION: Gastrointestinal disorders become more prevalent with ageing. This study is aimed to describe morphological changes that occur in the jejunal mucosa of male albino rats as a result of ageing and the protective effect of green tea supplements. MATERIAL AND METHODS: The experiment was performed on sixty rats: thirty young-adult (6-month old, body mass 200-220 g) and thirty old (24-month-old, body mass 220-260 g) animals. Each group was further divided into two subgroups (n = 15 each): control rats and green tea-treated rats that received 1...
August 16, 2017: Folia Histochemica et Cytobiologica
https://www.readbyqxmd.com/read/28811255/cortical-layers-cyto-myelo-receptor-and-synaptic-architecture-in-human-cortical-areas
#4
REVIEW
Nicola Palomero-Gallagher, Karl Zilles
Cortical layers have classically been identified by their distinctive and prevailing cell types and sizes, as well as the packing densities of cell bodies or myelinated fibers. The densities of multiple receptors for classical neurotransmitters also vary across the depth of the cortical ribbon, and thus determine the neurochemical properties of cyto- and myeloarchitectonic layers. However, a systematic comparison of the correlations between these histologically definable layers and the laminar distribution of transmitter receptors is currently lacking...
August 12, 2017: NeuroImage
https://www.readbyqxmd.com/read/28810260/cytoplasmic-lipid-accumulation-characteristic-of-the-cribriform-variant-of-papillary-thyroid-carcinoma
#5
Nami Takada, Mitsuyoshi Hirokawa, Aki Ito, Ayana Suzuki, Miyoko Higuchi, Seiji Kuma, Toshitetsu Hayashi, Tsutomu Daa, Akira Miyauchi
OBJECTIVE: The purpose of this study was to clarify the diagnostic significance of cytoplasmic lipid accumulation (CLIA) in the cribriform variant of papillary thyroid carcinoma (CV-PTC). METHODS: We performed a histological, immunohistochemical, and cytological examination of 35 CV-PTC cases at the Kuma Hospital. CLIA was defined as bubble-like multivacuolation in cytoplasm with distinct cell border. We also examined 100 conventional PTC (con-PTC) cases as controls...
August 16, 2017: Pathobiology: Journal of Immunopathology, Molecular and Cellular Biology
https://www.readbyqxmd.com/read/28809777/pigmented-epithelioid-melanocytoma-pem-animal-type-melanoma-atm-quest-for-an-origin-report-of-one-unusual-case-indicating-follicular-origin-and-another-arising-in-an-intradermal-nevus
#6
Ashley Tarasen, J Andrew Carlson, M Kathryn Leonard, Glenn Merlino, David Kaetzel, Andrzej T Slominski
Pigmented epithelioid melanocytoma (PEM) is a tumor encompassing epithelioid blue nevus of Carney complex (EBN of CNC) and was previously termed animal-type melanoma. Histologically PEMs are heavily pigmented spindled and epithelioid dermal melanocytic tumors with infiltrative borders, however, their origin remains unclear. Stem cells for the epidermis and hair follicle are located in the bulge area of the hair follicle with the potential to differentiate into multiple lineages. Multiple cutaneous carcinomas, including follicular cutaneous squamous cell carcinoma (FSCC), are thought to arise from stem cells in the follicular bulge...
August 15, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28808411/preoperative-factors-associated-with-infiltrative-histologic-growth-patterns-in-extremity-soft-tissue-sarcoma
#7
Jong Woong Park, Han-Soo Kim, Cheol Lee, Hye Jin Yoo, Ji Yeon Yun, Ilkyu Han
Soft tissue sarcoma (STS) with an infiltrative histologic growth pattern, when compared to STS with an expansile pattern, may pose difficulties in local control. Preoperative assessment of the presence of infiltrative histologic growth pattern would be helpful in deciding treatment strategies. A review of 144 patients who underwent surgery for extremity STS was performed. Microscopically, the histologic growth pattern was defined as infiltrative if the penetration of the tumor cells into the surrounding tissue was observed...
2017: Sarcoma
https://www.readbyqxmd.com/read/28804725/meprin-metalloprotease-deficiency-associated-with-higher-mortality-rates-and-more-severe-diabetic-kidney-injury-in-mice-with-stz-induced-type-1-diabetes
#8
John E Bylander, Faihaa Ahmed, Sabena M Conley, Jean-Marie Mwiza, Elimelda Moige Ongeri
Meprins are membrane-bound and secreted metalloproteinases consisting of α and/or β subunits that are highly expressed in kidney epithelial cells and are differentially expressed in podocytes and leukocytes (macrophages and monocytes). Several studies have implicated meprins in the progression of diabetic nephropathy (DN) and fibrosis-associated kidney disease. However, the mechanisms by which meprins modulate DN are not understood. To delineate the role of meprins in DN, we subjected meprin αβ knockout (αβKO) mice and their wild-type (WT) counterparts to streptozotocin-induced type 1 diabetes...
2017: Journal of Diabetes Research
https://www.readbyqxmd.com/read/28798746/automated-analysis-of-flow-cytometry-data-to-reduce-inter-lab-variation-in-the-detection-of-major-histocompatibility-complex-multimer-binding-t-cells
#9
Natasja Wulff Pedersen, P Anoop Chandran, Yu Qian, Jonathan Rebhahn, Nadia Viborg Petersen, Mathilde Dalsgaard Hoff, Scott White, Alexandra J Lee, Rick Stanton, Charlotte Halgreen, Kivin Jakobsen, Tim Mosmann, Cécile Gouttefangeas, Cliburn Chan, Richard H Scheuermann, Sine Reker Hadrup
Manual analysis of flow cytometry data and subjective gate-border decisions taken by individuals continue to be a source of variation in the assessment of antigen-specific T cells when comparing data across laboratories, and also over time in individual labs. Therefore, strategies to provide automated analysis of major histocompatibility complex (MHC) multimer-binding T cells represent an attractive solution to decrease subjectivity and technical variation. The challenge of using an automated analysis approach is that MHC multimer-binding T cell populations are often rare and therefore difficult to detect...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28795790/borders-and-beyond
#10
EDITORIAL
P B Persson, Anja Bondke Persson
Compartmentalization is a basic requirement of life on earth. Eukaryotic cells separate themselves from their surroundings by biological membranes which function as intricately regulated barriers. These borders, at best, allow e.g. intercellular communication and transport. They ensure that intruders are kept outside, that supplies are coming in and products are exported and that waste is got rid of, all in due time and amount. Cell membranes protect the cell and organize its functionality. That is a far as the ideal situation goes...
August 10, 2017: Acta Physiologica
https://www.readbyqxmd.com/read/28794894/swine-enteric-colibacillosis-diagnosis-therapy-and-antimicrobial-resistance
#11
REVIEW
Andrea Luppi
Intestinal infection with enterotoxigenic Escherichia coli (ETEC) is an important disease in swine resulting in significant economic losses. Knowledge about the epidemiology, the diagnostic approach and methods of control are of fundamental importance to tackle the disease. The ETEC causing neonatal colibacillosis mostly carry the fimbriae F4 (k88), F5 (k99), F6 (987P) or F41, while the ETEC of post-weaning diarrhoea carry the fimbriae F4 (k88) and F18. These fimbriae adhere to specific receptors on porcine intestinal brush border epithelial cells (enterocytes), starting the process of enteric infection...
2017: Porcine Health Management
https://www.readbyqxmd.com/read/28792434/collective-migration-of-lens-epithelial-cell-induced-by-differential-microscale-groove-patterns
#12
Chunga Kwon, Youngjun Kim, Hojeong Jeon
Herein, a micro-patterned cell adhesive surface is prepared for the future design of medical devices. One-dimensional polydimethylsiloxane (PDMS) micro patterns were prepared by a photolithography process. We investigated the effect of microscale topographical patterned surfaces on decreasing the collective cell migration rate. PDMS substrates were prepared through soft lithography using Si molds fabricated by photolithography. Afterwards, we observed the collective cell migration of human lens epithelial cells (B-3) on various groove/ridge patterns and evaluated the migration rate to determine the pattern most effective in slowing down the cell sheet spreading speed...
August 9, 2017: Journal of Functional Biomaterials
https://www.readbyqxmd.com/read/28789587/light-on-a-sensory-interface-linking-the-cerebrospinal-fluid-to-motor-circuits-in-vertebrates
#13
Lydia Djenoune, Claire Wyart
The cerebrospinal fluid (CSF) is circulating around the entire central nervous system (CNS). The main function of the CSF has been thought to insure the global homeostasis of the CNS. Recent evidence indicates that the CSF also dynamically conveys signals modulating the development and the activity of the nervous system. The later observation implies that cues from the CSF could act on neurons in the brain and the spinal cord via bordering receptor cells. Candidate neurons to enable such modulation are the cerebrospinal fluid-contacting neurons (CSF-cNs) that are located precisely at the interface between the CSF and neuronal circuits...
August 8, 2017: Journal of Neurogenetics
https://www.readbyqxmd.com/read/28783176/short-stretches-of-rare-codons-regulate-translation-of-the-transcription-factor-zeb2-in-cancer-cells
#14
W R Wan Makhtar, G Browne, A Karountzos, C Stevens, Y Alghamdi, A R Bottrill, S Mistry, E Smith, M Bushel, J H Pringle, A E Sayan, E Tulchinsky
Two proteins comprising the ZEB family of zinc finger transcription factors, ZEB1 and ZEB2, execute EMT programs in embryonic development and cancer. By studying regulation of their expression, we describe a novel mechanism that limits ZEB2 protein synthesis. A protein motif located at the border of the SMAD-binding domain of ZEB2 protein induces ribosomal pausing and compromises protein synthesis. The function of this protein motif is dependent on stretches of rare codons, Leu(UUA)-Gly(GGU)-Val(GUA). Incorporation of these triplets in the homologous region of ZEB1 does not affect protein translation...
August 7, 2017: Oncogene
https://www.readbyqxmd.com/read/28774379/fibrogenic-potential-of-pw1-peg3-expressing-cardiac-stem-cells
#15
Elisa Yaniz-Galende, Maguelonne Roux, Sophie Nadaud, Nathalie Mougenot, Marion Bouvet, Olivier Claude, Guillaume Lebreton, Catherine Blanc, Florence Pinet, Fabrice Atassi, Claire Perret, France Dierick, Sébastien Dussaud, Pascal Leprince, David-Alexandre Trégouët, Giovanna Marazzi, David Sassoon, Jean-Sébastien Hulot
BACKGROUND: Pw1 gene expression is a marker of adult stem cells in a wide range of tissues. PW1-expressing cells are detected in the heart but are not well characterized. OBJECTIVES: The authors characterized cardiac PW1-expressing cells and their cell fate potentials in normal hearts and during cardiac remodeling following myocardial infarction (MI). METHODS: A human cardiac sample was obtained from a patient presenting with reduced left ventricular (LV) function following a recent MI...
August 8, 2017: Journal of the American College of Cardiology
https://www.readbyqxmd.com/read/28771713/epa-blocks-tnf-%C3%AE-induced-inhibition-of-sugar-uptake-in-caco-2-cells-via-gpr120-and-ampk
#16
Rosa Castilla-Madrigal, Jaione Barrenetxe, María J Moreno-Aliaga, María Pilar Lostao
The aim of the present work was to investigate in Caco-2 cells whether eicosapentaenoic acid (EPA), an omega-3 polyunsaturated fatty acid, could block the inhibitory effect of tumor necrosis factor-α (TNF-α) on sugar transport, and identify the intracellular signaling pathways involved. After pre-incubation of the Caco-2 cells with TNF-α and EPA for 1 h, EPA prevented the inhibitory effect of the cytokine on α-methyl-D-glucose (αMG) uptake (15 min) and on SGLT1 expression at the brush border membrane, measured by Western blot...
August 3, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28770162/squamous-cell-carcinoma-of-the-buccal-mucosa-involving-the-masticator-space-a-case-report
#17
Il-Hyung Kim, Hoon Myoung
Squamous cell carcinoma of the buccal mucosa has an aggressive nature, as it grows rapidly and penetrates well with a high recurrence rate. If cancers originating from the buccal mucosa invade adjacent anatomical structures, surgical tumor resection becomes more challenging, thus raising specific considerations for reconstruction relative to the extent of resection. The present case describes the surgical management of a 58-year-old man who presented with persistent ulceration of the mucosal membrane and a mouth-opening limitation of 11 mm...
June 2017: Journal of the Korean Association of Oral and Maxillofacial Surgeons
https://www.readbyqxmd.com/read/28769779/size-matters-how-scaling-affects-the-interaction-between-grid-and-border-cells
#18
Diogo Santos-Pata, Riccardo Zucca, Sock C Low, Paul F M J Verschure
Many hippocampal cell types are characterized by a progressive increase in scale along the dorsal-to-ventral axis, such as in the cases of head-direction, grid and place cells. Also located in the medial entorhinal cortex (MEC), border cells would be expected to benefit from such scale modulations. However, this phenomenon has not been experimentally observed. Grid cells in the MEC of mammals integrate velocity related signals to map the environment with characteristic hexagonal tessellation patterns. Due to the noisy nature of these input signals, path integration processes tend to accumulate errors as animals explore the environment, leading to a loss of grid-like activity...
2017: Frontiers in Computational Neuroscience
https://www.readbyqxmd.com/read/28766977/surgical-management-of-supraglottic-stenosis-using-intubationless-optiflow
#19
Kenric Tam, Caroline Jeffery, C Kwang Sung
OBJECTIVE: Airway management during endoscopic surgical treatment of supraglottic and pharyngeal stenosis is often challenging and can be accomplished by various means, including tracheostomy, jet ventilation, or direct laryngoscopy. We describe CO2 laser excision of supraglottic-pharyngeal stenosis using intubationless Optiflow high-flow nasal cannula (HFNC). METHODS: A 55-year-old male presented with dyspnea and dysphagia secondary to severe supraglottic-pharyngeal stenosis in the setting of previous chemoradiation for a T0N2aM0 squamous cell carcinoma...
September 2017: Annals of Otology, Rhinology, and Laryngology
https://www.readbyqxmd.com/read/28764952/prospective-comparison-of-18-f-naf-pet-ct-versus-18-f-fdg-pet-ct-imaging-in-mandibular-extension-of-head-and-neck-squamous-cell-carcinoma-with-dedicated-analysis-software-and-validation-with-surgical-specimen-a-preliminary-study
#20
Raphael Lopez, Pierre Gantet, Anne Sophie Salabert, Anne Julian, Anne Hitzel, Beatrice Herbault-Barres, Charlotte Fontan, Sarah Alshehri, Pierre Payoux
BACKGROUND: The aim of this study is to propose a new method to quantify radioactivity with PET/CT imaging in mandibular extension in head and neck squamous cell carcinoma (HNSCC), using innovative software, and to compare results with microscopic surgical specimens. PATIENTS AND METHODS: This prospective study enrolled 15 patients who underwent (18)F-NaF and (18)F-FDG PET/CT. We compared the delineations of bone invasions obtained with (18)F-NaF PET/CT and (18)F-FDG PET/CT with the results of histopathological analysis of mandibular resections (from right and left bone borders)...
July 8, 2017: Journal of Cranio-maxillo-facial Surgery
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