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https://www.readbyqxmd.com/read/28231690/downregulation-of-hur-inhibits-the-progression-of-esophageal-cancer-through-interleukin-18
#1
Xiaohui Xu, Cheng Song, Zhihua Chen, Chenxiao Yu, Yi Wang, Yiting Tang, Judong Luo
Purpose: To investigate the effect of HuR downregulation and the potential target genes of HuR on the progression of esophageal squamous cell carcinoma (ESCC). Materials and Methods: In this study, a proteomics assay was used to detect the expression of proteins after HuR downregulation, and a luciferase assay was used to detect the potential presence of a HuR binding site on the 3'-UTR of IL-18. In addition, colony formation assay, MTT, EdU incorporation assay, Western blot, flow cytometry, immunohistochemistry, transwell invasion assay and wound healing assay were used...
February 24, 2017: Cancer Research and Treatment: Official Journal of Korean Cancer Association
https://www.readbyqxmd.com/read/28230093/pentabromophenol-suppresses-tgf-%C3%AE-signaling-by-accelerating-degradation-of-type-ii-tgf-%C3%AE-receptors-via-caveolae-mediated-endocytosis
#2
Chun-Lin Chen, Pei-Hua Yang, Yu-Chen Kao, Pei-Yu Chen, Chih-Ling Chung, Shih-Wei Wang
Pentabromophenol (PBP), a brominated flame retardant (BFR), is widely used in various consumer products. BFRs exert adverse health effects such as neurotoxic and endocrine-disrupting effects. In this study, we found that PBP suppressed TGF-β response by accelerating the turnover rate of TGF-β receptors. PBP suppressed TGF-β-mediated cell migration, PAI-1 promoter-driven reporter gene activation, and Smad2/3 phosphorylation in various cell types. Furthermore, PBP abolished TGF-β-mediated repression of E-cadherin expression, in addition to the induction of vimentin expression and N-cadherin and fibronectin upregulation, thus blocking TGF-β-induced epithelial-mesenchymal transition in A549 and NMuMG cells...
February 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28229930/atrial-natriuretic-peptide-a-novel-mediator-for-tgf-%C3%AE-1-induced-epithelial-mesenchymal-transition-in-16hbe-14o-and-a549-cells
#3
Shuyuan Chu, Xiufeng Zhang, Yabing Sun, Yuanyuan Yu, Yaxi Liang, Ming Jiang, Jianwei Huang, Libing Ma
Atrial natriuretic peptide (ANP) is increasingly expressed on airway and inhibits pulmonary arterial remodeling. However, the role of ANP in remodeling of respiratory system is still unclear. The role of ANP on airway remodeling and the possible mechanism was explored in this study. Both human bronchial epithelial 16HBE-14o cells and alveolar epithelial A549 cells were stimulated by TGF-β1, ANP, cGMP inhibitor, PKG inhibitor, and cGMP analogue. The expressions of epithelial markers, mesenchymal markers, and Smad3 were assessed by quantitative real-time PCR and western blotting...
February 13, 2017: Peptides
https://www.readbyqxmd.com/read/28228550/interaction-of-phosphorylated-rab11-fip2-with-eps15-regulates-apical-junction-composition
#4
Lynne A Lapierre, Elizabeth H Manning, Kenya M Mitchell, Cathy M Caldwell, James R Goldenring
MARK2 regulates the establishment of polarity in MDCK cells in part through phosphorylation of serine 227 of Rab11-FIP2. We have now identified Eps15 as an interacting partner of phospho-S227-Rab11-FIP2 (pS227-FIP2). During recovery from low calcium, Eps15 localized to the lateral membrane, prior to pS227-FIP2 arrival. Later in recovery, Eps15 and pS227-FIP2 co-localized at the lateral membrane. In MDCK cells expressing the pseudophosphorylated FIP2 mutant, FIP2(S227E), during recovery from low calcium, Eps15 was trapped and never localized to the lateral membrane...
February 22, 2017: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/28225782/chronic-treatment-of-non-small-cell-lung-cancer-cells-with-gefitinib-leads-to-an-epigenetic-loss-of-epithelial-properties-associated-with-reductions-in-microrna-155-and-200c
#5
Michiko Narita, Eri Shimura, Atsumi Nagasawa, Toshiki Aiuchi, Yukari Suda, Yusuke Hamada, Daigo Ikegami, Chizuru Iwasawa, Kazuhiko Arakawa, Katsuhide Igarashi, Naoko Kuzumaki, Yusuke Yoshioka, Takahiro Ochiya, Hideyuki Takeshima, Toshikazu Ushijima, Minoru Narita
BACKGROUND: The EGFR tyrosine kinase inhibitor gefitinib is used in therapy for non-small-cell lung cancer (NSCLC). However, its application is limited by resistance-accelerated disease progression, which is accompanied by the epithelial-to-mesenchymal transition (EMT). In the present study, we performed multiple expression analyses of microRNAs (miRNAs) and quantified the expression of several related EMT players in gefitinib-resistant NSCLC cells. METHODS AND RESULTS: To establish gefitinib-resistant NSCLC cells, gefitinib-sensitive HCC827 cells, which exhibit an in-frame deletion [E746-A750] in EGFR exon 19, were exposed to gefitinib for at least 1...
2017: PloS One
https://www.readbyqxmd.com/read/28225561/flotillins-control-zebrafish-epiboly-through-their-role-in-cadherin-mediated-cell-cell-adhesion
#6
Eduardo A Rios Morris, Stéphane Bodin, Bénédicte Delaval, Franck Comunale, Virginie Georget, Manoel L Costa, Georges Lutfalla, Cécile Gauthier-Rouvière
Zebrafish gastrulation and particularly epiboly that involves coordinated movements of several cell layers is a dynamic process for which regulators remain to be identified. We show here that Flotillin 1 and 2, ubiquitous and highly conserved proteins, are required for epiboly. Flotillins knockdown compromised embryo survival, strongly delayed epiboly and impaired deep cell radial intercalation and directed collective migration without affecting enveloping layer cell movement. At the molecular level, we identified that Flotillins are required for the formation of E-cadherin-mediated cell-cell junctions...
February 22, 2017: Biology of the Cell
https://www.readbyqxmd.com/read/28223829/cul4a-promotes-proliferation-and-metastasis-of-colorectal-cancer-cells-by-regulating-h3k4-trimethylation-in-epithelial-mesenchymal-transition
#7
Xuemei Sui, Hong Zhou, Lei Zhu, Deqiang Wang, Sumei Fan, Wei Zhao
Increasing evidence suggests that CUL4A, a ubiquitin ligase, is involved in the promotion of cancer malignancy and correlated with worse clinical prognosis in several kinds of human cancers. Although its effect and mechanism on the progression of colorectal cancer (CRC) remain unknown. Our clinical data show that CUL4A protein is overexpressed, positively associated with lymph nodes status, differentiation degree, tumor size, and poor prognosis in 80 CRC patients. CUL4A overexpression promotes cell proliferation and colony formation of CRC cells...
2017: OncoTargets and Therapy
https://www.readbyqxmd.com/read/28223537/reduced-e-cadherin-facilitates-renal-cell-carcinoma-progression-by-wnt-%C3%AE-catenin-signaling-activation
#8
Xinqi Zhang, Mingxi Yang, Hua Shi, Jianxin Hu, Yuanlin Wang, Zhaolin Sun, Shuxiong Xu
Reduced expression of E-cadherin was observed in renal cell carcinoma (RCC). However, its potential clinical value and correlation with WNT/β-catenin signaling in RCC progression was still unclear. Immunohistochemical staining was performed in RCC tissue microarray to examine the expression status and prognosis value of E-cadherin and β-catenin. The potential role of E-cadherin in β-catenin translocation was analyzed with immunobloting assays. A significant negative correlation was observed between E-cadherin and β-catenin expression in RCC tissues...
February 15, 2017: Oncotarget
https://www.readbyqxmd.com/read/28220242/expression-of-clonorchis-sinensis-giiispla2-protein-in-baculovirus-infected-insect-cells-and-its-overexpression-facilitating-epithelial-mesenchymal-transition-in-huh7-cells-via-akt-pathway
#9
Mei Shang, Zhizhi Xie, Zeli Tang, Lei He, Xiaoyun Wang, Caiqin Wang, Yinjuan Wu, Ye Li, Lu Zhao, Zhiyue Lv, Zhongdao Wu, Yan Huang, Xinbing Yu, Xuerong Li
Although prior studies confirmed that group III secretory phospholipase A2 of Clonorchis sinensis (CsGIIIsPLA2) had stimulating effect on liver fibrosis by binding to LX-2 cells, large-scale expression of recombinant protein and its function in the progression of hepatoma are worth exploring. Because of high productivity and low lipopolysaccharides (LPS) in the Sf9-baculovirus expression system, we firstly used this system to express the coding region of CsGIIIsPLA2. The molecular weight of recombinant CsGIIIsPLA2 protein was about 34 kDa...
February 20, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28219947/phosphorylation-potential-of-drosophila-e-cadherin-intracellular-domain-is-essential-for-development-and-regulating-adherens-junction-biosynthetic-dynamics
#10
Yi-Jiun Chen, Juan Huang, Lynn Huang, Erin Austin, Yang Hong
E-Cadherin intracellular domain contains a highly conserved serine cluster whose phosphorylations are essential for binding to β-Catenin in vitro. In cultured cells phosphorylations of specific serine residues within the cluster are also required for regulating adherens junction (AJ) stability and dynamics. However, much less is known how such phosphorylations of E-Cadherin regulate the AJ formation and dynamics in vivo. In this report we generated an extensive array of Drosophila E-Cadherin (DE-Cad) endogenous knock-in alleles that carry mutations targeting this highly conserved serine cluster...
February 20, 2017: Development
https://www.readbyqxmd.com/read/28218910/a-mechanically-active-heterotypic-e-cadherin-n-cadherin-adhesion-enables-fibroblasts-to%C3%A2-drive-cancer-cell-invasion
#11
Anna Labernadie, Takuya Kato, Agustí Brugués, Xavier Serra-Picamal, Stefanie Derzsi, Esther Arwert, Anne Weston, Victor González-Tarragó, Alberto Elosegui-Artola, Lorenzo Albertazzi, Jordi Alcaraz, Pere Roca-Cusachs, Erik Sahai, Xavier Trepat
Cancer-associated fibroblasts (CAFs) promote tumour invasion and metastasis. We show that CAFs exert a physical force on cancer cells that enables their collective invasion. Force transmission is mediated by a heterophilic adhesion involving N-cadherin at the CAF membrane and E-cadherin at the cancer cell membrane. This adhesion is mechanically active; when subjected to force it triggers β-catenin recruitment and adhesion reinforcement dependent on α-catenin/vinculin interaction. Impairment of E-cadherin/N-cadherin adhesion abrogates the ability of CAFs to guide collective cell migration and blocks cancer cell invasion...
February 20, 2017: Nature Cell Biology
https://www.readbyqxmd.com/read/28217761/modeling-of-chronic-radiation-induced-cystitis-in-mice
#12
Bernadette M M Zwaans, Sarah Krueger, Sarah N Bartolone, Michael B Chancellor, Brian Marples, Laura E Lamb
PURPOSE: Radiation cystitis (RC), a severe inflammatory bladder condition, develops as a side-effect of pelvic radiation therapy in cancer patients. There are currently no effective therapies to treat RC, in part due to the lack of preclinical model systems. In this study, we developed a mouse model for RC and used a small animal radiation research platform (SARRP) to simulate the targeted delivery of radiation as used with human patients. METHODS AND MATERIALS: To induce RC, C3H mice received a single radiation dose of 20Gy delivered through two beams...
October 2016: Advances in Radiation Oncology
https://www.readbyqxmd.com/read/28216386/serotonin-disturbs-colon-epithelial-tolerance-of-commensal-e-coli-by-increasing-nox-2-derived-superoxide
#13
Suhrid Banskota, Sushil Chandra Regmi, Jaya Gautam, Pallavi Gurung, Yu-Jeong Lee, Sae Kwang Ku, Jin-Hyung Lee, Jintae Lee, Hyeun Wook Chang, Sang Joon Park, Jung-Ae Kim
Adherent-invasive E. coli colonization and Toll-like receptor (TLR) expression are increased in the gut of inflammatory bowel disease (IBD) patients. However, the underlying mechanism of such changes has not been determined. In the current study, it was examined whether gut serotonin (5-hydroxytryptamine, 5-HT) can induce adherent-invasive E. coli colonization and increase TLR expression. In a co-culture system, commensal E. coli strain (BW25113, BW) adhered minimally to colon epithelial cells, but this was significantly enhanced by 5-HT to the level of a pathogenic strain (EDL933)...
February 16, 2017: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/28216015/differential-regulation-of-nm23-h1-under-hypoxic-and-serum-starvation-conditions-in-metastatic-cancer-cells-and-its-implication-in-emt
#14
Reyaz Ur Rasool, Debasis Nayak, Souneek Chakraborty, Vijay Lakshmi Jamwal, Vidushi Mahajan, Archana Katoch, Mir Mohd Faheem, Zainab Iqra, Hina Amin, Sumit G Gandhi, Anindya Goswami
Multiple stresses are prevalent inside the tumor microenvironment rendering tumor growth, neighboring invasion and metastasis of the cancer cells to distant organs. NM23-H1 is the first metastasis suppressor gene identified and known to be implicated as an important regulator of stress-induced metastasis. Herein, we demonstrated that prototypical NM23-H1 expression diminished during hypoxia and serum starvation in Panc-1/MDA-MB-231 cells, but converse invasion patterns were obtained in these two diverse stresses...
February 4, 2017: European Journal of Cell Biology
https://www.readbyqxmd.com/read/28214216/cancer-stem-cell-cytokeratins-and-epithelial-to-mesenchymal-transition-markers-expression-in-oral-squamous-cell-carcinoma-derived-from-ortothopic-xenoimplantation-of-cd44-high-cells
#15
Nathália Paiva de Andrade, Maria Fernanda Setúbal Destro Rodrigues, Camila Oliveira Rodini, Fabio Daumas Nunes
Oral squamous cell carcinoma (OSCC) is the most prevalent neoplasia of oral cavity worldwide and prognosis remains unchanged in decades. Recently, different authors reported that head and neck squamous cell carcinomas have a subpopulation of tumor initiating cells that apparently correspond to cancer stem cells (CSC) and are also responsible for tumor growth and metastasis. The purpose of the present study was to investigate the microscopic and phenotypic characteristics of OSCC tumors induced after orthotopic xenoimplantation of SCC9(WT) cell line and CSC-enriched subpopulation isolated from SCC9 cell line based on high expression of the putative CSC marker CD44...
December 23, 2016: Pathology, Research and Practice
https://www.readbyqxmd.com/read/28214208/reduced-immunohistochemical-expression-of-adhesion-molecules-in-vitiligo-skin-biopsies
#16
Adriane Reichert Faria, Juliana Elizabeth Jung, Caio César Silva de Castro, Lucia de Noronha
Because defects in adhesion impairment seem to be involved in the etiopathogenesis of vitiligo, this study aimed to compare the immunohistochemical expression of several adhesion molecules in the epidermis of vitiligo and non lesional vitiligo skin. Sixty-six specimens of lesional and non lesional skin from 33 volunteers with vitiligo were evaluated by immunohistochemistry using anti-beta-catenin, anti-E-cadherin, anti-laminin, anti-beta1 integrin, anti-collagen IV, anti-ICAM-1 and anti-VCAM-1 antibodies. Biopsies of vitiligo skin demonstrated a significant reduction in the expression of laminin and integrin...
December 30, 2016: Pathology, Research and Practice
https://www.readbyqxmd.com/read/28212573/dynamic-variations-in-epithelial-to-mesenchymal-transition-emt-atm-and-slfn11-govern-response-to-parp-inhibitors-and-cisplatin-in-small-cell-lung-cancer
#17
C Allison Stewart, Pan Tong, Robert J Cardnell, Triparna Sen, Lerong Li, Carl M Gay, Fatemah Masrorpour, You Fan, Rasha O Bara, Ying Feng, Yuanbin Ru, Junya Fujimoto, Samrat T Kundu, Leonard E Post, Karen Yu, Yuqiao Shen, Bonnie S Glisson, Ignatio Wistuba, John V Heymach, Don L Gibbons, Jing Wang, Lauren Averett Byers
Small cell lung cancer (SCLC) is one of the most aggressive forms of cancer, with a 5-year survival <7%. A major barrier to progress is the absence of predictive biomarkers for chemotherapy and novel targeted agents such as PARP inhibitors. Using a high-throughput, integrated proteomic, transcriptomic, and genomic analysis of SCLC patient-derived xenografts (PDXs) and profiled cell lines, we identified biomarkers of drug sensitivity and determined their prevalence in patient tumors. In contrast to breast and ovarian cancer, PARP inhibitor response was not associated with mutations in homologous recombination (HR) genes (e...
February 15, 2017: Oncotarget
https://www.readbyqxmd.com/read/28212565/the-mir-675-5p-regulates-the-progression-and-development-of-pancreatic-cancer-via-the-ubqln1-zeb1-mir200-axis
#18
Jue Wang, Youli Zhang, Hong Wei, Xingxing Zhang, Yan Wu, Aihua Gong, Yu Xia, Wenbing Wang, Min Xu
Pancreatic cancer (PC) is a highly lethal disease due to extensive metastatic lesions. Accumulating evidence suggests that miR-675-5p plays different roles in metastasis through the regulation of epithelial to mesenchymal (EMT) and the mesenchymal to epithelial transitions (MET) in different cancers. ZEB1 promotes the EMT process by controlling the expression of E-cadherin and may have a reciprocal regulation with Ubiquilin1 (UBQLN1) and mir-200 family in cancer progression. In the present study, we showed that decreased expression of miR-675-5p is associated with the enhanced cell proliferation and survival of PC cells, while the increased expression of mir-675-5p shows the opposite one...
February 15, 2017: Oncotarget
https://www.readbyqxmd.com/read/28212558/ataxin-1-regulates-epithelial-mesenchymal-transition-of-cervical-cancer-cells
#19
A-Ram Kang, Hyoung-Tae An, Jesang Ko, Seongman Kang
The mutant form of the protein ataxin-1 (ATXN1) causes the neurodegenerative disease spinocerebellar ataxia type-1. Recently, ATXN1 was reported to enhance E-cadherin expression in the breast cancer cell line MCF-7, suggesting a potential association between ATXN1 and cancer development. In the present study, we discovered a novel mechanism through which ATXN1 regulates the epithelial-mesenchymal transition (EMT) of cancer cells. Hypoxia-induced upregulation of the Notch intracellular domain expression decreased ATXN1 expression via MDM2-associated ubiquitination and degradation...
February 14, 2017: Oncotarget
https://www.readbyqxmd.com/read/28212533/upregulation-of-long-non-coding-rna-plncrna-1-promotes-proliferation-and-induces-epithelial-mesenchymal-transition-in-prostate-cancer
#20
Yang Jin, Zilian Cui, Xudong Li, Xunbo Jin, Jian Peng
OBJECTIVE: To confirm that PlncRNA-1 regulates the cell cycle in prostate cancer cells and induces epithelial-mesenchymal transition (EMT) in prostate cancer through the TGF-β1 pathway. RESULTS: PlncRNA-1 and TGF-β1 expression levels were significantly higher in prostate cancer tissues than in normal prostate tissues (P < 0.05) and were significantly positively correlated. TGF-β1, N-cadherin and Cyclin-D1 were downregulated and E-Cadherin was upregulated in LNCAP cells after silencing of PlncRNA-1, as determined by real-time PCR and Western blot...
February 13, 2017: Oncotarget
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