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https://www.readbyqxmd.com/read/27930694/influence-of-fasting-status-and-sample-preparation-on-metabolic-biomarker-measurements-in-postmenopausal-women
#1
Neil Murphy, Roni T Falk, Diana B Messinger, Michael Pollak, Xiaonan Xue, Juan Lin, Robin Sgueglia, Howard D Strickler, Mia M Gaudet, Marc J Gunter
BACKGROUND: Epidemiologic data linking metabolic markers-such as insulin, insulin-like growth factors (IGFs)-and adipose tissue-derived factors with cancer are inconsistent. Between-study differences in blood collection protocols, in particular participant's fasting status, may influence measurements. METHODS: We investigated the impact of fasting status and blood sample processing time on components of the insulin/IGF axis and in adipokines in a controlled feeding study of 45 healthy postmenopausal-women aged 50-75 years...
2016: PloS One
https://www.readbyqxmd.com/read/27924537/targeting-aflatoxin-biosynthetic-genes
#2
Ali Y Srour, Ahmad M Fakhoury, Robert L Brown
Chemical detoxification and physical destruction of aflatoxins in foods and feed commodities are mostly unattainable in a way that preserves the edibility of the food. Therefore, preventing mycotoxins in general and aflatoxins in particular from entering the food chain is a better approach. This requires early detection of the aflatoxin-causing organisms. Detection and quantification of aflatoxin-producing fungi has always been a challenge, especially within species of Aspergillus and Penicillium. Culture-based methods require a high level of expertise and a list of sophisticated equipment...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27924535/targeting-conserved-genes-in-fusarium-species
#3
Jéssica Gil-Serna, Belén Patiño, Miguel Jurado, Salvador Mirete, Covadonga Vázquez, M Teresa González-Jaén
Fumonisins are important mycotoxins contaminating foods and feeds which are mainly produced by F. verticillioides and F. proliferatum. Additionally, both are pathogens of maize and other cereals. We describe two highly sensitive, rapid, and species-specific PCR protocols which enable detection and discrimination of these closely related species in cereal flour or grain samples. The specific primer pairs of these assays were based on the intergenic spacer region of the multicopy rDNA unit which highly improves the sensitivity of the PCR assay in comparison with single-copy target regions...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27920192/results-of-an-international-phosphorus-digestibility-ring-test-with-broiler-chickens
#4
M Rodehutscord, O Adeola, R Angel, P Bikker, E Delezie, W A Dozier, M Umar Faruk, M Francesch, C Kwakernaak, A Narcy, C M Nyachoti, O A Olukosi, A Preynat, B Renouf, A Saiz Del Barrio, K Schedle, W Siegert, S Steenfeldt, M M van Krimpen, S M Waititu, M Witzig
The objective of this ring test was to investigate the prececal phosphorus (P) digestibility of soybean meal (SBM) in broiler chickens using the trial protocol proposed by the World's Poultry Science Association. It was hypothesized that prececal P digestibility of SBM determined in the collaborating stations is similar. Three diets with different inclusion levels of SBM were mixed in a feed mill specialized in experimental diets and transported to 17 collaborating stations. Broiler chicks were raised on commercial starter diets according to station-specific management routine...
December 5, 2016: Poultry Science
https://www.readbyqxmd.com/read/27915271/changes-in-renal-gene-expression-associated-with-induced-ochratoxicosis-in-chickens-activation-and-deactivation-of-transcripts-after-varying-durations-of-exposure
#5
Cynthia P Zeferino, Kevin D Wells, Ana Silvia A M T Moura, George E Rottinghaus, David R Ledoux
Exposure to ochratoxin A (OTA) can lead to changes in global gene expression. This study investigated the individual expression of genes turned on and off in renal cells of chicks after different durations of exposure to dietary OTA. One hundred and eighty day-old male broiler chicks (Ross 308) were randomly assigned to a 3 × 3 factorial arrangement of treatments (3 levels of OTA: 0, 1 and 2 mg OTA/kg diet and 3 time periods: 7, 14 and 21 d). Birds were allocated to 36 pens (4 replicate pens of 5 birds each per treatment)...
December 3, 2016: Poultry Science
https://www.readbyqxmd.com/read/27909608/comparison-of-two-different-enteral-nutrition-protocol-in-critically-ill-patients
#6
Sibel Büyükçoban, Mert Akan, Uğur Koca, Merih Yıldız Eğlen, Meltem Çiçeklioğlu, Ömür Mavioğlu
OBJECTIVE: In this study, two enteral nutrition protocols with different gastric residual volumes (GRVs) and different monitoring intervals were compared with respect to gastrointestinal intolerance findings in intensive care unit (ICU) patients. METHODS: The study was carried out prospectively in 60 patients in the anaesthesiology and reanimation ICU under mechanical ventilation support, who were scheduled to take enteral feeding. Patients were sequentially divided into two groups: Group 1, GRV threshold of 100 mL, and monitoring interval of 4 hours, and Group 2, GRV threshold of 200 mL, monitoring interval of 8 hours...
October 2016: Turkish Journal of Anaesthesiology and Reanimation
https://www.readbyqxmd.com/read/27907920/aneuploidy-detection-and-mtdna-quantification-in-bovine-embryos-with-different-cleavage-onset-using-a-next-generation-sequencing-based-protocol
#7
Miroslav Hornak, David Kubicek, Petr Broz, Pavlina Hulinska, Katerina Hanzalova, Darren Griffin, Marie Machatkova, Jiri Rubes
Bovine embryos are now routinely used in agricultural systems as a means of disseminating superior genetics worldwide, ultimately with the aim of feeding an ever-growing population. Further investigations, common for human IVF embryos, thus have priority to improve cattle IVF, as has screening for aneuploidy (abnormal chromosome number). Although the incidence and consequences of aneuploidy are well documented in human preimplantation embryos, they are less well known for the embryos of other animals. To address this, we assessed aneuploidy levels in thirty-one 2-cell bovine embryos derived from early- and late-cleaving zygotes...
December 2, 2016: Cytogenetic and Genome Research
https://www.readbyqxmd.com/read/27903833/meal-distribution-of-dietary-protein-and-leucine-influences-long-term-muscle-mass-and-body-composition-in-adult-rats
#8
Layne E Norton, Gabriel J Wilson, Christopher J Moulton, Donald K Layman
BACKGROUND: Protein quantity and quality at a meal affect muscle protein synthesis (MPS); however, long-term effects of protein distribution at individual meals on adult muscle mass remain unknown. OBJECTIVE: We used a precise feeding protocol in adult rats to determine if optimizing postmeal MPS response by modifying the meal distribution of protein, and the amino acid leucine (Leu), would affect muscle mass. METHODS: Two studies were conducted with the use of male Sprague-Dawley rats (∼300 g) trained to consume 3 meals/d, then assigned to diet treatments with identical macronutrient contents (16% of energy from protein, 54% from carbohydrates, and 30% from fat) but differing in protein quality or meal distribution...
November 30, 2016: Journal of Nutrition
https://www.readbyqxmd.com/read/27903201/nutrition-in-the-intensive-care-unit-you-must-breathe-what-you-eat
#9
John F Cade, Daryl A Jones, Rinaldo Bellomo
The imprecision in prescribing of enteral nutrition in critically ill patients must result in occasions of overfeeding as well as underfeeding. Overfeeding could cause increased CO2 production and thus increased work of breathing and prolonged ventilator dependence. This possibility is supported by the limited relevant literature. We examined this possibility mathematically using the data in The Augmented versus Routine Approach to Giving Energy Trial (TARGET) feasibility study and in its main study protocol...
December 2016: Critical Care and Resuscitation: Journal of the Australasian Academy of Critical Care Medicine
https://www.readbyqxmd.com/read/27902988/smell-and-taste-to-improve-nutrition-in-very-preterm-infants-a-randomized-controlled-pilot-trial
#10
Friederike Beker, Gillian Opie, Elizabeth Noble, Yannan Jiang, Frank H Bloomfield
BACKGROUND: The perception of smell and taste, though present early in development, is not routinely considered in the care of preterm infants. Smell and taste are known to increase gut motility, insulin secretion, and the release of appetite, digestive and metabolic hormones. OBJECTIVE: We aimed to investigate the effect of regular smell and taste on the time from birth to full enteral feeds, and the feasibility of the study protocol in very preterm infants. METHODS: In a randomized controlled trial, infants <29 weeks' postmenstrual age (PA) were assigned to receive either the smell and taste of milk before each feed or to have no exposure to the smell and taste of milk (control)...
December 1, 2016: Neonatology
https://www.readbyqxmd.com/read/27895049/daily-feeding-and-protein-metabolism-rhythms-in-senegalese-sole-post-larvae
#11
Carmen Navarro-Guillén, Manuel Yúfera, Sofia Engrola
Fish hatcheries must adapt larval feeding protocols to the feeding behavior and metabolism pattern to obtain a more efficient feeds utilization. Fish larvae exhibit daily ingesting rhythms rather than ingesting food continuously throughout the day. The aim of this study was to determine the daily patterns of feed intake, protein digestibility, protein retention and catabolism in Senegalese sole post-larvae (Solea senegalensis; 33 days post-hatching) using (14)C-labelled Artemia protein and incubation in metabolic chambers...
November 28, 2016: Biology Open
https://www.readbyqxmd.com/read/27881690/acceptability-of-outpatient-ready-to-use-food-based-protocols-in-hiv-infected-senegalese-children-and-adolescents-within-the-maggsen-cohort-study
#12
Cecile Cames, Marie Varloteaux, Ndeye Ngone Have, Alhadji Bassine Diom, Philippe Msellati, Ngagne Mbaye, Helene Mbodj, Haby Sy Signate, Aminata Diack
OBJECTIVES: To assess the acceptability of ready-to-use food (RUF)-based outpatient protocols in HIV-infected children and adolescents with severe acute malnutrition (SAM) and moderate acute malnutrition (MAM). METHODS: Plumpy Nut and Plumpy Sup were supplied every 2 weeks and prescribed by weight to SAM and MAM children, respectively. Forty-three children, 24 MAM and 19 SAM, were enrolled. Organoleptic appreciation, feeding modalities, and perceptions surrounding RUF were recorded at week 2...
November 22, 2016: Food and Nutrition Bulletin
https://www.readbyqxmd.com/read/27878781/successful-elimination-of-extended-spectrum-beta-lactamase-esbl-producing-nosocomial-bacteria-at-a-neonatal-intensive-care-unit
#13
Borbála Szél, Zsolt Reiger, Edit Urbán, Andrea Lázár, Krisztina Mader, Ivelina Damjanova, Kamilla Nagy, Gyula Tálosi
BACKGROUND: Extended-spectrum beta-lactamase (ESBL)-producing Gram-negative bacteria are highly dangerous to neonates. At our Neonatal Intensive Care Unit (NICU), the presence of these bacteria became so threatening in 2011 that immediate intervention was required. METHODS: This study was conducted during a nearly two-year period consisting of three phases: retrospective (9 months), educational (3 months) and prospective (9 months). Based on retrospective data analysis, a complex management plan was devised involving the introduction of the INSURE protocol, changes to the antibiotic regimen, microbiological screening at short intervals, progressive feeding, a safer bathing protocol, staff hand hygiene training and continuous monitoring of the number of newly infected and newly colonized patients...
November 23, 2016: World Journal of Pediatrics: WJP
https://www.readbyqxmd.com/read/27875285/what-is-best-achievable-practice-in-implementing-the-enhanced-protein-energy-provision-via-the-enteral-route-feeding-protocol-in-intensive-care-units-in-the-united-states-results-of-a-multicenter-quality-improvement-collaborative
#14
Daren K Heyland, Margot Lemieux, Lin Shu, Kristen Quisenberry, Andrew G Day
BACKGROUND: The purpose of this study was to determine what was "best achievable practice" with the implementation of a novel enteral feeding protocol (Enhanced Protein-Energy Provision via the Enteral Route Feeding Protocol [PEP uP protocol]). METHODS: This study was a multicenter quality improvement collaborative wherein we describe nutrition practices and outcomes within PEP uP sites. We report the minimum, average, and maximal site-level performance on aspects related to nutrition practices and outcomes...
October 10, 2016: JPEN. Journal of Parenteral and Enteral Nutrition
https://www.readbyqxmd.com/read/27875094/laparoscopic-radical-gastrectomy-for-resectable-advanced-gastric-cancer-within-enhanced-recovery-programs-a-prospective-randomized-controlled-trial
#15
Xia Mingjie, Zhang Luyao, Tang Ze, Zhao YinQuan, Wang Quan
BACKGROUND: Enhanced recovery programs have become an important focus of perioperative management. A few studies have demonstrated the efficacy of an enhanced recovery after surgery (ERAS, which includes optimized pain control, restricted I.V. fluids, early initiation of postoperative oral feeding, and enforced mobilization) protocol in patients undergoing radical gastrectomy. We investigated the feasibility and safety of laparoscopic radical gastrectomy within ERAS programs. METHODS: In this single-center prospective randomized controlled trial conducted between September 2013 and August 2014, 149 consecutive locally advanced gastric cancer patients (T2-4, any N, M0) diagnosed by the CT scanning were allocated to either ERAS group (N = 73) or conventional pathway group (N = 76)...
November 22, 2016: Journal of Laparoendoscopic & Advanced Surgical Techniques. Part A
https://www.readbyqxmd.com/read/27866661/a-critical-question-for-nec-researchers-can-we-create-a-consensus-definition-of-nec-that-facilitates-research-progress
#16
REVIEW
Phillip V Gordon, Jonathan R Swanson, Brianna C MacQueen, Robert D Christensen
In the last decades the reported incidence of preterm necrotizing enterocolitis (NEC) has been declining in large part due to implementing comprehensive NEC prevention initiatives, including breast milk feeding, standardized feeding protocols, transfusion guidelines, and antibiotic stewardship and improving the rigor with which non-NEC cases are excluded from NEC data. However, after more than 60 years of NEC research in animal models, the promise of a "magic bullet" to prevent NEC has yet to materialize. There are also serious issues involving clinical NEC research...
November 17, 2016: Seminars in Perinatology
https://www.readbyqxmd.com/read/27861463/promoting-healthy-eating-and-active-playtime-by-connecting-to-nature-families-with-preschool-children-evaluation-of-pilot-study-play-grow
#17
Tanja Sobko, Zhenzhen Jia, Matthew Kaplan, Alfred Lee, Chia-Huei Tseng
BackgroundThis pilot project aimed to evaluate the "Play&Grow" program which promotes age-appropriate dietary habits and playtime healthy routines through "connectedness to nature" experiences in Hong Kong families with young children.Method38 pre-schoolers (aged 33.97 ± 9.38 months), mothers and their domestic workers were recruited. The families attended one workshop/week for a 4-month period, consisting of: 1) health topic; 2) food games; 3) nature-related outdoor activities.ResultsFeeding practices, particularly Promoting and Encouragement to eat (PE) and Instrumental Feeding (IF) improved after the intervention (p =...
November 18, 2016: Pediatric Research
https://www.readbyqxmd.com/read/27860296/impact-of-subnormothermic-machine-perfusion-preservation-in-severely-steatotic-rat-livers-a-detailed-assessment-in-an-isolated-setting
#18
Y Okamura, K Hata, H Tanaka, H Hirao, T Kubota, O Inamoto, S Kageyama, I Tamaki, N Yermek, J Yoshikawa, S Uemoto
Current drastic shortage of donor organs has led to acceptance of extended-criteria donors for transplantation, despite higher risk of primary non-function. Here, we report the impact of subnormothermic machine perfusion (SMP) preservation on the protection of >50% macro-steatotic livers. Dietary hepatic steatosis was induced in Wistar rats via 2-day fasting and subsequent 3-day re-feeding with a fat-free, carbohydrate-rich diet. This protocol induces 50-60% macrovesicular steatosis, which should be discarded when preserved via cold storage (CS)...
November 10, 2016: American Journal of Transplantation
https://www.readbyqxmd.com/read/27859478/effect-of-a-new-treatment-protocol-called-functional-chewing-training-on-chewing-function-in-children-with-cerebral-palsy-a-double-blind-randomised-controlled-trial
#19
S Serel Arslan, N Demir, A A Karaduman
Cerebral palsy (CP) is a group of permanent sensorimotor impairments. Children with CP have various feeding difficulties including chewing disorder, which may affect their nutritional status. Functional Chewing Training (FuCT) was designed as a holistic approach to improve chewing function by providing postural alignment, sensory and motor training, and food and environmental adjustments. This study aimed to investigate the effect of FuCT on chewing function in children with CP. This study was designed as a double-blind, randomised controlled trial...
November 17, 2016: Journal of Oral Rehabilitation
https://www.readbyqxmd.com/read/27856406/efficacy-and-safety-of-high-dose-ivermectin-for-reducing-malaria-transmission-ivermal-protocol-for-a-double-blind-randomized-placebo-controlled-dose-finding-trial-in-western-kenya
#20
Menno R Smit, Eric Ochomo, Ghaith Aljayyoussi, Titus Kwambai, Bernard Abong'o, Nabie Bayoh, John Gimnig, Aaron Samuels, Meghna Desai, Penelope A Phillips-Howard, Simon Kariuki, Duolao Wang, Steve Ward, Feiko O Ter Kuile
BACKGROUND: Innovative approaches are needed to complement existing tools for malaria elimination. Ivermectin is a broad spectrum antiparasitic endectocide clinically used for onchocerciasis and lymphatic filariasis control at single doses of 150 to 200 mcg/kg. It also shortens the lifespan of mosquitoes that feed on individuals recently treated with ivermectin. However, the effect after a 150 to 200 mcg/kg oral dose is short-lived (6 to 11 days). Modeling suggests higher doses, which prolong the mosquitocidal effects, are needed to make a significant contribution to malaria elimination...
November 17, 2016: JMIR Research Protocols
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