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B Kieronczyk, M Sassek, E Pruszynska-Oszmalek, P Kolodziejski, M Rawski, S Swiatkiewicz, D Józefiak
The aim of this study was to investigate the effect of dietary supplementation with nisin alone or in combination with salinomycin or monensin on broiler chickens in terms of growth performance, selected blood parameters, digestive enzyme activity, apparent nutrient digestibility, and tibiotarsus mineralization, as well as selected gastrointestinal tract (GIT) organ weights, intestinal length, and central immune organ weights. Two independent experiments, each including 400 one-day-old female Ross 308 chicks differing in ionophore coccidiostats, i...
September 1, 2017: Poultry Science
Xuan Tang, Shuangshuang Wu, Xiaofeng Wang, Qing Gu, Ping Li
Strains belonging to the genus of Staphylococci, such as Staphylococcus aureus are common pathologic bacteria which may cause nosocomial cross infection and food contamination. Plantaricin EF (PlnEF), a two-peptide nonlantibiotic bacteriocin produced by Lactobacillus plantarum strains, shows great inhibitory effects against Gram-positive Staphylococci strains. To overproduce this two-peptide bacteriocin, plnE and plnF genes were cloned into pET32a (+) vector and heterologously expressed in Escherichia coli by fusion with His6-tag in this study...
October 13, 2017: Protein Expression and Purification
Juan Borrero, Eoin Kelly, Paula M O'Connor, Philip Kelleher, Colm Scully, Paul D Cotter, Jennifer Mahony, Douwe van Sinderen
Bacteriocins from lactic acid bacteria (LAB) are of increasing interest in recent years due to their potential as natural preservatives against food and beverage spoilage microorganisms. In a screening study for LAB, we isolated a strain, Lactobacillus plantarum NI326, from olives with activity against a strain belonging to the beverage-spoilage bacterium Alicyclobacillus acidoterrestris spp. Genome sequencing of the strain enabled the identification of a gene cluster encoding a putative circular bacteriocin and proteins involved in its modification, transport and immunity...
October 13, 2017: Applied and Environmental Microbiology
Hugh M B Harris, Maxence J B Bourin, Marcus J Claesson, Paul W O'Toole
The genus Lactobacillus is a diverse group with a combined species count of over 200. They are the largest group within the lactic acid bacteria and one of the most important bacterial groups involved in food microbiology and human nutrition because of their fermentative and probiotic properties. Lactobacillus salivarius, a species commonly isolated from the gastrointestinal tract of humans and animals, has been described as having potential probiotic properties and results of previous studies have revealed considerable functional diversity existing on both the chromosomes and plasmids...
August 2017: Microbial Genomics
Uraporn Phumisantiphong, Kanokrat Siripanichgon, Onrapak Reamtong, Pornphan Diraphat
The emergence of multidrug-resistant enterococci (MDRE) and particularly vancomycin-resistant enterococci (VRE) is considered a serious health problem worldwide, causing the need for new antimicrobials. The aim of this study was to discover and characterize bacteriocin against clinical isolates of MDRE and VRE. Over 10,000 bacterial isolates from water, environment and clinical samples were screened. E. faecalis strain 478 isolated from human feces produced the highest antibacterial activity against several MDRE and VRE strains...
2017: PloS One
Lei Liu, Ruiyun Wu, Jinlan Zhang, Nan Shang, Pinglan Li
Biofilms help bacteria survive under adverse conditions, and the quorum sensing (QS) system plays an important role in regulating their activities. Quorum sensing inhibitors (QSIs) have great potential to inhibit pathogenic biofilm formation and are considered possible replacements for antibiotics; however, further investigation is required to understand the mechanisms of action of QSIs and to avoid inhibitory effects on beneficial bacteria. Lactobacillus paraplantarum L-ZS9, isolated from fermented sausage, is a bacteriocin-producing bacteria that shows potential to be a probiotic starter...
2017: Frontiers in Microbiology
E E Escamilla-Martínez, Y M Álvarez Cisneros, F J Fernández, M Quirasco-Baruch, E Ponce-Alquicira
The Enterococcus faecium strain MXVK29, isolated from fermented sausages, produces a bacteriocin with a molecular mass of 3.5 kDa that belongs to the class of enterocins II.1, according to the terminal amino acid sequence, and has been identified as enterocin A. This bacteriocin is active against selected strains of Listeria, Staphylococcus, Pediococcus, and Enterococcus. In this study, we identified the genes adjacent to the structural gene for this bacteriocin, such as the immunity gene (entI) and the inducer gene (entF)...
October 9, 2017: Journal of Food Protection
Melinda A Engevik, James Versalovic
Commensal and beneficial microbes secrete myriad products which target the mammalian host and other microbes. These secreted substances aid in bacterial niche development, and select compounds beneficially modulate the host and promote health. Microbes produce unique compounds which can serve as signaling factors to the host, such as biogenic amine neuromodulators, or quorum-sensing molecules to facilitate inter-bacterial communication. Bacterial metabolites can also participate in functional enhancement of host metabolic capabilities, immunoregulation, and improvement of intestinal barrier function...
October 2017: Microbiology Spectrum
Natalia S Ríos Colombo, Miriam C Chalón, Silvia A Navarro, Augusto Bellomio
This review attempts to analyze the mechanism of action and immunity of class IIa bacteriocins. These peptides are promising alternative food preservatives and they have a great potential application in medical sciences. Class IIa bacteriocins act on the cytoplasmic membrane of Gram-positive cells dissipating the transmembrane electrical potential by forming pores. However, their toxicity and immunity mechanism remains elusive. Here we discuss the role of the mannose phosphotransferase system (man-PTS) as the receptor for class IIa bacteriocins and the influence of the membrane composition on the activity of these antimicrobial peptides...
October 5, 2017: Current Genetics
Jeella Z Acedo, Cherry Ibarra Romero, Sarah T Miyata, Alysson H Blaine, Lynn M McMullen, John C Vederas, Marco J van Belkum
Enterococcus canintestini 49, isolated from dog feces, is active against Clostridium perfringens, vancomycin-resistant enterococci, and Listeria monocytogenes Its draft genome sequence reported herein contains a gene cluster encoding multiple bacteriocins and indicates the absence of genes for virulence factors. These characteristics signify the strain's potential for use as a probiotic.
October 5, 2017: Genome Announcements
Lyudmila Boyanova, Galina Gergova, Rumyana Markovska, Daniel Yordanov, Ivan Mitov
The aim of the study was to detect anti-Helicobacter pylori activity of seven Lactobacillus delbrueckii subsp. bulgaricus (GLB) strains by four cell-free supernatant (CFS) types. Activity of non-neutralized and non-heat-treated (CFSs1), non-neutralized and heat-treated (CFSs2), pH neutralized, catalase-treated and non-heat-treated (CFSs3), or neutralized, catalase- and heat-treated (CFSs4) CFSs against 18 H. pylori strains (11 of which with antibiotic resistance) was evaluated. All GLB strains produced bacteriocin-like inhibitory substances (BLISs), the neutralized CFSs of two GLB strains inhibited >81% of test strains and those of four GLB strains were active against >71% of antibiotic resistant strains...
October 4, 2017: Letters in Applied Microbiology
Šarūnas Paškevičius, Urtė Starkevič, Audrius Misiūnas, Astra Vitkauskienė, Yuri Gleba, Aušra Ražanskienė
The emergence, persistence and spread of antibiotic-resistant human pathogenic bacteria heralds a growing global health crisis. Drug-resistant strains of gram-negative bacteria, such as Pseudomonas aeruginosa, are especially dangerous and the medical and economic burden they impose underscore the critical need for finding new antimicrobials. Recent studies have demonstrated that plant-expressed bacteriocins of the colicins family can be efficient antibacterials against all major enteropathogenic strains of E...
2017: PloS One
Dean Scholl
Many dsDNA bacterial viruses (bacteriophages/phages) have long tail structures that serve as organelles for DNA delivery to host targets. These structures, particularly those of Myoviridae and Siphoviridae phages, have an evolutionary relationship with other cellular biological entities that share the common function of penetrating the bacterial envelope. Among these are type VI secretion systems, insecticidal protein complexes, and bacteriocins. Phage tail-like bacteriocins (PTLBs) are widespread in bacteria, comprising different types that likely evolved independently...
September 29, 2017: Annual Review of Virology
Meng Gao, Jingjing Zhou, Zhengding Su, Yongqi Huang
Azurin secreted by Pseudomonas aeruginosa is an anticancer bacteriocin, which preferentially enters human cancer cells and induces apoptosis or growth inhibition. It turns out that azurin is a multi-target anticancer agent interfering in the p53 signaling pathway and the non-receptor tyrosine kinases signaling pathway. This suggests that azurin exerts its anticancer activity by interacting with multiple targets and interfering in multiple steps in disease progression. Therefore, azurin could overcome resistance to therapy...
September 27, 2017: Protein Science: a Publication of the Protein Society
Özgün C O Umu, Knut Rudi, Dzung B Diep
The gut microbiota is considered an organ that co-develops with the host throughout its life. The composition and metabolic activities of the gut microbiota are subject to a complex interplay between the host genetics and environmental factors, such as lifestyle, diet, stress and antimicrobials. It is evident that certain prebiotics, and antimicrobials produced by lactic acid bacteria (LAB), can shape the composition of the gut microbiota and its metabolic activities to promote host health and/or prevent diseases...
2017: Microbial Ecology in Health and Disease
Jamshid Razmyar, Seyed Mostafa Peighambari, Amir Hossein Zamani
Necrotic enteritis due to Clostridium perfringens strains harboring the netB gene is a well-known disorder in poultry. The aim of this study was to investigate the association of a novel bacteriocin, perfrin, with netB among isolates from healthy and diseased ostriches and broiler chickens. Forty-six C. perfringens isolates from broiler chickens and ostriches collected from 2010 to 2014 were included in this study and subjected to PCR to detect netB and perfrin genes. Six (60%) and 9 (25%) isolates were positive for both netB and perfrin genes in broilers and ostriches, respectively...
September 2017: Avian Diseases
Zhoujie Xie, Zhao Zhang, Liwei Liu, Xiaochen Liu, Yihua Chen
Dental biofilms are composed of hundreds of bacterial species, among which Streptococcus mutans is widely recognized as the major pathogen of dental caries. The cariogenic potential of S. mutans is related to its ability to form a robust biofilm on the tooth surface and its acidogenic and acid-tolerant properties. Co-evolution of S. mutans with the host has resulted in the diversity of secondary metabolism of S. mutans in strain level. A variety of secondary metabolites, including 10 bacteriocins (mutacins) and one hybrid Polyketide/Non-Ribosomal Peptide type compound, have been characterized...
September 25, 2017: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
Gabriel Quintana, Maria V Niederle, Carlos J Minahk, Gianluca Picariello, María E F Nader-Macías, Sergio E Pasteris
Lactococcus lactis subsp. lactis CRL 1584 isolated from a bullfrog hatchery produces a bacteriocin that inhibits both indigenous Citrobacter freundii (a Red-Leg Syndrome related pathogen) and Lactobacillus plantarum, and Listeria monocytogenes as well. Considering that probiotics requires high cell densities and/or bacteriocin concentrations, the effect of the temperature on L. lactis growth and bacteriocin production was evaluated to find the optimal conditions. Thus, the growth rate was maximal at 36 °C, whereas the highest biomass and bacteriocin activity was achieved between 20 and 30 °C and 20-25 °C, respectively...
September 27, 2017: World Journal of Microbiology & Biotechnology
Qianwen Xi, Jin Wang, Renpeng Du, Fangkun Zhao, Ye Han, Zhijiang Zhou
The aims of this study were to purify and characterize a bacteriocin produced by a strain of Enterococcus faecalis TG2 and to test the safety of the strain. In this work, the active peptide was purified through precipitation with 70% saturated ammonium sulfate, cation-exchange chromatography, and gel filtration. The specific activity of purified bacteriocin was 30,073.42 AU/mg of protein, which corresponded to a 33.34-fold increase. The molecular mass of the purified bacteriocin was 6.3362 kDa determined by LC-MS/MS...
September 27, 2017: Applied Biochemistry and Biotechnology
Ralf Lopes, Louise Cerdeira, Grace S Tavares, Jeronimo C Ruiz, Jochen Blom, Elvira C A Horácio, Hilário C Mantovani, Marisa Vieira de Queiroz
Diseases caused by phytopathogenic microorganisms account for enormous losses for agribusiness. Although Bacillus species are recognized as being antimicrobial producers and some may provide benefits to plants, the association between Bacillus toyonensis and plants has not been studied. In this study, the whole-genome sequenced endophytic B. toyonensis BAC3151, which has demonstrated antimicrobial activity and quorum sensing inhibition of phytopathogenic bacteria, was investigated for its potential for the production of compounds for biocontrol of plant pathogens...
September 25, 2017: World Journal of Microbiology & Biotechnology
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