keyword
MENU ▼
Read by QxMD icon Read
search

Perivascular macrophage

keyword
https://www.readbyqxmd.com/read/28194443/sustained-inflammation-after-pericyte-depletion-induces-irreversible-blood-retina-barrier-breakdown
#1
Shuntaro Ogura, Kaori Kurata, Yuki Hattori, Hiroshi Takase, Toshina Ishiguro-Oonuma, Yoonha Hwang, Soyeon Ahn, Inwon Park, Wataru Ikeda, Sentaro Kusuhara, Yoko Fukushima, Hiromi Nara, Hideto Sakai, Takashi Fujiwara, Jun Matsushita, Masatsugu Ema, Masanori Hirashima, Takashi Minami, Masabumi Shibuya, Nobuyuki Takakura, Pilhan Kim, Takaki Miyata, Yuichiro Ogura, Akiyoshi Uemura
In the central nervous system, endothelial cells (ECs) and pericytes (PCs) of blood vessel walls cooperatively form a physical and chemical barrier to maintain neural homeostasis. However, in diabetic retinopathy (DR), the loss of PCs from vessel walls is assumed to cause breakdown of the blood-retina barrier (BRB) and subsequent vision-threatening vascular dysfunctions. Nonetheless, the lack of adequate DR animal models has precluded disease understanding and drug discovery. Here, by using an anti-PDGFRβ antibody, we show that transient inhibition of the PC recruitment to developing retinal vessels sustained EC-PC dissociations and BRB breakdown in adult mouse retinas, reproducing characteristic features of DR such as hyperpermeability, hypoperfusion, and neoangiogenesis...
February 9, 2017: JCI Insight
https://www.readbyqxmd.com/read/28193684/an-agonist-of-the-protective-factor-sirt1-improves-functional-recovery-and-promotes-neuronal-survival-by-attenuating-inflammation-after-spinal-cord-injury
#2
Haihong Chen, Hao Ji, Ming Zhang, Zude Liu, Lifeng Lao, Chao Deng, Jianwei Chen, Guibin Zhong
Targeting posttraumatic inflammation is crucial for improving locomotor function. SIRT1 has been shown to play a critical role in disease processes such as hepatic inflammation, rheumatoid arthritis and acute lung inflammation by regulating inflammation. However, the role of SIRT1 in spinal cord injury (SCI) is unknown. We hypothesized that SIRT1 plays an important role in improving locomotor function after SCI by regulating neuroinflammation. In this study, we investigated the effect of SIRT1 in SCI using pharmacological intervention (SRT1720) and the Mx1-Cre/loxP recombination system to knock out target genes...
February 13, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/28179107/pro-inflammatory-immune-to-brain-signaling-is-involved-in-neuroendocrine-responses-to-acute-emotional-stress
#3
Jordi Serrats, Jan-Sebastian Grigoleit, Elena Alvarez-Salas, Paul E Sawchenko
Activation of the hypothalamo-pituitary-adrenal (HPA) axis by inflammatory stressors (e.g., bacterial lipopolysaccharide) is thought to involve vascular transduction of circulating cytokines, with perivascular macrophages (PVMs) along with endothelia, effecting activation of HPA control circuitry via inducible (cyclooxygenase-2- or COX-2- dependent) prostaglandin synthesis. To test the stressor-specificity of this mechanism, we examined whether ablation of PVMs or pharmacologic blockade of COX activity affected HPA responses to a representative emotional stressor, restraint...
February 4, 2017: Brain, Behavior, and Immunity
https://www.readbyqxmd.com/read/28148272/dose-dependent-induction-of-astrocyte-activation-and-reactive-astrogliosis-in-mouse-brain-following-maternal-exposure-to-carbon-black-nanoparticle
#4
Atsuto Onoda, Ken Takeda, Masakazu Umezawa
BACKGROUND: Recent studies indicate that maternal exposure to ambient ultrafine particles and nanoparticles has adverse effects of on the central nervous system. Quantitative dose-response data is required to better understand the developmental neurotoxicity of nanoparticles. The present study investigated dose-dependent effects of maternal exposure to carbon black nanoparticle (CB-NP) on astrocyte in the brains of mouse offspring. METHODS: A CB-NP suspension (2...
2, 2017: Particle and Fibre Toxicology
https://www.readbyqxmd.com/read/28125278/roles-for-the-cx3cl1-cx3cr1-and-ccl2-ccr2-chemokine-systems-in-hypoxic-pulmonary-hypertension
#5
Valérie Amsellem, Shariq Abid, Lucie Poupel, Aurélien Parpaleix, Mathieu Rodero, Guillaume Gary-Bobo, Mehdi Latiri, Jean-Luc Dubois-Rande, Larissa Lipskaia, Christophe Combadiere, Serge Adnot
Monocytes/macrophages are major effectors of lung inflammation associated with various forms of pulmonary hypertension (PH). Interactions between the CCL2/CCR2 and CX3CL1/CX3CR1 chemokine systems that guide phagocyte infiltration are incompletely understood. Our objective was to explore the individual and combined actions of CCL2/CCR2 and CX3CL1/CX3CR1 in hypoxia-induced PH in mice, particularly their roles in monocyte trafficking, macrophage polarization, and pulmonary vascular remodeling. The development of hypoxia-induced PH was associated with marked increases in lung levels of CX3CR1, CCR2, and their respective ligands CX3CL1 and CCL2...
January 26, 2017: American Journal of Respiratory Cell and Molecular Biology
https://www.readbyqxmd.com/read/28121875/advanced-imaging-techniques-for-investigation-of-acellular-dermal-matrix-biointegration
#6
Brent R DeGeorge, Bo Ning, Lisa S Salopek, Angela Pineros-Fernandez, George T Rodeheaver, Shayn Peirce-Cottler, Song Hu, Patrick S Cottler, Christopher A Campbell
BACKGROUND: Biointegration, a concept involving a dynamic interplay among three processes-inflammation, cellular infiltration, and angiogenesis-is key to understanding the interaction between acellular dermal matrices and the host. The current standard for evaluating acellular dermal matrix biointegration involves histologic analysis at fixed time points; however, the authors' approach uses advanced imaging techniques to serially assess biointegration in real time. METHODS: The authors have adapted two advanced imaging techniques-two-photon microscopy and photoacoustic microscopy-to investigate biointegration in a murine deepithelialized dorsal skin-fold window chamber model, specifically engineered to recapitulate the host microenvironment of acellular dermal matrix-assisted breast reconstruction...
February 2017: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/28116726/isolation-and-differentiation-of-human-macrophages
#7
Francisco J Rios, Rhian M Touyz, Augusto C Montezano
Macrophage subtypes display protective or pathogenic activities in vascular lesions. They recognize and engulf modified lipids, accumulate in the arterial intima, contributing to the atherosclerosis development. In the heart, vascular tissues and perivascular adipose tissues, there is increasing evidence that macrophages play a role in endothelial dysfunction, vascular inflammation and remodeling. In this chapter we describe protocols to isolate human monocytes from peripheral blood mononuclear cell and how to differentiate them into macrophages by using growth factors (granulocyte-macrophage colony-stimulating factor-GM-CSF or colony-stimulating factor-M-CSF) or by different cell culture medium concentration...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28093272/a-method-for-obtaining-simian-immunodeficiency-virus-rna-sequences-from-laser-capture-microdissected-and-immune-captured-cd68-and-cd163-macrophages-from-frozen-tissue-sections-of-bone-marrow-and-brain
#8
Jaclyn Mallard, Emily Papazian, Caroline Soulas, David J Nolan, Marco Salemi, Kenneth C Williams
Laser capture microdissection (LCM) is used to extract cells or tissue regions for analysis of RNA, DNA or protein. Several methods of LCM are established for different applications, but a protocol for consistently obtaining lentiviral RNA from LCM captured immune cell populations is not described. Obtaining optimal viral RNA for analysis of viral genes from immune-captured cells using immunohistochemistry (IHC) and LCM is challenging. IHC protocols have long antibody incubation times that increase risk of RNA degradation...
March 2017: Journal of Immunological Methods
https://www.readbyqxmd.com/read/28087417/culture-media-based-selection-of-endothelial-cells-pericytes-and-perivascular-resident-macrophage-like-melanocytes-from-the-young-mouse-vestibular-system
#9
Jinhui Zhang, Songlin Chen, Jing Cai, Zhiqiang Hou, Xiaohan Wang, Allan Kachelmeier, Xiaorui Shi
The vestibular blood-labyrinth barrier (BLB) is comprised of perivascular-resident macrophage-like melanocytes (PVM/Ms) and pericytes (PCs), in addition to endothelial cells (ECs) and basement membrane (BM), and bears strong resemblance to the cochlear BLB in the stria vascularis. Over the past few decades, in vitro cell-based models have been widely used in blood-brain barrier (BBB) and blood-retina barrier (BRB) research, and have proved to be powerful tools for studying cell-cell interactions in their respective organs...
January 11, 2017: Hearing Research
https://www.readbyqxmd.com/read/28063251/perivascular-adipose-tissue-inflammation-in-vascular-disease
#10
REVIEW
Ryszard Nosalski, Tomasz J Guzik
Perivascular adipose tissue (PVAT) plays a critical role in the pathogenesis of cardiovascular disease. In vascular pathologies perivascular adipose tissue increases in volume and becomes dysfunctional, with altered cellular composition and molecular characteristics. PVAT dysfunction is characterized by its inflammatory character, oxidative stress, diminished production of vaso-protective adipocyte derived relaxing factors (ADRFs) and increased production of paracrine factors such as resistin, leptin, cytokines (IL-6, TNF-α) and chemokines (RANTES, MCP-1)...
January 7, 2017: British Journal of Pharmacology
https://www.readbyqxmd.com/read/28057474/hiv-1-tat-disrupts-blood-brain-barrier-integrity-and-increases-phagocytic-perivascular-macrophages-and-microglia-in-the-dorsal-striatum-of-transgenic-mice
#11
Crystal R Leibrand, Jason J Paris, M Said Ghandour, Pamela E Knapp, Woong-Ki Kim, Kurt F Hauser, MaryPeace McRae
HIV-1 infection results in blood-brain barrier (BBB) disruption, which acts as a rate-limiting step for HIV-1 entry into the CNS and for subsequent neuroinflammatory/neurotoxic actions. One mechanism by which HIV may destabilize the BBB involves actions of the HIV-1 regulatory protein, trans-activator of transcription (Tat). We utilized a conditional, Tat-expressing transgenic murine model to examine the influence of Tat1-86 expression on BBB integrity and to assess the relative numbers of phagocytic perivascular macrophages and microglia within the CNS in vivo...
February 15, 2017: Neuroscience Letters
https://www.readbyqxmd.com/read/28052367/morphoea-with-prominent-plasma-cell-endoneuritis
#12
M Singh, N Farquharson, C Owen, A J Howat, S Singh, N Francis, E Calonje
Morphoea (localized scleroderma) is a cutaneous inflammatory condition characterized by the development of indurated and discoloured plaques. The histological features of morphoea typically include a superficial and deep perivascular and periadnexal chronic inflammatory infiltrate associated with variable degrees of dermal and/or subcutaneous sclerosis. The infiltrate is typically composed of lymphocytes, macrophages and conspicuous plasma cells. The early stages of morphoea may have a very prominent inflammatory infiltrate associated with subtle sclerosis...
January 4, 2017: Clinical and Experimental Dermatology
https://www.readbyqxmd.com/read/28049898/developmental-toxicity-of-nanoparticles-on-the-brain
#13
Masakazu Umezawa, Atsuto Onoda, Ken Takeda
 The toxicity of nanoparticles (nanotoxicology) is being investigated to understand both the health impacts of atmospheric ultrafine particles-the size of which is a fraction (<0.1 μm aerodynamic diameter) of that of PM2.5 (<2.5 μm diameter)-and the safer use of engineered nanomaterials. Developmental toxicity of nanoparticles has been studied since their transfer from pregnant body to fetal circulation and offspring body was first reported. Here we reviewed the developmental toxicity of nanoparticles on the brain, one of the most important organs in maintenance of mental health and high quality of life...
2017: Yakugaku Zasshi: Journal of the Pharmaceutical Society of Japan
https://www.readbyqxmd.com/read/28012941/age-related-arterial-immune-cell-infiltration-in-mice-is-attenuated-by-caloric-restriction-or-voluntary-exercise
#14
Daniel W Trott, Grant D Henson, Mi H T Ho, Sheilah A Allison, Lisa A Lesniewski, Anthony J Donato
Age-related arterial inflammation is associated with dysfunction of the arteries and increased risk for cardiovascular disease. To determine if aging increases arterial immune cell infiltration as well as the populations of immune cells principally involved, we tested the hypothesis that large elastic and resistance arteries in old mice would exhibit increased immune cell infiltration compared to young controls. Additionally, we hypothesized that vasoprotective lifestyle interventions such as lifelong caloric restriction or 8weeks of voluntary wheel running would attenuate age-related arterial immune cell infiltration...
December 22, 2016: Experimental Gerontology
https://www.readbyqxmd.com/read/28011042/glial-changes-and-evidence-for-apoptosis-in-the-brain-of-cats-infected-by-cytauxzoon-felis
#15
L L Clarke, P M Krimer, D R Rissi
Ischaemic neuropathological changes associated with Cytauxzoon felis infection in cats have been reported recently. This paper describes the associated glial changes and the evidence for apoptosis in the brain of cats infected naturally by C. felis. Sections of brain from eight affected cats and eight age- and sex-matched control cats were evaluated by immunohistochemistry for expression of glial fibrillary acidic protein, CD18 and cleaved caspase-3. Vascular changes in the leptomeninges and parenchyma, the number of positive astrocytes and phagocytic cells (microglia or macrophages) and the average astrocytic cytoplasmic area and number and length of astrocytic processes were quantified, and a mean value for the grey and white matter in both groups was generated...
December 20, 2016: Journal of Comparative Pathology
https://www.readbyqxmd.com/read/28005953/pathophysiological-consequences-of-a-break-in-s1p1-dependent-homeostasis-of-vascular-permeability-revealed-by-s1p1-competitive-antagonism
#16
Marc Bigaud, Zuhal Dincer, Birgit Bollbuck, Janet Dawson, Nicolau Beckmann, Christian Beerli, Gina Fishli-Cavelti, Michaela Nahler, Daniela Angst, Philipp Janser, Heike Otto, Elisabeth Rosner, Rene Hersperger, Christian Bruns, Jean Quancard
RATIONAL: Homeostasis of vascular barriers depends upon sphingosine 1-phosphate (S1P) signaling via the S1P1 receptor. Accordingly, S1P1 competitive antagonism is known to reduce vascular barrier integrity with still unclear pathophysiological consequences. This was explored in the present study using NIBR-0213, a potent and selective S1P1 competitive antagonist. RESULTS: NIBR-0213 was tolerated at the efficacious oral dose of 30 mg/kg BID in the rat adjuvant-induced arthritis (AiA) model, with no sign of labored breathing...
2016: PloS One
https://www.readbyqxmd.com/read/28004071/-n-acetyl-l-cysteine-reduces-the-ozone-induced-lung-inflammation-response-in-mice
#17
Qing-Zi Chen, Zhao-Di Fu, Yu-Bo Zhou, Li-Fen Zhou, Chun-Tao Yang, Jian-Hua Li
In this study, we investigated the protective effect of the antioxidant N-acetyl-L-cysteine (NAC) on the lung inflammation caused by ozone (O3) exposure in mice. Thirty-two C57BL/6 mice were randomly divided into control group, O3 group, O3+NAC group and NAC group. Mice were exposed to O3 (1.0 ppm) or fresh air for 3 h on the day 1, day 3 and day 5, respectively. NAC (100 mg/kg) was intraperitoneally applied to the mice 1 h before each exposure. At 24 h after the 3-time exposure, the alveolar wall structure was severely damaged and the infiltrated inflammatory cells were apparent perivascularly and peribronchiolarly...
December 25, 2016: Sheng Li Xue Bao: [Acta Physiologica Sinica]
https://www.readbyqxmd.com/read/27974594/complement-mediated-inhibition-of-adiponectin-regulates-perivascular-inflammation-and-vascular-injury-in-hypertension
#18
Cheng-Chao Ruan, Yu Ma, Qian Ge, Yan Li, Li-Min Zhu, Ying Zhang, Ling-Ran Kong, Qi-Hong Wu, Fanghong Li, Lixian Cheng, Allan Z Zhao, Ding-Liang Zhu, Ping-Jin Gao
Perivascular adipose tissue (PVAT)-derived adiponectin (APN) is a secreted adipokine that protects against hypertension-related cardiovascular injury. However, the regulation of APN expression in hypertension remains to be explored. In this study, we demonstrated that down-regulation of APN was associated with complement activation in the PVAT of desoxycorticosterone acetate (DOCA)-salt hypertensive mice. Complement 3-deficient hypertensive mice were protected from ANP decrease in the PVAT. APN deficiency blockaded the protective effects of complement inhibition against hypertensive vascular injury...
December 14, 2016: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/27943116/cellular-players-of-hematopoietic-stem-cell-mobilization-in-the-bone-marrow-niche
#19
REVIEW
Joshua Tay, Jean-Pierre Levesque, Ingrid G Winkler
Hematopoietic stem cells (HSC) reside in perivascular regions of the bone marrow (BM) embedded within a complex regulatory unit called the niche. Cellular components of HSC niches include vascular endothelial cells, mesenchymal stromal progenitor cells and a variety of mature hematopoietic cells such as macrophages, neutrophils, and megakaryocytes-further regulated by sympathetic nerves and complement components as described in this review. Three decades ago the discovery that cytokines induce a large number of HSC to mobilize from the BM into the blood where they are easily harvested, revolutionised the field of HSC transplantation-curative for immune-deficiencies and some malignancies...
December 10, 2016: International Journal of Hematology
https://www.readbyqxmd.com/read/27939504/anti-inflammatory-effects-on-ischemia-reperfusion-injured-lung-transplants-by-the-cluster-of-differentiation-26-dipeptidylpeptidase-4-cd26-dpp4-inhibitor-vildagliptin
#20
Jae-Hwi Jang, Yoshito Yamada, Florian Janker, Ingrid De Meester, Lesley Baerts, Gwendolyn Vliegen, Ilhan Inci, Shampa Chatterjee, Walter Weder, Wolfgang Jungraithmayr
OBJECTIVES: We showed previously that stromal cell-derived factor 1 (SDF-1) is a substrate of cluster of differentiation 26/dipeptidylpeptidase 4 (CD26/DPP4) and exerts regenerative properties on acute lung ischemia-reperfusion injury on CD26/DPP4 inhibition. Here, we extend our studies to test whether an intermediate recovery of lung transplants from ischemia/reperfusion injury by CD26/DPP4 inhibition can be achieved for up to 14 days. METHODS: Syngeneic mouse lung transplantation (Tx) was performed in C57BL/6 and in CD26-/- mice by applying 18 hours of cold ischemia...
November 15, 2016: Journal of Thoracic and Cardiovascular Surgery
keyword
keyword
1395
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"