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https://www.readbyqxmd.com/read/28092911/behaviour-of-cd11b-positive-cells-in-an-animal-model-of-laser-induced-choroidal-neovascularisation
#1
Lu Li, Peter Heiduschka, Anne F Alex, Daniel Niekämper, Nicole Eter
BACKGROUND/AIM: Immune cells, e.g. microglial cells of the retina, appear to be involved in pathological processes in neovascular age-related macular degeneration. Therefore, the purpose of this study was to immunohistochemically check the expression of various factors and cytokines by CD11b-positive (CD11b+) immune cells in an animal model of choroidal neovascularisation (CNV). METHODS: We used the animal model of laser-induced CNV in mice. Eyes were isolated at 1, 4, 7, and 14 days after laser treatment...
January 17, 2017: Ophthalmologica. Journal International D'ophtalmologie
https://www.readbyqxmd.com/read/28092794/%C3%AE-defensin-3-modulates-macrophage-activation-and-orientation-during-acute-inflammatory-response-to-porphyromonas-gingivalis-lipopolysaccharide
#2
Jinglu Lyu, Tianying Bian, Bin Chen, Di Cui, Lili Li, Ling Gong, Fuhua Yan
β-defensin 3, a multifunctional antimicrobial peptide, has immuno-regulatory activities. We investigated the modulatory mechanism of human β-defensin 3 (hBD3) on acute inflammatory response resulted from Porphyromonas gingivalis lipopolysaccharide (P.g-LPS), which plays a pro-inflammatory role in periodontal infection and its derived systemic inflammation. P.g-LPS was administrated to mice and murine macrophages alone or along with hBD3. P.g-LPS could lead to acute inflammation as soon as 2h. And it was observed that hBD3 significantly decreased the production of pro-inflammatory biomarkers of in response to P...
January 13, 2017: Cytokine
https://www.readbyqxmd.com/read/28092793/high-dilutions-of-antimony-modulate-cytokines-production-and-macrophage-leishmania-l-amazonensis-interaction-in-vitro
#3
Fabiana Rodrigues de Santana, Luciane C Dalboni, Kátia F Nascimento, Fabiana Toshie Konno, Anuska M Alvares-Saraiva, Michelle S F Correia, Maristela Dutra Correa Bomfim, Renato C V Casarin, Elizabeth C Perez, Maria Anete Lallo, Giovani B Peres, Márcia Dalastra Laurenti, Nilson R Benites, Dorly F Buchi, Leoni Villano Bonamin
BACKGROUND: In previous results mice treated with high dilutions of antimony presented reduction of monocyte migration to the site of infection with increase in B lymphocytes population in the local lymph node. AIMS: To know the mechanisms involved, a series of in vitro studies was done, using co-cultures of macrophages (RAW 264.7) and Leishmania (L.) amazonensis treated with different dilutions of antimony (Antimonium crudum or AC), in different times. METHODOLOGY: Spreading, phagocytosis, the oxidative activity of macrophages, the viability of free promastigotes and the cytokines/chemokines concentration in the supernatant were evaluated...
January 13, 2017: Cytokine
https://www.readbyqxmd.com/read/28092668/upregulation-of-ret-induces-perineurial-invasion-of-pancreatic-adenocarcinoma
#4
M Amit, S Na'ara, L Leider-Trejo, Y Binenbaum, N Kulish, E Fridman, A Shabtai-Orbach, R J Wong, Z Gil
Tumor spread along nerves, a phenomenon known as perineurial invasion, is common in various cancers including pancreatic ductal adenocarcinoma (PDAC). Neural invasion is associated with poor outcome, yet its mechanism remains unclear. Using the transgenic Pdx-1-Cre/KrasG12D /p53R172H (KPC) mouse model, we investigated the mechanism of neural invasion in PDAC. To detect tissue-specific factors that influence neural invasion by cancer cells, we characterized the perineurial microenvironment using a series of bone marrow transplantation (BMT) experiments in transgenic mice expressing single mutations in the Cx3cr1, GDNF and CCR2 genes...
January 16, 2017: Oncogene
https://www.readbyqxmd.com/read/28092375/postprandial-macrophage-derived-il-1%C3%AE-stimulates-insulin-and-both-synergistically-promote-glucose-disposal-and-inflammation
#5
Erez Dror, Elise Dalmas, Daniel T Meier, Stephan Wueest, Julien Thévenet, Constanze Thienel, Katharina Timper, Thierry M Nordmann, Shuyang Traub, Friederike Schulze, Flurin Item, David Vallois, Francois Pattou, Julie Kerr-Conte, Vanessa Lavallard, Thierry Berney, Bernard Thorens, Daniel Konrad, Marianne Böni-Schnetzler, Marc Y Donath
The deleterious effect of chronic activation of the IL-1β system on type 2 diabetes and other metabolic diseases is well documented. However, a possible physiological role for IL-1β in glucose metabolism has remained unexplored. Here we found that feeding induced a physiological increase in the number of peritoneal macrophages that secreted IL-1β, in a glucose-dependent manner. Subsequently, IL-1β contributed to the postprandial stimulation of insulin secretion. Accordingly, lack of endogenous IL-1β signaling in mice during refeeding and obesity diminished the concentration of insulin in plasma...
January 16, 2017: Nature Immunology
https://www.readbyqxmd.com/read/28092373/chronic-signaling-via-the-metabolic-checkpoint-kinase-mtorc1-induces-macrophage-granuloma-formation-and-marks-sarcoidosis-progression
#6
Monika Linke, Ha Thi Thanh Pham, Karl Katholnig, Thomas Schnöller, Anne Miller, Florian Demel, Birgit Schütz, Margit Rosner, Boris Kovacic, Nyamdelger Sukhbaatar, Birgit Niederreiter, Stephan Blüml, Peter Kuess, Veronika Sexl, Mathias Müller, Mario Mikula, Wolfram Weckwerth, Arvand Haschemi, Martin Susani, Markus Hengstschläger, Michael J Gambello, Thomas Weichhart
The aggregation of hypertrophic macrophages constitutes the basis of all granulomatous diseases, such as tuberculosis or sarcoidosis, and is decisive for disease pathogenesis. However, macrophage-intrinsic pathways driving granuloma initiation and maintenance remain elusive. We found that activation of the metabolic checkpoint kinase mTORC1 in macrophages by deletion of the gene encoding tuberous sclerosis 2 (Tsc2) was sufficient to induce hypertrophy and proliferation, resulting in excessive granuloma formation in vivo...
January 16, 2017: Nature Immunology
https://www.readbyqxmd.com/read/28092336/the-construction-of-expanded-prefabricated-adipose-tissue-epat-using-an-external-volume-expansion-eve-device
#7
Yi Yuan, Shuifa Yang, Yangyan Yi, Jianhua Gao, Feng Lu
BACKGROUND: Multiple studies have demonstrated that mechanical forces promote the growth of adipose tissue. However, the mechanism of adipose tissue regeneration induced by mechanical forces remains unclear. METHODS: In an experiment using rats, prefabricated adipose tissue with a vessel pedicle was expanded using an external volume expansion (EVE) device. The volume of fat flaps was tested at different time points. Cell proliferation and angiogenesis were analyzed using immunofluorescence...
January 12, 2017: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/28091914/altered-liver-proteoglycan-glycosaminoglycan-structure-as-a-manifestation-of-extracellular-matrix-remodeling-upon-bcg-induced-granulomatosis-in-mice
#8
L B Kim, V A Shkurupy, A N Putyatina
Experimental BCG-induced granulomatosis in mice was used to study changes in the dynamics of individual liver proteoglycan components reflecting phasic extracellular matrix remodeling, determined by the host-parasite interaction and associated with granuloma development. In the early BCG-granulomatosis period, the increase in individual proteoglycan components promotes granuloma formation, providing conditions for mycobacteria adhesion to host cells, migration of phagocytic cells from circulation, and cell-cell interaction leading to granuloma development and fibrosis...
January 14, 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28091876/hypermethylation-of-ifn-%C3%AE-in-oral-cancer-tissues
#9
Songbo Tian, Chunyang Jiang, Xiaoqin Liu, Sheng Xu, Zhiyong Zhang, Huizhen Chen, Yinghuai Zhang, Yanping Liu, Dong Ma
OBJECTIVES: The study aimed to evaluate the methylation pattern of the interferon-gamma (IFN-γ) gene in oral cancer tissues compared with normal and benign oral disease tissues. MATERIALS AND METHODS: The oral tissues were gained from the patients of 85 cases of oral squamous cell carcinoma (OSCC), 47 cases of oral dysplastic lesions, and 53 normal biopsies. IFN -γ methylation in oral tissues was verified through methylation-specific polymerase chain reaction (PCR) and DNA sequencing analyses, and the expression levels of IFN-γ messenger RNA (mRNA) and protein were detected using real-time reverse transcription (RT)-PCR and enzyme-linked immunosorbent assays, respectively...
January 13, 2017: Clinical Oral Investigations
https://www.readbyqxmd.com/read/28091823/skeletal-muscle-inflammation-and-atrophy-in-heart-failure
#10
Kory J Lavine, Oscar L Sierra
Heart failure represents a systemic disease with profound effects on multiple peripheral tissues including skeletal muscle. Within the context of heart failure, perturbations in skeletal muscle physiology, structure, and function strongly contribute to exercise intolerance and the morbidity of this devastating disease. There is growing evidence that chronic heart failure imparts specific pathological changes within skeletal muscle beds resulting in muscle dysfunction and tissue atrophy. Mechanistically, systemic and local inflammatory responses drive critical aspects of this pathology...
January 14, 2017: Heart Failure Reviews
https://www.readbyqxmd.com/read/28091624/paracrine-cross-talk-between-skeletal-muscle-and-macrophages-in-exercise-by-pgc-1%C3%AE-controlled-bnp
#11
Regula Furrer, Petra S Eisele, Alexander Schmidt, Markus Beer, Christoph Handschin
Activation of resident and infiltrating immune cells is a central event in training adaptation and other contexts of skeletal muscle repair and regeneration. A precise orchestration of inflammatory events in muscle fibers and immune cells is required after recurrent contraction-relaxation cycles. However, the mechanistic aspects of this important regulation remain largely unknown. We now demonstrate that besides a dominant role in controlling cellular metabolism, the peroxisome proliferator-activated receptor γ co-activator 1α (PGC-1α) also has a profound effect on cytokine expression in muscle tissue...
January 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28091604/polyglucose-nanoparticles-with-renal-elimination-and-macrophage-avidity-facilitate-pet-imaging-in-ischaemic-heart-disease
#12
Edmund J Keliher, Yu-Xiang Ye, Gregory R Wojtkiewicz, Aaron D Aguirre, Benoit Tricot, Max L Senders, Hannah Groenen, Francois Fay, Carlos Perez-Medina, Claudia Calcagno, Giuseppe Carlucci, Thomas Reiner, Yuan Sun, Gabriel Courties, Yoshiko Iwamoto, Hye-Yeong Kim, Cuihua Wang, John W Chen, Filip K Swirski, Hsiao-Ying Wey, Jacob Hooker, Zahi A Fayad, Willem J M Mulder, Ralph Weissleder, Matthias Nahrendorf
Tissue macrophage numbers vary during health versus disease. Abundant inflammatory macrophages destruct tissues, leading to atherosclerosis, myocardial infarction and heart failure. Emerging therapeutic options create interest in monitoring macrophages in patients. Here we describe positron emission tomography (PET) imaging with (18)F-Macroflor, a modified polyglucose nanoparticle with high avidity for macrophages. Due to its small size, Macroflor is excreted renally, a prerequisite for imaging with the isotope flourine-18...
January 16, 2017: Nature Communications
https://www.readbyqxmd.com/read/28091553/shifts-in-the-fluorescence-lifetime-of-egfp-during-bacterial-phagocytosis-measured-by-phase-sensitive-flow-cytometry
#13
Wenyan Li, Kevin D Houston, Jessica P Houston
Phase-sensitive flow cytometry (PSFC) is a technique in which fluorescence excited state decay times are measured as fluorescently labeled cells rapidly transit a finely focused, frequency-modulated laser beam. With PSFC the fluorescence lifetime is taken as a cytometric parameter to differentiate intracellular events that are challenging to distinguish with standard flow cytometry. For example PSFC can report changes in protein conformation, expression, interactions, and movement, as well as differences in intracellular microenvironments...
January 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28091532/development-of-the-neuroimmune-modulator-ibudilast-for-the-treatment-of-alcoholism-a-randomized-placebo-controlled-human-laboratory-trial
#14
Lara A Ray, Spencer Bujarski, Steve Shoptaw, Daniel Jo Roche, Keith Heinzerling, Karen Miotto
Current directions in medication development for alcohol use disorder (AUD) emphasize the need to identify novel molecular targets and efficiently screen new compounds aimed at those targets. Ibudilast (IBUD) is a neuroimmune modulator that inhibits phosphodiesterases -4 and -10 and macrophage migration inhibitory factor and was recently found to reduce alcohol intake in rats by approximately 50%. To advance medications development for AUD, the present study consists of a randomized, crossover, double-blind, placebo-controlled laboratory study of IBUD in non-treatment seeking individuals with current (ie, past month) mild-to-severe AUD...
January 16, 2017: Neuropsychopharmacology: Official Publication of the American College of Neuropsychopharmacology
https://www.readbyqxmd.com/read/28091516/osteopontin-regulates-macrophage-activation-and-osteoclast-formation-in-hypertensive-patients-with-vascular-calcification
#15
Qian Ge, Cheng-Chao Ruan, Yu Ma, Xiao-Feng Tang, Qi-Hong Wu, Ji-Guang Wang, Ding-Liang Zhu, Ping-Jin Gao
Vascular calcification (VC) is a highly regulated ectopic mineral deposition process involving immune cell infiltration in the vasculatures, which has been recognized to be promoted by hypertension. The matricellular glycoprotein osteopontin (OPN) is strongly induced in myeloid cells as a potential inflammatory mediator of vascular injury. This study aims to examine whether OPN is involved in the regulation of macrophage activation and osteoclast formation in hypertensive subjects with VC. We firstly found an increased proportion of CD11c+CD163- pro-inflammatory peripheral monocytes in hypertensive subjects with VC compared to those without VC by flow cytometric analysis...
January 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28090808/biomaterial-encapsulation-is-enhanced-in-the-early-stages-of-the-foreign-body-reaction-during-conditional-macrophage-depletion-in-transgenic-mafia-mice
#16
Ruud A Bank, Jurjen Zandstra, Hilde Room, Arjen H Petersen, Sander Maarten van Putten
Macrophages are pivotal cells during the foreign body reaction, as they orchestrate the pro-inflammatory microenvironment inside and around biomaterials by secretion of inflammatory mediators. Furthermore they are responsible for the degradation of biomaterials and are thought to instruct the fibroblasts that generate a fibrous capsule around implanted biomaterials. In this study we investigated the events during the FBR when macrophages are not present. Hexamethylenediisocyanate cross-linked collagen scaffolds were implanted in "Macrophage Fas-Induced Apoptosis" (MaFIA) mice, which allow "on demand" macrophage depletion...
January 15, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28090719/diversity-oriented-synthesis-of-cyclic-azapeptides-by-a-3-macrocyclization-provides-high-affinity-cd36-modulating-peptidomimetics
#17
Jinqiang Zhang, Mukandila Mulumba, Huy Ong, William D Lubell
Macrocyclization has enabled the use of peptides in drug discovery creating a need for methods to synthesize diverse peptide macrocycles. Azapeptides have advanced to clinically used drugs, however, few cyclic azapeptides have been studied. A multiple component "A(3) -macrocyclization" strategy is described for the preparation of diverse cyclic azapeptides and is demonstrated by the synthesis of 15 growth hormone releasing hormone-6 (GHRP-6) analogs. Certain cyclic aza-GHRP-6 analogs exhibited unprecedented affinity for the CD36 receptor, and capacity to modulate Toll-like receptor agonist-induced overproduction of nitric oxide, and reduce pro-inflammatory cytokine and chemokine production in macrophages...
January 16, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28090688/exosomal-mir-146a-contributes-to-the-enhanced-therapeutic-efficacy-of-il-1%C3%AE-primed-mesenchymal-stem-cells-against-sepsis
#18
Yuxian Song, Huan Dou, Xiujun Li, Xiaoyin Zhao, Yi Li, Dan Liu, Jianjian Ji, Fei Liu, Liang Ding, Yanhong Ni, Yayi Hou
Improving the immunomodulatory efficacy of mesenchymal stem cells (MSCs) through pretreatment with pro-inflammatory cytokines is an evolving field of investigation. However, the underlying mechanisms have not been fully clarified. Here, we pretreated human umbilical cord-derived MSCs with interleukin-1β (IL-1β) and evaluated their therapeutic effects in a cecal ligation and puncture (CLP)-induced sepsis model. We found that systemic administration of IL-1β-pretreated MSCs (βMSCs) ameliorated the symptoms of murine sepsis more effectively and increased the survival rate compared with naïve MSCs...
January 16, 2017: Stem Cells
https://www.readbyqxmd.com/read/28090670/an-endoplasmic-reticulum-protein-nogo-b-facilitates-alcoholic-liver-disease-through-regulation-of-kupffer-cell-polarization
#19
Jin-Kyu Park, Mingjie Shao, Moon Young Kim, Soon Koo Baik, Mee Yon Cho, Teruo Utsumi, Ayano Satoh, Xinsho Ouyang, Chuhan Chung, Yasuko Iwakiri
Background Nogo-B (Reticulon 4B) is an endoplasmic reticulum (ER) resident protein that regulates ER structure and function. Since ER stress is known to induce M2 macrophage polarization, we examined whether Nogo-B regulates M1/M2 polarization of Kupffer cells and alters the pathogenesis of alcoholic liver disease (ALD). Methods M1 and M2 phenotypes were assessed in relation to Nogo-B expression and disease severity in liver specimens from ALD patients (NCT01875211). Liver specimens from wild-type (WT) and Nogo-B knockout (KO) mice fed control or Lieber-DeCarli ethanol liquid diet (5% ethanol) for 6 weeks were analyzed for liver injury and steatosis...
January 16, 2017: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
https://www.readbyqxmd.com/read/28090638/late-gestational-intermittent-hypoxia-induces-metabolic-and-epigenetic-changes-in-male-adult-offspring-mice
#20
Abdelnaby Khalyfa, Rene Cortese, Zhuanhong Qiao, Honggang Ye, Riyue Bao, Jorge Andrade, David Gozal
Pregnancy, particularly late gestation, has been associated with a relatively high prevalence of obstructive sleep apnea (OSA). Intermittent hypoxia (IH), a hallmark of OSA, could impose significant long-term effects on somatic growth, energy homeostasis, and metabolic function in offspring. We hypothesized that IH during late pregnancy (LG-IH) may increase the propensity for metabolic dysregulation and obesity in adult offspring via epigenetic modifications. Time-pregnant female C57BL/6 mice were exposed to LG-IH or room air (LG-RA) during days 13-18 of gestation...
January 15, 2017: Journal of Physiology
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