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https://www.readbyqxmd.com/read/27921414/the-effect-of-autologous-serum-therapy-on-disease-severity-in-patients-with-chronic-urticaria
#1
Farhad Abolnezhadian, Soheila Alyasin, Reza Amin, Maryam Babaei
Limited evidence has been obtained concerning the beneficial effects of autologous serum therapy in treatment of skin disorders particularly chronic urticaria. In the present study, we have assessed the effect of this treatment method in patients with chronic urticaria (CU). This randomized single-blind controlled trial was performed on fifty consecutive patients with chronic urticaria. The patients were randomly assigned to receive autologous serum (as the case group, n=35) or normal saline (as the control group, n=15) and treated with monthly autologous serum therapy or normal saline for 6 months...
August 2016: Iranian Journal of Allergy, Asthma, and Immunology
https://www.readbyqxmd.com/read/27921402/developing-a-rat-model-of-dilated-cardiomyopathy-with-improved-survival
#2
Li-Juan Shen, Shu Lu, Yong-Hua Zhou, Lan Li, Qing-Min Xing, Yong-Liang Xu
To compare the continuous infusion and intermittent bolus injection administration protocols of doxorubicin (Dox) under the same cumulative dose (12 mg/kg), and establish a rat dilated cardiomyopathy model with improved survival, a total of 150 Sprague-Dawley (SD) rats were divided into three groups: a control group, administered with normal saline; a Dox 1 group, administration twice a week at 1 mg/kg; a Dox 2, administration once a week at 2 mg/kg. Mortality rates in the Dox 1 and Dox 2 groups were 22% and 48%, respectively (P<0...
2016: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/27921022/the-protective-effect-of-hydro-alcoholic-extract-of-mangrove-avicennia-marina-l-leaves-on-kidney-injury-induced-by-carbon-tetrachloride-in-male-rats
#3
Naser Mirazi, Seyedeh-Nahid Movassagh, Mahmoud Rafieian-Kopaei
BACKGROUND: Materials can cause liver and kidney damage which carbon tetrachloride is one of these substances. Medicinal plants and their essential oils and extracts have been used to a large extent as drugs to better control and management of kidney diseases. OBJECTIVES: The aim of this study was to investigate the effect of hydro-alcoholic extract of Avicennia marina leaves in the treatment of renal toxicity induced by carbon tetrachloride. METHODS: Forty-two male rats were randomly divided into 6 groups (n = 7): control (taking normal saline, 0...
October 2016: Journal of Nephropathology
https://www.readbyqxmd.com/read/27920821/protective-effect-of-vitamin-c-vitamin-b12-and-omega-3-on-lead-induced-memory-impairment-in-rat
#4
Saeedeh Alsadat Moosavirad, Mohammad Rabbani, Mohammad Sharifzadeh, Ali Hosseini-Sharifabad
Lead belongs to the heavy metal group and is considered as an environmental contaminant. Acute or chronic contact to lead can change the physiological function of human organs. One of the most important disorders following the lead exposure is neurotoxicity. Lead neurotoxicity consists of the neurobehavioral disturbances like cognitive impairment. The aim of the current study is to evaluate the possible protective effect of vitamin C (Vit C), vitamin B12 (Vit B12), omega 3 (ω-3), or their combination on the lead-induced memory disorder...
October 2016: Research in Pharmaceutical Sciences
https://www.readbyqxmd.com/read/27920763/microbial-activity-response-to-solar-radiation-across-contrasting-environmental-conditions-in-salar-de-huasco-northern-chilean-altiplano
#5
Klaudia L Hernández, Beatriz Yannicelli, Lasse M Olsen, Cristina Dorador, Eduardo J Menschel, Verónica Molina, Francisco Remonsellez, Martha B Hengst, Wade H Jeffrey
In high altitude environments, extreme levels of solar radiation and important differences of ionic concentrations over narrow spatial scales may modulate microbial activity. In Salar de Huasco, a high-altitude wetland in the Andean mountains, the high diversity of microbial communities has been characterized and associated with strong environmental variability. Communities that differed in light history and environmental conditions, such as nutrient concentrations and salinity from different spatial locations, were assessed for bacterial secondary production (BSP, (3)H-leucine incorporation) response from short-term exposures to solar radiation...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27920761/diversity-and-distribution-of-phenol-oxidase-producing-fungi-from-soda-lake-and-description-of-curvularia-lonarensis-sp-nov
#6
Rahul Sharma, Om Prakash, Mahesh S Sonawane, Yogesh Nimonkar, Priyanka B Golellu, Rohit Sharma
Soda lake is hyper alkaline and saline habitat located in closed craters with high evaporation rate. In current study fungal diversity from water and sediment samples of a soda lake (Lonar lake) located in Buldhana district of Maharashtra, India was investigated using extensive culturomics approach and mimicking the natural conditions of Lonar lake in culture media. A total of 104 diverse isolates of extremophilic fungi were recovered from this study and phylogenetically characterized by internal transcribed spacer (ITS) region sequencing...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27920667/double-in-situ-hybridization-for-micrornas-and-mrnas-in-brain-tissues
#7
Atsushi Kasai, Sora Kakihara, Hiroki Miura, Ryo Okada, Atsuko Hayata-Takano, Keisuke Hazama, Misaki Niu, Norihito Shintani, Takanobu Nakazawa, Hitoshi Hashimoto
MicroRNAs (miRNAs) participate in a variety of functions in the brain. Understanding the in vivo localization of miRNAs is an important step for uncovering their roles in brain function. However, the in situ detection of low-abundance miRNAs in brain tissues remains difficult and requires extensive optimization of in situ hybridization (ISH) protocols in individual laboratories. Thus, detailed information regarding experimental conditions would serve as a useful reference for researchers in this field. Here, we investigated and summarized the effects of adjusting a series of critical steps, including tissue fixation, probe accessibility and hybridization stringency, to standardize the currently used miRNA ISH procedures...
2016: Frontiers in Molecular Neuroscience
https://www.readbyqxmd.com/read/27920522/incorporation-of-heparin-binding-proteins-into-preformed-dextran-sulfate-chitosan-nanoparticles
#8
Paula Zaman, Julia Wang, Adam Blau, Weiping Wang, Tina Li, Daniel S Kohane, Joseph Loscalzo, Ying-Yi Zhang
Incorporation of proteins into dextran sulfate (DS)-chitosan (CS) nanoparticles (DSCS NPs) is commonly performed using entrapment procedures, in which protein molecules are mixed with DS and CS until particle formation occurs. As DS is an analog of heparin, the authors examined whether proteins could be directly incorporated into preformed DSCS NPs through a heparin binding domain-mediated interaction. The authors formulated negatively-charged DSCS NPs, and quantified the amount of charged DS in the outer shell of the particles...
2016: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/27920429/nitric-oxide-no-mediates-the-inhibition-of-form-deprivation-myopia-by-atropine-in-chicks
#9
Brittany J Carr, William K Stell
Myopia is the most common childhood refractive disorder. Atropine inhibits myopia progression, but its mechanism is unknown. Here, we show that myopia-prevention by atropine requires production of nitric oxide (NO). Form-deprivation myopia (FDM) was induced in week-old chicks by diffusers over the right eye (OD); the left eye (OS) remained ungoggled. On post-goggling days 1, 3, and 5, OD received intravitreally 20 µL of phosphate-buffered saline (vehicle), or vehicle plus: NO source: L-arginine (L-Arg, 60-6,000 nmol) or sodium nitroprusside (SNP, 10-1,000 nmol); atropine (240 nmol); NO inhibitors: L-NIO or L-NMMA (6 nmol); negative controls: D-Arg (10 µmol) or D-NMMA (6 nmol); or atropine plus L-NIO, L-NMMA, or D-NMMA; OS received vehicle...
December 2016: Scientific Reports
https://www.readbyqxmd.com/read/27919624/metabolic-fate-of-glucose-in-rats-with-traumatic-brain-injury-and-pyruvate-or-glucose-treatments-a-nmr-spectroscopy-study
#10
Katsunori Shijo, Richard L Sutton, Sima S Ghavim, Neil G Harris, Brenda L Bartnik-Olson
Administration of sodium pyruvate (SP; 9.08 μmol/kg, i.p.), ethyl pyruvate (EP; 0.34 μmol/kg, i.p.) or glucose (GLC; 11.1 μmol/kg, i.p.) to rats after unilateral controlled cortical impact (CCI) injury has been reported to reduce neuronal loss and improve cerebral metabolism. In the present study these doses of each fuel or 8% saline (SAL; 5.47 nmoles/kg) were administered immediately and at 1, 3, 6 and 23 h post-CCI. At 24 h all CCI groups and non-treated Sham injury controls were infused with [1,2 (13)C] glucose for 68 min (13)C nuclear magnetic resonance (NMR) spectra were obtained from cortex + hippocampus tissues from left (injured) and right (contralateral) hemispheres...
December 2, 2016: Neurochemistry International
https://www.readbyqxmd.com/read/27919595/the-starting-point-for-bisphosphonate-related-osteonecrosis-of-the-jaw-alveolar-bone-or-oral-mucosa-a-randomized-controlled-experimental-study
#11
Mohammad Zandi, Arash Dehghan, Pejman Janbaz, Hamid Malekzadeh, Payam Amini
OBJECTIVE: Although over a decade has passed since first introduction of BRONJ, the exact pathophysiology of this disease is still unclear. The present experimental study aimed to determine whether the oral mucosa or alveolar bone serves as the starting point for BRONJ development. SUBJECTS AND METHODS: Sixty male Wistar rats were randomly assigned into study and control groups (each, n = 30), and received intraperitoneal injection of 0.06 mg/kg zoledronate and saline, respectively, once a week for 12 weeks...
November 2, 2016: Journal of Cranio-maxillo-facial Surgery
https://www.readbyqxmd.com/read/27919529/three-step-effluent-chlorination-increases-disinfection-efficiency-and-reduces-dbp-formation-and-toxicity
#12
Yu Li, Xiangru Zhang, Mengting Yang, Jiaqi Liu, Wanxin Li, Nigel J D Graham, Xiaoyan Li, Bo Yang
Chlorination is extensively applied for disinfecting sewage effluents, but it unintentionally generates disinfection byproducts (DBPs). Using seawater for toilet flushing introduces a high level of bromide into domestic sewage. Chlorination of sewage effluent rich in bromide causes the formation of brominated DBPs. The objectives of achieving a disinfection goal, reducing disinfectant consumption and operational costs, as well as diminishing adverse effects to aquatic organisms in receiving water body remain a challenge in sewage treatment...
December 2, 2016: Chemosphere
https://www.readbyqxmd.com/read/27919272/enhanced-lipid-and-biomass-production-by-a-newly-isolated-and-identified-marine-microalga
#13
Mouna Dammak, Sandra Mareike Haase, Ramzi Miladi, Faten Ben Amor, Mohamed Barkallah, David Gosset, Chantal Pichon, Bernhard Huchzermeyer, Imen Fendri, Michel Denis, Slim Abdelkafi
BACKGROUND: The increasing demand for microalgae lipids as an alternative to fish has encouraged researchers to explore oleaginous microalgae for food uses. In this context, optimization of growth and lipid production by the marine oleaginous V2-strain-microalgae is of great interest as it contains large amounts of mono-unsaturated (MUFAs) and poly-unsaturated fatty acids (PUFAs). METHODS: In this study, the isolated V2 strain was identified based on 23S rRNA gene...
December 5, 2016: Lipids in Health and Disease
https://www.readbyqxmd.com/read/27918282/conditioned-object-preference-an-alternative-approach-to-measuring-reward-learning-in-rats
#14
Bruce C Kennedy, Maulika Kohli, Jamie J Maertens, Paulina S Marell, Jonathan C Gewirtz
Pavlovian conditioned approach behavior can be directed as much toward discrete cues as it is toward the environmental contexts in which those cues are encountered. The current experiments characterized a tendency of rats to approach object cues whose prior exposure had been paired with reward (conditioned object preference, COP). To demonstrate the phenomenon, rats were conditioned to associate cocaine or saline with two different objects. Rats acquired a preference, assessed using investigation times directed toward each object, for the cocaine-paired object following conditioning...
November 2016: Learning & Memory
https://www.readbyqxmd.com/read/27918144/a-novel-rat-tail-discitis-model-using-bioluminescent-staphylococcus-aureus
#15
Phillip A Bostian, Jonathan M Karnes, Shari Cui, Lisa J Robinson, Scott D Daffner, Michelle R Witt, Sanford E Emery
Management of spondylodiscitis is a challenging clinical problem requiring medical and surgical treatment strategies. The purpose of this study was to establish a rat model of spondylodiscitis that utilizes bioluminescent Staphylococcus aureus, thus permitting in-vivo surveillance of infection intensity. Inocula of the bioluminescent S. aureus strain XEN36 were created in concentrations of 10(2) CFU/0.1 mL, 10(4) CFU/0.1 mL, and 10(6) CFU/0.1 mL. Three groups of rats were injected with the bacteria in the most proximal intervertebral tail segment...
December 5, 2016: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://www.readbyqxmd.com/read/27918123/chrysomya-bezziana-diptera-calliphoridae-infestation-case-report-of-three-dogs-in-malaysia-treated-with-spinosad-milbemycin
#16
Hock Siew Han, Reuben Sharma, John Jeffery, Chiara Noli
BACKGROUND: Infestation of wounds with the larvae of Callophorid flies is relatively common in countries where these parasites are found. The most common species associated with infections in Southeast Asia is Chrysomya bezziana (Ch. bezziana), the Old World screw worm. Treatment consists of either subcutaneous injection of ivermectin or oral administration of nitenpyram combined with aggressive tissue debridement under general anaesthesia. OBJECTIVES: To describe the treatment of cutaneous myiasis in three dogs caused by the larvae of Ch...
December 5, 2016: Veterinary Dermatology
https://www.readbyqxmd.com/read/27918101/symptomatic-absorption-of-isotonic-saline-during-transcervical-endometrial-resection
#17
J Johansson, M Lindahl, E Gyllencreutz, R G Hahn
No abstract text is available yet for this article.
January 2017: Acta Anaesthesiologica Scandinavica
https://www.readbyqxmd.com/read/27918023/reactive-convective-dissolution-in-a-porous-medium-the-storage-of-carbon-dioxide-in-saline-aquifers
#18
Parama Ghoshal, Min Chan Kim, Silvana S S Cardoso
We quantify the destabilising effect of a first-order chemical reaction on the fingering instability of a diffusive boundary layer in a porous medium. Using scaling, we show that the dynamics of such a reactive boundary layer is fully determined by two dimensionless groups: Da/Ra(2), which measures the timescale for convection compared to those for reaction and diffusion; and βC/βA, which reflects the density change induced by the product relative to that of the diffusing solute. Linear stability and numerical results for βC/βA in the range 0-10 and Da/Ra(2) in the range 0-0...
December 5, 2016: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/27917816/effect-of-ankaferd-blood-stopper-on-muscle-healing
#19
Murat Uygun, Osman Y Yavuz, İsmail Uraş, İbrahim G Duman, Hacer Haltaş, Mahmut Kömürcü
PURPOSE: Ankaferd Blood Stopper (ABS), which is a standardized mixture of herbal extracts obtained from five plants, has been proven as an efficient hemostatic agent and is still used in emergency situations. It is not known exactly if decreased bleeding has positive or negative effects on muscle healing and fibrosis, so the purpose of this study was to test the effect of ABS on muscle healing and morphology. METHODS: A total of 66 outbred Wistar rats were divided into three control and three experimental subgroups...
December 1, 2016: Clinical and Investigative Medicine. Médecine Clinique et Experimentale
https://www.readbyqxmd.com/read/27917814/protective-effect-of-intravesical-platelet-rich-plasma-on-cyclophosphamide-induced-hemorrhagic-cystitis
#20
E Ozyuvali, M E Yildirim, T Yaman, B Kosem, E Cimentepe
PURPOSE: Hemorrhagic cystitis (HC) is the most common urotoxic side effect of cyclophosphamide (CYP). Platelet rich plasma (PRP) plays an important role in wound healing and inflammatory responses. The aim of this study was to investigate the efficacy of intravesical PRP at treatment of interstitial cystitis (IC). METHODS: Female rats (n=24) were used. IC was induced by intraperitoneal injection of cyclophosphamide (CYP) and rats were randomly allocated to one of four groups (n = 6 per group): A control group; a sham group with saline (75 mg/kg; i...
December 1, 2016: Clinical and Investigative Medicine. Médecine Clinique et Experimentale
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