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https://www.readbyqxmd.com/read/28454052/a-multi-biomarker-approach-in-scallop-chlamys-farreri-to-assess-the-impact-of-contaminants-in-qingdao-coastal-area-of-china
#1
Ruiming Guo, Luqing Pan, Rongwang Ji
A multi-biomarker approach was carried out to classify the environmental quality and the adverse effects of contaminants on scallop Chlamys farreri. The scallops were collected from three sampling stations in Qingdao coastal area of China in March, May, August and October of 2015. A suite of environmental factors and biomarkers, including temperature, salinity, pH, the concentrations of polycyclic aromatic hydrocarbons (PAHs), tetrabromobisphenol A (TBBPA) and metals (Cr, Mn, Cu, Zn, Cd, Pb, As) in seawater and soft tissue, mRNA expression of aryl hydrocarbon receptor (AhR) and P-glycoprotein (P-gp), 7-ethoxyresorufin O-deethylase (EROD), glutathione-S-transferase (GST), uridine-diphosphate-glucuronyl-transferase (UGT), sulfotransferase (SULT), metallothionein (MT), Superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT), lipid peroxidation (LPO) and protein carbonyl (PC) contents and DNA strand breaks, were measured in the gill and digestive gland...
April 25, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28453535/plasma-amino-acids-and-metabolic-profiling-of-dairy-cows-in-response-to-a-bolus-duodenal-infusion-of-leucine
#2
Hassan Sadri, Dirk von Soosten, Ulrich Meyer, Jeannette Kluess, Sven Dänicke, Behnam Saremi, Helga Sauerwein
Leucine (Leu), one of the three branch chain amino acids, acts as a signaling molecule in the regulation of overall amino acid (AA) and protein metabolism. Leucine is also considered to be a potent stimulus for the secretion of insulin from pancreatice β-cells. Our objective was to study the effects of a duodenal bolus infusion of Leu on insulin and glucagon secretion, on plasma AA concentrations, and to do a metabolomic profiling of dairy cows as compared to infusions with either glucose or saline. Six duodenum-fistulated Holstein cows were studied in a replicated 3 × 3 Latin square design with 3 periods of 7 days, in which the treatments were applied at the end of each period...
2017: PloS One
https://www.readbyqxmd.com/read/28453295/postinjury-exercise-and-platelet-rich-plasma-therapies-improve-skeletal-muscle-healing-in-rats-but-are-not-synergistic-when-combined
#3
Paola Contreras-Muñoz, Joan Ramon Torrella, Xavier Serres, David Rizo-Roca, Meritxell De la Varga, Ginés Viscor, Vicente Martínez-Ibáñez, José Luis Peiró, Tero A H Järvinen, Gil Rodas, Mario Marotta
BACKGROUND: Skeletal muscle injuries are the most common sports-related injury and a major concern in sports medicine. The effect of platelet-rich plasma (PRP) injections on muscle healing is still poorly understood, and current data are inconclusive. PURPOSE: To evaluate the effects of an ultrasound-guided intramuscular PRP injection, administered 24 hours after injury, and/or posttraumatic daily exercise training for 2 weeks on skeletal muscle healing in a recently established rat model of skeletal muscle injury that highly mimics the muscle trauma seen in human athletes...
April 1, 2017: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/28453275/dielectric-response-of-cytoplasmic-water-and-its-connection-to-the-vitality-of-human-red-blood-cells-ii-the-influence-of-storage
#4
Evgeniya Levy, Marcelo David, Gregory Barshtein, Saul Yedgar, Leonid Livshits, Paul Ben Ishai, Yuri Feldman
Maintaining an appropriate inventory of packaged blood products is a critical part of modern medicine. Consequently, the assessment of red blood cell (RBC) functionality is instrumental for the monitoring of the quality of stored RBC (sRBC) in the blood bank. We present a comprehensive study of sRBC lesion kinetics in SAGM (Saline, Adenine, Glucose, Mannitol) solution, using microwave dielectric spectroscopy (0.5 GHz - 50 GHz) and cell deformability. As part of the research we have isolated the microwave dielectric response of cytoplasmic water in sRBC...
April 28, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28449541/nanoscale-observation-of-dehydration-process-in-phema-hydrogel-structure
#5
Kordian Chamerski, Witold Korzekwa, Jacek Filipecki, Olha Shpotyuk, Marcin Stopa, Piotr Jeleń, Maciej Sitarz
One of the most important field of interest in respect to hydrogel materials is their capability to water storage. The problem mentioned above plays an important role regarding to diffusion of fluid media containing nanoparticles, what is very useful in biomedical applications, such as artificial polymeric implants, drug delivery systems or tissue engineering.In presented work, dehydration process in hydrogels used in ophthalmology as intraocular lenses was observed. Before measurements studied materials were immersed in deionized water and saline solution to obtain equilibrium swelling state...
December 2017: Nanoscale Research Letters
https://www.readbyqxmd.com/read/28449460/role-of-bronchoprovocation-tests-in-identifying-exercise-induced-bronchoconstriction-in-a-non-athletic-population-a-pilot-study
#6
Jessica H Y Tan, Wui Mei Chew, Therese S Lapperre, Gan Liang Tan, Chian Min Loo, Mariko S Koh
BACKGROUND: Studies on diagnostic tests for exercise-induced bronchoconstriction (EIB) have centered around the asthmatic and elite athletic population. Traditionally, the exercise challenge test (ET) was recommended to assess EIB. We aimed to compare the performance of surrogate testing, mainly the hypertonic saline (HS) test, and methacholine challenge test (MCT) versus ET in identifying EIB among non-athletic subjects. METHODS: We prospectively recruited subjects who did not have confirmed active asthma, but who reported exercise-induced dyspnoea...
March 2017: Journal of Thoracic Disease
https://www.readbyqxmd.com/read/28449313/neurovascular-unit-alteration-in-somatosensory-cortex-and-enhancement-of-thermal-nociception-induced-by-amphetamine-involves-central-at1-receptor-activation
#7
Victoria Belén Occhieppo, Natalia Andrea Marchese, Iara Diamela Rodríguez, Osvaldo Martín Basmadjian, Gustavo Baiardi, Claudia Bregonzio
The use of psychostimulants, such as amphetamine (Amph), is associated with inflammatory processes, involving glia and vasculature alterations. Brain Angiotensin II (Ang II), through AT1 -receptors (AT1 -R), modulates neurotransmission and plays a crucial role in inflammatory responses in brain vasculature and glia. Our aim for the present work was to evaluate the role of AT1 -R in long-term alterations induced by repeated exposure to Amph. Astrocyte reactivity, neuronal survival and brain microvascular network were analyzed at the somatosensory cortex...
April 27, 2017: European Journal of Neuroscience
https://www.readbyqxmd.com/read/28449280/a-randomised-controlled-trial-of-placebo-droperidol-or-ondansetron-to-prevent-nausea-and-vomiting-after-tonsillectomy-in-children-receiving-dexamethasone
#8
P Flubacher, N Fournier, J Cherpillod, F Waridel, M Nydegger, E Albrecht
We tested whether prophylactic droperidol and ondansetron, in combination with a moderate dose of dexamethasone, were equally effective in reducing nausea and vomiting after tonsillectomy in children and that both were superior to saline with dexamethasone. We randomly allocated 300 children to intravenous saline, droperidol 10 μg.kg(-1) or ondansetron 150 μg.kg(-1) , after induction of anaesthesia and the administration of intravenous dexamethasone 250 μg.kg(-1) . The rates (95%CI) of nausea or vomiting within 24 postoperative hours were: 42/91 after saline, 46% (36%-57%); 43/87 after droperidol, 49% (39%-60%); reduced to 18/84 by ondansetron, 21% (13%-32%), p < 0...
April 27, 2017: Anaesthesia
https://www.readbyqxmd.com/read/28448913/temporal-characterization-of-flowback-and-produced-water-quality-from-a-hydraulically-fractured-oil-and-gas-well
#9
James Rosenblum, Andrew W Nelson, Bridger Ruyle, Michael K Schultz, Joseph N Ryan, Karl G Linden
This study examined water quality, naturally-occurring radioactive materials (NORM), major ions, trace metals, and well flow data for water used and produced from start-up to operation of an oil and gas producing hydraulically-fractured well (horizontal) in the Denver-Julesburg (DJ) Basin in northeastern Colorado. Analysis was conducted on the groundwater used to make the fracturing fluid, the fracturing fluid itself, and nine flowback/produced water samples over 220days of operation. The chemical oxygen demand of the wastewater produced during operation decreased from 8200 to 2500mg/L, while the total dissolved solids (TDS) increased in this same period from 14,200 to roughly 19,000mg/L...
April 24, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28448816/protective-effect-of-artemisinin-on-chronic-alcohol-induced-liver-damage-in-mice
#10
Xiaoyan Zhao, Liqing Wang, Hao Zhang, Duoduo Zhang, Zhihao Zhang, Jie Zhang
The liver disease related to chronic alcohol consumption is one of the leading causes of death for alcoholics. The efficient drug to ameliorate the alcoholic liver injury was needed urgently. The present study was performed to investigate whether artemisinin possessed the protective effect against chronic alcohol consumption. 50 male Kunming mice were divided into 5 groups: control group (C): 10ml/kg saline+10ml/kg saline, alcohol group (A): 10ml/kg 56%(v/v) alcohol+10ml/kg saline, low dose group of artemisinin (L): 10ml/kg 56%(v/v) alcohol+30mg/kg/day artemisinin, medium dose group of artemisinin (M): 10ml/kg 56%(v/v) alcohol+60mg/kg/day artemisinin, high dose group of artemisinin (H): 10ml/kg 56%(v/v) alcohol+120mg/kg/day artemisinin...
April 13, 2017: Environmental Toxicology and Pharmacology
https://www.readbyqxmd.com/read/28448397/prophylactic-pentazocine-reduces-the-incidence-of-pruritus-after-cesarean-delivery-under-spinal-anesthesia-with-opioids-a-prospective-randomized-clinical-trial
#11
Masato Hirabayashi, Katsushi Doi, Noritaka Imamachi, Tomomune Kishimoto, Yoji Saito
BACKGROUND: The incidence of pruritus after cesarean delivery under spinal anesthesia with opioids is high, ranging from 50% to 100%. Pruritus is difficult to prevent; however, pentazocine has been shown to be an effective treatment. Despite this, the prophylactic effect of pentazocine on pruritus has not been defined. This randomized double-blind trial aimed to evaluate the effect of intraoperative IV pentazocine on the incidence of opioid-induced pruritus within the first 24 hours after administration of neuraxial opioids...
April 24, 2017: Anesthesia and Analgesia
https://www.readbyqxmd.com/read/28448392/a-retrospective-study-to-evaluate-the-effect-of-concentration-of-hypertonic-saline-on-efficacy-and-safety-of-epidural-adhesiolysis
#12
Eun Joo Choi, Yong Jae Yoo, Pyung Bok Lee, Yong-Chul Kim, Sang Chul Lee, Jee Youn Moon
BACKGROUND: Percutaneous epidural adhesiolysis (PEA) is a minimally invasive procedure that is performed to relieve low back and/or lower limb pain secondary to adhesions or scarring in the epidural space that is refractory to conservative treatment. The optimal concentration of hypertonic saline might be an important factor in the safety and efficacy of PEA. We evaluated differences in the efficacy and safety of 2 concentrations of hypertonic saline (5% and 10%) used in lumbar PEA at our institutions in a retrospective study...
April 24, 2017: Anesthesia and Analgesia
https://www.readbyqxmd.com/read/28448273/magnetic-induction-spectroscopy-mis-probe-design-for-cervical-tissue-measurements
#13
Jau-Yi Wang, Timothy Healey, Anthony Barker, Brian Brown, Chris Monk, Dilly Anumba
OBJECTIVE: Gradiometers have the advantage of increasing measuring sensitivity, which is particularly useful in magnetic induction spectroscopy (MIS) for bio-impedance measurements. Traditional gradiometers use a pair of field sensing coils equally distant and on opposite sides of a drive coil, which provides high immunity to interference. In this paper, a ferrite-cored coaxial gradiometer probe of 29 mm diameter has been developed for measuring the impedance spectra of cervical tissues in vivo...
May 2017: Physiological Measurement
https://www.readbyqxmd.com/read/28448180/renal-denervation-decreases-blood-pressure-and-renal-tyrosine-hydroxylase-but-does-not-augment-the-effect-of-hypotensive-drugs
#14
Janusz Skrzypecki, Maciej Gawlak, Tomasz Huc, Paweł Szulczyk, Marcin Ufnal
The effect of renal denervation on the efficacy of antihypertensive drugs has not yet been elucidated. Twenty-week-old spontaneously hypertensive rats were treated with metoprolol, losartan, indapamide, or saline (controls) and assigned to renal denervation or a sham procedure. Acute hemodynamic measurements were performed ten days later. Series showing a significant interaction between renal denervation and the drugs were repeated with chronic telemetry measurements. In the saline series, denervated rats showed a significantly lower mean arterial blood pressure (blood pressure) than the sham-operated rats...
2017: Clinical and Experimental Hypertension: CHE
https://www.readbyqxmd.com/read/28448022/development-of-an-economical-dna-delivery-system-by-acufection-and-its-application-to-skin-research
#15
Yu-Jei Lin, Tsung-Lin Lee, Chia-Chi Ku
Dysregulation of immune response in skin is associated with numerous human skin disorders. Direct transfer of immune-related genes into skin tissue is a fascinating approach to investigate immune modulation of cutaneous inflammation in mouse models of human diseases. Here we present a cost-effective protocol that delivered naked DNA in mouse skin and leads to transgene expression. The method is coined "acufection", denoting acupuncture-mediated DNA transfection. To perform acufection, mouse skin was first infused with DNA in phosphate-buffered saline (PBS) and then pricked lightly with a bundle of acupuncture needles to facilitate the absorption of DNA and transfection into cells...
April 19, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28447793/hydrogen-peroxide-induced-change-in-meat-quality-of-breast-muscle-of-broilers-is-mediated-by-ros-generation-apoptosis-and-autophagy-in-nf-%C3%AE%C2%BAb-signal-pathway
#16
Xiangxing Chen, Lin Zhang, Jiaolong Li, Feng Gao, Guanghong Zhou
We investigated the relationship between meat quality and oxidative damage caused by hydrogen peroxide (H2O2) in the breast muscle of broilers. Moreover, we explored the occurrence of apoptosis and autophagy, as well as the expression of NF-κB in these signaling pathways, to provide evidence of possible oxidative damage mechanisms. The broilers received a basal diet and were randomly divided into five treatments (non-injected control, 0.75% saline-injected, and 2.5%, 5.0%, or 10.0% H2O2-injected treatments; 1...
April 27, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28446851/an-invitro-evaluation-of-the-accuracy-of-two-electronic-apex-locators-to-determine-working-length-in-the-presence-of-various-irrigants
#17
Ashwini B Prasad, Srivastava Harshit, Srivastava Arora Aastha, Raisingani Deepak
BACKGROUND: Cleaning and shaping of root canal system requires various irrigating solutions. The question is whether the presence of irrigation solution alters working length determination using apex locators. The purpose of this study was to compare the accuracy of Root ZX and i-Root apex locator for determining working length in the presence of different irrigating solution. MATERIALS AND METHOD: Eighty extracted single rooted human teeth were used. The teeth were sectioned at Cemento Enamel Junction (CEJ) and actual canal length determined...
September 2016: Ethiopian Journal of Health Sciences
https://www.readbyqxmd.com/read/28446801/genetic-susceptibility-to-salt-sensitive-hypertension-in-a-han-chinese-population-a-validation-study-of-candidate-genes
#18
Zheng Liu, Han Qi, Bin Liu, Kuo Liu, Jingjing Wu, Han Cao, Jie Zhang, Yuxiang Yan, Yan He, Ling Zhang
Salt-sensitive hypertension is a complex disease associated with genetic factors. This study aimed to identify the association between 29 candidate single-nucleotide polymorphisms and salt-sensitive hypertension in a Han Chinese population. Sixty-three participants with salt-sensitive hypertension and 279 controls with salt-resistant hypertension were recruited. A modified Sullivan's acute oral saline load and diuresis shrinkage test was used to detect blood pressure salt sensitivity. Lifestyle risk factors were obtained via a questionnaire...
April 27, 2017: Hypertension Research: Official Journal of the Japanese Society of Hypertension
https://www.readbyqxmd.com/read/28446482/secondary-parkinsonism-in-a-patient-of-psychogenic-polydipsia
#19
Rimesh Pal, Liza Das, Pinaki Dutta, Anil Bhansali
A 44-year-old man presented with history of slurring of speech, slowness in activities, abnormal posturing of the upper limbs and drooling of saliva from the mouth. He had a 5-yearlong history of compulsive water drinking, consuming 12-15 L of water every day. He was earlier evaluated for the same and found to have low serum sodium (126 mEq/L). Presently, he was admitted at a primary care facility with fever and altered sensorium. He was found to have serum sodium of 94 mEq/L. His sensorium improved with hypertonic saline infusion; however, after a lucid interval of 48 hours, he developed florid extrapyramidal symptoms...
April 26, 2017: BMJ Case Reports
https://www.readbyqxmd.com/read/28446241/polyunsaturated-fatty-acid-receptors-gpr40-and-gpr120-are-expressed-in-the-hypothalamus-and-control-energy-homeostasis-and-inflammation
#20
Nathalia R V Dragano, Carina Solon, Albina F Ramalho, Rodrigo F de Moura, Daniela S Razolli, Elisabeth Christiansen, Carlos Azevedo, Trond Ulven, Licio A Velloso
BACKGROUND: The consumption of large amounts of dietary fats is one of the most important environmental factors contributing to the development of obesity and metabolic disorders. GPR120 and GPR40 are polyunsaturated fatty acid receptors that exert a number of systemic effects that are beneficial for metabolic and inflammatory diseases. Here, we evaluate the expression and potential role of hypothalamic GPR120 and GPR40 as targets for the treatment of obesity. METHODS: Male Swiss (6-weeks old), were fed with a high fat diet (HFD, 60% of kcal from fat) for 4 weeks...
April 26, 2017: Journal of Neuroinflammation
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