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https://www.readbyqxmd.com/read/28719947/efficient-liquid-media-for-encystation-of-pathogenic-free-living-amoebae
#1
Hae-Jin Sohn, Heekyoung Kang, Ga-Eun Seo, Jong-Hyun Kim, Suk-Yul Jung, Ho-Joon Shin
Pathogenic Naegleria fowleri, Acanthamoeba castellanii, and Acanthamoeba polyphaga, are distributed worldwide. They are causative agents of primary amoebic meningoencephalitis or acanthamoebic keratitis in humans, respectively. Trophozoites encyst in unfavorable environments, such as exhausted food supply and desiccation. Until recently, the method of N. fowleri encystation used solid non-nutrient agar medium supplemented with heat-inactivated Escherichia coli; however, for the amoebic encystment of Acanthamoeba spp...
June 2017: Korean Journal of Parasitology
https://www.readbyqxmd.com/read/28719628/salt-tolerant-and-sensitive-alfalfa-medicago-sativa-cultivars-have-large-variations-in-defense-responses-to-the-lepidopteran-insect-spodoptera-litura-under-normal-and-salt-stress-condition
#2
Yunting Lei, Qing Liu, Christian Hettenhausen, Guoyan Cao, Qing Tan, Weiye Zhao, Honghui Lin, Jianqiang Wu
In nature, plants are often exposed to multiple stress factors at the same time. Yet, little is known about how plants modulate their physiology to counteract simultaneous abiotic and biotic stresses, such as soil salinity and insect herbivory. In this study, insect performance bioassays, phytohormone measurements, quantification of transcripts, and protein determination were employed to study the phenotypic variations of two alfalfa (Medicago sativa) cultivars in response to insect Spodoptera litura feeding under normal and salt stress condition...
2017: PloS One
https://www.readbyqxmd.com/read/28719287/phase-1-randomized-study-of-a-tetravalent-dengue-purified-inactivated-vaccine-in-healthy-adults-in-the-united-states
#3
Alexander C Schmidt, Leyi Lin, Luis J Martinez, Richard C Ruck, Kenneth H Eckels, Alix Collard, Rafael De La Barrera, Kristopher M Paolino, Jean-François Toussaint, Edith Lepine, Bruce L Innis, Richard G Jarman, Stephen J Thomas
AbstractThe safety and immunogenicity of four formulations of an investigational tetravalent dengue purified inactivated vaccine (DPIV), formulated at 1 or 4 μg with aluminum hydroxide (alum) or at 1 μg with an adjuvant system (AS01E or AS03B), were evaluated in a first-time-in-human, placebo-controlled, randomized, observer-blind, phase 1 trial in the continental United States. Two doses of vaccine or placebo were administered intramuscularly 4 weeks apart to 100 healthy adults 18-39 years of age, randomized 1:1:1:1:1 to receive one of four DPIV formulations or saline placebo...
June 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28719036/a-novel-method-for-achieving-optimal-neck-extension
#4
David Black, Thomas Snooks, Matthew Clark
From tonsillectomies to major neck dissections, a large proportion of ENT operations require the neck to be extended. This is normally achieved by a gel pad or sand bag shoulder bolster, or in more frugal hospitals - a bag of normal saline. In our theatres two sizes of shoulder bolster are generally available. This article is protected by copyright. All rights reserved.
July 18, 2017: Clinical Otolaryngology
https://www.readbyqxmd.com/read/28718886/brainstem-auditory-evoked-responses-in-37-dogs-with-otitis-media-before-and-after-topical-therapy
#5
S Paterson
OBJECTIVES: The objective of this study was to determine whether intra-aural administration of aqueous solutions of marbofloxacin, gentamicin, tobramycin and ticarcillin (used off-licence) was associated with changes in hearing as measured by brainstem auditory evoked responses. MATERIALS AND METHODS: Dogs diagnosed with otitis media (n=37) underwent brainstem auditory evoked response testing and then were treated for their ear disease. First, the external ear canal and middle ear were flushed with sterile saline followed by EDTA tris with 0·15% chlorhexidine...
July 18, 2017: Journal of Small Animal Practice
https://www.readbyqxmd.com/read/28718683/the-measurement-of-cough-response-to-bronchoconstriction-induced-by-methacholine-inhalation-in-healthy-subjects-an-examination-using-the-astograph-method
#6
Johsuke Hara, Masaki Fujimura, Noriyuki Ohkura, Tamami Sakai, Kenta Yamamura, Miki Abo, Hayato Koba, Satoshi Watanabe, Taro Yoneda, Shingo Nishikawa, Takashi Sone, Hideharu Kimura, Yoshihisa Ishiura, Kazuo Kasahara
BACKGROUND: We demonstrated that heightened cough response to bronchoconstriction is a fundamental feature of cough variant asthma (CVA). To evaluate this physiological feature of CVA in daily clinical practice, it is necessary to clarify the cough response to bronchoconstriction in healthy subjects. We evaluated cough response to methacholine (MCh)-induced bronchoconstriction in healthy subjects. A forced oscillometry technique was used to measure airway resistance changes with Mch. METHODS: Healthy never-smokers (21 men, 20 women; mean 22...
July 18, 2017: Experimental Lung Research
https://www.readbyqxmd.com/read/28718671/freeze-dried-and-irradiated-allograft-bone-combined-with-fresh-autologous-coagula-promotes-angiogenesis-in-an-ectopic-bone-allograft-implantation-model
#7
Haibo Lu, Haoye Meng, Shibi Lu, Ling Qin, Bin Zhao, Aiyuan Wang, Jiang Peng, Wenjing Xu, Quanyi Guo, Jian Zhang
BACKGROUND: Freeze-dried and irradiated allograft bone (FIAB) is more easily impacted than fresh-frozen allograft bone (FAB) but has weaker incorporation efficiency. We combined FIAB with fresh autologous coagula to enhance donor-host incorporation after impaction during hip revision. METHODS: Thirty adult male Sprague-Dawley rats were sacrificed for bone allograft harvesting, and nine male rats were subjected to ectopic bone allograft implantation. For each rat, the container on the left (study) side was filled with freeze-dried allograft bone powder and fresh autologous blood coagula, whereas the right (control) side was filled with freeze-dried allograft bone powder and physiological saline...
July 18, 2017: Connective Tissue Research
https://www.readbyqxmd.com/read/28718527/evaluation-of-intralesional-injection-of-hyaluronic-acid-compared-with-verapamil-in-peyronie-s-disease-preliminary-results-from-a-prospective-double-blinded-randomized-study
#8
V Favilla, G I Russo, A Zucchi, G Siracusa, S Privitera, S Cimino, M Madonia, T Cai, G Cavallini, G Liguori, G D'Achille, M Silvani, G Franco, P Verze, A Palmieri, B Torrisi, V Mirone, G Morgia
Several intralesional therapeutic protocols have been proposed for the treatment of Peyronie's disease. Among all, hyaluronic acid (HA) and verapamil have been differently tested. We aimed to evaluate the efficacy of intralesional verapamil (ILVI) compared with intralesional HA in patients with early onset of Peyronie's disease (PD). This is a multi-centre prospective double-arm, randomized, double-blinded study comparing ILVI vs. intralesional HA after 12-weeks. Sexually active men, older than 18 years and affected by the acute phase of PD were eligible for this study...
July 2017: Andrology
https://www.readbyqxmd.com/read/28718360/inflammatory-myopathy-in-horses-with-chronic-piroplasmosis
#9
Maria P Pasolini, Teresa B Pagano, Alessandro Costagliola, Davide De Biase, Barbara Lamagna, Luigi Auletta, Gerardo Fatone, Michele Greco, Pierpaolo Coluccia, Veneziano Veneziano, Claudio Pirozzi, Giuseppina Mattace Raso, Pasquale Santoro, Giuseppe Manna, Serenella Papparella, Orlando Paciello
Horses affected by chronic piroplasmosis may develop poor performance and muscle atrophy. Here we investigate the pathological and immunopathological aspects of myopathy occurring in chronic equine piroplasmosis. The study included 16 horses serologically positive for equine piroplasms presenting with clinical signs and supporting serum biochemical evidence of a myopathy. Skeletal muscle was evaluated by histopathology, immunohistochemistry, indirect immunofluorescence, and molecular detection of piroplasms and inflammatory cytokines in skeletal muscle...
January 1, 2017: Veterinary Pathology
https://www.readbyqxmd.com/read/28718218/impact-of-prenatal-immune-challenge-on-the-demyelination-injury-during-adulthood
#10
Abdeslam Mouihate, Hessah Al-Hashash, Sarah Rakhshani-Moghadam, Samah Kalakh
AIM: Brain inflammation is associated with several brain diseases such as multiple sclerosis (MS), a disease characterized by demyelination. Whether prenatal immune challenge affects demyelination-induced inflammation in the white matter during adulthood is unclear. In the present study, we used a well-established experimental model of focal demyelination to assess whether prenatal immune challenge affects demyelination-induced inflammation. METHODS: Pregnant rats were injected with either lipopolysaccharide (100 μg/kg, ip) or pyrogen-free saline...
July 17, 2017: CNS Neuroscience & Therapeutics
https://www.readbyqxmd.com/read/28718055/early-anti-inflammatory-and-pro-angiogenic-myocardial-effects-of-intravenous-serelaxin-infusion-for-72%C3%A2-h-in-an-experimental-rat-model-of-acute-myocardial-infarction
#11
Jesus Sanchez-Mas, Antonio Lax, Mari C Asensio-Lopez, Miriam Lencina, Maria J Fernandez-Del Palacio, Angela Soriano-Filiu, Rudolf A de Boer, Domingo A Pascual-Figal
Sprague Dawley rats were subjected to acute myocardial infarction (AMI) by permanent ligation of the left anterior descending coronary artery. At the time of AMI, a subcutaneous mini-osmotic pump was implanted and animals were randomized into three groups, according to the intravenous therapy received during the first 72 h: placebo-treated (saline), serelaxin10-treated (SRLX10 = 10 μg/kg/day), or serelaxin30-treated (SRLX30 = 30 μg/kg/day). Treatment with SRLX30 reduced the expression of inflammatory cytokines and chemokines, as well as the infiltration of macrophages, and increased the expression of pro-angiogenic markers and vessel density in the infarcted myocardium after 7 days...
July 17, 2017: Journal of Cardiovascular Translational Research
https://www.readbyqxmd.com/read/28718012/implant-decontamination-with-phosphoric-acid-during-surgical-peri-implantitis-treatment-a-rct
#12
Diederik F M Hentenaar, Yvonne C M De Waal, Hans Strooker, Henny J A Meijer, Arie-Jan Van Winkelhoff, Gerry M Raghoebar
BACKGROUND: Peri-implantitis is known as an infectious disease that affects the peri-implant soft and hard tissue. Today, scientific literature provides very little evidence for an effective intervention protocol for treatment of peri-implantitis. The aim of the present randomized controlled trial is to evaluate the microbiological and clinical effectiveness of phosphoric acid as a decontaminating agent of the implant surface during surgical peri-implantitis treatment. METHODS: Peri-implantitis lesions were treated with resective surgical treatment aimed at peri-implant granulation tissue removal, bone recontouring, and pocket elimination...
December 2017: International Journal of Implant Dentistry
https://www.readbyqxmd.com/read/28718009/relative-salinity-tolerance-of-rice-cultivars-native-to-north-east-india-a-physiological-biochemical-and-molecular-perspective
#13
Takhellambam Omisun, Smita Sahoo, Bedabrata Saha, Sanjib Kumar Panda
Salinity is the second most prevalent abiotic stress faced by plants, and rice is not an exception. Through this study, it has been tried upon, to study the relative salinity tolerance of eight local varieties of North East India. Preliminary screening was based on their dose- and time-dependent physiological responses to salinity stress. Among the cultivars, Tampha was found to be relatively more tolerant, whereas MSE9 the most sensitive. To further ascertain their tolerance capacity, MDA and H2O2 content was determined, which also confirmed the tolerance level of the two cultivars...
July 17, 2017: Protoplasma
https://www.readbyqxmd.com/read/28717995/ovomucin-nanoparticles-promising-carriers-for-mucosal-delivery-of-drugs-and-bioactive-compounds
#14
Ali Akbari, Jianping Wu
Ovomucin, with a similar structure to mucin and gelation ability at room temperature, is a potential mucosal carrier. To evaluate this potential, the mucoadhesive property of ovomucin was studied by the tensile strength and rheology methods, and compared with three known mucoadhesive polymers, chitosan, polyacrylic acid (PAA), and alginate. Ovomucin particles were basically spherical in shape with an average size of ~572, 235 and 54 nm prepared at pH 5, 6.5, and 9, respectively. The absolute value of the zeta potential increased from ~ -51 mV at pH 5 to ~ -59 and -91 mV at pH 6...
July 17, 2017: Drug Delivery and Translational Research
https://www.readbyqxmd.com/read/28717650/removal-of-cu-zn-pb-and-cr-from-yangtze-estuary-using-the-phragmites-australis-artificial-floating-wetlands
#15
Xiaofeng Huang, Feng Zhao, Gao Yu, Chao Song, Zhi Geng, Ping Zhuang
Contamination of heavy metals would threaten the water and soil resources; phytoremediation can be potentially used to remediate metal contaminated sites. We constructed the Phragmites australis artificial floating wetlands outside the Qingcaosha Reservoir in the Yangtze Estuary. Water characteristic variables were measured in situ by using YSI Professional Pro Meter. Four heavy metals (copper, zinc, lead, and chromium) in both water and plant tissues were determined. Four heavy metals in estuary water were as follows: 0...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28717597/cuticle-hydrocarbons-in-saline-aquatic-beetles
#16
María Botella-Cruz, Adrián Villastrigo, Susana Pallarés, Elena López-Gallego, Andrés Millán, Josefa Velasco
Hydrocarbons are the principal component of insect cuticle and play an important role in maintaining water balance. Cuticular impermeability could be an adaptative response to salinity and desiccation in aquatic insects; however, cuticular hydrocarbons have been poorly explored in this group and there are no previous data on saline species. We characterized cuticular hydrocarbons of adults and larvae of two saline aquatic beetles, namely Nebrioporus baeticus (Dytiscidae) and Enochrus jesusarribasi (Hydrophilidae), using a gas chromatograph coupled to a mass spectrometer...
2017: PeerJ
https://www.readbyqxmd.com/read/28717536/safety-and-efficacy-of-bipolar-energy-for-transurethral-resection-of-bladder-tumours-a-prospective-quasi-randomized-study
#17
Vasudevan Thirugnanasambandam, Jeyaraman Ramanathan
OBJECTIVE: The primary aim of the study was to evaluate the safety and efficacy of bipolar energy in resecting bladder tumors. MATERIAL AND METHODS: The study was done between March 2012 and February 2013. This is a prospective quasi-randomized study evaluating the safety and efficacy of bipolar energy in transurethral resection of bladder tumours (TURBT). Institutional Ethics Committee approval was obtained. Informed consent was taken from all patients. Patients were randomized into two groups of 50 each to undergo either monopolar TURBT or bipolar TURBT using glycine and saline irrigation solutions, respectively...
June 2017: Turkish Journal of Urology
https://www.readbyqxmd.com/read/28717520/modeling-chronic-dacryocystitis-in-rabbits-by-nasolacrimal-duct-obstruction-with-self-curing-resin
#18
Kai Hou, Tao Ai, Rong Liu, Nan Xiang, Jing Jin, Weikun Hu, Ban Luo
We established a chronic dacryocystitis model by injecting of 0.05, 0.1, and 0.15 ml self-curing resin via the lacrimal punctum in rabbits. Animals were randomized into four groups (n = 11 animals/group). The control group received 0.15 ml normal saline. Within three months postinjection, epiphora and eye discharge were observed. At the 90th day postlacrimal passage irrigation, CT dacryocystography was performed to find changes in the lacrimal image, and hematoxylin and eosin staining was made to identify pathological changes of the lacrimal sac...
2017: Journal of Ophthalmology
https://www.readbyqxmd.com/read/28717360/ethanol-enhances-high-salinity-stress-tolerance-by-detoxifying-reactive-oxygen-species-in-arabidopsis-thaliana-and-rice
#19
Huong Mai Nguyen, Kaori Sako, Akihiro Matsui, Yuya Suzuki, Mohammad Golam Mostofa, Chien Van Ha, Maho Tanaka, Lam-Son Phan Tran, Yoshiki Habu, Motoaki Seki
High-salinity stress considerably affects plant growth and crop yield. Thus, developing techniques to enhance high-salinity stress tolerance in plants is important. In this study, we revealed that ethanol enhances high-salinity stress tolerance in Arabidopsis thaliana and rice. To elucidate the molecular mechanism underlying the ethanol-induced tolerance, we performed microarray analyses using A. thaliana seedlings. Our data indicated that the expression levels of 1,323 and 1,293 genes were upregulated by ethanol in the presence and absence of NaCl, respectively...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28717318/hemogram-responses-in-goats-toward-challenged-with-corynebacterium-pseudotuberculosis-and-its-immunogen-mycolic-acids
#20
Mohammed Naji Odhah, Faez Firdaus Jesse Abdullah, Abd Wahid Haron, Mohd Azmi Mohd Lila, Mohd Zamri-Saad, Zaid Khuder, Idris Umar Hambali, Muhammed Umar, Wessam Monther Saleh
AIM: This study was conducted to analyze the changes in blood profile of goats inoculated with Corynebacterium pseudotuberculosis and its immunogen mycolic acid (MA) extract. MATERIALS AND METHODS: A total of 12 clinically healthy crossbred Boer female goats were divided into three groups; A, B and C (4 goats each per group). Group A was inoculated with 2 ml sterile phosphate buffered saline via intradermal route as the negative control group whilst Group B was inoculated with 2 ml of MA extract (1 g/ml) intradermally and Group C was then inoculated with 2 ml (1×10(9)) colony forming unit of active C...
June 2017: Veterinary World
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