Bairen Pang, Qi Wang, Haotian Chen, Zhihan Liu, Meng Han, Jie Gong, Liang Yue, Xuan Ding, Suying Wang, Zejun Yan, Yingzhi Chen, David Malouf, Joseph Bucci, Tiannan Guo, Cheng Zhou, Junhui Jiang, Yong Li
Diagnosis and stratification of prostate cancer (PCa) patients using the prostate-specific antigen (PSA) test is challenging. Extracellular vesicles (EVs), as a new star of liquid biopsy, has attracted interest to complement inaccurate PSA screening and invasiveness of tissue biopsy. In this study, a panel of potential small EV (sEV) protein biomarkers is identified from PCa cell lines using label-free LC-MS/MS proteomics. These biomarkers underwent further validation with plasma and urine samples from different PCa stages through parallel reaction monitoring-based targeted proteomics, western blotting, and ELISA...
April 8, 2024: Advanced Science (Weinheim, Baden-Wurttemberg, Germany)