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https://www.readbyqxmd.com/read/29166623/anticancer-potential-of-zno-nanoparticle-ferulic-acid-conjugate-on-huh-7-and-hepg2-cells-and-diethyl-nitrosamine-induced-hepatocellular-cancer-on-wistar-albino-rat
#1
E Preedia Babu, A Subastri, A Suyavaran, S Nithyananthan, M Suresh Kumar, K Premkumar, R Bharathidasan, C Thirunavukkarasu
Drawbacks and limitations of recently available therapies to hepatocellular cancer (HCC) devoted the scientist to focus on emerging new strategies. ZnO nanoparticles (ZnONPs) based chemotherapeutics has been emanating as a promising approach to maximize therapeutic synergy facilitating the discovery of novel multitargeted combinations. In the present study we conjugated ZnONPs with ferulic acid (ZnONPs-FAC) characterized by computational, spectroscopic and microscopic techniques. In vitro anticancer potential has been evaluated by assessing cell viability, morphology, ROS generation, mitochondrial membrane permeability, comet assay, immunofluorescent staining of 8-OHdG, Ki67 and γ-H2AX, cell cycle analysis and western blot analysis and in vivo anticancer potential against DEN induced HCC was analyzed by histopathological and immunohistochemical methods...
November 19, 2017: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/29166598/biological-significance-of-the-suppression-of-oxidative-phosphorylation-in-induced-pluripotent-stem-cells
#2
Cheng Zhang, Maria Skamagki, Zhong Liu, Aparna Ananthanarayanan, Rui Zhao, Hu Li, Kitai Kim
We discovered that induced pluripotent stem cell (iPSC) clones generated from aged tissue donors (A-iPSCs) fail to suppress oxidative phosphorylation. Compared to embryonic stem cells (ESCs) and iPSCs generated from young donors (Y-iPSCs), A-iPSCs show poor expression of the pluripotent stem cell-specific glucose transporter 3 (GLUT3) and impaired glucose uptake, making them unable to support the high glucose demands of glycolysis. Persistent oxidative phosphorylation in A-iPSCs generates higher levels of reactive oxygen species (ROS), which leads to excessive elevation of glutathione (a ROS-scavenging metabolite) and a blunted DNA damage response...
November 21, 2017: Cell Reports
https://www.readbyqxmd.com/read/29166584/swell-or-not-too-swell-cytokines-regulate-arterial-aneurysm-formation
#3
Peter Libby, Amélie Vrooman
Arterial remodeling participates pivotally in many diseases including arterial aneurysms. In this issue of Immunity, Da Ros et al. (2017) report that, in experimental aortic aneurysm formation, neutralization of interleukin-1β reduced arterial wall stiffness and hampered aneurysm development.
November 21, 2017: Immunity
https://www.readbyqxmd.com/read/29166568/camel-whey-protein-protects-lymphocytes-from-apoptosis-via-the-pi3k-akt-nf-%C3%AE%C2%BAb-atf-3-and-hsp-70-signaling-pathways-in-heat-stressed-male-mice
#4
Gamal Badr, Nancy K Ramadan, Hanem S Abdel-Tawab, Samia F Ahmed, Mohamed H Mahmoud
Heat stress (HS) is an environmental factor that depresses the immune systems mediating dysfunctional immune cells. Camel whey protein (CWP) can scavenge free radicals and enhance immunity. The present study investigated the impact of dietary supplementation with CWP on immune dysfunction induced by exposure to HS. Male mice (n = 45) were divided into three groups: control group; HS group; and HS mice that were orally administered CWP (HS+CWP group). The HS group exhibited elevated levels of reactive oxygen species (ROS) and pro-inflammatory cytokines (IL-1β, IL-6, TNF-α) as well as a significant reduction in the IL-2 and IL-4 levels...
November 22, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/29165875/4%C3%AE-hydroxywithanolide-e-selectively-induces-oxidative-dna-damage-for-selective-killing-of-oral-cancer-cells
#5
Jen-Yang Tang, Hurng-Wern Huang, Hui-Ru Wang, Ya-Ching Chan, Jo-Wen Haung, Chih-Wen Shu, Yang-Chang Wu, Hsueh-Wei Chang
Reactive oxygen species (ROS) induction had been previously reported in 4β-hydroxywithanolide (4βHWE)-induced selective killing of oral cancer cells, but the mechanism involving ROS and the DNA damage effect remain unclear. This study explores the role of ROS and oxidative DNA damage of 4βHWE in the selective killing of oral cancer cells. Changes in cell viability, morphology, ROS, DNA double strand break (DSB) signaling (γH2AX foci in immunofluorescence and DSB signaling in western blotting), and oxidative DNA damage (8-oxo-2'deoxyguanosine [8-oxodG]) were detected in 4βHWE-treated oral cancer (Ca9-22) and/or normal (HGF-1) cells...
November 22, 2017: Environmental Toxicology
https://www.readbyqxmd.com/read/29165873/andrographolide-inhibits-hypoxia-induced-hypoxia-inducible-factor-1%C3%AE-and-endothelin-1-expression-through-the-heme-oxygenase-1-co-cgmp-mkp-5-pathways-in-ea-hy926-cells
#6
Hung-Chih Lin, Shih-Li Su, Wan-Chun Lin, Ai-Hsuan Lin, Ya-Chen Yang, Chong-Kuei Lii, Haw-Wen Chen
Andrographolide is a potent anti-inflammatory agent found in Andrographis paniculata. Endothelin 1 (ET-1) is an endothelium-derived vasoconstrictor with pro-inflammatory properties secreted in response to hypoxia. Mitogen-activated protein kinase phosphatase 5 (MKP-5) is a dual-specificity phosphatase that dephosphorylates threonine and tyrosine residues of MAPKs. We showed previously that hypoxia-induced HIF-1α expression and ET-1 secretion are dependent on p38 MAPK in EA.hy926 cells. Here, we investigate what role MKP-5 plays in andrographolide's inhibition of hypoxia-induced expression of HIF-1α and ET-1...
November 22, 2017: Environmental Toxicology
https://www.readbyqxmd.com/read/29165856/saccharomyces-cerevisiae-hog1-map-kinase-pathway-is-activated-in-response-to-honokiol-exposure
#7
Lingmei Sun, Kai Liao
AIM: The goal of the study was to investigate the cellular tolerance mechanism in response to honokiol exposure. METHODS AND RESULTS: The broth microdilution method was employed to test the sensitivity of different Saccharomyces cerevisiae strains to honokiol. Intracellular levels of reactive oxygen species (ROS) were determined by DCFH-DA staining. The phosphorylation of Hog1 was evaluated by western blot analysis. The mRNA expressions of genes involved in the Ras-cyclic AMP (cAMP) pathway were analyzed by real-time reverse transcription polymerase chain reaction...
November 22, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29165597/gsta1-expression-is-correlated-with-aldosterone-level-in-kcnj5-mutated-adrenal-aldosterone-producing-adenoma
#8
Xintao Li, Baojun Wang, Lu Tang, Yu Zhang, Luyao Chen, Liangyou Gu, Fan Zhang, Jinzhi Ouyang, Xu Zhang
Context: KCNJ5 mutation is a major cause of aldosterone-producing adenoma (APA). The development of APA apart from KCNJ5 mutation is less investigated. Objective: To investigate other mechanisms affecting aldosterone secretion apart from KCNJ5. Patients and Methods: Six pairs of KCNJ5-mutated, high and low aldosterone-secreting APAs, five non-KCNJ5-mutated APAs and four normal adrenal glands were assayed by Affymetrix Genechip Human Transcriptome Array 2...
November 20, 2017: Journal of Clinical Endocrinology and Metabolism
https://www.readbyqxmd.com/read/29165474/sugar-and-mineral-enriched-fraction-from-olive-mill-wastewater-for-promising-cosmeceutical-application-characterization-in-vitro-and-in-vivo-studies
#9
Maria Domenica Di Mauro, Barbara Tomasello, Roberta Carla Giardina, Sandro Dattilo, Veronica Mazzei, Fulvia Sinatra, Massimo Caruso, Nicola D'Antona, Marcella Renis
Nowadays, agro-food by-products represent a potential low-cost source of biologically active ingredients which have been paid significant attention as nutraceuticals, medicine, food and cosmetics. In a previous study we evaluated the total sugars, metals and polyphenols of olive mill wastewater (OMWW) from a Cerasuola olive cultivar. In the present work we selectively recovered a sugar and mineral enriched fraction (SMEF) from Cerasuola OMWW by a green adsorption/desorption process. The SMEF was mainly found to be composed of monosaccharides and potassium by HPLC-ELSD and ICP-MS...
November 22, 2017: Food & Function
https://www.readbyqxmd.com/read/29165383/a-mini-review-of-the-nadph-oxidases-in-vascular-dementia-correlation-with-noxs-and-risk-factors-for-vad
#10
REVIEW
Dong-Hee Choi, Jongmin Lee
Oxidative stress (OS) is one of the factors that cause dementia conditions such as Alzheimer's disease and vascular dementia (VaD). In the pathogenesis of VaD, OS is associated with risk factors that include increased age, hypertension, and stroke. Nicotinamide adenine dinucleotide phosphate (NADPH) oxidases (NOXs) are a molecular source of reactive oxygen species (ROS). According to recent studies, inhibition of NOX activity can reduce cognitive impairment in animal models of VaD. In this article, we review the evidence linking cognitive impairment with NOX-dependent OS, including the vascular NOX and non-vascular NOX systems, in VaD...
November 22, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29165364/the-rosiglitazone-like-effects-of-vitexilactone-a-constituent-from-vitex-trifolia-l-in-3t3-l1-preadipocytes
#11
Atsuyoshi Nishina, Masaya Itagaki, Daisuke Sato, Hirokazu Kimura, Yasuaki Hirai, Nyunt Phay, Makoto Makishima
The increased number of patients with type 2 diabetes (T2D) has become a worldwide problem, and insulin sensitizers such as thiazolidinediones (TZDs) are used as therapeutic agents. We found that extracts of Vitex trifolia L. (V. trifolia), a medicinal plant from Myanmar, induced adipogenesis similar to rosiglitazone (ROS), which is a TZD, in 3T3-L1 preadipocytes. In the present study, we attempted to isolate from V. trifolia those compounds that showed ROS-like effects. Among the extracts of hexane, ethyl acetate, and methanol obtained from V...
November 22, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29165163/the-potential-protective-effect-of-commelina-nudiflora-l-against-carbon-tetrachloride-ccl4-induced-hepatotoxicity-in-rats-mediated-by-suppression-of-oxidative-stress-and-inflammation
#12
Muhammad Dawood Shah, Urban J A D'Souza, Mohammad Iqbal
BACKGROUND: This study aims to assess the hepatoprotective potential of Commelina nudiflora against CCl4-induced hepatic injury in rats. METHOD: Antioxidant activities were determined. Phytochemical analysis was performed by gas chromatography mass spectrometry (GCMS). In the in vivo study, Sprague Dawley rats were pretreated with C. nudiflora (150, 300, and 450 mg kg body weight (b.wt.)) once daily for 14 days followed by two doses of CCl4 (1 ml/kg b.wt.). After 2 weeks, the rats were sacrificed and hepatoprotective analysis was performed...
September 11, 2017: Environmental Health and Preventive Medicine
https://www.readbyqxmd.com/read/29165097/a-predictive-model-of-the-prevalence-of-delirium-in-elderly-subjects-admitted-to-nursing-homes
#13
Pilar Perez-Ros, Francisco Miguel Martinez-Arnau, Susana Baixauli-Alacreu, Jose Fermin Garcia-Gollarte, Francisco Tarazona-Santabalbina
INTRODUCTION: Delirium is common in geriatric patients admitted to nursing homes, with an incidence of 22-79% among long-term residents. AIM: To establish a predictive model of the risk of delirium episodes in a sample of elderly people living in nursing homes. MATERIAL AND METHODS: A retrospective, cross-sectional case-control study covering a period of 12 consecutive months (April 2014 - March 2015) was carried out. The included cases had suffered at least one episode of delirium during the study period...
November 20, 2017: Endocrine, Metabolic & Immune Disorders Drug Targets
https://www.readbyqxmd.com/read/29165056/citrinin-induces-apoptosis-in-human-hct116-colon-cancer-cells-through-endoplasmic-reticulum-stress
#14
Amal Salah, Chayma Bouaziz, Alexandre Prola, Julie Pires Da Silva, Hassen Bacha, Salwa Abid-Essefi, Christophe Lemaire
The mycotoxin citrinin (CTN) is a natural contaminant of various human foods that may produce serious adverse health problems. Several studies demonstrated that citrinin exerts cytotoxic and genotoxic effects in both in vivo and in vitro systems. However, the precise mechanisms of action (MOA), particularly in intestinal cells remain unclear. The aim of the present study was to examine the precise MOA of citrinin in vitro. Data demonstrated that CTN significantly decreased the number of viable human intestinal HCT116 cells and induced apoptotic events including (1) decrease in ΔѰm indicative of mitochondrial membrane permeabilization, (2) activation of caspase 3, (3) elevated production of reactive oxygen species (ROS) and (4) relative persistence of plasma membrane integrity...
2017: Journal of Toxicology and Environmental Health. Part A
https://www.readbyqxmd.com/read/29165041/akt-mediated-phosphorylation-of-atg4b-impairs-mitochondrial-activity-and-enhances-the-warburg-effect-in-hepatocellular-carcinoma-cells
#15
Zhenhong Ni, Jintao He, Yaran Wu, Changjiang Hu, Xufang Dai, Xiaojing Yan, Bo Li, Xinzhe Li, Haojun Xiong, Yuming Li, Song Li, Liang Xu, Yongsheng Li, Jiqin Lian, Fengtian He
Phosphorylation is a major type of post-translational modification, which can influence the cellular physiological function. ATG4B, a key macroautophagy/autophagy-related protein, has a potential effect on the survival of tumor cells. However, it is still unknown as to the role of ATG4B phosphorylation in cancers. In this study, we identified a novel phosphorylation site at Ser34 of ATG4B induced by AKT in HCC cells. The phosphorylation of ATG4B at Ser34 had little effect on autophagic flux, but promoted the Warburg effect including the increase of L-lactate production and glucose consumption, and the decrease of oxygen consumption in HCC cells...
November 22, 2017: Autophagy
https://www.readbyqxmd.com/read/29164887/pterostilbene-enhances-trail-induced-apoptosis-through-the-induction-of-death-receptors-and-downregulation-of-cell-survival-proteins-in-trail-resistance-triple-negative-breast-cancer-cells
#16
Chao-Ming Hung, Liang-Chih Liu, Chi-Tang Ho, Ying-Chao Lin, Tzong-Der Way
Tumor necrosis factor-related apoptosis-induced ligand (TRAIL) is nontoxic to normal cells and preferentially cytotoxic to cancer cells. However, recent data suggest that malignant breast cancer cells tend to be resistant to TRAIL. Pterostilbene (PTER), a natural dimethylated analog of resveratrol, is known to have diverse pharmacologic activities, including anticancer properties. In the present study, we investigated whether PTER affects TRAIL-induced apoptosis and its mechanism in TRAIL-resistant triple negative breast cancer (TNBC) cells...
November 22, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29164653/gradient-sperm-selection-for-reproductive-techniques-in-cattle-is-isolate-a-suitable-replacement-for-percoll
#17
B Sepúlveda, M E Arias, L Aguila, F Zambrano, R Sánchez, R Felmer
In assisted reproductive techniques, it is essential to perform a sperm selection to obtain spermatozoa with high motility and membrane integrity for in vitro fertilisation (IVF) and high-DNA integrity for intracytoplasmic sperm injection (ICSI). In this study, we evaluated whether Isolate(®) was a suitable substitute for Percoll(®) for assisted reproductive techniques. Commercial cryopreserved bovine semen was used after selection in both gradients, and plasma and acrosome membrane integrity, reactive oxygen species (ROS) levels, DNA integrity and mitochondrial membrane potential (ΔΨm) were assessed by flow cytometry...
November 21, 2017: Andrologia
https://www.readbyqxmd.com/read/29164624/suppression-of-uvb-mediated-matrix-metalloproteinase-generation-by-sorbus-commixta-twig-extract-in-human-dermal-fibroblasts
#18
Song Hua Xuan, Young Min Park, So Hyun Park, Hyo Jin Jeong, Soo Nam Park
Sorbus commixta is a traditional oriental medicinal plant that grows in East Asian countries such as Korea, Japan, and China. The twig of S. commixta has been considered valuable for centuries to treat diseases including asthma, cough, and other bronchial disorders. However, the effect of S. commixta twig extract on human skin has not been investigated well. The present study aimed at assessing the anti-photoaging effect of S. commixta twig ethanol extract (STE) on ultraviolet B (UVB)-induced matrix metalloproteinase (MMP) levels and its underlying mechanism in human dermal fibroblasts...
November 22, 2017: Photochemistry and Photobiology
https://www.readbyqxmd.com/read/29164589/mir-214-protects-mc3t3-e1-osteoblasts-against-h2o2-induced-apoptosis-by-suppressing-oxidative-stress-and-targeting-atf4
#19
X-Z Lu, Z-H Yang, H-J Zhang, L-L Zhu, X-L Mao, Y Yuan
OBJECTIVE: Fragility fracture is one of the common complications of osteoporosis. Elevated oxidative stress-induced apoptosis is thought to be one of the unfavorable factors to osteoblastic dysfunction, which increased the risk of bone fracture. However, the molecular mechanisms for oxidative stress-induced osteoblast cells apoptosis still need to be elucidated. This study aims to investigate the protective function of miR-214 in H2O2-induced apoptosis of MC3T3-E1 osteoblasts. MATERIALS AND METHODS: MC3T3-E1 cells were treated with 400 μM H2O2...
November 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29164403/a-novel-photodynamic-therapy-based-drug-delivery-system-layered-on-a-stent-for-treating-cholangiocarcinoma
#20
Po-Chin Liang, Kai-Wen Huang, Chien-Chih Tung, Ming-Chu Chang, Fuh-Yu Chang, Jau-Min Wong, Yu-Ting Chang
This study aimed to investigate the drug delivery efficacy and bio-effectiveness of a novel photodynamic therapy (PDT)-matrix drug delivery system for cholangiocarcinoma (CCA). Metallic stents were coated with polyurethane (PU) as the first layer. A 2-hydroxyethyl methacrylate (2-HEMA)/ethylene glycol dimethacrylate (EGDMA)/benzoyl peroxide (BPO) layer and a poly(ethylene-co-vinyl acetate) (PEVA)/poly(n-butyl methacrylate) (PBMA)/polyvinylpyrrolidone K30 (K30) layer containing various concentrations of Photofrin were then incorporated onto the stent as the second and third layers...
November 22, 2017: Biomedical Microdevices
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