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Lu-Lu Yin, Hong Yuan, Ke-Jie Du, Bo He, Shu-Qin Gao, Ge-Bo Wen, Xiangshi Tan, Ying-Wu Lin
A de novo designed intramolecular disulfide bond in myoglobin, resembling that in cytoglobin without structural evidence, was confirmed by an X-ray structure for the first time and was demonstrated to regulate both the structure and function of this protein, which fulfills the design of an artificial dehaloperoxidase, with an activity exceeding that of a native enzyme.
April 12, 2018: Chemical Communications: Chem Comm
Alberto Leonardi, Laura Penta, Marta Cofini, Lucia Lanciotti, Nicola Principi, Susanna Esposito
Background: Autoimmune hypothyroidism (Hashimoto thyroiditis; HT) is the most common postnatal thyroid disease. Clinical manifestations of HT vary according to disease severity. Due to the pleiotropic effects of thyroid hormone, less common signs and symptoms of HT can occur, leading to a delay in diagnosis. Case presentation: A 9-year-old girl of Indian origin was admitted for a one-week history of widespread myalgia, fatigue, muscle weakness, difficulty walking, and a significant increase in weight (approximately 2 kg) without any changes in daily habits...
April 9, 2018: International Journal of Environmental Research and Public Health
Yohko F Yano, Etsuo Arakawa, Wolfgang Voegeli, Chika Kamezawa, Tadashi Matsushita
We present the results of time-resolved X-ray reflectivity measurements carried out to investigate the early stage of protein adsorption and deformation at an air-water interface. Three globular proteins (lysozyme, myoglobin, and BSA) were studied, and we observed that the proteins adsorbed at the air-water interface initially possessed a thinner conformation than their native structures. The degree of deformation increased in the order myoglobin < lysozyme < BSA, which was inconsistent with the order of molecular flexibility...
April 9, 2018: Journal of Physical Chemistry. B
Young Shin Lim, Heeyeon Cho, Sang Taek Lee, Yeonhee Lee
Purpose: This study aimed to evaluate the clinical findings in pediatric rhabdomyolysis and the predictive factors for acute kidney injury (AKI) in Korean children. Methods: Medical records of 39 Korean children, who were newly diagnosed with rhabdomyolysis from January 2008 to December 2015, were retrospectively analyzed. The diagnosis was made from the medical history, elevated serum creatinine kinase level >1,000 IU/L, and plasma myoglobin level >150 ng/mL...
March 2018: Korean Journal of Pediatrics
Shu-Ichi Nakano, Daisuke Yamaguchi, Naoki Sugimoto
Spatially confined environments are seen in biological systems and in the fields of biotechnology and nanotechnology. The confinement restricts the conformational space of polymeric molecules and increasing the degree of molecular crowding. Here, we developed preparation methods for agarose and polyacrylamide gels applicable to UV spectroscopy that can evaluate the confinement effects on DNA and protein structures. Measurements of UV absorbance and CD spectra showed no significant effect of the confinement in the porous media of agarose gels on the base-pair stability of DNA polynucleotides [poly(dA)/poly(dT)] and oligonucleotides (hairpin, duplex, and triplex structures)...
April 6, 2018: Molecular Biology Reports
Yue Ying, Yue-Kai Hu, Jia-Lin Jin, Ji-Ming Zhang, Wen-Hong Zhang, Yu-Xian Huang
BACKGROUND: Current treatment options for chronic hepatitis B (CHB) are pegylated interferon alpha and nucleoside analogues (NAs). NAs have relatively fewer side effects than interferon alpha, and generally well tolerated. Previously 12.9% of patients on telbivudine treatment were reported to develop severe elevation of serum creatine phosphokinase (CPK) levels, but related clinical disease, like lactic acidosis (LA) and rhabdomyolysis (RM) were rare. The pathophysiology may be mitochondrial toxicity, for the NAs inhibit not only hepatitis B virus (HBV) polymerase, but also the host mitochondrial DNA polymerase γ...
April 6, 2018: BMC Gastroenterology
William S Kish, Matthew K Roach, Hiroyuki Sachi, Amith D Naik, Stefano Menegatti, Ruben G Carbonell
Prior work described the identification and characterization of erythropoietin-binding cyclic peptides SLFFLH, VVFFVH, FSLLHH and FSLLSH (all of the form cyclo[(Nα -Ac)Dap(A)-X1 -X6 -AE], wherein X1 -X6 is the listed sequences). In this work, the peptide ligands were synthesized on Toyopearl chromatographic resins and utilized for purifying recombinant human erythropoietin (rHuEPO) from complex sources. Elution buffer pH and composition were optimized to maximize the recovery of standard rHuEPO from the peptide resins...
March 27, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Aurélie Servonnet, Clément Dubost, Guillaume Martin, Bertrand Lefrère, Eléonore Fontan, Franck Ceppa, Hervé Delacour
The clinical biologist plays a role as a consultant for the relevant use of biological examination. Advisory activities of the medical laboratory may help physician in diagnosis or therapeutic algorithm, avoiding redundant ordering or useless tests. In this context, we performed a review of literature about the clinically interest of myoglobin assays. The indications of myoglobin's assays appear fairly limited. It is no longer mentioned in the European guidelines for the management of acute coronary syndromes in patients presenting without persistent ST-segment elevation...
April 1, 2018: Annales de Biologie Clinique
Johannes Schmid, Laura Liesinger, Ruth Birner-Gruenberger, Tatjana Stojakovic, Hubert Scharnagl, Benjamin Dieplinger, Martin Asslaber, Roman Radl, Meinrad Beer, Malgorzata Polacin, Johannes Mair, Dieter Szolar, Andrea Berghold, Stefan Quasthoff, Josepha S Binder, Peter P Rainer
BACKGROUND: Cardiac troponins are often elevated in patients with skeletal muscle disease who have no evidence of cardiac disease. OBJECTIVES: The goal of this study was to characterize cardiac troponin concentrations in patients with myopathies and derive insights regarding the source of elevated troponin T measurements. METHODS: Cardiac troponin T (cTnT) and cardiac troponin I (cTnI) concentrations were determined by using high sensitivity assays in 74 patients with hereditary and acquired skeletal myopathies...
April 10, 2018: Journal of the American College of Cardiology
Vladimir Manojlović, Frane Erčulj
BACKGROUND: It is well known that eccentric muscle contractions induce delayed onset muscle soreness (DOMS) and exertional rhabdomyolysis (ERB), both of which are related to high blood levels of muscle damage markers after exercise. Creatine kinase (CK) is, together with some other markers (e.i. myoglobin, α-actin, skeletal troponin), widely used in determination of muscle damage. Moreover, DOMS after eccentric exercise has been shown to be associated with altered blood lactate concentration after subsequent submaximal or maximal cycling exercise...
April 4, 2018: Journal of Sports Medicine and Physical Fitness
G B Postnikova, E A Shekhovtsova
In this review, we shortly summarize the data of our studies (and also corresponding studies of other authors) on the new mechanism of myoglobin (Mb) deoxygenation in a cell, according to which Mb acts as an oxygen transporter, and its affinity for the ligand, like in other transporting proteins, is regulated by the interaction with the target, in our case, mitochondria (Mch). We firstly found that contrary to previously formulated and commonly accepted concepts, oxymyoglobin (MbO2 ) deoxygenation occurs only via interaction of the protein with respiring mitochondria (low pO2 values are necessary but not sufficient for this process to proceed)...
February 2018: Biochemistry. Biokhimii︠a︡
Parisa Farzam, Zack Starkweather, Maria A Franceschini
There is a growing interest in monitoring muscle oxygen saturation (SmO2 ), which is a localized measure of muscle oxidative metabolism and can be acquired continuously and noninvasively using near-infrared spectroscopy (NIRS) methods. Most NIRS systems are cumbersome, expensive, fiber coupled devices, with use limited to lab settings. A novel, low cost, wireless, wearable has been developed for use in athletic training. In this study, we evaluate the advantages and limitations of this new simple continuous-wave (CW) NIRS device with respect to a benchtop, frequency-domain near-infrared spectroscopy (FDNIRS) system...
April 2018: Physiological Reports
James D Sanders, Dmitry Grinfeld, Konstantin Aizikov, Alexander A Makarov, Dustin D Holden, Jennifer S Brodbelt
We demonstrate a method for determining the collision cross sections (CCS) of protein ions based on the decay rate of the time domain transient signal from an OrbitrapTM mass analyzer. Multiply charged ions of ubiquitin, cytochrome c and myoglobin were generated by electrospray ionization of both denaturing solutions and ones with high salt content to preserve native-like structures. A linear relationship between the pressure in the Orbitrap analyzer and the transient decay rate was established and used to demonstrate that the signal decay is primarily due to ion-neutral collisions for protein ions across the entire working pressure range of the instrument...
April 2, 2018: Analytical Chemistry
Soraya de Chadarevian
Quite exactly 60 years ago, an article in Nature presented the first low-resolution model of a globular protein derived by direct structure determination. The work was part of an ongoing effort by John Kendrew and his colleagues at the Medical Research Council Unit for Molecular Biology in Cambridge (UK) that two years later led to the publication of the first atomic structure of the same protein, myoglobin.1,2 In 1962, John Kendrew together with Max Perutz, working in the same laboratory, received the Nobel Prize for Chemistry for their studies of the structures of globular proteins...
April 1, 2018: Protein Science: a Publication of the Protein Society
Simone M Gonçalves, Karina B Gomes, Maria das G Carvalho, Henrique P B Magalhães, Edna A Reis, Iêda de Fátima O Silva
BACKGROUND: Among cardiovascular diseases (CVD), acute coronary syndrome (ACS) is the main manifestation, corresponding to signs and symptoms that occur with ischemia and outcome of angina or acute myocardial infarction (AMI). The aim of this study was to investigate the performance of biochemical markers eligible in a chest pain protocol, using Point of care Test (POCT), in patients in a reference emergency room. METHODS: In this study, 1380 medical records of patients of both genders were evaluated, ranked by applying chest pain protocol using the Manchester Triage System (MTS)...
April 2, 2018: Journal of Clinical Laboratory Analysis
Gopeekrishnan Sreenilayam, Eric J Moore, Viktoria Steck, Rudi Fasan
Engineered myoglobins have recently emerged as promising scaffolds for catalyzing carbene-mediated transformations. In this work, we investigated the effect of altering the metal center and its first-sphere coordination environment on the carbene transfer reactivity of myoglobin. To this end, we first established an efficient protocol for the recombinant expression of myoglobin variants incorporating metalloporphyrins with non-native metals, including second- and third-row transition metals (ruthenium, rhodium, iridium)...
June 19, 2017: Advanced Synthesis & Catalysis
Rahul Sakla, D Amilan Jose
In this paper, a new type of methodology to deliver carbon monoxide (CO) for biological application has been introduced. Amphiphilic manganese carbonyl complex (1.Mn) incorporated into the DSPC lipid vesicles have been reported first time for the photo induce release of CO. The liposomes (Ves-1.Mn) gradually released CO under light at 365 nm over a period of 50 min with half-time of 26.5 min. The CO-releasing ability of vesicles appended 1.Mn complexes have been confirmed by myoglobin assay and IR study. The vesicles appended with 1...
March 30, 2018: ACS Applied Materials & Interfaces
Dan Fransson, Jeppe Foged Vigh-Larsen, Ioannis G Fatouros, Peter Krustrup, Magni Mohr
We examined the degree of post-game fatigue and the recovery pattern in various leg and upper-body muscle groups after a simulated soccer game. Well-trained competitive male soccer players (n = 12) participated in the study. The players completed the Copenhagen Soccer Test, a 2 x 45 min simulated soccer protocol, following baseline measures of maximal voluntary contractions of multiple muscle groups and systemic markers of muscle damage and inflammation at 0, 24 and 48 h into recovery. All muscle groups had a strength decrement ( p ≤ 0...
March 2018: Journal of Human Kinetics
Suvi T Vaara, Rinaldo Bellomo
Continuous renal replacement therapy (CRRT) has many potential indications that extend beyond the traditional removal of uremic waste products. Many of these stem from the capacity of CRRT to remove middle-sized molecules and its suitability for haemodynamically unstable patients. Using CRRT for immunomodulation to remove circulating plasma cytokines has created great enthusiasm and, in the past, immunomodulation was a frequently reported indication for commencing CRRT. Unfortunately, recent evidence from randomized controlled trials has been discouraging, and CRRT cannot be recommended for immunomodulation...
2018: Contributions to Nephrology
Jiří Vencovský
Idiopathic inflammatory myopathies form a heterogeneous group of acquired inflammatory diseases afflicting striated muscles. The disease is frequently accompanied by systemic and organ involvement. Dermatomyositis, polymyositis, cancer associated myositis, immune mediated necrotizing myopathy, myositis in overlap syndromes, juvenile myositis and inclusion body myositis are the most frequently encountered subtypes. The basic manifestation is usually painless muscle weakness brought about by inflammation and by other immune changes at the impacted muscles...
2018: Vnitr̆ní Lékar̆ství
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