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https://www.readbyqxmd.com/read/29052941/a-method-for-extracting-carboxy-myoglobin-from-beef
#1
Masahiro Waga, Shiro Takeda, Ryoichi Sakata
The present study was conducted to devise a method for the effective extraction of carboxy-myoglobin (COMb) from beef without carbon monoxide dissociation. The ratio of COMb to myoglobin was computed at absorptions of wavelengths 541 and 551 nm, which characterize COMb and the isosbestic point between COMb and deoxy-myoglobin, respectively. The COMb extraction rate was found to vary with temperature, pH and oxygen conditions. The decrease observed in this rate was inversely proportional to the rise in extraction temperature...
October 20, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/29050512/iron-deficiency-beyond-erythropoiesis-should-we-be-concerned
#2
Khaled M Musallam, Ali T Taher
OBJECTIVE: To consider the key implications of iron deficiency for biochemical and physiological functions beyond erythropoiesis. METHODS: PubMed was searched for relevant journal articles published up to August 2017. RESULTS: Anemia is the most well-recognized consequence of persisting iron deficiency, but various other unfavorable consequences can develop either before or concurrently with anemia. Mitochondrial function can be profoundly disturbed since iron is a cofactor for heme-containing enzymes and non-heme iron-containing enzymes in the mitochondrial electron transport chain...
October 20, 2017: Current Medical Research and Opinion
https://www.readbyqxmd.com/read/29040977/gene-microarray-integrated-with-high-throughput-proteomics-for-the-discovery-of-transthyretin-in-rhabdomyolysis-induced-acute-kidney-injury
#3
Ou Li, Xiaodong Geng, Qian Ma, Weiwei Wang, Ran Liu, Zhong Yin, Siyang Wang, Guangyan Cai, Xiangmei Chen, Quan Hong
BACKGROUND/AIMS: Rhabdomyolysis, one of the leading causes of acute kidney injury (AKI), develops after trauma, drug toxicity, infections, burns, and physical exertion. The aim of this study was to investigate differences in gene and protein expression to elucidate the pathogenesis of rhabdomyolysis (RM)-induced AKI. METHODS: In this study, we used glycerol induced renal injury as a model of RM-induced AKI. Affymetrix U133 plus 2.0 microarrays were used to perform gene microarray analysis...
October 18, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29040663/heart-type-fatty-acid-binding-protein-h-fabp-and-highly-sensitive-troponin-t-hstnt-as-markers-of-myocardial-injury-and-cardiovascular-events-in-elective-percutaneous-coronary-intervention-pci
#4
M Connolly, J Shand, M Kinnin, I Menown, M J Kurth, J Lamont, D Mc Eneaney
OBJECTIVES: To assess the prognostic value of biomarkers of myocardial injury following elective percutaneous coronary intervention (PCI). BACKGROUND: Type 4a myocardial infarction (MI) occurs when myocardial injury is combined with either symptoms suggestive of myocardial ischaemia, new left bundle branch block, angiographic loss of patency of a major artery or imaging suggestive of new loss of myocardium. Myocardial injury is defined as a rise of > 5 x 99th upper reference limit (URL) of 14ng/L (i...
October 10, 2017: QJM: Monthly Journal of the Association of Physicians
https://www.readbyqxmd.com/read/29037328/xyloglucan-intake-attenuates-myocardial-injury-by-inhibiting-apoptosis-and-improving-energy-metabolism-in-a-rat-model-of-myocardial-infarction
#5
Sun Ha Lim, Jongwon Lee
The development of coronary heart disease can be divided into preocclusion and postocclusion steps. We previously showed that cell wall polysaccharides consisting of a high content of arabinose and/or xylose, such as apple pectin, protected against myocardial injury by inhibiting postocclusion steps. We hypothesized that xyloglucan, another apple cell wall polysaccharide that consists of a high content of xylose, might also show myocardial protection. To test the hypothesis, rats were supplemented with either tamarind xyloglucan (TXG) (1, 10, and 100 mg/kg per day) or cotton cellulose (CCL) (100mg/kg per day) for 3 days...
September 2017: Nutrition Research
https://www.readbyqxmd.com/read/29034393/centrifugal-micropipette-tip-with-pressure-signal-readout-for-portable-quantitative-detection-of-myoglobin
#6
Yanling Song, Yuan An, Weizhi Liu, Wanfu Hou, Xingrui Li, Bingqian Lin, Zhi Zhu, Shengxiang Ge, Huang-Hao Yang, Chaoyong Yang
This paper describes a centrifugal micropipette-tip method for ELISA sample processing combined with a pressure meter for portable quantitative detection of acute myocardial infarction (AMI) biomarker myoglobin (Myo). The method enables sensitive and reliable quantification of Myo in 35 minutes. With the advantages of simplicity, speed, user friendliness, low cost, and small sample consumption, centrifugal micropipette-tip ELISA shows great potential for quantitative POC diagnostics for AMI.
October 16, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/29032393/acute-effects-of-repeated-cycling-sprints-in-hypoxia-induced-by-voluntary-hypoventilation
#7
Xavier Woorons, Patrick Mucci, Julien Aucouturier, Agathe Anthierens, Grégoire P Millet
PURPOSE: This study aimed to investigate the acute responses to repeated-sprint exercise (RSE) in hypoxia induced by voluntary hypoventilation at low lung volume (VHL). METHODS: Nine well-trained subjects performed two sets of eight 6-s sprints on a cycle ergometer followed by 24 s of inactive recovery. RSE was randomly carried out either with normal breathing (RSN) or with VHL (RSH-VHL). Peak (PPO) and mean power output (MPO) of each sprint were measured. Arterial oxygen saturation, heart rate (HR), gas exchange and muscle concentrations of oxy-([O2Hb]) and deoxyhaemoglobin/myoglobin ([HHb]) were continuously recorded throughout exercise...
October 14, 2017: European Journal of Applied Physiology
https://www.readbyqxmd.com/read/29023404/novel-synthesis-of-core-shell-silica-nanoparticles-for-the-capture-of-low-molecular-weight-proteins-and-peptides
#8
Sergio G Hernandez-Leon, Jose Andre-I Sarabia-Sainz, Gabriela Ramos-Clamont Montfort, Ana M Guzman-Partida, Maria Del Refugio Robles-Burgueño, Luz Vazquez-Moreno
Silica nanoparticles were functionalized with immobilized molecular bait, Cibacron Blue, and a porous polymeric bis-acrylamide shell. These nanoparticles represent a new alternative to capture low molecular weight (LMW) proteins/peptides, that might be potential biomarkers. Functionalized core-shell silica nanoparticles (FCSNP) presented a size distribution of 243.9 ± 11.6 nm and an estimated surface charge of -38.1 ± 0.9 mV. The successful attachment of compounds at every stage of synthesis was evidenced by ATR-FTIR...
October 12, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29020021/physiological-morphological-and-ecological-tradeoffs-influence-vertical-habitat-use-of-deep-diving-toothed-whales-in-the-bahamas
#9
Trevor W Joyce, John W Durban, Diane E Claridge, Charlotte A Dunn, Holly Fearnbach, Kim M Parsons, Russel D Andrews, Lisa T Ballance
Dive capacity among toothed whales (suborder: Odontoceti) has been shown to generally increase with body mass in a relationship closely linked to the allometric scaling of metabolic rates. However, two odontocete species tagged in this study, the Blainville's beaked whale Mesoplodon densirostris and the Cuvier's beaked whale Ziphius cavirostris, confounded expectations of a simple allometric relationship, with exceptionally long (mean: 46.1 min & 65.4 min) and deep dives (mean: 1129 m & 1179 m), and comparatively small body masses (med...
2017: PloS One
https://www.readbyqxmd.com/read/28992849/severe-myoglobinuric-acute-kidney-injury-in%C3%A2-a%C3%A2-kidney-recipient-rapid-recovery-after-hemodialysis-with-the-super-high-flux-membrane-theralite%C3%A2-%C3%A2
#10
Ahmed Goubella, Fabrice Gankam-Kengne, Thomas Baudoux, David Fagnoul, Cécile Husson, Marie-Luce Delforge, Nilufer Broeders, Joëlle Nortier
Myoglobinuric acute kidney injury (AKI) is a severe condition requiring early therapeutic strategies. Early recognition and treatment are crucial to reduce morbidity and mortality rate. Here, we report a kidney recipient with severe rhabdomyolysis and AKI secondary to parvovirus B19 infection. Initiation of hemodialysis with the super high-flux filter Theralite® (Gambro, cut-off 45 kDa, 2.1 m2) resulted in the clearance of myoglobin from 61 to 71% after 3 hours. Elimination rates of IL-6 and β2-microglobulin were ~ 30 - 64% and 55 - 71% after 3 hours, respectively...
October 10, 2017: Clinical Nephrology
https://www.readbyqxmd.com/read/28990205/developing-a-protocol-for-normothermic-ex-situ-limb-perfusion
#11
Eliana F R Duraes, Maria Madajka, Russell Frautschi, Basem Soliman, Cagri Cakmakoglu, Addison Barnett, Kashyap Tadisina, Qiang Liu, Patrick Grady, Cristiano Quintini, Toshihiro Okamoto, Francis Papay, Antonio Rampazzo, Bahar Bassiri Gharb
BACKGROUND: Ischemia time represents a significant limitation for successful extremity transplantation because of the rapid deterioration of ischemic muscle. Normothermic ex-situ preservation is an emergent method to prolong the organ viability following procurement, by replicating the physiologic conditions. The aim of this study was to develop an ex-situ normothermic limb perfusion system to preserve the viability and function of porcine limbs for 12 hours following procurement. METHODS: A total of 18 swine limbs were perfused...
October 9, 2017: Microsurgery
https://www.readbyqxmd.com/read/28985593/effects-of-chilled-then-frozen-storage-up-to-52weeks-on-an-indicator-of-protein-oxidation-and-indices-of-protein-degradation-in-lamb-m-longissimus-lumborum
#12
Cassius E O Coombs, Benjamin W B Holman, Damian Collins, Matthew J Kerr, Michael A Friend, David L Hopkins
This study investigated the protein oxidation properties of lamb following chilled-then-frozen storage. Experimental (n=360) M. longissimus lumborum (LL) were randomly sampled from the boning room of a commercial Australian abattoir, at 24h post mortem, and assigned to five chilled storage periods (0, 2, 4, 6 and 8weeks) and six subsequent frozen storage periods (0, 4, 8, 12, 24 and 52weeks). Upon completion of each storage treatment combination, corresponding LL were sub-sectioned and analysed for carbonyl content, protein solubility, nitrate/nitrite content, particle size analysis and estimated myoglobin fractions...
September 28, 2017: Meat Science
https://www.readbyqxmd.com/read/28984134/ultrafast-co-kinetics-in-heme-proteins-adiabatic-ligand-binding-and-heavy-atom-tunneling
#13
Abdelkrim Benabbas, Yuhan Sun, Thomas L Poulos, Paul M Champion
The ultrafast kinetics of CO rebinding to carbon monoxide oxidation activator protein (ChCooA) are measured over a wide temperature range and compared with the kinetics of CO binding in other heme systems such as myoglobin (Mb) and hemoglobin (Hb). The Arrhenius prefactor for CO binding to ChCooA and protoheme (~10(11) s(-1)) is similar to what is found for spin-allowed NO binding to heme proteins and is several orders of magnitude larger than the prefactor of Mb and Hb (~10(9) s(-1)). This indicates that the CO binding reaction is adiabatic, in contrast to the commonly held view that it is non-adiabatic due to spin-forbidden (ΔS=2) selection rules...
October 6, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28982943/effects-of-exercise-training-on-calf-muscle-oxygen-extraction-and-blood-flow-in-patients-with-peripheral-artery-disease
#14
Wesley B Baker, Zhe Li, Steven S Schenkel, Malavika Chandra, David R Busch, Erin Kristine Englund, Kathryn H Schmitz, Arjun G Yodh, Thomas F Floyd, Emile R Mohler
We employed near-infrared optical techniques, diffuse correlation spectroscopy (DCS) and frequency-domain near-infrared spectroscopy (FD-NIRS), to test the hypothesis that supervised exercise training increases skeletal muscle microvascular blood flow and oxygen extraction in patients with peripheral artery disease (PAD) who experience claudication. PAD patients (n=64) were randomly assigned to exercise and control groups. Patients in the exercise group received three months of supervised exercise training...
October 5, 2017: Journal of Applied Physiology
https://www.readbyqxmd.com/read/28974810/influence-of-modified-atmosphere-and-vacuum-packaging-with-and-without-nanosilver-coated-films-on-different-quality-parameters-of-pork
#15
Isa Kernberger-Fischer, Corinna Kehrenberg, Guenter Klein, Dirk Schaudien, Carsten Krischek
Pork is often marketed in packages with high oxygen atmosphere (MAP) or vacuum to improve shelf life and appearance. As silver ions have antibacterial effects, food contact films coated with silver might improve the shelf life of meat. In the present study, pork was wrapped in commercially available films, coated with nanosilver particles, and stored in the two packaging variants MAP and vacuum for 12 days. During storage, samples were analyzed on days 1 (before packaging), 4, 8 and 12 for microbiological contamination, meat quality (e...
September 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28946221/comparative-profiling-of-sarcoplasmic-phosphoproteins-in-ovine-muscle-with-different-color-stability
#16
Meng Li, Zheng Li, Xin Li, Jianzeng Xin, Ying Wang, Guixia Li, Liguo Wu, Qingwu W Shen, Dequan Zhang
The phosphorylation of sarcoplasmic proteins in postmortem muscles was investigated in relationship to color stability in the present study. Although no difference was observed in the global phosphorylation level of sarcoplasmic proteins, difference was determined in the phosphorylation levels of individual protein bands from muscles with different color stability. Correlation analysis and liquid chromatography - tandem mass spectrometry (LC-MS/MS) identification of phosphoproteins showed that most of the color stability-related proteins were glycolytic enzymes...
February 1, 2018: Food Chemistry
https://www.readbyqxmd.com/read/28941688/high-ph-in-beef-longissimus-thoracis-reduces-muscle-fibre-transverse-shrinkage-and-light-scattering-which-contributes-to-the-dark-colour
#17
J Hughes, F Clarke, P Purslow, R Warner
Most studies have focused on myoglobin regarding meat colour development, with little focus on the contribution of muscle structure and light scattering. Our aim was to investigate the pH-dependent changes in muscle structure, on the light scattering properties of the meat. Beef longissimus thoracis muscles were segregated into light, medium or dark colour groups (n=18). 'Dark' muscles had a high ultimate pH (pHu), lower lightness (L*), redness (a*), higher myoglobin concentration and deoxymyoglobin content compared to other muscles...
November 2017: Food Research International
https://www.readbyqxmd.com/read/28939707/trends-in-use-of-biomarker-protocols-for-the-evaluation-of-possible-myocardial-infarction
#18
Brian J Hachey, Michael C Kontos, L Kristin Newby, Robert H Christenson, W Frank Peacock, Katherine C Brewer, James McCord
BACKGROUND: Various combinations of creatine kinase-MB, myoglobin, and cardiac troponin I or T (cTnI/cTnT) have been used to evaluate patients with suspected acute coronary syndromes. The current recommendation is to use the 99th percentile of cTnI/cTnT as the sole marker for diagnosis of acute myocardial infarction. METHODS AND RESULTS: We retrospectively analyzed cardiac marker protocols collected from 824 US hospitals undergoing Chest Pain Center Accreditation through the Society of Cardiovascular Patient Care from 2009 to 2014...
September 22, 2017: Journal of the American Heart Association
https://www.readbyqxmd.com/read/28939647/intra-cardiac-light-catheter-for-transmural-absorbance-spectroscopy-of-perfused-myocardium-measurement-of-myoglobin-oxygenation-and-mitochondria-redox-state
#19
Armel N Femnou, Sarah Kuzmiak-Glancy, Raul Covian, Abigail V Giles, Matthew W Kay, Robert S Balaban
Absorbance spectroscopy of intrinsic cardiac chromophores provides non-destructive assessment of cytosolic oxygenation and mitochondria redox state. Isolated perfused heart spectroscopy is usually conducted by collecting reflected light from the heart surface, which represents a combination of surface scattering events and light that traversed portions of the myocardium. Reflectance spectroscopy with complex surface scattering effects in the beating heart leads to difficulty in quantitating chromophore absorbance...
September 22, 2017: American Journal of Physiology. Heart and Circulatory Physiology
https://www.readbyqxmd.com/read/28935964/impact-of-mitochondrial-nitrite-reductase-on-hemodynamics-and-myocardial-contractility
#20
Peter Dungel, Carina Penzenstadler, Mostafa Ashmwe, Sergiu Dumitrescu, Tanja Stoegerer, Heinz Redl, Soheyl Bahrami, Andrey V Kozlov
Inorganic nitrite (NO2(-)) can be reduced back to nitric oxide (NO) by several heme proteins called nitrite reductases (NR) which affect both the vascular tonus and hemodynamics. The objective of this study was to clarify the impact of several NRs on the regulation of hemodynamics, for which hemodynamic parameters such as heart rate, blood pressure, arterial stiffness, peripheral resistance and myocardial contractility were characterized by pulse wave analysis. We have demonstrated that NO2(-) reduced to NO in RBCs predominantly influences the heart rate, while myoglobin (Mb) and mitochondria-derived NO regulates arterial stiffness, peripheral resistance and myocardial contractility...
September 21, 2017: Scientific Reports
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