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https://www.readbyqxmd.com/read/28443661/engineering-of-rumb-toward-a-green-catalyst-for-carbene-insertion-reactions
#1
Matthew W Wolf, David A Vargas, Nicolai Lehnert
The small, stable heme protein myoglobin (Mb) was modified through cofactor substitution and mutagenesis to develop a new catalyst for carbene transfer reactions. The native heme was removed from wild-type Mb and several Mb His64 mutants (H64D, H64A, H64V), and the resulting apoproteins were reconstituted with ruthenium mesoporphyrin IX (RuMpIX). The reconstituted proteins (RuMb) were characterized by UV-vis and circular dichroism spectroscopy and were used as catalysts for the N-H insertion of aniline derivatives and the cyclopropanation of styrene derivatives...
April 26, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/28439629/transitioning-to-the-direct-anterior-approach-in-total-hip-arthroplasty-is-it-a-true-muscle-sparing-approach-when-performed-by-a-low-volume-hip-replacement-surgeon
#2
Dan-Viorel Nistor, Sergiu Caterev, Sorana-Daniela Bolboacă, Dan Cosma, Dan Osvald Gheorghe Lucaciu, Adrian Todor
PURPOSE: We conducted this study to establish if the transition from a lateral approach (LA) to the direct anterior approach (DAA) for a low volume hip arthroplasty surgeon during the steep learning curve can be performed maintaining the muscle sparing approach of the DAA without increasing the complication rates. METHODS: In this controlled, prospective, randomized clinical study we investigated 70 patients (35 DAA, 35 LA) with similar demographics that underwent a total hip arthroplasty...
April 24, 2017: International Orthopaedics
https://www.readbyqxmd.com/read/28437706/three-phases-in-ph-dependent-heme-abstraction-from-myoglobin
#3
Sohini Mukherjee, Manjistha Mukherjee, Sabyasachi Bandyopadhyay, Abhishek Dey
The extent of heme extraction from myoglobin (Mb) by methylethyl ketone is found to be pH dependent and show three distinct phases. Parallel investigations of the protein using resonance Raman (rR) and circular dichroism (CD) across these pH regions indicate that these phases correspond to three different protonation steps in holoMb as the pH of the solution changed. The first transition occurs between pH5-6 and is due to the protonation of one of the heme propionate groups which disrupts its H-bonding with Arg 45 in the loop...
April 9, 2017: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/28432454/cobalt-tetradehydrocorrins-coordinated-by-imidazolate-like-histidine-in-the-heme-pocket-of-horseradish-peroxidase
#4
Koji Oohora, Ning Tang, Yoshitsugu Morita, Takashi Hayashi
Horseradish peroxidase was reconstituted with cobalt tetradehydrocorrin, rHRP(Co(TDHC)), as a structural analog of cobalamin coordinated with an imidazolate-like His residue, which is generally seen in native enzymes. In contrast to the previously reported cobalt tetradehydrocorrin-reconstituted myoglobin, rMb(Co(TDHC)), the HRP matrix was expected to provide strong axial ligation by His170 which has imidazolate character. rHRP(Co(II)(TDHC)) was characterized by EPR and its reaction with reductants indicates a negative shift of its redox potential compared to rMb(Co(TDHC))...
April 21, 2017: Journal of Biological Inorganic Chemistry: JBIC
https://www.readbyqxmd.com/read/28428250/functional-roles-of-globin-proteins-in-hypoxia-tolerant-ectothermic-vertebrates
#5
Angela Fago
Globins are heme-containing proteins ubiquitously expressed in vertebrates, where they serve a broad range of biological functions, directly or indirectly related to the tight control of oxygen levels and its toxic products in vivo. Perhaps the most investigated of all proteins, hemoglobin and myoglobin are primarily involved in oxygen transport and storage, but also in facilitating arterial vasodilation, suppressing mitochondrial respiration and preventing tissue oxidative damage via accessory redox enzymatic activities during hypoxia...
April 20, 2017: Journal of Applied Physiology
https://www.readbyqxmd.com/read/28424314/muscle-biochemistry-of-a-pelagic-delphinid-stenella-longirostris-longirostris-insight-into-fishery-induced-separation-of-mothers-and-calves
#6
Shawn R Noren, Kristi West
The length of time required for postnatal maturation of the locomotor muscle (longissimus dorsi) biochemistry [myoglobin (Mb) content and buffering capacity] in marine mammals typically varies with nursing duration, but it can be accelerated by species-specific behavioral demands, such as deep-diving and sub-ice transit. We examined how the swimming demands of a pelagic lifestyle influence postnatal maturation of Mb and buffering capacity in spinner dolphins (Stenella longirostris longirostris). Mb content of newborn (1...
April 15, 2017: Journal of Experimental Biology
https://www.readbyqxmd.com/read/28420988/heme-as-a-target-for-therapeutic-interventions
#7
REVIEW
Stephan Immenschuh, Vijith Vijayan, Sabina Janciauskiene, Faikah Gueler
Heme is a complex of iron and the tetrapyrrole protoporphyrin IX with essential functions in aerobic organisms. Heme is the prosthetic group of hemoproteins such as hemoglobin and myoglobin, which are crucial for reversible oxygen binding and transport. By contrast, high levels of free heme, which may occur in various pathophysiological conditions, are toxic via pro-oxidant, pro-inflammatory and cytotoxic effects. The toxicity of heme plays a major role for the pathogenesis of prototypical hemolytic disorders including sickle cell disease and malaria...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28411639/nanoarmoring-of-enzymes-by-polymer-functionalized-iron-oxide-nanoparticles
#8
Gayan Premaratne, Leslie Coats, Sadagopan Krishnan
Polymer-armored enzymes loaded onto magnetic nanoparticles, as efficient nanobioreactors with enhanced properties, are described in this chapter. Polymers are useful macromolecules carrying a large number of surface charges and repeating units of desired chemical functional groups for linking enzymes onto them. Magnetic micro/nanoparticles have been widely used as enzyme carriers with the incorporation of suitable polymer layers. Synthesized iron oxide magnetic nanoparticles have been used to immobilize a peroxide-catalyzing enzyme-like heme protein: myoglobin using covalent and noncovalent strategies...
2017: Methods in Enzymology
https://www.readbyqxmd.com/read/28409035/the-use-of-coupled-plasma-filtration-adsorption-in-traumatic-rhabdomyolysis
#9
Mario Pezzi, Silvia Renda, Anna Maria Giglio, Anna Maria Scozzafava, Simona Paola Tiburzi, Patrizia Casella, Fabrizio Iannelli, Mario Verre
Severe musculoskeletal injuries induce the release of sarcoplasmic elements such as muscle enzymes, potassium, and myoglobin in the systemic circulation. The circulating myoglobin damages the glomerulus and renal tubules. Conventional haemodialysis is not able to remove myoglobin, due to its high molecular weight (17,8 kilodaltons [kDa]). We treated four traumatic rhabdomyolysis patients with Coupled Plasma Filtration Adsorption (CPFA) in order to remove myoglobin followed by 14 hours of Continuous Veno-Venous Hemofiltration (CVVH)...
2017: Case Reports in Critical Care
https://www.readbyqxmd.com/read/28402676/optimizing-the-parameters-governing-the-fragmentation-of-cross-linked-peptides-in-a-tribrid-mass-spectrometer
#10
Lars Kolbowski, Marta Lavouras Mendes, Juri Rappsilber
We compared the five different ways of fragmentation available on a tribrid mass spectrometer and optimized their collision energies with regard to optimal sequence coverage of cross-linked peptides. We created a library of bis(sulfosuccinimidyl)suberate (BS3/DSS) cross-linked precursors, derived from the tryptic digests of three model proteins (Human Serum Albumin, creatine kinase and myoglobin). This enabled in-depth targeted analysis of the fragmentation behavior of 1065 cross-linked precursors using the five fragmentation techniques: collision-induced dissociation (CID), beam-type CID (HCD), electron-transfer dissociation (ETD), and the combinations ETciD and EThcD...
April 12, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28402191/sand-training-exercise-induced-muscle-damage-and-inflammatory-responses-to-matched-intensity-exercise
#11
Henry Brown, Brian Dawson, Martyn J Binnie, Hugh Pinnington, Marc Sim, Tristan D Clemons, Peter Peeling
This study compared markers of muscle damage and inflammation elevated by a matched-intensity interval running session on soft sand and grass surfaces. In a counterbalanced, repeated-measures and crossover design, 10 well-trained female athletes completed 2 interval-based running sessions 1 week apart on either a grass or a sand surface. Exercise heart rate (HR) was fixed at 83-88% of HR maximum. Venous blood samples were collected pre-, post- and 24 h post-exercise, and analysed for myoglobin (Mb) and C-reactive protein (CRP)...
April 12, 2017: European Journal of Sport Science
https://www.readbyqxmd.com/read/28392048/properties-of-hydrodynamic-j-type-countercurrent-chromatography-for-protein-separation-using-aqueous-two-phase-systems-with-special-reference-to-constructing-conical-columns
#12
Jianjian Ding, Shifei Li, Yuqing Zhao, Yue Hugh Guan, Li Deng, Qiuyun Deng
Protein separation using hydrodynamic countercurrent chromatography (CCC), where low backpressure is inherent, is more challenging, more time consuming and more costly when compared with separating small molecules. The most hopeful approach is to rationally design suitable columns for already commercialized J-type CCC machinery. By comparing 3 column geometries (3D helix, 2D spiral and 3D cone), we firstly constructed the mechanical model tailored to the conical column on J-type CCC using aqueous two-phase system (ATPS) on protein separation...
March 31, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28388333/blunted-myoglobin-and-quadriceps-soreness-after-electrical-stimulation-during-the-luteal-phase-or-oral-contraception
#13
Lindsey J Anderson, Lucinda L Baker, E Todd Schroeder
PURPOSE: Acute muscle damage after exercise triggers subsequent regeneration, leading to hypertrophy and increased strength after repeated exercise. It has been debated whether acute exercise-induced muscle damage is altered under various premenopausal estrogen conditions. Acute contraction-induced muscle damage was compared during exogenous (oral contraceptive, OC), endogenous (luteal phase, HI), or low (menses, LO) estrogen in healthy young women aged 21 to 30 years old. METHODS: Women (OC, n = 9; HI, n = 9; LO, n = 8; total N = 26) performed 1 neuromuscular electrical stimulation (NMES) bout...
April 7, 2017: Research Quarterly for Exercise and Sport
https://www.readbyqxmd.com/read/28383588/change-of-the-isoelectric-point-of-hemoglobin-at-the-air-water-interface-probed-by-the-orientational-flip-flop-of-water-molecules
#14
Stéphanie Devineau, Ken-Ichi Inoue, Ryoji Kusaka, Shu-Hei Urashima, Satoshi Nihonyanagi, Damien Baigl, Antonio Tsuneshige, Tahei Tahara
Elucidation of the molecular mechanisms of protein adsorption is of essential importance for further development of biotechnology. Here, we use interface-selective nonlinear vibrational spectroscopy to investigate protein charge at the air/water interface by probing the orientation of interfacial water molecules. We measured the Im χ((2)) spectra of hemoglobin, myoglobin, serum albumin and lysozyme at the air/water interface in the CH and OH stretching regions using heterodyne-detected vibrational sum frequency generation (HD-VSFG) spectroscopy, and we deduced the isoelectric point of the protein by monitoring the orientational flip-flop of water molecules at the interface...
April 19, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28375148/low-dose-fixed-target-serial-synchrotron-crystallography
#15
Robin L Owen, Danny Axford, Darren A Sherrell, Anling Kuo, Oliver P Ernst, Eike C Schulz, R J Dwayne Miller, Henrike M Mueller-Werkmeister
The development of serial crystallography has been driven by the sample requirements imposed by X-ray free-electron lasers. Serial techniques are now being exploited at synchrotrons. Using a fixed-target approach to high-throughput serial sampling, it is demonstrated that high-quality data can be collected from myoglobin crystals, allowing room-temperature, low-dose structure determination. The combination of fixed-target arrays and a fast, accurate translation system allows high-throughput serial data collection at high hit rates and with low sample consumption...
April 1, 2017: Acta Crystallographica. Section D, Structural Biology
https://www.readbyqxmd.com/read/28369976/quantum-chemical-calculations-of-tryptophan-%C3%A2-heme-electron-and-excitation-energy-transfer-rates-in-myoglobin
#16
Christian J Suess, Jonathan D Hirst, Nicholas A Besley
The development of optical multidimensional spectroscopic techniques has opened up new possibilities for the study of biological processes. Recently, ultrafast two-dimensional ultraviolet spectroscopy experiments have determined the rates of tryptophan → heme electron transfer and excitation energy transfer for the two tryptophan residues in myoglobin (Consani et al., Science, 2013, 339, 1586). Here, we show that accurate prediction of these rates can be achieved using Marcus theory in conjunction with time-dependent density functional theory...
April 1, 2017: Journal of Computational Chemistry
https://www.readbyqxmd.com/read/28369518/misregulation-of-calcium-handling-proteins-promotes-hyperactivation-of-calcineurin-nfat-signaling-in-skeletal-muscle-of-dm1-mice
#17
Aymeric Ravel-Chapuis, Guy Bélanger, Jocelyn Côté, Robin N Michel, Bernard J Jasmin
Myotonic Dystrophy type 1 (DM1) is caused by an expansion of CUG repeats in DMPK mRNAs. This mutation affects alternative splicing through misregulation of RNA-binding proteins. Amongst pre-mRNAs that are misspliced, several code for proteins involved in calcium homeostasis suggesting that calcium-handling and signaling are perturbed in DM1. Here, we analyzed expression of such proteins in DM1 mouse muscle. We found that the levels of several sarcoplasmic reticulum proteins (SERCA1, sarcolipin and calsequestrin) are altered, likely contributing to an imbalance in calcium homeostasis...
March 27, 2017: Human Molecular Genetics
https://www.readbyqxmd.com/read/28368957/impact-of-endurance-exercise-in-hypoxia-on-muscle-damage-inflammatory-and-performance-responses
#18
Daichi Sumi, Chihiro Kojima, Kazushige Goto
This study evaluated muscle damage and inflammatory and performance responses following high-intensity endurance exercise in moderate hypoxia among endurance athletes. Nine trained endurance athletes completed two different trials on different days: exercise under moderate hypoxia (H trial, FiO2 = 14.5%) and normoxia (N trial, FiO2 = 20.9%). They performed interval exercises (10 × 3-min running at 95% of VO2max with 60-s of active rest at 60% of VO2max) followed by 30-min of continuous running at 85% of VO2max under either hypoxic or normoxic conditions...
March 25, 2017: Journal of Strength and Conditioning Research
https://www.readbyqxmd.com/read/28366001/highly-diastereo-and-enantioselective-synthesis-of-trifluoromethyl-substituted-cyclopropanes-via-myoglobin-catalyzed-transfer-of-trifluoromethylcarbene
#19
Antonio Tinoco, Viktoria Steck, Vikas Tyagi, Rudi Fasan
We report an efficient strategy for the asymmetric synthesis of trifluoromethyl-substituted cyclopropanes by means of myoglobin-catalyzed olefin cyclopropanation reactions in the presence of 2-diazo-1,1,1-trifluoroethane (CF3CHN2) as the carbene donor. These transformations were realized using a two-compartment setup in which ex situ generated gaseous CF3CHN2 is processed by engineered myoglobin catalysts expressed in bacterial cells. This approach was successfully applied to afford a variety of trans-1-trifluoromethyl-2-arylcyclopropanes in high yields (61-99%) and excellent diastereo- and enantioselectivity (97-99...
April 10, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28363495/nitric-oxide-release-by-deoxymyoglobin-nitrite-reduction-during-cardiac-ischemia-a-mathematical-model
#20
Yien Liu, Donald G Buerk, Kenneth A Barbee, Dov Jaron
Interactions between cardiac myoglobin (Mb), nitrite, and nitric oxide (NO) are vital in regulating O2 storage, transport, and NO homeostasis. Production of NO through the reduction of endogenous myocardial nitrite by deoxygenated myoglobin has been shown to significantly reduce myocardial infarction damage and ischemic injury. We developed a mathematical model for a cardiac arteriole and surrounding myocardium to examine the hypothesis that myoglobin switches functions from being a strong NO scavenger to an NO producer via the deoxymyoglobin nitrite reductase pathway...
March 28, 2017: Microvascular Research
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