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https://www.readbyqxmd.com/read/27912143/coupling-of-helix-e-f-motion-with-the-o-nitrito-and-2-nitrovinyl-coordination-in-myoglobin
#1
Androulla Ioannou, Alexandra Lambrou, Vangelis Daskalakis, Eftychia Pinakoulaki
Myoglobin (Mb) is known to react slowly with nitirite to form the green pigment by NO2(-) cordination to the heme Fe in the O-binding nitrito (O1NO2) mode and to the heme 2-vinyl position. Nitrite is a powerful oxidizing agent and a biological reservoir for NO that has been implicated in a variety of aerobic biological systems. Accordingly, it is important to elucidate the nature and variety of NO2(-) reaction mechanisms with Mb. We have performed principal component analysis (PCA, or essential dynamics) on Molecular Dynamics trajectories of all MbNO2 coordination states to resolve the most important motions in the protein at 298K...
November 25, 2016: Biophysical Chemistry
https://www.readbyqxmd.com/read/27898916/effects-of-aging-on-the-fundamental-color-chemistry-of-dark-cutting-beef
#2
A R English, K M Wills, B N Harsh, G G Mafi, D L VanOverbeke, R Ramanathan
The objective of the current study was to evaluate the effects of aging on myoglobin chemistry of dark-cutting beef. Ten USDA Choice (mean pH = 5.6; normal pH beef) and 10 no-roll dark cutter (mean pH = 6.4) strip loins were obtained from a commercial packing plant within 3 d of harvest. Loins were cut into 4 sections, vacuum packaged, randomly assigned to 0-, 21-, 42-, and 62-d aging at 2°C in the dark. Following aging, loin sections were cut into 2.5-cm-thick steaks and were used to determine bloom development, oxygen consumption (OC), metmyoglobin reducing activity (MRA), and lipid oxidation...
September 2016: Journal of Animal Science
https://www.readbyqxmd.com/read/27898846/rapid-communication-impact-of-contemporary-light-sources-on-oxidation-of-fresh-ground-beef
#3
J V Cooper, B R Wiegand, A B Koc, L Schumacher, I Grün, C L Lorenzen
Meat color is considered one of the driving factors in consumer purchasing decisions. The objective of this study was to determine the impact of 2 different lighting sources on color and lipid oxidation of ground beef patties in a controlled environment. USDA Select top rounds ( = 20) were processed to produce ground beef at 2 different fat levels (5 and 25%) and made into patties (113.4 g). Patties were packaged with oxygen permeable polyvinyl chloride, assigned to one of three lighting treatments (low UV fluorescent [FLO], light emitting diode [LED], and no light [DRK, negative control]), and placed within deli cases at 5°C...
October 2016: Journal of Animal Science
https://www.readbyqxmd.com/read/27891064/proteomic-based-approach-for-characterizing-4-hydroxy-2-nonenal-induced-oxidation-of-buffalo-bubalus-bubalis-and-goat-capra-hircus-meat-myoglobins
#4
Naveena B Maheswarappa, K Usha Rani, Y Praveen Kumar, Vinayak V Kulkarni, Srikanth Rapole
BACKGROUND: Myoglobin (Mb) is a sarcoplasmic heme protein primarily responsible for meat color and its chemistry is species specific. 4-hydroxy-2-nonenal (HNE) is a cytotoxic lipid derived aldehyde detected in meat and was reported to covalently adduct with nucleophilic histidine residues of Mb and predispose it to greater oxidation. However, no literature is available on characterization of lipid oxidation induced oxidation of Indian water buffalo (Bubalus bubalis) and goat (Capra hircus) myoglobins...
2016: Proteome Science
https://www.readbyqxmd.com/read/27888781/stabilization-of-cytochrome-b5-by-a-conserved-tyrosine-in-the-secondary-sphere-of-heme-active-site-a-spectroscopic-and-computational-study
#5
Shan Hu, Bo He, Xiao-Juan Wang, Shu-Qin Gao, Ge-Bo Wen, Ying-Wu Lin
Heme proteins perform a large array of biological functions, with the heme group bound non-covalently or covalently. To probe the stabilization role of conserved tyrosine residue in the secondary sphere of heme site in heme proteins, we herein used cytochrome b5 (Cyt b5) as a model protein, and mutated Tyr30 to Phe or His by removal of Tyr30 associated H-bond network and hydrophobic interaction. We performed thermal-induced unfolding studies for the two mutants, Y30F Cyt b5 and Y30H Cyt b5, as monitored by both UV-Vis and CD spectroscopy, as well as heme transfer studies from these proteins to apo-myoglobin, with wild-type Cyt b5 under the same conditions for comparison...
November 20, 2016: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/27886384/valorization-of-functional-properties-of-extract-and-powder-of-olive-leaves-in-raw-and-cooked-minced-beef-meat
#6
Fathia Aouidi, Aicha Okba, Moktar Hamdi
BACKGROUND: Olive leaves (OL), resulting with a huge amount from pruning, were known to be a useful source for biologically active compounds. This paper investigates the potential application of OL as a supplement to minced beef meat, in order to develop a functional product. The effect of OL extract or powder (100, 150 µg phenols g(-1) of meat) on quality and stability of raw and cooked meat, during refrigerated storage, was examined. RESULTS: Microwave drying at 600w gave OL with the highest antioxidant quality (evaluated by TEACphenols and by DPPHphenols , compared to other methods...
November 25, 2016: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/27885768/gram-scale-synthesis-of-chiral-cyclopropane-containing-drugs-and-drug-precursors-with-engineered-myoglobin-catalysts-featuring-complementary-stereoselectivity
#7
Priyanka Bajaj, Gopeekrishnan Sreenilayam, Vikas Tyagi, Rudi Fasan
Engineered hemoproteins have recently emerged as promising systems for promoting asymmetric cyclopropanations, but variants featuring predictable, complementary stereoselectivity in these reactions have remained elusive. In this study, a rationally driven strategy was implemented and applied to engineer myoglobin variants capable of providing access to 1-carboxy-2-aryl-cyclopropanes with high trans-(1R,2R) selectivity and catalytic activity. The stereoselectivity of these cyclopropanation biocatalysts complements that of trans-(1S,2S)-selective variants developed here and previously...
November 25, 2016: Angewandte Chemie
https://www.readbyqxmd.com/read/27884955/effect-of-exercise-induced-muscle-damage-on-vascular-function-and-skeletal-muscle-microvascular-deoxygenation
#8
Jacob T Caldwell, Garrett C Wardlow, Patrece A Branch, Macarena Ramos, Christopher D Black, Carl J Ade
This paper investigated the effects of unaccustomed eccentric exercise-induced muscle damage (EIMD) on macro- and microvascular function. We tested the hypotheses that resting local and systemic endothelial-dependent flow-mediated dilation (FMD) and microvascular reactivity would decrease, V˙O2max would be altered, and that during ramp exercise, peripheral O2 extraction, evaluated via near-infrared-derived spectroscopy (NIRS) derived deoxygenated hemoglobin + myoglobin ([HHb]), would be distorted following EIMD In 13 participants, measurements were performed prior to (Pre) and 48 h after a bout of knee extensor eccentric exercise designed to elicit localized muscle damage (Post)...
November 2016: Physiological Reports
https://www.readbyqxmd.com/read/27883957/effect-of-freezing-prior-to-aging-on-myoglobin-redox-forms-and-cie-color-of-beef-from-nellore-and-aberdeen-angus-cattle
#9
Carolina Naves Aroeira, Robledo de Almeida Torres Filho, Paulo Rogério Fontes, Alcinéia de Lemos Souza Ramos, Lúcio Alberto de Miranda Gomide, Márcio Machado Ladeira, Eduardo Mendes Ramos
The objective of this study was to evaluate the effect of freezing prior to wet aging on the color of Nellore and Aberdeen Angus cattle meat. Samples of the Longissimus thoracis muscle were subjected to two treatments: conventional aging (0, 7, 14 and 21days); and freezing (-20°C for 40days) followed by thawing and aging. Freezing promoted (P<0.05) formation of metmyoglobin during aging, especially in Nellore beef. Frozen meats showed (P<0.05) lower lightness (L*) values and higher redness (a*), chroma (C*) and hue angle (h*) values at the first day of storage, deteriorating quickly with aging time...
November 16, 2016: Meat Science
https://www.readbyqxmd.com/read/27882358/monofloral-honeys-by-sicilian-black-honeybee-apis-mellifera-ssp-sicula-have-high-reducing-power-and-antioxidant-capacity
#10
Alessandro Attanzio, Luisa Tesoriere, Mario Allegra, Maria A Livrea
Thirty samples from thirteen Sicilian monofloral honeys by the local black honeybee, and two honeydew honeys, were studied to assess phenol content, reducing power and antioxidant capacity as well as correlations among these parameters. Honeys from Apiaceae showed the highest phenol amount and capacity to reduce ferric ion and stable chemical radicals, whereas honeys from Leguminosae the lowest. All honeys were active against myoglobin-derived radicals usually formed in red meat after storage and/or heating and significant correlation (p = 0...
November 2016: Heliyon
https://www.readbyqxmd.com/read/27880013/on-demand-release-of-hydrosoluble-drugs-from-a-paramagnetic-porous-collagen-based-scaffold
#11
Simona Bettini, Valentina Bonfrate, Marta Madaghiele, Luca Salvatore, Zois Syrgiannis, Gabriele Giancane, Ludovico Valli
The specific design of a collagen scaffold containing iron oxide nanostructures capped by a TiO2 (anatase) layer is reported. The TiO2 shell is proposed with a dual role: as an innovative and biocompatible cross-linker agent, providing binding sites to the protein moiety, through the well-known TiO2 chemical affinity towards carboxyl groups, and as a protective surface layer from oxidation for the paramagnetic core. Simultaneously, the presence of the nanostructures confers to the collagen gel the sensitivity to an external stimulus, i...
November 23, 2016: Chemistry: a European Journal
https://www.readbyqxmd.com/read/27879408/a-dual-label-time-resolved-fluorescence-immunoassay-for-the-simultaneous-determination-of-cardiac-troponin-t-and-myoglobin
#12
Ling Wang, Mujuan Xu, Ruolan Huang, Xiao Chang, Cuicui Chen, Laiqing Li, Zhong Zhang, Yue Han
The aim of this study was to establish a dual-label time-resolved fluorescence immunoassay (TRFIA) for the simultaneous determination of cardiac troponin T (cTnT) and myoglobin (MYO) for the early diagnosis of acute myocardial infarction. The sandwich immunoassay was used to detect the concentration of cTnT and MYO in serum. cTnT and MYO in serum were captured by anti-cTnT and anti-MYO antibodies immobilized on microtiter wells and then banded together with another anti-cTnT and anti-MYO labeled with europium(III) Sm(3+) and samarium(III) Eu(3+) chelates, followed by fluorescence measurement using time-resolved fluorometry...
November 22, 2016: Journal of Laboratory Automation
https://www.readbyqxmd.com/read/27874865/direct-observation-of-subpicosecond-vibrational-dynamics-in-photoexcited-myoglobin
#13
C Ferrante, E Pontecorvo, G Cerullo, M H Vos, T Scopigno
Determining the initial pathway for ultrafast energy redistribution within biomolecules is a challenge, and haem proteins, for which energy can be deposited locally in the haem moiety using short light pulses, are suitable model systems to address this issue. However, data acquired using existing experimental techniques that fail to combine sufficient structural sensitivity with adequate time resolution have resulted in alternative hypotheses concerning the interplay between energy flow among highly excited vibrational levels and potential concomitant electronic processes...
December 2016: Nature Chemistry
https://www.readbyqxmd.com/read/27868374/automated-coating-procedures-to-produce-poly-ethylene-glycol-brushes-in-fused-silica-capillaries
#14
Nicklas N Poulsen, Jesper Østergaard, Nickolaj J Petersen, Kim Daasbjerg, Joseph Iruthayaraj, Andra Dedinaite, Ricardas Makuska, Henrik Jensen
Many bioanalytical methods rely on electrophoretic separation of structurally labile and surface active biomolecules such as proteins and peptides. Often poor separation efficiency is due to surface adsorption processes leading to protein denaturation and surface fouling in the separation channel. Flexible and reliable approaches for preventing unwanted protein adsorption in separation science are thus in high demand. We therefore present new coating approaches based on an automated in-capillary surface initiated atom transfer radical polymerization process (covalent coating) as well as by electrostatically adsorbing a pre-synthesized polymer leading to functionalized molecular brushes...
November 21, 2016: Journal of Separation Science
https://www.readbyqxmd.com/read/27867483/brain-globins-in-physiology-and-pathology
#15
REVIEW
Luo-Kun Xie, Shao-Hua Yang
Globins are globular proteins for either transport or storage of oxygen which are critical for cellular metabolism. Four globins have been identified in rodent and human brains. Among them, neuroglobin, cytoglobin and hemoglobin chains are constitutively expressed in normal brain, while myoglobin is only expressed in some neurological disorders. Studies on the molecular structure, expression and functional features of these brain globins indicated that they may play crucial roles in maintenance of neural cell survival and activity, including neurons and astrocytes...
July 2016: Medical Gas Research
https://www.readbyqxmd.com/read/27867424/aldehyde-and-ketone-synthesis-by-p450-catalyzed-oxidative-deamination-of-alkyl-azides
#16
Simone Giovani, Hanan Alwaseem, Rudi Fasan
Heme-containing proteins have recently attracted increasing attention for their ability to promote synthetically valuable transformations not found in nature. Following the recent discovery that engineered variants of myoglobin can catalyze the direct conversion of organic azides to aldehydes, we investigated the azide oxidative deamination reactivity of a variety of hemoproteins featuring different heme coordination environments. Our studies show that although several heme-containing enzymes possess basal activity in this reaction, an engineered variant of the bacterial cytochrome P450 CYP102A1 constitutes a particularly efficient biocatalyst for promoting this transformation, exhibiting a broad substrate scope along with high catalytic activity (up to 11,300 TON), excellent chemoselectivity, and enhanced reactivity toward secondary alkyl azides to yield ketones...
August 22, 2016: ChemCatChem
https://www.readbyqxmd.com/read/27864741/an-ultrasensitive-label-free-nanobiosensor-platform-for-the-detection-of-cardiac-biomarkers
#17
Durga Prakash Matta, Suryasnata Tripathy, Siva Rama Krishna Vanjari, Chandra Shekhar Sharma, Shiv Govind Singh
We report the fabrication of a label free nano biosensor platform comprising single nanofiber that is derived out of multi-walled carbon nanotubes (MWCNTs) embedded SU-8 photoresist, for the detection of three important human cardiac biomarkers viz., myoglobin (Myo), cardiac Troponin I (cTn I) and Creatine Kinase-MB (CK-MB). These composite nanofibers were synthesized using electrospinning process. Single nanofibers were aligned between pairs of electrodes in-situ during the electrospinning process. The target proteins were detected using chemiresistive detection methodology...
December 2016: Biomedical Microdevices
https://www.readbyqxmd.com/read/27864544/investigative-nonclinical-cardiovascular-safety-and-toxicology-studies-with-bms-986094-an-ns5b-rna-dependent-rna-polymerase-inhibitor
#18
M Gill, K Horn, J Hennan, R White, D Bounous, S Clark, J R Megill, E Janovitz, M Davies, T Sanderson, M Graziano
BMS-986094, a 2'-C-methylguanosine prodrug that was in development for treatment of chronic hepatitis C infection was withdrawn from Phase 2 clinical trials because of unexpected cardiac and renal adverse events. Investigative nonclinical studies were conducted to extend the understanding of these findings using more comprehensive endpoints. BMS-986094 was given orally to female CD-1 mice (25 and 150 mg/kg/d) for 2 weeks (53/group) and to cynomolgus monkeys (15 and 30 mg/kg/d) for up to 6 weeks (2-3/sex/group for cardiovascular safety, and 5/sex/group for toxicology)...
November 17, 2016: Toxicological Sciences: An Official Journal of the Society of Toxicology
https://www.readbyqxmd.com/read/27857546/comparison-of-meat-quality-and-fatty-acid-composition-of-longissimus-muscles-from-purebred-pigs-and-three-way-crossbred-lyd-pigs
#19
Yeong-Seok Choi, Jin-Kyu Lee, Ji-Taek Jung, Young-Chul Jung, Jong-Hyun Jung, Myung-Ok Jung, Yang-Il Choi, Sang-Keun Jin, Jung-Seok Choi
This study was conducted to find pork quality to meet the needs of consumers. Thus, the meat quality and fatty acid composition of longissimus muscles from purebred pigs (Landrace, Yorkshire, and Duroc) and three-way crossbred LYD pigs were compared and evaluated. Chemical compositions of longissimus muscles were significant (p<0.05) different among pigs. Duroc contained significant (p<0.05) higher fat contents than other pigs, whereas significant (p<0.05) higher moisture contents were observed in Landrace, Yorkshire, and LYD pigs compared to those of Duroc pigs...
October 31, 2016: Korean Journal for Food Science of Animal Resources
https://www.readbyqxmd.com/read/27846686/perspective-two-dimensional-resonance-raman-spectroscopy
#20
Brian P Molesky, Zhenkun Guo, Thomas P Cheshire, Andrew M Moran
Two-dimensional resonance Raman (2DRR) spectroscopy has been developed for studies of photochemical reaction mechanisms and structural heterogeneity in complex systems. The 2DRR method can leverage electronic resonance enhancement to selectively probe chromophores embedded in complex environments (e.g., a cofactor in a protein). In addition, correlations between the two dimensions of the 2DRR spectrum reveal information that is not available in traditional Raman techniques. For example, distributions of reactant and product geometries can be correlated in systems that undergo chemical reactions on the femtosecond time scale...
November 14, 2016: Journal of Chemical Physics
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