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Shuyun Zhu, Lei He, Fang Zhang, Mengyuan Li, Shulin Jiao, Yan Li, Mengyuan Chen, Xian-En Zhao, Hua Wang
A highly sensitive fluorimetric assay has been developed for the evaluation of glutathione reductase (GR) activity and the screening of its inhibitors by using carbon quantum dots (CQDs) as the signal reporter. The detection mechanism is based on the following facts: (1) the fluorescence of CQDs can be quenched by Hg(II) through the strong CQDs-Hg(II) coordination; (2) GR can catalyze the reduction of oxidized glutathione (GSSG) into reduced glutathione (GSH), so that the fluorescence recovery of CQDs would take place resulting from the strong GSH-Hg(II) interaction; (3) GR can lose its catalytic reduction of GSSG in the presence of its inhibitors, which will inhibit the recovery of the quenched fluorescence of CQDs...
December 1, 2016: Talanta
Meng Li, Xin Li, Jianzeng Xin, Zheng Li, Guixia Li, Yan Zhang, Manting Du, Qingwu W Shen, Dequan Zhang
The influence of protein phosphorylation on meat color stability was investigated in this study. Phosphatase and protein kinase inhibitors were added to minced ovine Longissimus thoracis et lumborum (LTL) muscle to manipulate the global phosphorylation of sarcoplasmic proteins. The data obtained show that the rate and extent of pH decline, along with lactate accumulation in postmortem muscle, were related to protein phosphorylation. Analysis of meat color and the relative content of myoglobin redox forms revealed that meat color stability was inversely related to the phosphorylation of sarcoplasmic proteins...
March 15, 2017: Food Chemistry
Sachinkumar N Ambapkar, Naresh Shetty, Arpita Dwivedy
Rhabdomyolysis is syndrome characterized by muscle necrosis which causes the release of myoglobin into the bloodstream. The manifestations of this syndrome range from asymptomatic elevation of muscle enzymes to life-threatening cases associated with extremely high enzyme levels, electrolyte imbalance and acute renal failure. Symptoms of rhabdomyolysis include dark urine, muscle weakness and fatigue. Statins are the most commonly used drugs for prevention and management of dyslipidemia. We present an interesting case report on statin induced rhabdomyolysis with renal failure...
July 2016: Journal of the Association of Physicians of India
Sree Vamsee Chintapalli, Srinivas Jayanthi, Prema L Mallipeddi, Ravi Kumar Gundampati, Suresh Kumar Thallapuranam Krishnaswamy, Damian B van Rossum, Andriy Anishkin, Sean H Adams
Previous research has indicated that long-chain fatty acids can bind myoglobin (Mb) in an oxygen dependent manner. This suggests that Oxy-Mb may play an important role in fuel delivery in Mb-rich muscle fibers (e.g., type I fibers and cardiomyocytes), and raises the possibility that Mb also serves as an acylcarnitine binding protein. We report for the first time the putative interaction and affinity characteristics for different chain lengths of both fatty acids and acylcarnitines with Oxy-Mb using molecular dynamic simulations and isothermal titration calorimetry experiments...
October 7, 2016: Journal of Biological Chemistry
Marie N Hansen, Jon O Lundberg, Mariacristina Filice, Angela Fago, Nanna M G Christensen, Frank B Jensen
In mammals, treatment with low doses of nitrite have cytoprotective effects in ischemia/reperfusion events, due to nitric oxide formation and S-nitrosation of proteins. Interestingly, anoxia-tolerant lower vertebrates possess an intrinsic ability to increase intracellular [nitrite] during anoxia in tissues with high myoglobin and mitochondria contents, such as the heart. Here we test the hypothesis that red and white skeletal muscle develops different nitrite levels in crucian carp exposed to deep hypoxia and whether this correlates with myoglobin concentration...
October 14, 2016: Journal of Experimental Biology
Joseph J Knapik, Francis G O'Connor
Exertional rhabdomyolysis (ER) is a medical condition whereby damage to skeletal muscle is induced by excessive physical activity in otherwise healthy individuals. The individual performs so much activity that he/ she presumably depletes local muscle energy stores and muscle cells are unable to maintain cellular integrity, resulting in cell damage and the release of cellular contents, with resultant secondary complications. In the military services, the incidence of ER appeared to increase in the period 2004 to 2015...
2016: Journal of Special Operations Medicine: a Peer Reviewed Journal for SOF Medical Professionals
David C Nieman, Kevin A Zwetsloot, Dominic D Lomiwes, Mary P Meaney, Roger D Hurst
The cytokine response to heavy exertion varies widely for unknown reasons, and this study evaluated the relative importance of glycogen depletion, muscle damage, and stress hormone changes on blood and muscle cytokine measures. Cyclists (N = 20) participated in a 75-km cycling time trial (168 ± 26.0 min), with blood and vastus lateralis muscle samples collected before and after. Muscle glycogen decreased 77.2 ± 17.4%, muscle IL-6, IL-8, and MCP-1 mRNA increased 18.5 ± 2.8-, 45.3 ± 7.8-, and 8.25 ± 1.75-fold, and muscle IL-6, IL-8, and MCP-1 protein increased 70...
2016: Frontiers in Physiology
Sonia B Jiménez-Pulido, Nuria A Illán-Cabeza, Francisco Hueso-Ureña, Carmen R Maldonado, Purificación Sánchez-Sánchez, M Paz Fernández-Liencres, Manuel Fernández-Gómez, Miguel N Moreno-Carretero
The synthesis, structure and CO-releasing properties of a number of new tricarbonyl rhenium(i) complexes with 5-substituted-6-amino-1,3-dimethyluracils are reported and their structural features discussed on the basis of both spectral and X-ray crystallographic analyses. The 5-substituent library includes -N[double bond, length as m-dash]CH-2py (DAAUPic) and -CH[double bond, length as m-dash]N-N[double bond, length as m-dash]CH-2py (FDUHzPic) as additional metal binding components and chloride, acetonitrile or pyridine acting as ancillary ligands...
September 27, 2016: Dalton Transactions: An International Journal of Inorganic Chemistry
Mireille Khalil, Zahia Boubegtiten-Fezoua, Nadja Hellmann, Petra Hellwig
Hemocyanins are large oligomeric respiratory proteins found in many arthropods and molluscs. Here we give infrared spectroscopic evidence of a high stability towards exposure to sub-zero temperatures for hemocyanins from the arthropods Limulus polyphemus and Eurypelma californicum at different pH values. Small but distinct temperature induced changes of the secondary structure were observed, but a stable core of at least 40% α-helical structure is preserved as identified in the infrared spectra obtained between 294 and 20 K...
October 7, 2016: Physical Chemistry Chemical Physics: PCCP
Chung-Lieh Hung, Ding-Kuo Chien, Shou-Chuan Shih, Wen-Han Chang
BACKGROUND: Accurate diagnosis of acute coronary syndrome (ACS) in a timely fashion is challenging in the elderly population, especially elderly women, who usually exhibit atypical clinical symptoms. A multiple cardiac biomarker (MCB) based approach has been shown to improve diagnostic efficacy of ACS. However, data in various age groups and sex differences remain largely unexplored. METHODS: Point-of-care testing (POCT) was performed on 290 patients (aged ≥18 years) who were admitted to the emergency department (ED) with symptoms of acute chest pain under suspicion of acute coronary syndrome (ACS)...
October 7, 2016: BMC Cardiovascular Disorders
Sara Del Galdo, Andrea Amadei
In this paper we apply the computational analysis recently proposed by our group to characterize the solvation properties of a native protein in aqueous solution, and to four model aqueous solutions of globular proteins in their unfolded states thus characterizing the protein unfolded state hydration shell and quantitatively evaluating the protein unfolded state partial molar volumes. Moreover, by using both the native and unfolded protein partial molar volumes, we obtain the corresponding variations (unfolding partial molar volumes) to be compared with the available experimental estimates...
October 12, 2016: Physical Chemistry Chemical Physics: PCCP
Biljana Kuzmanovska, Emilija Cvetkovska, Igor Kuzmanovski, Nikola Jankulovski, Mirjana Shosholcheva, Andrijan Kartalov, Tatjana Spirovska
INTRODUCTION: Rhabdomyolysis is a syndrome of injury of skeletal muscles associated with myoglobinuria, muscle weakness, electrolyte imbalance and often, acute kidney injury as severe complication. THE AIM: of this study is to detect the incidence of rhabdomyolysis in critically ill patients in the surgical intensive care unit (ICU), and to raise awareness of this medical condition and its treatment among the clinicians. MATERIAL AND METHODS: A retrospective review of all surgical and trauma patients admitted to surgical ICU of the University Surgical Clinic "Mother Teresa" in Skopje, Macedonia, from January 1(st) till December 31(st) 2015 was performed...
July 27, 2016: Medical Archives
Yuqing Yin, Chen Ling Gao, Qi Xiao, Guo Lin, Zian Lin, Zongwei Cai, Huang-Hao Yang
Artificial enzyme mimetics have received considerable attention because natural enzymes have some significant drawbacks, including enzyme autolysis, low catalytic activity, poor recovery and low stability to environmental changes. Herein, we demonstrated a facile approach for one-pot synthesis of hemeprotein-metal organic framework hybrid composites (H-MOFs) by using bovine hemoglobin (BHb) and zeolitic imidazolate framework-8 (ZIF-8) as a model reaction system. Surprisingly, the new hybrid composites exhibits 423% increase in peroxidase-like catalytic activity compared to free BHb...
October 4, 2016: ACS Applied Materials & Interfaces
Thomas R Ward
Ugrading myoglobin with iridium: A metal-substitution strategy has been used to afford a repurposed myoglobin for challenging cyclopropanation and intramolecular C-H activation reactions. The performance of the iridium-loaded myoglobin (orange sphere) was improved through directed evolution of eight active-site residues (yellow surface).
October 4, 2016: Angewandte Chemie
Xiao Xia Han, Junbo Li, Ibrahim Halil Öner, Bing Zhao, Silke Leimkühler, Peter Hildebrandt, Inez M Weidinger
Practical use of many bioelectronic and bioanalytical devices is limited by the need of expensive materials and time consuming fabrication. Here we demonstrate the use of nickel electrodes as a simple and cheap solid support material for bioelectronic applications. The naturally nanostructured electrodes showed a surprisingly high electromagnetic surface enhancement upon light illumination such that immobilization and electron transfer reactions of the model redox proteins cytochrome b5 (Cyt b5) and cytochrome c (Cyt c) could be followed via surface enhanced resonance Raman spectroscopy...
October 19, 2016: Analytica Chimica Acta
Bing Wang, Leonard M Thomas, George B Richter-Addo
Bioorganometallic Fe-C bonds are biologically relevant species that may result from the metabolism of natural or synthetic hydrazines. The molecular structures of four new sperm whale mutant myoglobin derivatives with Fe-aryl moieties, namely H64A-tolyl-m, H64A-chlorophenyl-p, H64Q-tolyl-m, and H64Q-chlorophenyl-p, have been determined at 1.7-1.9Å resolution. The structures reveal conformational preferences for the substituted aryls resulting from attachment of the aryl ligands to Fe at the site of net -NHNH2 release from the precursor hydrazines, and show distal pocket changes that readily accommodate these bulky ligands...
June 24, 2016: Journal of Inorganic Biochemistry
Yvonne Gunning, Andrew D Watson, Neil M Rigby, Mark Philo, Joshua K Peazer, E Kate Kemsley
We describe a simple protocol for identifying and quantifying the two components in binary mixtures of species possessing one or more similar proteins. Central to the method is the identification of 'corresponding proteins' in the species of interest, in other words proteins that are nominally the same but possess species-specific sequence differences. When subject to proteolysis, corresponding proteins will give rise to some peptides which are likewise similar but with species-specific variants. These are 'corresponding peptides'...
2016: Journal of Visualized Experiments: JoVE
Thomas Morville, Mads Rosenkilde, Thor Munch-Andersen, Peter Riis Andersen, Katja Kjær, Signe Helbo, Marianne Kristensen, Andreas Vigelsø, Nick Mattsson, Hanne Kruuse Rasmusen, Amelia Guadalupe-Grau, Angela Fago, Christina Neigaard Hansen, Brigitte Twelkmeyer, Jesper Løvind Andersen, Flemming Dela, Jørn Wulff Helge
INTRODUCTION/PURPOSE: Fat metabolism and muscle adaptation was investigated in 6 older trained men (age: 61 ± 4 years; VO2max: 48 ± 2 mL kg min) following repeated prolonged exercise). METHODS: 2706 km (1,681 miles) cycling was performed over 14 days and a blood sample and a muscle biopsy were obtained at rest after an overnight fast before and 30 hours after the completion of the cycling. VO2-max and maximal fat oxidation were measured using incremental exercise tests...
September 28, 2016: Medicine and Science in Sports and Exercise
A Orkusz, G Haraf, A Okruszek, M Wereńska-Sudnik
The objective of the work was to investigate the color and lipid oxidation changes of goose breast meat packaged in vacuum and modified atmosphere (MA) conditions consisting of 80% O2, 20% CO2, and stored in refrigerated conditions at 4°C. Color stability was monitored by determining total heme pigments concentration; relative concentration of myoglobin, oxymyoglobin, and metmyoglobin; parameters of color L*, a*, b*, and sensory evaluation of the surface color. Lipid stability was measured by determining thiobarbituric acid reactive substances (TBARS)...
September 24, 2016: Poultry Science
Navjot Singh Athwal, Jagannathan Alagurajan, Amy H Andreotti, Mark S Hargrove
Reduction of hydroxylamine to ammonium by phytoglobin, a plant hexacoordinate hemoglobin, is much faster than that of other hexacoordinate hemoglobins or pentacoordinate hemoglobins such as myoglobin, leghemoglobin, and red blood cell hemoglobin. The reason for differences in reactivity is not known but could be intermolecular electron transfer between protein molecules in support of the required two-electron reduction, hydroxylamine binding, or active site architecture favoring the reaction. Experiments were conducted with phytoglobins from rice, tomato, and soybean along with human neuroglobin and soybean leghemoglobin that reveal hydroxylamine binding as the rate-limiting step...
October 5, 2016: Biochemistry
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