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https://www.readbyqxmd.com/read/28338844/re-invited-commentary-beware-the-test-negative-design
#1
Jill M Ferdinands, Ivo M Foppa, Alicia M Fry, Brendan L Flannery, Edward A Belongia, Michael L Jackson
No abstract text is available yet for this article.
March 1, 2017: American Journal of Epidemiology
https://www.readbyqxmd.com/read/28335440/profile-of-polyphenol-compounds-of-five-muscadine-grapes-cultivated-in-the-united-states-and-in-newly-adapted-locations-in-china
#2
Zheng Wei, Jianming Luo, Yu Huang, Wenfeng Guo, Yali Zhang, Huan Guan, Changmou Xu, Jiang Lu
Polyphenol compositions and concentrations in skins and seeds of five muscadine grapes (cv. "Noble", "Alachua", "Carlos", "Fry", and "Granny Val") cultivated in the United States (Tallahassee-Florida, TA-FL) and South China (Nanning-Guangxi, NN-GX and Pu'er-Yunnan, PE-YN) were investigated, using ultra performance liquid chromatography tandem triple quadrupole time-of-flight mass spectrometry (UPLC Triple TOF MS/MS). Fourteen ellagitannins were newly identified in these muscadine grapes. The grapes grown in NN-GX accumulated higher levels of ellagic acid, methyl brevifolin carboxylate, and ellagic acid glucoside in skins, and penta-O-galloyl-glucose in seeds...
March 14, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28335411/how-the-cobra-got-its-flesh-eating-venom-cytotoxicity-as-a-defensive-innovation-and-its-co-evolution-with-hooding-aposematic-marking-and-spitting
#3
Nadya Panagides, Timothy N W Jackson, Maria P Ikonomopoulou, Kevin Arbuckle, Rudolf Pretzler, Daryl C Yang, Syed A Ali, Ivan Koludarov, James Dobson, Brittany Sanker, Angelique Asselin, Renan C Santana, Iwan Hendrikx, Harold van der Ploeg, Jeremie Tai-A-Pin, Romilly van den Bergh, Harald M I Kerkkamp, Freek J Vonk, Arno Naude, Morné A Strydom, Louis Jacobsz, Nathan Dunstan, Marc Jaeger, Wayne C Hodgson, John Miles, Bryan G Fry
The cytotoxicity of the venom of 25 species of Old World elapid snake was tested and compared with the morphological and behavioural adaptations of hooding and spitting. We determined that, contrary to previous assumptions, the venoms of spitting species are not consistently more cytotoxic than those of closely related non-spitting species. While this correlation between spitting and non-spitting was found among African cobras, it was not present among Asian cobras. On the other hand, a consistent positive correlation was observed between cytotoxicity and utilisation of the defensive hooding display that cobras are famous for...
March 13, 2017: Toxins
https://www.readbyqxmd.com/read/28332413/interlaboratory-validation-of-an-lc-ms-ms-method-for-the-determination-of-melamine-and-cyanuric-acid-in-animal-feed
#4
Hildburg Fry, Kerstin Mietle, Eileen Mähnert, Sebastian Zinke, Mandy Schwieters, Emanuele Pydde, Angelika Preiß-Weigert
Melamine and cyanuric acid have been mixed illegally into food and feed to increase the nitrogen content which results in deceptively high protein contents. As a consequence a maximum level for melamine of 2.5 mg kg(-1) feed was established by the European Union under directive 2002/32/EC. A method is required to quantify melamine and cyanuric acid for control of the maximum level. Therefore Technical Committee (TC) 327 of the European Committee for Standardisation (CEN) commissioned the standardisation of a method for the analysis of melamine and cyanuric acid in animal feed...
March 23, 2017: Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
https://www.readbyqxmd.com/read/28329432/user-compliance-with-documenting-on-a-track-and-trigger-based-observation-and-response-chart-a-two-phase-multi-site-audit-study
#5
Doug Elliott, Emily Allen, Sharon McKinley, Lin Perry, Christine Duffield, Margaret Fry, Robyn Gallagher, Rick Iedema, Michael Roche
AIMS: To examine user compliance and completeness of documentation with a newly designed Observation and Response Chart and whether a rapid response system call was triggered when clinically indicated. BACKGROUND: Timely recognition and responses to patient deterioration in hospital general wards remains a challenge for health care systems globally. Evaluating practice initiatives to improve recognition and response are required. DESIGN: Two-phase audit...
March 22, 2017: Journal of Advanced Nursing
https://www.readbyqxmd.com/read/28329311/comparative-effectiveness-of-high-dose-versus-standard-dose-influenza-vaccines-among-us-medicare-beneficiaries-in-preventing-postinfluenza-deaths-during-2012-2013-and-2013-2014
#6
David K Shay, Yoganand Chillarige, Jeffrey Kelman, Richard A Forshee, Ivo M Foppa, Michael Wernecke, Yun Lu, Jill M Ferdinands, Arjun Iyengar, Alicia M Fry, Chris Worrall, Hector S Izurieta
Background.: Recipients of high-dose vs standard-dose influenza vaccines have fewer influenza illnesses. We evaluated the comparative effectiveness of high-dose vaccine in preventing postinfluenza deaths during 2012-2013 and 2013-2014, when influenza viruses and vaccines were similar. Methods.: We identified Medicare beneficiaries aged ≥65 years who received high-dose or standard-dose vaccines in community-located pharmacies offering both vaccines. The primary outcome was death in the 30 days following an inpatient or emergency department encounter listing an influenza International of Classification of Diseases, Ninth Revision, Clinical Modification code...
March 2, 2017: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28322549/vitamin-c-modulates-the-immunotoxic-effect-of-17-alpha-methyltestosterone-in-nile-tilapia
#7
Haitham G Abo-Al-Ela, Abeer F El-Nahas, Shawky Mahmoud, Essam M Ibrahim
The synthetic androgen, 17 alpha-methyltestosterone (MT), is profusely used and practically needed in the production of all-male Nile tilapia fry; however, such androgenic hormones badly disrupt the immune system. This study aimed to alleviate or counteract the immunotoxic effect of MT using vitamin C (ascorbic acid, vit C). Our results show that the highest phagocytic activity (PA), phagocytic index (PI) and lysozyme activity were detected in the vit C group and the MT plus vit C group. Furthermore, PA and PI were significantly suppressed, but lysozyme activity was stronger in the MT group than the control...
March 21, 2017: Biochemistry
https://www.readbyqxmd.com/read/28320945/three-dimensional-culture-system-identifies-a-new-mode-of-cetuximab-resistance-and-disease-relevant-genes-in-colorectal-cancer
#8
Cunxi Li, Bhuminder Singh, Ramona Graves-Deal, Haiting Ma, Alina Starchenko, William H Fry, Yuanyuan Lu, Yang Wang, Galina Bogatcheva, Mohseen P Khan, Ginger L Milne, Shilin Zhao, Gregory Daniel Ayers, Nenggan Li, Huaying Hu, Mary Kay Washington, Timothy J Yeatman, Oliver G McDonald, Qi Liu, Robert J Coffey
We previously reported that single cells from a human colorectal cancer (CRC) cell line (HCA-7) formed either hollow single-layered polarized cysts or solid spiky masses when plated in 3D in type-I collagen. To begin in-depth analyses into whether clonal cysts and spiky masses possessed divergent properties, individual colonies of each morphology were isolated and expanded. The lines thus derived faithfully retained their parental cystic and spiky morphologies and were termed CC (cystic) and SC (spiky), respectively...
March 20, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28315301/metabolites-of-2-3-diketogulonate-delay-peroxidase-action-and-induce-non-enzymic-h2o2-generation-potential-roles-in-the-plant-cell-wall
#9
Anna Kärkönen, Rebecca A Dewhirst, C Logan Mackay, Stephen C Fry
A proportion of the plant's l-ascorbate (vitamin C) occurs in the apoplast, where it and its metabolites may act as pro-oxidants and anti-oxidants. One ascorbate metabolite is 2,3-diketogulonate (DKG), preparations of which can non-enzymically generate H2O2 and delay peroxidase action on aromatic substrates. As DKG itself generates several by-products, we characterised these and their ability to generate H2O2 and delay peroxidase action. DKG preparations rapidly produced a by-product, compound (1), with λmax 271 and 251 nm at neutral and acidic pH respectively...
March 14, 2017: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28298597/sat2-foot-and-mouth-disease-virus-fmdv-structurally-modified-for-increased-thermostability
#10
Katherine A Scott, Abhay Kotecha, Julian Seago, Jingshan Ren, Elizabeth E Fry, David I Stuart, Bryan Charleston, Francois F Maree
Foot-and-mouth disease virus (FMDV) is notoriously unstable, particularly the O and SAT serotypes. Consequently, vaccines derived from heat-labile SAT viruses have been linked to the induction of poor duration immunity and hence require more frequent vaccinations to ensure protection. In-silico calculations predicted residue substitutions that would increase interactions at the inter-pentameric interface supporting increased stability. We assessed the stability of the 18 recombinant mutant viruses for their growth kinetics; antigenicity; plaque morphology; genetic stability; temperature, ionic and pH stability using the thermofluor and inactivation assays, in order to evaluate potential SAT2 vaccine candidates with improved stability...
March 15, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28298232/elutriated-lymphocytes-for-manufacturing-chimeric-antigen-receptor-t-cells
#11
David F Stroncek, Daniel W Lee, Jiaqiang Ren, Marianna Sabatino, Steven Highfill, Hanh Khuu, Nirali N Shah, Rosandra N Kaplan, Terry J Fry, Crystal L Mackall
BACKGROUND: Clinical trials of Chimeric Antigen Receptor (CAR) T cells manufactured from autologous peripheral blood mononuclear cell (PBMC) concentrates for the treatment of hematologic malignancies have been promising, but CAR T cell yields have been variable. This variability is due in part to the contamination of the PBMC concentrates with monocytes and granulocytes. METHODS: Counter-flow elutriation allows for the closed system separation of lymphocytes from monocytes and granulocytes...
March 16, 2017: Journal of Translational Medicine
https://www.readbyqxmd.com/read/28289902/discovery-of-synthesis-and-secretion-of-polyol-esters-of-fatty-acids-by-four-basidiomycetous-yeast-species-in-the-order-sporidiobolales
#12
Luis A Garay, Irnayuli R Sitepu, Tomas Cajka, Oliver Fiehn, Erin Cathcart, Russell W Fry, Atit Kanti, Agustinus Joko Nugroho, Sarah Asih Faulina, Sira Stephanandra, J Bruce German, Kyria L Boundy-Mills
Polyol esters of fatty acids (PEFA) are amphiphilic glycolipids produced by yeast that could play a role as natural, environmentally friendly biosurfactants. We recently reported discovery of a new PEFA-secreting yeast species, Rhodotorula babjevae, a basidiomycetous yeast to display this behavior, in addition to a few other Rhodotorula yeasts reported on the 1960s. Additional yeast species within the taxonomic order Sporidiobolales were screened for secreted glycolipid production. PEFA production equal or above 1 g L(-1) were detected in 19 out of 65 strains of yeast screened, belonging to 6 out of 30 yeast species tested...
March 13, 2017: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28280974/post-absorptive-muscle-protein-turnover-affects-resistance-training-hypertrophy
#13
Paul T Reidy, Michael S Borack, Melissa M Markofski, Jared M Dickinson, Christopher S Fry, Rachel R Deer, Elena Volpi, Blake B Rasmussen
PURPOSE: Acute bouts of resistance exercise and subsequent training alters protein turnover in skeletal muscle. The mechanisms responsible for the changes in basal post-absorptive protein turnover and its impact on muscle hypertrophy following resistance exercise training are unknown. Our goal was to determine whether post-absorptive muscle protein turnover following 12 weeks of resistance exercise training (RET) plays a role in muscle hypertrophy. In addition, we were interested in determining potential molecular mechanisms responsible for altering post-training muscle protein turnover...
March 9, 2017: European Journal of Applied Physiology
https://www.readbyqxmd.com/read/28278147/increase-in-human-infections-with-avian-influenza-a-h7n9-virus-during-the-fifth-epidemic-china-october-2016-february-2017
#14
A Danielle Iuliano, Yunho Jang, Joyce Jones, C Todd Davis, David E Wentworth, Timothy M Uyeki, Katherine Roguski, Mark G Thompson, Larisa Gubareva, Alicia M Fry, Erin Burns, Susan Trock, Suizan Zhou, Jacqueline M Katz, Daniel B Jernigan
During March 2013-February 24, 2017, annual epidemics of avian influenza A(H7N9) in China resulted in 1,258 avian influenza A(H7N9) virus infections in humans being reported to the World Health Organization (WHO) by the National Health and Family Planning Commission of China and other regional sources (1). During the first four epidemics, 88% of patients developed pneumonia, 68% were admitted to an intensive care unit, and 41% died (2). Candidate vaccine viruses (CVVs) were developed, and vaccine was manufactured based on representative viruses detected after the emergence of A(H7N9) virus in humans in 2013...
March 10, 2017: MMWR. Morbidity and Mortality Weekly Report
https://www.readbyqxmd.com/read/28259520/derivation-and-external-validation-of-the-plasmic-score-for-rapid-assessment-of-adults-with-thrombotic-microangiopathies-a-cohort-study
#15
Pavan K Bendapudi, Shelley Hurwitz, Ashley Fry, Marisa B Marques, Stephen W Waldo, Ang Li, Lova Sun, Vivek Upadhyay, Ayad Hamdan, Andrew M Brunner, John M Gansner, Srinivas Viswanathan, Richard M Kaufman, Lynne Uhl, Christopher P Stowell, Walter H Dzik, Robert S Makar
BACKGROUND: Among the syndromes characterised by thrombotic microangiopathy, thrombotic thrombocytopenic purpura is distinguished by a severe deficiency in the ADAMTS13 enzyme. Patients with this disorder need urgent treatment with plasma exchange. Because ADAMTS13 activity testing typically requires prolonged turnaround times and might be unavailable in resource-poor settings, a method to rapidly assess the likelihood of severe ADAMTS13 deficiency is needed. METHODS: All consecutive adult patients presenting to three large academic medical centres in Boston, MA, USA, with thrombotic microangiopathy and a possible diagnosis of thrombotic thrombocytopenic purpura between Jan 8, 2004, and Dec 6, 2015, were included in an ongoing multi-institutional registry (the Harvard TMA Research Collaborative)...
March 1, 2017: Lancet Haematology
https://www.readbyqxmd.com/read/28251890/twenty-years-and-counting-epidemiology-of-an-outbreak-of-isoniazid-resistant-tuberculosis-in-england-and-wales-1995-to-2014
#16
Catherine M Smith, Suzan C M Trienekens, Charlotte Anderson, Maeve K Lalor, Tim Brown, Alistair Story, Hannah Fry, Andrew C Hayward, Helen Maguire
An outbreak of isoniazid-resistant tuberculosis first identified in London has now been ongoing for 20 years, making it the largest drug-resistant outbreak of tuberculosis documented to date worldwide. We identified culture-confirmed cases with indistinguishable molecular strain types and extracted demographic, clinical, microbiological and social risk factor data from surveillance systems. We summarised changes over time and used kernel-density estimation and k-function analysis to assess geographic clustering...
February 23, 2017: Euro Surveillance: Bulletin Européen sur les Maladies Transmissibles, European Communicable Disease Bulletin
https://www.readbyqxmd.com/read/28251652/sirt1-may-play-a-crucial-role-in-overload-induced-hypertrophy-of-skeletal-muscle
#17
Erika Koltai, Zoltán Bori, Clovis Chabert, Hervé Dubouchaud, Hisashi Naito, Shuichi Machida, Kelvin Ja Davies, Zsolt Murlasits, Andrew C Fry, Istvan Boldogh, Zsolt Radak
Significant skeletal muscle mass guarantees functional wellbeing and is important for high level performance in many sports. Although the molecular mechanism for skeletal muscle hypertrophy has been well-studied, it still is not completely understood. In the present study, we used a functional overload model to induce plantaris muscle hypertrophy by surgically removing the soleus, and gastrocnemius muscles in rats. Two weeks of muscle ablation resulted in a 40% increase in muscle mass, which was associated with a significant increase in SIRT1 content and activity (P < 0...
March 2, 2017: Journal of Physiology
https://www.readbyqxmd.com/read/28242950/effect-of-low-temperature-long-time-and-high-temperature-short-time-blanching-and-frying-treatments-on-the-french-fry-quality-of-six-irish-potato-cultivars
#18
Nomali Ziphorah Ngobese, Tilahun Seyoum Workneh, Muthulisi Siwela
Processing conditions are an important determinant of French fry quality. However, the effect of low-temperature long-time (LTLT) and high-temperature short-time (HTST) blanching and frying treatments has not been investigated in many cultivars. The current study investigates the effect of the sequential application of these treatments on French fries processed from six Irish potato cultivars (Fianna, Innovator, Mondial, Navigator, Panamera and Savanna). Blanching was effected at 75 °C for 10 min or 85 °C for 5 min and frying was effected at 160 °C for 2 min or 180 °C for 1 min...
February 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28238454/moving-toward-a-shared-process-the-impact-of-parent-experiences-on-perinatal-palliative-care
#19
Susan L Hasegawa, Jessica T Fry
Perinatal palliative care programs seek to support parents expecting a baby diagnosed with a serious medical condition. Clinicians have increasingly recognized the importance of parental perspectives on the medical care mothers and their fetuses and live-born children receive, especially regarding factors influencing individual choices and knowledge of the medical community. We describe, using literature on trisomy 13 and trisomy 18, how information shared between parents and providers can improve perinatal counseling and family support...
February 23, 2017: Seminars in Perinatology
https://www.readbyqxmd.com/read/28237051/culturally-sensitive-substance-use-treatment-for-racial-ethnic-minority-youth-a-meta-analytic-review
#20
Katarzyna T Steinka-Fry, Emily E Tanner-Smith, Gayle A Dakof, Craig Henderson
This systematic review and meta-analysis synthesized findings from studies examining culturally sensitive substance use treatment for racial/ethnic minority youth. An extensive literature search located eight eligible studies using experimental or quasi-experimental designs. The meta-analysis quantitatively synthesized findings comparing seven culturally sensitive treatment conditions to seven alternative conditions on samples composed of at least 90% racial/ethnic minority youth. The results from the meta-analysis indicated that culturally sensitive treatments were associated with significantly larger reductions in post-treatment substance use levels relative to their comparison conditions (g=0...
April 2017: Journal of Substance Abuse Treatment
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