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https://www.readbyqxmd.com/read/28944876/nutrient-deprivation-induces-apoptosis-of-nucleus-pulposus-cells-via-activation-of-the-bnip3-aif-signalling-pathway
#1
Jie Liu, Chao Yuan, Luqiao Pu, Jian Wang
Nutrient deprivation (ND)‑induced nucleus pulposus (NP) cell death serves an important role in intervertebral disc degeneration disease. However, the underlying mechanisms have yet to be thoroughly elucidated. The present study created a cell culture model under ND conditions to investigate the roles of the nutrient‑sensitive protein B‑cell lymphoma 2/adenovirus E1B 19 kDa‑interacting protein (BNIP3) and the mitochondrial pro‑death protein apoptosis‑inducing factor (AIF) in the death pathway of NP cells...
September 20, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944851/microrna%C3%A2-96-regulates-the-proliferation-of-nucleus-pulposus-cells-by-targeting-arid2-akt-signaling
#2
Bo Tao, Jiaoyu Yi, Changzhao Huang, Wang Xu, Chao Qin, Lei Chen, Jincai Chen, Yan Gao, Ruiying Wang
The aberrant proliferation of nucleus pulposus (NP) cells has been reported to be implicated in the pathogenesis of intervertebral disc degeneration (IDD). Previous studies have demonstrated that microRNAs (miRNAs), which are a group of small noncoding RNAs, are critical regulators of cell proliferation in various pathologies. However, the role of miRNA‑96 (miR‑96) in the proliferation of NP cells remains to be determined. In the present study, reverse transcription‑quantitative polymerase chain reaction was used to investigate the expression of miR‑96 in NP tissues from patients with IDD and healthy tissues from patients with traumatic lumbar fracture as the control...
September 20, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944766/magnesium-oxide-nanoparticle-loaded-polycaprolactone-composite-electrospun-fiber-scaffolds-for-bone-soft-tissue-engineering-applications-in-vitro-and-in-vivo-evaluation
#3
Ajay Suryavanshi, Kunal Khanna, K R Sindhu, Jayesh Bellare, Rohit Srivastava
The objective of the present investigation was to assess the potential of magnesium oxide nanoparticle (MgO NP)-loaded electrospun polycaprolactone (PCL) polymer composites as a bone-soft tissue engineering scaffold. MgO NPs were synthesized using a hydroxide precipitation sol-gel method and characterized using field emission gun-scanning electron microscopy/energy-dispersive x-ray spectroscopy (FEG-SEM/EDS), field emission gun-transmission electron microscopy (FEG-TEM), and x-ray diffraction (XRD) analysis...
September 25, 2017: Biomedical Materials
https://www.readbyqxmd.com/read/28944656/pt-decorated-magnetic-nanozymes-for-facile-and-sensitive-point-of-care-bioassay
#4
Min Su Kim, Soon Ho Kweon, Seongyeon Cho, Seong Soo A An, Moon Il Kim, Junsang Doh, Jinwoo Lee
Development of facile and sensitive bioassay is important for many point-of-care applications. In this study, we fulfilled such demand by synthesizing Pt-decorated magnetic nanozymes and developing a bioassay based on unique properties of the newly synthesized nanozymes. Fe3O4-Pt/Core-Shell nanoparticles (MPt/CS NPs) with various compositions were synthesized and characterized. Fe3O4 NP itself is a good nanozyme with catalytic activity superior to natural enzymes, but catalytic activity can be further improved by incorporating Pt to the outer layers of the Fe3O4 NPs and building heterogeneous nanostructures...
September 25, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28944286/tannic-acid-mediated-surface-functionalization-of-polymeric-nanoparticles
#5
Sara A Abouelmagd, Fanfei Meng, Bieong-Kil Kim, Hyesun Hyun, Yoon Yeo
Polymeric nanoparticles (NPs) are decorated with various types of molecules to control their functions and interactions with specific cells. We previously used polydopamine (pD) to prime-coat poly(lactic-co-glycolic acid) (PLGA) NPs and conjugated functional ligands onto the NPs via the pD coating. In this study, we report tannic acid (TA) as an alternative prime coating that is functionally comparable to pD but does not have drawbacks of pD such as optical properties and interference of ligand characterization...
December 12, 2016: ACS Biomaterials Science & Engineering
https://www.readbyqxmd.com/read/28943482/atrial-fibrillation-and-cognitive-decline-in-the-framingham-heart-study
#6
Arvind Nishtala, Ryan J Piers, Jayandra J Himali, Alexa S Beiser, Kendra L Davis-Plourde, Jane S Saczynski, David D McManus, Emelia J Enjamin, Rhoda Au
BACKGROUND: There is a paucity of longitudinal research investigating the relations between AF and domain-specific cognitive performance. OBJECTIVE: Our study investigated the association between AF and cognitive performance cross-sectionally and longitudinally. METHODS: Eligible participants were dementia- and stroke-free at the time of baseline neuropsychological (NP) assessment, and underwent at least one additional NP assessment with a one year or greater inter-test interval...
September 21, 2017: Heart Rhythm: the Official Journal of the Heart Rhythm Society
https://www.readbyqxmd.com/read/28943155/is-bone-density-associated-with-intervertebral-disc-pressure-in-healthy-and-degenerated-discs
#7
Paul M Fein, Alexander DelMonaco, Timothy M Jackman, Cameron Curtiss, Ali Guermazi, Glenn D Barest, Elise F Morgan
The coupling of the intervertebral disc (IVD) and vertebra as a biomechanical unit suggests that changes in the distribution of pressure within the IVD (intradiscal pressure, IDP) as a result of disc degeneration can influence the distribution of bone density within the vertebra, and vice versa. The goal of this study was to assess the correspondence between IDP and bone density in the adjacent vertebrae, with emphasis on how this correspondence differs between healthy and degenerated IVDs. Bone density of the endplates and subchondral bone in regions adjacent to the anterior and posterior annulus fibrosus (aAF and pAF, respectively) and nucleus pulposus (NP) was measured via quantitative computed tomography (QCT) in 61 spine segments (T7-9, T9-11, T10-12; 71±14years)...
September 4, 2017: Journal of Biomechanics
https://www.readbyqxmd.com/read/28942283/edaravone-ameliorates-compression-induced-damage-in-rat-nucleus-pulposus-cells
#8
Hui Lin, Xuan Ma, Bai-Chuan Wang, Lei Zhao, Jian-Xiang Liu, Fei-Fei Pu, Yi-Qiang Hu, Hong-Zhi Hu, Zeng-Wu Shao
AIMS: Edaravone is a strong free radical scavenger most used for treating acute ischemic stroke. In this study we investigated the protective effects and underlying mechanisms of edaravone on compression-induced damage in rat nucleus pulposus (NP) cells. MATERIALS AND METHODS: Cell viability was determined using MTT assay methods. NP cell apoptosis was measured by Hoechst 33,258 staining and Annexin V/PI double staining. Intracellular reactive oxygen species (ROS), mitochondrial membrane potential (MMP), and intracellular calcium ([Ca(2+)]i) were determined by fluorescent probes DCFH-DA, JC-1 and Fluo-3/AM, respectively...
September 20, 2017: Life Sciences
https://www.readbyqxmd.com/read/28942169/effect-of-nanoparticles-size-and-polyelectrolyte-on-nanoparticles-aggregation-in-a-cellulose-fibrous-matrix
#9
Vikram Singh Raghuwanshi, Uthpala Manavi Garusinghe, Jan Ilavsky, Warren J Batchelor, Gil Garnier
Controlling nanoparticles (NPs) aggregation in cellulose/NPs composites allows to optimise NPs driven properties and their applications. Polyelectrolytes are used to control NPs aggregation and their retention within the fibrous matrix. Here, we aim at evaluating how a polyelectrolyte (Cationic Polyacrylamide; CPAM, molecular weight: 13MDa, charge: 50%, Radius of gyration: 30-36nm) adsorbs and re-conforms onto the surface of silica(SiO2) NPs differing in diameter (8, 22 and 74nm) and to investigate the respective NPs aggregation in cellulose matrices...
September 18, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28942142/transplantation-of-dedifferentiation-fat-cells-promotes-intervertebral-disc-regeneration-in-a-rat-intervertebral-disc-degeneration-model
#10
Enshi Nakayama, Taro Matsumoto, Tomohiko Kazama, Koichiro Kano, Yasuaki Tokuhashi
Our group has reported that mature adipocyte-derived dedifferentiated fat (DFAT) cells show multilineage differentiation potential similar to that observed in mesenchymal stem cells. In the present study, we examined whether DFAT cell transplantation could contribute to intervertebral disc regeneration using a rat intervertebral disc degeneration (IDD) model. The IDD was created in Sprague-Dawley rats by puncturing at level of caudal intervertebral disc under fluoroscopy. One week after injury, rat DFAT cells (5 × 10(4), DFAT group, n = 13) or phosphate-buffered saline (PBS, control group, n = 13) were injected into the intervertebral disc...
September 20, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28941640/nanoparticle-impact-on-innate-immune-cell-pattern-recognition-receptors-and-inflammasomes-activation
#11
REVIEW
Ana Luísa Silva, Carina Peres, João Conniot, Ana I Matos, Liane Moura, Bárbara Carreira, Vanessa Sainz, Anna Scomparin, Ronit Satchi-Fainaro, Véronique Préat, Helena F Florindo
Nanotechnology-based strategies can dramatically impact the treatment, prevention and diagnosis of a wide range of diseases. Despite the unprecedented success achieved with the use of nanomaterials to address unmet biomedical needs and their particular suitability for the effective application of a personalized medicine, the clinical translation of those nanoparticulate systems has still been impaired by the limited understanding on their interaction with complex biological systems. As a result, unexpected effects due to unpredicted interactions at biomaterial and biological interfaces have been underlying the biosafety concerns raised by the use of nanomaterials...
September 20, 2017: Seminars in Immunology
https://www.readbyqxmd.com/read/28940949/ultrafast-flame-annealing-of-tio2-paste-for-fabricating-dye-sensitized-and-perovskite-solar-cells-with-enhanced-efficiency
#12
Jung Kyu Kim, Sung Uk Chai, Yoonjun Cho, Lili Cai, Sung June Kim, Sangwook Park, Jong Hyeok Park, Xiaolin Zheng
Mesoporous TiO2 nanoparticle (NP) films are broadly used as electrodes in photoelectrochemical cells, dye-sensitized solar cells (DSSCs), and perovskite solar cells (PSCs). State-of-the-art mesoporous TiO2 NP films for these solar cells are fabricated by annealing TiO2 paste-coated fluorine-doped tin oxide glass in a box furnace at 500 °C for ≈30 min. Here, the use of a nontraditional reactor, i.e., flame, is reported for the high throughput and ultrafast annealing of TiO2 paste (≈1 min). This flame-annealing method, compared to conventional furnace annealing, exhibits three distinct benefits...
September 20, 2017: Small
https://www.readbyqxmd.com/read/28940640/bicarbonate-recycling-by-hif-1-dependent-carbonic-anhydrase-isoforms-9-and-12-is-critical-in-maintaining-intracellular-ph-and-viability-of-nucleus-pulposus-cells
#13
Elizabeth S Silagi, Zachary R Schoepflin, Erin L Seifert, Christophe Merceron, Ernestina Schipani, Irving M Shapiro, Makarand V Risbud
Intervertebral disc degeneration is a ubiquitous condition closely linked to chronic low-back pain. The health of the avascular nucleus pulposus (NP) plays a crucial role in the development of this pathology. We tested the hypothesis that a network comprising HIF-1α, Carbonic anhydrase (CA) 9 and 12 isoforms, and sodium-coupled bicarbonate cotransporters (NBCs) buffer intracellular pH through coordinated bicarbonate recycling. Contrary to the current understanding of NP cell metabolism, analysis of metabolic-flux data from Seahorse XF analyzer showed that CO2 hydration contributes a significant source of extracellular proton production in NP cells, with a smaller input from glycolysis...
September 23, 2017: Journal of Bone and Mineral Research: the Official Journal of the American Society for Bone and Mineral Research
https://www.readbyqxmd.com/read/28939840/tuneable-pressure-effects-in-graphene-oxide-layers
#14
Yusuke Sekimoto, Ryo Ohtani, Masaaki Nakamura, Michio Koinuma, Leonard F Lindoy, Shinya Hayami
Tuneable pressure effects associated with changing interlayer distances in two-dimensional graphene oxide (GO)/reduced GO (rGO) layers are demonstrated through monitoring the changes in the spin-crossover (SCO) temperature (T 1/2) of [Fe(Htrz)2(trz)](BF4) nanoparticles (NPs) incorporated in the interlayer spaces of the GO/rGO layers. The interlayer separation along the GO to GO/rGO-NP composites to rGO series decreases smoothly from 9.00 Å (for GO) to 3.50 Å (for rGO) as the temperature employed for the thermal reduction treatments of the GO-NP composites is increased...
September 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28939108/differential-response-to-doxorubicin-in-breast-cancer-subtypes-simulated-by-a-microfluidic-tumor-model
#15
Altug Ozcelikkale, Kyeonggon Shin, Victoria Noe-Kim, Bennett D Elzey, Zizheng Dong, Jian-Ting Zhang, Kwangmeyung Kim, Ick Chan Kwon, Kinam Park, Bumsoo Han
Successful drug delivery and overcoming drug resistance are the primary clinical challenges for management and treatment of cancer. The ability to rapidly screen drugs and delivery systems within physiologically relevant environments is critically important; yet is currently limited due to lack of appropriate tumor models. To address this problem, we developed the Tumor-microenvironment-on-chip (T-MOC), a new microfluidic tumor model simulating the interstitial flow, plasma clearance, and transport of the drug within the tumor...
September 19, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28938600/application-of-dual-targeting-drug-delivery-system-for-the-improvement-of-anti-glioma-efficacy-of-doxorubicin
#16
Zhenliang Sun, Xuebing Yan, YiBo Liu, Linsheng Huang, Cheng Kong, Xiao Qu, Man Wang, Renyuan Gao, Huanlong Qin
Chemotherapy of glioma is always hampered by the unsatisfactory tumor accumulation of drugs, of which the most noticeable obstacle is the limited drug permeability from vessels into tumor inner. In the present study, we developed a novel nanocarrier for the delivery of doxorubicin to brain tumor. Such novel drug delivery system was mainly composed of a tumor homing peptide and DOX-loaded PLA nanoparticles (AP1-NP-DOX). CRKRLDRNC peptide, named as AP1, was a newly glioma affinity peptide which could specifically binds to interleukin-4 receptor (IL-4R), highly expressing on both glioma cells and angiogenesis...
August 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/28938440/sex-differences-in-the-hormonal-and-metabolic-response-to-dietary-protein-dilution
#17
Karlton R Larson, Kimberly A Russo, Yanbin Fang, Niloufar Mohajerani, Michael L Goodson, Karen K Ryan
Consumption of a low-protein, high-carbohydrate diet induces a striking increase in circulating fibroblast growth factor-21 (FGF21), which is associated with improved cardiometabolic health and increased longevity. Increased lifespan during this dietary protein 'dilution' has been explained by resource-mediated trade-offs between reproduction and survival, such that fecundity is optimized at a greater relative intake of proteins:carbohydrates. The magnitude of this trade-off is thought to be sex-dependent. Here, we tested the hypothesis that metabolic responses to dietary protein dilution are likewise dependent on sex...
August 9, 2017: Endocrinology
https://www.readbyqxmd.com/read/28938207/zinc-oxide-nanoparticles-in-predicted-environmentally-relevant-concentrations-leading-to-behavioral-impairments-in-male-swiss-mice
#18
Joyce Moreira de Souza, Bruna de Oliveira Mendes, Abraão Tiago Batista Guimarães, Aline Sueli de Lima Rodrigues, Thales Quintão Chagas, Thiago Lopes Rocha, Guilherme Malafaia
Although the potential neurotoxic effects from the exposure to zinc oxide nanoparticles (ZnO NPs) on humans and on experimental models have been reported in previous studies, the effects from the exposure to environmentally relevant concentrations of them remain unclear. Thus, the aim of the present study is to investigate the effects from the exposure to environmentally relevant concentrations of ZnO NPs on the behavior of male Swiss mice. The animals were daily exposed to environmentally relevant concentrations of ZnO NPs (5...
September 19, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28937298/probing-the-toxicity-of-nanoparticles-a-unified-in-silico-machine-learning-model-based-on-perturbation-theory
#19
Riccardo Concu, Valeria V Kleandrova, Alejandro Speck-Planche, M Natália D S Cordeiro
Nanoparticles (NPs) are part of our daily life, having a wide range of applications in engineering, physics, chemistry, and biomedicine. However, there are serious concerns regarding the harmful effects that NPs can cause to the different biological systems and their ecosystems. Toxicity testing is an essential step for assessing the potential risks of the NPs, but the experimental assays are often very expensive and usually too slow to flag the number of NPs that may cause adverse effects. In silico models centered on quantitative structure-activity/toxicity relationships (QSAR/QSTR) are alternative tools that have become valuable supports to risk assessment, rationalizing the search for safer NPs...
September 22, 2017: Nanotoxicology
https://www.readbyqxmd.com/read/28937168/supracolloidal-chains-of-patchy-micelles-of-diblock-copolymers-with-in-situ-synthesized-nanoparticles
#20
Sukwoo Jang, Kyungtae Kim, Jonghyuk Jeon, Donghwi Kang, Byeong-Hyeok Sohn
Supracolloidal chains of diblock copolymer micelles were functionalized with gold and silver nanoparticles (NPs). Both NPs were independently synthesized in situ in the core of spherical micelles which were then converted to patchy micelles. With these patchy micelles as colloidal monomers, supracolloidal chains were polymerized by combining the patches of neighboring micelles. Since all micelles contained NPs, NPs were incorporated in every repeat unit of chains. In addition, a single gold NP was synthesized in the micellar core in contrast to several silver NPs so that we differentiated the chains with Au NPs from those with Ag NPs by the number of NPs in the repeat unit as well as by plasmonic bands in UV-Vis spectra...
September 22, 2017: Soft Matter
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