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https://www.readbyqxmd.com/read/28731278/knockdown-of-long-non-coding-rna-meg3-protects-h9c2-cells-from-hypoxia-induced-injury-by-targeting-microrna-183
#1
Licheng Gong, Haiming Xu, Hong Chang, Yaliang Tong, Tao Zhang, Gongliang Guo
Acute myocardial infarction (AMI) occurs when blood supply to the heart is diminished (ischemia) for long time, and ischemia is primarily caused due to hypoxia. This study evaluated the effects of long non-coding RNA maternally expressed gene 3 (MEG3) on hypoxic rat cardiomyocyte-drived H9c2 cells. Hypoxic injury was confirmed by alterations of cell viability, migration, invasion, apoptosis and hypoxia-inducible factor 1α (HIF-1α) expression. MEG3 level in hypoxic cells and effects of its knockdown on hypoxic cells were assessed...
July 21, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28723640/trim22-confers-poor-prognosis-and-promotes-epithelial-mesenchymal-transition-through-regulation-of-akt-gsk3%C3%AE-%C3%AE-catenin-signaling-in-non-small-cell-lung-cancer
#2
Li Liu, Xiao-Ming Zhou, Fang-Fei Yang, Yuan Miao, Yan Yin, Xue-Jun Hu, Gang Hou, Qiu-Yue Wang, Jian Kang
Expression pattern and biological roles of TRIM22 remains unknown in most human cancers. The present study aims to discover its clinical significance and function in human non-small cell lung cancer (NSCLC). Immunohistochemistry was used to examine TRIM22 expression in 126 cases of NSCLC specimens. TRIM22 protein was upregulated in 70/126 (55.6%) non-small cell lung cancer tissues compared with normal lung tissue. TRIM22 overexpression was associated with advanced TNM stage, positive nodal metastasis and poor prognosis...
July 1, 2017: Oncotarget
https://www.readbyqxmd.com/read/28718667/hydroxytyrosol-induces-apoptosis-and-cell-cycle-arrest-and-suppresses-multiple-oncogenic-signaling-pathways-in-prostate-cancer-cells
#3
Haseeb Zubair, Arun Bhardwaj, Aamir Ahmad, Sanjeev Kumar Srivastava, Mohammad Aslam Khan, Girijesh Kumar Patel, Seema Singh, Ajay Partap Singh
SCOPE: Hydroxytyrosol (HT), a polyphenol from olives, is a potential anticancer agent. This study was designed to evaluate the anticancer activity of HT against prostate cancer cells, and the mechanism thereof. METHODS AND RESULTS: Treatment of LNCaP and C4-2 prostate cancer cells with HT resulted in a dose-dependent inhibition of proliferation. This was in contrast to HT's ineffectiveness against normal prostate epithelial cells RWPE1 and PWLE2, suggesting cancer-cell-specific effect...
July 18, 2017: Nutrition and Cancer
https://www.readbyqxmd.com/read/28718046/complete-genome-sequencing-and-characterization-revealed-a-recombinant-subgroup-b-isolate-of-avian-leukosis-virus-with-a-subgroup-j-like-u3-region
#4
Peikun Wang, Yongli Yang, Lulu Lin, Haijuan Li, Ping Wei
One natural recombinant subgroup B avian leukosis virus (ALV) with a subgroup J-like U3 region was isolated from commercial native chickens that experienced disease in 2014 and named GX14FF03. GX14FF03 was isolated by DF-1 cell culture and then identified with ELISA detection of avian leukosis virus p27 group-specific antigen, the detection of subtype specific PCR, and indirect immunofluorescence assay with ALV-B-specific monoclonal antibody. Its complete proviral genome was sequenced and compared with the reference strains of ALVs and found that the gag and pol were relatively conservative...
July 17, 2017: Virus Genes
https://www.readbyqxmd.com/read/28718045/full-length-genome-sequence-analysis-of-four-subgroup-j-avian-leukosis-virus-strains-isolated-from-chickens-with-clinical-hemangioma
#5
Lulu Lin, Peikun Wang, Yongli Yang, Haijuan Li, Teng Huang, Ping Wei
Since 2014, cases of hemangioma associated with avian leukosis virus subgroup J (ALV-J) have been emerging in commercial chickens in Guangxi. In this study, four strains of the subgroup J avian leukosis virus (ALV-J), named GX14HG01, GX14HG04, GX14LT07, and GX14ZS14, were isolated from chickens with clinical hemangioma in 2014 by DF-1 cell culture and then identified with ELISA detection of ALV group specific antigen p27, the detection of subtype specific PCR and indirect immunofluorescence assay (IFA) with ALV-J specific monoclonal antibody...
July 17, 2017: Virus Genes
https://www.readbyqxmd.com/read/28717191/mln4924-pevonedistat-a-protein-neddylation-inhibitor-suppresses-proliferation-and-migration-of-human-clear-cell-renal-cell-carcinoma
#6
Shuai Tong, Yang Si, Hefen Yu, Lingqiang Zhang, Ping Xie, Wenguo Jiang
Neddylation is a post-translational protein modification associated with cancer development. MLN4924 is a neddylation inhibitor currently under investigation in multiple phase I studies on various malignancies, and its clincal name is Pevonedistat. It has been documented that MLN4924 blocks Cullins neddylation and inactivates CRLs and, in turn, triggers cell-cycle arrest, apoptosis, senescence and autophagy in many cancer cells. In this study, we investigated the anti-tumor effect of MLN4924 in human clear cell renal carcinoma (ccRCC)...
July 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28716056/the-inhibitory-effect-of-isoliquiritigenin-on-the-proliferation-of-human-arterial-smooth-muscle-cell
#7
Tianbao Chen, Shaoxiong Deng, Rong Lin
BACKGROUND: Isoliquiritigenin (ISL) has various biological activities including as antioxidant and an inhibitor of PI3K/AKT signaling pathway. However, both oxidative stress and activated PI3K/AKT signaling contribute to the aberrant proliferation of vascular smooth muscle cells (VSMCs). This study is aimed to explore the effect of ISL on the proliferation of human arterial smooth muscle cells (HASMCs) and to investigate the underlying mechanisms. METHODS: BrdU incorporation, cell cycle and reactive oxygen species (ROS) in normal or ISL treated HASMCs were analyzed by flow cytometry...
July 17, 2017: BMC Pharmacology & Toxicology
https://www.readbyqxmd.com/read/28713980/mirna%C3%A2-1284-inhibits-cell-growth-and-induces-apoptosis-of-lung-cancer-cells
#8
Jie Li, Hairong Jin, Hua Yu, Bin Wang, Jian Tang
Lung cancer is the most common cancer worldwide, and morbidity and mortality associated with lung cancer has been increasing annually in recent decades. MicroRNAs (miRNAs), which are short non‑coding RNA sequences that are involved in the regulation of gene expression, have been previously demonstrated to be key regulators in cancer. The present study aimed to clarify the role of miRNA (miR)‑1284 in lung cancer. A549 lung carcinoma cells were transfected with miR‑1284 mimic or miR‑1284 inhibitor using Lipofectamine 2000...
July 12, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28713923/the-differential-regulation-of-micrornas-is-associated-with-oral-cancer
#9
Shih-Chang Tsai, Sheng-Fong Huang, Jo-Hua Chiang, Yen-Fu Chen, Chia-Chang Huang, Ming-Hsui Tsai, Fuu-Jen Tsai, Ming-Ching Kao, Jai-Sing Yang
Oral squamous cell carcinoma (OSCC), is the most frequently occurring malignant head and neck tumor, generally it exhibits a poor prognosis, and metastasis is the main cause of death in these cancer patients. The discovery of reliable prognostic indicators for tumors progression would greatly improve clinical treatments. MicroRNAs (miRNAs) play a critical role in the degradation of mRNA and the inhibition of protein synthesis. The miRNAs function either as tumor suppressors or as oncogenes in tumorigenesis, and little is known about the clinical significance of miRNA expression profiles in oral cancers...
July 12, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28711931/apc-c-is-essential-for-hematopoiesis-and-impaired-in-aplastic-anemia
#10
Jia Wang, Min-Zhi Yin, Ke-Wen Zhao, Fang Ke, Wen-Jie Jin, Xiao-Lin Guo, Tian-Hui Liu, Xiao-Ye Liu, Hao Gu, Xiao-Min Yu, Zhen Li, Li-Li Mu, Deng-Li Hong, Jing Chen, Guo-Qiang Chen
Anaphase promoting complex/cyclosome (APC/C) is essential for cell cycle progression. Recently, its non-mitotic functions were also reported but less studied in several tissues including hematopoietic cells. Here, we developed an inducible Anapc2 (a core subunit of APC/C) knockout mice. The animals displayed a fatal bone marrow failure within 7 days after knockout induction. Their hematopoietic stem and progenitor cells (HSPCs) demonstrated a sharp decline and could form little colony. Further, the results of BrdU label-retaining cell assay showed that the dormant HPSCs lost rapidly...
June 28, 2017: Oncotarget
https://www.readbyqxmd.com/read/28708871/ursodeoxycholic-acid-inhibits-the-proliferation-of-colon-cancer-cells-by-regulating-oxidative-stress-and-cancer-stem-like-cell-growth
#11
Eun-Kyung Kim, Jae Hee Cho, EuiJoo Kim, Yoon Jae Kim
INTRODUCTION: The regulation of reactive oxygen species (ROS) exists as a therapeutic target for cancer treatments. Previous studies have shown that ursodeoxycholic acid (UDCA) suppresses the proliferation of colon cancer cells. The aim of this study was to evaluate the effect of UDCA upon the proliferation of colon cancer cells as a direct result of the regulation of ROS. METHOD: Colon cancer cell lines (HT29 and HCT116) were treated with UDCA. The total number of cells and the number of dead cells were determined using cell counters...
2017: PloS One
https://www.readbyqxmd.com/read/28704759/novel-pyrazolopyrimidines-synthesis-in%C3%A2-vitro-cytotoxic-activity-and-mechanistic-investigation
#12
Ghaneya S Hassan, Doaa E Abdel Rahman, Yassin M Nissan, Esraa A Abdelmajeed, Tamer M Abdelghany
A series of novel pyrazolo[3,4-d]pyrimidines bearing benzenesulfonamide moiety 5a-f, 6 and 7 were synthesized. Cytotoxic screening was conducted against MCF-7 and HepG2. 6-(4-Methoxyphenyl)-4-oxopyrazolopyrimidine derivative 5e and 4-imino-6-oxopyrazolopyrimidine derivative 6 revealed potent cytotoxic activity with IC50 1.4 μM (MCF-7) and 0.4 μM (HepG2), respectively compared to that of doxorubicin, (IC50 = 1.02 μM and 0.9 μM, respectively). Compounds 5e and 6 were subjected to cell cycle analysis and apoptosis assay after 24 h and 48 h treatment...
July 3, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28702842/essential-role-of-sh3gl1-in-interleukin-6-il-6-and-vascular-endothelial-growth-factor-vegf-triggered-p130-cas-mediated-proliferation-and-migration-of-osteosarcoma-cells
#13
En-Qi Li, Jin-Li Zhang
We recently demonstrated that interleukin-6 (IL-6)- and vascular endothelial growth factor (VEGF)-induced osteosarcoma (OS) cell proliferation and migration are parallel to significant increased expression of SH3GL1 and the phosphorylation level of P130(cas). The expression level of SH3GL1 was widely upregulated in human OS tissues, and their overexpression was significantly correlated with more aggressive clinicopathological features. Conversely, depletion of SH3GL1 by adenovirus shRNA abrogates P130(cas) phosphorylation and IL-6- and VEGF-induced OS cell proliferation and migration...
July 12, 2017: Human Cell
https://www.readbyqxmd.com/read/28701864/induction-of-forkhead-class-box-o3a-and-apoptosis-by-a-standardized-ginsenoside-formulation-kg-135-is-potentiated-by-autophagy-blockade-in-a549-human-lung-cancer-cells
#14
Chih-Jung Yao, Jyh-Ming Chow, Shuang-En Chuang, Chia-Lun Chang, Ming-De Yan, Hsin-Lun Lee, I-Chun Lai, Pei-Chun Lin, Gi-Ming Lai
BACKGROUND: KG-135, a standardized formulation enriched with Rk1, Rg3, and Rg5 ginsenosides, has been shown to inhibit various types of cancer cells; however, the underlying mechanisms are not fully understood. In this study, we explored its effects in A549 human lung cancer cells to investigate the induction of Forkhead Class box O3a (FOXO3a) and autophagy. METHODS: Cell viability was determined by sulforhodamine B staining. Apoptosis and cell cycle distribution were analyzed using flow cytometry...
July 2017: Journal of Ginseng Research
https://www.readbyqxmd.com/read/28701359/oxldl-induced-endothelial-proliferation-via-rho-rock-akt-p27-kip1-signaling-prevention-by-cholesterol-loading
#15
Chongxu Zhang, Crystal Adamos, Myung-Jin Oh, Jugajyoti Baruah, Manuela A A Ayee, Dolly Mehta, Kishore K Wary, Irena Levitan
Oxidized modifications of LDL (oxLDL) are known to play a major role in endothelial dysfunction and the development of atherosclerosis. In this study, we provide comparative analysis of the effects of two major types of oxLDL, copper oxidized LDL (Cu(2+)-oxLDL) and Lipoxygenase oxidized LDL (LPO-oxLDL) on proliferation of Human Aortic Endothelial Cells (HAECs). We show that Cu(2+)-oxLDL enhances HAECs proliferation in a dose and oxidation-dependent manner. Similarly, LPO-oxLDL also has a positive effect on HAECs proliferation...
July 12, 2017: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/28701314/cux1-promotes-cell-proliferation-and-polycystic-kidney-disease-progression-in-an-adpkd-mouse-model
#16
Binu Porath, Safia Livingston, Erica L Andres, Alexandra M Petrie, Joshua C Wright, Anna E Woo, Carol G Carlton, Richard Baybutt, Gregory B Vanden Heuvel
Autosomal Dominant Polycystic Kidney Disease (ADPKD) is one of the most common monogenic hereditary disorders in humans characterized by fluid-filled cysts, primarily in the kidneys. Cux1, a cell cycle regulatory gene highly expressed during kidney development, is elevated in the cyst-lining cells of Pkd1 mutant mice, and in human ADPKD cells. However, forced expression of Cux1 is insufficient to induce cystic disease in transgenic mice, or to induce rapid cyst formation after cilia disruption in the kidneys of adult mice...
July 12, 2017: American Journal of Physiology. Renal Physiology
https://www.readbyqxmd.com/read/28699905/fkbp51-decreases-cell-proliferation-and-increases-progestin-sensitivity-of-human-endometrial-adenocarcinomas-by-inhibiting-akt
#17
Jing Dong, Yulian Jiao, Wenli Mu, Bingru Lu, Muyun Wei, Linying Sun, Shengnan Hu, Bin Cui, Xiaowen Liu, Zijiang Chen, Yueran Zhao
In this study, we investigated the role of FK506 binding protein 51 (FKBP51) in human endometrial adenocarcinoma progression. Immunohistochemical analysis showed decreased FKBP51 expression in endometrial adenocarcinoma tissues. Moreover, higher FKBP51 expression was observed in the normal secretory phase than in proliferative-phase endometrial tissues. FKBP51-shRNA transfected KLE cells showed high Ser473-phospho Akt with decreased p21 and p27 levels, which promoted S-G2/M phase cell cycle progression and proliferation...
June 30, 2017: Oncotarget
https://www.readbyqxmd.com/read/28698047/identification-of-a-novel-linear-b-cell-epitope-in-the-p27-of-avian-leukosis-virus
#18
Wiaam O A Khairy, Lin Wang, Xue Tian, Jianqiang Ye, Kun Qian, Hongxia Shao, Aijian Qin
Avian leukosis virus (ALV) is an avian oncogenic retrovirus that can induce virus-associated neoplasia and causes great economic loss in poultry industry. It is known that the capsid antigen p27 is the group-specific antigen that is highly conserved among all ALV subgroups, and is the most abundant immunogenic viral protein. In the present study, five overlapping fragments (GST- p27-F1/2, GST- p27-F2-1/2/3) of ALV-p27 were subjected to Western blotting analysis using a monoclonal antibody (5D3) against ALV-p27 to identify the epitope...
July 8, 2017: Virus Research
https://www.readbyqxmd.com/read/28695384/carbonic-anhydrase-inhibitors-induce-developmental-toxicity-during-zebrafish-embryogenesis-especially-in-the-inner-ear
#19
Hiroko Matsumoto, Shoko Fujiwara, Hisako Miyagi, Nobuhiro Nakamura, Yasuhiro Shiga, Toshihiro Ohta, Mikio Tsuzuki
In vertebrates, carbonic anhydrases (CAs) play important roles in ion transport and pH regulation in many organs, including the eyes, kidneys, central nervous system, and inner ear. In aquatic organisms, the enzyme is inhibited by various chemicals present in the environment, such as heavy metals, pesticides, and pharmaceuticals. In this study, the effects of CA inhibitors, i.e., sulfonamides [ethoxyzolamide (EZA), acetazolamide (AZA), and dorzolamide (DZA)], on zebrafish embryogenesis were investigated. In embryos treated with the sulfonamides, abnormal development, such as smaller otoliths, an enlarged heart, an irregular pectoral fin, and aberrant swimming behavior, was observed...
July 10, 2017: Marine Biotechnology
https://www.readbyqxmd.com/read/28689658/conformational-landscape-of-the-p28-bound-human-proteasome-regulatory-particle
#20
Ying Lu, Jiayi Wu, Yuanchen Dong, Shuobing Chen, Shuangwu Sun, Yong-Bei Ma, Qi Ouyang, Daniel Finley, Marc W Kirschner, Youdong Mao
The proteasome holoenzyme is activated by its regulatory particle (RP) consisting of two subcomplexes, the lid and the base. A key event in base assembly is the formation of a heterohexameric ring of AAA-ATPases, which is guided by at least four RP assembly chaperones in mammals: PAAF1, p28/gankyrin, p27/PSMD9, and S5b. Using cryogenic electron microscopy, we analyzed the non-AAA structure of the p28-bound human RP at 4.5 Å resolution and determined seven distinct conformations of the Rpn1-p28-AAA subcomplex within the p28-bound RP at subnanometer resolutions...
July 20, 2017: Molecular Cell
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