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https://www.readbyqxmd.com/read/28211871/expression-level-is-a-key-determinant-of-e2f1-mediated-cell-fate
#1
Igor Shats, Michael Deng, Adam Davidovich, Carolyn Zhang, Jungeun S Kwon, Dinesh Manandhar, Raluca Gordân, Guang Yao, Lingchong You
The Rb/E2F network has a critical role in regulating cell cycle progression and cell fate decisions. It is dysfunctional in virtually all human cancers, because of genetic lesions that cause overexpression of activators, inactivation of repressors, or both. Paradoxically, the downstream target of this network, E2F1, is rarely strongly overexpressed in cancer. E2F1 can induce both proliferation and apoptosis but the factors governing these critical cell fate decisions remain unclear. Previous studies have focused on qualitative mechanisms such as differential cofactors, posttranslational modification or state of other signaling pathways as modifiers of the cell fate decisions downstream of E2F1 activation...
February 17, 2017: Cell Death and Differentiation
https://www.readbyqxmd.com/read/28203057/kelussia-odoratissima-mozaff-activates-intrinsic-pathway-of-apoptosis-in-breast-cancer-cells-associated-with-s-phase-cell-cycle-arrest-via-involvement-of-p21-p27-in-vitro-and-in-vivo
#2
Hamed Karimian, Aditya Arya, Mehran Fadaeinasab, Mahboubeh Razavi, Maryam Hajrezaei, Ataul Karim Khan, Hapipah Mohd Ali, Mahmood Ameen Abdulla, Mohamad Ibrahim Noordin
BACKGROUND: The aim of this study was to evaluate the anticancer potential of Kelussia odoratissima. Several in vitro and in vivo biological assays were applied to explore the direct effect of an extract and bioactive compound of this plant against breast cancer cells and its possible mechanism of action. MATERIALS AND METHODS: K. odoratissima methanol extract (KME) was prepared, and MTT assay was used to evaluate the cytotoxicity. To identify the cytotoxic compound, a bioassay-guided investigation was performed on methanol extract...
2017: Drug Design, Development and Therapy
https://www.readbyqxmd.com/read/28193232/peg10-overexpression-induced-by-e2f-1-promotes-cell-proliferation-migration-and-invasion-in-pancreatic-cancer
#3
Yun-Peng Peng, Yi Zhu, Ling-Di Yin, Jing-Jing Zhang, Ji-Shu Wei, Xian Liu, Xin-Chun Liu, Wen-Tao Gao, Kui-Rong Jiang, Yi Miao
BACKGROUND: Overexpression of paternally expressed gene-10 (PEG10) is known to promote the progression of several carcinomas, however, its role in pancreatic cancer (PC) is unknown. We investigated the expression and function of PEG10 in PC. METHODS: PEG10 expression and correlation with PC progression was assessed in cancerous tissues and paired non-cancerous tissues. Further, the role of PEG10 in PC cell progression and the underlying mechanisms were studied by using small interfering RNA (Si-RNA)...
February 13, 2017: Journal of Experimental & Clinical Cancer Research: CR
https://www.readbyqxmd.com/read/28185194/high-sensitivity-ihc-detection-of-phosphorylated-p27-kip1-in-human-tissues-using-secondary-antibody-conjugated-to-polymer-hrp
#4
Michael Grahek, Ana Ptak, Alexander E Kalyuzhny
A complex composed of goat anti-rabbit secondary antibody conjugated to a polymer coated with horseradish peroxidase (HRP) molecules was used to develop rapid and highly sensitive immunostaining protocol for the detection of phosphorylated p27/Kip1 (T157) in human tissues. This polymer-HRP complex produced much better sensitivity detection compared to conventional biotin-streptavidin-HRP chemistry. Using polymer-HRP made it possible to reduce primary antibody concentration, eliminate some incubation steps such as avidin-biotin blocking and incubation with separate biotinylated secondary antibodies, and shorten the incubation time with primary antibody...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28178760/histone-deacetylase-7-silencing-induces-apoptosis-and-autophagy-in-salivary-mucoepidermoid-carcinoma-cells
#5
Mee-Young Ahn, Jung-Hoon Yoon
BACKGROUND: The overexpression of histone deacetylases (HDACs) has been observed in many cancers and inhibition of specific HDACs has emerged as a new target for cancer therapy. We found that HDAC7 expression was selectively reduced by HDAC inhibitor apicidin in salivary mucoepidermoid carcinoma (MEC) cells. Here, we show that HDAC7 suppression has a potent anti-tumor effect in MEC cells. METHODS: HDAC7 was knocked down using HDAC7 siRNAs and cell proliferation was quantified...
February 8, 2017: Journal of Oral Pathology & Medicine
https://www.readbyqxmd.com/read/28176922/inhibition-of-skp2-sensitizes-lung-cancer-cells-to-paclitaxel
#6
Tonghai Huang, Lin Yang, Guangsuo Wang, Guanggui Ding, Bin Peng, Yuxin Wen, Zheng Wang
S-phase kinase-associated protein 2 (Skp2) is an E3 ubiquitin ligase and plays an important role in the control of cell cycle progression. Skp2 is upregulated in several cancers, including lung cancers, but the role of Skp2 in the tumorigenesis and anticancer drug resistance in human lung cancer remains to be determined. We report here that Skp2 positively regulated mitotic arrest deficient 2 (MAD2) expression and that inhibition of Skp2 sensitizes human lung cancer cells to paclitaxel. Knockdown of Skp2 by small interfering RNA (siRNA) decreased Mad2 messenger RNA (mRNA) and protein levels in A549 and NCI-H1975 cells, accompanied with upregulation of p27 but decrease of the phosphorylation of retinoblastoma (Rb)...
2017: OncoTargets and Therapy
https://www.readbyqxmd.com/read/28176351/role-of-connexin-43-in-cadmium-induced-proliferation-of-human-prostate-epithelial-cells
#7
Qingping Liu, Xiaoli Ji, Zehe Ge, Haipeng Diao, Xiuli Chang, Lihua Wang, Qing Wu
Connexins (Cxs), the subunits of gap junction channels, are involved in many physiological processes. Aberrant control of Cxs and gap junction intercellular communication may contribute to many diseases, including the promotion of cancer. Cd exposure is associated with increased risk of human prostate cancer and benign prostatic hyperplasia. The roles of Cxs in the effects of Cd on the prostate have, however, not been reported previously. In this study, the human prostate epithelial cell line RWPE-1 was exposed to Cd...
February 8, 2017: Journal of Applied Toxicology: JAT
https://www.readbyqxmd.com/read/28174135/a-system-of-recurrent-neural-networks-for-modularising-parameterising-and-dynamic-analysis-of-cell-signalling-networks
#8
S Samarasinghe, H Ling
In this paper, we show how to extend our previously proposed novel continuous time Recurrent Neural Networks (RNN) approach that retains the advantage of continuous dynamics offered by Ordinary Differential Equations (ODE) while enabling parameter estimation through adaptation, to larger signalling networks using a modular approach. Specifically, the signalling network is decomposed into several sub-models based on important temporal events in the network. Each sub-model is represented by the proposed RNN and trained using data generated from the corresponding ODE model...
February 4, 2017: Bio Systems
https://www.readbyqxmd.com/read/28168733/a-case-of-linear-basal-cell-carcinoma-evaluation-of-proliferative-activity-by-immunohistochemical-staining-of-pctaire1-and-p27
#9
Y Yamaguchi, T Yanagi, K Imafuku, S Kitamura, H Hata, H Nishihara, H Shimizu
Linear basal cell carcinoma (LBCC) is a unique morphological variant of basal cell carcinoma (BCC)(1-3) , and the mechanism behind the linear form has yet to be fully elucidated. To date, we have investigated the functions of p27 (Kip1) and PCTAIRE1 (also known as PCTK1, Cdk16) in tumorigenesis.(4, 5) PCTAIRE1 is a member of the PCTAIRE family, which is related to the Cdk family, and it phosphorylates p27, leading to ubiquitination/degradation of this tumor suppressor.(6, 7) An inverse correlation between p27 and PCTAIRE1 expressions has been observed in malignant tumors...
February 6, 2017: Journal of the European Academy of Dermatology and Venereology: JEADV
https://www.readbyqxmd.com/read/28160664/identification-of-a-maize-chlorotic-dwarf-virus-silencing-suppressor-protein
#10
Lucy R Stewart, Sridhar Jarugula, Yujing Zhao, Feng Qu, DeeMarie Marty
Maize chlorotic dwarf virus (MCDV), a member of the genus Waikavirus, family Secoviridae, has a 11784 nt (+)ssRNA genome that encodes a 389kDa proteolytically processed polyprotein. We show that the N-terminal 78kDa polyprotein (R78) of MCDV acts as a suppressor of RNA silencing in a well-established assay system. We further demonstrate that R78 is cleaved by the viral 3C-like protease into 51 and 27kDa proteins (p51 and p27), and that p51 is responsible for silencing suppressor activity. Silencing suppressor activity of R78 is conserved in three divergent MCDV strains (MCDV-Severe, MCDV-M1, and MCDV-Tennessee), as well as the waikavirus Bellflower vein chlorosis virus, but was not detected for orthologous protein of Rice tungro spherical virus (RTSV-A) or the similarly-positioned protein from the sequivirus Parsnip yellow fleck virus (PYFV)...
February 1, 2017: Virology
https://www.readbyqxmd.com/read/28157724/orchestration-of-late-events-in-erythropoiesis-by-klf1-eklf
#11
Merlin Nithya Gnanapragasam, James J Bieker
PURPOSE OF REVIEW: Transcriptional regulators provide the molecular and biochemical basis for the cell specific properties and characteristics that follow from their central role in establishing tissue-restricted expression. Precise and sequential control of terminal cell divisions, nuclear condensation, and enucleation are defining characteristics within erythropoietic differentiation. This review is focused on KLF1, a central global regulator of this process. RECENT FINDINGS: Studies in the past year have brought a number of proteins that are targets of KLF1 regulation into focus with respect to their roles in terminal erythroid differentiation...
February 2, 2017: Current Opinion in Hematology
https://www.readbyqxmd.com/read/28157137/extracellular-polyamines-induced-proliferation-and-migration-of-cancer-cells-by-odc-ssat-and-akt1-mediated-pathway
#12
Fujun Dai, Wubei Yu, Jia Song, Qian Li, Chaojie Wang, Songqiang Xie
High levels of polyamines were observed and were related to a poor prognosis in cancer patients. However, the mechanism is not obvious. The aim of this study is to mimic the extracellular polyamines in a tumor microenviroment and to explore the role of extracellular polyamines in the proliferation and migration of cancer cells. Three different concentrations of polyamines composed of putrescine, spermidine, and spermine were used. Colony formation assay, wound healing assay, and transwell migration assay were performed...
February 2, 2017: Anti-cancer Drugs
https://www.readbyqxmd.com/read/28154139/harnessing-host-ros-generating-machinery-for-the-robust-genome-replication-of-a-plant-rna-virus
#13
Kiwamu Hyodo, Kenji Hashimoto, Kazuyuki Kuchitsu, Nobuhiro Suzuki, Tetsuro Okuno
As sessile organisms, plants have to accommodate to rapid changes in their surrounding environment. Reactive oxygen species (ROS) act as signaling molecules to transduce biotic and abiotic stimuli into plant stress adaptations. It is established that a respiratory burst oxidase homolog B of Nicotiana benthamiana (NbRBOHB) produces ROS in response to microbe-associated molecular patterns to inhibit pathogen infection. Plant viruses are also known as causative agents of ROS induction in infected plants; however, the function of ROS in plant-virus interactions remains obscure...
February 1, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28141839/dasatinib-inhibits-c-src-phosphorylation-and-prevents-the-proliferation-of-triple-negative-breast-cancer-tnbc-cells-which-overexpress-syndecan-binding-protein-sdcbp
#14
Xiao-Long Qian, Jun Zhang, Pei-Ze Li, Rong-Gang Lang, Wei-Dong Li, Hui Sun, Fang-Fang Liu, Xiao-Jing Guo, Feng Gu, Li Fu
Triple negative breast cancer (TNBC) progresses rapidly but lacks effective targeted therapies. Our previous study showed that downregulating syndecan-binding protein (SDCBP) in TNBC inhibits the proliferation of TNBC cells. Dasatinib is a new small-molecule inhibitor of c-src phosphorylation. The aim of this study was to investigate if SDCBP is a potential marker to indicate whether a TNBC is suitable for dasatinib therapy. This study applied co-immunoprecipitation to identify the interaction between SDCBP and c-src in TNBC cell lines...
2017: PloS One
https://www.readbyqxmd.com/read/28139736/young-bone-marrow-sca-1-stem-cells-rejuvenate-the-aged-heart-and-improve-function-after-injury-through-pdgfr%C3%AE-akt-pathway
#15
Shu-Hong Li, Lu Sun, Lei Yang, Jiao Li, Zhengbo Shao, Guo-Qing Du, Jun Wu, Richard D Weisel, Ren-Ke Li
Bone marrow (BM) reconstitution with young BM cells in aged recipients restores the functionality of cardiac resident BM-derived progenitors. This study investigated the cell type primarily responsible for this effect. We reconstituted old mice with BM cells from young or old mice and found that the number of stem cell antigen 1 (Sca-1) cells homing to the heart was significantly greater in young than old chimeras. We then reconstituted old mice with young BM Sca-1(+) or Sca-1(-) cells. We found that Sca-1 cells repopulated the recipient BM and homed to the heart...
January 31, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28134947/identification-of-terpenoids-from-rubus-corchorifolius-l-f-leaves-and-their-anti-proliferative-effects-on-human-cancer-cells
#16
Xuexiang Chen, Zili Gao, Mingyue Song, Wen Ouyang, Xian Wu, Yunjiao Chen, Liping Zhou, Dixon William, Xiaokun Cai, Yong Cao, Shuangde Zhou, Zhonghai Tang, Hang Xiao
The leaves of Rubus corchorifolius L. f. have been consumed as a herbal tea for a long time. In this study, two novel (1 and 5) and four known (2, 3, 4 and 6) terpenoids were isolated from the leaves of Rubus corchorifolius L. f. Structural analysis was performed using various spectroscopic methods ((1)H NMR, (13)C NMR and MS) to identify the following six compounds: (16α)-16,17,18-trihydroxy-ent-kauran-18-O-β-d-glucoside (1), ent-16β,17-dialkyl-3-oxygen-kaurane (2), ent-kaurane-3α,16β,17-triol (3), ent-kaurane(5R,8S,9R,10R,13R,16R)-2-one-16α,17-diol (4), (16R)-16β,17,19-trihydroxy-ent-kaur-3-one (5) and ent-16α,17-dihydroxy-kauran-19-oic-acid (6)...
January 30, 2017: Food & Function
https://www.readbyqxmd.com/read/28134352/litchi-seed-extracts-diminish-prostate-cancer-progression-via-induction-of-apoptosis-and-attenuation-of-emt-through-akt-gsk-3%C3%AE-signaling
#17
Hongwei Guo, Hua Luo, Hebao Yuan, Yudui Xia, Pan Shu, Xin Huang, Yi Lu, Xia Liu, Evan T Keller, Duxin Sun, Jiagang Deng, Jian Zhang
Litchi (Litchi chinensisSonnnerat, Sapindaceae), known as Chinese Cherry, is a subtropical fruit tree originating from southern China. Litchi seed extracts have diverse pharmacological effects, including anticancer. However, its anticancer effects and mechanisms on prostate cancer have not been determined. In this study, we used n-butyl alcohol extract of Litchi seed (NLS) to treat prostate cancer PC3, DU145, RM1 and C4-2B cells. NLS induced a significant decrease in cell viability and clonogenic growth in a dose-dependent manner...
January 30, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28131385/comparison-of-three-feline-leukaemia-virus-felv-point-of-care-antigen-test-kits-using-blood-and-saliva
#18
COMPARATIVE STUDY
Mark E Westman, Richard Malik, Evelyn Hall, Paul A Sheehy, Jacqueline M Norris
Feline leukaemia virus (FeLV) can be a challenging infection to diagnose due to a complex feline host-pathogen relationship and occasionally unreliable test results. This study compared the accuracy of three point-of-care (PoC) FeLV p27 antigen test kits commonly used in Australia and available commercially worldwide (SNAP FIV/FeLV Combo, Witness FeLV/FIV and Anigen Rapid FIV/FeLV), using detection of FeLV provirus by an in-house real-time polymerase chain reaction (qPCR) assay as the diagnostic gold standard...
February 2017: Comparative Immunology, Microbiology and Infectious Diseases
https://www.readbyqxmd.com/read/28127702/effect-of-sodium-butyrate-on-cell-proliferation-and-cell-cycle-in-porcine-intestinal-epithelial-ipec-j2-cells
#19
Yueqin Qiu, Xianyong Ma, Xuefen Yang, Li Wang, Zongyong Jiang
Conflicting results have been reported that butyrate in normal piglets leads either to an increase or to a decrease of jejunal villus length, implying a possible effect on the proliferation of enterocytes. No definitive study was found for the biological effects of butyrate in porcine jejunal epithelial cells. The present study used IPEC-J2 cells, a non-transformed jejunal epithelial line to evaluate the direct effects of sodium butyrate on cell proliferation, cell cycle regulation, and apoptosis. Low concentrations (0...
January 27, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28126968/cd244-maintains-the-proliferation-ability-of-leukemia-initiating-cells-through-shp-2-p27kip1-signaling
#20
Feifei Zhang, Xiaoye Liu, Chiqi Chen, Jun Zhu, Zhuo Yu, Jingjing Xie, Li Xie, Haitao Bai, Yaping Zhang, Xia Fang, Hao Gu, Chun Wang, Wei Weng, Cheng Cheng Zhang, Guo-Qiang Chen, Aibing Liang, Junke Zheng
Targeting leukemia initiating cells is considered to be an effective way to cure leukemia, for which it is critical to identify novel therapeutic targets. Herein, we demonstrate that CD244, which is initially reported as a key regulator for NK cells, is highly expressed on both mouse and human leukemia initiating cells. Upon CD244 knockdown, human leukemia cell lines and primary leukemia cells have markedly impaired proliferation abilities both in vitro and in vivo. Interestingly, the repopulation ability of both mouse and human hematopoietic stem cells is not impaired upon CD244 knockdown...
January 25, 2017: Haematologica
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