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Articular cartilage

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https://www.readbyqxmd.com/read/28535500/transient-receptor-potential-vanilloid-5-mediates-ca2-influx-and-inhibits-chondrocyte-autophagy-in-a-rat-osteoarthritis-model
#1
Yingliang Wei, Yanfang Wang, Yutong Wang, Lunhao Bai
BACKGROUND: Autophagy, a self-protective mechanism of chondrocytes, has become a promising target to impede the progress of osteoarthritis (OA). Autophagy is regulated by cytosolic Ca2+ activity and may thus be modified by the Ca2+ permeable transient receptor potential channel vanilloid 5 (TRPV5). Therefore, we investigated the potential role of TRPV5 in mediating Ca2+ influx and in inhibiting chondrocyte autophagy in a rat OA model. METHODS: The rat OA model was assessed by macroscopic and histological analyses...
May 25, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28534942/gene-expression-analysis-of-growth-factor-receptors-in-human-chondrocytes-in-monolayer-and-3d-pellet-cultures
#2
Anika Witt, Achim Salamon, Diana Boy, Doris Hansmann, Andreas Büttner, Andreas Wree, Rainer Bader, Anika Jonitz-Heincke
The main goal of cartilage repair is to create functional tissue by enhancing the in vitro conditions to more physiological in vivo conditions. Chondrogenic growth factors play an important role in influencing cartilage homeostasis. Insulin‑like growth factor (IGF)‑1 and transforming growth factor (TGF)‑β1 affect the expression of collagen type II (Col2) and glycosaminoglycans (GAGs) and, therefore, the targeted use of growth factors could make chondrogenic redifferentiation more efficient. In the present study, human chondrocytes were postmortally isolated from healthy articular cartilage and cultivated as monolayer or 3D pellet cultures either under normoxia or hypoxia and stimulated with IGF‑1 and/or TGF‑β1 to compare the impact of the different growth factors...
May 18, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28534289/exposure-of-articular-chondrocytes-to-wear-particles-induces-phagocytosis-differential-inflammatory-gene-expression-and-reduced-proliferation
#3
Michael D Kurdziel, Meagan Salisbury, Lige Kaplan, Tristan Maerz, Kevin C Baker
The production of wear debris particulate remains a concern due to its association with implant failure through complex biologic interactions. In the setting of unicompartmental knee arthroplasty (UKA), damage and wear of the components may introduce debris particulate into the adjacent, otherwise, healthy compartment. The purpose of this study was to investigate the in vitro effect of polymeric and metallic wear debris particles on cell proliferation, extracellular matrix regulation, and phagocytosis index of normal human articular chondrocytes (nHACs)...
July 2017: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/28533592/articular-chondrocyte-alignment-in-the-rat-after-surgically-induced-osteoarthritis
#4
Hideaki Takahashi, Hiroyuki Tamaki, Noriaki Yamamoto, Hideaki Onishi
[Purpose] Chondrocytes in articular cartilage are aligned as columns from the joint surface. Notably, loss of chondrocyte and abnormalities of differentiation factors give rise to osteoarthritis (OA). However, the relationship between chondrocyte alignment and OA progression remains unclear. This study was performed to investigate temporal alterations in surgically-induced OA rats. [Subjects and Methods] Thirteen-week-old Wistar rats (n=30) underwent destabilized medial meniscus surgery in their right knee and sham surgery in their left knee...
April 2017: Journal of Physical Therapy Science
https://www.readbyqxmd.com/read/28529661/slipped-capital-femoral-epiphysis-management-and-the-arthroscope
#5
F Accadbled, O May, C Thévenin-Lemoine, J Sales de Gauzy
BACKGROUND: In situ pinning of slipped capital femoral epiphysis (SCFE) results in various degrees of deformity of the femoral head-neck junction. Repetitive trauma from cam-type femoroacetabular impingement (FAI) can lead to labral tears and injury to the articular cartilage causing loss of function. Arthroscopic osteoplasty is an alternative to open procedure and to Southwick/Imhäuser-type osteotomies in symptomatic selected cases. SURGICAL TECHNIQUE: The amount of bone to be resected has to be carefully planned pre-operatively...
April 2017: Journal of Children's Orthopaedics
https://www.readbyqxmd.com/read/28529106/engineered-cartilage-regeneration-from-adipose-tissue-derived-mesenchymal-stem-cells-a-morphomolecular-study-on-osteoblast-chondrocyte-and-apoptosis-evaluation
#6
Marta Anna Szychlinska, Paola Castrogiovanni, Houda Nsir, Michelino Di Rosa, Claudia Guglielmino, Rosalba Parenti, Giovanna Calabrese, Elisabetta Pricoco, Lucia Salvatorelli, Gaetano Magro, Rosa Imbesi, Ali Mobasheri, Giuseppe Musumeci
The poor self-repair capacity of cartilage tissue in degenerative conditions, such as osteoarthritis (OA), has prompted the development of a variety of therapeutic approaches, such as cellular therapies and tissue engineering based on the use of mesenchymal stem cells (MSCs). The aim of this study is to demonstrate, for the first time, that the chondrocytes differentiated from rat adipose tissue derived-MSCs (AMSCs), are able to constitute a morphologically and biochemically healthy hyaline cartilage after 6 weeks of culture on a Collagen Cell Carrier (CCC) scaffold...
May 18, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28528301/selective-laser-sintering-scaffold-with-hierarchical-architecture-and-gradient-composition-for-osteochondral-repair-in-rabbits
#7
Yingying Du, Haoming Liu, Qin Yang, Shuai Wang, Jianglin Wang, Jun Ma, Insup Noh, Antonios G Mikos, Shengmin Zhang
Osteochondral defects cannot be adequately self-repaired due to the presence of the sophisticated hierarchical structure and the lack of blood supply in cartilage. Thus, one of the major challenges remaining in this field is the structural design of a biomimetic scaffold that satisfies the specific requirements for osteochondral repair. To address this hurdle, a bio-inspired multilayer osteochondral scaffold that consisted of the poly(ε-caprolactone) (PCL) and the hydroxyapatite (HA)/PCL microspheres, was constructed via selective laser sintering (SLS) technique...
May 12, 2017: Biomaterials
https://www.readbyqxmd.com/read/28526923/focal-articular-prosthetic-resurfacing-for-the-treatment-of-full-thickness-articular-cartilage-defects-in-the-knee-12-year-follow-up-of-two-cases-and-review-of-the-literature
#8
C Becher, E B Cantiller
INTRODUCTION: The rationale of focal articular prosthetic resurfacing used as a primary arthroplasty procedure in the treatment of articular cartilage defects is still under debate. Conflicting reports raise concern about high rates of re-operations and continued development of osteoarthritis, while others have reported good outcomes. The goal of this paper is to present the long-term results of two patients with a 12-year follow-up and to report the results of a literature review. MATERIALS AND METHODS: Two patients (male, 70 years; female 63 years) with a follow-up of 12 years were reviewed...
May 19, 2017: Archives of Orthopaedic and Trauma Surgery
https://www.readbyqxmd.com/read/28521989/synthesis-of-strontium-chondroitin-sulfate-and-the-evaluation-of-its-capability-to-attenuate-osteoarthritis
#9
F B Ma, N Liu, N Hu, C Y Wen, B Tang
Osteoarthritis (OA) is the most prevalent musculoskeletal disorder and the leading cause of joint disability in elderly patients. In this study, we fabricated strontium chondroitin sulfate (SrCS), a new polysaccharide-metal ion complex that is the combination of chondroitin sulfate and strontium, which are two widely adopted chemicals in OA clinical management. The structural, chemical compositions and morphology of as-fabricated SrCS were systematically investigated. Cell proliferation test, RT-PCR and preliminary animal studies were conducted to evaluate the clinical potential of SrCS on OA treatment...
August 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28521277/the-spatio-temporal-mechanical-environment-of-healthy-and-injured-human-cartilage-during-sustained-activity-and-its-role-in-cartilage-damage
#10
Saeed Miramini, David W Smith, Lihai Zhang, Bruce S Gardiner
Recently we presented a computational model of articular cartilage calibrated for normal human tissue explants. This model was able to capture the transient deformation of cartilage experiencing a cyclic load. The model takes into account the tension-compression nonlinearity of the cartilage and incorporates the dependency of the compressive stiffness and fluid permeability of cartilage on the deformation-dependent aggrecan concentration in cartilage tissue. As such it represents a leading constitutive model of articular cartilage...
May 10, 2017: Journal of the Mechanical Behavior of Biomedical Materials
https://www.readbyqxmd.com/read/28520463/a-preclinical-assessment-of-early-continuous-passive-motion-and-treadmill-therapeutic-exercises-for-generating-chondroprotective-effects-after-anterior-cruciate-ligament-rupture
#11
Nai-Jen Chang, Kuan-Wei Lee, Chih-Jou Chu, Ming-You Shie, Pei-Hsi Chou, Chih-Chan Lin, Peir-In Liang
BACKGROUND: Anterior cruciate ligament (ACL) injury is a well-known risk factor for the development of posttraumatic osteoarthritis (PTOA). However, whether using continuous passive motion (CPM) with or without additional treadmill exercise (TRE) in early ACL injury might provide chondroprotective effects and further decrease the risk of PTOA has yet to be determined. HYPOTHESIS: CPM may offer an enhanced chondroprotective effect, but TRE may attenuate that effect due to the mechanical stress on the joint and inflammatory cytokines in the joint...
May 1, 2017: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/28520086/activated-fgfr3-prevents-subchondral-bone-sclerosis-during-the-development-of-osteoarthritis-in-transgenic-mice-with-achondroplasia
#12
Toshiaki Okura, Masaki Matsushita, Kenichi Mishima, Ryusaku Esaki, Taisuke Seki, Naoki Ishiguro, Hiroshi Kitoh
The purpose of this study is to investigate the morphometric changes of the subchondral bone during the development of osteoarthritis (OA) in transgenic mice with achondroplasia (Fgfr3(ach) ) carrying a heterozygous gain-of-function mutation in Fgfr3. Two OA models (spontaneously developed with age: the aging model, and surgically induced by destabilization of the medial meniscus: the DMM model) were established. Articular cartilage, epiphysis and metaphysis of the knee joint were histologically and morphometrically compared between wild-type mice and Fgfr3(ach) mice in both OA models...
May 18, 2017: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://www.readbyqxmd.com/read/28518064/an-experimental-and-finite-element-protocol-to-investigate-the-transport-of-neutral-and-charged-solutes-across-articular-cartilage
#13
Vahid Arbabi, Behdad Pouran, Amir A Zadpoor, Harrie Weinans
Osteoarthritis (OA) is a debilitating disease that is associated with degeneration of articular cartilage and subchondral bone. Degeneration of articular cartilage impairs its load-bearing function substantially as it experiences tremendous chemical degradation, i.e. proteoglycan loss and collagen fibril disruption. One promising way to investigate chemical damage mechanisms during OA is to expose the cartilage specimens to an external solute and monitor the diffusion of the molecules. The degree of cartilage damage (i...
April 23, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28516738/intrinsic-fluorescence-and-mechanical-testing-of-articular-cartilage-in-human-patients-with-osteoarthritis
#14
Juan Pablo Padilla-Martinez, William Lewis, Antonio Ortega-Martinez, Walfre Franco
The degeneration of articular cartilage is the main cause of osteoarthritis (OA), a common cause of disability among elderly patients. The aim of this study is to understand the correlation between intrinsic fluorescence of articular cartilage and its biomechanical properties in patients with osteoarthritis. Cylindrical samples of articular cartilage 6 mm in diameter were extracted via biopsy punch from the femoral condyles of 6 patients with advanced OA undergoing knee replacement surgery. The mechanical stiffness and fluorescence of each cartilage plug were measured by indentation test and spectrofluorometry...
May 18, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28516048/image-interpolation-improves-the-zonal-analysis-of-cartilage-t2-relaxation-in-mri
#15
Farid Badar, Yang Xia
BACKGROUND: This project aimed to investigate the improvement in the detection of osteoarthritis (OA) in cartilage by the interpolation of T2 images, in the situation when the native MRI resolution is insufficient to resolve the depth-dependent T2 characteristics in articular cartilage (AC). METHODS: Eighteen intact canine knee joints that were healthy or had mild (contralateral) or severe OA were T2-imaged in a 7T/20 cm MRI system at 200 µm/pixel resolution (macro-MRI)...
April 2017: Quantitative Imaging in Medicine and Surgery
https://www.readbyqxmd.com/read/28515058/tissue-engineered-cartilage-constructed-by-a-biotin-conjugated-anti-cd44-avidin-binding-technique-for-the-repairing-of-cartilage-defects-in-the-weight-bearing-area-of-knee-joints-in-pigs
#16
H Lin, J Zhou, L Cao, H R Wang, J Dong, Z R Chen
OBJECTIVES: The lack of effective treatment for cartilage defects has prompted investigations using tissue engineering techniques for their regeneration and repair. The success of tissue-engineered repair of cartilage may depend on the rapid and efficient adhesion of transplanted cells to a scaffold. Our aim in this study was to repair full-thickness defects in articular cartilage in the weight-bearing area of a porcine model, and to investigate whether the CD44 monoclonal antibody biotin-avidin (CBA) binding technique could provide satisfactory tissue-engineered cartilage...
May 2017: Bone & Joint Research
https://www.readbyqxmd.com/read/28513981/exposure-to-fermentation-supernatant-of-staphylococcus-aureus-accelerated-dedifferentiation-of-chondrocytes-and-production-of-antimicrobial-peptides
#17
Shuaijun Li, Yun Zhang, Yidan Sun, Weigang Cao, Lei Cui
Staphylococcus aureus is the most popular pathogen found in septic arthritis. Despite bacteria was eradicated from joint cavity during acute infection, destruction of articular cartilage often continues for years, leading to permanent joint damage. The mechanism responsible for this consistent catabolic reaction in septic arthritis remains unclear. Here, we found that fermentation supernatant (FS) of Staphylococcus aureus accelerated dedifferentiation of chondrocytes and induced expression of catabolic factors including A Disintegrin-like and Metalloproteinase with Thrombospondin-1 motifs 5, NO synthase 2, matrix metalloproteinase -3, -13...
May 17, 2017: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://www.readbyqxmd.com/read/28513840/histomorphology-and-innate-immunity-during-the-progression-of-osteoarthritis-does-synovitis-affect-cartilage-degradation
#18
Huan Wang, Qingguo Wang, Meijuan Yang, Lili Yang, Weili Wang, Haobin Ding, Dong Zhang, Jing Xu, Xuezhang Tang, Haitao Ding, Qingfu Wang
Osteoarthritis (OA) is a common chronic degenerative disease that affects all joints. At present, the pathological processes and mechanisms of OA are still unclear. Innate immunity, a key player in damage to the structure of the joint and the mechanism by which the host attempts to repair OA, affects all pathological stages of the disease. In the present study, our aim was to assess changes in innate immunity during the pathological processes of OA in articular cartilage (AC) and the synovial membrane (SM), which are the major structures in joints, and to systematically examine the histological changes in AC and SM in mild, moderate and severe cases of OA, in order to further speculate about the manner in which the interactions of AC and SM are facilitated by innate immunity...
May 17, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28512850/in-situ-handheld-3d-bioprinting-for-cartilage-regeneration
#19
Claudia Di Bella, Serena Duchi, Cathal D O'Connell, Romane Blanchard, Cheryl Augustine, Zhilian Yue, Fletcher Thompson, Christopher Richards, Stephen Beirne, Carmine Onofrillo, Sebastien H Bauquier, Stewart D Ryan, Peter Pivonka, Gordon G Wallace, Peter F Choong
Articular cartilage injuries experienced at an early age can lead to the development of osteoarthritis later in life. In situ 3D printing is an exciting and innovative bio-fabrication technology that enables the surgeon to deliver tissue- engineering techniques at the time and location of need. We have created a hand- held 3D printing device (Biopen) that allows the simultaneous co-axial extrusion of bioscaffold and cultured cells directly into the cartilage defect in vivo in a single session surgery. This pilot study assesses the ability of the Biopen to repair a full thickness chondral defect and the early outcomes in cartilage regeneration, and compares these results to other treatments in a large animal model...
May 17, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28512729/protective-effects-of-nebivolol-against-interleukin-1%C3%AE-il-1%C3%AE-induced-type-ii-collagen-destruction-mediated-by-matrix-metalloproteinase-13-mmp-13
#20
Zhigang Li, Baoyi Liu, Dewei Zhao, BenJie Wang, Yupeng Liu, Yao Zhang, Fengde Tian, Borui Li
The pathological progression of osteoarthritis (OA) involves degradation of articular cartilage matrix. Type II collagen is the main component of cartilage matrix, which is degraded by pro-inflammatory cytokines such as IL-1β mediated by MMP-13. Nebivolol, a licensed drug used for the treatment of hypertension in clinics, displays its anti-inflammatory capacity in various conditions. However, whether Nebivolol has a protective effect on cartilage matrix degradation has not been reported before. In this study, we investigated the effects of Nebivolol on regulating the expression of MMP-13 and degradation of type II collagen...
May 17, 2017: Cell Stress & Chaperones
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