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https://www.readbyqxmd.com/read/29673954/oxidative-stress-in-osteoarthritis-and-antioxidant-effect-of-polysaccharide-from-angelica-sinensis
#1
Chao Zhuang, Yuji Wang, Yunkun Zhang, Nanwei Xu
This study aimed to investigate the oxidative stress in human osteoarthritis (OA) chondrocytes and the antioxidant effect of angelica sinensis polysaccharide (ASP) on it. Human OA chondrocytes and human normal chondrocytes induced by hydrogen peroxide (H2 O2 ) alone or treated with ASP were cultured, then they were measured with cell viability, levels of reactive oxygen species (ROS), nitric oxide (NO) and malondialdehyde (MDA) production, activities of inducible nitric oxide synthase (iNOS), superoxide dismutase (SOD) and catalase (CAT), expression of related genes, such as collagen type II (Col2a1), Aggrecan, iNOS, SOD, CAT and peroxisome proliferator-activated receptor gamma (PPARγ)...
April 16, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29673343/anti-osteoarthritic-effects-of-chondrot-in-a-rat-model-of-collagenase-induced-osteoarthritis
#2
Jiwon Jeong, Kiljoon Bae, Sun-Gil Kim, Dongwook Kwak, Young-Joo Moon, Chan-Hun Choi, Young-Ran Kim, Chang-Su Na, Seon-Jong Kim
BACKGROUND: Previously, we reported that ChondorT showed significant anti-arthritis and anti-inflammatory effects. ChondroT, a new herbal medication, consists of the water extracts of Osterici Radix, Lonicerae Folium, Angelicae Gigantis Radix, Clematidis Radix, and Phellodendri Cortex (6:4:4:4:3). The objective of this study was to investigate the effects of ChondroT in collagenase-induced osteoarthritis rat model. METHODS: Osteoarthritis was induced by the injection of collagenase into the right knee joint cavity of rats...
April 19, 2018: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/29672434/assessment-of-cartilage-growth-after-biopsy-of-osteochondral-loose-bodies-in-adolescent-knees-for-use-in-autologous-chondrocyte-implantation
#3
Sean Robinson, Jonathan Kramer, Trevor Shelton, Jarrad Merriman, Brian Haus
BACKGROUND: The goal of this study is to determine whether harvested cartilage from an osteochondral loose body maintains the same viability for implantation as cartilage harvested from the traditional locations within the adolescent knee for autologous chondrocyte implantation (ACI). METHODS: A retrospective study was performed on all ACI procedures performed from 2014 to 2017 at a single institution. Biopsies were derived from 2 groups: osteochondral loose body verses the intercondylar notch...
April 18, 2018: Journal of Pediatric Orthopedics
https://www.readbyqxmd.com/read/29671342/human-articular-chondrocytes-retain-their-phenotype-in-sustained-hypoxia-while-normoxia-promotes-their-immunomodulatory-potential
#4
Claire Mennan, John Garcia, Helen McCarthy, Sharon Owen, Jade Perry, Karina Wright, Robin Banerjee, James B Richardson, Sally Roberts
Objective To assess the phenotype of human articular chondrocytes cultured in normoxia (21% O2 ) or continuous hypoxia (2% O2 ). Design Chondrocytes were extracted from patients undergoing total knee replacement ( n = 5) and cultured in ~21% (normoxic chondrocytes, NC) and 2% (hypoxic chondrocytes, HC) oxygen in both monolayer and 3-dimensional (3D) pellet culture and compared with freshly isolated chondrocytes (FC). Cells were assessed by flow cytometry for markers indicative of mesenchymal stromal cells (MSCs), chondrogenic-potency and dedifferentiation...
April 1, 2018: Cartilage
https://www.readbyqxmd.com/read/29670657/effects-of-qi-fang-xi-bi-granules-on-cartilage-morphology-and-c-ebp-%C3%AE-promoter-methylation-in-rats-with-knee-osteoarthritis
#5
Xinxin Wang, Peng Shen, Dezhi Tang, Hao Xu, Hongfu Qiu, Tao Wu, Xiang Gao
Objective: To investigate the effects of Qi-Fang-Xi-Bi-Granules (QFXBGs) on cartilage morphology and methylation of C/ebp α (CCAAT/enhancer binding protein α ) at the promoter region. Methods: Knee osteoarthritis (KOA) modeling was performed in rats in accordance with Hulth's method, and control group received sham operation. Eight weeks after KOA modeling, the rats in the KOA modeling group were further divided into 6 groups. Each group was given the appropriate drug...
2018: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/29668079/co-culture-of-adipose-derived-stem-cells-and-chondrocytes-on-three-dimensionally-printed-bioscaffolds-for-craniofacial-cartilage-engineering
#6
Robert J Morrison, Hassan B Nasser, Khaled N Kashlan, David A Zopf, Derek J Milner, Colleen L Flanangan, Matthew B Wheeler, Glenn E Green, Scott J Hollister
OBJECTIVES/HYPOTHESIS: Reconstruction of craniofacial cartilagenous defects are among the most challenging surgical procedures in facial plastic surgery. Bioengineered craniofacial cartilage holds immense potential to surpass current reconstructive options, but limitations to clinical translation exist. We endeavored to determine the viability of utilizing adipose-derived stem cell-chondrocyte co-culture and three-dimensional (3D) printing to produce 3D bioscaffolds for cartilage tissue engineering...
April 18, 2018: Laryngoscope
https://www.readbyqxmd.com/read/29667446/intra-articular-injection-of-kartogenin-conjugated-polyurethane-nanoparticles-attenuates-the-progression-of-osteoarthritis
#7
Wenshuai Fan, Jinghuan Li, Liu Yuan, Jifei Chen, Zhe Wang, Yiming Wang, Changan Guo, Xiumei Mo, Zuoqin Yan
Osteoarthritis (OA) is the most common form of joint disease and a leading cause of physical disability, there is an urgent need to attenuate the progression of OA. Intra-articular (IA) injection is an effective treatment for joints diseases, however, the therapeutic effects mostly depend on the efficacy of drug duration in joints. Drug delivery system can provide drug-controlled release and reduce the number of IA injection. In this study, amphiphilic polyurethanes with pendant amino group were synthesized and amide bonds were formed between the amine group of polyurethane and the carboxyl group of kartogenin (KGN), a small molecular reported to show both regenerative and protective effects on cartilage...
November 2018: Drug Delivery
https://www.readbyqxmd.com/read/29666896/calpain-inhibition-attenuates-bleomycin-induced-pulmonary-fibrosis-via-switching-the-development-of-epithelial-mesenchymal-transition
#8
Yuan Liu, Bing Liu, Gu-Qin Zhang, Jing-Feng Zou, Meng-Lin Zou, Zhen-Shun Cheng
Calpains are intracellular calcium-dependent cysteine proteases, which cleave several substrates proteins, have been proven to play important roles in lung fibrosis. The aim of this study was to investigate the effects of calpain on bleomycin (BLM)-induced pulmonary fibrosis. A lung fibrosis mice model was established successfully by intraperitoneal injection of bleomycin. Calpeptin, a highly selective inhibitor of calpain activation, was administered three times weekly after bleomycin injection. Histological examination was used to assess the fibrosis...
April 18, 2018: Naunyn-Schmiedeberg's Archives of Pharmacology
https://www.readbyqxmd.com/read/29666250/developmentally-inspired-programming-of-adult-human-mesenchymal-stromal-cells-toward-stable-chondrogenesis
#9
Paola Occhetta, Sebastien Pigeot, Marco Rasponi, Boris Dasen, Arne Mehrkens, Thomas Ullrich, Ina Kramer, Sabine Guth-Gundel, Andrea Barbero, Ivan Martin
It is generally accepted that adult human bone marrow-derived mesenchymal stromal cells (hMSCs) are default committed toward osteogenesis. Even when induced to chondrogenesis, hMSCs typically form hypertrophic cartilage that undergoes endochondral ossification. Because embryonic mesenchyme is obviously competent to generate phenotypically stable cartilage, it is questioned whether there is a correspondence between mesenchymal progenitor compartments during development and in adulthood. Here we tested whether forcing specific early events of articular cartilage development can program hMSC fate toward stable chondrogenesis...
April 16, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29664223/chondrogenic-response-in-presence-of-cartilage-extracellular-matrix-nanoparticles
#10
Saeed Zahiri, Elahe Masaeli, Elahe Poorazizi, Mohammad Hossein Nasr-Esfahani
Current studies based on regenerative medicine suggest, decellularized extracellular matrix (DC-ECM) components can regulate cell phenotype. In this regard, it is believed, presence of cartilage extracellular matrix particles in culture condition could produce physical and biochemical supportive cues for chondrogenesis. In this study, DC-ECM nanoparticles with average size of 61.5± 22.4 nm were produced by decellularization and mechanical processing. Homogenous distribution and nano scale size of yield particles were observed by Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM) microscopy imaging...
April 17, 2018: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/29663644/biomimetic-sulfated-polyethylene-glycol-hydrogel-inhibits-proteoglycan-loss-and-tumor-necrosis-factor-%C3%AE-induced-expression-pattern-in-an-osteoarthritis-in-vitro-model
#11
Shabnam Hemmati-Sadeghi, Pradip Dey, Jochen Ringe, Rainer Haag, Michael Sittinger, Tilo Dehne
This study aimed to evaluate the potential of an anti-inflammatory polyethylene glycol (PEG) hydrogel for osteoarthritis (OA) management in an OA in vitro model. Freshly isolated porcine chondrocytes were maintained in high-density cultures to form cartilage-like three-dimensional micromasses. Recombinant porcine tumor necrosis factor-alpha (TNF-α) was used to induce OA-like changes. Normal and OA-like micromasses were treated with dendritic polyglycerol sulfate-based PEG hydrogel. Live/dead staining showed that all micromasses remained vital and presented similar morphological characteristics...
April 16, 2018: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/29663508/expression-of-endoplasmic-reticulum-stress-protein-in-rabbit-condyle-cartilage-following-anterior-disc-displacement
#12
Ting Xu, Zhiyuan Gu, Huiling Wu, Hua Yao, Guohua Wang
OBJECTIVE: Anterior disc displacement (ADD) is a most common subtype of temporomandibular disorders (TMD), which is promoted by chondrocytes apoptosis. However, the signaling pathways that trigger apoptosis are still unknown. The aim of this study was to investigate the expression of endoplasmic reticulum (ER) stress related proteins in the condylar cartilage of rabbits following ADD. METHODS: Sixty healthy adult rabbits were randomly assigned to the experimental and sham-operated control groups (n=12)...
April 16, 2018: Journal of Oral Pathology & Medicine
https://www.readbyqxmd.com/read/29662780/distinct-metabolic-programs-induced-by-tgf-%C3%AE-1-and-bmp2-in-human-articular-chondrocytes-with-osteoarthritis
#13
Cuicui Wang, Richard M Silverman, Jie Shen, Regis J O'Keefe
Objectives: Cellular energy metabolism is important for the function of all tissues, including cartilage. Recent studies indicate that superficial and deep subpopulations of articular chondrocytes (ACs) have distinct metabolic profiles. At the cellular and molecular level, osteoarthritis (OA) is characterised by alteration from a healthy homoeostatic state towards a catabolic state. Several molecular pathways, including transforming growth factor beta (TGF-β) and bone morphogenetic protein (BMP) signalling, have been identified as critical players in the pathogenesis and progression of OA...
January 2018: Journal of Orthopaedic Translation
https://www.readbyqxmd.com/read/29662535/adipose-tissue-stem-cells-in-regenerative-medicine
#14
REVIEW
Vanesa Verónica Miana, Elio A Prieto González
Adipose tissue-derived stem cells (ADSCs) are mesenchymal cells with the capacity for self-renewal and multipotential differentiation. This multipotentiality allows them to become adipocytes, chondrocytes, myocytes, osteoblasts and neurocytes among other cell lineages. Stem cells and, in particular, adipose tissue-derived cells, play a key role in reconstructive or tissue engineering medicine as they have already proven effective in developing new treatments. The purpose of this work is to review the applications of ADSCs in various areas of regenerative medicine, as well as some of the risks associated with treatment with ADSCs in neoplastic disease...
2018: Ecancermedicalscience
https://www.readbyqxmd.com/read/29660438/prenatal-nicotine-exposure-intergenerationally-programs-imperfect-articular-cartilage-via-histone-deacetylation-through-maternal-lineage
#15
Zhe Xie, Zhe Zhao, Xu Yang, Linguo Pei, Hanwen Luo, Qubo Ni, Bin Li, Yongjian Qi, Kai Tie, Jacques Magdalou, Liaobin Chen, Hui Wang
Accumulating evidence has shown that the impact of prenatal environmental factors on the organs of the offspring could last until the adulthood. Here, we aimed to investigate these effects and the potential mechanism of prenatal nicotine exposure (PNE) on the female adult cartilage of the first generation (PNE-F1) and the second generation (PNE-F2). Pregnant Wistar rats were injected with 2.0 mg/kg.d nicotine from gestational day (GD) 9 to 20. Then their F1 generation at GD20 and postnatal week (PW) 12, and F2 generation at PW12 were harvested...
April 13, 2018: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/29659837/noonan-syndrome-causing-shp2-mutants-impair-erk-dependent-chondrocyte-differentiation-during-endochondral-bone-growth
#16
Mylène Tajan, Julie Pernin-Grandjean, Nicolas Beton, Isabelle Gennero, Florence Capilla, Benjamin G Neel, Toshiyuki Araki, Philippe Valet, Maithé Tauber, Jean-Pierre Salles, Armelle Yart, Thomas Edouard
Growth retardation is a constant feature of Noonan syndrome (NS) but its physiopathology remains poorly understood. We previously reported that hyperactive NS-causing SHP2 mutants impair the systemic production of insulin-like growth factor 1 (IGF1) through hyperactivation of the RAS/extracellular signal-regulated kinases (ERK) signalling pathway. Besides endocrine defects, a direct effect of these mutants on growth plate has not been explored, although recent studies have revealed an important physiological role for SHP2 in endochondral bone growth...
April 12, 2018: Human Molecular Genetics
https://www.readbyqxmd.com/read/29659575/synergistic-co-regulation-and-competition-by-a-sox9-gli-foxa-phasic-transcriptional-network-coordinate-chondrocyte-differentiation-transitions
#17
Zhijia Tan, Ben Niu, Kwok Yeung Tsang, Ian G Melhado, Shinsuke Ohba, Xinjun He, Yongheng Huang, Cheng Wang, Andrew P McMahon, Ralf Jauch, Danny Chan, Michael Q Zhang, Kathryn S E Cheah
The growth plate mediates bone growth where SOX9 and GLI factors control chondrocyte proliferation, differentiation and entry into hypertrophy. FOXA factors regulate hypertrophic chondrocyte maturation. How these factors integrate into a Gene Regulatory Network (GRN) controlling these differentiation transitions is incompletely understood. We adopted a genome-wide whole tissue approach to establish a Growth Plate Differential Gene Expression Library (GP-DGEL) for fractionated proliferating, pre-hypertrophic, early and late hypertrophic chondrocytes, as an overarching resource for discovery of pathways and disease candidates...
April 16, 2018: PLoS Genetics
https://www.readbyqxmd.com/read/29659379/bony-reconstruction-of-the-anterior-glenoid-rim
#18
Laurent B Willemot, Bassem T Elhassan, Olivier Verborgt
Recurrent anterior shoulder instability is associated with glenohumeral bone loss. Glenoid deficiency compromises the concavity-compression mechanism. Medial Hill-Sachs lesions can result in an off-track humeral position. Anterior glenoid reconstruction or augmentation prevents recurrence by addressing the pathomechanics. In Bristow and Latarjet procedures, the coracoid process is harvested for conjoint tendon transfer, capsular reinforcement, and glenoid rim restoration. Complications and the nonanatomic nature of the procedure have spurred research on graft sources...
April 13, 2018: Journal of the American Academy of Orthopaedic Surgeons
https://www.readbyqxmd.com/read/29659132/3d-extrusion-printing-induces-polymer-molecule-alignment-and-cell-organization-within-engineered-cartilage
#19
Ting Guo, Julia P Ringel, Casey G Lim, Laura G Bracaglia, Maeesha Noshin, Hannah B Baker, Douglas A Powell, John P Fisher
Proper cell-material interactions are critical to remain cell function and thus successful tissue regeneration. Many fabrication processes have been developed to create microenvironments to control cell attachment and organization on a 3D scaffold. However, these approaches often involve heavy engineering and only the surface layer can be patterned. We found that 3D extrusion based printing at high temperature and pressure will result an aligned effect on the polymer molecules, and this molecular arrangement will further induce the cell alignment and different differentiation capacities...
April 16, 2018: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/29658381/hydrogen-peroxide-a-potent-inducer-of-differentiation-of-human-adipose-derived-stem-cells-into-chondrocytes
#20
Farjam Goudarzi, Adel Mohammadalipour, Majid Bahabadi, Mohammad Taghi, Goodarzi, Arash Sarveazad, Iraj Khodadadi
Common protocols for chondrogenic differentiation of adipose-derived mesenchymal stem cells (ADSCs) are generally expensive and time-consuming and so far, have not successfully recreated pure chondrocytes. We hypothesize that a low level of H2 O2 may induce differentiation of ADSCs into chondrocytes in a shorter incubation time and relatively lower cost. Therefore, this study aimed to comparatively investigate the effectiveness of H2 O2 -containing or free medium in the induction of ADSCs to chondrocytes. ADSCs were isolated from the lipoaspirate of four healthy females and evaluated by immunophenotyping for their CD90, CD73, CD44, CD34, and CD45 cell surface markers...
April 15, 2018: Free Radical Research
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