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https://www.readbyqxmd.com/read/28823024/effect-of-kaempferia-parviflora-extract-on-knee-osteoarthritis
#1
Hiroko Kobayashi, Ryo Suzuki, Kei Sato, Takatoshi Ogami, Hiroshi Tomozawa, Masahito Tsubata, Koji Ichinose, Masaki Aburada, Wataru Ochiai, Kiyoshi Sugiyama, Tsutomu Shimada
Knee osteoarthritis (OA) is becoming more prevalent worldwide due to increases in the numbers of elderly and obese patients. Currently, pharmaceutical medicines used for the treatment of OA are for symptomatic therapy and therefore new therapeutic agents are needed. Kaempferia parviflora (KP) is a plant growing naturally in Southeast Asia and has various pharmacological effects including an anti-inflammatory effect, but no effect on OA has yet been reported. We therefore conducted a search for the effects KP and the active components of KP extract (KPE) exert on OA as well as its mechanism of action...
August 19, 2017: Journal of Natural Medicines
https://www.readbyqxmd.com/read/28821957/co-culture-of-chondrons-and-mesenchymal-stromal-cells-reduces-the-loss-of-collagen-vi-and-improves-extracellular-matrix-production
#2
H A Owida, T De Las Heras Ruiz, A Dhillon, Y Yang, N J Kuiper
Adult articular chondrocytes are surrounded by a pericellular matrix (PCM) to form a chondron. The PCM is rich in hyaluronan, proteoglycans, and collagen II, and it is the exclusive location of collagen VI in articular cartilage. Collagen VI anchors the chondrocyte to the PCM. It has been suggested that co-culture of chondrons with mesenchymal stromal cells (MSCs) might enhance extracellular matrix (ECM) production. This co-culture study investigates whether MSCs help to preserve the PCM and increase ECM production...
August 19, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28821460/long-term-effects-of-hydrogen-sulfide-on-the-anabolic-catabolic-balance-of-articular-cartilage-in-vitro
#3
A Vela-Anero, T Hermida-Gomez, L Gato-Calvo, C Vaamonde-García, S Diaz-Prado, R Meijide-Faílde, F J Blanco, E F Burguera
Healthy cartilage maintenance relies on an equilibrium among the anabolic and catabolic processes in chondrocytes. With the onset of osteoarthritis (OA), increased interleukin (IL)-1β levels induce an inhibition of the synthesis of extracellular matrix (ECM) proteins, as well as an increase in proteases. This eventually leads to a predominance of the catabolic phenotype and the progressive loss of articular cartilage. Hydrogen sulfide (H2S) is a small gaseous molecule recognized as the third endogenous gasotransmitter...
August 15, 2017: Nitric Oxide: Biology and Chemistry
https://www.readbyqxmd.com/read/28819366/paracrine-potential-of-the-human-adipose-tissue-derived-stem-cells-to-modulate-balance-between-matrix-metalloproteinases-and-their-inhibitors-in-the-osteoarthritic-cartilage-in-vitro
#4
Jaroslav Denkovskij, Edvardas Bagdonas, Ilona Kusleviciute, Zygmunt Mackiewicz, Ausra Unguryte, Narunas Porvaneckas, Sandrine Fleury, Algirdas Venalis, Christian Jorgensen, Eiva Bernotiene
Adipose tissue represents an abundant source of stem cells. Along with anti-inflammatory effects, ASC secrete various factors that may modulate metabolism of extracellular matrix in osteoarthritic (OA) cartilage, suggesting that the presence of ASC could be advantageous for OA cartilage due to the recovery of homeostasis between matrix metalloproteinases (MMPs) and their tissue inhibitors of metalloproteinases (TIMPs). To evaluate these effects, cartilage explants (CE) were cocultured with ASC for 3 and 7 days under stimulation with or without IL-1β...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28818159/effects-of-iron-administration-on-the-diameter-of-cells-of-growth-cartilage-of-rat-pups-during-pregnancy
#5
Faiza Umbreen, Khadija Qamar, Sadia Shaukat, Amna Tasawar
OBJECTIVE: To determine the effect of oral iron administration on pregnant rats on the diameter of cells of growth plate of rat pups. STUDY DESIGN: Experimental study. PLACE AND DURATION OF STUDY: Anatomy Department, Army Medical College, Rawalpindi in collaboration with National Institute of Health (NIH), Islamabad from March to November 2016. METHODOLOGY: Group Acontaining 8 pregnant rats was control group, and group B containing same number of pregnant rats was the study group...
July 2017: Journal of the College of Physicians and Surgeons—Pakistan: JCPSP
https://www.readbyqxmd.com/read/28817810/tgf-%C3%AE-1-is-involved-in-vitamin-d-induced-chondrogenic-differentiation-of-bone-marrow-derived-mesenchymal-stem-cells-by-regulating-the-erk-jnk-pathway
#6
Xiaorui Jiang, Botao Huang, Huiying Yang, Guishi Li, Chunlei Zhang, Guangshi Yang, Feng Lin, Guodong Lin
BACKGROUND/AIMS: Osteoarthritis (OA) is characterized by degradation of cartilage, sole cell type of which is chondrocytes. Bone marrow-derived mesenchymal stem cells (BMSCs) possess multipotency and can be directionally differentiated into chondrocytes under stimulation. This study was aimed to explore the possible roles of vitamin D and transforming growth factor-β1 (TGF-β1) in the chondrogenic differentiation of BMSCs. METHODS: BMSCs were isolated from femurs and tibias of rats and characterized by flow cytometry...
August 16, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28816826/tissue-engineered-bone-differentiated-from-human-adipose-derived-stem-cells-inhibit-posterolateral-fusion-in-an-athymic-rat-model
#7
Comron Saifi, Jonahtan Bernhard, Jamal N Shillingford, Petros Petridis, Samuel Robinson, X Edward Guo, Mark Weidenbaum, Ronald A Lehman, Howard S An, Lawrence G Lenke, Gordana Vunjak-Novakovic, Joseph L Laratta
STUDY DESIGN: Biological augmentation spinal arthrodesis trial in athymic rats OBJECTIVE.: To assess the efficacy of tissue-engineered bone to promote L4-L5 intertransverse process fusion in an athymic rat model. SUMMARY OF BACKGROUND DATA: Each year in the United States, over 400,000 spinal fusion surgeries are performed requiring bone graft. The current gold standard for posterolateral lumbar fusion is autogenous iliac crest bone graft (ICBG), but the harvesting of ICBG is associated with increased operative time and significant complications...
August 14, 2017: Spine
https://www.readbyqxmd.com/read/28815373/autologous-nasal-chondrocytes-delivered-by-injectable-hydrogel-for-in-vivo-articular-cartilage-regeneration
#8
Wenliang Chen, Changhua Li, Maoxiu Peng, Bingju Xie, Lei Zhang, Xiaojun Tang
Cell based tissue engineering serves as a promising strategy for articular cartilage repair, which remains a challenge both for researchers and clinicians. The aim of this research was to assess the potential of autologous nasal chondrocytes (NCs) combined with alginate hydrogel as injectable constructs for rabbit articular cartilage repair. Autologous nasal chondrocytes were isolated from rabbit nasal septum, expanded either on monolayer or in 3D alginate hydrogel. In vitro, DNA quantification revealed that NCs can proliferate stable in 3D alginate matrix, but slower than that cultured in monolayer...
August 16, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28813497/high-hydrostatic-pressure-induces-pro-osteoarthritic-changes-in-cartilage-precursor-cells-a-transcriptome-analysis
#9
Kevin Montagne, Yasuko Onuma, Yuzuru Ito, Yasuhiko Aiki, Katsuko S Furukawa, Takashi Ushida
Due to the high water content of cartilage, hydrostatic pressure is likely one of the main physical stimuli sensed by chondrocytes. Whereas, in the physiological range (0 to around 10 MPa), hydrostatic pressure exerts mostly pro-chondrogenic effects in chondrocyte models, excessive pressures have been reported to induce detrimental effects on cartilage, such as increased apoptosis and inflammation, and decreased cartilage marker expression. Though some genes modulated by high pressure have been identified, the effects of high pressure on the global gene expression pattern have still not been investigated...
2017: PloS One
https://www.readbyqxmd.com/read/28812968/jnk-pathway-in-osteoarthritis-pathological-and-therapeutic-aspects
#10
Hong-Xing Ge, Fu-Man Zou, Yan Li, An-Min Liu, Min Tu
CONTEXT: Osteoarthritis (OA) is a common chronic degenerative joint disease resulting in physical disability and reduced quality of life. Different biochemical signaling pathways are involved in the progression of OA, including the c-Jun NH2-terminal kinase (JNK) signal transduction pathway. OBJECTIVE: In this study, we have reviewed the recent updates on the association of JNK pathway with OA. METHODS: In this review, we have explored the databases like PubMed, Google Scholar, Medline, Scopus, etc...
October 2017: Journal of Receptor and Signal Transduction Research
https://www.readbyqxmd.com/read/28811491/high-fat-diet-induced-acceleration-of-osteoarthritis-is-associated-with-a-distinct-and-sustained-plasma-metabolite-signature
#11
Poulami Datta, Yue Zhang, Alexa Parousis, Anirudh Sharma, Evgeny Rossomacha, Helal Endisha, Brian Wu, Izabela Kacprzak, Nizar N Mahomed, Rajiv Gandhi, Jason S Rockel, Mohit Kapoor
Metabolic changes induced by high fat diet (HFD) that contribute to osteoarthritis (OA) are poorly understood. We investigated longitudinal changes to metabolites and their contribution to OA pathogenesis in response to HFD. HFD-fed mice exhibited acceleration of spontaneous age-related and surgically-induced OA compared to lean diet (LD)-fed mice. Using metabolomics, we identified that HFD-fed mice exhibited a distinct and sustained plasma metabolite signature rich in phosphatidylcholines (PC) and lysophosphatidylcholines (lysoPCs), even after resumption of normal chow diet...
August 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28810995/chondrocyte-viability-after-a-simulated-blast-exposure
#12
K Aaron Shaw, Peter C Johnson, David Williams, Steven D Zumbrun, Richard Topolski, Craig D Cameron
OBJECTIVES: The effects of blast exposure have gained increasing interest in the military medical community with their continued occurrence on the battlefield. The impact of the direct and indirect energy imparted from blasts to hollow viscera, as well as closed head injuries, have been well studied. However, the injury to articular cartilage has not been investigated, despite previous correlations regarding the development of osteoarthritis. The purpose of this study was to assess the degree of injury to articular chondrocytes after exposure to a simulated blast overpressure wave...
July 2017: Military Medicine
https://www.readbyqxmd.com/read/28810862/the-cytotoxic-mechanism-of-epigallocatechin-gallate-on-proliferative-hacat-keratinocytes
#13
Yu-Wen Chu, Shu-Ting Liu, Ya-Lan Yang, Shih-Ming Huang, Wei-Ming Wang
BACKGROUND: Epigallocatechin gallate (EGCG) is the major ingredient of sinecatechins ointment, approved for the treatment of external genital and perianal warts. However, the molecular mechanism for EGCG's effect on warts resulting from the human papillomavirus (HPV) infection of keratinocytes is not well understood. HPV may survive in proliferative keratinocytes and may be involved in cell cycle regulation and progression. The objective of this study was to investigate the mechanism underlying EGCG's treatment on external genital warts of HPV infection through the cultured keratinocyte cells from the HaCaT cell line...
August 15, 2017: Journal of Biomedical Science
https://www.readbyqxmd.com/read/28810001/deferoxamine-synergizes-with-transforming-growth-factor-%C3%AE-signaling-in-chondrogenesis
#14
Zheng Huang, Guangxu He, Yanke Huang
Osteoarthritis, also known as degenerative arthritis or degenerative joint disease, is an epidemic disease that affects millions of people worldwide. Despite extensive recent work on the cellular biology of osteoarthritis, the precise mechanisms involved are still poorly understood and there is no effective treatment for this disease. The role of transforming growth factor-beta (TGF-β) in promoting chondrogenesis and inducing the expression of cartilage-specific extracellular matrix molecules to form cartilage is well-established...
August 14, 2017: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/28808811/construction-and-biocompatibility-of-a-thin-type-i-ii-collagen-composite-scaffold
#15
Long Han, Zhong-Wen Zhang, Bo-He Wang, Zhen-Kun Wen
Articular cartilage injury is a common type of damage observed in clinical practice. A matrix-induced autologous chondrocyte implant was developed to repair articular cartilage as an advancement on the autologous chondrocyte implant procedure. Here, we establish a thin double layer of collagen as a novel and effective bioscaffold for the regeneration of cartilaginous lesions. We created a collagen membrane with double layers using a cover slip, a cover slip, and the collagen was then freeze-dried under vacuum...
August 14, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28808696/psychological-stress-alters-extracellular-matrix-metabolism-in-mandibular-condylar-cartilage
#16
Qiang Li, Fei Huang, Jia Liu, Yin Hua Zhao, Min Zhang, Yong Jin Chen
OBJECTIVE: To explore the effect of long-term stress on the temporomandibular joint (TMJ) condyle and its possible underlying mechanism. METHODS: A 12-week, chronic unpredictable mild stress (CUMS) model was used to induce long-term psychological stress in rats. Rats were randomly divided into control group (CONT), chronic unpredictable mild stress group (CUMS) and chronic unpredictable mild stress with fluoxetine treatment group (CUMS + DT) (n = 30 per group). A 5 mg/kg dose of fluoxetine was intraperitoneally injected daily 0...
2017: Chinese Journal of Dental Research
https://www.readbyqxmd.com/read/28807034/exosomes-from-embryonic-mesenchymal-stem-cells-alleviate-osteoarthritis-through-balancing-synthesis-and-degradation-of-cartilage-extracellular-matrix
#17
Yafei Wang, Dongsheng Yu, Zhiming Liu, Fang Zhou, Jun Dai, Bingbing Wu, Jing Zhou, Boon Chin Heng, Xiao Hui Zou, Hongwei Ouyang, Hua Liu
BACKGROUND: Mesenchymal stem cell therapy for osteoarthritis (OA) has been widely investigated, but the mechanisms are still unclear. Exosomes that serve as carriers of genetic information have been implicated in many diseases and are known to participate in many physiological processes. Here, we investigate the therapeutic potential of exosomes from human embryonic stem cell-induced mesenchymal stem cells (ESC-MSCs) in alleviating osteoarthritis (OA). METHODS: Exosomes were harvested from conditioned culture media of ESC-MSCs by a sequential centrifugation process...
August 14, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28806939/effect-of-electromagnetic-fields-on-human-osteoarthritic-and-non-osteoarthritic-chondrocytes
#18
Julia Isabelle Redeker, Bärbel Schmitt, Nele Pascale Grigull, Christian Braun, Andreas Büttner, Volkmar Jansson, Susanne Mayer-Wagner
BACKGROUND: Studies of the effects of electromagnetic fields (EMFs) on cartilaginous cells show a broad range of outcomes. However EMFs are not yet clinically applied as standard treatment of osteoarthritis, as EMF effects are showing varying outcomes in the literature. The aim of this study was to examine effects of EMFs (5 mT or 8 mT) on osteoarthritic (OA) and non-OA chondrocytes in order to investigate whether EMF effects are related to chondrocyte and EMF quality. METHODS: Pellets of human OA and non-OA chondrocytes were exposed to a sinusoidal 15 Hz EMF produced by a solenoid...
August 14, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28806392/kaempferol-alleviates-the-interleukin-1%C3%AE-induced-inflammation-in-rat-osteoarthritis-chondrocytes-via-suppression-of-nf-%C3%AE%C2%BAb
#19
Zhengling Zhuang, Guangqun Ye, Bin Huang
BACKGROUND This study was designed to examine the anti-inflammatory and anti-osteoarthritis (OA) effects of kaempferol in rat articular chondrocytes stimulated with interleukin-1β. MATERIAL AND METHODS Rat articular chondrocytes cultures were treated with interleukin-1β alone or with kaempferol (25, 50, 100, and 200 μM) and interleukin-1β. The effect of kaempferol on chondrocyte cells viability was measured by MTT assay. The effect on prostaglandin E2 (PGE2) and nitric oxide (NO) level were also assessed using the ELISA and Griess reagent, respectively, for kaempferol activity...
August 14, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/28805067/the-lncrna-nespas-is-associated-with-osteoarthritis-progression-and-serves-as-a-potential-new-prognostic-biomarker
#20
Sujung Park, Myeungsoo Lee, Churl-Hong Chun, Eun-Jung Jin
INTRODUCTION: In this article, we explored the hypothesis that the long noncoding RNA, Nespas, promotes osteoarthritis (OA) by supporting abnormal lipid metabolism in human chondrocytes. MATERIALS AND METHODS: Human articular chondrocytes from osteoarthritis patients were used and expression level of Nespas were determined by real-time polymerase chain reaction. Introduction of Nespas and Nespas-associated genes/miRNAs were performed by using a lentiviral system...
August 1, 2017: Cartilage
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