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https://www.readbyqxmd.com/read/29457374/proteasomal-function-is-impaired-in-human-osteoarthritic-chondrocytes-and-this-can-contribute-to-decreased-sox9-and-aggrecan
#1
Ramon L Serrano, Liang-Yu Chen, Martin K Lotz, Ru Liu-Bryan, Robert Terkeltaub
OBJECTIVE: Osteoarthritis (OA) chondrocytes have impaired autophagy, one arm of the proteostasis network that coordinates proteome and organelle quality control and degradation. Deficient proteostasis impacts differentiation and viability, and inflammatory processes in aging and disease. Studying OA chondrocytes, we assessed ubiquitin proteasome system proteasomal function. METHODS: We evaluated human knee cartilages by immunohistochemistry, and assessed proteasomal function, levels of proteasomal core subunits and chaperones, and autophagy in cultured chondrocytes...
February 18, 2018: Arthritis & Rheumatology
https://www.readbyqxmd.com/read/29456886/heterogenic-transplantation-of-bone-marrow-derived-rhesus-macaque-mesenchymal-stem-cells-ameliorates-liver-fibrosis-induced-by-carbon-tetrachloride-in-mouse
#2
Xufeng Fu, Bin Jiang, Bingrong Zheng, Yaping Yan, Junfeng Wang, Yanchao Duan, Shanshan Li, Li Yan, Hong Wang, Bingbing Chen, Xiongbo Sang, Weizhi Ji, Ren-He Xu, Wei Si
Liver fibrosis is a disease that causes high morbidity and has become a major health problem. Liver fibrosis can lead to the end stage of liver diseases (livercirrhosisand hepatocellularcarcinoma). Currently, liver transplantation is the only effective treatment for end-stage liver disease. However, the shortage of organ donors, high cost of medical surgery, immunological rejection and transplantation complications severely hamper liver transplantation therapy. Mesenchymal stem cells (MSCs) have been regarded as promising cells for clinical applications in stem cell therapy in the treatment of liver diseases due to their unique multipotent differentiation capacity, immunoregulation and paracrine effects...
2018: PeerJ
https://www.readbyqxmd.com/read/29456027/enhanced-chondrogenesis-differentiation-of-human-induced-pluripotent-stem-cells-by-microrna-140-and-transforming-growth-factor-beta-3-tgf%C3%AE-3
#3
Hossein Mahboudi, Masoud Soleimani, Seyed Ehsan Enderami, Mousa Kehtari, Abdolreza Ardeshirylajimi, Mohamad Eftekhary, Bahram Kazemi
Induced pluripotent stem cells (iPSCs) make an attractive source for regenerative medicine. The objective of our study was to establish a new method for differentiation of human iPSCs toward chondrocyte by overexpression of MicroRNA-140 (miR-140) and use of transforming growth factor beta 3 (TGFβ3) in high-cell density culture systems. We prepared vectors and then was used for recombinant Lenti virus production in HEK-293 cell. Transducted cells were selected and cultured in pellet culture system and were harvested after days 7, 14 and 21...
February 15, 2018: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/29455069/photo-crosslinkable-injectable-sericin-hydrogel-as-3d-biomimetic-extracellular-matrix-for-minimally-invasive-repairing-cartilage
#4
Chao Qi, Jia Liu, Yang Jin, Luming Xu, Guobin Wang, Zheng Wang, Lin Wang
Millions of patients worldwide suffer from cartilage injury and age/disease-related cartilage degeneration. However, cartilage, such as articular cartilage, is poor at self-regeneration. Current treatments are often invasive with limited efficacy. Developing minimal invasive strategies for effective cartilage repair is highly desired. Here, we report an injectable, photo-crosslinkable sericin hydrogel as a biomimetic extracellular matrix for minimal invasively repairing cartilage. Sericin was functionalized to be sericin methacryloyl (SerMA), which formed an in situ hydrogel upon UV light irradiation via photo-crosslinking...
February 9, 2018: Biomaterials
https://www.readbyqxmd.com/read/29454076/the-biological-basis-of-treating-jaw-discrepancies-an-interplay-of-mechanical-forces-and-skeletal-configuration
#5
REVIEW
Konstantinos Karamesinis, Efthimia K Basdra
Jaw discrepancies and malrelations affect a large proportion of the general population and their treatment is of utmost significance for individuals' health and quality of life. The aim of their therapy is the modification of aberrant jaw development mainly by targeting the growth potential of the mandibular condyle through its cartilage, and the architectural shape of alveolar bone through a suture type of structure, the periodontal ligament. This targeted treatment is achieved via external mechanical force application by using a wide variety of intraoral and extraoral appliances...
February 14, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29453775/effect-of-pparg-on-ages-induced-akt-mtor-signaling-associated-human-chondrocytes-autophagy
#6
Zhao-Jun Wang, Hai-Bin Zhang, Cheng Chen, Hao Huang, Jian-Xia Liang
Accumulation of advanced glycation end products (AGEs) in articular cartilage is thought to represent a major risk factor for osteoarthritis development. In this study we aimed to probe the role of AGEs in human chondrocytes and to determine the impact of the peroxisome proliferator-activated receptor-γ (PPARG) on AGEs-induced cell autophagy. Cell viability was measured after human chondrocytes were treated with different concentrations of AGEs with or without the PPARG inhibitor, T0070907, or agonist, pioglitazone...
February 17, 2018: Cell Biology International
https://www.readbyqxmd.com/read/29453700/reliability-of-cartilage-digestion-and-fda-eb-fluorescence-staining-for-the-detection-of-chondrocyte-viability-in-osteochondral-grafts
#7
Jianhong Qi, Famin Cao, Yunning Han, Di Xie, Hongqiang Song, Bin Chen, Lu Zhou
The purpose of this study is to evaluate the reliability of cartilage digestion and fluorescein diacetate-ethidium bromide (FDA-EB) fluorescence staining for the detection of chondrocyte viability in osteochondral grafts. Sixteen fresh osteochondral grafts were harvested from pig knee condyles, and the articular cartilage tissue was preserved. Each cartilage graft was cut into two 70-µm thick pieces and randomly allocated to Group A or Group B. The cell viability of Group A was detected using FDA-EB fluorescence staining of the digested cartilage, and the viability of Group B was detected with FDA-EB fluorescence staining of cartilage sections...
February 16, 2018: Cell and Tissue Banking
https://www.readbyqxmd.com/read/29452304/the-chondrocyte-channelome-a-narrative-review
#8
Ali Mobasheri, Csaba Matta, Ilona Uzielienè, Emma Budd, Pablo Martín-Vasallo, Eiva Bernotiene
Chondrocytes are the main cells in the extracellular matrix of articular cartilage and possess a highly differentiated phenotype that is the hallmark of the unique physiological functions of this specialised load-bearing connective tissue. The plasma membrane of articular chondrocytes contains a rich and diverse complement of membrane proteins, known as the membranome, which defines the cell surface phenotype of the cells. The membranome is a key target of pharmacological agents and is important for chondrocyte function...
February 13, 2018: Joint, Bone, Spine: Revue du Rhumatisme
https://www.readbyqxmd.com/read/29452287/dental-pulp-stem-cell-derived-chondrogenic-cells-demonstrate-differential-cell-motility-in-type-i-and-type-ii-collagen-hydrogels
#9
Li Yao, Nikol Flynn
BACKGROUND CONTEXT: Advances in the development of biomaterials and stem cell therapy provide a promising approach to regenerating degenerated discs. The normal nucleus pulposus (NP) cells exhibit the similar phenotype as chondrocytes. Because dental pulp stem cells (DPSCs) can be differentiated into chondrogenic cells, the DPSCs and DPSCs-derived chondrogenic cells encapsulated in type I and type II collagen hydrogels can potentially be transplanted into degenerated nucleus pulposus (NP) to repair damaged tissue...
February 13, 2018: Spine Journal: Official Journal of the North American Spine Society
https://www.readbyqxmd.com/read/29452246/bmp-signalling-driven-osteogenesis-is-critically-dependent-on-prdx-1-expression-mediated-maintenance-of-chondrocyte-prehypetrophy
#10
Yogesh Kumar, Tathagata Biswas, Gatha Thacker, Jitendra Kumar Kanaujiya, Sandeep Kumar, Anukampa Shukla, Kainat Khan, Sabyasachi Sanyal, Naibedya Chattopadhyay, Amitabha Bandyopadhyay, Arun Kumar Trivedi
During endochondral ossification, cartilage template is eventually replaced by bone. This process involves several well characterised, stereotypic, molecular and cellular changes in the cartilage primordia. These steps involve transition from resting to proliferative and then pre-hypertrophic to finally hypertrophic cartilage. BMP signaling is necessary and sufficient for osteogenesis. However, the specific step(s) of endochondral ossification in which BMP signaling plays an essential role is not yet known...
February 13, 2018: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/29450205/revision-surgery-after-cartilage-repair-data-from-the-german-cartilage-registry-knorpelregister-dgou
#11
Jan M Pestka, Nam H Luu, Norbert P Südkamp, Peter Angele, Gunther Spahn, Wolfgang Zinser, Philipp Niemeyer
Background: Various operative strategies have been introduced to restore the integrity of articular cartilage when injured. The frequency of revision surgery after cartilage regenerative surgery remains incompletely understood. Purpose/Hypothesis: The purpose of this study was to identify the reasons for revision surgery after cartilage regenerative surgery of the knee. We hypothesized that in a large patient cohort, revision rates would differ from those in the current literature...
February 2018: Orthopaedic Journal of Sports Medicine
https://www.readbyqxmd.com/read/29448253/lycorine-suppresses-endplate-chondrocyte-degeneration-and-prevents-intervertebral-disc-degeneration-by-inhibiting-nf-%C3%AE%C2%BAb-signalling-pathway
#12
Gangliang Wang, Kangmao Huang, Yangxin Dong, Shuai Chen, Jianfeng Zhang, Jiying Wang, Ziang Xie, Xianfeng Lin, Xiangqiang Fang, Shunwu Fan
BACKGROUND/AIMS: Cartilaginous endplate (CEP) degeneration is an important cause for intervertebral disc (IVD) degeneration that leads to low-back pain. The identification of compounds that may prevent CEP degeneration is of interest for the prevention of IVD degeneration. METHODS: Catabolic protease expression in the CEP of disc degeneration patients was first assessed. The toxicity, function and underlying mechanism of lycorine (LY) on CEP-derived chondrocytes degeneration were assessed in vitro by flow cytometry analysis and western blotting...
February 9, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29448248/lncrna-gas5-overexpression-reverses-lps-induced-inflammatory-injury-and-apoptosis-through-up-regulating-klf2-expression-in-atdc5-chondrocytes
#13
Feng Li, Jianguang Sun, Shilei Huang, Gao Su, Guofu Pi
BACKGROUND/AIMS: Osteoarthritis (OA) is the most frequently occurring joint disease and characterized by degeneration of cartilage. As the unique cell type in cartilage, chondrocytes play a crucial role during OA. Our study explored the influence of long non-coding RNA (lncRNA) growth arrest-specific transcript 5 (GAS5) on lipopolysaccharides (LPS)-induced injury in ATDC5 cells. METHODS: Cell viability, apoptosis and expression of inflammatory cytokines were all assessed to evaluate LPS-induce inflammatory injury...
February 9, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29447948/roles-of-ihh-signaling-in-chondroprogenitor-function-in-postnatal-condylar-cartilage
#14
Naito Kurio, Cheri Saunders, Till E Bechtold, Imad Salhab, Hyun-Duck Nah, Sayantani Sinha, Paul C Billings, Maurizio Pacifici, Eiki Koyama
Condylar articular cartilage in mouse temporomandibular joint develops from progenitor cells near the articulating surface that proliferate, undergo chondrogenesis and mature into hypertrophic chondrocytes. However, it remains unclear how these processes are regulated, particularly postnatally. Here we focused on the apical polymorphic layer rich in progenitors and asked whether the phenotype and fate of the cells require signaling by Indian hedgehog (Ihh) previously studied in developing long bones. In condyles in newborn mice, the apical polymorphic/progenitor cell layer was ~10 cell layer-thick and expressed the articular matrix marker Tenascin-C (Tn-C), and the underlying thick cell layer expressed Tn-C as well as the chondrogenic master regulator Sox9...
February 12, 2018: Matrix Biology: Journal of the International Society for Matrix Biology
https://www.readbyqxmd.com/read/29446757/3d-bioprinting-mesenchymal-stem-cell-laden-construct-with-core-shell-nanospheres-for-cartilage-tissue-engineering
#15
Wei Zhu, Haitao Cui, Benchaa Boualam, Fahed Masood, Erin Flynn, Raj Rao, Zhiyong Zhang, Lijie Grace Zhang
Cartilage tissue is prone to degradation and has little capacity for self-healing due to its avascularity. Tissue engineering, which provides artificial scaffolds to repair injured tissues, is a novel and promising strategy for cartilage repair. 3D bioprinting offers even greater potential for repairing degenerative tissue by simultaneously integrating living cells, biomaterials, and biological cues to provide a customized scaffold. With regard to cell selection, mesenchymal stem cells (MSCs) hold great capacity for differentiating into a variety of cell types, including chondrocytes, and could therefore be utilized as a cartilage cell source in 3D bioprinting...
February 15, 2018: Nanotechnology
https://www.readbyqxmd.com/read/29444976/foxo-transcription-factors-modulate-autophagy-and-proteoglycan-4-in-cartilage-homeostasis-and-osteoarthritis
#16
Tokio Matsuzaki, Oscar Alvarez-Garcia, Sho Mokuda, Keita Nagira, Merissa Olmer, Ramya Gamini, Kohei Miyata, Yukio Akasaki, Andrew I Su, Hiroshi Asahara, Martin K Lotz
Aging is a main risk factor for osteoarthritis (OA). FoxO transcription factors protect against cellular and organismal aging, and FoxO expression in cartilage is reduced with aging and in OA. To investigate the role of FoxO in cartilage, Col2Cre-FoxO1, 3, and 4 single knockout (KO) and triple KO mice (Col2Cre-TKO) were analyzed. Articular cartilage in Col2Cre-TKO and Col2Cre-FoxO1 KO mice was thicker than in control mice at 1 or 2 months of age. This was associated with increased proliferation of chondrocytes of Col2Cre-TKO mice in vivo and in vitro...
February 14, 2018: Science Translational Medicine
https://www.readbyqxmd.com/read/29444596/activation-of-the-cannabinoid-receptor-1-by-acea-suppresses-senescence-in-human-primary-chondrocytes-through-sirt1-activation
#17
Dawei Zhang, Gang Zhang, Zongyu Li, Bingsheng Li
Senescence of chondrocytes and cartilage degeneration induced by the proinflammatory cytokine interleukin-1β is associated with the pathogenesis of osteoarthritis. The cannabinoid receptor 1 has been involved in the pathological development of various diseases. Here, we evaluated whether activation of cannabinoid receptor 1 using its selective agonist arachidonyl-2-chloroethylamide had an influence on cellular senescence induced by interleukin-1βin human chondrocytes. Our findings demonstrate that agonist arachidonyl-2-chloroethylamidedecreased senescence-associated β-galactosidase activity and cell cycle arrest in the G0/G1 phase induced by interleukin-1β...
January 1, 2018: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/29443763/epibulbar-complex-cartilaginous-choristoma-a-distinctive-clinicopathological-case-series-and-literature-review
#18
Zongduan Zhang, Zhengwei Yang, Qintuo Pan, Xiaoyi Qin, Yuxuan Deng, Yuehong Cao
To newly describe the clinical and histopathological characteristics of epibulbar complex cartilaginous choristoma incidentally observed in a series of pterygium excision patients.Noncomparative case series with chart review of 8 patients.During a 4-year period, we identified 8 cases of conventional unilateral nasal subpterygial cartilaginous choristoma in 1799 pterygium patients and analyzed their clinicopathological features. The incidence rate of this entity is 0.44% in pterygium patients. All of the cartilaginous choristomas were buried deep in the caruncle, covered by the pterygium, and embedded in tenon facia tissue...
February 2018: Medicine (Baltimore)
https://www.readbyqxmd.com/read/29442026/upregulation-of-p63-inhibits-chondrocyte-autophagy-thereby-enhancing-the-malignant-progression-of-osteoarthritis
#19
Yufan Wangyang, Xin Zheng, Guang-Wang Liu, Dong-Ya Li, Yan-Bo Feng, Tong-Ya Guo, Chao Ma, Tao Wang
Loss of autophagy is suggested to play a key role in the progression of osteoarthritis (OA). P63 is a member of the P53 family, which is widely dysregulated in various tumors. However, the specific role of P63 in chondrocyte autophagy has never been fully understood. Here, the expression level of P63 in the articular cartilages of OA patients and chondrocytes treated with 3-MA was explored using western blot. Autophagy was determined using transmission electron microscopy and mRFP-GFP-LC3 assay. Fewer autophagic vesicles were identified in the articular cartilages of OA patients compared with that of normal control...
June 1, 2017: Die Pharmazie
https://www.readbyqxmd.com/read/29441992/microrna-320a-protects-against-osteoarthritis-cartilage-degeneration-by-regulating-the-expressions-of-bmi-1-and-runx2-in-chondrocytes
#20
Hao Peng, Du Liang, Baolin Li, Chizhang Liang, Wenhua Huang, Haibin Lin
Osteoarthritis (OA) is one of the most common chronic degenerative diseases characterized by deterioration of articular cartilage. Many studies have demonstrated the role of microRNAs (miRNAs) in OA, but the role of miR-320a in OA remains elusive. The aim of this study was to identify the protective role of miR-320a in OA cartilage degeneration by regulating the expression of BMI-1 and RUNX-2 proteins in chondrocytes. Normal and OA chondrocytes obtained from patients were cultured in vitro. The chondrocytes (both normal and OA) were transfected with miR-320a inhibitor to investigate the effects of miR-320a on chondrocyte proliferation, and to identify the miR-320a target proteins...
April 1, 2017: Die Pharmazie
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