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https://www.readbyqxmd.com/read/28655215/development-of-a-new-method-based-on-unmodified-gold-nanoparticles-and-peptide-nucleic-acids-for-detecting-bovine-viral-diarrhea-virus-rna
#1
Maryam Askaravi, Seyedeh Elham Rezatofighi, Saadat Rastegarzadeh, Masoud Reza Seifi Abad Shapouri
A simple colorimetric assay is presented for detecting bovine viral diarrhea virus (BVDV)-RNA based on aggregation of gold nanoparticles (AuNPs) in the presence of charge-neutral peptide nucleic acids (PNA). Free charge-neutral PNA oligomers tended to be adsorbed onto AuNPs and act as a coagulant, whereas hybridizing complementary RNA with PNA disrupted PNA-induced AuNP aggregation, and the NPs remained stable. However, non-complementary RNA did not have this effect, and PNA induced aggregation of the AuNPs that resulted in a color change of the reaction from red to blue...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28655201/unplanned-reoperation-after-craniotomy-for-tumor-a-national-surgical-quality-improvement-program-analysis
#2
Hormuzdiyar H Dasenbrock, Sandra C Yan, Vamsi Chavakula, William B Gormley, Timothy R Smith, Elizabeth B Claus, Ian F Dunn
BACKGROUND: Reoperation has been increasingly utilized as a metric evaluating quality of care. OBJECTIVE: To evaluate the rate of, reasons for, and predictors of unplanned reoperation after craniotomy for tumor in a nationally accrued population. METHODS: Patients who underwent cranial tumor resection were extracted from the prospective National Surgical Quality Improvement Program registry (2012-2014). Multivariate logistic regression examined predictors of unplanned cranial reoperation...
June 26, 2017: Neurosurgery
https://www.readbyqxmd.com/read/28655194/predicting-indirect-effects-of-predator-prey-interactions
#3
Sarah E Gilman
Predicting the effects of climate change on species and communities remains a pre-eminent challenge for biologists. Critical among this is understanding the indirect effects of climate change, which arise when the direct, physiological effects of climate on one species change the outcome of its interaction with a second species, altering the success of the second species. A diverse array of approaches to predicting indirect effects exists from mechanistic models, which attempt to build-up from physiological changes to ecological consequences, to ecological models that focus solely on the ecological scale...
June 23, 2017: Integrative and Comparative Biology
https://www.readbyqxmd.com/read/28655131/thrombospondin-i-concentrations-behavior-in-plasma-of-patients-with-ovarian-cancer
#4
Aneta Cymbaluk-Płoska, Anita Chudecka-Głaz, Ewa Pius-Sadowska, Bogusław Machaliński, Janusz Menkiszak
PURPOSE: To determine whether thrombospondin-1 might be used as a prognostic factor in ovarian cancer patients. METHOD: Ninety-six female subjects hospitalized in years 2011-2014 was included in the study. Transvaginal ultrasound examination was performed in all patients. In 39 cases of suspected ovarian cancer, CT scans were also performed. Each patient had been subjected to collection of a 5-mL blood sample before the laparoscopic procedure. Thrombospondin-1 concentrations were quantified in serum by multiplex fluorescent bead-based immunoassays (Luminex) at the Laboratory of the Department of General Pathology...
June 2, 2017: Cancer Biomarkers: Section A of Disease Markers
https://www.readbyqxmd.com/read/28654928/risk-stratification-of-patients-with-crohn-s-disease-a-retrospective-analysis-of-clinical-decision-making-and-its-impact-on-long-term-outcome
#5
Mahmoud Mosli, Hanadi Sabbahi, Hind Alyousef, Mada Abdulhaq, Afnan Hadadi, Emad Aljahdali, Hani Jawa, Salem Bazarah, Yousif Qari
BACKGROUND AND AIMS: Complications such as need for bowel resections and hospitalization due to Crohn's disease (CD) occur when disease activity persists due to ineffective therapy. Certain "high-risk" features require an early introduction of anti-tumor necrosis factor-α therapy to prevent such complications. We aim to evaluate the prevalence of "high-risk" features among a cohort of patients with CD and examine the association between discordance of early therapy with baseline risk stratification and disease outcome...
June 28, 2017: Digestive Diseases
https://www.readbyqxmd.com/read/28654692/heart-rate-variability-as-predictor-of-mortality-in-sepsis-a-prospective-cohort-study
#6
Fábio M de Castilho, Antonio Luiz P Ribeiro, José Luiz P da Silva, Vandack Nobre, Marcos R de Sousa
BACKGROUND: Sepsis is a serious medical condition with increasing prevalence and high mortality. The role of the autonomic nervous system in pathophysiology of sepsis has been increasingly researched. The objective of this study is to evaluate the Heart rate variability (HRV) as a predictor of mortality in septic patients. METHODS: This was a prospective cohort of patients diagnosed with sepsis. Patient recruitment was carried out at ICU in tertiary university hospital between March 2012 and February 2014...
2017: PloS One
https://www.readbyqxmd.com/read/28654561/influenza-associated-encephalitis-encephalopathy-identified-by-the-australian-childhood-encephalitis-study-2013-2015
#7
Philip N Britton, Russell C Dale, Christopher C Blyth, Kristine Macartney, Nigel Crawford, Helen Marshall, Julia Clark, Elizabeth Elliott, Richard I Webster, Allen C Cheng, Robert Booy, Cheryl A Jones
BACKGROUND: Influenza associated encephalitis/encephalopathy (IAE) is an important cause of acute encephalitis syndrome in children. IAE includes a series of clinico-radiologic syndromes or acute encephalopathy syndromes that have been infrequently reported outside East Asia. We aimed to describe case of IAE identified by the Australian Childhood Encephalitis study. METHODS: Children ≤14 years of age with suspected encephalitis were prospectively identified in 5 hospitals in Australia...
June 24, 2017: Pediatric Infectious Disease Journal
https://www.readbyqxmd.com/read/28654468/bullous-pemphigoid-use-of-c4d-immunofluorescent-staining-in-a-case-with-repeated-negative-conventional-direct-immunofluorescence-studies
#8
Sarah S Kassaby, Alexander Hicks, Stuart Leicht, George A Youngberg
Direct immunofluorescence (DIF) using frozen section material from a fresh/preserved perilesional biopsy is the gold standard for the immunopathologic diagnosis of bullous pemphigoid (BP). DIF in BP shows linear dermoepidermal junction (DEJ) staining for C3, with or without staining for IgG. In some situations, only a formalin-fixed lesional biopsy is obtained (with no fresh/preserved perilesional biopsy for DIF). In this setting, paraffin section C4d immunohistochemistry has proven to be diagnostically useful, demonstrating linear DEJ positivity for C4d...
June 20, 2017: American Journal of Dermatopathology
https://www.readbyqxmd.com/read/28654443/application-and-interpretation-of-histocompatibility-data-in-thoracic-heart-and-lung-transplantation
#9
Kelly H Schlendorf, Ashish S Shah
PURPOSE OF REVIEW: The purpose of the review is to update our current understanding and utilization of immunogenetic tools in heart and lung transplant. RECENT FINDINGS: Increasingly, complex patients have been managed perioperatively for heart and lung transplant using a variety of tests and techniques. Recent treatment regimens and listing strategies have exploited recent laboratory advances. However, the better characterization has led to an even more complex description of sensitized heart and lung candidates...
June 24, 2017: Current Opinion in Organ Transplantation
https://www.readbyqxmd.com/read/28654291/photocatalytic-e%C3%A2-z-isomerization-of-polarized-alkenes-inspired-by-the-visual-cycle-mechanistic-dichotomy-and-origin-of-selectivity
#10
Jan B Metternich, Denis Gennadievich Artiukhin, Mareike C Holland, Maximilian von Bremen-Kühne, Johannes Neugebauer, Ryan Gilmour
Iteratively executed with exquisite spatial and temporal control, the selective isomerization of polarized alkenes underpins a plethora of complex biological processes ranging from natural product biosynthesis through to the mammalian visual cycle. However, Nature's proficiency conceals the inherent difficulties in replicating this contra-thermodynamic transformation in the laboratory. Recently, we disclosed the first highly Z-selective isomerization of polarized alkenes, employing the cinnamoyl chromophore as a retinal surrogate under UV-irradiation (402 nm) with (-)-riboflavin (vitamin B2) as an inexpensive, organic photocatalyst (J...
June 27, 2017: Journal of Organic Chemistry
https://www.readbyqxmd.com/read/28654180/hydrometeorology-organizes-intra-annual-patterns-of-tree-growth-across-time-space-and-species-in-a-montane-watershed
#11
Justin Martin, Nathaniel Looker, Zachary Hoylman, Kelsey Jencso, Jia Hu
Tree radial growth is often systematically limited by water availability, as is evident in tree ring records. However, the physiological nature of observed tree growth limitation is often uncertain outside of the laboratory. To further explore the physiology of water limitation, we observed intra-annual growth rates of four conifer species using point dendrometers and microcores, and coupled these data to observations of water potential, soil moisture, and vapor pressure deficit over 2 yr in the Northern Rocky Mountains, USA...
June 27, 2017: New Phytologist
https://www.readbyqxmd.com/read/28654123/chemical-physics-of-electroactive-materials-the-oft-overlooked-faces-of-electrochemistry
#12
Zinaida A Kostiuchenko, Piotr J Glazer, Eduardo Mendes, Serge G Lemay
Electroactive materials and their applications are enjoying renewed attention, in no small part motivated by the advent of nanoscale tools for their preparation and study. While the fundamentals of charge and mass transport in electrolytes on this scale are by and large well understood, their interplay can have subtle manifestations in the more complex situations typical of, for example, integrated microfluidics-based applications. In particular, the role of faradaic processes is often overlooked or, at best, purposefully suppressed via experimental design...
June 27, 2017: Faraday Discussions
https://www.readbyqxmd.com/read/28654076/a-method-to-test-the-efficacy-of-handwashing-for-the-removal-of-emerging-infectious-pathogens
#13
Marlene K Wolfe, Daniele S Lantagne
Handwashing is widely recommended to prevent infectious disease transmission. However, little comparable evidence exists on the efficacy of handwashing methods in general. Additionally, little evidence exists comparing handwashing methods to determine which are most efficacious at removing infectious pathogens. Research is needed to provide evidence for the different approaches to handwashing that may be employed during infectious disease outbreaks. Here, a laboratory method to assess the efficacy of handwashing methods at removing microorganisms from hands and their persistence in rinse water is described...
June 7, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28654042/a-unified-methodological-framework-for-vestibular-schwannoma-research
#14
Lukas D Landegger, Jessica E Sagers, Sonam Dilwali, Takeshi Fujita, Mehmet I Sahin, Konstantina M Stankovic
Vestibular schwannomas are the most common neoplasms of the cerebellopontine angle, making up 6-8% percent of all intracranial growths. Though these tumors cause sensorineural hearing loss in up to 95% of affected individuals, the molecular mechanisms underlying this hearing loss remain elusive. This article outlines the steps established in our laboratory to facilitate the collection and processing of various primary human tissue samples for downstream research applications integral to the study of vestibular schwannomas...
June 20, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28654029/measuring-g-protein-coupled-receptor-signaling-via-radio-labeled-gtp-binding
#15
Chirag Vasavda, Nicholas W Zaccor, Paul C Scherer, Charlotte J Sumner, Solomon H Snyder
G-Protein-Coupled Receptors (GPCRs) are a large family of transmembrane receptors that play critical roles in normal cellular physiology and constitute a major pharmacological target for multiple indications, including analgesia, blood pressure regulation, and the treatment of psychiatric disease. Upon ligand binding, GPCRs catalyze the activation of intracellular G-proteins by stimulating the incorporation of guanosine triphosphate (GTP). Activated G-proteins then stimulate signaling pathways that elicit cellular responses...
June 9, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28654002/integration-of-sensors-controllers-and-instruments-using-a-novel-opc-architecture
#16
Isaías González, Antonio José Calderón, Antonio Javier Barragán, José Manuel Andújar
The interconnection between sensors, controllers and instruments through a communication network plays a vital role in the performance and effectiveness of a control system. Since its inception in the 90s, the Object Linking and Embedding for Process Control (OPC) protocol has provided open connectivity for monitoring and automation systems. It has been widely used in several environments such as industrial facilities, building and energy automation, engineering education and many others. This paper presents a novel OPC-based architecture to implement automation systems devoted to R&D and educational activities...
June 27, 2017: Sensors
https://www.readbyqxmd.com/read/28653944/association-between-serum-25-oh-vitamin-d-and-metabolic-disturbances-in-polycystic-ovary-syndrome
#17
M A Keshavarz, S Moradi, Z Emami, F Rohani
BACKGROUND: To assess the relationship between serum 25(OH) vitamin D levels and metabolic parameters together with androgenic hormone levels in women with polycystic ovary syndrome (PCOS). METHODS: This is a single-centre study from the Endocrinology Institute at Firouzgar Hospital in Iran. Seventy-three women aged 15-45 years were recruited from May 2013 to December 2013. Serum 25(OH) vitamin D level, other laboratory biomarkers and anthropometric indexes were measured...
June 2017: Netherlands Journal of Medicine
https://www.readbyqxmd.com/read/28653868/knee-frontal-plane-biomechanics-in-adults-with-or-without-bone-marrow-edema-like-lesions-after-anterior-cruciate-ligament-injury
#18
Abbey C Thomas, Riann M Palmieri-Smith
CONTEXT:   Lateral subchondral bone bruises (BBs) occur frequently with anterior cruciate ligament (ACL) injuries. These BBs are associated with pain during weight bearing, leading individuals to increase medial tibiofemoral loading to alleviate pain laterally. Increased medial tibiofemoral loading may precipitate the development or progression of posttraumatic osteoarthritis; however, no in vivo biomechanical data exist to confirm that lateral BBs increase medial tibiofemoral loading as measured by the external knee-adduction moment (KAM)...
June 2, 2017: Journal of Athletic Training
https://www.readbyqxmd.com/read/28653867/association-between-the-single-assessment-numeric-evaluation-and-the-western-ontario-and-mcmaster-universities-osteoarthritis-index
#19
Brian Pietrosimone, Brittney A Luc, Austin Duncan, Susan A Saliba, Joseph M Hart, Christopher D Ingersoll
CONTEXT:   Patient-reported outcomes (PROs) evaluate how patients describe symptoms as well as level of physical function or quality of life. The Western Ontario and McMaster Universities Osteoarthritis (WOMAC) Index is one of the most common PROs used to assess disability in patients with knee osteoarthritis (OA), yet the Single Assessment Numeric Evaluation (SANE) is a single-question instrument that may improve the efficiency associated with the measurement of patient function. OBJECTIVE:   To determine the associations between (1) SANEFunction and the physical dysfunction subsection of the WOMAC index (WOMACDysfunction) before rehabilitation and after a 4-week therapeutic exercise intervention as well as (2) the percentage change in SANEFunction and WOMACDysfunction in people with knee OA after 4 weeks of therapeutic exercise...
June 2, 2017: Journal of Athletic Training
https://www.readbyqxmd.com/read/28653865/sagittal-plane-knee-moment-during-gait-and-knee-cartilage-thickness
#20
Randy J Schmitz, David Harrison, Hsin-Min Wang, Sandra J Shultz
CONTEXT:   Understanding the factors associated with thicker cartilage in a healthy population is important when developing strategies aimed at minimizing the cartilage thinning associated with knee osteoarthritis progression. Thicker articular cartilage is commonly thought to be healthier cartilage, but whether the sagittal-plane biomechanics important to gait are related to cartilage thickness is unknown. OBJECTIVE:   To determine the relationship of a weight-bearing region of the medial femoral condyle's cartilage thickness to sagittal gait biomechanics in healthy individuals...
June 2, 2017: Journal of Athletic Training
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