keyword
MENU ▼
Read by QxMD icon Read
search

Clinical diagnostics

keyword
https://www.readbyqxmd.com/read/29156849/interest-of-integrins-targeting-in-glioblastoma-according-to-tumor-heterogeneity-and-cancer-stem-cell-paradigm-an-update
#1
REVIEW
Laure Malric, Sylvie Monferran, Julia Gilhodes, Sabrina Boyrie, Perrine Dahan, Nicolas Skuli, Julie Sesen, Thomas Filleron, Aline Kowalski-Chauvel, Elizabeth Cohen-Jonathan Moyal, Christine Toulas, Anthony Lemarié
Glioblastomas are malignant brain tumors with dismal prognosis despite standard treatment with surgery and radio/chemotherapy. These tumors are defined by an important cellular heterogeneity and notably contain a particular subpopulation of Glioblastoma-initiating cells, which recapitulate the heterogeneity of the original Glioblastoma. In order to classify these heterogeneous tumors, genomic profiling has also been undertaken to classify these heterogeneous tumors into several subtypes. Current research focuses on developing therapies, which could take into account this cellular and genomic heterogeneity...
October 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/29156844/a-three-microrna-signature-for-lung-squamous-cell-carcinoma-diagnosis-in-chinese-male-patients
#2
Lan Zhang, Xia Shan, Jun Wang, Jun Zhu, Zebo Huang, Huo Zhang, Xin Zhou, Wenfang Cheng, Yongqian Shu, Wei Zhu, Ping Liu
Various studies have demonstrated the diagnostic value of microRNA (miRNA) for lung cancer, but miRNA signatures varied between different subtypes. Whether serum miRNAs could be used as biomarkers in lung squamous cell carcinoma (SCC) remains unknown. Using quantitative real-time polymerase chain reaction (qRT-PCR) based Exiqon panel, 38 differentially expressed miRNAs were identified from 3 male lung SCC pool samples and 1 normal control (NC) pool in the initial screening phase. After the training (24 SCC VS...
October 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/29156832/associations-of-high-altitude-polycythemia-with-polymorphisms-in-epas1-itga6-and-erbb4-in-chinese-han-and-tibetan-populations
#3
Yiduo Zhao, Zhiying Zhang, Lijun Liu, Yao Zhang, Xiaowei Fan, Lifeng Ma, Jing Li, Yuan Zhang, Haijin He, Longli Kang
High altitude polycythemia (HAPC) is a common chronic disease at high altitude, which is characterized by excessive erythrocytosis (females, hemoglobin ≥ 190 g/L; males, hemoglobin ≥ 210 g/L). It is the most common disease in chronic mountain sickness casued primarily by persistent arterial hypoxia and ventilatory impairment. However, the disease is still unmanageable and related molecular mechanisms remain largely unclear. This study aims to explore the genetic basis of HAPC in the Chinese Han and Tibetan populations...
October 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/29156783/isolation-of-circulating-tumor-cells-from-pancreatic-cancer-by-automated-filtration
#4
Nora Brychta, Michael Drosch, Christiane Driemel, Johannes C Fischer, Rui P Neves, Irene Esposito, Wolfram Knoefel, Birte Möhlendick, Claudia Hille, Antje Stresemann, Thomas Krahn, Matthias U Kassack, Nikolas H Stoecklein, Oliver von Ahsen
It is now widely recognized that the isolation of circulating tumor cells based on cell surface markers might be hindered by variability in their protein expression. Especially in pancreatic cancer, isolation based only on EpCAM expression has produced very diverse results. Methods that are independent of surface markers and therefore independent of phenotypical changes in the circulating cells might increase CTC recovery also in pancreatic cancer. We compared an EpCAM-dependent (IsoFlux) and a size-dependent (automated Siemens Healthineers filtration device) isolation method for the enrichment of pancreatic cancer CTCs...
October 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/29156741/screening-epitopes-on-systemic-lupus-erythematosus-autoantigens-with-a-peptide-array
#5
Lin Wang, Chenjun Hao, Yongqiu Deng, Yanbo Liu, Shiliang Hu, Yangang Peng, Manna He, Jinhu Fu, Ming Liu, Jia Chen, Xiaoming Chen
Systemic lupus erythematosus (SLE) is a common autoimmune disease. Many autoantibodies are closely associated with SLE. However, the specific epitopes recognized and bound by these autoantibodies are still unclear. This study screened the binding epitopes of SLE-related autoantibodies using a high-throughput screening method. Epitope prediction on 12 SLE-related autoantigens was performed using the Immune Epitope Database and Analysis Resource (IEDB) software. The predicted epitopes were synthesized into peptides and developed into a peptide array...
October 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/29156734/pax3d-mrna-over-2-76-copies-%C3%A2%C2%B5l-in-the-bloodstream-predicts-cutaneous-malignant-melanoma-relapse
#6
Chiara Autilio, Carmela Paolillo, Maria Michela Lavieri, Krizia Pocino, Elisa De Paolis, Enrico Di Stasio, Paolo Marchetti, Cappellini Antonini Gian Carlo, Ettore Capoluongo
Objective: The aim of this study was to evaluate if our molecular algorithm, based on tumor circulating transcripts, may predict relapse risk in cutaneous malignant melanoma (CMM). Results: The multi-marker panel was able to differentiate patients with CMM from HC with high diagnostic sensitivity and specificity, especially for MITF-m and TGFB2 (91-100%) whose levels decreased during follow-up of recurrence-free patients, and remained stable in the case of relapse...
October 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/29156720/novel-strategies-of-raman-imaging-for-brain-tumor-research
#7
Imiela Anna, Polis Bartosz, Polis Lech, Abramczyk Halina
Raman diagnostics and imaging have been shown to be an effective tool for the analysis and discrimination of human brain tumors from normal structures. Raman spectroscopic methods have potential to be applied in clinical practice as they allow for identification of tumor margins during surgery. In this study, we investigate medulloblastoma (grade IV WHO) (n= 5), low-grade astrocytoma (grades I-II WHO) (n =4), ependymoma (n=3) and metastatic brain tumors (n= 1) and the tissue from the negative margins used as normal controls...
October 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/29156712/radiolabelled-polymeric-iga-from-biodistribution-to-a-new-molecular-imaging-tool-in-colorectal-cancer-lung-metastases
#8
Helene Carpenet, Armelle Cuvillier, Aurélie Perraud, Ophélie Martin, Gaël Champier, Marie-Odile Jauberteau, Jacques Monteil, Isabelle Quelven
By radiolabelling monomeric (m) and polymeric (p) IgA with technetium 99m ((99m)Tc), this study assessed IgA biodistribution and tumour-targeting potency. IgA directed against carcinoembryonic antigen (CEA), a colorectal cancer marker, was selected to involve IgA mucosal tropism. Ig was radiolabelled with (99m)Tc-tricarbonyl after derivatisation by 2-iminothiolane. (99m)Tc-IgA was evaluated by in vitro analysis. The biodistributions of radiolabelled anti-CEA mIgA, pIgA and IgG were compared in normal mice. Anti-CEA pIgA tumour uptake was studied in mice bearing the WiDr caecal orthotopic graft...
October 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/29156364/pattern-recognition-of-magnetic-resonance-imaging-based-gray-matter-volume-measurements-classifies-bipolar-disorder-and-major-depressive-disorder
#9
Harry Rubin-Falcone, Francesca Zanderigo, Binod Thapa-Chhetry, Martin Lan, Jeffrey M Miller, M Elizabeth Sublette, Maria A Oquendo, David J Hellerstein, Patrick J McGrath, Johnathan W Stewart, J John Mann
BACKGROUND: Bipolar Disorder (BD) cannot be reliably distinguished from Major Depressive Disorder (MDD) until the first manic or hypomanic episode. Consequently, many patients with BD are treated with antidepressants without mood stabilizers, a strategy that is often ineffective and carries a risk of inducing a manic episode. We previously reported reduced cortical thickness in right precuneus, right caudal middle-frontal cortex and left inferior parietal cortex in BD compared with MDD...
November 13, 2017: Journal of Affective Disorders
https://www.readbyqxmd.com/read/29156361/non-suicidal-self-injury-in-referred-adolescents-with-mood-disorders-and-its-association-with-cyclothymic-hypersensitive-temperament
#10
Gabriele Masi, Annarita Milone, Anna Rita Montesanto, Elena Valente, Simone Pisano
BACKGROUND: Non suicidal self-injuries (NSSIs) are deliberate self-harm behaviors without suicidal intent, usually starting in adolescence, with increasing rates of occurrence both in epidemiological and clinical samples. Several studies associated cyclothymic-hypersensitive temperament (CHT) with self-harm behaviors and suicidal risk. Aim of this study is to explore the association between NSSIs and CHT in a clinical sample of adolescents. We hypothesized that CHT may differentiate NSSI from non-NSSI adolescents with mood disorders, when other psychopathological features are controlled for...
November 13, 2017: Journal of Affective Disorders
https://www.readbyqxmd.com/read/29156315/genus-and-species-level-identification-of-dermatophyte-fungi-by-surface-enhanced-raman-spectroscopy
#11
Evelin Witkowska, Tomasz Jagielski, Agnieszka Kamińska
This paper demonstrates that surface-enhanced Raman spectroscopy (SERS) coupled with principal component analysis (PCA) can serve as a fast and reliable technique for detection and identification of dermatophyte fungi at both genus and species level. Dermatophyte infections are the most common mycotic diseases worldwide, affecting a quarter of the human population. Currently, there is no optimal method for detection and identification of fungal diseases, as each has certain limitations. Here, for the first time, we have achieved with a high accuracy, differentiation of dermatophytes representing three major genera, i...
November 3, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/29156294/phospholipase-a1-based-cross-reactivity-among-venoms-of-clinically-relevant-hymenoptera-from-neotropical-and-temperate-regions
#12
Amilcar Perez-Riverol, Luís Gustavo Romani Fernandes, Alexis Musacchio Lasa, José Roberto Aparecido Dos Santos-Pinto, Débora Moitinho Abram, Gabriel Hideki Izuka Moraes, Frederic Jabs, Michaela Miehe, Henning Seismman, Mario Sergio Palma, Ricardo de Lima Zollner, Edzard Spillner, Márcia Regina Brochetto-Braga
Molecular cross-reactivity caused by allergen homology or cross-reactive carbohydrate determinants (CCDs) is a major challenge for diagnosis and immunotherapy of insect venom allergy. Venom phospholipases A1 (PLA1s) are classical, mostly non-glycosylated wasp and ant allergens that provide diagnostic benefit for differentiation of genuine sensitizations from cross-reactivity. As CCD-free molecules, venom PLA1s are not causative for CCD-based cross-reactivity. Little is known however about the protein-based cross-reactivity of PLA1 within vespid species...
November 17, 2017: Molecular Immunology
https://www.readbyqxmd.com/read/29156147/electromagnetic-navigational-bronchoscopy-versus-ct-guided-percutaneous-sampling-of-peripheral-indeterminate-pulmonary-nodules-a-cohort-study
#13
Kavita M Bhatt, Yasmeen K Tandon, Ruffin Graham, Charles T Lau, Jason K Lempel, Joseph T Azok, Peter J Mazzone, Erika Schneider, Nancy A Obuchowski, Michael A Bolen
Purpose To compare the diagnostic yield and complication rates of electromagnetic navigational bronchoscopic (ENB)-guided and computed tomography (CT)-guided percutaneous tissue sampling of lung nodules. Materials and Methods Retrospectively identified were 149 patients sampled percutaneously with CT guidance and 146 patients who underwent ENB with transbronchial biopsy of a lung lesion between 2013 and 2015. Clinical data, incidence of complications, and nodule pathologic analyses were assessed through electronic medical record review...
November 20, 2017: Radiology
https://www.readbyqxmd.com/read/29156078/correlation-between-clinical-examination-and-diagnostic-imaging-in-type-ii-peters-anomaly
#14
Ta C Chang, Daniela Reyes-Capo, Kara M Cavuoto
No abstract text is available yet for this article.
November 1, 2017: Journal of Pediatric Ophthalmology and Strabismus
https://www.readbyqxmd.com/read/29156044/characteristics-prevention-and-management-of-hepatitis-b-virus-hbv-reactivation-in-hbv-infected-patients-who-require-immunosuppressive-therapy
#15
Jonggi Choi, Young-Suk Lim
Hepatitis B virus (HBV) reactivation becomes a challenging issue with increasing use of immunosuppressive agents and cytotoxic chemotherapy for varied medical conditions, including cancer. The spectrum of HBV reactivation in the setting of immunosuppression may vary from asymptomatic reactivation to liver failure leading to death. HBV reactivation can hamper the course of planned therapies and diminish the effects of therapies; thus, it adversely affects the prognosis of the original disease and the survival of the patients...
November 16, 2017: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29155891/the-burden-of-gynecomastia-among-men-on-antiretroviral-therapy-in-zomba-malawi
#16
Victor Singano, Alemayehu Amberbir, Daniela Garone, Christopher Kandionamaso, Jack Msonko, Monique van Lettow, Kondwani Kalima, Yamikani Mataka, Gift Kawalazira, Gabriel Mateyu, Aunex Kwekwesa, Alfred Matengeni, Joep J van Oosterhout
BACKGROUND: Many Africans who are on life-saving ART face challenges from a variety of toxicities. After the introduction of a standardized first-line efavirenz-containing ART regimen, reports of gynecomastia appeared in Malawian popular media, however data on the prevalence and risk factors of gynecomastia from Africa are lacking. METHODS: We conducted a cross-sectional study in males ≥18 years registered on ART at the HIV clinic in Zomba Central Hospital. Men who reported to have ever experienced breast or nipple enlargement received a standard questionnaire and underwent physical examination...
2017: PloS One
https://www.readbyqxmd.com/read/29155823/development-and-preliminary-evaluation-of-a-multiplexed-amplification-and-next-generation-sequencing-method-for-viral-hemorrhagic-fever-diagnostics
#17
Annika Brinkmann, Koray Ergünay, Aleksandar Radonić, Zeliha Kocak Tufan, Cristina Domingo, Andreas Nitsche
BACKGROUND: We describe the development and evaluation of a novel method for targeted amplification and Next Generation Sequencing (NGS)-based identification of viral hemorrhagic fever (VHF) agents and assess the feasibility of this approach in diagnostics. METHODOLOGY: An ultrahigh-multiplex panel was designed with primers to amplify all known variants of VHF-associated viruses and relevant controls. The performance of the panel was evaluated via serially quantified nucleic acids from Yellow fever virus, Rift Valley fever virus, Crimean-Congo hemorrhagic fever (CCHF) virus, Ebola virus, Junin virus and Chikungunya virus in a semiconductor-based sequencing platform...
November 20, 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29155793/metabolic-support-of-excised-living-brain-tissues-during-magnetic-resonance-microscopy-acquisition
#18
Jeremy J Flint, Kannan Menon, Brian Hansen, John Forder, Stephen J Blackband
This protocol describes the procedures necessary to support normal metabolic functions of acute brain slice preparations during the collection of magnetic resonance (MR) microscopy data. While it is possible to perform MR collections on living, excised mammalian tissue, such experiments have traditionally been constrained by resolution limits and are thus incapable of visualizing tissue microstructure. Conversely, MR protocols that did achieve microscopic image resolution required the use of fixed samples to accommodate the need for static, unchanging conditions over lengthy scan times...
October 18, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29155639/when-machines-think-radiology-s-next-frontier
#19
Keith J Dreyer, J Raymond Geis
Artificial intelligence (AI), machine learning, and deep learning are terms now seen frequently, all of which refer to computer algorithms that change as they are exposed to more data. Many of these algorithms are surprisingly good at recognizing objects in images. The combination of large amounts of machine-consumable digital data, increased and cheaper computing power, and increasingly sophisticated statistical models combine to enable machines to find patterns in data in ways that are not only cost-effective but also potentially beyond humans' abilities...
December 2017: Radiology
https://www.readbyqxmd.com/read/29155636/hepatic-imaging-in-neonates-and-young-infants-state-of-the-art
#20
Stephanie B Shamir, Jessica Kurian, Debora Kogan-Liberman, Benjamin H Taragin
Neonatal liver disease is an important source of morbidity in the pediatric population. The manifestation of liver disease in young infants may be different than in older patients, and there are a number of diagnoses that are unique to this age group. Familiarity with these entities is important as imaging plays a key role in the diagnostic workup, and prompt diagnosis is necessary to prevent complications. This article reviews the spectrum of liver pathologies that can manifest in the first 6 months of life and is intended to educate the general radiologist who may be faced with interpretation of neonatal liver imaging...
December 2017: Radiology
keyword
keyword
13742
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"