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Abril Ortiz-Matamoros, Clorinda Arias
Wnt signaling plays an important role in the adult brain function and its dysregulation has been implicated in some neurodegenerative pathways. Despite the functional role of the Wnt signaling in adult neural circuits, there is currently no evidence regarding the relationships between exogenously Wnt signaling activation or inhibition and hippocampal structural changes in vivo. Thus, we analyzed the effect of the chronic infusion of Wnt agonists, Wnt7a and Wnt5a, and antagonist, Dkk-1, on different markers of plasticity such as neuronal MAP-2, Tau, synapse number and morphology, and behavioral changes...
March 13, 2018: Brain Research Bulletin
Meng Wu, Mengya Chen, Yubo Ma, Jiajia Yang, Renfang Han, Yaping Yuan, Xingxing Hu, Mengmeng Wang, Xu Zhang, Shengqian Xu, Rui Liu, Guangming Jiang, Jianhua Xu, Zongwen Shuai, Yanfeng Zou, Guixia Pan, Faming Pan
BACKGROUND: Current findings regarding serum Dickkopf-1 (DKK-1) concentration in ankylosing spondylitis (AS) have proven inconsistent. This meta-analysis was performed to provide a better understanding of serum DKK-1 and AS. METHODS: Online electronic databases were used to retrieve all relevant articles published before November 2017. The pooled standardized mean difference (SMD) with 95% confidence interval (CI) was calculated by the random-effect model. RESULTS: 23 studies, containing 1348 AS patients and 909 healthy controls, were included in this meta-analysis...
March 12, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
Klara Janjić, Barbara Cvikl, Christoph Kurzmann, Andreas Moritz, Hermann Agis
BACKGROUND: To understand the responses of the dental pulp to hypoxia is of high relevance for regenerative endodontics and dental traumatology. Here, we aimed to reveal the effects of hypoxia and the hypoxia mimetic agent L-mimosine (L-MIM) on the production of sclerostin (SOST) and dickkopf-1 (DKK-1) in human dental pulp-derived cells (DPC). METHODS: DPC in monolayer, spheroid and tooth slice cultures were treated with L-MIM or hypoxia. Resazurin-based toxicity and MTT assays were performed to determine cell viability...
March 9, 2018: BMC Oral Health
Bushra Parveen, Manjari Tripathi, Divya Vohora
Long-term anti-epileptic drug (AED) therapy compromises bone health. Though vitamin-D deficiency is proposed to be involved, it alone is not held responsible. This accounts for investigating other mechanisms in bone accrual. Recent studies have shown modulation of inhibitors of wnt pathway, sclerostin and dickkopf-1 (DKK-1), in glucocorticoids-induced osteoporosis. We investigated whether AED monotherapy modulates wnt inhibitors in Indian women with epilepsy. Women of age > 20-40 years with the diagnosis of epilepsy and receiving AEDs (carbamazepine, valproate and levetiracetam) for at least a year were enrolled...
March 5, 2018: Basic & Clinical Pharmacology & Toxicology
Francesca Maria Trovato, Paola Castrogiovanni, Marta Anna Szychlinska, Francesco Purrello, Giuseppe Musumeci
BACKGROUND: The metabolic syndrome is associated with sarcopenia. Decreased serum levels of Vitamin D (VitD) and insulin-like growth factor (IGF)-1 and their mutual relationship were also reported. We aimed to evaluate whether different dietary profiles, containing or not VitD, may exert different effects on muscle molecular morphology. METHODS: Twenty-eight male rats were fed for 10 weeks in order to detect early defects induced by different dietary regimens: regular diet (R); regular diet with vitamin D supplementation (R-DS) and regular diet with vitamin D restriction (R-DR); high-fat butter-based diets (HFB-DS and HFB-DR) with 41% energy from fat; high-fat extra-virgin olive oil-based diets (HFEVO-DS and HFEVO-DR) with 41% energy from fat...
February 17, 2018: Nutrients
Jieping Li, Wenlin Gong, Xiaoxue Li, Ruirong Wan, Fengzhen Mo, Zhenghua Zhang, Panpan Huang, Zixi Hu, Zongqiang Lai, Xiaoling Lu, Yongxiang Zhao
Hepatocellular carcinoma (HCC) is one of the most common cancers around the world. Multiple etiologic factors such as virus and environment can lead to HCC. It is a challenge for us to successfully detect early HCC due to the lack of effective characterized and specific biomarkers. However, if the early diagnosis is successfully realized, it provides crucial chance for HCC patients to receive effective treatment as early as possible. Dickkopf-1 (DKK-1) is a secretary glycoprotein, which negatively regulates Wnt pathway through binding to surface receptors LRP5/6 and Kremen 1/2...
August 1, 2018: Journal of Nanoscience and Nanotechnology
Jadwiga Ambroszkiewicz, Magdalena Chełchowska, Katarzyna Szamotulska, Grażyna Rowicka, Witold Klemarczyk, Małgorzata Strucińska, Joanna Gajewska
Vegetarian diets contain many beneficial properties as well as carry a risk of inadequate intakes of several nutrients important to bone health. The aim of the study was to evaluate serum levels of bone metabolism markers and to analyze the relationships between biochemical bone markers and anthropometric parameters in children on vegetarian and omnivorous diets. The study included 70 prepubertal children on a lacto-ovo-vegetarian diet and 60 omnivorous children. Body composition, bone mineral content (BMC), and bone mineral density (BMD) were assessed by dual-energy X-ray absorptiometry...
February 7, 2018: Nutrients
Lu-Hong Cong, Shi-Yu Du, Yi-Na Wu, Ying Liu, Tao Li, Hui Wang, Gang Li, Jun Duan
We evaluated the effects of Klotho on pulmonary vascular remodeling and cell proliferation and apoptosis in rat models with PM2.5-induced pulmonary arterial hypertension (PAH) via the Wnt signaling pathway. After establishing rat models of PM2.5-induced PAH, these Sprague-Dawley male rats were randomized into control and model groups. Cells extracted from the model rats were sub-categorized into different groups. Activation of Wnt/β-catenin signaling transcription factor was detected by a TOPFlash/FOPFlash assay...
January 30, 2018: Journal of Cellular Biochemistry
Fatma Gossiel, Jessica R Scott, Margaret A Paggiosi, Kim E Naylor, Eugene V McCloskey, Nicola F A Peel, Jennifer S Walsh, Richard Eastell
Context: Treatment of postmenopausal osteoporosis with teriparatide (PTH 1-34) increases bone formation and improves bone microarchitecture. A possible modulator of this mechanism of action is periostin. In vitro experiments have shown that periostin may regulate osteoblast differentiation and bone formation through Wnt signaling. Periostin secretion is increased by PTH in preclinical models, but the effect of teriparatide treatment of postmenopausal osteoporosis on periostin is not currently known...
January 22, 2018: Journal of Clinical Endocrinology and Metabolism
Manreet Kaur Padwal, Genyang Cheng, Limin Liu, Felix J Boivin, Azim Gangji, Kenneth Scott Brimble, Darren Bridgewater, Peter J Margetts
The WNT signaling pathway is involved in wound healing and fibrosis. We evaluated the WNT signaling pathway in peritoneal membrane injury. We assessed WNT1 protein expression in the peritoneal effluents of 54 stable peritoneal dialysis (PD) patients and WNT-related gene expression in ex vivo mesothelial cell cultures from 21 PD patients. In a transforming growth factor beta (TGFB) mediated animal model of peritoneal fibrosis, we evaluated regulation of the WNT pathway and the effect of WNT inhibition on peritoneal fibrosis and angiogenesis...
January 24, 2018: American Journal of Physiology. Renal Physiology
Cheng Xue, Ling-Ling Hong, Jun-Sheng Lin, Xiang-Yang Yao, Ding-Hui Wu, Xiao-Ping Lin, Jia-Min Zhang, Xiao-Bin Zhang, Yi-Ming Zeng
Benign airway stenosis is a clinical challenge because of recurrent granulation tissues. Our previous study proved that a Chinese drug, β-elemene, could effectively inhibit the growth of fibroblasts cultured from hyperplastic human airway granulation tissues, which could slow down the progression of this disease. The purpose of this study is to find out the mechanism. We cultured fibroblasts from normal human airway tissues and human airway granulation tissues. These cells were cultured with 160 ug/ml NS, different doses of β-elemene, or 10 ng/ml canonical Wnt/β-catenin pathway inhibitor (DKK-1)...
January 22, 2018: Bioscience Reports
Rong Mu, Jun Liang, Lingyun Sun, Zhuoli Zhang, Xiangyuan Liu, Cibo Huang, Ping Zhu, Xiaoxia Zuo, Jieruo Gu, Xiangpei Li, Xingfu Li, Yi Liu, Ping Feng, Zhanguo Li
AIM: To investigate the efficacy and safety of technetium-99 conjugated with methylene diphosphonate (99 Tc-MDP, Yunke Pharmaceutical industry) in the treatment of rheumatoid arthritis (RA). METHODS: A total of 120 patients with active RA were randomly divided into three groups: Group A (receiving oral meloxicam tablets); Group B (receiving intravenous drip of 99 TC-MDP); Group C (receiving combination treatment of intravenous drip of 99 Tc-MDP and oral meloxicam tablets)...
January 2018: International Journal of Rheumatic Diseases
G Brunetti, G Grugni, L Piacente, M Delvecchio, A Ventura, P Giordano, M Grano, G D'Amato, D Laforgia, A Crinò, M F Faienza
We tested the hypothesis that the levels of bone remodeling mediators may be altered in Prader-Willi syndrome (PWS). We assessed RANKL, OPG, sclerostin, DKK-1 serum levels, and bone metabolism markers in 12 PWS children (7.8 ± 4.3 years), 14 PWS adults (29.5 ± 7.2 years), and 31 healthy controls matched for sex and age. Instrumental parameters of bone mineral density (BMD) were also evaluated. Lumbar spine BMD Z-scores were reduced in PWS children (P < 0.01), reaching osteopenic levels in PWS adults...
January 20, 2018: Calcified Tissue International
Alessia Mastrodonato, Saviana Antonella Barbati, Lucia Leone, Claudia Colussi, Katia Gironi, Marco Rinaudo, Roberto Piacentini, Christine A Denny, Claudio Grassi
Exposure to extremely low-frequency electromagnetic fields (ELFEF) influences the expression of key target genes controlling adult neurogenesis and modulates hippocampus-dependent memory. Here, we assayed whether ELFEF stimulation affects olfactory memory by modulating neurogenesis in the subventricular zone (SVZ) of the lateral ventricle, and investigated the underlying molecular mechanisms. We found that 30 days after the completion of an ELFEF stimulation protocol (1 mT; 50 Hz; 3.5 h/day for 12 days), mice showed enhanced olfactory memory and increased SVZ neurogenesis...
January 10, 2018: Scientific Reports
Athanasios D Anastasilakis, Polyzois Makras, Maria Pikilidou, Symeon Tournis, Konstantinos Makris, Ilias Bisbinas, Olga Tsave, John G Yovos, Maria P Yavropoulou
Context: Expression of microRNAs (miRs) related to bone metabolism in the serum maybe affected by anti-osteoporotic treatment. Objective: To investigate the effect of two anti-osteoporotic agents with opposite effects on bone metabolism on microRNA expression profile in the serum. Design: Observational, open label, non-randomized clinical trial. Setting: The outpatient clinics for Metabolic Bone Diseases of 424 General Military Hospital, Thessaloniki, Greece...
December 22, 2017: Journal of Clinical Endocrinology and Metabolism
Jiapeng Bao, Chiyuan Ma, Jisheng Ran, Yan Xiong, Shigui Yan, Lidong Wu
To investigate the inflammatory effect of Interleukin 18 (IL-18) on rat chondrocytes and the involvement of Wnt/β-catenin and Hedgehog pathways, the mRNA and protein level of matix-degrading enzymes (MMP-2, 3, 9,13 and aggrecanses) and chondrocyte-specific proteins (Collagen II and aggrecan) were evaluated by qRT-PCR and Western blot, and key protein level of Wnt/β-catenin and Hedgehog pathways including β-catenin, GSK-3β, DKK-1, IHH, SHH, and Gli-2 were evaluated by Western blot. Dickkopf-1 (DKK-1) and Cyclopamine were used as antagonist of Wnt/β-catenin and Hedgehog pathways to perform pathway inhibition tests...
December 15, 2017: Oncotarget
Madeleine Johansson, Fabrizio Ricci, Nay Aung, Richard Sutton, Olle Melander, Artur Fedorowski
Orthostatic hypotension (OH) has been linked with higher incidence of cardiovascular disease, but little is known about the mechanisms behind this association. We aimed to identify cardiovascular disease biomarkers associated with OH through a proteomic profiling approach. Seven hundred seventy-eight patients with unexplained syncope or orthostatic intolerance underwent head-up tilt test and supine blood samples. Of these, 220 met diagnostic criteria of OH, and 179 demonstrated normal hemodynamic response during head-up tilt test...
March 2018: Hypertension
Jian Wu, Ji-Feng Huang, Xing-Xing Qin, Feng Hu, Zhao-Fei Chen, Yong Zheng, Yan-Xi Liu, Xian-Hua Cai
OBJECTIVE: Platelet-rich plasma (PRP) has been reported to alleviate degenerative pathological damage to joint cartilage. This study aimed to investigate the effect of PRP on Wnt/β-catenin signaling in rabbit chondrocytes. METHODS: Using 3-month-old New Zealand white rabbits, PRP was prepared from venous blood, and chondrocytes were cultured from knee joint cartilage and identified by staining for type II collagen and proteoglycan. The effects of PRP on chondrocyte viability were measured...
December 29, 2017: International Immunopharmacology
Roberto Franceschi, Silvia Longhi, Vittoria Cauvin, Angelo Fassio, Giuseppe Gallo, Fiorenzo Lupi, Petra Reinstadler, Antonio Fanolla, Davide Gatti, Giorgio Radetti
Adults with Type 1 diabetes mellitus show a high risk of bone fracture, probably as a consequence of a decreased bone mass and microarchitectural bone alterations. The aim of the study was to investigate the potential negative effects of type 1 diabetes on bone geometry, quality, and bone markers in a group of children and adolescents. 96 children, mean age 10.5 ± 3.1 years, agreed to participate to the study. Bone geometry was evaluated on digitalized X-rays at the level of the 2nd metacarpal bone. The following parameters were investigated and expressed as SDS: outer diameter (D), inner diameter (d), cortical area (CA), and medullary area (MA)...
December 30, 2017: Calcified Tissue International
Li Cheng, Yan Zhao, Di Qi, Wen Li, Daoxin Wang
T helper cell 17 (Th17), one type of CD4+ T cell, plays an important role in regulating the acute lung injury (ALI) inflammatory response. Recent studies showed that Wnt/β-catenin pathway could modulate the differentiation and the function of CD4+ T cell. However, whether Wnt/β-catenin could regulate the differentiation and function of Th17 in the development and progress of ALI induced by lipopolysaccharide (LPS) is still unknown. To test this, we used dickkopf1 (Dkk-1) to block the Wnt/β-catenin pathway and LiCl to activate the Wnt/β-catenin pathway by instillation to the murine model of ALI...
December 12, 2017: Biochemical and Biophysical Research Communications
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