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https://www.readbyqxmd.com/read/27849597/correction-for-ren-et-al-lrp-6-is-a-coreceptor-for-multiple-fibrogenic-signaling-pathways-in-pericytes-and-myofibroblasts-that-are-inhibited-by-dkk-1
#1
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No abstract text is available yet for this article.
November 14, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/27779642/impact-of-vitamin-e-blended-uhmwpe-wear-particles-on-the-osseous-microenvironment-in-polyethylene-particle-induced-osteolysis
#2
Carl Neuerburg, Theresa Loer, Lena Mittlmeier, Christina Polan, Zsuzsanna Farkas, Lesca Miriam Holdt, Sandra Utzschneider, Jens Schwiesau, Thomas M Grupp, Wolfgang Böcker, Attila Aszodi, Christian Wedemeyer, Christian Kammerlander
Aseptic loosening mediated by wear particle-induced osteolysis (PIO) remains the major cause of implant loosening in endoprosthetic surgery. The development of new vitamin E (α-tocopherol)-blended ultra-high molecular weight polyethylene (VE-UHMWPE) with increased oxidation resistance and improved mechanical properties has raised hopes. Furthermore, regenerative approaches may be opened, as vitamin E supplementation has shown neuroprotective characteristics mediated via calcitonin gene-related peptide (CGRP), which is known to affect bone remodeling in PIO...
October 18, 2016: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/27776430/baicalein-promotes-angiogenesis-and-odontoblastic-differentiation-via-the-bmp-and-wnt-pathways-in-human-dental-pulp-cells
#3
Sang-Im Lee, Sun-Young Kim, Kyung-Ran Park, Eun-Cheol Kim
Baicalein is an active flavonoid extracted from the root of Scutellaria baicalensis that has anticancer and anti-inflammatory properties; its effects on osteoblastic and angiogenic potential are controversial. The aim of this study was to investigate the effects of baicalein on odontoblastic differentiation and angiogenesis and the underlying mechanism in human dental pulp cells (HDPCs). Baicalein (1-10[Formula: see text][Formula: see text]M) had no cytotoxic effects and promoted alkaline phosphatase (ALP) activity, mineralization assayed by Alizarin Red-S staining, and the mRNA expression of marker genes, in a concentration-dependent manner...
October 25, 2016: American Journal of Chinese Medicine
https://www.readbyqxmd.com/read/27769713/androgens-modify-wnt-agonists-antagonists-expression-balance-in-dermal-papilla-cells-preventing-hair-follicle-stem-cell-differentiation-in-androgenetic-alopecia
#4
Gustavo José Leirós, Julieta María Ceruti, María Lía Castellanos, Ana Gabriela Kusinsky, María Eugenia Balañá
In androgenetic alopecia, androgens impair dermal papilla-induced hair follicle stem cell (HFSC) differentiation inhibiting Wnt signaling. Wnt agonists/antagonists balance was analyzed after dihydrotestosterone (DHT) stimulation in androgen-sensitive dermal papilla cells (DPC) cultured as spheroids or monolayer. In both culture conditions, DHT stimulation downregulated Wnt5a and Wnt10b mRNA while the Wnt antagonist Dkk-1 was upregulated. Notably, tissue architecture of DPC-spheroids lowers Dkk-1 and enhances Wnt agonists' basal expression; probably contributing to DPC inductivity...
October 18, 2016: Molecular and Cellular Endocrinology
https://www.readbyqxmd.com/read/27732331/high-sclerostin-and-dickkopf-1-dkk-1-serum-levels-in-children-and-adolescents-with-type-1-diabetes-mellitus
#5
Maria Felicia Faienza, Annamaria Ventura, Maurizio Delvecchio, Anna Fusillo, Laura Piacente, Gabriella Aceto, Graziana Colaianni, Silvia Colucci, Luciano Cavallo, Maria Grano, Giacomina Brunetti
CONTEXT: Childhood type 1 diabetes (T1DM) is associated with decreased bone mass. Sclerostin and dickkopf-1 (DKK-1) are Wnt inhibitors which regulate bone formation. OBJECTIVE: To evaluate sclerostin and DKK-1 levels in TD1M children and to analyze the influence of the glycaemic control on bone health. DESIGN AND SETTING: Cross-sectional study conducted at a clinical research center. Partecipants: One hundred and six T1DM subjects (12.2 ± 4 years), 66 on multiple daily injections (MDI) and 40 on continuous subcutaneous infusion of insulin (CSII), and 80 controls...
October 12, 2016: Journal of Clinical Endocrinology and Metabolism
https://www.readbyqxmd.com/read/27709225/increased-circulating-resistin-is-associated-with-insulin-resistance-oxidative-stress-and-platelet-activation-in-type-2-diabetes-mellitus
#6
Francesca Santilli, Rossella Liani, Patrizia Di Fulvio, Gloria Formoso, Paola Simeone, Romina Tripaldi, Thor Ueland, Pål Aukrust, Giovanni Davì
Resistin is an adipokine that promotes inflammation and insulin resistance by targeting several cells including platelets. We hypothesised that in type 2 diabetes (T2DM), resistin may foster in vivo oxidative stress, thromboxane-dependent platelet activation and platelet-derived inflammatory proteins release, key determinants of atherothrombosis. A cross-sectional comparison of circulating resistin, sCD40L, as a marker of platelet-mediated inflammation, asymmetric dimethylarginine (ADMA), endothelial dysfunction marker, Dickkopf (DKK)-1, reflecting the inflammatory interaction between platelets and endothelial cells, and urinary 8-iso-PGF2α and 11-dehydro-TxB2, reflecting in vivo lipid peroxidation and platelet activation, respectively, was performed between 79 T2DM patients and 30 healthy subjects...
November 30, 2016: Thrombosis and Haemostasis
https://www.readbyqxmd.com/read/27673573/osteocyte-physiology-and-response-to-fluid-shear-stress-are-impaired-following-exposure-to-cobalt-and-chromium-implications-for-bone-health-following-joint-replacement
#7
Karan M Shah, Peter Orton, Nick Mani, Jeremy Mark Wilkinson, Alison Gartland
The effects of metal ion exposure on osteocytes, the most abundant cell type in bone and responsible for coordinating bone remodeling, remain unclear. However, several studies have previously shown that exposure to cobalt (Co(2+) ) and chromium (Cr(3+) ), at concentrations equivalent to those found clinically, affect osteoblast and osteoclast survival and function. In this study, we tested the hypothesis that metal ions would similarly impair the normal physiology of osteocytes. The survival, dendritic morphology, and response to fluid shear stress of the mature osteocyte-like cell-line MLO-Y4 following exposure to clinically relevant concentrations and combinations of Co and Cr ions were measured in 2D-culture...
September 27, 2016: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://www.readbyqxmd.com/read/27670695/the-role-of-dickkopf-1-in-joint-remodeling-and-fibrosis-a-link-connecting-spondyloarthropathies-and-scleroderma
#8
Kalliopi Klavdianou, Stamatis-Nick Liossis, Lazaros Sakkas, Dimitrios Daoussis
BACKGROUND: Dickkopf-1 (Dkk-1) is a soluble inhibitor of the canonical Wnt pathway, which plays critical roles in embryonic development. Evidence suggests that this molecule regulates several aspects of both bone biology and fibrosis. OBJECTIVES: To provide an overview of our current knowledge of the role of Dkk-1 in joint remodeling and fibrosis. METHODS: We performed an electronic search (Medline) using the following key words: Dickkopf-1 (or Dkk-1), new bone formation, joint remodeling, ankylosing spondylitis, systemic sclerosis (or scleroderma), and fibrosis, supplemented by a manual search of references from retrieved articles...
August 23, 2016: Seminars in Arthritis and Rheumatism
https://www.readbyqxmd.com/read/27668000/real-time-analysis-of-endogenous-wnt-signalling-in-3d-mesenchymal-stromal-cells
#9
Fatima Saleh, Alice Carstairs, S Leah Etheridge, Paul Genever
Wnt signalling has been implicated in the regulation of stem cell self-renewal and differentiation; however, the majority of in vitro studies are carried out using monolayer 2D culture techniques. Here, we used mesenchymal stromal cell (MSC) EGFP reporter lines responsive to Wnt pathway activation in a 3D spheroid culture system to mimic better the in vivo environment. Endogenous Wnt signalling was then investigated under basal conditions and when MSCs were induced to undergo osteogenic and adipogenic differentiation...
2016: Stem Cells International
https://www.readbyqxmd.com/read/27664561/dosage-effects-of-extracorporeal-shockwave-therapy-in-early-hip-necrosis
#10
Ching-Jen Wang, Chung-Cheng Huang, Hon-Kan Yip, Ya-Ju Yang
BACKGROUND: This study investigated the effects of different dosages of extracorporeal shockwave therapy (ESWT) in early osteonecrosis of the femoral head (ONFH). MATERIALS AND METHODS: Thirty-three patients (42 hips) were randomly divided into three groups. Group A (10 patients with 16 hips) received 2000 impulses of ESWT at 24 Kv to the affected hip. Group B (11 patients with 14 hips) and Group C (12 patients with 12 hips) received 4000 and 6000 impulses of ESWT respectively...
September 21, 2016: International Journal of Surgery
https://www.readbyqxmd.com/read/27616299/scalable-differentiation-of-human-ipscs-in-a-multicellular-spheroid-based-3d-culture-into-hepatocyte-like-cells-through-direct-wnt-%C3%AE-catenin-pathway-inhibition
#11
Giuseppe Pettinato, Rajesh Ramanathan, Robert A Fisher, Martin J Mangino, Ning Zhang, Xuejun Wen
Treatment of acute liver failure by cell transplantation is hindered by a shortage of human hepatocytes. Current protocols for hepatic differentiation of human induced pluripotent stem cells (hiPSCs) result in low yields, cellular heterogeneity, and limited scalability. In the present study, we have developed a novel multicellular spheroid-based hepatic differentiation protocol starting from embryoid bodies of hiPSCs (hiPSC-EBs) for robust mass production of human hepatocyte-like cells (HLCs) using two novel inhibitors of the Wnt pathway...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27611941/nts-ntr1-co-expression-enhances-epithelial-to-mesenchymal-transition-and-promotes-tumor-metastasis-by-activating-the-wnt-%C3%AE-catenin-signaling-pathway-in-hepatocellular-carcinoma
#12
Yingnan Ye, Xinxin Long, Lijie Zhang, Jieying Chen, Pengpeng Liu, Hui Li, Feng Wei, Wenwen Yu, Xiubao Ren, Jinpu Yu
Neurotensin (NTS) is a neuropeptide distributed in central nervous and digestive systems. In this study, the significant association between ectopic NTS expression and tumor invasion was confirmed in hepatocellular carcinoma (HCC). In primary HCC tissues, the NTS and neurotensin receptor 1 (NTR1) co-expression (NTS+NTR1+) is a poor prognostic factor correlated with aggressive biological behaviors and poor clinical prognosis. Enhanced epithelial-to-mesenchymal transition (EMT) features, including decreased E-cadherin, increased β-catenin translocation and N-cadherin expression, were identified in NTS+NTR1+ HCC tissues...
September 6, 2016: Oncotarget
https://www.readbyqxmd.com/read/27586146/chemotherapy-induced-dkk-1-expression-by-primary-human-mesenchymal-stem-cells-is-p53-dependent
#13
Ian Hare, Rebecca Evans, James Fortney, Blake Moses, Debbie Piktel, William Slone, Laura F Gibson
Mesenchymal stem cells (MSCs) are abundant throughout the body and regulate signaling within tumor microenvironments. Wnt signaling is an extrinsically regulated pathway that has been shown to regulate tumorigenesis in many types of cancer. After evaluating a panel of Wnt activating and inhibiting molecules, we show that primary human MSCs increase the expression of Dkk-1, an inhibitor of Wnt signaling, into the extracellular environment following chemotherapy exposure in a p53-dependent manner. Dkk-1 has been shown to promote tumor growth in several models of malignancy, suggesting that MSC-derived Dkk-1 could counteract the intent of cytotoxic chemotherapy, and that pharmacologic inhibition of Dkk-1 in patients receiving chemotherapy treatment for certain malignancies may be warranted...
October 2016: Medical Oncology
https://www.readbyqxmd.com/read/27578583/-tenascin-c-knockdown-suppresses-osteoblast-differentiation-and-promotes-osteoporosis-in-mice-by-inhibiting-wnt-signaling
#14
Yu Chen, Zi-Feng Chen, Fan He
OBJECTIVE: To understand the mechanism by which tenascin-C regulates osteoblast differentiation and the role of tenascin-C in osteoporosis. METHODS: Tenascin-C protein expression in femoral spongy bone of mice with or without osteoporosis was analyzed using Western blotting. In MC3T3-E1 osteoblasts with or without tenascin-C depletion by a specific siRNA targeting tenascin-C, alkaline phosphatase activity and Dickkopf-1 (DKK-1) expression were determined using quantitative RT-PCR and Western blotting, and the transcriptional activity of Wnt signaling pathway was analyzed using a luciferase reporter assay...
August 20, 2016: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
https://www.readbyqxmd.com/read/27573735/high-serum-levels-of-sclerostin-and-dickkopf-1-are-associated-with-acute-ischaemic-stroke
#15
Xin-Wei He, En Wang, Yu-Yan Bao, Feng Wang, Ming Zhu, Xiao-Fei Hu, Xiao-Ping Jin
BACKGROUND AND AIMS: Sclerostin and Dickkopf-1 (Dkk-1) are potent antagonists of Wnt signalling and might therefore play important roles in cardiovascular disease. We investigated whether serum sclerostin and Dkk-1 levels are associated with acute ischaemic stroke and specific stroke subtypes. METHODS: Serum levels of sclerostin and Dkk-1 were measured by ELISA on day 1 and on day 6 after stroke in 62 patients with large artery atherosclerotic (LAA) stroke, on day 1 after stroke in 62 age- and gender-matched patients with small-artery occlusion (SAO) stroke and on admission in 62 healthy controls...
October 2016: Atherosclerosis
https://www.readbyqxmd.com/read/27548498/leukocyte-beta-catenin-expression-is-disturbed-in-systemic-lupus-erythematosus
#16
Jacob J Orme, Yong Du, Kamala Vanarsa, Tianfu Wu, Anne B Satterthwaite, Chandra Mohan
Wnt/β-catenin signaling is relatively understudied in immunity and autoimmunity. β-catenin blocks inflammatory mediators and favors tolerogenic dendritic cell (DC) phenotypes. We show here that leukocytes from lupus-prone mice and SLE patients express diminished β-catenin transcriptional activity, particularly in myeloid cells, although other leukocytes revealed similar trends. Serum levels of DKK-1, an inhibitor under transcriptional control of Wnt/β-catenin, were also decreased in lupus-prone mice. Surprisingly, however, preemptive deletion of β-catenin from macrophages appears to have no effect on lupus development, even in mice with varying genetic loads for lupus...
2016: PloS One
https://www.readbyqxmd.com/read/27468227/thymoquinone-subdues-tumor-growth-and-potentiates-the-chemopreventive-effect-of-5-fluorouracil-on-the-early-stages-of-colorectal-carcinogenesis-in-rats
#17
Osama Adnan Kensara, Adel Galal El-Shemi, Amr Mohamed Mohamed, Bassem Refaat, Shakir Idris, Jawwad Ahmad
Colorectal cancer (CRC) is one of the most prevalent cancers and has a high mortality rate. Insensitivity and the limited therapeutic efficacy of its standard chemotherapeutic drug, 5-fluorouracil (5-FU), represents an important challenge in CRC treatment. The robust antitumor properties of thymoquinone (TQ), the main bioactive constituent of Nigella sativa, have recently been demonstrated on different cancers. We investigated whether TQ could potentiate the chemopreventive effect of 5-FU to eradicate the early stages of CRC and elucidated its underlying mechanisms...
2016: Drug Design, Development and Therapy
https://www.readbyqxmd.com/read/27450649/impaired-osteogenic-differentiation-associated-with-connexin43-microrna-206-in-steroid-induced-avascular-necrosis-of-the-femoral-head
#18
Gang Liu, Gaobin Luo, Zhandong Bo, Xiaonan Liang, Jie Huang, Donghui Li
Connexin(Cx)43 and microRNA(miR)-206 play an important role in osteogenesis. However, their role in steroid-induced femoral head osteonecrosis (SANFH) is still ambiguous. The present study aimed to establish a rabbit model and investigate osteogenesis in steroid-induced femoral head osteonecrosis occurring via Cx43/miR-206 and the changes of Wnt/β-catenin signal pathway-related proteins. A total of 72 adult New Zealand white rabbits were divided randomly into a model group (Group A) and a control group (Group B) of 36 rabbits each...
August 2016: Experimental and Molecular Pathology
https://www.readbyqxmd.com/read/27435781/-effect-of-parathyroid-hormone-1-34-on-expression-of-matrix-gla-protein-and-wnt-%C3%AE-catenin-signaling-pathways-in-mg63-cell-lines
#19
Ya-Li Hu, Jie Zhang, Liu-Chen Fu, Ya Yang
OBJECTIVE: To observe the effect of parathyroid hormone (PTH)(1-34) on the expression of matrix Gla protein (MGP) and Wnt/β-catenin signaling pathway and elucidate the possible molecular mechanism of PTH (1-34) in the prevention and treatment of osteoporosis. METHODS: MG63 cells treated with PTH (1-34) at 10(-9), 10(-8), and 10(-7) mol/L, alone or in combination with Wnt/β-catenin signaling pathway inhibitors DKK-1 (200 ng/ml) were examined for mRNA and protein expressions related with Wnt/β-catenin signaling with real-time PCR and Western blotting...
June 20, 2016: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
https://www.readbyqxmd.com/read/27397144/astragaloside-i-stimulates-osteoblast-differentiation-through-the-wnt-%C3%AE-catenin-signaling-pathway
#20
Xun Cheng, Biaofang Wei, Lijuan Sun, Xiaofang Hu, Jichao Liang, Yong Chen
Astragaloside I (As-I), one of the main active ingredients in Astragalus membranaceus, is believed to have osteogenic properties, but this hypothesis has not been investigated in detail. In the present work, the As-I-induced osteogenic effects and its underlying mechanism were studied in MC3T3-E1 cells. The results indicated that the cellular levels of ALP and extracellular matrix calcium increased in a dose-dependent manner by As-I. To clarify the mechanisms involved in this process, the effect of As-I on the key osteogenic-related genes was investigated...
July 10, 2016: Phytotherapy Research: PTR
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