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https://www.readbyqxmd.com/read/29912411/multiple-glutathione-s-transferase-genes-in-heortia-vitessoides-lepidoptera-crambidae-identification-and-expression-patterns
#1
Jie Cheng, Chun-Yan Wang, Zi-Hao Lyu, Tong Lin
To elucidate the role of glutathione S-transferases (GSTs) in Heortia vitessoides Moore (Lepidoptera: Crambidae), one of the most destructive defoliating pests in Aquilaria sinensis (Lour.) Gilg (Thymelaeaceae) forests, 16 GST cDNAs were identified in the transcriptome of adult H. vitessoides. All cDNAs included a complete open reading frame and were designated HvGSTd1-HvGSTu2. A phylogenetic analysis showed that the 16 HvGSTs were classified into seven different cytosolic classes; three in delta, two in epsilon, three in omega, three in sigma, one in theta, two in zeta, and two in unclassified...
May 1, 2018: Journal of Insect Science
https://www.readbyqxmd.com/read/29707112/full-in-frame-exon-3-skipping-of-brca2-confers-high-risk-of-breast-and-or-ovarian-cancer
#2
Sandrine M Caputo, Mélanie Léone, Francesca Damiola, Asa Ehlen, Aura Carreira, Pascaline Gaidrat, Alexandra Martins, Rita D Brandão, Ana Peixoto, Ana Vega, Claude Houdayer, Capucine Delnatte, Myriam Bronner, Danièle Muller, Laurent Castera, Marine Guillaud-Bataille, Inge Søkilde, Nancy Uhrhammer, Sophie Demontety, Hélène Tubeuf, Gaïa Castelain, Uffe Birk Jensen, Ambre Petitalot, Sophie Krieger, Cédrick Lefol, Virginie Moncoutier, Nadia Boutry-Kryza, Henriette Roed Nielsen, Olga Sinilnikova, Dominique Stoppa-Lyonnet, Amanda B Spurdle, Manuel R Teixeira, Florence Coulet, Mads Thomassen, Etienne Rouleau
Germline pathogenic variants in the BRCA2 gene are associated with a cumulative high risk of breast/ovarian cancer. Several BRCA2 variants result in complete loss of the exon-3 at the transcript level. The pathogenicity of these variants and the functional impact of loss of exon 3 have yet to be established. As a collaboration of the COVAR clinical trial group (France), and the ENIGMA consortium for investigating breast cancer gene variants, this study evaluated 8 BRCA2 variants resulting in complete deletion of exon 3...
April 3, 2018: Oncotarget
https://www.readbyqxmd.com/read/29679256/biomechanical-features-of-six-design-of-the-delta-external-fixator-for-treating-pilon-fracture-a-finite-element-study
#3
Muhammad Hanif Ramlee, Mohd Ayub Sulong, Evelyn Garcia-Nieto, Daniel Angure Penaranda, Antonio Ros Felip, Mohammed Rafiq Abdul Kadir
Pilon fractures can be caused by high-energy vertical forces which may result in long-term patient immobilization. Many experts in orthopedic surgery recommend the use of a Delta external fixator for type III Pilon fracture treatment. This device can promote immediate healing of fractured bone, minimizing the rate of complications as well as allowing early mobilization. The characteristics of different types of the Delta frame have not been demonstrated yet. By using the finite element method, this study was conducted to determine the biomechanical characteristics of six different configurations (Model 1 until Model 6)...
April 21, 2018: Medical & Biological Engineering & Computing
https://www.readbyqxmd.com/read/29183577/identification-and-characterisation-of-seventeen-glutathione-s-transferase-genes-from-the-cabbage-white-butterfly-pieris-rapae
#4
Su Liu, Yu-Xing Zhang, Wen-Long Wang, Bang-Xian Zhang, Shi-Guang Li
Insect glutathione S-transferases (GSTs) play essential roles in the detoxification of insecticides and other xenobiotic compounds. The cabbage white butterfly, Pieris rapae, is an economically important agricultural pest. In this study, 17 cDNA sequences encoding putative GSTs were identified in P. rapae. All cDNAs include a complete open reading frame and were designated PrGSTd1-PrGSTz2. Based on phylogenetic analysis, PrGSTs were divided into six classes (delta, epsilon, omega, sigma, theta and zeta). The exon-intron organizations of these PrGSTs were also analysed...
November 2017: Pesticide Biochemistry and Physiology
https://www.readbyqxmd.com/read/29044820/will-fluctuations-in-salt-marsh-mangrove-dominance-alter-vulnerability-of-a-subtropical-wetland-to-sea-level-rise
#5
Karen L McKee, William C Vervaeke
To avoid submergence during sea-level rise, coastal wetlands build soil surfaces vertically through accumulation of inorganic sediment and organic matter. At climatic boundaries where mangroves are expanding and replacing salt marsh, wetland capacity to respond to sea-level rise may change. To compare how well mangroves and salt marshes accommodate sea-level rise, we conducted a manipulative field experiment in a subtropical plant community in the subsiding Mississippi River Delta. Experimental plots were established in spatially equivalent positions along creek banks in monospecific stands of Spartina alterniflora (smooth cordgrass) or Avicennia germinans (black mangrove) and in mixed stands containing both species...
March 2018: Global Change Biology
https://www.readbyqxmd.com/read/29026767/socioeconomic-determinants-affecting-the-quality-of-life-among-diabetic-and-hypertensive-patients-in-a-rural-area-egypt
#6
Hewaida M ElShazly, Nagwa Nashat Hegazy
BACKGROUND: Diabetes mellitus and hypertension have a high prevalence of growth in all countries threatening to become a global epidemic risk, thus the quality of life this category of patients is extremely important. The aim of this study was to assess the socioeconomic determinants affecting the quality of life among diabetic and hypertensive patients. PATIENT AND METHODS: A descriptive cross-sectional study was conducted in a rural family health care center in El-Batanon village, in the delta region of Menoufia, Egypt...
January 2017: Journal of Family Medicine and Primary Care
https://www.readbyqxmd.com/read/28978580/use-of-optokinetics-based-okcsib-protocol-in-restoring-mobility-in-primary-progressive-ms
#7
Benjamin Chitambira, Ciara McConaghy
The case is of a mid-40s patient who developed progressive muscle weakness and loss of mobility over 6 weeks due to primary progressive multiple sclerosis. 2.5 days before admission, she became bed bound with weakness of 1/5 on the Oxford scale. She normally rested for a couple of days after similar periods of worsening symptoms and then got back on her feet although with worse balance. She was treated with optokinetic chart stimulation and sensory interaction for balance (OKCSIB) protocol. Within 2 days of intervention, she was mobile with a delta frame and supervision of one...
October 4, 2017: BMJ Case Reports
https://www.readbyqxmd.com/read/28812206/differences-in-femoral-neck-structure-between-elderly-caucasian-and-chinese-populations-a-cross-sectional-study-of-perth-beijing-cohorts
#8
L Wang, B C C Khoo, X G Cheng, K Brown, J R Lewis, Y B Su, Z Guo, K Li, R L Prince
Structural skeletal differences of the femoral neck of older Beijing-Chinese and Perth-Caucasian women were compared; adjusting for frame size-related differences, Beijing-Chinese have lower periosteal width; however, indices of internal bone distribution suggest that Beijing-Chinese may exhibit increased resistance to fracture that may relate to the reduced hip fracture incidence. INTRODUCTION: Ethnic differences in skeletal structure may relate to differences in hip fracture risk in Chinese and Caucasian populations...
August 15, 2017: Archives of Osteoporosis
https://www.readbyqxmd.com/read/28652318/a-framework-for-quantification-and-physical-modeling-of-cell-mixing-applied-to-oscillator-synchronization-in-vertebrate-somitogenesis
#9
Koichiro Uriu, Rajasekaran Bhavna, Andrew C Oates, Luis G Morelli
In development and disease, cells move as they exchange signals. One example is found in vertebrate development, during which the timing of segment formation is set by a 'segmentation clock', in which oscillating gene expression is synchronized across a population of cells by Delta-Notch signaling. Delta-Notch signaling requires local cell-cell contact, but in the zebrafish embryonic tailbud, oscillating cells move rapidly, exchanging neighbors. Previous theoretical studies proposed that this relative movement or cell mixing might alter signaling and thereby enhance synchronization...
August 15, 2017: Biology Open
https://www.readbyqxmd.com/read/28580339/can-evolutionary-based-brain-map-be-used-as-a-complementary-diagnostic-tool-with-fmri-ct-and-pet-for-schizophrenic-patients
#10
R Boostani, M Sabeti
OBJECTIVE: In this research, a new approach termed as "evolutionary-based brain map" is presented as a diagnostic tool to classify schizophrenic and control subjects by distinguishing their electroencephalogram (EEG) features. METHODS: Particle swarm optimization (PSO) is employed to find discriminative frequency bands from different EEG channels. By deploying the energy of those selected frequency bands from different channels within each time frame (window) on the scalp geometry, a sort of two dimensional points along with their values are created; by applying Lagrange interpolation, an image can be constructed...
June 2017: Journal of Biomedical Physics & Engineering
https://www.readbyqxmd.com/read/28574466/a-robust-96-6-db-sndr-50-khz-bandwidth-switched-capacitor-delta-sigma-modulator-for-ir-imagers-in-space-instrumentation
#11
Michele Dei, Stepan Sutula, Jose Cisneros, Ernesto Pun, Richard Jan Engel Jansen, Lluís Terés, Francisco Serra-Graells
Infrared imaging technology, used both to study deep-space bodies' radiation and environmental changes on Earth, experienced constant improvements in the last few years, pushing data converter designers to face new challenges in terms of speed, power consumption and robustness against extremely harsh operating conditions. This paper presents a 96.6-dB-SNDR (Signal-to-Noise-plus-Distortion Ratio) 50-kHz-bandwidth fourth-order single-bit switched-capacitor delta-sigma modulator for ADC operating at 1.8 V and consuming 7...
June 2, 2017: Sensors
https://www.readbyqxmd.com/read/28490916/the-cannabis-withdrawal-syndrome-current-insights
#12
REVIEW
Udo Bonnet, Ulrich W Preuss
The cannabis withdrawal syndrome (CWS) is a criterion of cannabis use disorders (CUDs) ( Diagnostic and Statistical Manual of Mental Disorders - Fifth Edition ) and cannabis dependence (International Classification of Diseases [ICD]-10). Several lines of evidence from animal and human studies indicate that cessation from long-term and regular cannabis use precipitates a specific withdrawal syndrome with mainly mood and behavioral symptoms of light to moderate intensity, which can usually be treated in an outpatient setting...
2017: Substance Abuse and Rehabilitation
https://www.readbyqxmd.com/read/27684742/production-of-materials-with-spatially-controlled-cross-link-density-via-vat-photopolymerization
#13
Gregory I Peterson, Johanna J Schwartz, Di Zhang, Benjamin M Weiss, Mark A Ganter, Duane W Storti, Andrew J Boydston
We describe an efficient method to produce objects comprising spatially controlled and graded cross-link densities using vat photopolymerization additive manufacturing (AM). Using a commercially available diacrylate-based photoresin, 3D printer, and digital light processing (DLP) projector, we projected grayscale images to print objects in which the varied light intensity was correlated to controlled cross-link densities and associated mechanical properties. Cylinder and bar test specimens were used to establish correlations between light intensities used for printing and cross-link density in the resulting specimens...
October 26, 2016: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/27648476/detection-and-mapping-of-cannabinoids-in-single-hair-samples-through-rapid-derivatization-and-matrix-assisted-laser-desorption-ionization-mass-spectrometry
#14
Emma Beasley, Simona Francese, Tom Bassindale
The sample preparation method reported in this work has permitted for the first time the application of matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) profiling and imaging for the detection and mapping of cannabinoids in a single hair sample. MALDI-MS imaging analysis of hair samples has recently been suggested as an alternative technique to traditional methods of GC/MS and LC/MS due to simpler sample preparation, the ability to detect a narrower time frame of drug use, and a reduction in sample amount required...
October 18, 2016: Analytical Chemistry
https://www.readbyqxmd.com/read/27597254/driving-under-the-effect-of-drugs-hair-analysis-in-order-to-evaluate-recidivism
#15
Giovanna Tassoni, Marta Cippitelli, Dora Mirtella, Rino Froldi, Giovanni Ottaviani, Massimiliano Zampi, Mariano Cingolani
Under Italian law drug addiction and regular drug abuse are incompatible with driving ability. One important problem with the enforcement of the impaired driving law is the large number of people that re-offend. To regain their license, offenders must be drug-free for the duration of an observation period, according to the judgement of a medical commission. The exclusion of illicit drug use is determined by toxicological analysis. A few studies exist that have used a hair matrix to monitor recidivism. Hair is an attractive matrix for monitoring drug recidivism, due to the large time window for drug detection, and to the non-alterability of this matrix...
October 2016: Forensic Science International
https://www.readbyqxmd.com/read/27511048/small-world-characteristics-of-cortical-connectivity-changes-in-acute-stroke
#16
Pietro Caliandro, Fabrizio Vecchio, Francesca Miraglia, Giuseppe Reale, Giacomo Della Marca, Giuseppe La Torre, Giordano Lacidogna, Chiara Iacovelli, Luca Padua, Placido Bramanti, Paolo Maria Rossini
Background After cerebral ischemia, disruption and subsequent reorganization of functional connections occur both locally and remote to the lesion. Recently, complexity of brain connectivity has been described using graph theory, a mathematical approach that depicts important properties of complex systems by quantifying topologies of network representations. Functional and dynamic changes of brain connectivity can be reliably analyzed via electroencephalography (EEG) recordings even when they are not yet reflected in structural changes of connections...
January 2017: Neurorehabilitation and Neural Repair
https://www.readbyqxmd.com/read/27416620/current-status-of-frameless-anchored-iud-for-immediate-intracesarean-insertion
#17
Dirk Wildemeersch, Norman D Goldstuck, Thomas Hasskamp
Immediate postpartum intrauterine device (IUD) insertion deserves great attention as it can provide immediate, timely and convenient contraception plus the added benefit of preventing repeat unintended pregnancies. Although women post vaginal delivery can benefit from immediate post-placenta contraception, women undergoing Cesarean section clearly need contraception, as an inter-delivery interval shorter than 18 months places them at a high risk for uterine rupture. The main drawback of currently available framed IUD devices for immediate postpartum insertion of an IUD is their high expulsion and displacement rates when inserted immediately postpartum after both vaginal and Cesarean delivery...
January 2016: Developmental Period Medicine
https://www.readbyqxmd.com/read/27108112/delta-like-1-homolog-in-capra-hircus-molecular-characteristics-expression-pattern-and-phylogeny
#18
Jiangtao Hu, Wei Zhao, Siyuan Zhan, Ping Xiao, Jingxuan Zhou, Linjie Wang, Li Li, Hongping Zhang, Lili Niu, Tao Zhong
To research the molecular characteristics, expression pattern and phylogeny of the Delta-like 1 homolog gene (Dlk1) in goats. Dlk1 transcripts were identified in the Jianyang Da'er goats by reverse-transcription polymerase chain reaction (RT-PCR). Phylogenetic trees were constructed by Bayesian inference and neighbor-joining methods. Quantitative real-time PCR (qPCR), western blotting and in situ hybridization were performed to analyze the expression pattern of Dlk1. Five alternatively transcripts were identified in different tissues and designated as Dlk1-AS1, 2, 3, 4 and 5...
June 2016: Molecular Biology Reports
https://www.readbyqxmd.com/read/26955362/brain-oscillations-in-sport-toward-eeg-biomarkers-of-performance
#19
REVIEW
Guy Cheron, Géraldine Petit, Julian Cheron, Axelle Leroy, Anita Cebolla, Carlos Cevallos, Mathieu Petieau, Thomas Hoellinger, David Zarka, Anne-Marie Clarinval, Bernard Dan
Brain dynamics is at the basis of top performance accomplishment in sports. The search for neural biomarkers of performance remains a challenge in movement science and sport psychology. The non-invasive nature of high-density electroencephalography (EEG) recording has made it a most promising avenue for providing quantitative feedback to practitioners and coaches. Here, we review the current relevance of the main types of EEG oscillations in order to trace a perspective for future practical applications of EEG and event-related potentials (ERP) in sport...
2016: Frontiers in Psychology
https://www.readbyqxmd.com/read/26660178/quantification-of-11-carboxy-delta-9-tetrahydrocannabinol-thc-cooh-in-meconium-using-gas-chromatography-mass-spectrometry-gc-ms
#20
Judy Peat, Brehon Davis, Clint Frazee, Uttam Garg
Maternal substance abuse is an ongoing concern and detecting drug use during pregnancy is an important component of neonatal care when drug abuse is suspected. Meconium is the preferred specimen for drug testing because it is easier to collect than neonatal urine and it provides a much broader time frame of drug exposure. We describe a method for quantifying 11-carboxy-delta-9-tetrahydrocannabinol (THC-COOH) in meconium. After adding a labeled internal standard (THC-COOH D9) and acetonitrile, samples are sonicated to release both free and conjugated THC-COOH...
2016: Methods in Molecular Biology
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