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https://www.readbyqxmd.com/read/29353443/role-of-fluorescent-in-situ-hybridization-cholangioscopic-biopsies-and-eus-fna-in-the-evaluation-of-biliary-strictures
#1
Christian Brooks, Valerie Gausman, Chanthel Kokoy-Mondragon, Khushboo Munot, Sunil P Amin, Amit Desai, Claudine Kipp, John Poneros, Amrita Sethi, Frank G Gress, Michel Kahaleh, Vundavalli V Murty, Reem Sharaiha, Tamas A Gonda
BACKGROUND AND AIMS: Our goal was to compare the diagnostic accuracy of FISH in the detection of malignancy compared with other standard diagnostic modalities, including brush cytology and biopsy specimens over a 10-year period of prospective data collection. METHODS: We conducted a review of all consecutive biliary strictures evaluated between 2006 and 2016. Patients with a final pathologic diagnosis or conclusive follow-up were included. We evaluated the performance of FISH polysomy (CEP 3, 7, and 17) and 9p21 deletion as well as cholangioscopic biopsy (CBx) and EUS-FNA...
January 20, 2018: Digestive Diseases and Sciences
https://www.readbyqxmd.com/read/29352234/bi-exponential-3d-t1%C3%AF-mapping-of-whole-brain-at-3%C3%A2-t
#2
Rajiv G Menon, Azadeh Sharafi, Johannes Windschuh, Ravinder R Regatte
Detection of multiple relaxation pools using MRI is useful in a number of neuro-pathologies including multiple sclerosis (MS), Alzheimer's, and stroke. In this study we evaluate the feasibility of using T1ρ imaging for the detection of bi-exponential decays in the human brain. A prospective T1ρ imaging study was performed on model relaxation phantoms (eggs) and 7 healthy volunteers. The data was fitted using a single pool and a 2-pool model to estimate mono- and bi-exponential T1ρ maps, respectively. Bi-exponential decays were identified in the gray matter (GM) and white matter (WM) of the brain with 40...
January 19, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29345461/a-combined-iron-hydroxytriazole-dual-catalytic-system-for-site-selective-oxidation-adjacent-to-azaheterocycles
#3
Julian C Cooper, Chaosheng Luo, Ryohei Kameyama, Jeffrey F Van Humbeck
This report details a new method for site-selective methylene oxidation adjacent to azaheterocycles. A dual catalysis approach, utilizing both an iron Lewis acid and an organic hydroxylamine catalyst proved highly effective. We demonstrate that this method provides complimentary selectivity to other know catalytic approaches and represents an improvement over current heterocycle-selective reactions that rely on stoichiometric activation.
January 18, 2018: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/29344008/understanding-the-factors-that-effect-maximal-fat-oxidation
#4
REVIEW
Troy Purdom, Len Kravitz, Karol Dokladny, Christine Mermier
Lipids as a fuel source for energy supply during submaximal exercise originate from subcutaneous adipose tissue derived fatty acids (FA), intramuscular triacylglycerides (IMTG), cholesterol and dietary fat. These sources of fat contribute to fatty acid oxidation (FAox) in various ways. The regulation and utilization of FAs in a maximal capacity occur primarily at exercise intensities between 45 and 65% VO2max, is known as maximal fat oxidation (MFO), and is measured in g/min. Fatty acid oxidation occurs during submaximal exercise intensities, but is also complimentary to carbohydrate oxidation (CHOox)...
2018: Journal of the International Society of Sports Nutrition
https://www.readbyqxmd.com/read/29342245/structure-function-relationships-of-olfactory-and-taste-receptors
#5
Maik Behrens, Loïc Briand, Claire A de March, Hiroaki Matsunami, Atsuko Yamashita, Wolfgang Meyerhof, Simone Weyand
The field of chemical senses has made major progress in understanding the cellular mechanisms of olfaction and taste in the past two decades. However, the molecular understanding of odour and taste recognition is still lagging far behind and will require solving multiple structures of the relevant full-length receptors in complex with native ligands to achieve this goal. However, the development of multiple complimentary strategies for the structure determination of G protein-coupled receptors (GPCRs) makes this goal realistic...
January 12, 2018: Chemical Senses
https://www.readbyqxmd.com/read/29341315/use-of-quantitative-real-time-pcr-to-determine-viability-of-streptococcus-equi-subspecies-equi-in-respiratory-secretions-from-horses-with-strangles
#6
N Pusterla, C M Leutenegger, S M Barnum, B A Byrne
BACKGROUND: In recent years, molecular approaches have been able to characterise the viability of equine upper respiratory tract pathogens using absolute molecular quantitation as well as detection of transcripts for virulence genes. OBJECTIVES: The objective this study was to investigate molecular surrogates for S. equi subspecies equi (S. equi) viability in biological samples from horses with strangles. STUDY DESIGN: Retrospective cross-sectional study...
January 17, 2018: Equine Veterinary Journal
https://www.readbyqxmd.com/read/29337176/comparison-of-the-diagnostic-performances-of-ultrasonography-ct-and-fine-needle-aspiration-cytology-for-the-prediction-of-lymph-node-metastasis-in-patients-with-lymph-node-dissection-of-papillary-thyroid-carcinoma-a-retrospective-cohort-study
#7
Na Lae Eun, Eun Ju Son, Jeong-Ah Kim, Hye Mi Gweon, Jung-Hyun Kang, Ji Hyun Youk
PURPOSE: To evaluate the diagnostic performances of ultrasonographic (US) findings, computed tomography (CT) findings and fine needle aspiration cytology (FNAC) for the prediction of cervical lymph node (LN) metastases of papillary thyroid carcinoma (PTC) to determine which LN should be dissected. METHODS: 376 LNs in 302 patients who underwent both US-guided skin surface LN markings and CT before LN dissection were analyzed retrospectively. Indications for LN dissection were suspicious US findings of LN metastases (n = 300), suspicious CT findings (n = 67) or surgeon's request (n = 9)...
January 11, 2018: International Journal of Surgery
https://www.readbyqxmd.com/read/29331931/resting-state-fmri-signals-in-offspring-of-parents-with-bipolar-disorder-at-the-high-risk-and-ultra-high-risk-stages-and-their-relations-with-cognitive-function
#8
Kangguang Lin, Robin Shao, Rui Lu, Kun Chen, Weicong Lu, Ting Li, Jiehua Kong, Kwok-Fai So, Guiyun Xu
BACKGROUND: Bipolar disorder (BD) has been associated with dysfunctional resting-state brain functioning. However, it is still not known whether the aberrant functioning occurs and predict cognitive functioning before illness onset. AIMS: We examined the resting-state regional and network dysfunctioning, and their correlates with neurocognitive performance, in the high-risk (HR) and ultra-high-risk (UHR) stages of bipolar disorder. METHODS: Using amplitude of low-frequency fluctuations (ALFF), region homogeneity (ReHo) and hypothesis-driven region-of-interest (ROI)-based connectivity, we examined resting-state fMRI data of 8- to 25-year-old healthy offspring (HR, n = 28) and offspring with subthreshold syndromes (UHR, n = 22) of a BD parent, and age-matched healthy controls without any personal or family psychopathology (HC, n = 46)...
January 8, 2018: Journal of Psychiatric Research
https://www.readbyqxmd.com/read/29329817/isothermal-chemical-denaturation-as-a-complimentary-tool-to-overcome-limitations-of-thermal-differential-scanning-fluorimetry-in-predicting-physical-stability-of-protein-formulations
#9
Hristo Svilenov, Uroš Markoja, Gerhard Winter
Various stability indicating techniques find application in the early stage development of novel therapeutic protein candidates. Some of these techniques are used to select formulation conditions that provide high protein physical stability. Such approach is highly dependent on the reliability of the stability indicating technique used. In this work, we present a formulation case study where we evaluate the ability of differential scanning fluorimetry (DSF) and isothermal chemical denaturation (ICD) to predict the physical stability of a model monoclonal antibody during accelerated stability studies...
January 9, 2018: European Journal of Pharmaceutics and Biopharmaceutics
https://www.readbyqxmd.com/read/29325698/what-causes-childhood-stunting-among-children-of-san-vicente-guatemala-employing-complimentary-system-analysis-approaches
#10
Lee E Voth-Gaeddert, Matthew Stoker, Devin Cornell, Daniel B Oerther
Guatemala has the sixth worst stunting rate with 48% of children under five years of age classified as stunted according to World Health Organization standards. This study utilizes two different yet complimentary system-analysis approaches to analyze correlations among environmental and demographic variables, environmental enteric dysfunction (EED), and child height-for-age (stunting metric) in Guatemala. Two descriptive models constructed around applicable environmental and demographic factors on child height-for-age and EED were analyzed using Network Analysis (NA) and Structural Equation Modeling (SEM)...
January 5, 2018: International Journal of Hygiene and Environmental Health
https://www.readbyqxmd.com/read/29316190/life-of-patients-10-years-after-a-successful-pediatric-intestinal-transplantation-in-europe
#11
Lorenzo Norsa, Girish Gupte, Esther Ramos Boluda, Francisca Joly, Olivier Corcos, Jacques Pirenne, Gustaf Herlenius, Florence Lacaille
A multicenter Europe-wide single point study in intestinal transplantation (ITx) centers was conducted to identify and describe patients surviving for more than 10 years after ITx in childhood. The health and nutritional status, care requirements and psychosocial status were recorded. Among 120 transplanted before 2005, 38 patients with a functioning graft were included. Thirty (79%) had an exclusive oral diet, 7 (18%) complimentary enteral nutrition for eating disorders, one a combination of parenteral and enteral nutrition...
January 9, 2018: American Journal of Transplantation
https://www.readbyqxmd.com/read/29316127/dual-ligand-enabled-non-directed-c-h-olefination-of-arenes
#12
Hao Chen, Philipp Wedi, Tim Meyer, Ghazal Tavakoli, Manuel van Gemmeren
The applicability of the Pd-catalyzed oxidative C-H olefination of arenes, also known as the Fujiwara-Moritani reaction, has traditionally been limited by the requirement for directing groups on the substrate or the need to use the arene in large excess, typically as (co)solvent. Herein we report the development of a catalytic system that, through the combined action of two complimentary ligands, enables the use of directing group free arenes as limiting reagents for the first time. The reactions proceed under a combination of both steric and electronic control and enable the application of this powerful reaction to valuable arenes, which cannot be utilized in excess...
January 9, 2018: Angewandte Chemie
https://www.readbyqxmd.com/read/29315226/unfolding-role-of-a-danger-molecule-adenosine-signaling-in-modulation-of-microbial-infection-and-host-cell-response
#13
REVIEW
Jaden S Lee, Özlem Yilmaz
Ectonucleotidases CD39 and CD73, specific nucleotide metabolizing enzymes located on the surface of the host, can convert a pro-inflammatory environment driven by a danger molecule extracellular-ATP to an adenosine-mediated anti-inflammatory milieu. Accordingly, CD39/CD73 signaling have has strongly implicated in modulating the intensity, duration, and composition of purinergic danger signals delivered to host. Recent studies have eluted potential roles for CD39 and CD73 in selective triggering of a variety of host immune cells and molecules in the presence of pathogenic microorganisms or microbial virulence molecules...
January 9, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29314642/venous-access-closure-from-a-to-z
#14
EDITORIAL
Ashok Seth, Ranjan Modi
Points to increasing need for achieving safe and secure closure of femoral venous access site after large caliber venous sheath for after structural interventions. Emphasizes the role of a simple, easy to learn "Z-stitch" for achieving femoral vein hemostasis leading to early ambulation. Cautions and advocates the use of multiple complimentary techniques for venous access closure after extreme large bore devices.
January 1, 2018: Catheterization and Cardiovascular Interventions
https://www.readbyqxmd.com/read/29306440/chemical-and-biophysical-approaches-for-complete-characterization-of-lectin-carbohydrate-interactions
#15
Sabrina Lusvarghi, Rodolfo Ghirlando, Jack R Davison, Carole A Bewley
Lectins are carbohydrate-binding proteins unrelated to antibodies or enzymes. While carbohydrates are present on all cells and pathogens, lectins are also ubiquitous in nature and their interactions with glycans mediate countless biological and physical interactions. Due to the multivalency found in both lectins and their glycan-binding partners, complete characterization of these interactions can be complex and typically requires the use of multiple complimentary techniques. In this chapter, we provide a general strategy and protocols for chemical and biophysical approaches that can be used to characterize carbohydrate-mediated interactions in the context of individual oligosaccharides, as part of a glycoprotein, and ending with visualization of interactions with whole virions...
2018: Methods in Enzymology
https://www.readbyqxmd.com/read/29302552/complementary-feeding-practices-among-children-aged-6-23-months-in-aligarh-uttar-pradesh
#16
Istiyaq Ahmad, Najam Khalique, Salman Khalil, Urfi, Mohd Maroof
Context: Complementary feeding practices play an important role in the growth and development of the children. Aims: The aim of this study is to determine the prevalence of complementary feeding practices among children aged 6-23 months and its association with various sociodemographic factors. Settings and Design: The study was a community-based, cross-sectional study conducted at field practice area of Urban Health Training Centre and Rural Health Training Centre, Department of Community Medicine, Jawaharlal Nehru Medical College, Aligarh Muslim University, Aligarh...
April 2017: Journal of Family Medicine and Primary Care
https://www.readbyqxmd.com/read/29300700/understanding-the-physiological-significance-of-four-inertial-gait-features-in-multiple-sclerosis
#17
Sriram Raju Dandu, Matthew M Engelhard, Asma Qureshi, Jiaqi Gong, John C Lach, Maite Brandt-Pearce, Myla D Goldman
Gait impairment in multiple sclerosis (MS) can result from muscle weakness, physical fatigue, lack of coordination, and other symptoms. Walking speed, as measured by a number of clinician-administered walking tests, is the primary measure of gait impairment used by clinical researchers, but inertial gait features from body-worn sensors have been proven to add clinical value. This paper seeks to understand and differentiate the physiological significance of four such features with proven value in MS to facilitate adoption by clinical researchers and incorporation in gait monitoring and analysis systems...
January 2018: IEEE Journal of Biomedical and Health Informatics
https://www.readbyqxmd.com/read/29296066/multimodality-imaging-assessment-of-a-caseous-calcification-of-the-mitral-valve-annulus
#18
Imen Hamdi, Chadia Chourabi, Younes Arous, Mehdi Ghommidh, Khalil Houissa, Abdeddayem Haggui, Nadhem Hajlaoui, Dhaker Lahidheb, Nejmeddine Ben Abdallah, Wafa Fehri, Habib Haouala
Caseous calcification of the mitral annulus (CCMA) is a rare echocardiographic finding. It is commonly misdiagnosed as an abscess, tumor or infective vegetation on the mitral valve. Since it is a benign process, differentiating it from malignant intra-cardiac mass is primordial to avoid unnecessary surgery. Various imaging modalities can be complimentary for definitive diagnosis. We present a case of CCMA in a 71-year-old female patient. Her medical history revealed hypertension, diabetes mellitus, hyperlipidaemia and coronary artery disease...
January 2018: Journal of the Saudi Heart Association
https://www.readbyqxmd.com/read/29289530/a-vascular-endothelial-growth-factor-dependent-sprouting-angiogenesis-assay-based-on-an-in-vitro-human-blood-vessel-model-for-the-study-of-anti-angiogenic-drugs
#19
Joris Pauty, Ryo Usuba, Irene Gayi Cheng, Louise Hespel, Haruko Takahashi, Keisuke Kato, Masayoshi Kobayashi, Hiroyuki Nakajima, Eujin Lee, Florian Yger, Fabrice Soncin, Yukiko T Matsunaga
Angiogenesis is the formation of new capillaries from pre-existing blood vessels and participates in proper vasculature development. In pathological conditions such as cancer, abnormal angiogenesis takes place. Angiogenesis is primarily carried out by endothelial cells, the innermost layer of blood vessels. The vascular endothelial growth factor-A (VEGF-A) and its receptor-2 (VEGFR-2) trigger most of the mechanisms activating and regulating angiogenesis, and have been the targets for the development of drugs...
December 20, 2017: EBioMedicine
https://www.readbyqxmd.com/read/29288920/diagnostic-potential-and-future-directions-of-biomarkers-in-gingival-crevicular-fluid-and-saliva-of-periodontal-diseases-review-of-the-current-evidence
#20
REVIEW
Noha Ayman Ghallab
OBJECTIVES: The holy grail of biomarker research in periodontology is to develop a high impact diagnostics which have a significant impact on clinical decision-making, patient outcomes and healthcare providers. In the field of periodontal diagnostics, oral fluid-based biomarkers have been studied mainly in the gingival crevicular fluid (GCF) and saliva. METHODS: A literature search was performed using the Cochrane library and PubMed databases from 2000 to January 2017...
December 23, 2017: Archives of Oral Biology
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