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https://www.readbyqxmd.com/read/27906107/previously-undiagnosed-hereditary-spherocytosis-in-a-patient-with-jaundice-and-pyelonephritis-a-case-report
#1
Yuki Tateno, Ryoji Suzuki, Yukihiro Kitamura
BACKGROUND: Hereditary spherocytosis is autosomal dominant inherited extravascular hemolytic disorder and is the commonest cause of inherited hemolysis in northern Europe and the United States. The classical clinical features of hereditary spherocytosis are anemia, jaundice, and splenomegaly. However, all of these classical features are not always revealed in the case of mild hemolysis or when hemolysis is well compensated. Patients with hereditary spherocytosis may remain undiagnosed for years if their hemolysis is mild...
December 1, 2016: Journal of Medical Case Reports
https://www.readbyqxmd.com/read/27904453/outcome-of-severe-infections-in-afebrile-neutropenic-cancer-patients
#2
Ksenija Strojnik, Ksenija Mahkovic-Hergouth, Barbara Jezersek Novakovic, Bostjan Seruga
BACKGROUND: In some neutropenic cancer patients fever may be absent despite microbiologically and/or clinically confirmed infection. We hypothesized that afebrile neutropenic cancer patients with severe infections have worse outcome as compared to cancer patients with febrile neutropenia. PATIENTS AND METHODS: We retrospectively analyzed all adult cancer patients with chemotherapy-induced neutropenia and severe infection, who were admitted to the Intensive Care Unit at our cancer center between 2000 and 2011...
December 1, 2016: Radiology and Oncology
https://www.readbyqxmd.com/read/27900293/combined-use-of-platelet-rich-plasma-micro-fat-in-sport-and-race-horses-with-degenerative-joint-disease-preliminary-clinical-study-in-eight-horses
#3
Fabrizio Bembo, Julia Eraud, Cecile Philandrianos, Baptiste Bertrand, Alain Silvestre, Julie Veran, Florence Sabatier, Guy Magalon, Jeremy Magalon
BACKGROUND: To assess the safety and potential efficacy of a standardized technique consisting of intra-articular injection of 10 cc of a homogeneous mixed product using autologous micro-fat and platelet rich plasma (PRP) (ratio 1:1) in the carpus or the fetlock joint of sport horses presenting degenerative joint disease (DJD). METHODS: Eight sport horses with DJD confirmed by radiography and ultrasonography and causing lameness and the impossibility to compete were treated...
April 2016: Muscles, Ligaments and Tendons Journal
https://www.readbyqxmd.com/read/27898361/movement-symptoms-in-european-moyamoya-angiopathy-first-systematic-questionnaire-study
#4
Markus Kraemer, Leonidas Trakolis, Jens Platzen, Jan Claudius Schwitalla, Anna Bersano, Philipp Albrecht, Marc Schlamann, Peter Berlit
OBJECTIVE: Movement disorders are a rare manifestation of Moyamoya angiopathy (MMA). Data on prevalence and clinical presentation are warranted. Possible involuntary movements include focal motor seizures, tremor, limb-shaking transient ischemic attacks, choreiform and spastic or dystonic movement disorders. PATIENTS AND METHODS: We developed a questionnaire to systematically assess movement disorders in MMA. Patients' history of involuntary movements and their clinical presentation were assessed systematically by interview...
November 23, 2016: Clinical Neurology and Neurosurgery
https://www.readbyqxmd.com/read/27897254/high-level-expression-of-improved-thermo-stable-alkaline-xylanase-variant-in-pichia-pastoris-through-codon-optimization-multiple-gene-insertion-and-high-density-fermentation
#5
Yihong Lu, Cheng Fang, Qinhong Wang, Yuling Zhou, Guimin Zhang, Yanhe Ma
In paper industry, xylanases are used to increase the pulp properties in bleaching process as its eco-friendly nature. The xylanases activity is hindered by high temperature and alkaline conditions with high enzyme production cost in the paper industry. Here, XynHB, an alkaline stable xylanase from Bacillus pumilus HBP8 was mutated at N188A to XynHBN188A. Expressed mutant in E. coli showed 1.5-fold higher xylanase activity than XynHB at 60 °C. The mutant expressed in Pichia pastoris was glycosylated, remained stable for 30 min at 60 °C...
November 29, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27892654/injectable-polysaccharide-hydrogels-as-biocompatible-platforms-for-localized-and-sustained-delivery-of-antibiotics-for-preventing-local-infections
#6
Ziyi Li, Chaoliang He, Baoming Yuan, Xiaoming Dong, Xuesi Chen
Biocompatible and antibacterial hydrogels have received increasing attention for preventing local bacterial infections. In this study, a type of polysaccharide hydrogels is prepared via the Schiff-based reaction at physiological conditions. The gelation time and mechanical property of the hydrogels are found to be dependent on the polysaccharide concentration and the polysaccharide weight ratio. 3-(4,5-Dimethyl-thiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay and live/dead assay indicate that the hydrogels display nontoxicity in vitro...
November 28, 2016: Macromolecular Bioscience
https://www.readbyqxmd.com/read/27887723/synergistic-effect-of-cavitation-and-agitation-on-protein-aggregation
#7
Tetsuo Torisu, Takahiro Maruno, Yoshinori Hamaji, Tadayasu Ohkubo, Susumu Uchiyama
It was recently reported that dropping induces protein aggregation due to the occurrence of cavitation. Agitation also causes protein aggregation. In this study, vials filled with antibody solution were subjected to a cycle of dropping and shaking using the friability testing apparatus to examine the combined effect of cavitation and agitation on protein aggregation. A cycle of dropping and shaking generated a massive amount of subvisible particles. Comparison of aggregation rate at different fill volumes indicated that shaking plays an important role in protein aggregation due to combination stress...
November 22, 2016: Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/27886480/floquet-realization-and-signatures-of-one-dimensional-anyons-in-an-optical-lattice
#8
Christoph Sträter, Shashi C L Srivastava, André Eckardt
We propose a simple scheme for mimicking the physics of one-dimensional anyons in an optical-lattice experiment. It relies on a bosonic representation of the anyonic Hubbard model to be realized via lattice-shaking-induced resonant tunneling against potential offsets, which are created by a combination of a lattice tilt and strong on-site interactions. No lasers additional to those used for the creation of the optical lattice are required. We also discuss experimental signatures of the continuous interpolation between bosons and fermions when the statistical angle θ is varied from 0 to π...
November 11, 2016: Physical Review Letters
https://www.readbyqxmd.com/read/27884954/impact-of-external-pneumatic-compression-target-inflation-pressure-on-transcriptome-wide-rna-expression-in-skeletal-muscle
#9
Jeffrey S Martin, Wesley C Kephart, Cody T Haun, Anna E McCloskey, Joshua J Shake, Christopher B Mobley, Michael D Goodlett, Andreas Kavazis, David D Pascoe, Lee Zhang, Michael D Roberts
Next-generation RNA sequencing was employed to determine the acute and subchronic impact of peristaltic pulse external pneumatic compression (PEPC) of different target inflation pressures on global gene expression in human vastus lateralis skeletal muscle biopsy samples. Eighteen (N = 18) male participants were randomly assigned to one of the three groups: (1) sham (n = 6), 2) EPC at 30-40 mmHg (LP-EPC; n = 6), and 3) EPC at 70-80 mmHg (MP-EPC; n = 6). One hour treatment with sham/EPC occurred for seven consecutive days...
November 2016: Physiological Reports
https://www.readbyqxmd.com/read/27882738/schistosoma-haematobium-infection-is-associated-with-increased-urine-foam
#10
Johnathan M Sheele, Sarah Baddorf, Jimmy H Kihara
We compared urine microscopy and dipstick results for urine foam from 59 children in a Schistosoma haematobium-endemic area in a blinded manner. The sensitivity and specificity, respectively, for diagnosing S. haematobium compared with microscopy was: 74% and 72% for the shake test; 61% and 97% for microscopic hematuria; and 43% and 83% for proteinuria. When >17 eggs/10 mL urine was detected on microscopy, the sensitivity and specificity, respectively, were: 100% and 72% for the shake test; 90% and 97% for microscopic hematuria; and 80% and 83% for proteinuria...
November 2016: Pediatrics International: Official Journal of the Japan Pediatric Society
https://www.readbyqxmd.com/read/27881735/therapy-activated-stromal-cells-can-dictate-tumor-fate
#11
Robert S Kerbel, Yuval Shaked
In this issue of JEM, Chan et al. describe a novel way by which an investigational form of chemotherapy known as low-dose metronomic chemotherapy can inhibit tumor growth, which also has therapeutic implications for targeting tumor-initiating cells (TICs), the tumor stroma, and chemokine receptors, as well as invasion and metastasis.
November 23, 2016: Journal of Experimental Medicine
https://www.readbyqxmd.com/read/27878590/use-of-saline-waste-water-from-demineralization-of-cheese-whey-for-cultivation-of-schizochytrium-limacinum-pa-968-and-japonochytrium-marinum-an-4
#12
Tomas Humhal, Petr Kastanek, Zuzana Jezkova, Anna Cadkova, Jana Kohoutkova, Tomas Branyik
Saline waste water from demineralization of cheese whey was used as the main component of waste saline medium (WSM) for cultivation of thraustochytrids. The suitability of WSM for cultivation of Schizochytrium limacinum PA-968 and Japonochytrium marinum AN-4 was evaluated by comparison with cultivation on nutrient medium (NM) in shake flask and fermenter cultures. Biomass productivities achieved in WSM for the thraustochytrids were comparable with those in NM for both shake flask and fermenter cultures. The maximum total lipid content (56...
November 23, 2016: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/27876710/enhancement-of-1-3-dihydroxyacetone-production-from-gluconobacter-oxydans-by-combined-mutagenesis
#13
Xi Lin, Sha Liu, Guangrong Xie, Jing Chen, Penghua Li, Jianhua Chen
Wild strain L-6 was subjected to combined mutagenesis, including UV irradiation, atmospheric and room temperature plasma, and ion beam implantation, to increase the yield of 1,3-dihydroxyacetone (DHA). With application of a high-throughput screening method, mutant Gluconobacter oxydans I-2-239 with a DHA productivity of 103.5 g/l in flask-shake fermentation was finally obtained with the starting glycerol concentration of 120 g/l, which was 115.7% higher than the wild strain. The cultivation time also decreased from 54 h to 36 h...
November 28, 2016: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27875082/effects-of-trazodone-on-behavioral-signs-of-stress-in-hospitalized-dogs
#14
Shana E Gilbert-Gregory, Jason W Stull, Mary Rose Rice, Meghan E Herron
OBJECTIVE To determine the effects of trazodone treatment on behavioral signs of stress in hospitalized dogs. DESIGN Prospective observational study. ANIMALS 120 client-owned dogs. PROCEDURES Hospitalized dogs administered trazodone (n = 60) were observed for stress-related signs or behaviors ≤ 45 minutes after the drug was administered (time 1) and approximately 90 minutes later (time 2). Dogs that did not receive trazodone (n = 60) were selected to serve as controls for environmental stimuli that could affect behavior and were observed at the same times...
December 1, 2016: Journal of the American Veterinary Medical Association
https://www.readbyqxmd.com/read/27871453/chasing-dizzy-chimera-diagnosis-of-combined-peripheral-and-central-vestibulopathy
#15
Seo-Young Choi, Hyo-Jung Kim, Ji-Soo Kim
Diagnosis of combined peripheral and central vestibulopathy remains a challenge since the findings from peripheral vestibular involvements may overshadow those from central vestibular disorders or vice versa. The aim of this study was to enhance detection of these intriguing disorders by characterizing the clinical features and underlying etiologies. We had recruited 55 patients with combined peripheral and central vestibulopathy at the Dizziness Clinic of Seoul National University Bundang Hospital from 2003 to 2013...
December 15, 2016: Journal of the Neurological Sciences
https://www.readbyqxmd.com/read/27866481/effect-of-inoculum-age-and-physical-parameters-on-in-vitro-culture-of-the-entomopathogenic-nematode-steinernema-feltiae
#16
L G Leite, D I Shapiro-Ilan, S Hazir, M A Jackson
Entomopathogenic nematodes (EPNs) of the families Steinernematidae and Heterorhabditidae have a symbiotic association with bacteria which makes them virulent against insects. EPNs have been mass produced using in vivo and in vitro methods, including both solid and liquid fermentation. This study assessed the effect of nematode inoculum age on the production of Steinernema feltiae in liquid, solid and biphasic processes. Several physical parameters were also assessed: the effect of medium viscosity, flask size and aeration speed on the recovery and yield of infective juveniles (IJs)...
November 21, 2016: Journal of Helminthology
https://www.readbyqxmd.com/read/27864159/high-level-expression-and-efficient-refolding-of-therapeutically-important-recombinant-human-interleukin-3-hil-3-in-e-%C3%A2-coli
#17
Vikas Kumar Dagar, Adivitiya, Yogender Pal Khasa
Human interleukin-3 (hIL-3) is a pleiotropic cytokine that stimulates the differentiation and proliferation of multipotent hematopoietic cells thus making it a therapeutically important molecule. In this study, its poor expression yield was improved by addressing various upstream bottlenecks in E. coli heterologous system. The codon-optimized hIL-3 gene was cloned under various signal sequences and solubility enhancer fusion tags for its hyper-expression under a strong T7 promoter. The optimization of shake flask expression studies resulted in a hIL-3 protein concentration of 225 mg/L in the form of inclusion bodies (IBs)...
November 15, 2016: Protein Expression and Purification
https://www.readbyqxmd.com/read/27864033/bronchiectasis-shaking-off-its-orphan-status
#18
Paul J Torzillo
No abstract text is available yet for this article.
November 15, 2016: Lancet Respiratory Medicine
https://www.readbyqxmd.com/read/27859662/a-powerful-cd8-t-cell-stimulating-d-tetra-peptide-hydrogel-as-a-very-promising-vaccine-adjuvant
#19
Zichao Luo, Qinjie Wu, Chengbiao Yang, Huaimin Wang, Tao He, Youzhi Wang, Zhongyan Wang, Hao Chen, Xingyi Li, Changyang Gong, Zhimou Yang
A novel vaccine adjuvant based on a supramolecular hydrogel of a D-tetra-peptide is reported. Antigens can be easily incorporated into the hydrogel by a vortex or by gently shaking before injection. The vaccines can stimulate strong CD8+ T-cell responses, which significantly inhibits tumor growth. This novel adjuvant is expected to enable a wide range of sub-unit vaccines and help the production of antibodies.
November 14, 2016: Advanced Materials
https://www.readbyqxmd.com/read/27856860/shaking-up-the-tree-of-life
#20
Elizabeth Pennisi
No abstract text is available yet for this article.
November 18, 2016: Science
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