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Dong Wang, Huifang Su, Ryan T K Kwok, Xianglong Hu, Hang Zou, Qianxin Luo, Michelle M S Lee, Wenhan Xu, Jacky W Y Lam, Ben Zhong Tang
The synthesis of water-soluble near-infrared (NIR)-emissive fluorescent molecules with aggregation-induced emission (AIE) characteristics and theranostic functions is highly desirable but remains challenging. In this work, we designed and readily prepared for the first time such a molecule with AIE features, good water-solubility and intense emission in the NIR region. This AIE luminogen (AIEgen) is able to specifically "light up" the cell membrane without the involvement of a washing procedure. Interestingly, the staining process can be performed by simply shaking the culture with cells at room temperature for only a few seconds after the addition of the AIEgen, indicating an ultrafast and easy-to-operate staining protocol...
April 21, 2018: Chemical Science
Julia Klopp, Aurélie Winterhalter, Rémy Gébleux, Daniela Scherer-Becker, Christian Ostermeier, Alvar D Gossert
We present protocols for high-level expression of isotope-labelled proteins in E. coli in cost-effective ways. This includes production of large amounts of unlabeled proteins and 13 C-methyl methionine labeling in rich media, where yields of up to a gram of soluble protein per liter of culture are reached. Procedures for uniform isotope labeling of 2 H, 13 C and 15 N using auto-induction or isopropyl-β-D-1-thiogalactopyranoside-induction are described, with primary focus on minimal isotope consumption and high reproducibility of protein expression...
May 19, 2018: Journal of Biomolecular NMR
Jiajia Zhang, Guoxiu Zhang, Wei Wang, Wei Wang, Dongzhi Wei
BACKGROUND: Cellulase can convert lignocellulosic feedstocks into fermentable sugars, which can be used for the industrial production of biofuels and chemicals. The high cost of cellulase production remains a challenge for lignocellulose breakdown. Trichoderma reesei RUT C30 serves as a well-known industrial workhorse for cellulase production. Therefore, the enhancement of cellulase production by T. reesei RUT C30 is of great importance. RESULTS: Two sets of novel minimal transcriptional activators (DBDace2 -VP16 and DBDcre1 -VP16) were designed and expressed in T...
May 17, 2018: Microbial Cell Factories
Jessica Rae Parker, Brain Bondy, Boris I Prilutsky, Gennady S Cymbalyuk
The ability of the same neuronal circuit to control different motor functions is an actively debated concept. Previously, we have shown in a model that a single multistable CPG could produce two different rhythmic motor patterns, slow and fast, corresponding to cat locomotion and paw-shaking. A locomotor-like rhythm (~2 Hz) and a paw-shake-like rhythm (~10 Hz) did coexist and, by applying a single pulse of current, we could switch the CPG from one regime to another (Bondy et al. 2016). Here, we investigate roles of slow intrinsic ionic currents in this multistability...
May 16, 2018: Journal of Neurophysiology
Nidhi Puranik, N K Tripathi, V Pal, Ajay Kumar Goel
Surface array protein (Sap) can be an important biomarker for specific detection of Bacillus anthracis , which is released by the bacterium during its growth in culture broth. In the present work, we have cloned and expressed Sap in Escherichia coli . The culture conditions and cultivation media were optimized and used in batch fermentation process for scale up of Sap in soluble form. The recombinant Sap was purified employing affinity chromatography followed by diafiltration. The final yield of purified protein was 20 and 46 mg/l of culture during shake flasks and batch fermentation, respectively...
May 2018: 3 Biotech
Nadeem Toodayan
No abstract text is available yet for this article.
May 21, 2018: Medical Journal of Australia
Andrea Accogli, Myriam Srour
No abstract text is available yet for this article.
May 15, 2018: Neurology
Joaquín González, José P Prieto, Paola Rodríguez, Matías Cavelli, Luciana Benedetto, Alejandra Mondino, Mariana Pazos, Gustavo Seoane, Ignacio Carrera, Cecilia Scorza, Pablo Torterolo
Ibogaine is a potent psychedelic alkaloid that has been the focus of intense research because of its intriguing anti-addictive properties. According to anecdotic reports, ibogaine has been originally classified as an oneirogenic psychedelic; i.e., induces a dream-like cognitive activity while awake. However, the effects of ibogaine administration on wakefulness (W) and sleep have not been thoroughly assessed. The main aim of our study was to characterize the acute effects of ibogaine administration on W and sleep...
2018: Frontiers in Pharmacology
Nicolai Kallscheuer, Jan Marienhagen
BACKGROUND: Hydroxybenzoic acids are industrially relevant aromatic compounds, which also play key roles in the microbial carbon metabolism, e.g., as precursors for the synthesis of cofactors or metal-chelating molecules. Due to its pronounced resistance to aromatics Corynebacterium glutamicum represents an interesting platform for production of these compounds. Unfortunately, a complex catabolic network for aromatic molecules prevents application of C. glutamicum for microbial production of aromatic compounds other than aromatic amino acids, which cannot be metabolized by this microorganism...
May 12, 2018: Microbial Cell Factories
A M El-Shamy, Ibrahim Abdelfattah, Ola I Elshafey, M F Shehata
A potential and cost-effective treatment method utilizing thermally activated bentonite was evaluated for the treatment of highly loaded real petroleum processing wastewater (COD = 4500 mg/L) in order to reduce its COD and improve the corrosion properties. A save discharging COD limit of the treated effluent (800 mg/L) is achieved by using 6 g/L of calcinated bentonite after reaching the steady state (1 h of shaking) at pH 5. The durability of bentonite is proved. The corrosion behavior of the treated wastewater was investigated for mild steel by using electrochemical and weight loss measurements...
May 9, 2018: Journal of Environmental Management
Hamid Shirkhanloo, Sara Davari Ahranjani
A simple and applied method based on ultrasound assisted-Ionic liquid trap-micro solid phase extraction (USA-ILT-μSPE) was demonstrated for preconcentration/separation of lead (Pb) in human blood and urine samples. By proposed method, the amine-functionalized bimodal mesoporous silica nanoparticles (NH2 -UVM7 ) as solid phase sorbent was used for lead analysis in human biological samples at pH ≈ 7.0. After shaking/centrifuging of sample, the Pb (II) was extracted with NH2 -UVM7 without any chelating agents, trapped in ionic liquid phase and settled down in conical centrifuge tube as a residual solution [Pb→:NH2 -UVM7 ]...
May 4, 2018: Journal of Pharmaceutical and Biomedical Analysis
Shengjun Wang, Yaoguang Wang, Peng George Wang, Min Chen, Yun Kong
The Ala-Pro-rich Antigen (Apa) from Mycobacterium tuberculosis is a mannosylated protein with immunogenic and antigenic properties. The O-mannosylation is essential for its biological function in the process of colonization and invasion of host cells by M. tuberculosis. In this work, the gene encoding Apa was cloned from M. tuberculosis and expressed in Pichia pastoris GS115. In shake-flasks, the recombinant Apa was secreted into the culture media and purified with a yield of 0.6 g/L. Both N- and O-glycans were found in recombinant Apa...
May 9, 2018: Protein Expression and Purification
Sarah E Minson, Men-Andrin Meier, Annemarie S Baltay, Thomas C Hanks, Elizabeth S Cochran
The basic physics of earthquakes is such that strong ground motion cannot be expected from an earthquake unless the earthquake itself is very close or has grown to be very large. We use simple seismological relationships to calculate the minimum time that must elapse before such ground motion can be expected at a distance from the earthquake, assuming that the earthquake magnitude is not predictable. Earthquake early warning (EEW) systems are in operation or development for many regions around the world, with the goal of providing enough warning of incoming ground shaking to allow people and automated systems to take protective actions to mitigate losses...
March 2018: Science Advances
Alexander Eck, Matthias Schmidt, Stefanie Hamer, Anna Joelle Ruff, Jan Förster, Ulrich Schwaneberg, Lars M Blank, Wolfgang Wiechert, Marco Oldiges
Background: Expanding the application of technical enzymes, e.g., in industry and agriculture, commands the acceleration and cost-reduction of bioprocess development. Microplates and shake flasks are massively employed during screenings and early phases of bioprocess development, although major drawbacks such as low oxygen transfer rates are well documented. In recent years, miniaturization and parallelization of stirred and shaken bioreactor concepts have led to the development of novel microbioreactor concepts...
2018: Fungal Biology and Biotechnology
Qian Zhang, Chongxian Liu, Guannan Ju, Mengjiao Cheng, Feng Shi
Macroscopic supramolecular assembly (MSA) is a recent advance in supramolecular chemistry that involves associating large building blocks with a size larger than 10 µm through noncovalent interactions. However, until now the applicable material system is rather limited to hydrogels, and MSA of rigid materials with supramolecular interactions widely used in molecular assembly has rarely been reported due to the difficulty in achieving multivalency between rigid surfaces. Herein, the concept of flexible spacing coating is applied with highly flowable properties, and the electrostatic-interaction-driven MSA of relatively rigid polydimethylsiloxane building blocks is demonstrated...
May 11, 2018: Macromolecular Rapid Communications
Aleš Gregorc, Mohamed Alburaki, Blair Sampson, Patricia R Knight, John Adamczyk
The efficacies of various acaricides in order to control a parasitic mite, the Varroa mite, Varroa destructor , of honey bees, were measured in two different settings, namely, in laboratory caged honey bees and in queen-right honey bee colonies. The Varroa infestation levels before, during, and after the acaricide treatments were determined in two ways, namely: (1) using the sugar shake protocol to count mites on bees and (2) directly counting the dead mites on the hive bottom inserts. The acaricides that were evaluated were coumaphos, tau-fluvalinate, amitraz, thymol, and natural plant compounds (hop acids), which were the active ingredients...
May 10, 2018: Insects
Sa Wang, Shu Hai Guo, Jia Ning Wang, Feng Mei Li, Bo Wu, Qiang Zhang
It is of great practical significance to rapidly screen the mixed microbial flora for degradation of complex organic pollutants. It was the first time to rapidly calibrate the petroleum hydrocarbon degradation ability of highly efficient degrading microflora by measuring enzyme activity analyzed by the MicroRespTM technology. The results were validated by the traditional shaking culture. According to the activity analysis of exoenzymes, endoenzymes and mixed enzymes, the degradation abilities of microflora and enzymes (including exoenzymes, endoenzymes and mixed enzymes) on petroleum hydrocarbon was investigated...
June 18, 2017: Ying Yong Sheng Tai Xue Bao, the Journal of Applied Ecology
Bin Zhang, Bang-Ce Ye
5-Aminolevulinic acid (ALA) is a non-protein amino acid with a significant potential for cancer treatment and plant stress resistance. Microbial fermentation has gradually replaced the traditional chemical-based method for ALA production, thus increasing the need for high-ALA-producing strains. In this study, we engineered the glutamate producing strain, Corynebacterium glutamicum S9114, for ALA production. To efficiently convert l-glutamate to ALA, hemA and hemL from Salmonella typhimurium and Escherichia coli were tandemly overexpressed...
May 2018: 3 Biotech
Takahiro Sasaki, Shoichi Tahara, Marie Tanaka, Toshiko Mizumachi, Yukiko Yamajima, Hiroyuki Miyakawa, Yoko Uematsu, Kimio Monma
A simple and useful method for the determination of nitrite in meat and fish products was developed. The sample (2.5 g) was extracted and cleaned up by dialysis in tris hydroxymethyl aminomethane solution with shaking at 80℃ for 2.5 hr. Nitrite in the dialysate was quantified by colorimetric analysis. Furthermore, the dialysate was cleaned up with SPE under cooling, and nitrite in the resulting solution was determined using LC-UV with an anion exchange column for confirmation. The recoveries of nitrite from frankfurter and fish sausage, fortified at the levels of 0...
2018: Shokuhin Eiseigaku Zasshi. Journal of the Food Hygienic Society of Japan
Kazuho Inaba, Tomoyoshi Murata, Shigeki Yamamura, Masaaki Nagano, Kazuhiro Iwasaki, Daisuke Nakajima, Hidetaka Takigami
The contents and elution behavior of metals in consumer electronics parts were determined so as to understand their maximum environmental risk. Elements contained most in printed-circuit boards were Cu, Si, Br, Ca, Al, Sn, Pb, Sb, Ba, Fe, Ni, Ti, and Zn; in cathode-ray tube glass were Si, Pb, Ba, Sr, Zn, Zr, Ca, and Sb; in arsenic contained liquid-crystal displays were Si, Ca, Sr, Ba, As, and Fe; and in antimony contained liquid-crystal displays were Si, Ba, Ca, Sb, Sr, Fe, and Sn. The elements eluted most from printed-circuit boards were Zn, Pb, and Cu; from cathode-ray tube glass were Pb, Zn, B, Ba, and Si; and from liquid-crystal displays were B and Si, and the toxic As and Sb...
2018: Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry
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