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https://www.readbyqxmd.com/read/28937864/determination-of-seven-pyrethroid-pesticides-residue-in-vegetables-by-gas-chromatography-using-carboxylated-multi-walled-carbon-nanotubes-as-dispersion-solid-phase-extraction-sorbent
#1
Peng Sun, Yuling Gao, Chong Xu, Yongfu Lian
:A novel carboxylated multi-walled carbon nanotubes (MWCNTs-COOH) dispersive solid phase extraction (d-SPE) method combined with gas chromatography (GC) with ECD detection was developed for the determination of seven pyrethroid pesticides in cucumber, spinach, eggplant, tomato and carrot. We optimized d-SPE conditions including the type and volume of the extractant, the type and amount of the sorbent, and shaking time. Under the optimal conditions, the linear range was from 2.0 to 2000 μg kg(-1). The recoveries were from 88...
September 22, 2017: Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
https://www.readbyqxmd.com/read/28936724/classics-in-abdominal-imaging-the-putty-kidney
#2
REVIEW
Dana C Galván, Shaked Laks
No abstract text is available yet for this article.
September 21, 2017: Abdominal Radiology
https://www.readbyqxmd.com/read/28936604/production-partial-optimization-and-characterization-of-keratinase-enzyme-by-arthrobacter-sp-nfh5-isolated-from-soil-samples
#3
Nirmal Chandra Barman, Fatema Tuj Zohora, Keshob Chandra Das, Md Golam Mowla, Nilufa Akhter Banu, Md Salimullah, Abu Hashem
The study was conducted to select the best promising keratinolytic bacterial strain. A good keratinase positive bacterium isolated from the soil samples of Hazaribagh tannery industrial zone, Dhaka was identified as Arthrobacter genus depending on the conventional techniques and confirmed as Arthrobacter sp. by sequencing 16S rRNA gene. The medium components and culture conditions were optimized to enhance keratinase production through shake flask culture. Keratin and feather powder (10 g/l or 1%) were good substrates for the highest keratinase production along with yeast extract (0...
September 21, 2017: AMB Express
https://www.readbyqxmd.com/read/28934999/vortex-assisted-modified-dispersive-liquid-liquid-microextraction-of-trace-levels-of-cadmium-in-surface-water-and-groundwater-samples-of-tharparkar-pakistan-optimized-by-multivariate-technique
#4
Sooraj Nizamani, Tasneem G Kazi, Hassan I Afridi, Sehrish Talpur, Ayaz Lashari, Anjum Lashari, Jamshed Ali
A simple vortex-assisted modified dispersive liquid–liquid microextraction procedure is proposed forthe enrichment of cadmium (Cd(+2)) in surface (stored rainwater) and groundwater of the Tharparkar district in Pakistan, before analysis by flame atomic absorption spectrometry. Ammonium pyrrolidinedithiocarbamate was used as a ligand to make a hydrophobic complex of Cd(+2), which was extracted in an ionic liquid (1-butyl-3-methylimidazolium hexafluorophosphate), and the nonionic surfactant Triton X-114 was applied as a dispersing medium...
September 21, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28934944/knowledge-attitude-and-practices-on-intestinal-schistosomiasis-among-primary-schoolchildren-in-the-lake-victoria-basin-rorya-district-north-western-tanzania
#5
David Z Munisi, Joram Buza, Emmanuel A Mpolya, Teckla Angelo, Safari M Kinung'hi
BACKGROUND: Globally school-age children, adolescents and young adults bear the highest burden of schistosomiasis. When developing a specific intervention to improve community's knowledge, attitudes, and practices (KAPs), existing KAPs must be taken into account. Therefore, this study was designed to determine schoolchildren's KAPs on schistosomiasis in the study area. METHODS: A cross-sectional study was conducted in Busanga and Kibuyi villages involving 513 schoolchildren...
September 21, 2017: BMC Public Health
https://www.readbyqxmd.com/read/28934479/targeted-reconstruction-of-t-cell-receptor-sequence-from-single-cell-rna-seq-links-cdr3-length-to-t-cell-differentiation-state
#6
Shaked Afik, Kathleen B Yates, Kevin Bi, Samuel Darko, Jernej Godec, Ulrike Gerdemann, Leo Swadling, Daniel C Douek, Paul Klenerman, Eleanor J Barnes, Arlene H Sharpe, W Nicholas Haining, Nir Yosef
The T cell compartment must contain diversity in both T cell receptor (TCR) repertoire and cell state to provide effective immunity against pathogens. However, it remains unclear how differences in the TCR contribute to heterogeneity in T cell state. Single cell RNA-sequencing (scRNA-seq) can allow simultaneous measurement of TCR sequence and global transcriptional profile from single cells. However, current methods for TCR inference from scRNA-seq are limited in their sensitivity and require long sequencing reads, thus increasing the cost and decreasing the number of cells that can be feasibly analyzed...
September 19, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28934417/production-of-3-geranyl-4-hydroxybenzoate-acid-in-yeast-an-important-intermediate-of-shikonin-biosynthesis-pathway
#7
Sheng Wang, Ruishan Wang, Tan Liu, Zhilai Zhan, Liping Kang, Yanan Wang, Chaogeng Lv, Daniele Werck-Reichhart, Lanping Guo, Luqi Huang
Shikonin and its derivatives are the main active components in the medicinal plant Arnebia euchroma and possess extensive pharmaceutical properties. In this study, we developed an optimized yeast system to obtain high-level production of 3-geranyl-4-hydroxybenzoate acid (GBA), an important intermediate involved in shikonin biosynthesis pathway. For host selection, recombinant expression of p-hydroxybenzoate:geranyltransferase (PGT) derived from A. euchroma was performed in Saccharomyces cerevisiae WAT11U strain and high yield monoterpene strain...
November 1, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28934012/liquid-chromatography-tandem-mass-spectrometry-method-for-simultaneous-quantification-of-azoxystrobin-and-its-metabolites-azoxystrobin-free-acid-and-2-hydroxybenzonitrile-in-greenhouse-grown-lettuce
#8
Maheswor Gautam, Inge S Fomsgaard
Lettuce is an important part of the diet in Europe. The permitted levels of pesticides in lettuce are strictly regulated and there is growing urge among food safety authorities to analyse pesticide metabolites as well. Azoxystrobin is one of pesticides that is frequently detected in lettuce. Although there are several analytical methods for the determination of azoxystrobin in lettuce, a sensitive method for the determination of its metabolites in lettuce is lacking. This study aimed at developing an extraction and LC-MS/MS method for the simultaneous determination of azoxystrobin, and its metabolites azoxystrobin free acid and 2-hydroxybenzonitrile in lettuce...
September 21, 2017: Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
https://www.readbyqxmd.com/read/28929429/segregation-of-a-binary-granular-mixture-in-a-vibrating-sawtooth-base-container
#9
Shahin Mobarakabadi, Neda Adrang, Mehdi Habibi, Ehsan Nedaaee Oskoee
A granular mixture of identical particles of different densities can be segregated when the system is shaken. We present an efficient method of continuously segregating a flow of randomly mixed identical spherical particles of different densities by shaking them in a quasi-two-dimensional container with a sawtooth-shaped base. Using numerical simulation we study the effect of direction of shaking (horizontal/vertical), geometry of the sawtooth, and the friction coefficient between the grains and the container walls on the segregation quality...
September 2017: European Physical Journal. E, Soft Matter
https://www.readbyqxmd.com/read/28928028/two-stage-heterotrophy-photoinduction-culture-of-scenedesmus-incrassatulus-potential-for-lutein-production
#10
Liliana Flórez-Miranda, Rosa Olivia Cañizares-Villanueva, Orlando Melchy-Antonio, Fernando Martínez- Jerónimo, Cesar Mateo Flores-Ortíz
A biomass production process including two stages, heterotrophy/photoinduction (TSHP), was developed to improve biomass and lutein production by the green microalgae Scenedesmus incrassatulus. To determine the effects of different nitrogen sources (yeast extract and urea) and temperature in the heterotrophic stage, experiments using shake flask cultures with glucose as the carbon source were carried out. The highest biomass productivity and specific pigment concentrations were reached using urea+vitamins (U+V) at 30°C...
September 16, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28922645/spectrophotometric-determination-of-fluoxetine-by-molecularly-imprinted-polypyrrole-and-optimization-by-experimental-design-artificial-neural-network-and-genetic-algorithm
#11
Azizollah Nezhadali, Maryam Omidvar Motlagh, Samira Sadeghzadeh
A selective method based on molecularly imprinted polymer (MIP) solid-phase extraction (SPE) using UV-Vis spectrophotometry as a detection technique was developed for the determination of fluoxetine (FLU) in pharmaceutical and human serum samples. The MIPs were synthesized using pyrrole as a functional monomer in the presence of FLU as a template molecule. The factors that affecting the preparation and extraction ability of MIP such as amount of sorbent, initiator concentration, the amount of monomer to template ratio, uptake shaking rate, uptake time, washing buffer pH, take shaking rate, Taking time and polymerization time were considered for optimization...
September 13, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28921965/mycelium-enhanced-bacterial-degradation-of-organic-pollutants-under-bioavailability-restrictions
#12
Rungroch Sungthong, Margalida Tauler, Magdalena Grifoll, Jose-Julio Ortega-Calvo
This work examines the role of mycelia in enhancing the degradation by attached bacteria of organic pollutants that have poor bioavailability. Two oomycetes, Pythium oligandrum and Pythium aphanidermatum, were selected as producers of mycelial networks, while Mycobacterium gilvum VM552 served as a model polycyclic aromatic hydrocarbon (PAH)-degrading bacterium. The experiments consisted of bacterial cultures exposed to a non-disturbed non-aqueous phase liquid (NAPL) layer containing a heavy fuel spiked with 14C-labeled phenanthrene that were incubated in the presence or absence of the mycelia of the oomycetes in both shaking and static conditions...
September 18, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28921479/erratum-to-development-of-a-circulation-direct-sampling-and-monitoring-system-for-o2-and-co2-concentrations-in-the-gas-liquid-phases-of-shake%C3%A2-flask-systems-during-microbial-cell-culture
#13
Masato Takahashi, Yoshisuke Sawada, Hideki Aoyagi
Following publication of the original article (Takahashi et al. 2017), the authors reported that there was a mistake in the legend of Figure 2.
September 18, 2017: AMB Express
https://www.readbyqxmd.com/read/28920431/isolation-and-characterization-of-a-novel-highly-selective-astaxanthin-producing-marine-bacterium
#14
Dalal Asker
A high throughput screening approach for astaxanthin-producing bacteria led to the discovery of a novel highly selective astaxanthin-producing marine bacterium (strain N-5). Phylogenetic analysis based on partial 16S rRNA gene and phenotypic metabolic testing indicated it belongs to the genus Brevundimonas. Therefore, it was designated as Brevundimonas sp. strain N-5. To identify and quantify carotenoids produced by strain N-5, HPLC-DAD and HPLC-MS methods were used. The culture conditions including media, shaking and time had significant effects on cell growth and carotenoids production including astaxanthin...
September 18, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28919926/sustaining-fermentation-in-high-gravity-ethanol-production-by-feeding-yeast-to-a-temperature-profiled-multifeed-simultaneous-saccharification-and-co-fermentation-of-wheat-straw
#15
Johan O Westman, Ruifei Wang, Vera Novy, Carl Johan Franzén
BACKGROUND: Considerable progress is being made in ethanol production from lignocellulosic feedstocks by fermentation, but negative effects of inhibitors on fermenting microorganisms are still challenging. Feeding preadapted cells has shown positive effects by sustaining fermentation in high-gravity simultaneous saccharification and co-fermentation (SSCF). Loss of cell viability has been reported in several SSCF studies on different substrates and seems to be the main reason for the declining ethanol production toward the end of the process...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28919878/postural-control-in-bilateral-vestibular-failure-its-relation-to-visual-proprioceptive-vestibular-and-cognitive-input
#16
Andreas Sprenger, Jann F Wojak, Nico M Jandl, Christoph Helmchen
Patients with bilateral vestibular failure (BVF) suffer from postural and gait unsteadiness with an increased risk of falls. The aim of this study was to elucidate the differential role of otolith, semicircular canal (SSC), visual, proprioceptive, and cognitive influences on the postural stability of BVF patients. Center-of-pressure displacements were recorded by posturography under six conditions: target visibility; tonic head positions in the pitch plane; horizontal head shaking; sensory deprivation; dual task; and tandem stance...
2017: Frontiers in Neurology
https://www.readbyqxmd.com/read/28918584/fed-batch-strategies-for-production-of-pha-using-a-native-isolate-of-halomonas-venusta-kt832796-strain
#17
Angelina Stanley, H N Punil Kumar, Sarma Mutturi, S V N Vijayendra
In this study, polyhydroxyalkanoates (PHA) accumulation by Halomonas venusta KT832796, a moderate halophilic bacteria isolated from marine source was studied. Both nutritional requirements and process parameters for submerged cultivation of the organism in bioreactor have been standardized. From the shake flask studies, glucose and ammonium citrate as carbon and nitrogen source produced maximum PHA at a ratio 20 with 3.52 g/L of dry cell weight and 70.56% of PHA content. However, ammonium sulfate as the nitrogen source was found to be more suitable for fed-batch cultivation...
September 16, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28916461/metabolic-engineering-of-escherichia-coli-for-the-synthesis-of-the-quadripolymer-poly-glycolate-co-lactate-co-3-hydroxybutyrate-co-4-hydroxybutyrate-from-glucose
#18
Zheng-Jun Li, Kangjian Qiao, Xue-Mei Che, Gregory Stephanopoulos
Escherichia coli was metabolically engineered to effectively produce a series of biopolymers consisted of four types of monomers including glycolate, lactate, 3-hydroxybutyrate and 4-hydroxybutyrate from glucose as the carbon source. The biosynthetic route of novel quadripolymers was achieved by the overexpression of a range of homologous and heterologous enzymes including isocitrate lyase, isocitrate dehydrogenase kinase/phosphatase, glyoxylate/hydroxypyruvate reductase, propionyl-CoA transferase, β-ketothiolase, acetoacetyl-CoA reductase, succinate semialdehyde dehydrogenase, 4-hydroxybutyrate dehydrogenase, CoA transferase and PHA synthase...
September 13, 2017: Metabolic Engineering
https://www.readbyqxmd.com/read/28915746/single-double-and-triple-auger-decays-from-1s-shake-up-states-of-the-oxygen-molecule
#19
T Kaneyasu, T Odagiri, M Nakagawa, R Mashiko, H Tanaka, J Adachi, Y Hikosaka
The single, double, and triple Auger decays from the 1s shake-up states of O2 have been studied using a multi-electron coincidence method. Efficient populations of two-hole final states are observed in single Auger decays of the π-π* shake-up states, which is understood as a characteristic property of the Auger transitions from shake-up states of an open-shell molecule. The O2(3+) populations formed by double Auger decays show similar profiles for both the O1s(-1) and shake-up states, which is due to the contributions from cascade double Auger processes...
September 14, 2017: Journal of Chemical Physics
https://www.readbyqxmd.com/read/28914347/intermetatarsal-bursa-primary-synovial-chondromatosis-case-report-and-review-of-the-literature
#20
Manuel Trevino, Shaked Laks, Lisa Kafchinski, Dinesh K Sundarakumar, Crysela M Smith
Primary synovial chondromatosis is a benign neoplastic process, occurring mostly in large joints, more rarely in tendon sheaths, and extremely uncommonly in bursae. We describe a patient with primary synovial chondromatosis arising in the fourth intermetatarsal bursa. Knowledge of the bursal anatomy of the forefoot, and of characteristic imaging findings and the pathogenesis of synovial chondromatosis, is essential in including this uncommon entity in the differential when occurring in unusual locations.
September 15, 2017: Skeletal Radiology
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