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https://www.readbyqxmd.com/read/28432542/limb-shaking-tia-on-the-borders-of-evidence-based-medicine
#1
Antonio Carolei, Pietro Amedeo Modesti
No abstract text is available yet for this article.
April 21, 2017: Internal and Emergency Medicine
https://www.readbyqxmd.com/read/28430025/synthesis-of-dopamine-in-e-coli-using-plasmid-based-expression-system-and-its-marked-effect-on-host-growth-profiles
#2
Arunangshu Das, Anita Verma, Krishna J Mukherjee
L-Dopa and dopamine are important pathway intermediates towards the synthesis of catecholamine like epinephrine and norepinephrine from amino acid L-tyrosine. Dopamine, secreted from dopaminergic nerve cells, serves as an important neurotransmitter. We report the synthesis of dopamine by extending the aromatic amino acid pathway of E.coli DH5α by expression of 4-hydroxyphenylacetate-3-hydrolase (HpaBC) from E.coli and an engineered dopa decarboxylase (DDC) from pig kidney cell. The activity of HpaBC and DDC require 200 µM iron supplementation and 50 µM vitamin B6 respectively as additives to the growth media...
April 21, 2017: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/28427629/development-of-a-novel-folate-modified-nanobubbles-with-improved-targeting-ability-to-tumor-cells
#3
Sujuan Duan, Lu Guo, Dandan Shi, Mengmeng Shang, Dong Meng, Jie Li
Conjugation of folate (FOL) to nanobubbles could enhance the selective targeting to tumors expressing high levels of folate receptor (FR). To further improve the selective targeting ability of FOL-modified nanobubbles, a novel FOL-targeted nanobubble ((FOL)2-NB) with increasing FOL content (accomplished by linking two FOL molecules per DSPE-PEG2000 chain) was synthesized, through the methods of mechanical shaking and low-speed centrifugation based on lipid-stabilized perfluoropropane. The bubble size and distribution range were measured by dynamic light scattering (DLS)...
July 2017: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/28426133/quantitative-phase-microscopy-spatial-signatures-of-cancer-cells
#4
Darina Roitshtain, Lauren Wolbromsky, Evgeny Bal, Hayit Greenspan, Lisa L Satterwhite, Natan T Shaked
We present cytometric classification of live healthy and cancerous cells by using the spatial morphological and textural information found in the label-free quantitative phase images of the cells. We compare both healthy cells to primary tumor cells and primary tumor cells to metastatic cancer cells, where tumor biopsies and normal tissues were isolated from the same individuals. To mimic analysis of liquid biopsies by flow cytometry, the cells were imaged while unattached to the substrate. We used low-coherence off-axis interferometric phase microscopy setup, which allows a single-exposure acquisition mode, and thus is suitable for quantitative imaging of dynamic cells during flow...
April 20, 2017: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/28421142/plackett-burman-design-for-rgilcc1-laccase-activity-enhancement-in-pichia-pastoris-concentrated-enzyme-kinetic-characterization
#5
Edwin D Morales-Álvarez, Claudia M Rivera-Hoyos, Ángela M Cardozo-Bernal, Raúl A Poutou-Piñales, Aura M Pedroza-Rodríguez, Dennis J Díaz-Rincón, Alexander Rodríguez-López, Carlos J Alméciga-Díaz, Claudia L Cuervo-Patiño
Laccases are multicopper oxidases that catalyze aromatic and nonaromatic compounds with concomitant reduction of molecular oxygen to water. They are of great interest due to their potential biotechnological applications. In this work we statistically improved culture media for recombinant GILCC1 (rGILCC1) laccase production at low scale from Ganoderma lucidum containing the construct pGAPZαA-GlucPost-Stop in Pichia pastoris. Temperature, pH stability, and kinetic parameter characterizations were determined by monitoring concentrate enzyme oxidation at different ABTS substrate concentrations...
2017: Enzyme Research
https://www.readbyqxmd.com/read/28420336/reduced-changes-in-protein-compared-to-mrna-levels-across-non-proliferating-tissues
#6
Kobi Perl, Kathy Ushakov, Yair Pozniak, Ofer Yizhar-Barnea, Yoni Bhonker, Shaked Shivatzki, Tamar Geiger, Karen B Avraham, Ron Shamir
BACKGROUND: The quantitative relations between RNA and protein are fundamental to biology and are still not fully understood. Across taxa, it was demonstrated that the protein-to-mRNA ratio in steady state varies in a direction that lessens the change in protein levels as a result of changes in the transcript abundance. Evidence for this behavior in tissues is sparse. We tested this phenomenon in new data that we produced for the mouse auditory system, and in previously published tissue datasets...
April 18, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28416394/moderate-reagent-mixing-on-an-orbital-shaker-reduces-the-incubation-time-of-enzyme-linked-immunosorbent-assay
#7
Saroj Kumar, Rajesh Ahirwar, Ishita Rehman, Pradip Nahar
Rapid diagnostic tests can be developed using ELISA for detection of diseases in emergency conditions. Conventional ELISA takes 1-2 days, making it unsuitable for rapid-diagnostics. Here, we report the effect of reagents mixing via shaking or vortexing on the assay timing of ELISA. A 48-min protocol of ELISA involving 12-min incubations with reagent mixing at 750 rpm of for every step was optimized. Contrary to this, time-optimized control ELISA performed without mixing produced similar results in 8 h, leaving a time gain of 7 h using the developed protocol...
April 14, 2017: Analytical Biochemistry
https://www.readbyqxmd.com/read/28414677/a-rare-case-of-tropical-pyomysitis-acquired-on-vacation-in-a-healthy-male
#8
K Poole, T Tran
INTRODUCTION: Pyomyositis has traditionally been considered a tropical disease. Increasing prevalence has been observed in more northern climates, especially with HIV, immunosuppression, and IV drug abuse as predisposing factors. Pyomyositis has the tendency to mimic other conditions, such as muscle contusion, necrotizing fasciitis, or septic arthritis. The rarity of this disease and non-specificity of its symptoms often lead to delay in appropriate diagnosis and treatment. CASE: 24-year-old man, seen 2 days prior in orthopedics clinic and the Emergency Department for persistent gluteal pain thought to be due to right-sided sciatica, was admitted following worsening pain, diffuse generalized weakness with polyarthralgias and myalgias These symptoms were associated with night sweats, shaking chills, and difficulty walking...
March 2017: Journal of the Louisiana State Medical Society: Official Organ of the Louisiana State Medical Society
https://www.readbyqxmd.com/read/28414263/effective-enhancement-of-polylactic-acid-pla-degrading-enzyme-production-by-amycolatopsis-sp-strain-scm_mk2-4-using-statistical-and-one-factor-at-a-time-ofat-approaches
#9
Watsana Penkhrue, Apinun Kanpiengjai, Chartchai Khanongnuch, Kazuo Masaki, Wasu Pathom-Aree, Winita Punyodom, Saisamorn Lumyong
This study aims to find the optimal medium and conditions for polylactic acid (PLA)-degrading enzyme production by Amycolatopsis sp. SCM_MK2-4. Screening of the most effective components in the enzyme production medium by PBD revealed that the silk cocoon and PLA film were the most significant variables enhancing the PLA-degrading enzyme production. After a RSM, a maximum amount of PLA-degrading enzyme activity at 0.74 U mL(-1) was predicted and successfully validated at 95% after 0.39% (w/v) silk cocoon and 1...
April 17, 2017: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/28414213/immobilization-and-stabilization-of-acylase-on-carboxylated-polyaniline-nanofibers-for-highly-effective-antifouling-application-via-quorum-quenching
#10
Jeongjoon Lee, Inseon Lee, Jahyun Nam, Dong Soo Hwang, Kyung-Min Yeon, Jungbae Kim
Acylase was immobilized and stabilized on carboxylated polyaniline nanofibers (cPANFs) for the development of antifouling nanobiocatalysis with high enzyme loading and stability. Acylase was immobilized via three different approaches: covalent attachment (CA), enzyme coating (EC), and magnetically separable enzyme precipitate coating (Mag-EPC). The enzyme activity per unit weight of cPANFs of Mag-EPC was 75 and 300 times higher than those of CA and EC, respectively, representing the improved enzyme loading in the form of Mag-EPC...
April 17, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28410897/efficient-production-of-antibody-fab-fragment-by-transient-gene-expression-in-insect-cells
#11
Keita Mori, Hirotsugu Hamada, Takafumi Ogawa, Yuki Ohmuro-Matsuyama, Tomohisa Katsuda, Hideki Yamaji
Transient gene expression allows a rapid production of diverse recombinant proteins in early-stage preclinical and clinical developments of biologics. Insect cells have proven to be an excellent platform for the production of functional recombinant proteins. In the present study, the production of an antibody Fab fragment by transient gene expression in lepidopteran insect cells was investigated. The DNA fragments encoding heavy-chain (Hc; Fd fragment) and light-chain (Lc) genes of an Fab fragment were individually cloned into the plasmid vector pIHAneo, which contained the Bombyx mori actin promoter downstream of the B...
April 11, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28409974/picosecond-control-of-quantum-dot-laser-emission-by-coherent-phonons
#12
T Czerniuk, D Wigger, A V Akimov, C Schneider, M Kamp, S Höfling, D R Yakovlev, T Kuhn, D E Reiter, M Bayer
A picosecond acoustic pulse can be used to control the lasing emission from semiconductor nanostructures by shifting their electronic transitions. When the active medium, here an ensemble of (In,Ga)As quantum dots, is shifted into or out of resonance with the cavity mode, a large enhancement or suppression of the lasing emission can dynamically be achieved. Most interesting, even in the case when gain medium and cavity mode are in resonance, we observe an enhancement of the lasing due to shaking by coherent phonons...
March 31, 2017: Physical Review Letters
https://www.readbyqxmd.com/read/28409780/compact-interferometric-module-for-full-field-interferometric-phase-microscopy-with-low-spatial-coherence-illumination
#13
Amit Nativ, Natan T Shaked
We propose a compact and external off-axis interferometric module that can achieve interference with low spatial coherence illumination over the entire field of view. The interferometer is easy to align and stable and can be connected to the output of an existing microscope illuminated with a low spatial coherence light source, thus allowing quantitative phase imaging with a low degree of spatial noise. We demonstrate the imaging and the interference properties of the proposed interferometric module and use it for quantitative phase imaging of reflective samples...
April 15, 2017: Optics Letters
https://www.readbyqxmd.com/read/28407249/healthcare-quality-a-concept-analysis
#14
Angela Allen-Duck, Jennifer C Robinson, Mary W Stewart
Worsening quality indicators of health care shake public trust. Although safety and quality of care in hospitals can be improved, healthcare quality remains conceptually and operationally vague. Therefore, the aim of this analysis is to clarify the concept of healthcare quality. Walker and Avant's method of concept analysis, the most commonly used in nursing literature, provided the framework. We searched general and medical dictionaries, public domain websites, and 5 academic literature databases. Search terms included health care and quality, as well as healthcare and quality...
April 13, 2017: Nursing Forum
https://www.readbyqxmd.com/read/28402216/a-novel-and-efficient-immunoglobulin-y-extraction-method-using-poloxamer-polyethylene-glycol
#15
Cui Li, Hao Ren, Rüdiger Schade, Xiaoying Zhang
Although many IgY extraction methods (such as polyethylene glycol (PEG) precipitation method, octanoic acid method, water dilution method, etc.) have been established, there is still industrial drive and real need in developing scale-up IgY production methods. Some previous studies have reported that poloxamer degreasing method shows very good result in IgY extraction from egg yolk with high degreasing speed, harmlessness, simpleness in operation and minimal effect on antibody titer. In this study, we developed a new method, poloxamer-polyethylene glycol (PEG) method, to obtain functional IgY with high purity and yield...
April 12, 2017: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/28401465/metabolism-of-pyrene-through-phthalic-acid-pathway-by-enriched-bacterial-consortium-composed-of-pseudomonas-burkholderia-and-rhodococcus-pbr
#16
Sagar Vaidya, Kunal Jain, Datta Madamwar
Polycyclic aromatic hydrocarbons (PAHs) are highly recalcitrant compounds due to their high hydrophobicity and tendency to partition in organic phase of soils. Pyrene is a high-molecular weight PAH, which has human health concerns. In the present study, a bacterial consortium, PBR, was developed from a long-term polluted site, viz., Amlakhadi, Ankleshwar, Gujarat, for effective degradation of pyrene. The consortium effectively metabolized pyrene as a sole source of carbon and energy. The consortium comprised three bacterial species, Pseudomonas sp...
May 2017: 3 Biotech
https://www.readbyqxmd.com/read/28401428/not-all-intermittent-shakings-are-epilepsy
#17
Cindy Tiseo, Raffaele Ornello, Diana Degan, Francesca Notturno
No abstract text is available yet for this article.
April 11, 2017: Internal and Emergency Medicine
https://www.readbyqxmd.com/read/28399991/in-package-inhibition-of-e-%C3%A2-coli-o157-h7-on-bulk-romaine-lettuce-using-cold-plasma
#18
Sea C Min, Si Hyeon Roh, Brendan A Niemira, Glenn Boyd, Joseph E Sites, Joseph Uknalis, Xuetong Fan
Dielectric barrier discharge atmospheric cold plasma (DACP) treatment was evaluated for the inactivation of Escherichia coli O157:H7, surface morphology, color, carbon dioxide generation, and weight loss of bulk Romaine lettuce in a commercial plastic clamshell container. The lettuce samples were packed in a model bulk packaging configuration (three rows with either 1, 3, 5, or 7 layers) in the container and treated by DACP (42.6 kV, 10 min). DACP treatment reduced the number of E. coli O157:H7 in the leaf samples in the 1-, 3-, and 5-layer configurations by 0...
August 2017: Food Microbiology
https://www.readbyqxmd.com/read/28395489/-frequency-characteristics-of-the-semicicular-canals-damage-in-patients-with-meniere-disease
#19
K X Xu, T S Chen, W Wang, S S Li, Q Liu, C Wen, X Han, P Lin
Objective: To evaluate semicircular canal damage and its frequency characteristics in Meniere disease patients. Methods: Sixty-seven Meniere disease patients accepted the video-head impulse test(vHIT)which responses to semicircular canal function of high frequency area, head shaking test(HST) which responses to semicircular canal function of middle frequency area, and caloric test(CT) which responses to semicircular canal function of low frequency area.Preferences were recorded including the video head impulse test gain (vHIT-G), head shaking nystagmus (HSN) and parameters of the unilateral weakness (UW) as observation index, and results of all the three tests were analyzed according to Meniere disease patients...
March 7, 2017: Zhonghua Er Bi Yan Hou Tou Jing Wai Ke za Zhi, Chinese Journal of Otorhinolaryngology Head and Neck Surgery
https://www.readbyqxmd.com/read/28393487/poly-3-hydroxybutyrate-co-3-hydroxyvalerate-production-from-biodiesel-by-product-and-propionic-acid-by-mutant-strains-of-pandoraea-sp
#20
Fabrício C de Paula, Jonas Contiero, Carolina B C de Paula, José Gregório C Gomez, Alexander Steinbüchel
Pandoraea sp. MA03 wild type strain was subjected to UV mutation in order to obtain mutants unable to grow on propionic acid (PA) but still able to produce poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)] from glycerol and PA at high 3HV yields. In shake flask experiments, mutant prp25 was selected from 52 mutants affected in the propionate metabolism exhibiting a conversion rate of PA into 3HV units of 0.78 gg(-1) . The utilization of crude glycerol (CG) plus PA or valeric acid resulted in a copolymer with 3HV contents varying from 21...
April 10, 2017: Biotechnology Progress
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