keyword
MENU ▼
Read by QxMD icon Read
search

Shaking

keyword
https://www.readbyqxmd.com/read/28210752/biodegradation-of-diesel-crude-oil-and-spent-lubricating-oil-by-soil-isolates-of-bacillus-spp
#1
Maddela Naga Raju, Rodriguez Leo, Sanaguano Salguero Herminia, Ricardo Ernesto Burgos Morán, Kadiyala Venkateswarlu, Scalvenzi Laura
Two species of Bacillus, B. thuringiensis B3 and B. cereus B6, isolated from crude oil-contaminated sites in Ecuador, were tested for their capability in degrading polycyclic aromatic hydrocarbons (PAHs) in diesel (shake-flask), and to remove total petroleum hydrocarbons (TPHs) from crude oil- or spent lubricating oil-polluted soils (plot-scale). TPHs and PAHs were analyzed by Gas chromatography-Flame ionization detector (GC-FID) and High performance liquid chromatography (HPLC), respectively. Degradation percentages of PAHs by strain B6 were in the range of 11-83 after 30 days...
February 16, 2017: Bulletin of Environmental Contamination and Toxicology
https://www.readbyqxmd.com/read/28209146/heterologous-expression-of-aspergillus-aculeatus-endo-polygalacturonase-in-pichia-pastoris-by-high-cell-density-fermentation-and-its-application-in-textile-scouring
#2
Dede Abdulrachman, Paweena Thongkred, Kanokarn Kocharin, Monthon Nakpathom, Buppha Somboon, Nootsara Narumol, Verawat Champreda, Lily Eurwilaichitr, Antonius Suwanto, Thidarat Nimchua, Duriya Chantasingh
BACKGROUND: Removal of non-cellulosic impurities from cotton fabric, known as scouring, by conventional alkaline treatment causes environmental problems and reduces physical strength of fabrics. In this study, an endo-polygalacturonase (EndoPG) from Aspergillus aculeatus produced in Pichia pastoris was evaluated for its efficiency as a bioscouring agent while most current bioscouring process has been performed using crude pectinase preparation. RESULTS: The recombinant EndoPG exhibited a specific activity of 1892...
February 16, 2017: BMC Biotechnology
https://www.readbyqxmd.com/read/28202353/the-potential-clinical-promise-of-multimodality-metronomic-chemotherapy-revealed-by-preclinical-studies-of-metastatic-disease
#3
Robert S Kerbel, Yuval Shaked
We present a rationale for further clinical development and assessment of metronomic chemotherapy on the basis of unexpected results obtained in translational mouse models of cancer involving treatment of advanced metastatic disease. Historically, mouse cancer therapy models have been dominated by treating established primary tumors or early stage low volume microscopic disease. Treatment of primary tumors is also almost always the case when using genetically engineered mouse models (GEMMS) of cancer or patient-derived xenografts (PDXs)...
February 12, 2017: Cancer Letters
https://www.readbyqxmd.com/read/28197999/parallel-experimental-design-and-multivariate-analysis-provides-efficient-screening-of-cell-culture-media-supplements-to-improve-biosimilar-product-quality
#4
David Brühlmann, Michael Sokolov, Alessandro Butté, Markus Sauer, Jürgen Hemberger, Jonathan Souquet, Hervé Broly, Martin Jordan
Rational and high-throughput optimization of mammalian cell culture media has a great potential to modulate recombinant protein product quality. We present a process design method based on parallel design-of-experiment (DoE) of CHO fed-batch cultures in 96-deepwell plates to modulate monoclonal antibody (mAb) glycosylation using medium supplements. To reduce risk of losing valuable information in an intricate joint screening, 17 compounds were separated into five different groups, considering their mode of biological action...
February 15, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28197775/individual-indicators-of-appropriate-hypnotic-level-during-propofol-anesthesia-highest-alpha-power-and-effect-site-concentrations-of-propofol-at-loss-of-response
#5
Hongling Kang, Hassan Mamdouh Hassan Mohamed, Masaki Takashina, Takahiko Mori, Yuji Fujino, Satoshi Hagihira
BACKGROUND: Electroencephalogram (EEG) waveforms vary widely among individuals, this decreases the usefulness of BIS™ monitors for assessing the effects of propofol. Practically, anesthesia is only seen as too deep when evidence of burst-suppression is seen. We designed an experiment to help towards better assessment of individual anesthetic needs. First, to mark the Ce (effect-site concentration) of propofol at loss of response to calling name and gently shaking shoulders (LOR), we defined Ce-LOR...
February 14, 2017: Journal of Anesthesia
https://www.readbyqxmd.com/read/28190553/cervical-spine-imaging-for-young-children-with-inflicted-trauma-expanding-the-injury-pattern
#6
Joanne Baerg, Arul Thirumoorthi, Rosemary Vannix, Asma Taha, Amy Young, Alexander Zouros
AIM: The purpose of this study was to document the incidence and pattern of cervical spine (c-spine) injuries in children below 36months with inflicted trauma. METHODS: An IRB approved, prospective cohort study was performed between July 2011 and January 2016. Inclusion criteria were: age below 36months, loss of consciousness after inflicted trauma, and one initial head computed tomography finding: a subdural, intraventricular, intraparenchymal, subarachnoid hemorrhage, diffuse axonal injury, hypoxic injury, or cerebral edema...
January 30, 2017: Journal of Pediatric Surgery
https://www.readbyqxmd.com/read/28188692/bioreactor-scale-up-and-protein-product-quality-characterization-of-piggybac-transposon-derived-cho-pools
#7
Yashas Rajendra, Sowmya Balasubramanian, Robert B Peery, James R Swartling, Neil A McCracken, Dawn L Norris, Christopher C Frye, Gavin C Barnard
Chinese hamster ovary (CHO) cells remain the most popular host for the production of biopharmaceutical drugs, particularly monoclonal antibodies (mAbs), bispecific antibodies and Fc-fusion proteins. Creating and characterizing the stable CHO clonally-derived cell lines (CDCLs) needed to manufacture these therapeutic proteins is a lengthy and laborious process. Therefore, CHO pools have increasingly been used to rapidly produce protein to support and enable preclinical drug development. We recently described the generation of CHO pools yielding mAb titers as high as 7...
February 11, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/28188607/enhanced-skin-permeation-of-glabridin-using-eutectic-mixture-based-nanoemulsion
#8
Chen Liu, Jin Hu, Hong Sui, Qipeng Zhao, Xia Zhang, Wenping Wang
This study aimed to investigate the performance of the eutectic mixture of menthol and camphor (1:1, w/w) in nanoemulsion formulation for enhanced transdermal penetration of water-insoluble glabridin. Glabridin solubility in different media was determined by a shaking bottle method. The pseudoternary phase diagrams of the oil phase (drug-loaded eutectic mixture or IPM), the surfactant (Tween 80:glycerol = 2:1, w/w), and water were constructed using the aqueous titration method. The obtained glabridin nanoemulsions were characterized and compared on their particle sizes, in vitro and in vivo penetration performance on rat skin, and storage stability...
February 10, 2017: Drug Delivery and Translational Research
https://www.readbyqxmd.com/read/28186124/algal-cell-response-to-pulsed-waved-stimulation-and-its-application-to-increase-algal-lipid-production
#9
Oleksandra Savchenko, Jida Xing, Xiaoyan Yang, Quanrong Gu, Mohamed Shaheen, Min Huang, Xiaojian Yu, Robert Burrell, Prabir Patra, Jie Chen
Generating renewable energy while sequestering CO2 using algae has recently attracted significant research attention, mostly directing towards biological methods such as systems biology, genetic engineering and bio-refining for optimizing algae strains. Other approaches focus on chemical screening to adjust culture conditions or culture media. We report for the first time the physiological changes of algal cells in response to a novel form of mechanical stimulation, or a pulsed wave at the frequency of 1.5 MHz and the duty cycle of 20%...
February 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28178291/long-term-trends-in-the-honeybee-whooping-signal-revealed-by-automated-detection
#10
Michael Ramsey, Martin Bencsik, Michael I Newton
It is known that honeybees use vibrational communication pathways to transfer information. One honeybee signal that has been previously investigated is the short vibrational pulse named the 'stop signal', because its inhibitory effect is generally the most accepted interpretation. The present study demonstrates long term (over 9 months) automated in-situ non-invasive monitoring of a honeybee vibrational pulse with the same characteristics of what has previously been described as a stop signal using ultra-sensitive accelerometers embedded in the honeycomb located at the heart of honeybee colonies...
2017: PloS One
https://www.readbyqxmd.com/read/28177416/effects-of-biochar-on-dispersibility-of-colloids-in-agricultural-soils
#11
K G I D Kumari, Per Moldrup, Marcos Paradelo, Lars Elsgaard, Lis W de Jonge
The mobility of water-dispersible colloids (WDCs) in soil may be influenced by soil management practices such as organic soil amendments. Biochar has recently been promoted as a useful soil amendment, and extensive research has been devoted to investigating its effects on soil macroscopic properties and functions. However, there is limited understanding of the effects of biochar application on micro-scale particle dynamics. We conducted a field study to investigate the effects of the application of birch ( spp...
January 2017: Journal of Environmental Quality
https://www.readbyqxmd.com/read/28176287/metal-ions-removal-using-nano-oxide-pyrolox%C3%A2-material
#12
A Gładysz-Płaska, E Skwarek, T M Budnyak, D Kołodyńska
The paper presents the use of Pyrolox™ containing manganese nano oxides used for the removal of Cu(II), Zn(II), Cd(II), and Pb(II) as well as U(VI) ions. Their concentrations were analyzed using the atomic absorption spectrometer SpectrAA 240 FS (Varian) as well as UV-vis method. For this purpose the static kinetic and equilibrium studies were carried out using the batch technique. The effect of solution pH, shaking time, initial metal ion concentrations, sorbent dosage, and temperature was investigated. The equilibrium data were analyzed using the sorption isotherm models proposed by Freundlich, Langmuir-Freundlich, Temkin, and Dubinin-Radushkevich...
December 2017: Nanoscale Research Letters
https://www.readbyqxmd.com/read/28176140/prospecting-agro-waste-cocktail-supplementation-for-cellulase-production-by-a-newly-isolated-thermophilic-b-licheniformis-2d55
#13
Muinat Olanike Kazeem, Umi Kalsom Md Shah, Azhari Samsu Baharuddin, Nor' Aini AbdulRahman
Bacteria isolated from thermophilic environment that can produce cellulase as well as utilise agro-waste biomass have a high potential for developing thermostable cellulase required in the biofuel industry. The cost for cellulase represents a significant challenge in converting lignocellulose to fermentable sugars for biofuel production. Among three potential bacteria examined, Bacillus licheniformis 2D55 (accession no. KT799651) was found to produce the highest cellulolytic activity (CMCase 0.33 U/mL and FPase 0...
February 7, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28174563/metabolic-flexibility-of-yarrowia-lipolytica-growing-on-glycerol
#14
Michael Egermeier, Hannes Russmayer, Michael Sauer, Hans Marx
The yeast Yarrowia lipolytica is a fascinating microorganism with an amazing metabolic flexibility. This yeast grows very well on a wide variety of carbon sources from alkanes over lipids, to sugars and glycerol. Y. lipolytica accumulates a wide array of industrially relevant metabolites. It is very tolerant to many environmental factors, above all the pH value. It grows perfectly well over a wide pH range, but it has been described, that the pH has a decisive influence on the metabolite pattern accumulated by this yeast...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28168200/production-and-partial-characterization-of-%C3%AE-amylase-enzyme-from-bacillus-sp-bcc-01-50-and-potential-applications
#15
Altaf Ahmed Simair, Abdul Sattar Qureshi, Imrana Khushk, Chaudhry Haider Ali, Safia Lashari, Muhammad Aqeel Bhutto, Ghulam Sughra Mangrio, Changrui Lu
Amylase is an industrially important enzyme and applied in many industrial processes such as saccharification of starchy materials, food, pharmaceutical, detergent, and textile industries. This research work deals with the optimization of fermentation conditions for α-amylase production from thermophilic bacterial strain Bacillus sp. BCC 01-50 and characterization of crude amylase. The time profile of bacterial growth and amylase production was investigated in synthetic medium and maximum enzyme titer was observed after 60 h...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28166912/tuberculosis-knowledge-and-attitude-in-aspiring-doctors-and-nurses-is-it-time-for-our-tb-teaching-methods-to-evolve
#16
Preetam Rajgopal Acharya, Monalisa D'Souza, Ramesh Chandra Sahoo
BACKGROUND: India accounts for nearly 24% of all the new tuberculosis (TB) cases globally. A good core knowledge and a positive outlook towards TB patients among our aspiring doctors and nurses are necessities for India to meet the Sustainable Development Goals (SDG) proposed by the WHO as a part of its post-2015 global TB strategy and to successfully combat the newer challenges posed by this disease in the future. AIMS: To evaluate knowledge related to transmission, prevention and treatment of tuberculosis amongst medical and nursing students...
January 2017: Indian Journal of Tuberculosis
https://www.readbyqxmd.com/read/28166538/single-cell-spatial-reconstruction-reveals-global-division-of-labour-in-the-mammalian-liver
#17
Keren Bahar Halpern, Rom Shenhav, Orit Matcovitch-Natan, Beáta Tóth, Doron Lemze, Matan Golan, Efi E Massasa, Shaked Baydatch, Shanie Landen, Andreas E Moor, Alexander Brandis, Amir Giladi, Avigail Stokar-Avihail, Eyal David, Ido Amit, Shalev Itzkovitz
The mammalian liver consists of hexagon-shaped lobules that are radially polarized by blood flow and morphogens. Key liver genes have been shown to be differentially expressed along the lobule axis, a phenomenon termed zonation, but a detailed genome-wide reconstruction of this spatial division of labour has not been achieved. Here we measure the entire transcriptome of thousands of mouse liver cells and infer their lobule coordinates on the basis of a panel of zonated landmark genes, characterized with single-molecule fluorescence in situ hybridization...
February 16, 2017: Nature
https://www.readbyqxmd.com/read/28162474/-244-exploration-of-racial-pain-disparities-the-role-of-urban-environmental-stressors
#18
R Arnold, D Shaked, E Boyd, M Evans, A Zonderman, S Waldstein, R Quiton
No abstract text is available yet for this article.
April 2016: Journal of Pain: Official Journal of the American Pain Society
https://www.readbyqxmd.com/read/28161126/enhancing-silymarin-fractionation-using-the-conductor-like-screening-model-for-real-solvents
#19
Emma C Brace, Abigail S Engelberth
A significant hurdle for discovery of plant-derived products is the numerous trial-and-error experiments required to develop an effective purification strategy. To overcome the experimental burden, a quantum mechanics-based molecular modeling approach - known as the COnductor-like Screening Model for Real Solvents (COSMO-RS) - was used to predict a suitable two-phase solvent system to purify six silymarins from an aqueous mixture. Silymarins, a class of flavonolignans found in milk thistle (Silybum marianum L...
January 23, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28157994/camera-vibration-measurement-using-blinking-light-emitting-diode-array
#20
Kazuki Nishi, Yuichi Matsuda
We present a new method for measuring camera vibrations such as camera shake and shutter shock. This method successfully detects the vibration trajectory and transient waveforms from the camera image itself. We employ a time-varying pattern as the camera test chart over the conventional static pattern. This pattern is implemented using a specially developed blinking light-emitting-diode array. We describe the theoretical framework and pattern analysis of the camera image for measuring camera vibrations. Our verification experiments show that our method has a detection accuracy and sensitivity of 0...
January 23, 2017: Optics Express
keyword
keyword
13664
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"