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https://www.readbyqxmd.com/read/29351285/wine-yeast-phenomics-a-standardized-fermentation-method-for-assessing-quantitative-traits-of-saccharomyces-cerevisiae-strains-in-enological-conditions
#1
Emilien Peltier, Margaux Bernard, Marine Trujillo, Duyên Prodhomme, Jean-Christophe Barbe, Yves Gibon, Philippe Marullo
This work describes the set up of a small scale fermentation methodology for measuring quantitative traits of hundreds of samples in an enological context. By using standardized screw cap vessels, the alcoholic fermentation kinetics of Saccharomyces cerevisiae strains were measured by following their weight loss over the time. This dispositive was coupled with robotized enzymatic assays for measuring metabolites of enological interest in natural grape juices. Despite the small volume used, kinetic parameters and fermentation end products measured are similar with those observed in larger scale vats...
2018: PloS One
https://www.readbyqxmd.com/read/29350299/mutagenicity-cytotoxic-and-antioxidant-activities-of-ricinus-communis-different-parts
#2
Mazhar Abbas, Abid Ali, Muhammad Arshad, Asia Atta, Zahed Mehmood, Imtiaz Mahmood Tahir, Munawar Iqbal
Ricinus communis (castor plant) is a potent medicinal plant, which is commonly used in the treatment of various ailments. The present study was conducted to appraise the cytotoxicity and mutagenicity of R. communis along with antioxidant and antimicrobial activities. Cytotoxicity was evaluated by hemolytic and brine shrimp assays, whereas Ames test (TA98 and TA100) was used for mutagenicity evaluation. Plant different parts were extracted in methanol by shaking, sonication and Soxhlet extraction methods. The R...
January 19, 2018: Chemistry Central Journal
https://www.readbyqxmd.com/read/29349923/magnetic-macroporous-hydrogels-as-a-novel-approach-for-perfused-stem-cell-culture-in-3d-scaffolds-via-contactless-motion-control
#3
Lisa Rödling, Esther Magano Volz, Annamarija Raic, Katharina Brändle, Matthias Franzreb, Cornelia Lee-Thedieck
There is an urgent need for 3D cell culture systems that avoid the oversimplifications and artifacts of conventional culture in 2D. However, 3D culture within the cavities of porous biomaterials or large 3D structures harboring high cell numbers is limited by the needs to nurture cells and to remove growth-limiting metabolites. To overcome the diffusion-limited transport of such soluble factors in 3D culture, mixing can be improved by pumping, stirring or shaking, but this in turn can lead to other problems...
January 19, 2018: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/29346524/-effects-of-a-primary-care-based-multifactorial-intervention-on-physical-and-cognitive-function-in-frail-elderly-individuals-a-randomized-controlled-trial
#4
Laura Romera-Liebana, Francesc Orfila, Josep Maria Segura, Jordi Real, Maria Lluïsa Fabra, Mercedes Möller, Santiago Lancho, Anna Ramirez, Nuria Marti, Montserrat Cullell, Nuria Bastida, Dolors Martinez, Maria Giné, Patricia Cendrós, Anna Bistuer, Elena Perez, Maria Assumpta Fabregat, Gonçal Foz
Background: Detecting and managing frailty at early stages can prevent disability and other adverse outcomes. The study aim was to evaluate whether a multifactorial intervention program could modify physical and cognitive frailty parameters in elderly individuals. Methods: We conducted a multicenter, randomized, single-blind, parallel-group trial in community-living pre-frail/frail elderly individuals in Barcelona. A total of 352 patients, aged ≥65 years old with positive frailty screening, was randomized into two groups to receive a 12-week multidisciplinary intervention or usual care, with concealed allocation...
January 16, 2018: Journals of Gerontology. Series A, Biological Sciences and Medical Sciences
https://www.readbyqxmd.com/read/29346091/plenoptic-image-motion-deblurring
#5
Paramanand Chandramouli, Meiguang Jin, Daniele Perrone, Paolo Favaro
We propose a method to remove motion blur in a single light field captured with a moving plenoptic camera. Since motion is unknown, we resort to a blind deconvolution formulation, where one aims to identify both the blur point spread function and the latent sharp image. Even in the absence of motion, light field images captured by a plenoptic camera are affected by a non-trivial combination of both aliasing and defocus, which depends on the 3D geometry of the scene. Therefore, motion deblurring algorithms designed for standard cameras are not directly applicable...
April 2018: IEEE Transactions on Image Processing: a Publication of the IEEE Signal Processing Society
https://www.readbyqxmd.com/read/29343752/counting-caenorhabditis-elegans-protocol-optimization-and-applications-for-population-growth-and-toxicity-studies-in-liquid-medium
#6
Leona D Scanlan, Steven P Lund, Sanem Hosbas Coskun, Shannon K Hanna, Monique E Johnson, Christopher M Sims, Karina Brignoni, Patricia Lapasset, Elijah J Petersen, John T Elliott, Bryant C Nelson
The nematode Caenorhabditis elegans is used extensively in molecular, toxicological and genetics research. However, standardized methods for counting nematodes in liquid culture do not exist despite the wide use of nematodes and need for accurate measurements. Herein, we provide a simple and affordable counting protocol developed to maximize count accuracy and minimize variability in liquid nematode culture. Sources of variability in the counting process were identified and tested in 14 separate experiments...
January 17, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29341029/in-vitro-and-in-situ-absorption-and-metabolism-of-sesquiterpenes-from-petasites-hybridus-extracts
#7
Lucia Disch, Kristina Forsch, Beate Siewert, Jürgen Drewe, Gert Fricker
Petasites hybridus extract is used in the treatment of seasonal allergic rhinitis. The aim of this study was to evaluate the active constituent petasin and its isomers isopetasin and neopetasin (petasins) in the P. hybridus extract Ze 339 for liberation, dissolution, absorption, and metabolism. The determination of pH-dependent thermodynamic solubility was performed via the shake-flask method. Petasins exhibited a low solubility that was pH independent. In vivo, the concentration of solute drugs is decreased continuously by intestinal absorption...
January 16, 2018: Planta Medica
https://www.readbyqxmd.com/read/29339233/ftir-as-an-easy-and-fast-analytical-approach-to-follow-up-microbial-growth-during-fungal-pretreatment-of-poplar-wood-with-phanerochaete-chrysosporium
#8
I Cornet, N Wittner, G Tofani, S Tavernier
Since the determination of the fermentation kinetics is one of the main challenges in solid state fermentation, the quantitative measurement of biomass growth during microbial pretreatment by FTIR spectroscopy in Attenuated Total Reflectance mode was evaluated. Peaks at wave numbers of 1651 cm-1 and 1593 cm-1 showed to be affected during pretreatment of poplar wood particles by Phanerochaete chrysosporium MUCL 19343. Samples with different microbial biomass fractions were obtained from two different experiments, i...
January 12, 2018: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/29338250/pickering-emulsion-formation-of-paraffin-wax-in-ethanol-water-mixture-stabilized-by-primary-polymer-particles-and-wax-microspheres-thereof
#9
Zuoxu Xiao, Hongyan Cao, Xubao Jiang, Xiang Zheng Kong
Stable dispersions of paraffin wax droplets and their nano- and microspheres have broad applications. Despite intensive efforts, the production of uniform wax spheres remains a challenge. For their preparation, abundant surfactants and other additives are commonly used to stabilize the dispersions. These additives are hardly removable and entrain often adverse consequence in many applications, particularly in biological and medical applications, where microspheres with absolutely clean surface are preferred...
January 16, 2018: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/29337903/use-of-accelerometer-activity-monitors-to-detect-changes-in-pruritic-behaviors-interim-clinical-data-on-6-dogs
#10
Susan M Wernimont, Robin J Thompson, Scott L Mickelsen, Spencer C Smith, Isabella C Alvarenga, Kathy L Gross
Veterinarians and pet owners have limited ability to assess pruritic behaviors in dogs. This pilot study assessed the capacity of the Vetrax® triaxial accelerometer to measure these behaviors in six dogs with pruritus likely due to environmental allergens. Dogs wore the activity monitor for two weeks while consuming their usual pet food (baseline), then for eight weeks while consuming a veterinary-exclusive pet food for dogs with suspected non-food-related skin conditions (Hill's Prescription Diet® Derm DefenseTM Canine dry food)...
January 16, 2018: Sensors
https://www.readbyqxmd.com/read/29337886/mechanical-activation-of-adipose-tissue-and-derived-mesenchymal-stem-cells-novel-anti-inflammatory-properties
#11
Stephana Carelli, Mattia Colli, Valeriano Vinci, Fabio Caviggioli, Marco Klinger, Alfredo Gorio
The adipose tissue is a source of inflammatory proteins, such as TNF, IL-6, and CXCL8. Most of their production occurs in macrophages that act as scavengers of dying adipocytes. The application of an orbital mechanical force for 6-10 min at 97 g to the adipose tissue, lipoaspirated and treated according to Coleman procedures, abolishes the expression of TNF-α and stimulates the expression of the anti-inflammatory protein TNF-stimulated gene-6 (TSG-6). This protein had protective and anti-inflammatory effects when applied to animal models of rheumatic diseases...
January 16, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29337646/stability-of-an-aluminum-salt-adjuvanted-protein-d-conjugated-pneumococcal-vaccine-after-exposure-to-subzero-temperatures
#12
Juliette Fortpied, Florence Wauters, Christelle Rochart, Philippe Hermand, Bernard Hoet, Nicolas Moniotte, Ivo Vojtek
Accidental exposure of a vaccine containing an aluminum-salt adjuvant to temperatures below 0°C in the cold chain can lead to freeze damage. Our study evaluated the potential for freeze damage in a licensed aluminum-salt-containing protein-D-conjugated pneumococcal vaccine (PHiD-CV; Synflorix, GSK) in conditions that included static storage, single subzero-temperature excursions, and simulated air-freight transportation. Several parameters were assessed including freezing at subzero temperatures, aluminum-salt-particle size, antigen integrity and immunogenicity in the mouse...
January 16, 2018: Human Vaccines & Immunotherapeutics
https://www.readbyqxmd.com/read/29336215/non-invasive-real-time-assessment-of-cell-viability-in-a-three-dimensional-tissue
#13
Seyed Hossein Mahfouzi, Ghassem Amoabediny, Ali Doryab, Seyed Hamid Safiabadi-Tali, Mostafa Ghanei
Maintaining cell viability within 3D tissue engineering scaffolds is an essential step toward a functional tissue or organ. Assessment of cell viability in 3D scaffolds is necessary to control and optimize tissue culture process. Monitoring systems based on respiration activity of cells (e.g., oxygen consumption) have been used in various cell cultures. In this research, an online monitoring system based on respiration activity was developed to monitor cell viability within acellular lung scaffolds. First, acellular lung scaffolds were recellularized with human umbilical cord vein endothelial cells, and then, cell viability was monitored during a 5-day period...
January 16, 2018: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/29336178/evaluation-of-take-home-exposure-to-asbestos-from-handling-asbestos-contaminated-worker-clothing-following-the-abrasive-sawing-of-cement-pipe
#14
Anders Abelmann, Joshua R Maskrey, Jason T Lotter, Aaron M Chapman, Melanie D Nembhard, Jennifer S Pierce, John M Wilmoth, Richard J Lee, Dennis J Paustenbach
Although industrial uses of asbestos have declined since the 1970s, in recent years there has been a renewed interest in para-occupational ("take-home") exposure to these fibers. The aim of this study was to quantify the release of asbestos fibers, if any, during the shaking out of crocidolite- and chrysotile-contaminated clothing in a simulated at-home setting. An exposure study was conducted in which personal and area air samples were collected during the handling (i.e. shake-out) of work clothing (shirt and pants) previously worn by an operator who had cut asbestos-containing cement pipe...
January 16, 2018: Inhalation Toxicology
https://www.readbyqxmd.com/read/29335877/development-of-cyclic-amp-receptor-protein-based-artificial-transcription-factor-for-intensifying-gene-expression
#15
Peng Zhao, Wenjuan Wang, Pingfang Tian
Vector-dependent gene overexpression typically relies on an efficient operon and sufficient RNA polymerases (RNAPs). The lac (lactose) operon is a paradigm of transcription control, and cyclic AMP receptor protein (CRP) is a global regulator capable of recruiting RNAPs. However, the gap between lac operon and CRP has not been well bridged. In this work, CRP was fused to lac repressor protein (lacI) to form an artificial transcription factor (ATF) with the expectation that when LacI acted on the lacO-positioned upstream of gene of interest, the LacI-tethered CRP would trap RNAPs and thus improve the expression of PuuC, an aldehyde dehydrogenase catalyzing 3-hydroxypropionaldehyde (3-HPA) to 3-hydroxypropionic acid (3-HP) in Klebsiella pneumoniae...
January 16, 2018: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29334043/a-tribute-to-james-parkinson
#16
André Parent
Exactly 200 years ago, the London surgeon-apothecary James Parkinson (1755-1824) published a 66-page-long booklet entitled An Essay on the Shaking Palsy, which contains the first clear clinical description of the shaking palsy or paralysis agitans, which we now refer to as Parkinson's disease. However, the value of this essay was not fully recognized during Parkinson's lifetime, which spanned the American Revolution, the French Revolution, and the Napoleonic Wars. James Parkinson was one of the most singular figures of his time and place...
January 2018: Canadian Journal of Neurological Sciences. le Journal Canadien des Sciences Neurologiques
https://www.readbyqxmd.com/read/29332008/leg-stereotypy-syndrome-phenomenology-and-prevalence
#17
Mitesh Lotia, Michele K York, Adriana M Strutt, Joseph Jankovic
OBJECTIVES: To describe the phenomenology and prevalence of leg stereotypy syndrome (LSS), characterised chiefly by repetitive, rhythmical, stereotypic leg movement, especially when sitting. METHODS: We sought to characterise LSS in two groups of subjects: (1) general population (GP) group, defined as individuals accompanying patients during their visits to Baylor College of Medicine Parkinson's Disease Center and Movement Disorders Clinic who are not genetically related to the patients; and (2) movement disorders (MD) group, composed of consecutive patients with diagnoses of restless legs syndrome, Parkinson's disease, Tourette syndrome and tardive dyskinesia...
January 13, 2018: Journal of Neurology, Neurosurgery, and Psychiatry
https://www.readbyqxmd.com/read/29332000/how-good-are-doctors-at-introducing-themselves-hellomynameis
#18
Peter Gillen, Sue Faye Sharifuddin, Muireann O'Sullivan, Alison Gordon, Eva M Doherty
BACKGROUND: This explorative study was triggered by the '#hellomynameis' campaign initiated by Dr Kate Granger in the UK. Our objectives were twofold: first, to measure rates of introduction in an Irish hospital setting by both consultant and non-consultant hospital doctors. Second to establish whether such practices were associated with patient perceptions of the doctor/patient interaction. METHOD: A patient 'exit' survey was undertaken following doctor-patient consultations in both acute (surgical and medical assessment units) and elective settings (outpatient clinics)...
January 13, 2018: Postgraduate Medical Journal
https://www.readbyqxmd.com/read/29331528/expression-of-a-thermotolerant-laccase-from-pycnoporus-sanguineus-in-trichoderma-reesei-and-its-application-in-the-degradation-of-bisphenol-a
#19
Jie Zhao, Shenquan Zeng, Ying Xia, Liming Xia
The laccase gene from Pycnoporus sanguineus was cloned and inserted between the strong Pcbh1 promoter and the Tcbh1 terminator from Trichoderma reesei to form the recombinant plasmid pCH-lac. Using Agrobacterium-mediated technique, the pCH-lac was integrated into the chromosomes of T. reesei. Twenty positive transformants were obtained by employing hygromycin B as a selective agent. PCR was used to confirm that the laccase gene was integrated into the chromosomal DNA of T. reesei. Laccase production by recombinant transformants was performed in shaking flasks, and the activity of laccase reached 8...
January 10, 2018: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/29330738/a-video-review-of-multiple-concussion-signs-in-national-rugby-league-match-play
#20
Andrew J Gardner, David R Howell, Grant L Iverson
BACKGROUND: Video review has been introduced in many professional sports worldwide to help recognize concussions. However, to date, there has been very little research on the accuracy of using video analysis to identify signs of concussion and the various combinations of observed signs. METHODS: The objective of the study is to determine the accuracy of combinations of clinical signs of concussion identified using video analysis to identify concussions in the National Rugby League (NRL)...
January 12, 2018: Sports Medicine—Open
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