keyword
MENU ▼
Read by QxMD icon Read
search

Shaking

keyword
https://www.readbyqxmd.com/read/28344901/can-video-playback-provide-social-information-for-foraging-blue-tits
#1
Liisa Hämäläinen, Hannah M Rowland, Johanna Mappes, Rose Thorogood
Video playback is becoming a common method for manipulating social stimuli in experiments. Parid tits are one of the most commonly studied groups of wild birds. However, it is not yet clear if tits respond to video playback or how their behavioural responses should be measured. Behaviours may also differ depending on what they observe demonstrators encountering. Here we present blue tits (Cyanistes caeruleus) videos of demonstrators discovering palatable or aversive prey (injected with bitter-tasting Bitrex) from coloured feeding cups...
2017: PeerJ
https://www.readbyqxmd.com/read/28344744/automation-of-pacbio-smrtbell-ngs-library-preparation-for-bacterial-genome-sequencing
#2
Nguyet Kong, Whitney Ng, Kao Thao, Regina Agulto, Allison Weis, Kristi Spittle Kim, Jonas Korlach, Luke Hickey, Lenore Kelly, Stephen Lappin, Bart C Weimer
BACKGROUND: The PacBio RS II provides for single molecule, real-time DNA technology to sequence genomes and detect DNA modifications. The starting point for high-quality sequence production is high molecular weight genomic DNA. To automate the library preparation process, there must be high-throughput methods in place to assess the genomic DNA, to ensure the size and amounts of the sheared DNA fragments and final library. FINDINGS: The library construction automation was accomplished using the Agilent NGS workstation with Bravo accessories for heating, shaking, cooling, and magnetic bead manipulations for template purification...
2017: Standards in Genomic Sciences
https://www.readbyqxmd.com/read/28344389/reductive-activity-of-free-and-immobilized-cells-of-cyanobacteria-toward-oxophosphonates-comparative-study
#3
Monika Górak, Ewa Żymańczyk-Duda
This report, based on the previous studies, compares the reductive activity of different modes of following photobiocatalysts (on laboratory and preparative scale): Arthrospira maxima, Nostoc cf. muscorum and Nodularia sphaerocarpa, toward diethyl esters of 2-oxopropylphosphonate (1), 2-oxo-2-phenylethylphosphonate (2), and 2-oxobutylphosphonate (3). It was confirmed that immobilization in alginate matrix do not affect the activity and viability of the biocatalysts. Corresponding (S)-hydroxyphosphonates (1a-3a) were obtained with similar efficiency compared to the free-cell mode with the yield and of the optical purity e...
2017: Journal of Applied Phycology
https://www.readbyqxmd.com/read/28343939/a-tale-of-gastric-layering-and-sieving-gastric-emptying-of-a-liquid-meal-with-water-blended-in-or-consumed-separately
#4
Guido Camps, Monica Mars, Cees de Graaf, Paul A M Smeets
BACKGROUND: The process of gastric emptying determines how fast gastric content is delivered to the small intestine. It has been shown that solids empty slower than liquids and that a blended soup empties slower than the same soup as broth and chunks, due to the liquid fraction emptying more quickly. This process of 'gastric sieving' has not been investigated for liquid foods. OBJECTIVE: To determine whether gastric sieving of water can also occur for liquid foods...
March 23, 2017: Physiology & Behavior
https://www.readbyqxmd.com/read/28343780/propagation-of-brazilian-zika-virus-strains-in-static-and-suspension-cultures-using-vero-and-bhk-cells
#5
Alexander Nikolay, Leda R Castilho, Udo Reichl, Yvonne Genzel
The recent spread of Zika virus (ZIKV) in the Americas and the Pacific has reached alarming levels in more than 60 countries. However, relatively little is known about the disease on a virological and epidemiological level and its consequences for humans. Accordingly, a large demand for in vitro derived Brazilian ZIKV material to support in vitro and in vivo studies has arisen. However, a prompt supply of ZIKV and ZIKV antigens cannot be guaranteed as the production of this virus typically using Vero or C6/36 cell lines remains challenging...
March 23, 2017: Vaccine
https://www.readbyqxmd.com/read/28343071/analgesia-effect-of-baicalein-against-ntg-induced-migraine-in-rats
#6
Xiao-Fan Zhang, Wen-Jun Zhang, Cui-Lan Dong, Wan-Li Hu, Yu-Yao Sun, Yarigui Bao, Chun-Feng Zhang, Chang-Run Guo, Chong-Zhi Wang, Chun-Su Yuan
BACKGROUND: Migraine is a complex nervous system disease characterized by typical throbbing and unilateral headache, which causes severe healthy and social issues worldwide. The purpose of this study was to investigate the effect of baicalein (BAI) on the treatment of migraine. MATERIAL AND METHODS: Twenty-four rats were randomly divided equally into four groups, including a blank group, model group, positive group (ibuprofen tablets 82mg/kg), and BAI group (60mg/kg)...
March 23, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28342964/establishing-a-novel-biosynthetic-pathway-for-the-production-of-3-4-dihydroxybutyric-acid-from-xylose-in-escherichia-coli
#7
Jia Wang, Xiaolin Shen, Rachit Jain, Jian Wang, Qipeng Yuan, Yajun Yan
3-Hydroxy-γ-butyrolactone (3HBL) is an attractive building block owing to its broad applications in pharmaceutical industry. Currently, 3HBL is commercially produced by chemical routes using petro-derived carbohydrates, which involves hazardous materials and harsh processing conditions. Only one biosynthetic pathway has been reported for synthesis of 3HBL and its hydrolyzed form 3,4-dihydroxybutyric acid (3,4-DHBA) using glucose and glycolic acid as the substrates and coenzyme A as the activator, which involves multiple steps (>10 steps) and suffers from low productivity and yield...
March 23, 2017: Metabolic Engineering
https://www.readbyqxmd.com/read/28342835/lipids-in-exosomes-current-knowledge-and-the-way-forward
#8
REVIEW
Tore Skotland, Kirsten Sandvig, Alicia Llorente
Lipids are essential components of exosomal membranes, and it is well-known that specific lipids are enriched in exosomes compared to their parent cells. In this review we discuss current knowledge about the lipid composition of exosomes. We compare published data for different lipid classes in exosomes, and what is known about their lipid species, i.e. lipid molecules with different fatty acyl groups. Moreover, we elaborate on the hypothesis about hand-shaking between the very-long-chain sphingolipids in the outer leaflet and PS 18:0/18:1 in the inner leaflet, and we propose this to be an important mechanism in membrane biology, not only for exosomes...
March 23, 2017: Progress in Lipid Research
https://www.readbyqxmd.com/read/28341625/non-cardiac-surgery-in-patients-with-continuous-flow-left-ventricular-assist-devices-a-single-institutional-experience
#9
Carol W Chen, Kristoffel R Dumon, Oren Shaked, Michael A Acker, Pavan Atluri, Daniel T Dempsey
With improvements in life expectancy for patients with continuous-flow left ventricular assist devices (LVADs), non-cardiac surgeons will increasingly encounter surgical problems in this population. 209 patients underwent LVAD placement between 10/1/2007 and 6/1/2015 at a single institution. Survival was compared between patients who had non-cardiac surgery (NCS) during the initial LVAD implantation hospitalization (n=36) and those who had NCS only in subsequent hospitalizations (n=33). Postoperative complication rates were examined...
March 24, 2017: Journal of Investigative Medicine: the Official Publication of the American Federation for Clinical Research
https://www.readbyqxmd.com/read/28339377/stability-of-clindamycin-hydrochloride-in-pcca-base-suspendit
#10
Yashoda V Pramar, Richard A Graves, Grace A Ledet, Kelly V Phan, Levon A Bostanian, Tarun K Mandal
Clindamycin is an effective antibiotic in the treatment of infections caused by certain gram-positive and gram-negative anaerobic microorganisms. While manufactured forms of the drug for pediatric use are available, there are instances when a compounded liquid dosage form is essential to meet unique patient needs. The purpose of this study was to determine the chemical stability of clindamycin hydrochloride in the PCCA base SuspendIt, a sugar-free, paraben- free, dye-free, and gluten-free thixotropic vehicle containing a natural sweetener obtained from the monk fruit...
September 2016: International Journal of Pharmaceutical Compounding
https://www.readbyqxmd.com/read/28336989/meeting-and-greeting-in-the-clinical-setting-are-we-doing-what-patients-want
#11
A Davies-House, N Ball, C Balmer
Objective To determine how patients want to be greeted by clinicians on a first encounter in the clinical setting.Setting A UK dental teaching hospital in 2015/16.Materials and methods Data was collected prospectively via 450 patient questionnaires. The results were stratified by generational cohort and compared to assess if there was an association between patient preferences and the generational theory.Results Patients preferred to be greeted informally by their first name and didn't mind how the clinician introduced themselves or preferred them to use their first name also...
March 24, 2017: British Dental Journal
https://www.readbyqxmd.com/read/28335028/defining-the-5%C3%AE-and-3%C3%AE-landscape-of-the-drosophila-transcriptome-with-exo-seq-and-rnaseh-seq
#12
Shaked Afik, Osnat Bartok, Maxim N Artyomov, Alexander A Shishkin, Sabah Kadri, Mor Hanan, Xiaopeng Zhu, Manuel Garber, Sebastian Kadener
Cells regulate biological responses in part through changes in transcription start sites (TSS) or cleavage and polyadenylation sites (PAS). To fully understand gene regulatory networks, it is therefore critical to accurately annotate cell type-specific TSS and PAS. Here we present a simple and straightforward approach for genome-wide annotation of 5΄- and 3΄-RNA ends. Our approach reliably discerns bona fide PAS from false PAS that arise due to internal poly(A) tracts, a common problem with current PAS annotation methods...
February 22, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28331815/measuring-e-coli-and-bacteriophage-dna-in-cell-sonicates-to-evaluate-the-cal1-reaction-as-a-synthetic-biology-standard-for-qpcr
#13
Alexander Templar, Desmond M Schofield, Darren N Nesbeth
We measured the impact of the presence of total Escherichia coli (E. coli) cellular material on the performance of the Linear Regression of Efficiency (LRE) method of absolute quantitative PCR (LRE qPCR), which features the putatively universal CAL1 calibration reaction, which we propose as a synthetic biology standard. We firstly used a qPCR reaction in which a sequence present in the lone genomic BirA locus is amplified. Amplification efficiency for this reaction, a key metric for many quantitative qPCR methods, was inhibited by cellular material from bioreactor cultivation to a greater extent than material from shake flask cultivation...
March 2017: Biomolecular Detection and Quantification
https://www.readbyqxmd.com/read/28330755/microwave-assisted-modification-of-cellulose-by-gallic-acid-and-its-application-for-removal-of-aluminium-from-real-samples
#14
W I Mortada, I M M Kenawy, Y G Abou El-Reash, A A Mousa
Microwave assisted preparation of cellulose modified with Gallic acid (MA-Cell-GA) was developed for high efficient adsorption of Al(3+). The as-prepared modified cellulose has been characterized by elemental analysis, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), transmission electron microscopy (TEM) and zeta potential measurements. Energy dispersive X-ray (EDX) spectrum was utilized to proof the adsorption of Al(3+). The effect of various experimental factors, as pH, amount of adsorbent, shaking time, initial metal ion concentration, temperature, concomitant ions and desorption conditions on the extraction efficiency was investigated and optimized in batch mode experiments...
March 19, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28330311/scaling-up-a-virginiamycin-production-by-a-high-yield-streptomyces-virginiae-vkm-ac-2738d-strain-using-adsorbing-resin-addition-and-fed-batch-fermentation-under-controlled-conditions
#15
Vakhtang Dzhavakhiya, Vyacheslav Savushkin, Alexander Ovchinnikov, Vladislav Glagolev, Veronika Savelyeva, Evgeniya Popova, Nikita Novak, Elena Glagoleva
Virginiamycin produced by Streptomyces virginiae as a natural mix of macrocyclic peptidolactones M and S is widely used in the industrial production of ethanol fuel and as an antibiotic feed additive for cattle and poultry. Its main antimicrobial components, M1 and S1 factors, act synergistically if the M1:S1 ratio in the final product is 70-75:25-30. This fact significantly complicates the development of stable high-yield strains suitable for industrial application. In the previous work, authors obtained a mutant S...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330255/ascorbyl-palmitate-synthesis-in-an-organic-solvent-system-using-a-celite-immobilized-commercial-lipase-lipolase-100l
#16
Shivika Sharma, Kriti Kanwar, Shamsher S Kanwar
Ascorbyl palmitate was synthesized using a Celite-immobilized commercial lipase (Lipolase 100L) in dimethylsulfoxide (DMSO) as an organic solvent system. Lipase immobilized by surface adsorption onto Celite 545 matrix and subsequently exposed to 1 % glutaraldehyde showed 75 % binding of protein. The Celite-bound lipase was optimally active at 75 °C and pH 8.5 under shaking and showed maximum hydrolytic activity toward p-NPP as a substrate. The bound lipase was found to be stimulated only in the presence of Al(3+) and EDTA...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330234/optimization-of-cellulase-production-by-penicillium-sp
#17
H N Prasanna, G Ramanjaneyulu, B Rajasekhar Reddy
The production of cellulolytic enzymes (β-exoglucanase, β-endoglucanase and β-glucosidase) by Penicillium sp. on three different media in liquid shake culture conditions was compared. The organism exhibited relatively highest activity of endoglucanase among three enzymes measured at 7-day interval during the course of its growth on Czapek-Dox medium supplemented with 0.5 % (w/v) cellulose. Cellulose at 0.5 %, lactose at 0.5 %, sawdust at 0.5 %, yeast extract at 0.2 % as a nitrogen source, pH 5.0 and 30 °C temperature were found to be optimal for growth and cellulase production by Penicillium sp...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330227/statistical-modeling-and-optimization-of-culture-conditions-by-response-surface-methodology-for-2-4-and-2-6-dinitrotoluene-biodegradation-using-rhodococcus-pyridinivorans-nt2
#18
Debasree Kundu, Chinmay Hazra, Ambalal Chaudhari
To improve biodegradability (% biodegradation) and specific growth rate of Rhodococcus pyridinivorans NT2, culture medium and environmental parameters were screened and optimized using the statistical design techniques of Plackett-Burman and response surface methodology. Of the process variables screened, DNTs (2,4-DNT and 2,6-DNT), MgSO4·7H2O, temperature and inoculum size (O.D.) were selected as the most important (P value <0.05) factors. In multiresponse analysis of central composite design, medium formulation consisting of 474/470 mg l(-1) 2,4-DNT/2,6-DNT, 0...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330214/production-kinetics-of-polyhydroxyalkanoates-by-using-pseudomonas-aeruginosa-gamma-ray-mutant-strain-ebn-8-cultured-on-soybean-oil
#19
Sharjeel Abid, Zulfiqar Ali Raza, Tanveer Hussain
The purpose of present study was to optimize polyhydroxyalkanotes (PHAs) production in a gamma ray mutant strain of Pseudomonas aeruginosa grown on soybean oil in minimal salts media under shake flask conditions. The production kinetics was studied by sampling on daily basis for 6 days to investigate the best conditions for PHAs production like biomass estimation, carbon source utilization and PHAs yield. The PHA accumulation was observed up to 50.27 % (w/w) of cell dry mass. The Pseudomonas species synthesized medium chain length PHA copolyester as per identified by LCMS and confirmed by FTIR spectroscopy...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330140/statistical-based-optimization-and-scale-up-of-siderophore-production-process-on-laboratory-bioreactor
#20
S S Shaikh, S J Wani, R Z Sayyed
We report the enhanced production of siderophore in succinate medium by applying two-stage statistical approach, i.e., Plackett-Burman design and response surface methodology (RSM) using central composite design (CCD). In the first stage of optimization, out of 11 variable components of succinate medium, succinic acid, pH and temperature were found as significant components that influenced the siderophore production in Pseudomonas aeruginosa RZS9. The second stage of RSM using CCD consisted of optimizing the concentrations of the variables...
June 2016: 3 Biotech
keyword
keyword
13664
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"