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Enhanced recovery

Andrea Becerra-Calixto, Rafael Posada-Duque, Gloria Patricia Cardona-Gómez
Astrocytes play metabolic and structural support roles and contribute to the integrity of the blood-brain barrier (BBB), linking communication between neurons and the endothelium. Cyclin-dependent kinase 5 (CDK5) likely exerts a dual effect on the endothelium and astrocytes due to its involvement in migration and angiogenesis; the overactivation of CDK5 is associated with dysfunction in glutamate recapture and hypoxia. Recently, we proposed that CDK5-targeted astrocytes facilitate the recovery of neurological and motor function in transplanted ischemic rats...
March 22, 2018: Molecular Neurobiology
Clint L Makino, Teresa Duda, Alexandre Pertzev, Rameshwar K Sharma
Our ability to see is based on the activity of retinal rod and cone photoreceptors. Rods function when there is very little light, while cones operate at higher light levels. Photon absorption by rhodopsin activates a biochemical cascade that converts photic energy into a change in the membrane potential of the cell by decreasing the levels of a second messenger, cGMP, that control the gating of cation channels. But just as important as the activation of the cascade are the shut-off and recovery processes. The timing of shutoff and recovery ultimately affects sensitivity, temporal resolution and even the capacity for counting single photons...
2018: Methods in Molecular Biology
Soheil Saedi, Sayed E Saghaian, Ahmadreza Jahadakbar, Narges Shayesteh Moghaddam, Mohsen Taheri Andani, Sayed M Saghaian, Y Charles Lu, Mohammad Elahinia, Haluk E Karaca
Porous NiTi scaffolds display unique bone-like properties including low stiffness and superelastic behavior which makes them promising for biomedical applications. The present article focuses on the techniques to enhance superelasticity of porous NiTi structures. Selective Laser Melting (SLM) method was employed to fabricate the dense and porous (32-58%) NiTi parts. The fabricated samples were subsequently heat-treated (solution annealing + aging at 350 °C for 15 min) and their thermo-mechanical properties were determined as functions of temperature and stress...
March 21, 2018: Journal of Materials Science. Materials in Medicine
Valentina A Babenko, Denis N Silachev, Vasily A Popkov, Ljubava D Zorova, Irina B Pevzner, Egor Y Plotnikov, Gennady T Sukhikh, Dmitry B Zorov
A recently discovered key role of reactive oxygen species (ROS) in mitochondrial traffic has opened a wide alley for studying the interactions between cells, including stem cells. Since its discovery in 2006, intercellular mitochondria transport has been intensively studied in different cellular models as a basis for cell therapy, since the potential of replacing malfunctioning organelles appears to be very promising. In this study, we explored the transfer of mitochondria from multipotent mesenchymal stem cells (MMSC) to neural cells and analyzed its efficacy under normal conditions and upon induction of mitochondrial damage...
March 19, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Michael P Kilgard, Robert L Rennaker, Jen Alexander, Jesse Dawson
BACKGROUND: Recent studies indicate that vagus nerve stimulation (VNS) paired with rehabilitation can enhance neural plasticity in the primary sensory and motor cortices, improve forelimb function after stroke in animal models and improve motor function in patients with arm weakness after stroke. OBJECTIVE: To gain "first-in-man" experience of VNS paired with tactile training in a patient with severe sensory impairment after stroke. METHODS: During the long-term follow-up phase of a clinical trial of VNS paired with motor rehabilitation, a 71-year-old man who had made good motor recovery had ongoing severe sensory loss in his left hand and arm...
2018: NeuroRehabilitation
Nuket Kartal Temel, Ramazan Gürkan
A novel ultrasound assisted-cloud point extraction method was developed for preconcentration and determination of V(V) in beverage samples. After complexation by pyrogallol in presence of safranin T at pH 6.0, V(V) ions as ternary complex are extracted into the micellar phase of Triton X-114. The complex was monitored at 533 nm by spectrophotometry. The matrix effect on the recovery of V(V) from the spiked samples at 50 μg L-1 was evaluated. In optimized conditions, the limits of detection and quantification of the method, respectively, was 0...
March 2018: Acta Chimica Slovenica
Li Guo, Ya-Wei Hao, Pei-Long Li, Jiang-Feng Song, Rui-Zhu Yang, Xiu-Yan Fu, Sheng-Yi Xie, Jing Zhao, Yong-Lai Zhang
We report on the fabrication of a NO2 gas sensor from room-temperature reduction of graphene oxide(GO) via two-beam-laser interference (TBLI). The method of TBLI gives the distribution of periodic dissociation energies for oxygen functional groups, which are capable to reduce the graphene oxide to hierarchical graphene nanostructures, which holds great promise for gaseous molecular adsorption. The fabricated reduced graphene oxide(RGO) sensor enhanced sensing response in NO2 and accelerated response/recovery rates...
March 20, 2018: Scientific Reports
Odile Francesca Restaino, Maria Giovanna Borzacchiello, Ilaria Scognamiglio, Luigi Fedele, Alberto Alfano, Elena Porzio, Giuseppe Manco, Mario De Rosa, Chiara Schiraldi
BACKGROUND: Thermostable phosphotriesterase-like lactonases (PLLs) are able to degrade organophosphates and could be potentially employed as bioremediation tools and bioscavengers. But nowadays their manufacturing in high yields is still an issue that limits their industrial applications. In this work we aimed to set up a high yield production and purification biotechnological process of two recombinant PLLs expressed in E. coli, the wild type SacPox from Sulfolobus acidocaldarius and a triple mutated SsoPox C258L/I261F/W263A, originally from Sulfolobus solfataricus...
March 20, 2018: BMC Biotechnology
Khushboo Patel, Basant Roondhe, Shweta D Dabhi, Prafulla K Jha
Recently predicted and grown new single element two dimensional (2D) material borophene gathered tremendous research interest due to its structural, electronic and other properties. Using first principles based dispersion corrected density functional calculations, we have studied interaction of two toxic gases phosgene (COCl2 ) and carbon monoxide (CO) with borophene to understand the role of borophene as biosensor and carriers in drug delivery. The sensing behaviour of borophene towards COCl2 and CO has been studied by calculating the binding energy and electronic density of states (DOS)...
March 7, 2018: Journal of Hazardous Materials
Yanyan Gao, Jianping Xu, Shaobo Shi, Hong Dong, Yahui Cheng, Chengtai Wei, Xiaosong Zhang, Shougen Yin, Lan Li
The self-powered ultraviolet photodetectors (UV PDs) have attracted increasing attention due to their potential applications without consuming any external power. It is important to obtain the high performance self-powered UV PDs by a simple method for the practical application. Herein, TiO2 nanorod arrays (NRs) were synthesized by hydrothermal method, which were integrated with p-type NiO nanoflakes to realize a high performance pn heterojunction for the efficient UV photodetection. TiOx thin film can improve the morphological and carrier transport properties of TiO2 NRs, and decrease the surface and defect states, resulting in the enhanced photocurrent of the devices...
March 20, 2018: ACS Applied Materials & Interfaces
Jing Li, Shuwen Xue, Chunqiu He, Huixia Qi, Fulin Chen, Yanling Ma
Pseudomonas aeruginosa DN1 strain and Bacillus subtilis QHQ110 strain were chosen as rhamnolipid and lipopeptide producer respectively, to evaluate the efficiency of exogenous inoculants on enhancing oil recovery (EOR) and to explore the relationship between injected bacteria and indigenous bacterial community dynamics in long-term filed pilot of Hujianshan low permeability water-flooded reservoir for 26 months. Core-flooding tests showed that the oil displacement efficiency increased by 18.46% with addition of exogenous consortia...
March 20, 2018: World Journal of Microbiology & Biotechnology
Maziar Derakhshandeh, Brandy K Pilapil, Ben Workman, Milana Trifkovic, Steven L Bryant
Emulsions are widely used in industrial applications, including in food sciences, cosmetics, and enhanced oil recovery. For these industries, an in depth understanding of the stability and rheological properties of emulsions under both static and dynamic conditions is vital to their successful application. Presented here is a thorough assessment of a model nanoparticle (NP) stabilized dodecane-in-water emulsion as a route to improved understanding of the relationship between NP properties, microstructure and droplet-droplet interactions on the stability and rheological properties of emulsions...
March 20, 2018: Soft Matter
Janet Baack Kukreja, Qiuling Shi, Courtney M Chang, Mohamed A Seif, Brandon M Sterling, Ting-Yu Chen, Kelly M Creel, Ashish M Kamat, Colin P Dinney, Neema Navai, Jay B Shah, Xin Shelley Wang
BACKGROUND: Bladder cancer is a disease of the elderly that is associated with high morbidity in those treated with radical cystectomy. In this observational study of patients with bladder cancer undergoing radical cystectomy, we analyzed and compared patient-reported outcomes from those treated with Enhanced Recovery After Surgery (ERAS) methods versus those who received traditional perioperative care. METHODS: We enrolled patients who underwent radical cystectomy at a high-volume tertiary care referral center from November 2013 to December 2016, when the ERAS concept was being introduced into postoperative care at our institution...
March 1, 2018: Surgical Innovation
Xiu-Xiu Zhang, Yi-Zhen Song, Fang Fang, Zhi-Yong Wu
On-site rapid monitoring of nitrite as an assessment indicator of the environment, food, and physiological systems has drawn extensive attention. Here, electrokinetic stacking (ES) was combined with colorimetric reaction on a paper-based device (PAD) to achieve colorless nitrite detection with smartphone. In this paper, nitrite was stacked on the paper fluidic channel as a narrow band by electrokinetic stacking. Then, Griess reagent was introduced to visualize the stacking band. Under optimal conditions, the sensitivity of nitrite was 160-fold increased within 5 min...
March 19, 2018: Analytical and Bioanalytical Chemistry
Anniina Vihervaara, Fabiana M Duarte, John T Lis
Proteotoxic stress, that is, stress caused by protein misfolding and aggregation, triggers the rapid and global reprogramming of transcription at genes and enhancers. Genome-wide assays that track transcriptionally engaged RNA polymerase II (Pol II) at nucleotide resolution have provided key insights into the underlying molecular mechanisms that regulate transcriptional responses to stress. In addition, recent kinetic analyses of transcriptional control under heat stress have shown how cells 'prewire' and rapidly execute genome-wide changes in transcription while concurrently becoming poised for recovery...
March 19, 2018: Nature Reviews. Genetics
Adam B King, Matthew D Spann, Patrick Jablonski, Jonathan P Wanderer, Warren S Sandberg, Matthew D McEvoy
BACKGROUND: Patients frequently remain in the hospital after bariatric surgery due to pain, nausea, and inability to tolerate oral intake. Enhanced recovery after surgery (ERAS) concepts address these perioperative complications and therefore improve length of stay for bariatric surgery patients. OBJECTIVES: To determine if ERAS concepts increase the proportion of patients discharged on postoperative day 1. Secondary objectives included mean length of stay, perioperative opioid use, emergency department visits, and readmissions...
February 13, 2018: Surgery for Obesity and related Diseases: Official Journal of the American Society for Bariatric Surgery
Yosuke Tomita, Aditi A Mullick, Mindy F Levin
Kinematic redundancy of the human body provides abundant movement patterns to accomplish the same motor goals (motor equivalence). Compensatory movement patterns such as excessive trunk displacement in stroke subjects during reaching can be viewed as a consequence of the motor equivalent process to accomplish a task despite limited available ranges in some joints. However, despite compensations, the ability to adapt reaching performance when perturbations occur may still be limited when condition-specific changes of joint angles are required...
February 2018: Neurorehabilitation and Neural Repair
Ziqing Kong, Shaodong Jia, Anna Lena Chabes, Patrik Appelblad, Richard Lundmark, Thomas Moritz, Andrei Chabes
Information about the intracellular concentration of dNTPs and NTPs is important for studies of the mechanisms of DNA replication and repair, but the low concentration of dNTPs and their chemical similarity to NTPs present a challenge for their measurement. Here, we describe a new rapid and sensitive method utilizing hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry for the simultaneous determination of dNTPs and NTPs in biological samples. The developed method showed linearity (R2 > 0...
March 15, 2018: Nucleic Acids Research
Zhen Ma, Huisheng Zhuang
Benzo[a]pyrene (BaP), a type of polycyclic aromatic hydrocarbon, is widely distributed in the environment. In this study, BaP immunogen and coating antigen were respectively prepared by different methods, and the specific antibody targeting the BaP analyte was obtained. Moreover, gold nanoparticles modified with the specific polyclonal antibody and thiol-capped DNA were prepared as biological probes. Based on the work above, an ultrasensitive biological probe enhanced real-time immuno-polymerase chain reaction (BP-rt-iPCR) assay was developed to detect BaP in several environmental samples...
March 15, 2018: Acta Biochimica et Biophysica Sinica
Jonathan T Ting, Brian R Lee, Peter Chong, Gilberto Soler-Llavina, Charles Cobbs, Christof Koch, Hongkui Zeng, Ed Lein
This protocol is a practical guide to the N-methyl-D-glucamine (NMDG) protective recovery method of brain slice preparation. Numerous recent studies have validated the utility of this method for enhancing neuronal preservation and overall brain slice viability. The implementation of this technique by early adopters has facilitated detailed investigations into brain function using diverse experimental applications and spanning a wide range of animal ages, brain regions, and cell types. Steps are outlined for carrying out the protective recovery brain slice technique using an optimized NMDG artificial cerebrospinal fluid (aCSF) media formulation and enhanced procedure to reliably obtain healthy brain slices for patch clamp electrophysiology...
February 26, 2018: Journal of Visualized Experiments: JoVE
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