Marie-Charlotte Meinsohn, Hatice D Saatcioglu, Lina Wei, Yi Li, Heiko Horn, Maeva Chauvin, Motohiro Kano, Ngoc Minh Phuong Nguyen, Nicholas Nagykery, Aki Kashiwagi, Wesley R Samore, Dan Wang, Esther Oliva, Guangping Gao, Mary E Morris, Patricia K Donahoe, David Pépin
Müllerian inhibiting substance (MIS/AMH), produced by granulosa cells of growing follicles, is an important regulator of folliculogenesis and follicle development. Treatment with exogenous MIS in mice suppresses follicle development and prevents ovulation. To investigate the mechanisms by which MIS inhibits follicle development, we performed single-cell RNA sequencing of whole neonatal ovaries treated with MIS at birth and analyzed at postnatal day 6, coinciding with the first wave of follicle growth. We identified distinct transcriptional signatures associated with MIS responses in the ovarian cell types...
May 18, 2021: Proceedings of the National Academy of Sciences of the United States of America