keyword
MENU ▼
Read by QxMD icon Read
search

Candida molecular typing

keyword
https://www.readbyqxmd.com/read/27905002/disseminated-candidiasis-in-a-young-previously-healthy-dog-and-review-of-literature
#1
Nicole Willems, Dirk J Houwers, Yvette M Schlotter, Bart Theelen, Teun Boekhout
BACKGROUND: The reports on disseminated candidiasis in dogs so far describe at least one predisposing factor. This case report, however, highlights candidiasis in a dog without any known predisposition. PATIENT: A 1.5-year-old intact female Hovawart dog was presented with subcutaneous nodules and polyuria/polydipsia. An excisional biopsy revealed a chronic pyogranulomatous and necrotizing inflammation with mycotic structures. The patient became febrile and lethargic, and developed lameness...
November 30, 2016: Mycopathologia
https://www.readbyqxmd.com/read/27882277/effect-of-silver-nanoparticles-on-the-standard-soil-arthropod-folsomia-candida-collembola-and-the-eukaryote-model-organism-saccharomyces-cerevisiae
#2
Panwad Sillapawattana, Martin C H Gruhlke, Andreas Schäffer
BACKGROUND: Because of their antimicrobial properties, silver nanoparticles (AgNPs) have been widely used and have come into contact with the environment. In the present work, an effect of AgNPs on a standard soil organism, Folsomia candida, was studied (in comparison to silver nitrate) focusing on molecular and cellular alterations as ecotoxicological endpoints. RESULTS: At the molecular level, an up-regulation of metallothionein-containing protein (MTC) mRNA in AgNP-treated groups indicated toxic heavy metal stress effects caused by the release of silver ions from AgNPs, which is similar to animal groups treated with silver nitrate...
2016: Environmental Sciences Europe
https://www.readbyqxmd.com/read/27837497/the-changing-epidemiology-of-invasive-fungal-infections
#3
David A Enoch, Huina Yang, Sani H Aliyu, Christianne Micallef
Invasive fungal infections (IFI) are an emerging problem worldwide with invasive candidiasis and candidemia responsible for the majority of cases. This is predominantly driven by the widespread adoption of aggressive immunosuppressive therapy among certain patient populations (e.g., chemotherapy, transplants) and the increasing use of invasive devices such as central venous catheters (CVCs). The use of new immune modifying drugs has also opened up an entirely new spectrum of patients at risk of IFIs. While the epidemiology of candida infections has changed in the last decade, with a gradual shift from C...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27836850/elimination-of-a-free-cysteine-by-creation-of-a-disulfide-bond-increases-the-activity-and-stability-of-candida-boidinii-formate-dehydrogenase
#4
Junxian Zheng, Taowei Yang, Junping Zhou, Meijuan Xu, Xian Zhang, Zhiming Rao
: NAD(+)-dependent formate dehydrogenase (FDH, EC 1.2.1.2) is an industrial enzyme widely used for NADH regeneration. However, enzyme inactivation caused by the oxidation of cysteine residues is a flaw of native FDH. In this study, we relieved the oxidation of the free cysteine of FDH from Candida boidinii (CboFDH) through the construction of disulfide bonds between A10 and C23 as well as I239 and C262. Variants A10C, I239C and A10C/I239C were obtained by the site-directed mutagenesis and their properties were studied...
November 11, 2016: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/27816903/molecular-and-antifungal-susceptibility-study-on-trichosporonemia-and-emergence-of-trichosporon-mycotoxinivorans-as-a-bloodstream-pathogen
#5
Yubhisha Dabas, Immaculata Xess, Pratibha Kale
A total of 21 Trichosporon spp. isolates from blood over a period of 5 years (January 2009 to December 2013) were included in the study. The most common underlying diseases found were pancreatitis (33.3%) and cancer (33.3%). Trichosporon asahii (80.9%) was the commonest species followed by Trichosporon mycotoxinivorans (14.2%) and Trichosporon faecale (4.7%). On IGS1 region sequencing the most predominant T. asahii type in our region was genotype 1 (16/17 isolates; 94.1%) and one isolate belonged to genotype 4...
November 5, 2016: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
https://www.readbyqxmd.com/read/27744307/genetic-diversity-of-the-hwp1-gene-and-his3-ef3-cdc3-microsatellites-and-antifungal-susceptibility-profiles-of-candida-albicans-isolates-from-yaound%C3%A3-hiv-infected-patients
#6
Thierry Kammalac Ngouana, Pascal Drakulovski, Donika Krasteva, Rufin Kuipou Toghueo, Charles Kouanfack, Jacques Reynes, Eric Delaporte, Fabrice Fekam Boyom, Michèle Mallié, Sébastien Bertout
The molecular epidemiology and the antifungal susceptibility profiles of Candida albicans are scarce in Cameroon. Authors studied the genetic diversity and the antifungal susceptibility of C. albicans isolates from Yaoundé HIV-infected patients. Clinical isolates were obtained by mycological diagnosis of oropharyngeal swabs, stools, urine, and vaginal swabs from patients. C. albicans isolates were confirmed by the Light cycler real-time PCR of the ITS1 region of the 5.8s ribosomal DNA. The ABC genotypes and the Hwp1 gene amplification were carried out with specific primers...
October 14, 2016: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
https://www.readbyqxmd.com/read/27727302/candida-albicans-isw2-regulates-chlamydospore-suspensor-cell-formation-and-virulence-in-vivo-in-a-mouse-model-of-disseminated-candidiasis
#7
Dhammika H M L P Navarathna, Ruvini U Pathirana, Michail S Lionakis, Kenneth W Nickerson, David D Roberts
Formation of chlamydospores by Candida albicans was an established medical diagnostic test to confirm candidiasis before the molecular era. However, the functional role and pathological relevance of this in vitro morphological transition to pathogenesis in vivo remain unclear. We compared the physical properties of in vitro-induced chlamydospores with those of large C. albicans cells purified by density gradient centrifugation from Candida-infected mouse kidneys. The morphological and physical properties of these cells in kidneys of mice infected intravenously with wild type C...
2016: PloS One
https://www.readbyqxmd.com/read/27679618/unveiling-the-synergistic-interaction-between-liposomal-amphotericin-b-and-colistin
#8
Rita Teixeira-Santos, Elisabete Ricardo, Ricardo J Branco, Maria M Azevedo, Acácio G Rodrigues, Cidália Pina-Vaz
Patients with multiple comorbidities are often administered simultaneously or sequentially antifungals and antibacterial agents, without full knowledge of the consequences of drug interactions. Considering the clinical relevance of liposomal amphotericin B (L-AMB), the association between L-AMB and six antibacterial agents was evaluated against four clinical isolates and one type strain of Candida spp. and two clinical isolates and one type strain of Aspergillus fumigatus. In order to evaluate such combined effects, the minimal inhibitory concentration (MIC) of L-AMB was determined in the presence of 0...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27667190/a-general-and-efficient-strategy-for-generating-the-stable-enzymes
#9
Xiao-Fei Zhang, Guang-Yu Yang, Yong Zhang, Yuan Xie, Stephen G Withers, Yan Feng
The local flexibility of an enzyme's active center plays pivotal roles in catalysis, however, little is known about how the flexibility of these flexible residues affects stability. In this study, we proposed an active center stabilization (ACS) strategy to improve the kinetic thermostability of Candida rugosa lipase1. Based on the B-factor ranking at the region ~10 Å within the catalytic Ser209, 18 residues were selected for site-saturation mutagenesis. Based on three-tier high-throughput screening and ordered recombination mutagenesis, the mutant VarB3 (F344I/F434Y/F133Y/F121Y) was shown to be the most stable, with a 40-fold longer in half-life at 60 °C and a 12...
September 26, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27544427/outbreak-of-candidemia-caused-by-fluconazole-resistant-candida-parapsilosis-strains-in-an-intensive-care-unit
#10
Henrique Marconi Sampaio Pinhati, Luiz Augusto Casulari, Ana Carolina Remondi Souza, Ricardo Andreotti Siqueira, Camila Maria Gomes Damasceno, Arnaldo Lopes Colombo
BACKGROUND: Candidemia is an increasing problem in tertiary care hospitals worldwide. Here, we report the first outbreak of candidemia caused by fluconazole-resistant C. parapsilosis (FRCP) strains in Brazil. METHODS: This was a cross-sectional study of clinical and microbiological data of all candidemic episodes diagnosed from July 2011 to February 2012 in a 200-bed tertiary care hospital. Initial yeast identification and susceptibility testing were performed using the VITEK 2 - System...
2016: BMC Infectious Diseases
https://www.readbyqxmd.com/read/27484020/discontinuation-of-echinocandin-and-azole-treatments-led-to-the-disappearance-of-an-fks-alteration-but-not-azole-resistance-during-clonal-candida-glabrata-persistent-candidaemia
#11
S Imbert, L Castain, A Pons, S Jacob, I Meyer, M Palous, C Vezinet, O Langeron, C Hennequin, A Monsel, A Fekkar
To give an indication of a fitness cost conferred by FKS mutation-associated echinocandin resistance in Candida glabrata during human infection. Six C. glabrata clinical strains sequentially isolated from blood and a hepatic abscess in a solid organ transplant recipient were analysed. The patient had received long-term azole and echinocandin therapy for invasive aspergillosis and persistent candidaemia. Minimal inhibitory concentrations were determined by the European Committee on Antimicrobial Susceptibility Testing (EUCAST) broth microdilution method...
July 30, 2016: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/27469174/yeasts-from-sub-antarctic-region-biodiversity-enzymatic-activities-and-their-potential-as-oleaginous-microorganisms
#12
A Martinez, I Cavello, G Garmendia, C Rufo, S Cavalitto, S Vero
Various microbial groups are well known to produce a range of extracellular enzymes and other secondary metabolites. However, the occurrence and importance of investment in such activities have received relatively limited attention in studies of Antarctic soil microbiota. Sixty-one yeasts strains were isolated from King George Island, Antarctica which were characterized physiologically and identified at the molecular level using the D1/D2 region of rDNA. Fifty-eight yeasts (belonging to the genera Cryptococcus, Leucosporidiella, Rhodotorula, Guehomyces, Candida, Metschnikowia and Debaryomyces) were screened for extracellular amylolytic, proteolytic, esterasic, pectinolytic, inulolytic xylanolytic and cellulolytic activities at low and moderate temperatures...
September 2016: Extremophiles: Life Under Extreme Conditions
https://www.readbyqxmd.com/read/27441664/compatibility-of-injectable-anticoagulant-agents-in-ethanol-in-vitro-antibiofilm-activity-and-impact-on-polyurethane-catheters-of-enoxaparin-400-u-ml-in-40-v-v-ethanol
#13
Damien Balestrino, Mercédès Quintana, Nicolas Charbonnel, Christiane Forestier, Claire Lartigue, Bertrand Souweine
BACKGROUND AND OBJECTIVES: Interdialytic lock solutions should maintain catheter patency and prevent catheter infections. We aimed to determine in which conditions injectable anticoagulant agents (IAAs) combined with ethanol are compatible and to assess the antibiofilm activity of the selected combination and its effects on dialysis catheters (DC). METHODS: The solubility and compatibility of unfractionated heparin (UFH), low molecular weight heparins (LMWHs), heparinoids and fondaparinux (50 to 2,500 U/mL) in 30 to 70% ethanol were determined by visual observation...
2016: PloS One
https://www.readbyqxmd.com/read/27411802/candida-vulturna-pro-tempore-sp-nov-a-dimorphic-yeast-species-related-to-the-candida-haemulonis-species-complex-isolated-from-flowers-and-clinical-sample
#14
Matthias Sipiczki, Ratna Mohd Tap
In a taxonomic study of yeasts isolated from flowers in Cagayan de Oro, Mindenao Island, The Philippines, strains were identified as representing Kabatiella microsticta, Metschnikowia koreensis and a hitherto undescribed dimorphic species. Sequences of the D1/D2 domains of the LSU 26S rRNA genes, the internal transcribed spacer (ITS) regions and the SSU 18S rRNA genes were identical in the strains of the last-named group and differed from the corresponding sequences of the type strain of the closest related species, Candida duobushaemulonii, by 4 % (D1/D2), 7 % (ITS) and 1 % (SSU)...
October 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27375091/immobilization-of-chlorophyll-by-using-layer-by-layer-technique-for-controlled-release-systems-and-photodynamic-inactivation
#15
Filipe D S Gorza, Romário J da Silva, Tarquin F Trescher, Graciela C Pedro, Maria A Oliveira de Sousa, Paula C S Souto, Josmary R Silva, Nara C de Souza
The development of systems for the controlled release of drugs is important because they allow the control of drug absorption and tissue distribution and also can reduce local toxicity. This study aimed to assemble and characterize two types of release systems, consisting of layer-by-layer films obtained from poly(allylamine) hydrochloride with chlorophyll (PAH/CHL films) or chlorophyll incorporated into dipalmitoylphosphatidylcholine liposomes (PAH/Lip+CHL films). For these systems, the molecular aggregation, growth process, thermally stimulated desorption, wettability, and controlling release of CHL was studied by using UV-vis spectroscopy and wetting contact angle analysis...
September 2016: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/27373684/alteramide-b-is-a-microtubule-antagonist-of-inhibiting-candida-albicans
#16
Yanjiao Ding, Yaoyao Li, Zhenyu Li, Juanli Zhang, Chunhua Lu, Haoxin Wang, Yuemao Shen, Liangcheng Du
BACKGROUND: Alteramide B (ATB), isolated from Lysobacter enzymogenes C3, was a new polycyclic tetramate macrolactam (PTM). ATB exhibited potent inhibitory activity against several yeasts, particularly Candida albicans SC5314, but its antifungal mechanism is unknown. METHODS: The structure of ATB was established by extensive spectroscopic analyses, including high-resolution mass spectrometry, 1D- and 2D-NMR, and CD spectra. Flow cytometry, fluorescence microscope, transmission electron microscope, molecular modeling, overexpression and site-directed mutation studies were employed to delineate the anti-Candida molecular mechanism of ATB...
October 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27320785/novel-aldimine-type-schiff-bases-of-4-amino-5-3-4-5-trimethoxyphenyl-methyl-1-2-4-triazole-3-thione-thiol-docking-study-synthesis-biological-evaluation-and-anti-tubulin-activity
#17
Alieh Ameri, Ghadamali Khodarahmi, Farshid Hassanzadeh, Hamid Forootanfar, Gholam-Hosein Hakimelahi
The present study was planned to design some novel aldimine-type Schiff bases bearing 3,4,5-trimethoxyphenyl and 1,2,4-triazole-3-thione/thiol as potential tubulin polymerization inhibitors. The obtained results of the molecular docking study using the tubulin complex (PDB code: 1SA0) showed that compounds H-25 and H-26 were well fitted in the colchicine binding site of tubulin with binding energies of -8.68 and -8.40 kcal/mol, respectively, in comparison to the main ligand (-8.20 kcal/mol). In parallel, molecular simulations were also performed on five other 3,4,5-trimethoxyphenyl-containing ligand targets including hsp90, VEGFR2, and human and microbial (Staphylococcus aureus and Candida albicans) dihydrofolate reductase, among which H-17, H-45, H-27, H-02, and H-19 were the most suitable compounds, respectively...
August 2016: Archiv der Pharmazie
https://www.readbyqxmd.com/read/27306260/the-effect-of-imidazolium-cations-on-the-structure-and-activity-of-the-candida-antarctica-lipase-b-enzyme-in-ionic-liquids
#18
Ho Shin Kim, Doyoung Eom, Yoon-Mo Koo, Yaroslava G Yingling
In order to understand how cations affect the structural changes and enzyme activity of Lipase B from Candida antarctica, we performed all-atom molecular dynamics simulations of CALB in four types of ionic liquids (ILs) with varying sizes of imidazolium cations and correlated these results with the experimentally determined CALB activity. The imidazolium cations under study differ in the alkyl tail length in the following order: [Emim](+) < [Bmim](+) < [Hmim](+) < [Omim](+). We observed that the best enzyme activity and structural stability of CALB are obtained in [Bmim][TfO] and [Hmim][TfO]...
August 10, 2016: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/27303714/upregulation-of-the-adhesin-gene-epa1-mediated-by-pdr1-in-candida-glabrata-leads-to-enhanced-host-colonization
#19
Luis A Vale-Silva, Beat Moeckli, Riccardo Torelli, Brunella Posteraro, Maurizio Sanguinetti, Dominique Sanglard
Candida glabrata is the second most common Candida species causing disseminated infection, after C. albicans. C. glabrata is intrinsically less susceptible to the widely used azole antifungal drugs and quickly develops secondary resistance. Resistance typically relies on drug efflux with transporters regulated by the transcription factor Pdr1. Gain-of-function (GOF) mutations in PDR1 lead to a hyperactive state and thus efflux transporter upregulation. Our laboratory has characterized a collection of C. glabrata clinical isolates in which azole resistance was found to correlate with increased virulence in vivo...
March 2016: MSphere
https://www.readbyqxmd.com/read/27251083/isolation-of-yeast-candidates-for-efficient-sophorolipids-production-their-production-potentials-associate-to-their-lineage
#20
Masaaki Konishi, Manako Fujita, Yu Ishibane, Yuki Shimizu, Yusuke Tsukiyama, Masashi Ishida
Eleven biosurfactant-producing strains were newly isolated from environmental samples using a drop-collapse assay and thin-layer chromatography (TLC). According to the TLC analysis, the separation patterns of the glycolipid spots of nine dominant strains corresponded to that of the sophorolipids produced by a Starmerella bombicola type strain. The retention factor values of the spot patterns of two strains were less than those of the others. Two representative major products were purified, and their molecular structures were determined...
October 2016: Bioscience, Biotechnology, and Biochemistry
keyword
keyword
13611
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"