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Candida molecular typing

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https://www.readbyqxmd.com/read/28497115/an-efficient-rapid-and-recyclable-system-for-crispr-mediated-genome-editing-in-candida-albicans
#1
Namkha Nguyen, Morgan M F Quail, Aaron D Hernday
Candida albicans is the most common fungal pathogen of humans. Historically, molecular genetic analysis of this important pathogen has been hampered by the lack of stable plasmids or meiotic cell division, limited selectable markers, and inefficient methods for generating gene knockouts. The recent development of clustered regularly interspaced short palindromic repeat(s) (CRISPR)-based tools for use with C. albicans has opened the door to more efficient genome editing; however, previously reported systems have specific limitations...
March 2017: MSphere
https://www.readbyqxmd.com/read/28490578/investigating-clinical-issues-by-genotyping-of-medically-important-fungi-why-and-how
#2
REVIEW
Alexandre Alanio, Marie Desnos-Ollivier, Dea Garcia-Hermoso, Stéphane Bretagne
Genotyping studies of medically important fungi have addressed elucidation of outbreaks, nosocomial transmissions, infection routes, and genotype-phenotype correlations, of which secondary resistance has been most intensively investigated. Two methods have emerged because of their high discriminatory power and reproducibility: multilocus sequence typing (MLST) and microsatellite length polymorphism (MLP) using short tandem repeat (STR) markers. MLST relies on single-nucleotide polymorphisms within the coding regions of housekeeping genes...
July 2017: Clinical Microbiology Reviews
https://www.readbyqxmd.com/read/28475320/structure-based-rational-design-of-novel-inhibitors-against-fructose-1-6-bisphosphate-aldolase-from-candida-albicans
#3
Xinya Han, Xiuyun Zhu, Zongqin Hong, Lin Wei, Yanliang Ren, Fen Wan, Shuaihua Zhu, Hao Peng, Li Guo, Li Rao, Lingling Feng, Jian Wan
Class II fructose-1,6-bisphosphate aldolases (FBA-II) are attractive new targets for the discovery of drugs to combat invasive fungal infection, because they are absent in animals and higher plants. Although several FBA-II inhibitors have been reported, none of these inhibitors exhibit antifungal effect so far. In this study, several novel inhibitors of FBA-II from C. albicans (Ca-FBA-II) with potent antifungal effects were rationally designed by jointly using a specific protocols of molecular docking-based virtual screening, accurate binding-conformation evaluation strategy, synthesis and enzymatic assays...
May 5, 2017: Journal of Chemical Information and Modeling
https://www.readbyqxmd.com/read/28456118/dna-interaction-antimicrobial-anticancer-activities-and-molecular-docking-study-of-some-new-vo-ii-cr-iii-mn-ii-and-ni-ii-mononuclear-chelates-encompassing-quaridentate-imine-ligand
#4
Laila H Abdel-Rahman, Ahmed M Abu-Dief, Moustafa O Aboelez, Azza A Hassan Abdel-Mawgoud
The present study was conducted to synthesis of some new imine Cr(III), VO(II), Mn(II) and Ni(II) complexes derived from the condensation of 2-amino phenol with 2-hydroxynapthaldehyde were synthesized. The prepared HNPN imine ligand was analyzed by its melting point, IR, (1)H NMR and (13)C NMR spectroscopies. The investigated HNPN imine complexes were characterized by elemental analysis, FT IR, UV-vis and thermal analysis (TGA) under nitrogen atmosphere from ambient temperature to 750°C. The experimental results revealed that the investigated complexes contain hydrated water molecules...
April 9, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28424474/molecular-epidemiology-and-azole-resistance-mechanism-study-of-candida-guilliermondii-from-a-chinese-surveillance-system
#5
Jing-Wei Cheng, Kang Liao, Timothy Kudinha, Shu-Ying Yu, Meng Xiao, He Wang, Fanrong Kong, Ying-Chun Xu
We studied the molecular epidemiology and mechanism of azole resistance of 164 C. guilliermondii isolates from a nationwide multi-center surveillance program. The isolates were identified by ITS gene sequencing, and the in vitro susceptibility to fluconazole and voriconazole was determined by broth microdilution method. The 14-α-demethylase gene ERG11 was amplified and sequenced, and microsatellite analysis was performed to study the genetic relatedness of the isolates. Amongst the 164 C. guilliermondii isolates, 15 (9...
April 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28413236/scopulariopsis-and-scopulariopsis-like-species-from-indoor-environments
#6
J H C Woudenberg, M Meijer, J Houbraken, R A Samson
Scopulariopsis-like species are often reported from the indoor environment, as well as from clinical samples. The lack of type isolates and thorough phylogenetic studies in the Microascaceae hampered the correct identification of these isolates. Based on recent phylogenetic studies, which resulted in multiple name changes, the aim is to molecularly identify the Scopulariopsis and scopulariopsis-like species which occur in the indoor environment and give an overview of the current species in these genera and their habitats...
September 2017: Studies in Mycology
https://www.readbyqxmd.com/read/28383598/genetic-relatedness-of-peri-implants-and-buccal-candida-albicans-isolates-determined-by-rapd-pcr
#7
Adriana M Bertone, Alcira C Rosa, Natalia Nastri, Hector D Santillán, Yamila Ariza, Cristina A Iovannitti, Virginia M Jewtuchowicz
Molecular techniques have been used in recent studies to identify a wide range of potential bacterial pathogens in periimplant pockets of the oral cavity. However, the prevalence and molecular epidemiology of yeasts and species distribution related to periimplantitis are as yet unknown. The aim of this study was to determine the prevalence and distribution of yeasts in periimplant biofilm and to study genetic relatedness of Candida albicans. Yeasts recovered from periimplant biofilm samples (n=89) and buccal samples (n=120) were studied in 40 immunocompetent nonsmoking patients who visited the dental clinic of the Asociación Implantodontológica Argentina, Buenos Aires, Argentina, and had received oral rehabilitation with implants for more than five years...
December 2016: Acta Odontológica Latinoamericana: AOL
https://www.readbyqxmd.com/read/28382028/notable-increasing-trend-in-azole-non-susceptible-candida-tropicalis-causing-invasive-candidiasis-in-china-august-2009-to-july-2014-molecular-epidemiology-and-clinical-azole-consumption
#8
Xin Fan, Meng Xiao, Kang Liao, Timothy Kudinha, He Wang, Li Zhang, Xin Hou, Fanrong Kong, Ying-Chun Xu
Objectives: To report the notable increasing trends of C. tropicalis antifungal resistance in the past 5 years, and explore molecular epidemiology, and the relationship between clinical azoles consumption and increased resistance rate. Methods: Between August 2009 and July 2014, 507 non-duplicated C. tropicalis isolates causing invasive candidiasis were collected from 10 hospitals in China. The in vitro antifungal susceptibility of nine common agents was determined by Sensititre YeastOne™ using current available species-specific clinical breakpoint (CBPs) or epidemiological cut-off values (ECVs)...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28380072/population-structure-and-molecular-genetic-characterization-of-5-flucytosine-susceptible-and-resistant-clinical-candida-dubliniensis-isolates-from-kuwait
#9
Mohammad Asadzadeh, Suhail Ahmad, Noura Al-Sweih, Ziauddin Khan
Candida dubliniensis and Candida albicans are two closely related species. Although C. dubliniensis is less pathogenic, it has a higher propensity to develop resistance to fluconazole and some strains exhibit intrinsic resistance to 5-flucytosine (5-FC). All 5-FC-resistant isolates from Kuwait were previously shown to belong to one of seven internal transcribed spacer (ITS) region of rDNA-based haplotypes. This study performed fingerprinting of C. dubliniensis isolates by multilocus sequence typing (MLST) to determine population structure of 5-FC-resistant and -susceptible strains and compared the results with data from a global collection of isolates...
2017: PloS One
https://www.readbyqxmd.com/read/28364622/identification-and-characterization-of-lactic-acid-bacteria-and-yeasts-of-pdo-tuscan-bread-sourdough-by-culture-dependent-and-independent-methods
#10
Michela Palla, Caterina Cristani, Manuela Giovannetti, Monica Agnolucci
Sourdough fermentation has been increasingly used worldwide, in accordance with the demand of consumers for tasty, natural and healthy food. The high diversity of lactic acid bacteria (LAB) and yeast species, detected in sourdoughs all over the world, may affect nutritional, organoleptic and technological traits of leavened baked goods. A wide regional variety of traditional sourdough breads, over 200 types, has been recorded in Italy, including special types selected as worthy of either Protected Geographical Indication (PGI) or Protected Designation of Origin (PDO), whose sourdough microbiota has been functionally and molecularly characterized...
March 23, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28343242/identification-of-a-polysaccharide-produced-by-the-pyruvate-overproducer-candida-glabrata-cctcc-m202019
#11
Zhengshan Luo, Song Liu, Guocheng Du, Jingwen Zhou, Jian Chen
Candida glabrata has great potential for the accumulation of pyruvate as a preferred strain in pyruvate production by fermentation. However, its substrate conversion rate is relatively low. In this study, a novel polysaccharide containing α-1,4-glucosidic bonds was observed accidentally in screening a high-titer pyruvate strain by atmospheric and room temperature plasma mutagenesis of C. glabrata. Chemical analysis of the partially purified polysaccharide S4-C10 showed the main components were 1.2% (w/w) protein and 94...
March 25, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28270801/molecular-fingerprinting-studies-do-not-support-intrahospital-transmission-of-candida-albicans-among-candidemia-patients-in-kuwait
#12
Mohammad Asadzadeh, Suhail Ahmad, Noura Al-Sweih, Ziauddin Khan
Candida albicans, a constituent of normal microbial flora of human mucosal surfaces, is a major cause of candidemia in immunocompromised individuals and hospitalized patients with other debilitating diseases. Molecular fingerprinting studies have suggested nosocomial transmission of C. albicans based on the presence of clusters or endemic genotypes in some hospitals. However, intrahospital strain transmission or a common source of infection has not been firmly established. We performed multilocus sequence typing (MLST) on 102 C...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28257640/risk-factors-for-candida-parapsilosis-bloodstream-infection-in-a-neonatal-intensive-care-unit-a-case-control-study
#13
Carmine Garzillo, Maria Bagattini, Lidija Bogdanović, Anna Di Popolo, Vita Dora Iula, Maria Rosaria Catania, Francesco Raimondi, Maria Triassi, Raffaele Zarrilli
BACKGROUND: Candida parapsilosis is increasingly responsible for invasive candidiasis in neonates. This study investigates phenotypic and genotypic features of C. parapsilosis microbial isolates and underlying clinical conditions associated with acquisition of C. parapsilosis in a neonatal intensive care unit (NICU) in Italy. METHODS: Identification of C. parapsilosis was performed by VITEK® 2 and MALDI TOF and confirmed by analysis of internal transcribed spacer ribosomal DNA sequences...
January 19, 2017: Italian Journal of Pediatrics
https://www.readbyqxmd.com/read/28228111/antimicrobial-structure-activity-relationship-of-five-anthraquinones-of-emodine-type-isolated-from-vismia-laurentii
#14
Gislaine Aurelie Kemegne, Pierre Mkounga, Jean Justin Essia Ngang, Sylvain Leroy Sado Kamdem, Augustin Ephrem Nkengfack
BACKGROUND: Antimicrobial activity of anthraquinone compounds of emodine type has been reported by many authors. These compounds are found in Vismia laurentii (Clusiaceae), a plant used in traditional pharmacopoeia for treatment of microbial infections among others affections. The continuous identification of new compounds has raised the problem of the relation between the structure and antimicrobial properties. RESULTS: The yeast growth kinetics parameters were not influenced by the pH variation as it was the case for the other tested bacteria...
February 22, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28225905/comparison-of-four-molecular-approaches-to-identify-candida-parapsilosis-complex-species
#15
Leonardo Silva Barbedo, Maria Helena Galdino Figueiredo-Carvalho, Mauro de Medeiros Muniz, Rosely Maria Zancopé-Oliveira
Since the description of Candida orthopsilosis and C. metapsilosis in 2005, several methods have been proposed to identify and differentiate these species from C. parapsilosis sensu stricto. Species-specific uniplex polymerase chain reaction (PCR) was performed and compared with sequencing of the D1/D2 region of the LSU 28S rDNA gene, microsatellite typing of C. parapsilosis sensu stricto, and PCR-restriction fragment length polymorphism patterns in the ITS1-5.8S-ITS2 region of the rDNA gene. There was agreement between results of testing of 98 clinical isolates with the four PCR-based methods, with 59 isolates identified as C...
March 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28225899/comparison-of-four-molecular-approaches-to-identify-candida-parapsilosis-complex-species
#16
Leonardo Silva Barbedo, Maria Helena Galdino Figueiredo-Carvalho, Mauro de Medeiros Muniz, Rosely Maria Zancopé-Oliveira
Since the description of Candida orthopsilosis and C. metapsilosis in 2005, several methods have been proposed to identify and differentiate these species from C. parapsilosis sensu stricto. Species-specific uniplex polymerase chain reaction (PCR) was performed and compared with sequencing of the D1/D2 region of the LSU 28S rDNA gene, microsatellite typing of C. parapsilosis sensu stricto, and PCR-restriction fragment length polymorphism patterns in the ITS1-5.8S-ITS2 region of the rDNA gene. There was agreement between results of testing of 98 clinical isolates with the four PCR-based methods, with 59 isolates identified as C...
February 16, 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28183985/characterization-of-pph3-mediated-dephosphorylation-of-rad53-during-methyl-methanesulfonate-induced-dna-damage-repair-in-candida-albicans
#17
Guangyin Yao, Junhua Wan, Qizheng Liu, Chunhua Mu, Yue Wang, Jianli Sang
Genotoxic stress causes DNA damage or stalled DNA replication and filamentous growth in the pathogenic fungus Candida albicans The DNA checkpoint kinase Rad53 critically regulates by phosphorylation effectors that execute the stress response. Rad53 itself is activated by phosphorylation and inactivated by dephosphorylation. Previous studies have suggested that the phosphatase Pph3 dephosphorylates Rad53. Here, we used mass spectrometry and mutagenesis to identify Pph3 dephosphorylation sites on Rad53 in C. albicans We found that serine residues 351, 461, and 477, which were dephosphorylated in wild-type cells during the recovery from DNA damage caused by methyl methanesulfonate (MMS), remained phosphorylated in pph3Δ/Δ cells...
February 9, 2017: Biochemical Journal
https://www.readbyqxmd.com/read/28154553/identification-and-antifungal-susceptibility-profiles-of-candida-nivariensis-and-candida-bracarensis-in-a-multi-center-chinese-collection-of-yeasts
#18
Xin Hou, Meng Xiao, Sharon C-A Chen, He Wang, Shu-Ying Yu, Xin Fan, Fanrong Kong, Ying-Chun Xu
Candida nivariensis and C. bracarensis are two emerging cryptic species within the C. glabrata complex. Thirteen of these isolates from 10 hospitals in China were studied for their species identification and antifungal susceptibilities. Phenotypic and molecular [rDNA ITS sequencing, D1/D2 sequencing and ITS sequencer-based capillary gel electrophoresis (SCGE)] and matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) MS identification methods were compared for their performance in species identification...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28115353/in-vitro-antifungal-susceptibility-testing-of-candida-isolates-with-the-eucast-methodology-a-new-method-for-ecoff-determination
#19
J Meletiadis, I Curfs-Breuker, J F Meis, J W Mouton
The in vitro susceptibilities of 1,099 molecularly identified clinical Candida isolates against 8 antifungal drugs were determined using the EUCAST microdilution method. A new simple, objective, and mathematically solid method for determining epidemiological cutoff values (ECOFFs) was developed by derivatizing the MIC distribution and determining the derivatized ECOFF (dECOFF) as the highest MIC with the maximum second derivative. The dECOFFs were similar (95% agreement within 1 dilution) to the EUCAST ECOFFs...
April 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28011958/cationic-porphyrin-mediated-photodynamic-inactivation-of-candida-biofilms-and-the-effect-of-miconazole
#20
A Davies, S Gebremedhin, M Yee, R J Padilla, N Duzgunes, K Konopka, B Dorocka-Bobkowska
The formation of biofilms by Candida and the increasing resistance of Candida species to antifungals contribute to the high recurrence rates of denture stomatitis. This increase has stimulated an interest in antimicrobial photodynamic therapy (aPDT) as an alternative treatment. We examined the photoactivity of the porphyrin-based photosensitizer, TMP-1363, against biofilms of C. albicans, C. glabrata, C. tropicalis and C. parapsilosis, and the effect of the combined use of miconazole and aPDT. Biofilms of three American Type Culture Collection (ATCC) strains and four clinical isolates developed on poly(methyl methacrylate) (PMMA) disks, were incubated with miconazole, followed by treatment with TMP-1363 for 30 min at 37°C...
October 2016: Journal of Physiology and Pharmacology: An Official Journal of the Polish Physiological Society
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