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Candida molecular typing

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https://www.readbyqxmd.com/read/28918151/internal-transcribed-spacer-its-sequencing-reveals-considerable-fungal-diversity-in-dairy-products
#1
A J Buehler, R L Evanowski, N H Martin, K J Boor, M Wiedmann
Fungi are important spoilage organisms in dairy products. However, little is known about the diversity of naturally occurring spoilage fungi in raw milk and processed dairy products, due at least in part to the fact that classical fungal identification methods require considerable expertise. To gain further insight into the fungal diversity in the dairy system, we isolated fungi from raw milk, raw and pasteurized milk cheese, and yogurt using the selective dichloran rose bengal chloramphenicol agar. In total, 361 fungal isolates were obtained and further characterized by DNA sequencing of the internal transcribed spacer (ITS) region and the nuclear ribosomal large subunit (LSU) rRNA gene if needed...
September 13, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28854190/population-structure-and-molecular-genetic-characterization-of-clinical-candida-tropicalis-isolates-from-a-tertiary-care-hospital-in-kuwait-reveal-infections-with-unique-strains
#2
Khaled Al-Obaid, Mohammad Asadzadeh, Suhail Ahmad, Ziauddin Khan
Candida tropicalis is a frequently isolated yeast species causing bloodstream, urinary tract and other infections particularly in patients admitted to intensive care units (ICUs) and those requiring prolonged urinary catheterization (UC) or receiving broad-spectrum antibiotics (BSA). This study investigated clinical characteristics and genetic relatedness among C. tropicalis strains isolated from patients at Al-Amiri Hospital in Kuwait. C. tropicalis strains (n = 63) isolated from blood, genito-urinary, respiratory (RT) and digestive (GIT) tracts and wound sites from 54 patients were used...
2017: PloS One
https://www.readbyqxmd.com/read/28843335/the-identification-of-meyerozyma-guilliermondii-from-blood-cultures-and-surveillance-samples-in-a-university-hospital-in-northeast-turkey-a-ten-year-survey
#3
N Cebeci Güler, I Tosun, F Aydin
Meyerozyma (Pichia) guilliermondii exists in human skin and mucosal surface microflora. It can cause severe fungal infections like candidemia, which is an opportunistic pathogen. One hundred and forty-one M. guilliermondii isolates, consisting of 122 blood culture isolates, belonging to 126 patients; 13 total parenteral nutrition solution isolates; and two rectal swab isolates were identified according to carbohydrate assimilation reactions in a university hospital in Turkey between January 2006 and December 2015...
August 23, 2017: Journal de Mycologie Médicale
https://www.readbyqxmd.com/read/28837732/candida-species-from-eye-infections-drug-susceptibility-virulence-factors-and-molecular-characterization
#4
Konduri Ranjith, Bhavani Sontam, Savitri Sharma, Joveeta Joseph, Kanchana N Chathoth, Kalyana C Sama, Somasheila I Murthy, Sisinthy Shivaji
Purpose: To determine the type of Candida species in ocular infections and to investigate the relationship of antifungal susceptibility profile to virulence factors. Methods: Fifty isolates of yeast-like fungi from patients with keratitis, endophthalmitis, and orbital cellulitis were identified by Vitek-2 compact system and DNA sequencing of ITS1-5.8S-ITS2 regions of the rRNA gene, followed by phylogenetic analysis for phenotypic and genotypic identification, respectively...
August 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28821607/two-plant-derived-aporphinoid-alkaloids-exert-their-antifungal-activity-by-disrupting-mitochondrial-iron-sulfur-cluster-biosynthesis
#5
Siddharth K Tripathi, Tao Xu, Qin Feng, Bharathi Avula, Xiaomin Shi, Xuewen Pan, Melanie M Mask, Scott R Baerson, Melissa R Jacob, Ranga Rao Ravu, Shabana I Khan, Xing-Cong Li, Ikhlas A Khan, Alice M Clark, Ameeta K Agarwal
Eupolauridine and liriodenine are plant-derived aporphinoid alkaloids that exhibit potent inhibitory activity against the opportunistic fungal pathogens Candida albicans and Cryptococcus neoformans However, the molecular mechanism of this antifungal activity is unknown. In this study, we show that eupolauridine 9591 (E9591), a synthetic analog of eupolauridine, and liriodenine methiodide (LMT), a methiodide salt of liriodenine, mediate their antifungal activities by disrupting mitochondrial iron-sulfur (Fe-S) cluster synthesis...
August 18, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28808688/adaptive-mistranslation-accelerates-the-evolution-of-fluconazole-resistance-and-induces-major-genomic-and-gene-expression-alterations-in-candida-albicans
#6
Tobias Weil, Rodrigo Santamaría, Wanseon Lee, Johan Rung, Noemi Tocci, Darren Abbey, Ana R Bezerra, Laura Carreto, Gabriela R Moura, Mónica Bayés, Ivo G Gut, Attila Csikasz-Nagy, Duccio Cavalieri, Judith Berman, Manuel A S Santos
Regulated erroneous protein translation (adaptive mistranslation) increases proteome diversity and produces advantageous phenotypic variability in the human pathogen Candida albicans. It also increases fitness in the presence of fluconazole, but the underlying molecular mechanism is not understood. To address this question, we evolved hypermistranslating and wild-type strains in the absence and presence of fluconazole and compared their fluconazole tolerance and resistance trajectories during evolution. The data show that mistranslation increases tolerance and accelerates the acquisition of resistance to fluconazole...
July 2017: MSphere
https://www.readbyqxmd.com/read/28772020/interplay-between-lactobacillus-rhamnosus-gg-and-candida-and-the-involvement-of-exopolysaccharides
#7
Camille N Allonsius, Marianne F L van den Broek, Ilke De Boeck, Shari Kiekens, Eline F M Oerlemans, Filip Kiekens, Kenn Foubert, Dieter Vandenheuvel, Paul Cos, Peter Delputte, Sarah Lebeer
A number of clinical studies have shown protective effects of lactobacilli against Candida species in the gastrointestinal tract, the urogenital tract and the oral cavity, while others did not show clear effects. Evidence on the mode of action of lactobacilli against Candida is also still lacking. In this study, the anti-Candida activity of the model probiotic strain Lactobacillus rhamnosus GG was explored in different assays to determine molecular interactions. We found that L. rhamnosus GG was able to interfere with Candida growth, morphogenesis and adhesion...
August 3, 2017: Microbial Biotechnology
https://www.readbyqxmd.com/read/28770873/prevalence-and-antifungal-susceptibility-of-candida-species-in-a-tertiary-care-hospital-in-islamabad-pakistan
#8
Umber Tasneem, Maryam Tahir Siddiqui, Rani Faryal, Aamer Ali Shah
OBJECTIVE: To determine the prevalence and antifungal susceptibility pattern of Candida species. METHODS: This prospective, cross-sectional study was conducted at the Quaid-e-Azam International Hospital, Islamabad, Pakistan, from January 2014 to February 2015, and comprised different clinical samples which were analysed for various types of microbial infections. Species differentiation was confirmed by biochemical and molecular methods. Antifungal susceptibility against amphotericin B, fluconazole and voriconazole was determined by Clinical and Laboratory Standards Institute M44-A disk diffusion method...
July 2017: JPMA. the Journal of the Pakistan Medical Association
https://www.readbyqxmd.com/read/28616054/an-investigation-on-non-invasive-fungal-sinusitis-molecular-identification-of-etiologic-agents
#9
Abdolrasoul Mohammadi, Seyed Mostafa Hashemi, Sayed Hamidreza Abtahi, Seyed Mohammad Lajevardi, Sahar Kianipour, Rasoul Mohammadi
BACKGROUND: Fungal sinusitis is increasing worldwide in the past two decades. It is divided into two types including invasive and noninvasive. Noninvasive types contain allergic fungal sinusitis (AFS) and fungus ball. AFS is a hypersensitivity reaction to fungal allergens in the mucosa of the sinonasal tract in atopic individuals. The fungus ball is a different type of noninvasive fungal rhinosinusitis which is delineated as an accumulation of debris and fungal elements inside a paranasal sinus...
2017: Journal of Research in Medical Sciences: the Official Journal of Isfahan University of Medical Sciences
https://www.readbyqxmd.com/read/28596548/sortase-a-mediated-crosslinked-short-chain-dehydrogenases-reductases-as-novel-biocatalysts-with-improved-thermostability-and-catalytic-efficiency
#10
Kunpeng Li, Rongzhen Zhang, Yan Xu, Zhimeng Wu, Jing Li, Xiaotian Zhou, Jiawei Jiang, Haiyan Liu, Rong Xiao
(S)-carbonyl reductase II (SCRII) from Candida parapsilosis is a short-chain alcohol dehydrogenase/reductase. It catalyses the conversion of 2-hydroxyacetophenone to (S)-1-phenyl-1,2-ethanediol with low efficiency. Sortase was reported as a molecular "stapler" for site-specific protein conjugation to strengthen or add protein functionality. Here, we describe Staphylococcus aureus sortase A-mediated crosslinking of SCRII to produce stable catalysts for efficient biotransformation. Via a native N-terminal glycine and an added GGGGSLPETGG peptide at C-terminus of SCRII, SCRII subunits were conjugated by sortase A to form crosslinked SCRII, mainly dimers and trimers...
June 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28588560/molecular-epidemiology-and-antifungal-susceptibility-of-candida-glabrata-in-china-august-2009-to-july-2014-a-multi-center-study
#11
Xin Hou, Meng Xiao, Sharon C-A Chen, Fanrong Kong, He Wang, Yun-Zhuo Chu, Mei Kang, Zi-Yong Sun, Zhi-Dong Hu, Ruo-Yu Li, Juan Lu, Kang Liao, Tie-Shi Hu, Yu-Xing Ni, Gui-Ling Zou, Ge Zhang, Xin Fan, Yu-Pei Zhao, Ying-Chun Xu
Candida glabrata is an increasingly important cause of invasive candidiasis. In China, relatively little is known of the molecular epidemiology of C. glabrata and of its antifungal susceptibility patterns. Here we studied 411 non-duplicate C. glabrata isolates from 411 patients at 11 hospitals participating in the National China Hospital Invasive Fungal Surveillance Net program (CHIF-NET; 2010-2014). Genotyping was performed using multilocus sequence typing (MLST) employing six genetic loci and by microsatellite analysis...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28587312/molecular-typing-of-staphylococcus-aureus-isolated-from-patients-with-autosomal-dominant-hyper-ige-syndrome
#12
Inka Sastalla, Kelli W Williams, Erik D Anderson, Ian A Myles, Jensen D Reckhow, Marlene Espinoza-Moraga, Alexandra F Freeman, Sandip K Datta
Autosomal dominant hyper IgE syndrome (AD-HIES) is a primary immunodeficiency caused by a loss-of-function mutation in the Signal Transducer and Activator of Transcription 3 (STAT3). This immune disorder is clinically characterized by increased susceptibility to cutaneous and sinopulmonary infections, in particular with Candida and Staphylococcus aureus. It has recently been recognized that the skin microbiome of patients with AD-HIES is altered with an overrepresentation of certain Gram-negative bacteria and Gram-positive staphylococci...
June 6, 2017: Pathogens
https://www.readbyqxmd.com/read/28559186/multidrug-abc-transporter-cdr1-of-candida-albicans-harbors-specific-and-overlapping-binding-sites-for-human-steroid-hormones-transport
#13
Pratima Baghel, Manpreet Kaur Rawal, Mohammad Firoz Khan, Sobhan Sen, Mohammed Haris Siddiqui, Vincent Chaptal, Pierre Falson, Rajendra Prasad
The present study examines the kinetics of steroids efflux mediated by the Candida drug resistance protein 1 (Cdr1p) and evaluates their interaction with the protein. We exploited our in-house mutant library for targeting the 252 residues forming the twelve transmembrane helices (TMHs) of Cdr1p. The screening revealed 65 and 58 residues critical for β-estradiol and corticosterone transport, respectively. Notably, up to 83% critical residues for corticosterone face the lipid interface compared to 54% for β-estradiol...
May 27, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28497115/an-efficient-rapid-and-recyclable-system-for-crispr-mediated-genome-editing-in-candida-albicans
#14
Namkha Nguyen, Morgan M F Quail, Aaron D Hernday
Candida albicans is the most common fungal pathogen of humans. Historically, molecular genetic analysis of this important pathogen has been hampered by the lack of stable plasmids or meiotic cell division, limited selectable markers, and inefficient methods for generating gene knockouts. The recent development of clustered regularly interspaced short palindromic repeat(s) (CRISPR)-based tools for use with C. albicans has opened the door to more efficient genome editing; however, previously reported systems have specific limitations...
March 2017: MSphere
https://www.readbyqxmd.com/read/28490578/investigating-clinical-issues-by-genotyping-of-medically-important-fungi-why-and-how
#15
REVIEW
Alexandre Alanio, Marie Desnos-Ollivier, Dea Garcia-Hermoso, Stéphane Bretagne
Genotyping studies of medically important fungi have addressed elucidation of outbreaks, nosocomial transmissions, infection routes, and genotype-phenotype correlations, of which secondary resistance has been most intensively investigated. Two methods have emerged because of their high discriminatory power and reproducibility: multilocus sequence typing (MLST) and microsatellite length polymorphism (MLP) using short tandem repeat (STR) markers. MLST relies on single-nucleotide polymorphisms within the coding regions of housekeeping genes...
July 2017: Clinical Microbiology Reviews
https://www.readbyqxmd.com/read/28475320/structure-based-rational-design-of-novel-inhibitors-against-fructose-1-6-bisphosphate-aldolase-from-candida-albicans
#16
Xinya Han, Xiuyun Zhu, Zongqin Hong, Lin Wei, Yanliang Ren, Fen Wan, Shuaihua Zhu, Hao Peng, Li Guo, Li Rao, Lingling Feng, Jian Wan
Class II fructose-1,6-bisphosphate aldolases (FBA-II) are attractive new targets for the discovery of drugs to combat invasive fungal infection, because they are absent in animals and higher plants. Although several FBA-II inhibitors have been reported, none of these inhibitors exhibit antifungal effect so far. In this study, several novel inhibitors of FBA-II from C. albicans (Ca-FBA-II) with potent antifungal effects were rationally designed by jointly using a specific protocols of molecular docking-based virtual screening, accurate binding-conformation evaluation strategy, synthesis and enzymatic assays...
June 26, 2017: Journal of Chemical Information and Modeling
https://www.readbyqxmd.com/read/28456118/dna-interaction-antimicrobial-anticancer-activities-and-molecular-docking-study-of-some-new-vo-ii-cr-iii-mn-ii-and-ni-ii-mononuclear-chelates-encompassing-quaridentate-imine-ligand
#17
Laila H Abdel-Rahman, Ahmed M Abu-Dief, Moustafa O Aboelez, Azza A Hassan Abdel-Mawgoud
The present study was conducted to synthesis of some new imine Cr(III), VO(II), Mn(II) and Ni(II) complexes derived from the condensation of 2-amino phenol with 2-hydroxynapthaldehyde were synthesized. The prepared HNPN imine ligand was analyzed by its melting point, IR, (1)H NMR and (13)C NMR spectroscopies. The investigated HNPN imine complexes were characterized by elemental analysis, FT IR, UV-vis and thermal analysis (TGA) under nitrogen atmosphere from ambient temperature to 750°C. The experimental results revealed that the investigated complexes contain hydrated water molecules...
May 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28424474/molecular-epidemiology-and-azole-resistance-mechanism-study-of-candida-guilliermondii-from-a-chinese-surveillance-system
#18
Jing-Wei Cheng, Kang Liao, Timothy Kudinha, Shu-Ying Yu, Meng Xiao, He Wang, Fanrong Kong, Ying-Chun Xu
We studied the molecular epidemiology and mechanism of azole resistance of 164 C. guilliermondii isolates from a nationwide multi-center surveillance program. The isolates were identified by ITS gene sequencing, and the in vitro susceptibility to fluconazole and voriconazole was determined by broth microdilution method. The 14-α-demethylase gene ERG11 was amplified and sequenced, and microsatellite analysis was performed to study the genetic relatedness of the isolates. Amongst the 164 C. guilliermondii isolates, 15 (9...
April 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28413236/scopulariopsis-and-scopulariopsis-like-species-from-indoor-environments
#19
J H C Woudenberg, M Meijer, J Houbraken, R A Samson
Scopulariopsis-like species are often reported from the indoor environment, as well as from clinical samples. The lack of type isolates and thorough phylogenetic studies in the Microascaceae hampered the correct identification of these isolates. Based on recent phylogenetic studies, which resulted in multiple name changes, the aim is to molecularly identify the Scopulariopsis and scopulariopsis-like species which occur in the indoor environment and give an overview of the current species in these genera and their habitats...
September 2017: Studies in Mycology
https://www.readbyqxmd.com/read/28383598/genetic-relatedness-of-peri-implants-and-buccal-candida-albicans-isolates-determined-by-rapd-pcr
#20
Adriana M Bertone, Alcira C Rosa, Natalia Nastri, Hector D Santillán, Yamila Ariza, Cristina A Iovannitti, Virginia M Jewtuchowicz
Molecular techniques have been used in recent studies to identify a wide range of potential bacterial pathogens in periimplant pockets of the oral cavity. However, the prevalence and molecular epidemiology of yeasts and species distribution related to periimplantitis are as yet unknown. The aim of this study was to determine the prevalence and distribution of yeasts in periimplant biofilm and to study genetic relatedness of Candida albicans. Yeasts recovered from periimplant biofilm samples (n=89) and buccal samples (n=120) were studied in 40 immunocompetent nonsmoking patients who visited the dental clinic of the Asociación Implantodontológica Argentina, Buenos Aires, Argentina, and had received oral rehabilitation with implants for more than five years...
December 2016: Acta Odontológica Latinoamericana: AOL
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