keyword
MENU ▼
Read by QxMD icon Read
search

Fungi molecular typing

keyword
https://www.readbyqxmd.com/read/28536287/kisameet-glacial-clay-an-unexpected-source-of-bacterial-diversity
#1
Sarah L Svensson, Shekooh Behroozian, Wanjing Xu, Michael G Surette, Loretta Li, Julian Davies
Widespread antibiotic resistance among bacterial pathogens is providing the impetus to explore novel sources of antimicrobial agents. Recently, the potent antibacterial activity of certain clay minerals has stimulated scientific interest in these materials. One such example is Kisameet glacial clay (KC), an antibacterial clay from a deposit on the central coast of British Columbia, Canada. However, our understanding of the active principles of these complex natural substances is incomplete. Like soils, clays may possess complex mixtures of bacterial taxa, including the Actinobacteria, a clade known to be rich in antibiotic-producing organisms...
May 23, 2017: MBio
https://www.readbyqxmd.com/read/28527583/molecular-characterization-of-aspergillus-fumigatus-tcsc-a-characteristic-type-iii-hybrid-histidine-kinase-of-filamentous-fungi-harboring-six-hamp-domains
#2
Anja Spadinger, Frank Ebel
The type III hybrid histidine kinase (HHK) TcsC enables the pathogenic mold Aspergillus fumigatus to thrive under hyperosmotic conditions. It is, moreover, of particular interest, since it is the target of certain antifungal agents, such as fludioxonil. This study was aimed at a functional characterization of the domains that constitute the sensing and the kinase module of TcsC. The sensing module consists of six HAMP domains, an architecture that is commonly found in type III HHKs of filamentous fungi. To dissect the functional role of the individual domains, we have analyzed a set of truncated derivatives of TcsC with respect to their impact on fungal growth and their ability to respond to hyperosmotic stress and fludioxonil...
May 11, 2017: International Journal of Medical Microbiology: IJMM
https://www.readbyqxmd.com/read/28527395/hydrogen-bonded-charge-transfer-molecular-salt-4-chloro-anilinium-3-nitrophthalate-for-photophysical-and-pharmacological-applications
#3
K Singaravelan, A Chandramohan, M Saravanabhavan, I V Muthu Vijayan Enoch, V S Suganthi
Radical scavenging activity against DPPH radical and binding properties of a hydrogen bonded charge transfer molecular salt 4-chloro anilinium-3-nitrophthalate(CANP) with calf thymus DNA has been studied by electronic absorption and emission spectroscopy. The molecular structure and crystallinity of the CANP salt have been established by carried out powder and single crystal X-ray diffraction analysis which indicated that cation and anion are linked through strong N(+)H(…)O(-) type of hydrogen bond. FTIR spectroscopic study was carried out to know the various functional groups present in the crystal...
May 10, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28527179/making-time-conservation-of-biological-clocks-from-fungi-to-animals
#4
Jay C Dunlap, Jennifer J Loros
The capacity for biological timekeeping arose at least three times through evolution, in prokaryotic cyanobacteria, in cells that evolved into higher plants, and within the group of organisms that eventually became the fungi and the animals. Neurospora is a tractable model system for understanding the molecular bases of circadian rhythms in the last of these groups, and is perhaps the most intensively studied circadian cell type. Rhythmic processes described in fungi include growth rate, stress responses, developmental capacity, and sporulation, as well as much of metabolism; fungi use clocks to anticipate daily environmental changes...
May 2017: Microbiology Spectrum
https://www.readbyqxmd.com/read/28520621/outbreaks-in-the-adult-icus
#5
Hasan M Al-Dorzi, Yaseen M Arabi
PURPOSE OF REVIEW: Infectious disease outbreaks in the critical care setting are common and serious consequence. This article reviews and summarizes recent outbreaks in the adult ICUs. RECENT FINDINGS: Outbreaks of multidrug-resistant bacteria, fungi, and emerging viruses in ICUs from different countries are common. Outbreak investigation relies on epidemiologic methods, microbiologic studies, and molecular typing methods. Overuse of antibiotics, gaps in implementing infection prevention measures, and contaminated environment are common causes of ICU outbreaks...
May 17, 2017: Current Opinion in Infectious Diseases
https://www.readbyqxmd.com/read/28490578/investigating-clinical-issues-by-genotyping-of-medically-important-fungi-why-and-how
#6
REVIEW
Alexandre Alanio, Marie Desnos-Ollivier, Dea Garcia-Hermoso, Stéphane Bretagne
Genotyping studies of medically important fungi have addressed elucidation of outbreaks, nosocomial transmissions, infection routes, and genotype-phenotype correlations, of which secondary resistance has been most intensively investigated. Two methods have emerged because of their high discriminatory power and reproducibility: multilocus sequence typing (MLST) and microsatellite length polymorphism (MLP) using short tandem repeat (STR) markers. MLST relies on single-nucleotide polymorphisms within the coding regions of housekeeping genes...
July 2017: Clinical Microbiology Reviews
https://www.readbyqxmd.com/read/28462022/exploring-the-binding-properties-and-structural-stability-of-an-opsin-in-the-chytrid-spizellomyces-punctatus-using-comparative-and-molecular-modeling
#7
Steven R Ahrendt, Edgar Mauricio Medina, Chia-En A Chang, Jason E Stajich
BACKGROUND: Opsin proteins are seven transmembrane receptor proteins which detect light. Opsins can be classified into two types and share little sequence identity: type 1, typically found in bacteria, and type 2, primarily characterized in metazoa. The type 2 opsins (Rhodopsins) are a subfamily of G-protein coupled receptors (GPCRs), a large and diverse class of seven transmembrane proteins and are generally restricted to metazoan lineages. Fungi use light receptors including opsins to sense the environment and transduce signals for developmental or metabolic changes...
2017: PeerJ
https://www.readbyqxmd.com/read/28456118/dna-interaction-antimicrobial-anticancer-activities-and-molecular-docking-study-of-some-new-vo-ii-cr-iii-mn-ii-and-ni-ii-mononuclear-chelates-encompassing-quaridentate-imine-ligand
#8
Laila H Abdel-Rahman, Ahmed M Abu-Dief, Moustafa O Aboelez, Azza A Hassan Abdel-Mawgoud
The present study was conducted to synthesis of some new imine Cr(III), VO(II), Mn(II) and Ni(II) complexes derived from the condensation of 2-amino phenol with 2-hydroxynapthaldehyde were synthesized. The prepared HNPN imine ligand was analyzed by its melting point, IR, (1)H NMR and (13)C NMR spectroscopies. The investigated HNPN imine complexes were characterized by elemental analysis, FT IR, UV-vis and thermal analysis (TGA) under nitrogen atmosphere from ambient temperature to 750°C. The experimental results revealed that the investigated complexes contain hydrated water molecules...
April 9, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28443028/improved-model-of-proton-pump-crystal-structure-obtained-by-interactive-molecular-dynamics-flexible-fitting-expands-the-mechanistic-model-for-proton-translocation-in-p-type-atpases
#9
Dorota Focht, Tristan I Croll, Bjorn P Pedersen, Poul Nissen
The plasma membrane H(+)-ATPase is a proton pump of the P-type ATPase family and essential in plants and fungi. It extrudes protons to regulate pH and maintains a strong proton-motive force that energizes e.g., secondary uptake of nutrients. The only crystal structure of a H(+)-ATPase (AHA2 from Arabidopsis thaliana) was reported in 2007. Here, we present an improved atomic model of AHA2, obtained by a combination of model rebuilding through interactive molecular dynamics flexible fitting (iMDFF) and structural refinement based on the original data, but using up-to-date refinement methods...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28421861/the-plant-dependent-life-cycle-of-thecaphora-thlaspeos-a-smut-fungus-adapted-to-brassicaceae
#10
Lamprinos Frantzeskakis, Kaitlyn J Courville, Lesley Plücker, Ronny Kellner, Julia Kruse, Andreas Brachmann, Michael Feldbrügge, Vera Göhre
Smut fungi are globally distributed plant pathogens that infect agriculturally important crop plants such as maize or potato. To date, molecular studies on plant responses to smut fungi are challenging due to the genetic complexity of their host plants. Therefore, we set out to investigate the known smut fungus of Brassicaceae hosts, Thecaphora thlaspeos. T. thlaspeos infects different Brassicaceae plant species throughout Europe, including the perennial model plant Arabis alpina. In contrast to characterized smut fungi, mature and dry T...
April 2017: Molecular Plant-microbe Interactions: MPMI
https://www.readbyqxmd.com/read/28376722/evolutionary-gradient-of-predicted-nuclear-localization-signals-nls-bearing-proteins-in-genomes-of-family-planctomycetaceae
#11
Min Guo, Ruifu Yang, Chen Huang, Qiwen Liao, Guangyi Fan, Chenghang Sun, Simon Ming-Yuen Lee
BACKGROUND: The nuclear envelope is considered a key classification marker that distinguishes prokaryotes from eukaryotes. However, this marker does not apply to the family Planctomycetaceae, which has intracellular spaces divided by lipidic intracytoplasmic membranes (ICMs). Nuclear localization signal (NLS), a short stretch of amino acid sequence, destines to transport proteins from cytoplasm into nucleus, and is also associated with the development of nuclear envelope. We attempted to investigate the NLS motifs in Planctomycetaceae genomes to demonstrate the potential molecular transition in the development of intracellular membrane system...
April 4, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28376389/identification-and-characterization-of-the-verticillin-biosynthetic-gene-cluster-in-clonostachys-rogersoniana
#12
Ying Wang, Pengjie Hu, Yuanyuan Pan, Yaxin Zhu, Xingzhong Liu, Yongsheng Che, Gang Liu
Verticillin is one of the dimeric epipolythiodioxopiperazines (ETPs) which are toxic secondary metabolites produced only by fungi. ETPs have received substantial attention since its complex molecular architecture and a wide range of biological activities. Although biosynthesis of the monomeric gliotoxin has been studied extensively, the biosynthetic pathway of dimeric ETPs is far from being studied. To investigate the biosynthesis of dimeric ETPs and expand our understanding of their dimerization, the verticillin biosynthetic gene cluster (ver) was identified and cloned from a genomic DNA fosmid library of the Cordyceps-colonizing fungus Clonostachys rogersoniana with the designed primers based on the sequence of a nonribosomal peptide synthetase (NRPS) ChaP which was predicted to be responsible for chaetocin biosynthesis in Chaetomium virescens...
April 1, 2017: Fungal Genetics and Biology: FG & B
https://www.readbyqxmd.com/read/28373739/phylogeny-and-taxonomy-of-the-scab-and-spot-anthracnose-fungus-elsino%C3%A3-myriangiales-dothideomycetes
#13
X L Fan, R W Barreto, J Z Groenewald, J D P Bezerra, O L Pereira, R Cheewangkoon, L Mostert, C M Tian, P W Crous
Species of Elsinoë are phytopathogens causing scab and spot anthracnose on many plants, including some economically important crops such as avocado, citrus, grapevines, and ornamentals such as poinsettias, field crops and woody hosts. Disease symptoms are often easily recognisable, and referred to as signature-bearing diseases, for the cork-like appearance of older infected tissues with scab-like appearance. In some Elsinoë-host associations the resulting symptoms are better described as spot anthracnose...
June 2017: Studies in Mycology
https://www.readbyqxmd.com/read/28371918/functional-lycopene-cyclase-crua-in-cyanobacterium-arthrospira-platensis-nies-39-and-its-role-in-carotenoid-synthesis
#14
Kenjiro Sugiyama, Masashi Ebisawa, Masaharu Yamada, Yoshiki Nagashima, Hideyuki Suzuki, Takashi Maoka, Shinichi Takaichi
The genus Arthrospira is filamentous, non-nitrogen-fixing cyanobacteria that is commercially important. We identified the molecular structures of carotenoids in Arthrospira platensis NIES-39. The major carotenoid identified was β-carotene. In addition, the hydroxyl derivatives of β-cryptoxanthin and (3R,3'R)-zeaxanthin were also found to be present. The carotenoid glycosides were identified as (3R,2'S)-myxol 2'-methylpentoside and oscillol 2,2'-dimethylpentoside. The methylpentoside moiety was a mixture of fucoside and chinovoside in an approximate ratio of 1 : 4...
April 1, 2017: Plant & Cell Physiology
https://www.readbyqxmd.com/read/28338801/evolutionary-origin-of-mitochondrial-cytochrome-p450
#15
Tsuneo Omura, Osamu Gotoh
Different molecular species of cytochrome P450 (P450) are distributed between endoplasmic reticulum (microsomes) and mitochondria in animal cells. Plants and fungi have many microsomal P450s, but no mitochondrial P450 has so far been reported. To elucidate the evolutionary origin of mitochondrial P450s in animal cells, available evidence is examined, and the virtual absence of mitochondrial P450 in plants and fungi is confirmed. It is also suggested that a microsomal P450 is the ancestor of animal mitochondrial P450s...
May 1, 2017: Journal of Biochemistry
https://www.readbyqxmd.com/read/28334113/you-are-what-you-get-from-your-fungi-nitrogen-stable-isotope-patterns-in-epipactis-species
#16
Julienne M-I Schiebold, Martin I Bidartondo, Peter Karasch, Barbara Gravendeel, Gerhard Gebauer
Background and Aims: Partially mycoheterotrophic plants are enriched in 13 C and 15 N compared to autotrophic plants. Here, it is hypothesized that the type of mycorrhizal fungi found in orchid roots is responsible for variation in 15 N enrichment of leaf tissue in partially mycoheterotrophic orchids. Methods: The genus Epipactis was used as a case study and carbon and nitrogen isotope abundances of eight Epipactis species, fungal sporocarps of four Tuber species and autotrophic references were measured...
February 22, 2017: Annals of Botany
https://www.readbyqxmd.com/read/28333108/the-biology-of-pichia-membranifaciens-killer-toxins
#17
REVIEW
Ignacio Belda, Javier Ruiz, Alejandro Alonso, Domingo Marquina, Antonio Santos
The killer phenomenon is defined as the ability of some yeast to secrete toxins that are lethal to other sensitive yeasts and filamentous fungi. Since the discovery of strains of Saccharomyces cerevisiae capable of secreting killer toxins, much information has been gained regarding killer toxins and this fact has substantially contributed knowledge on fundamental aspects of cell biology and yeast genetics. The killer phenomenon has been studied in Pichia membranifaciens for several years, during which two toxins have been described...
March 23, 2017: Toxins
https://www.readbyqxmd.com/read/28299913/endophytic-fungi-harbored-in-the-root-of-sophora-tonkinensis-gapnep-diversity-and-biocontrol-potential-against-phytopathogens
#18
Yu Qun Yao, Fang Lan, Yun Ming Qiao, Ji Guang Wei, Rong Shao Huang, Liang Bo Li
This work, for the first time, investigated the diversity of endophytic fungi harbored in the xylem and phloem of the root of Sophora tonkinensis Gapnep from three geographic localities with emphasis on the influence of the tissue type and geographic locality on endophytic fungal communities and their potential as biocontrol agents against phytopathogens of Panax notoginseng. A total of 655 fungal strains representing 47 taxa were isolated. Forty-two taxa (89.4%) were identified but not five taxa (10.6%) according to morphology and molecular phylogenetics...
March 15, 2017: MicrobiologyOpen
https://www.readbyqxmd.com/read/28298352/the-interactions-of-ants-with-their-biotic-environment
#19
EDITORIAL
Guillaume Chomicki, Susanne S Renner
This special feature results from the symposium 'Ants 2016: ant interactions with their biotic environments' held in Munich in May 2016 and deals with the interactions between ants and other insects, plants, microbes and fungi, studied at micro- and macroevolutionary levels with a wide range of approaches, from field ecology to next-generation sequencing, chemical ecology and molecular genetics. In this paper, we review key aspects of these biotic interactions to provide background information for the papers of this special feature After listing the major types of biotic interactions that ants engage in, we present a brief overview of ant/ant communication, ant/plant interactions, ant/fungus symbioses, and recent insights about ants and their endosymbionts...
March 15, 2017: Proceedings. Biological Sciences
https://www.readbyqxmd.com/read/28298348/a-phylogenetic-perspective-on-the-association-between-ants-hymenoptera-formicidae-and-black-yeasts-ascomycota-chaetothyriales
#20
Marie Vasse, Hermann Voglmayr, Veronika Mayer, Cécile Gueidan, Maximilian Nepel, Leandro Moreno, Sybren de Hoog, Marc-André Selosse, Doyle McKey, Rumsaïs Blatrix
The frequency and the geographical extent of symbiotic associations between ants and fungi of the order Chaetothyriales have been highlighted only recently. Using a phylogenetic approach based on seven molecular markers, we showed that ant-associated Chaetothyriales are scattered through the phylogeny of this order. There was no clustering according to geographical origin or to the taxonomy of the ant host. However, strains tended to be clustered according to the type of association with ants: strains from ant-made carton and strains from plant cavities occupied by ants ('domatia') rarely clustered together...
March 15, 2017: Proceedings. Biological Sciences
keyword
keyword
13609
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"