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Candida parapsilosis

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https://www.readbyqxmd.com/read/27915303/candida-species-isolated-from-different-body-sites-and-their-antifungal-susceptibility-pattern-cross-analysis-of-candida-albicans-and-candida-glabrata-biofilms
#1
Valentina Cataldi, Emanuela Di Campli, Paolo Fazii, Tonino Traini, Luigina Cellini, Mara Di Giulio
Candida species are regular commensal in humans, but-especially in immunocompromised patients-they represent opportunistic pathogens giving rise to systemic infection. The aim of the present work was to isolate and characterize for their antifungal profile Candida species from different body sites and to analyze the biofilms produced by C. albicans and C. glabrata isolates. Eighty-one strains of Candida species from 77 patients were identified. Epidemiological study showed that the most isolated species were C...
December 2, 2016: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
https://www.readbyqxmd.com/read/27913142/candida-species-from-oral-cavity-of-hiv-infected-children-exhibit-reduced-virulence-factors-in-the-haart-era
#2
Maristela Barbosa Portela, Elaine Lima de Amorim, Adrielle Mangabeira Santos, José Alexandre da Rocha Curvelo, Karol de Oliveira Martins, Cauli Lima Capillé, Rosangela Maria de Araújo Soares, Gloria Fernanda Barbosa de Araújo Castro
This study aimed to assess, in vitro, the biofilm viability and the phospholipase and protease production of Candida spp. from the saliva of HIV infected children and healthy controls, and to correlate the results with the use of medical data. A total of 79 isolates were analyzed: 48 Candida albicans isolates (33/15) and 20 Candida parapsilosis sensu lato complex isolates (12/8) (from HIV/control patients, respectively), and 8 Candida krusei, 1 Candida tropicalis, 1 Candida dubliniensis and 1 Candida guilliermondii from HIV patients...
November 29, 2016: Microbial Pathogenesis
https://www.readbyqxmd.com/read/27913132/rapid-identification-of-candida-spp-frequently-involved-in-invasive-mycoses-by-using-flow-through-hybridization-and-gene-chip-fhgc-technology
#3
Chen Li, Xiurong Ding, Zhizhong Liu, Juanjuan Zhu
The incidence of invasive fungal infections in immunocompromised patients has increased in recent decades. Rapid and accurate identification of these pathogenic fungi is crucial for initiating a timely, safe, and effective antifungal therapy. Here we developed a microarray based on flow-through hybridization gene chip technology. The microarray was tested for its specificity using a panel of reference and blinded clinical isolates. The results proved that this microarray was highly discriminative, leading to the unequivocal identification of each species, including Candida famata and the highly related species Candida parapsilosis, Candida orthopsilosis, and Candida metapsilosis...
November 29, 2016: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/27897199/hydroxychavicol-a-phytochemical-targeting-cutaneous-fungal-infections
#4
Intzar Ali, Naresh Kumar Satti, Prabhu Dutt, Rajendra Prasad, Inshad Ali Khan
The present study was designed to investigate the potency of hydroxychavicol on selected cutaneous human pathogenic fungi by the use of in vitro and in vivo assays and mechanistic characterization along with toxicological effects. Hydroxychavicol consistently displayed a fungicidal effect against all fungal species tested. Inoculum concentrations over the range of 10(4) to 10(7) CFU/ml did not significantly alter its antifungal potential and time-kill curve results revealed concentration-dependent killing...
November 29, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27894040/plausible-exploitation-of-jatropha-de-oiled-seed-cake-for-lipase-and-phytase-production-and-simultaneous-detoxification-by-candida-parapsilosis-isolated-from-poultry-garbage
#5
Balakrishna Kannoju, Swaruparani Ganapathiwar, Hanumalal Nunavath, Bindu Sunkar, Bhima Bhukya
Jatropha de-oiled seed cake was explored to utilize as a basic nutrient source for Candida parapsilosis, isolated from poultry garbage and selected based on the production of lipase and phytase enzymes under submerged fermentation. At optimized parameters under solid-state fermentation, lipase and phytase activities were recorded as 1056.66±2.92 and 833±2.5U/g of substrate (U/g), respectively. Besides enzyme production, complete elimination of phorbol esters and significant phytate reduction from 6.51±0...
November 19, 2016: Bioresource Technology
https://www.readbyqxmd.com/read/27880665/the-current-treatment-landscape-candidiasis
#6
Matteo Bassetti, Maddalena Peghin, Jean-Francois Timsit
The epidemiology of Candida species infection has changed over recent decades, influenced by local hospital-related factors, patient predisposing conditions and type of antifungal agents administered. A shift from Candida albicans as the predominant pathogen towards an increasing prevalence of the species Candida glabrata and Candida parapsilosis amongst critically ill patients has been documented. Changes in Candida species distribution may impact treatment recommendations due to differences in susceptibility to antifungal agents among species...
November 2016: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/27858242/epidemiology-and-reporting-of-candidaemia-in-belgium-a-multi-centre-study
#7
C Trouvé, S Blot, M-P Hayette, S Jonckheere, S Patteet, H Rodriguez-Villalobos, F Symoens, E Van Wijngaerden, K Lagrou
The primary aim of this study was to collect national epidemiological data on candidaemia and to determine the reporting time of species identification and antifungal susceptibility in clinical practice. During a 1-year period (March 2013 until February 2014), every first Candida isolate from each episode of candidaemia was included prospectively from 30 Belgian hospitals. Identification and susceptibility testing were performed according to local procedures and isolates were sent to the National Reference Center for Mycosis...
November 17, 2016: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/27837513/t2-magnetic-resonance-for-fungal-diagnosis
#8
Fainareti N Zervou, Ioannis M Zacharioudakis, Jaclynn Kurpewski, Eleftherios Mylonakis
Rapid diagnostic methods for fungal infections are long awaited and are expected to improve outcomes through early initiation of targeted antifungal therapy. T2Candida panel is a novel qualitative diagnostic platform that was recently approved by the US Food and Drug Administration (FDA) for diagnosis of candidemia with a mean time to species identification of less than 5 h. T2Candida panel is performed on the fully automated T2Dx instrument in whole blood K2EDTA specimens and is able to detect 5 Candida spp...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27812511/transcriptional-control-of-drug-resistance-virulence-and-immune-system-evasion-in-pathogenic-fungi-a-cross-species-comparison
#9
REVIEW
Pedro Pais, Catarina Costa, Mafalda Cavalheiro, Daniela Romão, Miguel C Teixeira
Transcription factors are key players in the control of the activation or repression of gene expression programs in response to environmental stimuli. The study of regulatory networks taking place in fungal pathogens is a promising research topic that can help in the fight against these pathogens by targeting specific fungal pathways as a whole, instead of targeting more specific effectors of virulence or drug resistance. This review is focused on the analysis of regulatory networks playing a central role in the referred mechanisms in the human fungal pathogens Aspergillus fumigatus, Cryptococcus neoformans, Candida albicans, Candida glabrata, Candida parapsilosis, and Candida tropicalis...
2016: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/27810262/epidemiology-of-candida-isolates-from-intensive-care-units-in-colombia-from-2010-to-2013
#10
Gabriel Motoa, Juan Sebastián Muñoz, José Oñate, Christian José Pallares, Cristhian Hernández, María Virginia Villegas
BACKGROUND: The frequency of Candida isolates as a cause of hospital infections has risen in recent years, leading to high rates of morbidity and mortality. The knowledge of the epidemiology of those hospital acquired fungal infections is essential to implement an adequate antifungal therapy. AIMS: To describe the epidemiology of Candida infections in Intensive Care Units (ICUs) from a surveillance network in Colombia. METHODS: Information was collected from the microbiology laboratories of 20 tertiary healthcare institutions from 10 Colombian cities using the Whonet® software version 5...
October 31, 2016: Revista Iberoamericana de Micología
https://www.readbyqxmd.com/read/27799331/specific-human-and-candida-cellular-interactions-lead-to-controlled-or-persistent-infection-outcomes-during-granuloma-like-formation
#11
Barbara Misme-Aucouturier, Marjorie Albassier, Nidia Alvarez-Rueda, Patrice Le Pape
A delayed-type of multicellular process could be crucial during chronic candidiasis in determining the course of infection. This reaction, consisting of organized immune cells surrounding the pathogen, initiates an inflammatory response to avoid fungal dissemination. The goal of the present study was to examine, at an in vitro cellular scale, Candida spp. and human immune cells interaction dynamics during a long-term period. By challenging human peripheral blood immune cells from ten healthy donors with thirty-two C...
October 31, 2016: Infection and Immunity
https://www.readbyqxmd.com/read/27799206/a-multi-center-study-of-method-dependent-epidemiological-cutoff-values-ecvs-for-resistance-detection-in-candida-spp-and-aspergillus-spp-to-amphotericin-b-and-echinocandins-for-the-etest-agar-diffusion-method
#12
A Espinel-Ingroff, M Arendrup, E Cantón, S Cordoba, E Dannaoui, J García-Rodríguez, G M Gonzalez, N P Govender, E Martin-Mazuelos, C Lass-Flörl, M Lackner, M J Linares Sicilia, M A Rodriguez-Iglesias, T Pelaez, R K Shields, G Garcia-Effron, J Guinea, M Sanguinetti, J Turnidge
Method-dependent Etest epidemiological cutoff values (ECVs) are not available for susceptibility testing of either Candida or Aspergillus species with amphotericin B or echinocandins. In addition, reference caspofungin MICs for Candida spp. are unreliable. Candida and Aspergillus species wild-type (WT) Etest MIC distributions (microorganisms in a species-drug combination with no detectable phenotypic resistance) were established for 4,341 Candida albicans, 113 C. dubliniensis, 1,683 C. glabrata species complex (SC), 709 C...
October 31, 2016: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/27794247/diversity-of-spoilage-fungi-associated-with-various-french-dairy-products
#13
Lucille Garnier, Florence Valence, Audrey Pawtowski, Lizaveta Auhustsinava-Galerne, Nicolas Frotté, Riccardo Baroncelli, Franck Deniel, Emmanuel Coton, Jérôme Mounier
Yeasts and molds are responsible for dairy product spoilage, resulting in significant food waste and economic losses. Yet, few studies have investigated the diversity of spoilage fungi encountered in dairy products. In the present study, 175 isolates corresponding to 105 from various spoiled dairy products and 70 originating from dairy production environments, were identified using sequencing of the ITS region, the partial β-tubulin, calmodulin and/or EFα genes, and the D1-D2 domain of the 26S rRNA gene for filamentous fungi and yeasts, respectively...
October 21, 2016: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/27790210/differential-regulation-of-myeloid-derived-suppressor-cells-by-candida-species
#14
Anurag Singh, Felipe Lelis, Stefanie Braig, Iris Schäfer, Dominik Hartl, Nikolaus Rieber
Myeloid-derived suppressor cells (MDSCs) are innate immune cells characterized by their ability to suppress T-cell responses. Recently, we demonstrated that the human-pathogenic fungi Candida albicans and Aspergillus fumigatus induced a distinct subset of neutrophilic MDSCs. To dissect Candida-mediated MDSC induction in more depth, we studied the relative efficacy of different pathogenic non-albicans Candida species to induce and functionally modulate neutrophilic MDSCs, including C. glabrata, C. parapsilosis, C...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27752471/squamous-cell-carcinoma-mimicking-fungal-infection
#15
Ryan P Brothers, Steven D Daveluy
A 61 year old immunosuppressed male presented with a non-healing wound following a rose thorn injury. Initial cultures revealed Candida parapsilosis, however histology confirmed squamous cell carcinoma in-situ (SCCis). This case emphasizes the importance of biopsy and cultures as fungal infection and SCCis often have similar, non-specific clinical appearances.
2016: IDCases
https://www.readbyqxmd.com/read/27750452/epidemiology-of-candidemia-and-antifungal-susceptibility-in-invasive-candida-species-in-the-asia-pacific-region
#16
He Wang, Ying-Chun Xu, Po-Ren Hsueh
In the Asia-Pacific region, Candida albicans is the predominant Candida species causing invasive candidiasis/candidemia in Australia, Japan, Korea, Hong Kong, Malaysia, Singapore and Thailand whereas C. tropicalis is the most frequently encountered Candida species in Pakistan and India. Invasive isolates of C. albicans, C. parapsilosis complex and C. tropicalis remain highly susceptible to fluconazole (>90% susceptible). Fluconazole resistance (6.8-15%), isolates with the non-wild-type phenotype for itraconazole susceptibility (3...
October 2016: Future Microbiology
https://www.readbyqxmd.com/read/27746090/-epidemiology-risk-factors-and-in-vitro-susceptibility-in-candidaemia-due-to-non-candida-albicans-species
#17
Elena M Marín Martínez, Ana Isabel Aller García, Estrella Martín-Mazuelos
BACKGROUND: Invasive fungal infection (IFI) has increased in recent years due to there being a greater number of risk factors. IFI caused by Candida is the most frequent, and although Candida albicans is the most isolated species, there is currently a decrease of C. albicans and an increase of other species of the genus. AIMS: To analyse the epidemiology, risk factors, and antifungal susceptibility of blood culture isolates of non-C.albicans Candida species in our hospital in the last 12years...
October 13, 2016: Revista Iberoamericana de Micología
https://www.readbyqxmd.com/read/27741168/the-in-vitro-effectiveness-of-lactobacillus-fermentum-against-different-candida-species-compared-with-broadly-used-azoles
#18
Francesca Deidda, Angela Amoruso, Stefania Nicola, Teresa Graziano, Marco Pane, Serena Allesina, Elisabetta Raiteri, Mario Del Piano, Luca Mogna
GOALS: To investigate the possible use of Lactobacillus strains in the prophylaxis and/or adjuvant therapy of acute vulvovaginal candidiasis and other vaginal infections sustained by Candida yeasts. BACKGROUND: The incidence of Candida infections has substantially increased in recent years. Treatment of vaginal infections with lactobacilli has a long tradition, starting with Döderlein's description of the vaginal microbiota. MATERIALS AND METHODS: We assessed the activity of serially diluted fluconazole and miconazole (from 3 ng/mL to 1 mg/mL) against Candida strains...
November 2016: Journal of Clinical Gastroenterology
https://www.readbyqxmd.com/read/27739423/direct-observation-of-redox-reactions-in-candida-parapsilosis-atcc-7330-by-confocal-microscopic-studies
#19
Sowmyalakshmi Venkataraman, Shoba Narayan, Anju Chadha
Confocal microscopic studies with the resting cells of yeast, Candida parapsilosis ATCC 7330, a reportedly versatile biocatalyst for redox enzyme mediated preparation of optically pure secondary alcohols in high optical purities [enantiomeric excess (ee) up to >99%] and yields, revealed that the yeast cells had large vacuoles under the experimental conditions studied where the redox reaction takes place. A novel fluorescence method was developed using 1-(6-methoxynaphthalen-2-yl)ethanol to track the site of biotransformation within the cells...
October 14, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27727401/antifungal-activities-of-diphenyl-diselenide-and-ebselen-against-echinocandin-susceptible-and-resistant-strains-of-candida-parapsilosis
#20
Francieli Chassot, Tarcieli Pozzebon Venturini, Fernanda Baldissera Piasentin, Janio Morais Santurio, Terezinha Inez Estivalet Svidzinski, Sydney Hartz Alves
We evaluated the in vitro antifungal activity of diphenyl diselenide and ebselen against echinocandin-susceptible and -resistant strains of Candida parapsilosis using the broth microdilution method. Diphenyl diselenide (MIC range=1-8μg/mL) and ebselen (MIC range=0.25-4μg/mL) showed in vitro activity against echinocandin-susceptible isolates. However, ebselen also showed the highest antifungal activity against echinocandin-resistant strains (MIC range=0.06-4μg/mL). This study demonstrated that the antifungal potential of diphenyl diselenide and ebselen deserves further investigation using in vivo experimental protocols...
September 13, 2016: New Microbiologica
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