keyword
MENU ▼
Read by QxMD icon Read
search

Candida drug resistance

keyword
https://www.readbyqxmd.com/read/28344162/identification-of-genetic-markers-of-resistance-to-echinocandins-azoles-and-5-fluorocytosine-in-candida-glabrata-by-next-generation-sequencing-a-feasibility-study
#1
Chayanika Biswas, Sharon C-A Chen, Catriona Halliday, Karina Kennedy, E Geoffrey Playford, Deborah J Marriott, Monica A Slavin, Tania C Sorrell, Vitali Sintchenko
OBJECTIVES: Multi-antifungal drug resistance in Candida glabrata is increasing. We examined the feasibility of next generation sequencing (NGS) to investigate the presence of antifungal drug resistance markers in C. glabrata. METHODS: The antifungal susceptibility of 12 clinical isolates and one ATCC strain of C. glabrata was determined using the Sensititre YeastOne® YO10 assay. These included three isolate pairs where the second isolate of each pair had developed a rise in drug MICs...
March 23, 2017: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/28331838/bibliometric-analysis-of-literature-on-antifungal-triazole-resistance-1980-2015
#2
Waleed M Sweileh, Ansam F Sawalha, Samah Al-Jabi, Sa'ed H Zyoud
BACKGROUND: Triazole antifungal agents play an important role in the treatment of a wide range of fungal infections. Little is known about antifungal triazole drug resistance when compared to antibiotic resistance. Therefore, this study was carried out to give a bibliometric overview of literature on triazole antifungal drug resistance. METHODS: Keywords related to triazole drug class and resistance were used in a search query in the Scopus search engine. The time span was set from 1980 to 2015...
March 2017: Germs
https://www.readbyqxmd.com/read/28320713/antimicrobial-percutaneous-nephrostomy-catheters-for-the-prevention-of-renal-infections-in-vitro-studies
#3
Nylev Vargas-Cruz, Ruth A Reitzel, Joel Rosenblatt, Mohamed Jamal, Ariel D Szvalb, Anne-Marie Chaftari, Ray Hachem, Issam Raad
Purpose - Percutaneous nephrostomy (PCN) catheters are the primary method for draining ureters obstructed by malignancy and preventing a decline of the renal function. However, PCN-related infections such as pyelonephritis and urosepsis remain a significant concern. Currently no antimicrobial PCNs are available for preventing infectious complications. Vascular catheters impregnated with Minocycline/Rifampin (M/R) and M/R with Chlorhexidine coating (M/R+CHD) have previously demonstrated antimicrobial activity...
March 20, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28303122/antifungal-drug-susceptibility-of-candida-species-isolated-from-hiv-positive-patients-recruited-at-a-public-hospital-in-s%C3%A3-o-lu%C3%A3-s-maranh%C3%A3-o-brazil
#4
Ana L G Terças, Sirlei G Marques, Eduardo B Moffa, Márcia B Alves, Conceição M P S de Azevedo, Walter L Siqueira, Cristina A Monteiro
Oropharyngeal candidiasis is the most common fungal infection in hospitalized patients with acquired immune deficiency syndrome (AIDS). Its progression results in invasive infections, which are a significant cause of morbidity and mortality. This study aimed to quickly and accurately identify Candida spp. from oral mucosa of AIDS patients recruited at Presidente Vargas Hospital, in São Luís city, Brazil and to evaluate the sensitivity profile of these fungi to antifungals by using an automated system. Isolates were collected from oropharyngeal mucosa of 52 hospitalized AIDS patients, under anti-viral and antifungal therapies...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28296357/antibacterial-antifungal-and-antiviral-effects-of-three-essential-oil-blends
#5
Amandine Brochot, Angèle Guilbot, Laïla Haddioui, Christine Roques
New agents that are effective against common pathogens are needed particularly for those resistant to conventional antimicrobial agents. Essential oils (EOs) are known for their antimicrobial activity. Using the broth microdilution method, we showed that (1) two unique blends of Cinnamomum zeylanicum, Daucus carota, Eucalyptus globulus and Rosmarinus officinalis EOs (AB1 and AB2; cinnamon EOs from two different suppliers) were active against the fourteen Gram-positive and -negative bacteria strains tested, including some antibiotic-resistant strains...
March 14, 2017: MicrobiologyOpen
https://www.readbyqxmd.com/read/28289028/assessment-and-optimizations-of-candida-albicans-in-vitro-biofilm-assays
#6
Matthew B Lohse, Megha Gulati, Ashley Valle Arevalo, Adam Fishburn, Alexander D Johnson, Clarissa J Nobile
Candida albicans biofilms have a significant medical impact due to their rapid growth on implanted medical devices, their resistance to antifungal drugs, and their ability to seed disseminated infections. Biofilm assays performed in vitro allow for rapid, high-throughput screening of gene deletion libraries or antifungal compounds and typically serve as precursors to in vivo studies. Here, we compile and discuss the protocols for several recently published C. albicans biofilm in vitro assays. We also describe improved versions of these protocols as well as novel in vitro assays...
March 13, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28286016/fluconazole-prophylaxis-in-preterm-infants-a-systematic-review
#7
REVIEW
Juliana Ferreira da Silva Rios, Paulo Augusto Moreira Camargos, Luísa Petri Corrêa, Roberta Maia de Castro Romanelli
OBJECTIVE: This article aims to review the use of antifungal prophylaxis with intravenous fluconazole in premature newborns and the occurrence of Invasive Candidiasis. METHODS: This is a systematic review with search at databases: PubMed, Capes Portal, Virtual Health Library (BVS - Biblioteca Virtual em Saúde)/Lilacs, Scopus and Cochrane. The keywords used were: "Antifungal", "Candida" "Fluconazole prophylaxis" and "Preterm infants". RESULTS: Invasive Candidiasis was evaluated in all the twelve items...
March 9, 2017: Brazilian Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28274127/-development-and-evaluation-of-a-new-selective-culture-medium-kbm-anaero-rs-gnr-for-detection-of-anaerobic-gram-negative-rods
#8
Taeko Narita, Kyohei Kato, Hiroki Hanaiwa, Tetsuhiro Harada, Yumiko Funashima, Makoto Akiwa, Jun-Ichiro Sekiguchi, Zenzo Nagasawa, Tsukuru Umemura
The laboratory culture methods for isolating drug-resistant pathogens has been the gold standard in medical microbiology, and play pivotal roles in the overall management of infectious diseases. Recently, several reports have emphasized the development of antibiotics-resistance among anaerobic gram-negative rods, especially Genus Bacteroides and Prevotella. Therefore, a selective culture method to detect these pathogens is needed. We developed here the new selective culture medium, termed "KBM Anaero RS-GNR," for detecting anaerobic Gram-negative rods...
March 22, 2017: JARMAM: Journal of the Association for Rapid Method and Automation in Microbiology
https://www.readbyqxmd.com/read/28274059/-rhetoric-to-reality-efficacy-of-punica-granatum-peel-extract-on-oral%C3%A2-candidiasis-an-in-vitro-study
#9
Preethi Madugula, Sudhakara Reddy, Jyothirmai Koneru, Atla Srinivasa Rao, Rayapureddi Sruthi, Divya Teja Dalli
INTRODUCTION: Global usage of synthetic drugs inadvertently has resulted in deleterious effects and antimicrobial resistance. Phytoextarcts with therapeutic properties appear to be appropriate substitutes for synthetic drugs. Punica granatum (Pomegranate) is a fruit rich in nutraceuticals and therapeutic properties that has lead to its widespread use as folk-medicine for treating innumerable diseases. AIM: To determine the in vitro antifungal efficacy of Punica granatum peel extract against the oral Candida compared with clotrimazole...
January 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28264842/contributions-of-both-atp-binding-cassette-transporter-and-cyp51a-proteins-are-essential-for-azole-resistance-in-aspergillus-fumigatus
#10
Sanjoy Paul, Daniel Diekema, W Scott Moye-Rowley
While azole drugs targeting the biosynthesis of ergosterol are effective antifungal agents, their extensive use has led to the development of resistant organisms. Infections involving azole resistant forms of the filamentous fungus Aspergillus fumigatus are often associated with genetic changes in the cyp51A gene encoding the lanosterol α14 demethylase target enzyme. Both a sequence duplication in the cyp51A promoter (TR34) as well as a substitution mutation in the coding sequence (L98H) are required for full expression of azole resistance...
March 6, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28264443/the-structure-activity-relationship-of-pterostilbene-against-candida-albicans-biofilms
#11
Dan-Dan Hu, Ri-Li Zhang, Yong Zou, Hua Zhong, En-Sheng Zhang, Xiang Luo, Yan Wang, Yuan-Ying Jiang
Candida albicans biofilms contribute to invasive infections and dramatic drug resistance, and anti-biofilm agents are urgently needed in the clinic. Pterostilbene (PTE) is a natural plant product with potentials to be developed as an anti-biofilm agent. In this study, we evaluated the structure-activity relationship (SAR) of PTE analogues against C. albicans biofilms. XTT (Sodium 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide inner salt) reduction assay was used to evaluate the activity of the analogues against C...
February 27, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28261668/staurosporine-induces-filamentation-in-the-human-fungal-pathogen-candida-albicans-via-signaling-through-cyr1-and-protein-kinase-a
#12
Jinglin L Xie, Teresa R O'Meara, Elizabeth J Polvi, Nicole Robbins, Leah E Cowen
Protein kinases are key regulators of signal transduction pathways that participate in diverse cellular processes. In fungal pathogens, kinases regulate signaling pathways that govern drug resistance, stress adaptation, and pathogenesis. The impact of kinases on the fungal regulatory circuitry has recently garnered considerable attention in the opportunistic fungal pathogen Candida albicans, which is a leading cause of human morbidity and mortality. Complex regulatory circuitry governs the C. albicans morphogenetic transition between yeast and filamentous growth, which is a key virulence trait...
March 2017: MSphere
https://www.readbyqxmd.com/read/28261570/human-salivary-protein-histatin-5-has-potent-bactericidal-activity-against-eskape-pathogens
#13
Han Du, Sumant Puri, Andrew McCall, Hannah L Norris, Thomas Russo, Mira Edgerton
ESKAPE (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumanni, Pseudomonas aeruginosa, and Enterobacter species) pathogens have characteristic multiple-drug resistance and cause an increasing number of nosocomial infections worldwide. Peptide-based therapeutics to treat ESKAPE infections might be an alternative to conventional antibiotics. Histatin 5 (Hst 5) is a salivary cationic histidine-rich peptide produced only in humans and higher primates. It has high antifungal activity against Candida albicans through an energy-dependent, non-lytic process; but its bactericidal effects are less known...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28258218/structural-analyses-of-candida-albicans-sterol-14%C3%AE-demethylase-complexed-with-azole-drugs-address-the-molecular-basis-of-azole-mediated-inhibition-of-fungal-sterol-biosynthesis
#14
Tatiana Y Hargrove, Laura Friggeri, Zdzislaw Wawrzak, Aidong Qi, William J Hoekstra, Robert J Schotzinger, John D York, F Peter Guengerich, Galina I Lepesheva
With some advances in modern medicine (such as cancer chemotherapy, broad exposure to antibiotics, and immunosuppression) the incidence of opportunistic fungal pathogens such as Candida albicans has increased. Cases of drug resistance among these pathogens have become more frequent, requiring the development of new drugs and a better understanding of the targeted enzymes. Sterol 14α-demethylase (CYP51) is a cytochrome P450 enzyme required for biosynthesis of sterols in eukaryotic cells and the major target of clinical drugs for managing fungal pathogens, but some of the CYP51 key features important for rational drug design have remained obscure...
March 3, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28257640/risk-factors-for-candida-parapsilosis-bloodstream-infection-in-a-neonatal-intensive-care-unit-a-case-control-study
#15
Carmine Garzillo, Maria Bagattini, Lidija Bogdanović, Anna Di Popolo, Vita Dora Iula, Maria Rosaria Catania, Francesco Raimondi, Maria Triassi, Raffaele Zarrilli
BACKGROUND: Candida parapsilosis is increasingly responsible for invasive candidiasis in neonates. This study investigates phenotypic and genotypic features of C. parapsilosis microbial isolates and underlying clinical conditions associated with acquisition of C. parapsilosis in a neonatal intensive care unit (NICU) in Italy. METHODS: Identification of C. parapsilosis was performed by VITEK® 2 and MALDI TOF and confirmed by analysis of internal transcribed spacer ribosomal DNA sequences...
January 19, 2017: Italian Journal of Pediatrics
https://www.readbyqxmd.com/read/28243540/retrospective-observation-of-drug-susceptibility-of-candida-strains-in-the-years-1999-2004-and-2015
#16
Cecylia Łukaszuk, Elżbieta Krajewska-Kułak, Wojciech Kułak
INTRODUCTION: There is much literature devoted to the problem of drug resistance and decreased susceptibility of fungi to commonly used antifungals. AIM: To analyze drug susceptibility of Candida albicans and non-Candida albicans strains isolated from the hands of people without any symptoms of disease over a 16-year period. MATERIALS AND METHODS: The study included a total of 1,274 Candida-type strains isolated from the hands of people without any symptoms of disease, including: in 1999, 432 strains; in 2004, 368; and in 2015, 454 strains...
2017: PeerJ
https://www.readbyqxmd.com/read/28242548/new-azole-derivatives-showing-antimicrobial-effects-and-their-mechanism-of-antifungal-activity-by-molecular-modeling-studies
#17
İnci Selin Doğan, Selma Saraç, Suat Sari, Didem Kart, Şebnem Eşsiz Gökhan, İmran Vural, Sevim Dalkara
Azole antifungals are potent inhibitors of fungal lanosterol 14α demethylase (CYP51) and have been used for eradication of systemic candidiasis clinically. Herein we report the design, synthesis, and biological evaluation of a series of 1-phenyl/1-(4-chlorophenyl)-2-(1H-imidazol-1-yl)ethanol esters. Many of these derivatives showed fungal growth inhibition at very low concentrations. Minimal inhibition concentration (MIC) value of 15 was 0.125 μg/mL against Candida albicans. Additionally, some of our compounds, such as 19 (MIC: 0...
April 21, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28241707/-the-analysis-of-pathogens-distribution-and-drug-resistance-of-bacteria-in-sputum-samples-of-pneumoconiosis-patients-combined-with-lower-respiratory-tract-infection
#18
Y Xin, N Zhang
Objective: To guide the rational use of antibiotic drugs in clinical practice for doctors by the analysis of pathogens distribution, transition and drug resistance of bacteria in sputum samples of pneumoconiosis patients combined with lower respiratory tract infection. Methods: 2 199 qualified sputum samples from 214 pneumoconiosis patients collected from January 2008 to December 2012 were examined by bacterium or fungus education. BioMerieux API and Eastern Cowin application software were used to do bacterial identification, and K-B method recommended by the NCCLS was used to take drug sensitivity tests...
January 20, 2017: Chinese Journal of Industrial Hygiene and Occupational Diseases
https://www.readbyqxmd.com/read/28238268/aryl-alkyl-lysines-membrane-active-fungicides-that-act-against-biofilms-of-candida-albicans
#19
Chandradhish Ghosh, Vikas Yadav, Waleed Younis, Haroon Mohammad, Youssef A Hegazy, Mohamed N Seleem, Kaustuv Sanyal, Jayanta Haldar
Mortality due to pathogenic fungi has been exacerbated by the rapid development of resistance to frontline antifungal drugs. Fungicidal compounds with novel mechanisms of action are urgently needed. Aryl-alkyl-lysines, which are membrane-active small molecules, were earlier shown to be broad-spectrum antibacterial agents with potency in vitro and in vivo. Herein, we report the antifungal properties of aryl-alkyl-lysines. After identifying the most active compound (NCK-10), we tested its activity against a panel of clinically relevant pathogenic fungi and examined NCK-10's effect against immature and mature biofilms of Candida albicans...
March 14, 2017: ACS Infectious Diseases
https://www.readbyqxmd.com/read/28224196/calcium-and-oxidative-stress-mediate-perillaldehyde-induced-apoptosis-in-candida-albicans
#20
Hui Tian, Su Qu, Yanzhen Wang, Zhaoqun Lu, Man Zhang, Yeyun Gan, Peng Zhang, Jun Tian
New anti-Candida albicans drugs are needed due to the emergence of resistant cases in recent years. Perillaldehyde (PAE) is a natural monoterpenoid compound derived from Perilla frutescens. The minimum inhibitory concentration of PAE against C. albicans was 0.4 μL/mL. We aimed to elucidate the antifungal mode of action of PAE against C. albicans. The antifungal activity of PAE against C. albicans was found to correlate with an elevation in intracellular Ca(2+) and accumulation of ROS. Several downstream apoptosis events such as the disruption of mitochondrial membrane potential, phosphatidylserine externalization, cytochrome c release, and metacaspase activation were observed in PAE-treated cells...
February 21, 2017: Applied Microbiology and Biotechnology
keyword
keyword
13607
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"