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Dried blood spots

Benjamin H Goot, Jon Kaufman, Zhaoxing Pan, David W A Bourne, Francis Hickey, Mark Twite, Jeffrey Galinkin, Uwe Christians, Jeannie Zuk, Eduardo M da Cruz
OBJECTIVES: To assess if morphine pharmacokinetics are different in children with Down syndrome when compared with children without Down syndrome. DESIGN: Prospective single-center study including subjects with Down syndrome undergoing cardiac surgery (neonate to 18 yr old) matched by age and cardiac lesion with non-Down syndrome controls. Subjects were placed on a postoperative morphine infusion that was adjusted as clinically necessary, and blood was sampled to measure morphine and its metabolites concentrations...
March 15, 2018: Pediatric Critical Care Medicine
Chi-Ju Yang, Na Wei, Ming Li, Kun Xie, Jian-Qiu Li, Cheng-Gang Huang, Yong-Sheng Xiao, Wen-Hua Liu, Xi-Gui Chen
BACKGROUND: Mandatory newborn screening for metabolic disorders has not been implemented in most parts of China. Newborn mortality and morbidity could be markedly reduced by early diagnosis and treatment of inborn errors of metabolism (IEM). Methods of screening for IEM by tandem mass spectrometry (MS/MS) have been developed, and their advantages include rapid testing, high sensitivity, high specificity, high throughput, and low sample volume (a single dried blood spot). METHODS: Dried blood spots of 100,077 newborns obtained from Jining city in 2014-2015 were screened by MS/MS...
March 13, 2018: BMC Pediatrics
Morgan K Hoke, Kimberly A McCabe, Aaron A Miller, Thomas W McDade
OBJECTIVE: To validate a method for measuring endotoxin-core antibodies (EndoCAb) from dried blood spots (DBS)-drops of capillary whole blood collected and dried on filter paper-as an indicator of environmental enteropathy (EE) in infancy and early childhood. METHODS: A commercially available enzyme immunoassay kit was adapted for use with DBS, with optimized steps for sample elution. Technical validation included analysis of linearity/recovery, precision and reliability, lower limit of detection, and correspondence between matched plasma and DBS samples...
March 13, 2018: American Journal of Human Biology: the Official Journal of the Human Biology Council
Kimberly Y Won, Keri Robinson, Katy L Hamlin, Joseph Tufa, Margaret Seespesara, Ryan E Wiegand, Katherine Gass, Joseph Kubofcik, Thomas B Nutman, Patrick J Lammie, Saipale Fuimaono
BACKGROUND: Current WHO recommendations for lymphatic filariasis (LF) surveillance advise programs to implement activities to monitor for new foci of transmission after stopping mass drug administration (MDA). A current need in the global effort to eliminate LF is to standardize diagnostic tools and surveillance activities beyond the recommended transmission assessment survey (TAS). METHODOLOGY: TAS was first conducted in American Samoa in 2011 (TAS 1) and a repeat TAS was carried out in 2015 (TAS 2)...
March 9, 2018: PLoS Neglected Tropical Diseases
Charlotte Jones, Gareth J Dunseath, Jessica Lemon, Stephen D Luzio
BACKGROUND: Microsampling techniques are alternative methods to venous sampling for obtaining blood for measurement of circulating biomarkers, offering the convenience of reduced sample volume and elimination of the need for phlebotomists. Dried blood spot (DBS) microsampling methods have been used for many years while more recently a volumetric absorptive microsampling device (VAMS™) has been introduced. In diabetes mellitus, circulating C-peptide is commonly used as an indicator of endogenous insulin secretion and clinical measurement can aid in diagnosis as well as informing on therapy...
March 1, 2018: Journal of Diabetes Science and Technology
Natália B Andriguetti, Roberta Z Hahn, Lilian F Lizot, Suziane Raymundo, Jose L Costa, Kelly F da Cunha, Ramon M M Vilela, Helena M Kluck, Gilberto Schwartsmann, Marina V Antunes, Rafael Linden
BACKGROUND: Paclitaxel (PCT) is a chemotherapeutic drug widely used for the treatment of several types of tumors, and its use is associated with severe adverse events, mainly neurologic and hematopoietic toxicities. The relation between systemic exposure and clinical response to PCT was previously described, making paclitaxel a potential candidate for therapeutic drug monitoring (TDM). The use of dried blood spot (DBS) sampling could allow complex sampling schedules required for TDM of PCT...
March 4, 2018: Clinical Biochemistry
Hideaki Moteki, Yuichi Isaka, Yuji Inaba, Mitsuo Motobayashi, Shin-Ya Nishio, Satoshi Ohira, Takuya Yano, Satoshi Iwasaki, Tanri Shiozawa, Kenichi Koike, Shin-Ichi Usami
OBJECTIVE: Congenital cytomegalovirus (cCMV) infection is the most common congenital infection, with the majority of infected newborns having no detectable signs. The aim of this study was to examine the accuracy of our newly developed DBS-based assay as an appropriate mass screening method for cCMV infection. METHODS: Between May 2011 and October 2016, newborns delivered at six hospitals in Nagano Prefecture, Japan were enrolled prospectively. We employed dried blood spot (DBS)-based assays with real-time quantitative PCR (qPCR)...
March 7, 2018: Acta Oto-laryngologica
Mary J Reust, Myung Hee Lee, Jenny Xiang, Wei Zhang, Dong Xu, Tatiana Batson, Tuo Zhang, Jennifer A Downs, Kathryn M Dupnik
Obtaining RNA from clinical samples collected in resource-limited settings can be costly and challenging. The goals of this study were to 1) optimize messenger RNA extraction from dried blood spots (DBS) and 2) determine how transcriptomes generated from DBS RNA compared with whole blood RNA isolated from blood collected in Tempus tubes. We studied paired samples collected from eight adults in rural Tanzania. Venous blood was collected on Whatman 903 Protein Saver cards and in tubes with RNA preservation solution...
March 5, 2018: American Journal of Tropical Medicine and Hygiene
Sanne M Kloosterboer, Brenda C M de Winter, Soma Bahmany, Linda Al-Hassany, Annet Dekker, G C Dieleman, Teun van Gelder, Bram Dierckx, Birgit C P Koch
BACKGROUND: Dried Blood Spot (DBS) sampling offers a minimally invasive sampling method for therapeutic drug monitoring of antipsychotics. To facilitate implementation in clinical practice, the aim of this study was to perform a clinical validation study of a DBS method for quantification of risperidone, aripiprazole, pipamperone and its major metabolites 9-OH risperidone and dehydro-aripiprazole in a real-life, clinical setting. METHODS: Paired DBS and venous plasma samples were analyzed (n=35 for risperidone, n=21 for aripiprazole, n=21 for pipamperone)...
March 2, 2018: Therapeutic Drug Monitoring
Xiaoliang Cheng, Kunhong Liu, Yong Liu, Maoyi Wang, Ying Ma
A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for quantification of caspofungin in dried blood spots (DBS) was developed and validated. The DBS samples, which were prepared by spotting whole blood onto Whatman 903 filter paper and drying at room temperature, were extracted with 50% methanol and further cleaned by protein precipitation with acetonitrile. Roxithromycin was selected as internal standard, and the separation of the analytes with endogenous ingredients was accomplished on a Hypersil GOLD aQ column with mobile phase composed of 0...
March 5, 2018: Rapid Communications in Mass Spectrometry: RCM
Gesmar R S Segundo, Anh T V Nguyen, Huyen T Thuc, Le N Q Nguyen, Roger H Kobayashi, Hai T Le, Huong T M Le, Troy R Torgerson, Hans D Ochs
Background: New sequencing techniques have revolutionized the identification of the molecular basis of primary immunodeficiency disorders (PID) not only by establishing a gene-based diagnosis but also by facilitating defect-specific treatment strategies, improving quality of life and survival, and allowing factual genetic counseling. Because these techniques are generally not available for physicians and their patients residing in developing countries, collaboration with overseas laboratories has been explored as a possible, albeit cumbersome, strategy...
2018: Frontiers in Immunology
Hemjot Kaur, Laura Dize, Beatriz Munoz, Charlotte Gaydos, Sheila West
PURPOSE: Serological testing for antibodies to Chlamydia trachomatis pgp3 is being evaluated as a tool to use for trachoma surveillance. There are limited data on the reproducibility of the test results using a multiplex platform. METHODS: We tested the reproducibility of a serologic test for C. trachomatis pgp3 in 6 dried blood spots collected from a random sample of 45 children from a trachoma endemic area. The spots were tested on a multiplex bead array platform, using one bead set twice, using another bead set at the same time as the first, and using the same bead set twice on different days separated by several months...
March 1, 2018: Journal of Microbiological Methods
Petter Bjornstad, Amy B Karger, David M Maahs
Accurate determination of glomerular filtration rate (GFR) is crucial for the diagnosis of kidney disease. Estimated GFR (eGFR) calculated by serum creatinine and/or cystatin C is a mainstay in clinical practice and epidemiologic research but lacks precision and accuracy until GFR <60 mL/min/1.73 m2 . Furthermore, eGFR may not precisely and accurately represent changes in GFR longitudinally. The lack of precision and accuracy is of concern in populations at high risk for kidney disease, as the dissociation between changes in eGFR and GFR may lead to missed diagnoses of early kidney disease...
January 2018: Advances in Chronic Kidney Disease
Arin E Freeman, Patrick Sullivan, Darrel Higa, Akshay Sharma, Robin MacGowan, Sabina Hirshfield, George J Greene, Laura Gravens, Pollyanna Chavez, A D McNaghten, Wayne D Johnson, Brian Mustanski
HIV testing is the gateway into both prevention and treatment services. It is important to understand how men who have sex with men (MSM) perceive HIV self-tests. We conducted focus groups and individual interviews to collect feedback on two HIV self-tests, and on a dried blood spot (DBS) specimen collection kit. Perceptions and attitudes around HIV self-testing (HIVST), and willingness to distribute HIV self-tests to others were assessed. MSM reported HIVST to be complementary to facility-based testing, and liked this approach because it offers privacy and convenience, does not require counseling, and could lead to linkage to care...
February 2018: AIDS Education and Prevention: Official Publication of the International Society for AIDS Education
Jun Kido, Hironobu Inoue, Yosuke Suzuki, Motoko Tanaka, Hiroshi Mitsubuchi, Kimitoshi Nakamura, Fumio Endo, Shirou Matsumoto
BACKGROUND: There are no reports in the literature of blood samples obtained from the same individual being subjected to analysis at the same time using the enzymatic cycling (EC) method along with electrospray tandem mass spectrometry (ESI/MS/MS) before and after carnitine treatment. METHODS: Blood samples from 29 patients (median age: 73 years old, age range: 41 - 89 years) receiving regular hemodialysis for chronic renal failure before and after carnitine treatment for 3 months were measured by the EC method, and using a dried blood spot (DBS) and ESI/MS/MS...
January 1, 2018: Clinical Laboratory
(no author information available yet)
OBJECTIVE: To generate normative data for thyroid stimulating hormone (TSH) levels in heel prick samples collected from newborns from 24 h to 7 d of age. METHODS: Five regional laboratories were designated as the testing laboratories. Dried blood spots (DBS) from babies (> or = 34 wk of gestation) were collected by heel prick at least after 24 h and within seven days after birth. TSH estimation was done using time resolved fluoroimmunoassay. Values above 20mIU/L were labelled as presumptive positive...
February 26, 2018: Indian Journal of Pediatrics
Soren Gantt, Ari Bitnun, Christian Renaud, Fatima Kakkar, Wendy Vaudry
Congenital cytomegalovirus infection (cCMV) is the most common congenital infection, occurring in approximately 0.5% of live births. Most infected newborns are asymptomatic, but up to 20% develop sensorineural hearing loss or other permanent neurologic sequelae. The presentation of newborns with symptomatic cCMV is highly variable, and the infection is usually not diagnosed in the absence of a screening program. Newborn cCMV screening programs are estimated to be beneficial and cost-effective, and are increasingly being implemented...
May 2017: Paediatrics & Child Health
Libby M Morimoto, David Zava, Katherine A McGlynn, Frank Z Stanczyk, Alice Y Kang, Xiaomei Ma, Joseph L Wiemels, Catherine Metayer
BACKGROUND: Testicular germ cell tumor (TGCT) incidence has increased over the last 40 years in the United States. In contrast to TGCT among infants, it is hypothesized that TGCT in adolescents and young men is the result of sex steroid hormone imbalance during early fetal development. However, little is known about the neonatal period when abrupt hormonal changes occur, and direct supporting evidence is scarce due to the difficulties in obtaining pre-diagnostic specimens. METHODS: We conducted a population-based case-control study examining hormone levels at birth among 91 infants (0-4 years) and 276 adolescents (15-19 years) diagnosed with TGCT, and 344 matched controls...
February 23, 2018: Cancer Epidemiology, Biomarkers & Prevention
Berit P Jensen, Rajneeta Saraf, Jing Ma, Sarah Berry, Cameron C Grant, Carlos A Camargo, Christiaan W Sies
BACKGROUND: Demand for measurement of 25-hydroxyvitamin D (25OHD) is growing and dried blood spot (DBS) sampling is attractive as samples are easier to collect, transport and store. METHODS: A 2D LC-MS/MS assay without derivatization was developed. DBS punches (3.2 mm) were ultrasonicated with d6 -25OHD3 in 70% methanol followed by hexane extraction, dry-down and reconstitution. The assay was validated and applied to two studies comparing whole blood adult DBS with serum samples (n = 40) and neonatal whole blood DBS with cord serum samples (n = 80)...
February 20, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
Sidsel Nag, Poul-Erik Kofoed, Johan Ursing, Camilla Koldbæk Lemvigh, Rosa Lundbye Allesøe, Amabelia Rodrigues, Christina Aaby Svendsen, Jacob Dyring Jensen, Michael Alifrangis, Ole Lund, Frank M Aarestrup
BACKGROUND: Plasmodium falciparum malaria remains a major health burden and genomic research represents one of the necessary approaches for continued progress towards malaria control and elimination. Sample acquisition for this purpose is troublesome, with the majority of malaria-infected individuals living in rural areas, away from main infrastructure and the electrical grid. The aim of this study was to describe a low-tech procedure to sample P. falciparum specimens for direct whole genome sequencing (WGS), without use of electricity and cold-chain...
February 23, 2018: Malaria Journal
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