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https://www.readbyqxmd.com/read/28918015/a-microrna124-target-sequence-restores-astrocyte-specificity-of-gfaabc1d-driven-transgene-expression-in-aav-mediated-gene-transfer
#1
Grit Taschenberger, Julia Tereshchenko, Sebastian Kügler
Experimentally restricting transgene expression exclusively to astrocytes has proven difficult. Using adeno-associated-virus-mediated gene transfer, we assessed two commonly used glial fibrillary acidic protein promoters: the full-length version gfa2 (2,210-bp human glial fibrillary acidic protein [GFAP] promoter) and the truncated variant gfaABC1D (681-bp GFAP promoter). The capacity to drive efficient, but also cell-type specific, expression of the EGFP in astrocytes was tested both in vitro in rat primary cortical cultures as well as in vivo in the rat striatum...
September 15, 2017: Molecular Therapy. Nucleic Acids
https://www.readbyqxmd.com/read/28916163/effects-of-small-grit-grinding-and-glazing-on-mechanical-behaviors-and-ageing-resistance-of-a-super-translucent-dental-zirconia
#2
Xuan Lai, Wenjie Si, Danyu Jiang, Ting Sun, Longquan Shao, Bin Deng
OBJECTIVES: The purpose of this study is to elucidate the effects of small-grit grinding on the mechanical behaviors and ageing resistance of a super-translucent dental zirconia and to investigate the necessity of glazing for the small-grit ground zirconia. METHODS: Small-grit grinding was performed using two kinds of silicon carbide abrasive papers. The control group received no grinding. The unground surfaces and the ground surfaces were glazed by an experienced dental technician...
September 12, 2017: Journal of Dentistry
https://www.readbyqxmd.com/read/28890864/germ-soak-water-as-nutrient-source-to-improve-fermentation-of-corn-grits-from-modified-corn-dry-grind-process
#3
Ankita Juneja, Deepak Kumar, Vijay Singh
Corn fractionation in modified dry grind processes results in low fermentation efficiency of corn grits because of nutrient deficiency. This study investigated the use of nutrient-rich water from germ soaking to improve grits fermentation in the conventional dry grind and granular starch hydrolysis (GSH) processes. Comparison of germ soak water with the use of protease and external B-vitamin addition in improving grits fermentation was conducted. Use of water from optimum soaking conditions (12 h at 30 °C) resulted in complete fermentation with 29 and 8% higher final ethanol yields compared to that of control in conventional and GSH process, respectively...
2017: Bioresources and Bioprocessing
https://www.readbyqxmd.com/read/28889402/social-freezing-in-medical-practice-experiences-and-attitudes-of-gynecologists-in-germany
#4
Maximilian Schochow, Giovanni Rubeis, Grit Büchner-Mögling, Hansjakob Fries, Florian Steger
Surveys of the German public have revealed a high acceptance of social freezing, i.e. oocyte conservation without medical indication. Up to now, there are no investigations available on the experiences and attitudes of health professionals towards social freezing. Between August 2015 and January 2016, we surveyed gynecologists Germany-wide on the topic social freezing. Five gynecologists specialized in reproductive medicine and five office-based gynecologists in standard care were chosen for the survey. The survey was conducted with an explorative, qualitative research design...
September 9, 2017: Science and Engineering Ethics
https://www.readbyqxmd.com/read/28886409/relationships-among-grit-academic-performance-perceived-academic-failure-and-stress-in-associate-degree-students
#5
Wincy Wing Sze Lee
The present study examined the relationships among grit, academic performance, perceived academic failure, and stress levels of Hong Kong associate degree students using path analysis. Three hundred and forty-five students from a community college in Hong Kong voluntarily participated in the study. They completed a questionnaire that measured their grit (operationalized as interest and perseverance) and stress levels. The students also provided their actual academic performance and evaluated their perception of their academic performance as a success or a failure...
October 2017: Journal of Adolescence
https://www.readbyqxmd.com/read/28882950/cytocompatibility-of-direct-laser-interference-patterned-titanium-surfaces-for-implants
#6
Philip Hartjen, Ola Nada, Thiago Gundelwein Silva, Clarissa Precht, Anders Henningsen, Marzellus GROßE Holthaus, Nikolai Gulow, Reinhard E Friedrich, Henning Hanken, Max Heiland, Christoph Zwahr, Ralf Smeets, Ole Jung
In an effort to generate titanium surfaces for implants with improved osseointegration, we used direct laser interference patterning (DLIP) to modify the surface of pure titanium grade 4 of four different structures. We assessed in vitro cytoxicity and cell attachment, as well as the viability and proliferation of cells cultured directly on the surfaces. Attachment of the cells to the modified surfaces was comparably good compared to that of cells on grit-blasted and acid-etched reference titanium surfaces...
September 2017: In Vivo
https://www.readbyqxmd.com/read/28868646/long-term-pulmonary-disease-among-swiss-childhood-cancer-survivors
#7
Rahel Kasteler, Annette Weiss, Matthias Schindler, Grit Sommer, Philipp Latzin, Nicolas X von der Weid, Roland A Ammann, Claudia E Kuehni
BACKGROUND: Pulmonary diseases are potentially severe late complications of childhood cancer treatment that increase mortality risk among survivors. This nationwide study assesses the prevalence and incidence of pulmonary diseases in long-term childhood cancer survivors (CCS) and their siblings, and quantifies treatment-related risks. METHODS: As part of the Swiss Childhood Cancer Survivor Study, we studied CCS who were diagnosed between 1976 and 2005 and alive at least 5 years after diagnosis...
September 4, 2017: Pediatric Blood & Cancer
https://www.readbyqxmd.com/read/28866858/sensory-physico-chemical-and-water-sorption-properties-of-corn-extrudates-enriched-with-spirulina
#8
Małgorzata Tańska, Iwona Konopka, Millena Ruszkowska
This study compares the quality of extrudates made from corn grits with the addition of up to 8% of spirulina powder. The sensory properties (shape, color, aroma, taste and crispness), chemicals (content of water, protein, fat, ash, fiber, carbohydrates, carotenoids, chlorophyll and phycocyanin) and physical properties (color, water absorption index, expansion indices, texture and water sorption properties) were determined. It has been found that spirulina-enriched extrudates had slightly lower sensory scores, but the addition of spirulina improved their nutritional value...
September 2, 2017: Plant Foods for Human Nutrition
https://www.readbyqxmd.com/read/28862070/comparative-study-on-turbulence-models-simulating-inorganic-particle-removal-in-pista-grit-chamber
#9
Zhenfei Lu, Lan Yu, Xiaoyi Xu, Fangying Ji, Chao Liu
Numerical simulations and experimental validations were conducted to determine grit removal efficiency and separation processes for inorganic suspended solids (ISS) with different particle sizes in a vortex-type grit chamber (Pista). The Euler-Lagrange method was used to simulate solid-liquid two-phase flow, the Unsteady Discrete Model was used to simulate the solid phase, and the Renormalization Group k-epsilon (RNG k-ε), Realizable k-epsilon (Real k-ε), and Shear Stress Transmission k-ω (SST k-ω) models were used to simulate the liquid phase...
September 1, 2017: Environmental Technology
https://www.readbyqxmd.com/read/28854242/evolution-and-functional-characterization-of-cazymes-belonging-to-subfamily-10-of-glycoside-hydrolase-family-5-gh5_10-in-two-species-of-phytophagous-beetles
#10
André Busch, Grit Kunert, David G Heckel, Yannick Pauchet
Hemicelluloses, such as xyloglucan, xylan and mannans, consist of a heterogeneous array of plant-derived polysaccharides that form the plant cell wall. These polysaccharides differ from each other in their structure and physiochemical properties, but they share a β-(1,4)-linked sugar backbone. Hemicelluloses can be hydrolyzed by plant-cell-wall-degrading enzymes (PCWDEs), which are widely distributed in phytopathogenic microbes. Recently, it has become apparent that phytophagous beetles also produce their own PCWDEs...
2017: PloS One
https://www.readbyqxmd.com/read/28831602/plaque-accumulation-on-titanium-disks-with-different-surface-treatments-an-in-vivo-investigation
#11
Enrico Conserva, Luigi Generali, Alberto Bandieri, Francesco Cavani, Francesco Borghi, Ugo Consolo
Implants with rough surfaces are today widely used. It has been speculated that rough surfaces (Ra > 0.2 μm) provide a better "substrate" for retention and accumulation of plaque in terms of area, thickness and colony-forming unit that can eventually lead to peri mucositis and/or peri-implantitis. The aim of this investigation was to evaluate in vivo the plaque accumulation after 48 h on three implant surfaces with different treatments. For this investigation, we used 21 sterilized titanium disks, with a diameter of 8mm and a thickness of 3 mm, provided by the manufacturer: 7 with machined surface, as smooth control, 7 with HA grit sandblasted RBM surface and 7 with Ca(++) incorporated in titanium Xpeed surface...
August 22, 2017: Odontology
https://www.readbyqxmd.com/read/28830043/reduction-and-prediction-of-n2o-emission-from-an-anoxic-oxic-wastewater-treatment-plant-upon-do-control-and-model-simulation
#12
Shichang Sun, Zhiyuan Bao, Ruoyu Li, Dezhi Sun, Haihong Geng, Xiaofei Huang, Junhao Lin, Peixin Zhang, Rui Ma, Lin Fang, Xianghua Zhang, Xuxin Zhao
In order to make a better understanding of the characteristics of N2O emission in A/O wastewater treatment plant, full-scale and pilot-scale experiments were carried out and a back propagation artificial neural network model based on the experimental data was constructed to make a precise prediction of N2O emission. Results showed that, N2O flux from different units followed a descending order: aerated grit tank>oxic zone≫anoxic zone>final clarifier>primary clarifier, but 99.4% of the total emission of N2O (1...
November 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28829600/nldft-pore-size-distribution-in-amorphous-microporous-materials
#13
Grit Kupgan, Thilanga P Liyana-Arachchi, Coray M Colina
The pore size distribution (PSD) is one of the most important properties when characterizing and designing materials for gas storage and separation applications. Experimentally, one of the current standards for determining microscopic PSD is using indirect molecular adsorption methods such as nonlocal density functional theory (NLDFT) and N2 isotherms at 77 K. Because determining the PSD from NLDFT is an indirect method, the validation can be a nontrivial task for amorphous microporous materials. This is especially crucial since this method is known to produce artifacts...
August 22, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28822319/idesia-polycarpa-salicaceae-leaf-constituents-and-their-toxic-effect-on-cerura-vinula-and-lymantria-dispar-lepidoptera-larvae
#14
Felix Feistel, Christian Paetz, Sybille Lorenz, Franziska Beran, Grit Kunert, Bernd Schneider
Phytochemical investigation of Idesia polycarpa (Salicaceae) resulted in the structure elucidation of nine previously undescribed phenolic natural products along with six known compounds. The compounds are structurally related to salicinoids that are known defense compounds from Salix and Populus species. The I. polycarpa diet was toxic, as shown in feeding experiments with larvae of Lymantria dispar, an herbivorous broadleaf tree generalist insect, and with larvae of Cerura vinula, a specialist adapted to poplar...
August 16, 2017: Phytochemistry
https://www.readbyqxmd.com/read/28803814/revision-total-hip-arthroplasty-with-a-monoblock-splined-tapered-grit-blasted-titanium-stem
#15
Michael D Hellman, Sean M Kearns, Daniel D Bohl, Bryan D Haughom, Brett R Levine
BACKGROUND: In revision total hip arthroplasty (THA), proximal femoral bone loss creates a challenge of achieving adequate stem fixation. The purpose of this study was to examine the outcomes of a monoblock, splined, tapered femoral stem in revision THA. METHODS: Outcomes of revision THA using a nonmodular, splined, tapered femoral stem from a single surgeon were reviewed. With a minimum of 2-year follow-up, there were 68 cases (67 patients). Paprosky classification was 3A or greater in 85% of the cases...
July 5, 2017: Journal of Arthroplasty
https://www.readbyqxmd.com/read/28798209/gender-math-confidence-and-grit-relationships-with-quantitative-skills-and-performance-in-an-undergraduate-biology-course
#16
K M Flanagan, J Einarson
In a world filled with big data, mathematical models, and statistics, the development of strong quantitative skills is becoming increasingly critical for modern biologists. Teachers in this field must understand how students acquire quantitative skills and explore barriers experienced by students when developing these skills. In this study, we examine the interrelationships among gender, grit, and math confidence for student performance on a pre-post quantitative skills assessment and overall performance in an undergraduate biology course...
2017: CBE Life Sciences Education
https://www.readbyqxmd.com/read/28747368/fusarium-keratitis-in-germany
#17
Grit Walther, Serena Stasch, Kerstin Kaerger, Axel Hamprecht, Mathias Roth, Oliver A Cornely, Gerd Geerling, Colin R Mackenzie, Oliver Kurzai, Marie von Lilienfeld-Toal
Fusarium keratitis is a destructive eye infection that is difficult to treat and which results in poor outcome. In the tropical and subtropical areas the infection is relatively common and associated with trauma or chronic eye diseases. However, in recent years, an increased incidence has been reported in temperate climate regions. At the German National Reference Center, we observed a steady increase in case numbers since 2014. Here, we present the first German case series of eye infections with Fusarium spp...
July 26, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28741292/effect-of-mdp-based-silane-and-different-surface-conditioner-methods-on-bonding-of-resin-cements-to-zirconium-framework
#18
Hatice Özdemir, Nuran Yanikoğlu, Nurdan Sağsöz
PURPOSE: To determine the shear bond strength (SBS) between zirconium framework and resin cements after different surface conditioner methods and after application of 10-methacryloyloxydecyl dihydrogen phosphate (MDP) based silane and a bonding agent mix. MATERIALS AND METHODS: 128 disc-shaped zirconium-oxide specimens were prepared. Specimens were placed in autopolymerizing acrylic resin. The bonding surface of specimens was smoothed consecutively with 600-, 800-, and 1200-grit silicon carbide papers under water cooling...
July 24, 2017: Journal of Prosthodontics: Official Journal of the American College of Prosthodontists
https://www.readbyqxmd.com/read/28733068/effects-of-eu-harmonization-policies-on-national-public-supervision-of-clinical-trials-a-dynamic-cycle-of-institutional-change-and-institutional-work
#19
Jacqueline C F van Oijen, Kor J Grit, Hester M van de Bovenkamp, Roland A Bal
BACKGROUND: The EU Clinical Trials Directive (EUCTD) and the EU Clinical Trials Regulation aim to harmonize good clinical practice (GCP) of clinical trials across Member States. Using the Netherlands as a case study, this paper analyzes how endeavours to implement the EUCTD set in motion a dynamic process of institutional change and institutional work. This process lead to substantial differences between policy and actual practice; therefore, it is important to learn more about the implementation of harmonization policies...
September 2017: Health Policy
https://www.readbyqxmd.com/read/28732104/reciprocal-feedback-between-self-concept-and-goal-pursuit-in-daily-life
#20
Alexander E Wong, Robin R Vallacher
OBJECTIVE: We hypothesized that self-knowledge and goal-perseverance are mutually reinforcing because of the roles of self-knowledge in directing goal pursuit, and of goal pursuit in structuring the self-concept. METHOD: To test this hypothesis, we used a daily diary design with 97 college-aged participants for 40 days to assess whether daily self-concept clarity and grit predict one another's next day levels. Data were analyzed using multilevel cross-lagged panel modeling...
July 21, 2017: Journal of Personality
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