keyword
MENU ▼
Read by QxMD icon Read
search

Manipulation

keyword
https://www.readbyqxmd.com/read/28110251/gold-nanomaterials-for-the-selective-capturing-and-sers-diagnosis-of-toxins-in-aqueous-and-biological-fluids
#1
Waleed A Hassanain, Emad L Izake, Michael S Schmidt, Godwin A Ayoko
A highly sensitive nanosensing method for the combined selective capture and SERS detection of Microcystin-LR (MC-LR) in blood plasma has been developed. The new method utilizes gold coated magnetic nanoparticles that are functionalized with anti MC-LR antibody Fab' fragments for the selective capture of MC-LR from aqueous media and blood plasma. Using an oriented immobilization approach, the Fab' fragments are covalently attached to gold surface to form a monolayer with high capture efficiency towards the toxin...
January 15, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28110236/the-predictability-of-a-partner-s-actions-modulates-the-sense-of-joint-agency
#2
Nicole K Bolt, Janeen D Loehr
When people coordinate their actions with others, they experience a sense of joint agency, i.e., shared control over actions and their consequences. The current study examined whether the predictability of others' actions modulates joint agency. Each participant coordinated with two confederate partners to produce tone sequences that matched a metronome pace. The timing of the confederates' actions was manipulated so that one partner's actions were highly predictable in time and the other's less predictable...
January 19, 2017: Cognition
https://www.readbyqxmd.com/read/28110179/analysis-the-effect-of-different-geometries-of-afm-s-cantilever-on-the-dynamic-behavior-and-the-critical-forces-of-three-dimensional-manipulation
#3
Moharam Habibnejad Korayem, Maniya B Saraie, Mahdieh B Saraee
An important challenge when using an atomic force microscope (AFM) is to be able to control the force exerted by the AFM for performing various tasks. Nevertheless, the exerted force is proportional to the deflection of the AFM cantilever, which itself is affected by a cantilever's stiffness coefficient. Many papers have been published so far on the methods of obtaining the stiffness coefficients of AFM cantilevers in 2D; however, a comprehensive model is yet to be presented on 3D cantilever motion. The discrepancies between the equations of the 2D and 3D analysis are due to the number and direction of forces and moments that are applied to a cantilever...
January 13, 2017: Ultramicroscopy
https://www.readbyqxmd.com/read/28110103/the-retromer-complex-system-in-a-transgenic-mouse-model-of-ad-influence-of-age
#4
Jin Chu, Domenico Praticò
Deficiencies of the retrograde transport mediated by the retromer complex have been described in Alzheimer's disease (AD). Genetic manipulation of retromer modulates brain amyloidosis in Tg2576 mice. However, whether the complex is altered during the development of the AD-like phenotype remains unknown. In this study we assayed the expression levels of the vacuolar sorting protein 35 (VPS35), VPS26, VPS29, and its cargo proteins, cation independent mannose 6-phosphate receptor, sortilin-related receptor in brains of Tg2576 and controls at the ages of 3, 8, and 14 months...
January 3, 2017: Neurobiology of Aging
https://www.readbyqxmd.com/read/28110021/the-application-of-crispr-technology-to-high-content-screening-in-primary-neurons
#5
Ben L Callif, Brian Maunze, Nick L Krueger, Matthew T Simpson, Murray G Blackmore
Axon growth is coordinated by multiple interacting proteins that remain incompletely characterized. High content screening (HCS), in which manipulation of candidate genes is combined with rapid image analysis of phenotypic effects, has emerged as a powerful technique to identify key regulators of axon outgrowth. Here we explore the utility of a genome editing approach referred to as CRISPR (Clustered Regularly Interspersed Palindromic Repeats) for knockout screening in primary neurons. In the CRISPR approach a DNA-cleaving Cas enzyme is guided to genomic target sequences by user-created guide RNA (sgRNA), where it initiates a double-stranded break that ultimately results in frameshift mutation and loss of protein production...
January 18, 2017: Molecular and Cellular Neurosciences
https://www.readbyqxmd.com/read/28109758/the-use-of-electronic-sensor-device-to-augment-ligament-balancing-leads-to-a-lower-rate-of-arthrofibrosis-after-total-knee-arthroplasty
#6
Jeffrey A Geller, Akshay Lakra, Taylor Murtaugh
BACKGROUND: Total knee arthroplasty (TKA) is a highly successful surgery shown to improve quality of life. One of the more common known complications of TKA is early arthrofibrosis requiring manipulation under anesthesia (MUA). This investigation evaluates the incidence of arthrofibrosis before and after the implementation of an electronic sensor device used to assist with ligament balancing. METHODS: Six hundred ninety TKAs performed without sensor use were compared to a cohort of 252 TKAs performed with sensor usage...
December 24, 2016: Journal of Arthroplasty
https://www.readbyqxmd.com/read/28109731/eating-moderates-the-link-between-body-mass-index-and-perceived-social-connection
#7
Lisa M Jaremka, Naoyuki Sunami, Megan A Nadzan
Some studies have demonstrated that heavier people perceive themselves as lacking social connections, whereas others have not. The current study investigated whether eating alters the link between body mass index (BMI) and perceived social connection, providing one explanation for inconsistencies across previous studies. Participants were instructed to refrain from eating or drinking anything except water after 9 p.m. the prior night. Upon arrival at the lab, participants were assigned to the food (n = 63) or no food (n = 110) condition...
January 18, 2017: Appetite
https://www.readbyqxmd.com/read/28109678/paclitaxel-induced-increase-in-mitochondrial-volume-mediates-dysregulation-of-intracellular-ca-2-in-putative-nociceptive-glabrous-skin-neurons-from-the-rat
#8
Eser Yilmaz, Simon C Watkins, Michael S Gold
We have recently demonstrated that in a rat model of chemotherapy-induced peripheral neuropathy (CIPN), there is a significant decrease in the duration of the depolarization-evoked Ca(2+) transient in small diameter, IB4+, and capsaicin-responsive neurons innervating the glabrous skin of the hindpaw. This change was specific to the transient duration and significantly smaller if not undetectable in neurons innervating the dorsal skin of the hindpaw or the skin of the inner thigh. Given the importance of mitochondria in intracellular Ca(2+) regulation and the findings of chemotherapy-associated increase in mitotoxicity along the sensory neuron axons, we hypothesized that CIPN is due to both increases and decreases in mitochondria function, with changes manifest in distinct subpopulations of afferents...
January 16, 2017: Cell Calcium
https://www.readbyqxmd.com/read/28109570/osteopathic-manipulative-treatment-improves-heart-surgery-outcomes-a-randomized-controlled-trial
#9
Vittorio Racca, Bruno Bordoni, Paolo Castiglioni, Maddalena Modica, Maurizio Ferratini
BACKGROUND: Controlling sternal pain after heart surgery is important to reduce the risk of postoperative complications, but pain is often undertreated because of contraindications and side effects of analgesic drugs. Recently, osteopathic manipulative treatment (OMT) was demonstrated to reduce pain in different clinical contexts, suggesting its potential utility after cardiac surgery. The aim of this open-label, controlled study is to assess whether OMT contributes to sternal pain relief and improves postoperative outcomes...
January 18, 2017: Annals of Thoracic Surgery
https://www.readbyqxmd.com/read/28109526/the-threshold-vs-lnt-showdown-dose-rate-findings-exposed-flaws-in-the-lnt-model-part-1-the-russell-muller-debate
#10
REVIEW
Edward J Calabrese
This paper assesses the discovery of the dose-rate effect in radiation genetics and how it challenged fundamental tenets of the linear non-threshold (LNT) dose response model, including the assumptions that all mutational damage is cumulative and irreversible and that the dose-response is linear at low doses. Newly uncovered historical information also describes how a key 1964 report by the International Commission for Radiological Protection (ICRP) addressed the effects of dose rate in the assessment of genetic risk...
January 18, 2017: Environmental Research
https://www.readbyqxmd.com/read/28109083/green-pharmaceutical-analysis-of-drugs-coformulated-with-highly-different-concentrations-using-spiking-and-manipulation-of-their-ratio-spectra
#11
Bassam M Ayoub
Introducing green analysis to pharmaceuticalproducts is considered a significant approach topreserving the environment. This method can be anenvironmentally friendly alternative to the existingmethods, accompanied by a validated automatedprocedure for the analysis of a drug with the lowestpossible number of samples. Different simplespectrophotometric methods were developed forthe simultaneous determination of empagliflozin(EG) and metformin (MT) by manipulating their ratiospectra in their application on a recently approvedpharmaceutical combination, Synjardy tablets...
January 20, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28108856/diagnosis-and-management-for-trigeminal-neuralgia-caused-solely-by-venous-compression
#12
Takuro Inoue, Hisao Hirai, Ayako Shima, Fumio Suzuki, Takanori Fukushima, Masayuki Matsuda
BACKGROUND: Trigeminal neuralgia (TN) caused solely by venous compression is uncommon. An appropriate vein management protocol for microvascular decompression (MVD) has not been established. We evaluated the management of veins for TN solely due to venous involvement. METHOD: We analyzed 31 patients with TN in whom only veins were manipulated during MVD. The culprit veins smaller in diameter than the vein of the cerebellopontine fissure (VCPF) were coagulated and divided in 20 patients (divided group), while transposition was performed for larger veins in the remaining 11 patients (non-divided group)...
January 20, 2017: Acta Neurochirurgica
https://www.readbyqxmd.com/read/28108840/differences-in-monoterpene-biosynthesis-and-accumulation-in-pistacia-palaestina-leaves-and-aphid-induced-galls
#13
Karin Rand, Einat Bar, Matan Ben Ari, Rachel Davidovich-Rikanati, Natalia Dudareva, Moshe Inbar, Efraim Lewinsohn
Certain insect species can induce gall formation on numerous plants species. Although the mechanism of gall development is largely unknown, it is clear that insects manipulate their hosts' anatomy, physiology, and chemistry for their own benefit. It is well known that insect-induced galls often contain vast amounts of plant defensive compounds as compared to non-colonized tissues, but it is not clear if defensive compounds can be produced in situ in the galled tissues. To answer this question, we analyzed terpene accumulation patterns and possible independent biosynthetic potential of galls induced by the aphid Baizongia pistaciae L...
January 20, 2017: Journal of Chemical Ecology
https://www.readbyqxmd.com/read/28108763/carbon-source-regulation-of-gene-expression-in-methylosinus-trichosporium-ob3b
#14
Muhammad Farhan Ul Haque, Wenyu Gu, Bipin S Baral, Alan A DiSpirito, Jeremy D Semrau
Gene expression in methanotrophs has been shown to be affected by the availability of a variety of metals, most notably copper regulating expression of alternative forms of methane monooxygenase. Here, we show that growth substrate also affects expression of genes encoding for enzymes responsible for the oxidation of methane to formaldehyde and the assimilation of carbon. Specifically, in Methylosinus trichosporium OB3b, expression of genes involved in the conversion of methane to methanol (pmoA and mmoX) and methanol to formaldehyde (mxaF, xoxF1, and xoxF2) as well as in carbon assimilation (fae1, fae2, metF, and sga) decreased when this strain was grown on methanol vs...
January 20, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28108762/enhancement-of-succinate-yield-by-manipulating-nadh-nad-ratio-and-atp-generation
#15
Jiaojiao Li, Yikui Li, Zhiyong Cui, Quanfeng Liang, Qingsheng Qi
We previously engineered Escherichia coli YL104 to efficiently produce succinate from glucose. In this study, we investigated the relationships between the NADH/NAD(+) ratio, ATP level, and overall yield of succinate production by using glucose as the carbon source in YL104. First, the use of sole NADH dehydrogenases increased the overall yield of succinate by 7% and substantially decreased the NADH/NAD(+) ratio. Second, the soluble fumarate reductase from Saccharomyces cerevisiae was overexpressed to manipulate the anaerobic NADH/NAD(+) ratio and ATP level...
January 20, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28108697/understanding-and-manipulating-meiotic-recombination-in-plants
#16
Christophe Lambing, F Christopher H Franklin, Chung-Ju Rachel Wang
No abstract text is available yet for this article.
January 20, 2017: Plant Physiology
https://www.readbyqxmd.com/read/28108492/task-context-overrules-object-and-category-related-representational-content-in-the-human-parietal-cortex
#17
Stefania Bracci, Nicky Daniels, Hans Op de Beeck
The dorsal, parietal visual stream is activated when seeing objects, but the exact nature of parietal object representations is still under discussion. Here we test 2 specific hypotheses. First, parietal cortex is biased to host some representations more than others, with a different bias compared with ventral areas. A prime example would be object action representations. Second, parietal cortex forms a general multiple-demand network with frontal areas, showing similar task effects and representational content compared with frontal areas...
January 19, 2017: Cerebral Cortex
https://www.readbyqxmd.com/read/28108395/temporal-orienting-precedes-intersensory-attention-and-has-opposing-effects-on-early-evoked-brain-activity
#18
Julian Keil, Ulrich Pomper, Nele Feuerbach, Daniel Senkowski
Intersensory attention (IA) describes the process of directing attention to a specific modality. Temporal orienting (TO) characterizes directing attention to a specific moment in time. Previously, studies indicated that these two processes could have opposite effects on early evoked brain activity. The exact time-course and processing stages of both processes are still unknown. In this human electroencephalography study, we investigated the effects of IA and TO on visuo-tactile stimulus processing within one paradigm...
January 17, 2017: NeuroImage
https://www.readbyqxmd.com/read/28108257/working-memory-load-dependent-spatio-temporal-activity-of-single-trial-p3-response-detected-with-an-adaptive-wavelet-denoiser
#19
Qiushi Zhang, Xueqian Yang, Li Yao, Xiaojie Zhao
Working memory (WM) refers to the holding and manipulation of information during cognitive tasks. Its underlying neural mechanisms have been explored through both functional magnetic resonance imaging (fMRI) and electroencephalography (EEG). Trial-by-trial coupling of simultaneously collected EEG and fMRI signals has become an important and promising approach to study the spatio-temporal dynamics of such cognitive processes. Previous studies have demonstrated a modulation effect of the WM load on both the BOLD response in certain brain areas and the amplitude of P3...
January 17, 2017: Neuroscience
https://www.readbyqxmd.com/read/28108186/editing-the-trypanosoma-cruzi-genome-with-zinc-finger-nucleases
#20
Gabriela Assis Burle-Caldas, Viviane Grazielle-Silva, Melissa Soares-Simões, Gabriela Schumann Burkard, Isabel Roditi, Wanderson Duarte DaRocha, Santuza M Teixeira
Gene function studies in Trypanosoma cruzi, the protozoan parasite that causes Chagas disease, have been hindered by the lack of efficient genetic manipulation protocols. In most organisms, insertion and deletion of DNA fragments in the genome are dependent on the generation of double-stranded DNA break (DSB) and repair. By inducing a site-specific DSB, zinc finger nucleases (ZFNs) have proven to be useful to enhance gene editing in many cell types. Using a pair of ZFNs targeted to the T. cruzi gp72 gene, we were able to generate gp72 knockout parasites with improved efficiency compared to the conventional gene knockout protocol...
January 17, 2017: Molecular and Biochemical Parasitology
keyword
keyword
1350
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"