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https://www.readbyqxmd.com/read/28636018/fluorescence-lifetime-microscopy-reveals-the-biologically-related-photophysical-heterogeneity-of-oxyblepharismin-in-light-adapted-blue-blepharisma-japonicum-cells
#1
G Checcucci, B Storti, F Ghetti, G Signore, R Bizzarri
The step-up photophobic response of the heterotrich ciliate Blepharisma japonicum is mediated by a hypericinic pigment, blepharismin, which is not present in any of the known six families of photoreceptors, namely rhodopsins, phytochromes, xanthopsins, cryptochromes, phototropins, and BLUF proteins. Upon irradiation, native cells become light-adapted (blue) by converting blepharismin into the photochemically stable oxyblepharismin (OxyBP). So far, OxyBP has been investigated mainly from a photophysical point of view in vitro, either alone or complexed with proteins...
June 21, 2017: Photochemical & Photobiological Sciences
https://www.readbyqxmd.com/read/28631647/intrahepatic-biliary-cystadenoma-with-ciliary-metaplasia-report-of-a-rare-morphological-variant
#2
Gaurav Khanna, Pragya Sharma, K S Madhusudhan, Adarsh Barwad, Piyush Ranjan, Biplab Mishra, Prasenjit Das
Histological diagnosis of biliary cystadenoma is based on the type of epithelial cells lining the cyst and radiological features. The cyst-lining cells are not usually ciliated. We herein report a very rare example of an intrahepatic biliary cystadenoma with ciliated epithelial lining, which had taken us away from this diagnosis toward an intrahepatic foregut duplication cyst. Radiologically, also the lesion was deceptive, and a possibility of hydatid cyst was considered. However, immunohistochemical workup finally led us to this diagnosis...
April 2017: Indian Journal of Pathology & Microbiology
https://www.readbyqxmd.com/read/28623687/microcalcifications-in-stone-obstructed-human-submandibular-gland-are-associated-with-apoptosis-and-cell-proliferation
#3
Ivan Lau, Ajay Potluri, Cliff-Lawrence Ibeh, Robert S Redman, Edina Paal, Bidhan C Bandyopadhyay
OBJECTIVE: Human submandibular gland (SMG) stones are associated with inflammation, fibrosis and microcalcifications in the surrounding tissues. However, there is little information about the accompanying cell injury-repair process, apoptosis, and cell proliferation. The purpose of this study was to investigate such an association and its clinical significance. DESIGN OF STUDY: Mid-gland paraffin sections of human SMGs ("stone glands") and normal SMGs ("non-stone glands") were subjected to stains for general histology (hematoxylin and eosin), fibrosis (Masson's trichrome), and calcification (alizarin red) and to immunohistochemistry for proliferative activity (Ki-67), and apoptosis (Caspase-3)...
June 7, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28623024/functional-diversity-of-aquatic-ciliates
#4
REVIEW
Thomas Weisse
This paper first reviews the concept of functional diversity in general terms and then applies it to free-living aquatic ciliates. Ciliates are extremely versatile organisms and display an enormous functional diversity as key elements of pelagic food webs, acting as predators of bacteria, algae, other protists and even some metazoans. Planktonic ciliates are important food for zooplankton, and mixotrophic and functionally autotrophic species may significantly contribute to primary production in the ocean and in lakes...
April 13, 2017: European Journal of Protistology
https://www.readbyqxmd.com/read/28621664/post-meiotic-dna-double-strand-breaks-occur-in-tetrahymena-and-require-topoisomerase-ii-and-spo11
#5
Takahiko Akematsu, Yasuhiro Fukuda, Jyoti Garg, Jeffrey S Fillingham, Ronald E Pearlman, Josef Loidl
Based on observations of markers for DNA lesions, such as phosphorylated histone H2AX (γH2AX) and open DNA ends, it has been suggested that post-meiotic DNA double-strand breaks (PM-DSBs) enable chromatin remodeling during animal spermiogenesis. However, the existence of PM-DSBs is unconfirmed, and the mechanism responsible for their formation is unclear. Here, we report the first direct observation of programmed PM-DSBs via the electrophoretic separation of DSB-generated DNA fragments in the ciliate Tetrahymena thermophila...
June 16, 2017: ELife
https://www.readbyqxmd.com/read/28621496/adrenomedullin-regulates-the-speed-of-oviductal-fluid-flow-in-cattle
#6
Yuka Yoshimoto, Takumi Nishie, Sayaka Ito, Yoshihiko Kobayashi, Yuki Yamamoto, Kiyoshi Okuda, Koji Kimura
Unidirectional flow of oviductal fluid from the ovarian to uterine side of the ampulla plays a significant role in successful pregnancy, and is produced by ciliary beating. Various systems regulate ciliary beating, such as paracrine, autocrine, and endocrine. We hypothesized that Adrenomedullin (ADM) - a peptide hormone that acts via its receptors, which are complexes of Calcitonin receptor-like receptor (CRLR) and Receptor activity-modifying protein (RAMP) 2 or 3-promotes oviductal fluid flow in the ampulla of bovine oviducts...
June 16, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/28619826/a-toolkit-for-gfp-mediated-tissue-specific-protein-degradation-in-c-elegans
#7
Shaohe Wang, Ngang Heok Tang, Pablo Lara-Gonzalez, Zhiling Zhao, Dhanya K Cheerambathur, Bram Prevo, Andrew D Chisholm, Arshad Desai, Karen Oegema
Proteins essential for embryo production, cell division, and early embryonic events are frequently re-utilized later in embryogenesis, during organismal development, or in the adult. Examining protein function across these different biological contexts requires tissue-specific perturbation. Here, we describe a method that utilizes expression of a fusion between a GFP-targeting nanobody and a SOCS-box containing ubiquitin ligase adaptor to target GFP tagged proteins for degradation. When combined with endogenous locus GFP tagging by CRISPR-Cas9 or rescue of a null mutant with a GFP fusion, this approach enables routine and efficient tissue-specific protein ablation...
June 15, 2017: Development
https://www.readbyqxmd.com/read/28615885/nyctotherus-sp-infection-in-pet-turtle-a-case-report
#8
Ajay S Satbige, Vivek R Kasaralikar, S C Halmandge, C Rajendran
The present clinical case represents the successful therapeutic management of Nyctotherus sp. protozoan infection in turtles. Two pet turtles were presented with history of diarrhea, dehydration, weight loss and passage of undigested food in the faeces. Direct faecal examination and Giemsa staining revealed the presence of cysts of Nyctotherus sp. Treatment was initiated with antiprotozoal drug, Metronidazole at the rate of 25 mg/kg body weight per-oral consecutively for 5 days, and Vimeral suspension at rate of 10-20 mg/kg per-oral for 8 days...
June 2017: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/28615872/seasonal-incidence-of-protozoan-parasitic-infestation-in-ornamental-fishes-of-west-bengal-india
#9
Mandira Saha, P K Bandyopadhyay
The communication, dealing with the prevalence of protozoan parasites of the ornamental fish of West Bengal. During the study more than five hundred fish have been examined from five districts namely, Nadia, Hooghly, Howrah, North 24-pargana and South 24-pargana of West Bengal during pre-monsoon, monsoon and post-monsoon season. The study reveals that the intensity of infection is more in case of ciliate parasites. The highest ectoparasitic infection has been recorded during post-monsoon season, i.e. from November to February, followed by the pre-monsoon period while the lowest infection was recorded during monsoon i...
June 2017: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/28610336/first-record-of-a-new-epibionts-suctorian-ciliate-tokophrya-huangmeiensis-sp-n-ciliophora-phyllopharyngea-from-redclaw-crayfish-cherax-quadricarinatus-von-martens-1868
#10
Urfa Bin Tahir, Qiong Deng, Sen Li, Yang Liu, Zhe Wang, Zemao Gu
A new suctorian ciliate, Tokophrya huangmeiensis sp. n. was isolated from the carapace of redclaw crayfish Cherax quadricarinatus, during investigation of fish ciliates diversity in freshwater aquaculture of Hubei, China. The zooids of newly isolated ciliate were found aggregated into colonies on stalks of another ciliate Epistylis. The adults of this newly found species were characterized by an elongated pyramidal shaped and corrugated cell body with two fascicles of tentacles on the apical part of cell body...
May 22, 2017: Zootaxa
https://www.readbyqxmd.com/read/28609913/some-epibiont-suctorian-ciliates-from-meiofaunal-organisms-of-maldivian-archipelago-with-description-of-a-new-ciliate-species
#11
Kapuli Gani Mohamed Thameemul Ansari, Loretta Guidi, Igor Dovgal, Maria Balsamo, Federica Semprucci
Five species of suctorian ciliates belonging to four different genera attached to meiofaunal organisms of Maldivian archipelago (Suvadiva Atoll) were documented. Three rare species (Acineta sp., Thecacineta urceolata Liao & Dovgal, 2015 and Limnoricus ceter Jankowski, 1981) and one common suctorian ciliate Thecacineta calix (Schröder, 1907) are re-described from nematodes (Croconema cinctum Cobb, 1920, Desmodorella tenuispiculum Allgén, 1928 and Desmodora sp.) and harpacticoid copepod basibionts. In addition, Trophogemma colantonii n...
May 1, 2017: Zootaxa
https://www.readbyqxmd.com/read/28609902/host-parasite-list-updating-of-ciliates-and-fermentation-in-the-digestive-tract-of-wild-miscellaneous-herbivores-in-south-africa-rsa
#12
D G Booyse, B A Dehority
This article is dedicated to the Author, Burk Dehority, who became very ill with cancer. Fortunately he confirmed or corrected all identifications on ciliates in this article. This paper was 80% finished when he became ill. He unfortunately passed away in February 2016.
May 3, 2017: Zootaxa
https://www.readbyqxmd.com/read/28609901/description-of-diploplastron-dehorityi-sp-nov-entodiniomorphida-ophryoscolecidae-a-new-rumen-ciliate-from-brazilian-sheep-ovis-aries
#13
Franciane Cedrola, Roberto Júnio Pedroso Dias, Isabel Martinele, Marta D'Agosto
To date the genus Diploplastron comprised only one species of ophryoscolecid ciliate, Diploplastron affine, which is characterized by having two retractable ciliary zones in the anterior end of the body, two slender and juxtaposed skeletal plates on the right side, a rod shape macronucleus, and two contractile vacuoles. During study on the characterization of rumen ciliate community composition in Brazilian domestic sheep, we observed ciliates with atypical morphology but with diagnostic features of genus Diploplastron...
May 3, 2017: Zootaxa
https://www.readbyqxmd.com/read/28608021/prey-diversity-effects-on-ecosystem-functioning-depend-on-consumer-identity-and-prey-composition
#14
Daniel Wohlgemuth, Joanna Filip, Helmut Hillebrand, Stefanie D Moorthi
Consumer diversity effects on ecosystem functioning are highly context dependent and are determined by consumer specialization and other consumer and prey specific traits such as growth and grazing rates. Despite complex reciprocal interactions between consumers and their prey, few experimental studies have focused on prey diversity effects on consumer dynamics and trophic transfer. In microbial microcosms, we investigated effects of algal prey diversity (one, two and four species) on the production, evenness and grazing rates of 4 ciliate consumers, differing in grazing preferences and rates...
June 13, 2017: Oecologia
https://www.readbyqxmd.com/read/28601725/morphogenesis-and-molecular-phylogeny-of-the-soil-ciliate-rigidohymena-quadrinucleata-dragesco-and-njine-1971-berger-2011-ciliophora-hypotricha-oxytrichidae
#15
Jingyi Wang, Lifang Li, Alan Warren, Chen Shao
Morphogenesis in the soil hypotrich ciliate, Rigidohymena quadrinucleata (Dragesco and Njine, 1971) Berger, 2011; was investigated using protargol staining. The main events during binary fission are as follows: (1) the proter retains the parental adoral zone of membranelles entirely, whereas the old undulating membranes dedifferentiate into an anlage that gives rise to the leftmost frontal cirrus and the new undulating membranes of the proter; (2) six streaks of frontoventral-transverse cirral anlagen are segmented in the pattern 1:3:3:3:4:4 from left to right, which form three frontal, four frontoventral, one buccal, five ventral and five transverse cirri, respectively; (3) the postoral ventral cirrus V/3 is not involved in primordia formation demonstrating that this species belongs to the stylonychines; (4) marginal anlagen are formed intrakinetally and the right marginal anlagen develop much earlier than the left ones; (5) the generation of the dorsal kineties is of typical Oxytricha-pattern and three caudal cirri are formed, one at the posterior end of each of dorsal kineties 1, 2 and 4...
April 19, 2017: European Journal of Protistology
https://www.readbyqxmd.com/read/28601408/expression-of-ciliated-bronchial-epithelium-1-during-human-spermatogenesis
#16
Christiane Pleuger, Daniela Fietz, Katja Hartmann, Hans-Christian Schuppe, Wolfgang Weidner, Sabine Kliesch, Mark Baker, Moira K O'Bryan, Martin Bergmann
OBJECTIVE: To define the precise cellular localization of ciliated bronchial epithelium 1 (CBE1) in the human testis and test its relationship to impaired spermatogenesis. DESIGN: Gene expression analysis, and histologic and immunohistochemical evaluation. SETTING: University research laboratories and andrologic outpatient clinic. PATIENT(S): Forty-three human testicular biopsies: 12 biopsies showing normal spermatogenesis (NSP), 8 with maturation arrest at level of spermatocytes (STA), 8 with maturation arrest at level of spermatids (SDA), 4 with scattered elongating spermatids, and 12 with Sertoli cell-only syndrome, with an additional 5 semen samples from healthy donors...
June 7, 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28597671/mature-teratoma-of-the-uterine-corpus-a-case-report
#17
Jan Galko, Pavel Dundr
We report a case of a 37-year old female with a mature teratoma of the uterine corpus presented by metrorrhagia. Grossly, the tumor was polypoid without apparent invasion into the myometrium. Microscopically, the tumor consisted of disorganized mature neural tissue intermingled with nodular foci of cartilaginous tissue, groups of seromucionous glands and ciliated columnar respiratory epithelium. Fifteen months after the diagnosis, the patient showed no signs of tumor relapse. Then she was lost for a follow-up...
2017: Ceskoslovenská Patologie
https://www.readbyqxmd.com/read/28597370/micropropagation-of-ajuga-species-a-mini-review
#18
REVIEW
Han Yong Park, Doo Hwan Kim, Iyyakkannu Sivanesan
The genus Ajuga L., belonging to Lamiaceae family, is widespread. The demand for Ajuga species has risen sharply because of their medicinal, ornamental, and pharmacological properties. These wide-ranging plants are being rapidly depleted due to over-collection for ornamental and medicinal purposes, as well as by habitat destruction and deforestation. Ajuga boninsimae, A. bracteosa, A. ciliate, A. genevensis, A. incisa, A. makinoi, A. multiflora, A. pyramidalis, A. shikotanensis, A. reptans, and A. vestita are categorized and protected as endangered plants...
June 8, 2017: Biotechnology Letters
https://www.readbyqxmd.com/read/28596061/ecological-assessment-of-an-algaecidal-naphthoquinone-derivate-for-the-mitigation-of-stephanodiscus-within-a-mesocosm
#19
Jae-Hyoung Joo, Zhen Kuang, Pengbin Wang, Bum Soo Park, Shailesh Kumar Patidar, Myung-Soo Han
The novel eco-friendly algaecidal naphthoquinone derivate was used to control harmful algal bloom causing species Stephanodiscus and, its effect was assessed on other undesired and non-targeted microbial communities. We conducted a mesocosm experiment to investigate the effects of this novel algaecide on native microbial communities rearing in water collected from Nakdonggang River. Upon treatment of the mesocosm with the naphthoquinone derivate the concentration of Chl-a decreased from 20.4 μg L(-1) to 9...
June 5, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28595933/morphologic-and-phylogenetic-studies-of-two-hypotrichous-ciliates-with-notes-on-morphogenesis-in-gastrostyla-steinii-engelmann-1862-ciliophora-hypotrichia
#20
Xiaotian Luo, Lifang Li, Chundi Wang, William Bourland, Xiaofeng Lin, Xiaozhong Hu
Two oxytrichid ciliates, Stylonychia (Metastylonychia) nodulinucleata Shi and Li, 1993 and Gastrostyla steinii Engelmann, 1862; collected from Huguangyan Maar Lake and Shenzhen Mangrove Nature Protection Area, southern China, respectively, were investigated using standard methods. The uncommon species Stylonychia (Metastylonychia) nodulinucleata can be recognized by its large body size, the conspicuous caudal cirri in vivo, and a moniliform macronucleus. We provide the first record of the small subunit ribosomal RNA (SSU rRNA) gene sequence for the species...
May 17, 2017: European Journal of Protistology
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