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https://www.readbyqxmd.com/read/29666785/risk-evaluation-of-endoscopic-retrograde-cholangiopancreatography-related-contrast-media-allergic-like-reaction-a-single-centre-experience
#1
Félix Trottier-Tellier, Laurence Harvey, Jean-Daniel Baillargeon
Background and Aims. Few cases of endoscopic retrograde cholangiopancreatography- (ERCP-) related contrast media (CM) adverse reactions have been reported in the current literature. There is a lack of standardisation in practice regarding premedication prophylaxis for at-risk patients undergoing ERCP and there are few data to guide the practitioners. Our goal is to evaluate the risk of CM adverse reaction in a group of patients with a past history of allergic-like reaction to iodine product undergoing ERCP...
2018: Canadian Journal of Gastroenterology & Hepatology
https://www.readbyqxmd.com/read/29665345/evaluation-of-ifn-%C3%AE-enzyme-linked-immunospot-assay-elispot-as-a-first-line-test-in-the-diagnosis-of-non-immediate-hypersensitivity-to-amoxicillin-and-penicillin
#2
Lenka Sedláčková, Miroslav Průcha, Ingrid Poláková, Blanka Míková
The current diagnostic algorithm for beta-lactam allergy is based on skin and provocation tests, both of which carry a certain risk of inducing hypersensitivity reactions. Thus, non-invasive in vitro tests reliable enough to replace skin and provocation tests at least in a portion of patients are desirable. We aimed to verify the utility of IFN-γ ELISPOT as a first-line test in patients with suspected non-immediate hypersensitivity reaction to amoxicillin (AMX) and penicillin (PNC). The prospective observational study included 24 patients with recent, suspected non-immediate hypersensitivity reaction to AMX or PNC and 6 recently-exposed healthy subjects...
2018: Prague Medical Report
https://www.readbyqxmd.com/read/29661861/forespore-targeting-of-spovd-in-bacillus-subtilis-is-mediated-by-the-n-terminal-part-of-the-protein
#3
Margareth Sidarta, Dongdong Li, Lars Hederstedt, Ewa Bukowska-Faniband
SpoVD and PBP4b are structurally very similar high molecular weight, class B, penicillin-binding proteins produced early during sporulation in Bacillus subtilis SpoVD is known to be essential for endospore cortex synthesis and thereby production of heat-resistant spores. The role of PBP4b is still enigmatic. Both proteins are synthesized in the cytoplasm of the mother cell. PBP4b remains in the cytoplasmic membrane of the mother cell whereas SpoVD accumulates in the forespore outer membrane. By the use of SpoVD/PBP4b chimeras with swapped protein domains we show that the N-terminal part of SpoVD, containing the single transmembrane region, determines forespore targeting of the protein...
April 16, 2018: Journal of Bacteriology
https://www.readbyqxmd.com/read/29659885/results-from-the-survey-of-antibiotic-resistance-soar-2014-16-in-ukraine-and-the-slovak-republic
#4
D Torumkuney, T Pertseva, E Bratus, A Dziublik, V Yachnyk, A Liskova, O Sopko, K Malynovska, I Morrissey
Objectives: To determine antibiotic susceptibility in isolates of Streptococcus pneumoniae and Haemophilus influenzae collected in 2014-16 from Ukraine and the Slovak Republic. Methods: MICs were determined by CLSI broth microdilution and susceptibility was assessed using CLSI, EUCAST and pharmacokinetic/pharmacodynamic (PK/PD) breakpoints. Results: S. pneumoniae isolates collected in Ukraine (n = 100) showed susceptibility rates ≥97% for amoxicillin, amoxicillin/clavulanic acid, penicillin [intravenous (iv) non-meningitis] and fluoroquinolones, between 83% and 86% for oral penicillin, macrolides and cefaclor, and 75% for trimethoprim/sulfamethoxazole...
April 1, 2018: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29659884/results-from-the-survey-of-antibiotic-resistance-soar-2014-16-in-greece
#5
D Torumkuney, J Papaparaskevas, I Morrissey
Objectives: To determine antimicrobial susceptibility in isolates of Streptococcus pneumoniae and Haemophilus influenzae collected in 2014-16 from patients with community-acquired respiratory tract infections in Greece. Methods: MICs were determined by CLSI broth microdilution and susceptibility assessed using CLSI, EUCAST and pharmacokinetic/pharmacodynamic (PK/PD) breakpoints. Results: A total of 99 S. pneumoniae and 52 H. influenzae isolates were collected...
April 1, 2018: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29659883/results-from-the-survey-of-antibiotic-resistance-soar-2014-16-in-the-czech-republic
#6
D Torumkuney, H Zemlickova, M Maruscak, I Morrissey
Objectives: To determine the antibiotic susceptibility of isolates of Streptococcus pneumoniae and Haemophilus influenzae collected in 2014-16 from patients with community-acquired respiratory infections in the Czech Republic. Methods: MICs were determined by CLSI broth microdilution and susceptibility was assessed using CLSI, EUCAST and pharmacokinetic/pharmacodynamic (PK/PD) breakpoints. Results: S. pneumoniae isolates (n = 200) showed high rates of susceptibility (>95%) to amoxicillin, amoxicillin/clavulanic acid, penicillin [intravenous (iv) non-meningitis], ceftriaxone, cefuroxime and the fluoroquinolones using CLSI breakpoints...
April 1, 2018: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29659882/results-from-the-survey-of-antibiotic-resistance-soar-2014-16-in-bulgaria-romania-serbia-and-croatia
#7
D Torumkuney, M Nica, I Nistor, R Vatcheva-Dobrevska, V Petrovic, A Stoica, B Hanicar, Dj Antic, I Morrissey
Objectives: To determine antibiotic susceptibility of isolates of Streptococcus pneumoniae (n = 573) and Haemophilus influenzae (n = 345) collected in 2014-16 from Bulgaria, Romania, Serbia and Croatia. Methods: MICs were determined by CLSI broth microdilution and susceptibility was assessed using CLSI, EUCAST and pharmacokinetic/pharmacodynamic (PK/PD) breakpoints. Results: Among S. pneumoniae, susceptibility was generally lowest in Romania and Serbia and highest in Bulgaria...
April 1, 2018: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29659881/results-from-the-survey-of-antibiotic-resistance-soar-2014-16-in-russia
#8
D Torumkuney, N Mayanskiy, M Edelstein, S Sidorenko, R Kozhevin, I Morrissey
Objectives: To determine antibiotic susceptibility in isolates of Streptococcus pneumoniae and Haemophilus influenzae collected in 2014-16 from Russia. Methods: MICs were determined by CLSI broth microdilution and susceptibility was assessed using CLSI, EUCAST and pharmacokinetic/pharmacodynamic (PK/PD) breakpoints. Results: A total of 279 S. pneumoniae and 279 H. influenzae were collected. Overall, 67.0% of S. pneumoniae were penicillin susceptible by CLSI oral/EUCAST and 93...
April 1, 2018: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29657402/identification-and-antibiogram-pattern-of-bacillus-cereus-from-the-milk-and-milk-products-in-and-around-jammu-region
#9
Umar Yusuf, S K Kotwal, Sanjolly Gupta, Touqeer Ahmed
Aim: The aims of the present study were to assess the prevalence, identification, and antibiogram pattern of Bacillus cereus from 215 samples of different milk and milk products in and around Jammu region. Materials and Methods: In the present study, 215 samples of milk, rasgulla, burfi, rasmalai, kalaari, paneer, ice cream, and pastry were collected and analyzed for the isolation of the B. cereus using PEMBA, and antibiogram pattern was observed for all the milk and milk products...
February 2018: Veterinary World
https://www.readbyqxmd.com/read/29652966/-molecular-basis-of-methicillin-resistance-in-staphylococcus-aureus
#10
Alejandro Aguayo-Reyes, Mario Quezada-Aguiluz, Sergio Mella, Gisela Riedel, Andrés Opazo-Capurro, Helia Bello-Toledo, Mariana Domínguez, Gerardo González-Rocha
Staphylococcus aureus isolates resistant to several antimicrobials have been gradually emerged since the beginning of the antibiotic era. Consequently, the first isolation of methicillin-resistant S. aureus occurred in 1960, which was described a few years later in Chile. Currently, S. aureus resistant to antistaphylococcal penicillins is endemic in Chilean hospitals and worldwide, being responsible for a high burden of morbidity and mortality. This resistance is mediated by the expression of a new transpeptidase, named PBP2a or PBP2', which possesses lower affinity for the β-lactam antibiotics, allowing the synthesis of peptidoglycan even in presence of these antimicrobial agents...
2018: Revista Chilena de Infectología: órgano Oficial de la Sociedad Chilena de Infectología
https://www.readbyqxmd.com/read/29652559/point-prevalence-survey-of-antibiotic-use-and-resistance-at-a-referral-hospital-in-kenya-findings-and-implications
#11
Caleb Okoth, Sylvia Opanga, Faith Okalebo, Margaret Oluka, Amanj Baker Kurdi, Brian Godman
BACKGROUND AND AIMS: A substantial amount of antibiotic use in hospitals may be inappropriate, potentially leading to the development and spread of antibiotic resistance, adverse effects, mortality and increased hospital costs. The objective was to assess current patterns of antibiotic use in a leading referral hospital in Western Kenya. This would lead to the identification of opportunities for quality improvement in this hospital and across Kenya. METHODOLOGY: A point prevalence survey was carried out with data abstracted principally from patient medical records supplemented by interviews from physicians when needed...
April 13, 2018: Hospital Practice (Minneapolis)
https://www.readbyqxmd.com/read/29651614/antibiotics-for-recurrent-acute-pharyngo-tonsillitis-systematic-review
#12
REVIEW
Holger Munck, Anders W Jørgensen, Tejs Ehlers Klug
The purpose was to determine the current evidence for preferable antibiotic treatment in three common clinical situations with insufficient consensus: Q1: Can antibiotic treatment prevent future attacks of acute pharyngo-tonsillitis (APT) in patients with recurrent APT (RAPT)? Q2: Which antibiotic regimen is preferable in the treatment of APT in patients with RAPT? Q3: Which antibiotic regimen is preferable in the treatment of relapsing APT? Five databases were searched systematically for randomized clinical trials on patients with RAPT with or without current APT or with relapse of APT...
April 13, 2018: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/29651464/hex-a-heterologous-expression-platform-for-the-discovery-of-fungal-natural-products
#13
Colin J B Harvey, Mancheng Tang, Ulrich Schlecht, Joe Horecka, Curt R Fischer, Hsiao-Ching Lin, Jian Li, Brian Naughton, James Cherry, Molly Miranda, Yong Fuga Li, Angela M Chu, James R Hennessy, Gergana A Vandova, Diane Inglis, Raeka S Aiyar, Lars M Steinmetz, Ronald W Davis, Marnix H Medema, Elizabeth Sattely, Chaitan Khosla, Robert P St Onge, Yi Tang, Maureen E Hillenmeyer
For decades, fungi have been a source of U.S. Food and Drug Administration-approved natural products such as penicillin, cyclosporine, and the statins. Recent breakthroughs in DNA sequencing suggest that millions of fungal species exist on Earth, with each genome encoding pathways capable of generating as many as dozens of natural products. However, the majority of encoded molecules are difficult or impossible to access because the organisms are uncultivable or the genes are transcriptionally silent. To overcome this bottleneck in natural product discovery, we developed the HEx (Heterologous EXpression) synthetic biology platform for rapid, scalable expression of fungal biosynthetic genes and their encoded metabolites in Saccharomyces cerevisiae ...
April 2018: Science Advances
https://www.readbyqxmd.com/read/29651413/are-there-striking-differences-in-outpatient-use-of-antibiotics-between-south-backa-district-serbia-and-some-scandinavian-countries
#14
Olga Horvat, Vesna Mijatović, Boris Milijasević, Ana Tomas, Milica Paut Kusturica, Zdenko Tomić, Ana Sabo
There is little published information about antibiotic utilization in outpatients in Serbia. The objective of this study was to determine the amount and structure of outpatient antibiotic use in South Backa District (SBD) in Serbia, to assess prescibing quality of antibiotics and to compare with results from Scandinavian countries. Data on the antibiotic use were collected from all private and state-owned pharmacies from January through March 2008 in SBD. Results were expressed as the number of defined daily doses/1,000 inhabitants/day...
2018: Frontiers in Public Health
https://www.readbyqxmd.com/read/29649700/detailed-spectral-profile-analysis-of-electrocorticograms-during-freezing-against-penicillin-induced-epileptiform-discharges-in-the-anesthetized-rat
#15
Tatsuji Tokiwa, Lev Zimin, Takao Inoue, Sadahiro Nomura, Michiyasu Suzuki, Takeshi Yamakawa
OBJECTIVES: Cryosurgery is an alternative technique for minimally invasive treatment of lesions. We have recently examined cryosurgery for epilepsy in animal models, and found that penicillin G (PG)-induced epileptiform discharges (EDs) mostly vanished after freezing. However, EDs were provoked again after insufficient freezing. Inadequate freezing is not visually detectable during and just after freezing and is not predictable beforehand. To manage this problem, we examined whether intraoperative monitoring of electrocorticograms (ECoGs) can predict recurrence of EDs after cryosurgery...
March 29, 2018: Epilepsy Research
https://www.readbyqxmd.com/read/29645291/a-simple-low-cost-and-robust-capillary-zone-electrophoresis-method-with-capacitively-coupled-contactless-conductivity-detection-for-the-routine-determination-of-four-selected-penicillins-in-money-constrained-laboratories
#16
Prasanta Paul, Cari Sänger-van de Griend, Erwin Adams, Ann Van Schepdael
A simple and robust capillary zone electrophoresis Method was developed and validated for the determination of amoxicillin and clavulanate, ampicillin, phenoxymethyl penicillin (Pen V) as well as flucloxacillin. Capacitively coupled contactless conductivity detection was employed as detection Mode that Makes CE a simple and economic tool for Money-constrained laboratories. The developed Method is straightforward and user-friendly. It offers good sensitivity and sufficient selectivity for the routine assay of the selected penicillins...
April 12, 2018: Electrophoresis
https://www.readbyqxmd.com/read/29642831/-identification-of-anaerobic-gram-negative-bacilli-isolated-from-various-clinical-specimens-and-determination-of-antibiotic-resistance-profiles-with-e-test-methods
#17
Cengiz Demir, Recep Keşli
The aim of this study was to identify gram-negative anaerobic bacilli isolated from various clinical specimens that were obtained from patients with suspected anaerobic infections and to determine the antibiotic resistance profiles by using the antibiotic concentration gradient method. The study was performed in Afyon Kocatepe University Ahmet Necdet Sezer Research and Practice Hospital, Medical Microbiology Laboratory between 1 November 2014 and 30 October 2015. Two hundred and seventyeight clinical specimens accepted for anaerobic culture were enrolled in the study...
January 2018: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/29642817/rheumatic-heart-disease-in-timor-leste-school-students-an-echocardiography-based-prevalence-study
#18
Kimberly Davis, Bo Remenyi, Anthony Dk Draper, Januario Dos Santos, Noel Bayley, Elizabeth Paratz, Benjamin Reeves, Alan Appelbe, Andrew Cochrane, Timothy D Johnson, Laura M Korte, Ivonia M Do Rosario, Inez T Da Silva Almeida, Kathryn V Roberts, Jonathan R Carapetis, Joshua R Francis
OBJECTIVES: To determine the prevalence of rheumatic heart disease (RHD) in school-aged children and young people in Timor-Leste. DESIGN: Prospective cross-sectional survey. Echocardiography was performed by Australian cardiologists to determine the presence of RHD. Demographic data were also collected. Patients in whom RHD was detected were entered into a register to allow monitoring of adherence to secondary prophylaxis; the first dose of benzathine penicillin G (BPG) was administered on the day of screening...
April 16, 2018: Medical Journal of Australia
https://www.readbyqxmd.com/read/29627075/congenital-syphilis
#19
REVIEW
Joshua M Cooper, Pablo J Sánchez
Congenital syphilis remains a major public health problem worldwide, and its incidence is increasing in the United States. This review highlights the ongoing problem of this preventable infection, and discusses vertical transmission and clinical manifestations while providing a practical algorithm for the evaluation and management of infants born to mothers with reactive serologic tests for syphilis. Every case of congenital syphilis must be seen as a failure of our public health system to provide optimal prenatal care to pregnant women, as congenital syphilis can be prevented by early and repeated prenatal serologic screening of mothers and penicillin treatment of infected women, their sexual partners, and their newborn infants...
April 4, 2018: Seminars in Perinatology
https://www.readbyqxmd.com/read/29625669/outpatient-penicillin-skin-testing-has-greater-value-in-targeted-patient-populations
#20
Viviana M Temiño, Timothy P Gauthier, Paola Lichtenberger
No abstract text is available yet for this article.
April 2018: Annals of Allergy, Asthma & Immunology
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